PRP preparation device and preparation method

Abstract

The application relates to a PRP preparation device and a preparation method. The application belongs to the technical field of medical equipment. The application specifically comprises a first centrifugal tube, a second centrifugal tube and a straw for connecting the cavities of the first centrifugal tube and the second centrifugal tube; the first centrifugal tube and the second centrifugal tube are each provided with a sealing cover which can be pierced by a needle; one end of the straw is inserted into the cavity of the first centrifugal tube, and the other end of the straw is inserted into the cavity of the second centrifugal tube; the insertion depth of the one end of the straw inserted into the first centrifugal tube can be adjusted; and the one end of the straw inserted into the second centrifugal tube is fixedly connected with the second centrifugal tube. The first centrifugal tube is used for extracting the first centrifuged target liquid in the upper layer of the first centrifugal tube into the second centrifugal tube for secondary centrifuging, so that red blood cells are not left in the first centrifuged target liquid, the problem that the red blood cells cannot be completely extracted is solved, and the first centrifugal tube and the second centrifugal tube are both in a sealed state, so that the risk that the PRP preparation process is polluted by the outside world is greatly reduced.

Description

Technical Field

[0001] This invention relates to the field of medical device technology, and in particular to a PRP preparation device and preparation method. Background Technology

[0002] PRP is an extract of autologous whole blood containing high concentrations of platelets and growth factors. Based on the white blood cell concentration, PRP is classified into two types: L-PRP (Leukocyte Platelet-Rich Plasma, containing a high concentration of white blood cells and a small number of red blood cells), primarily used for bone repair and treatment of difficult-to-heal wounds; and P-PRP (Pure Platelet-Rich Plasma, containing little or no white blood cells), which is more suitable for sports injuries and cosmetic surgery.

[0003] Existing PRP preparation methods typically employ a two-stage centrifugation process. First, the patient's blood is transferred to a centrifuge tube. The blood is then centrifuged for the first time, separating the blood into a supernatant layer and red blood cells. The red blood cells are then removed, and the remaining supernatant is centrifuged a second time. After the second centrifugation, the blood again separates into a supernatant layer and PRP. Finally, the supernatant is removed, leaving the desired PRP in the centrifuge tube. Existing PRP preparation methods have the following drawbacks: (1) When removing the red blood cells from the lower layer after the first centrifugation, residual red blood cells remain, affecting the final PRP preparation effect; (2) The open preparation system is susceptible to external contamination; (3) The complex preparation process greatly increases the risk of external contamination. Summary of the Invention

[0004] The purpose of this invention is to address the shortcomings of the prior art by providing a PRP preparation device and method that can solve the problem of residual red blood cells during PRP preparation, improve the preparation effect of PRP, and greatly reduce the risk of external contamination during PRP preparation.

[0005] This invention proposes a PRP preparation device, including a first centrifuge tube, a second centrifuge tube, and a pipette connecting the cavities of the first and second centrifuge tubes; each of the first and second centrifuge tubes has a sealing cap at one end that can be pierced by a needle; one end of the pipette is inserted into the cavity of the first centrifuge tube, and the other end is inserted into the cavity of the second centrifuge tube; the insertion depth of the pipette end inserted into the first centrifuge tube is adjustable, and the pipette end inserted into the second centrifuge tube is fixedly connected to the second centrifuge tube.

[0006] Furthermore, both the first centrifuge tube and the second centrifuge tube are conical tubes, the sealing cap seals the cavities of the first centrifuge tube and the second centrifuge tube at their pointed ends, and the straw connects the flat ends of the first centrifuge tube and the second centrifuge tube.

[0007] Furthermore, the straw is provided with external threads, and the flat end of the first centrifuge tube is provided with a threaded hole that mates with the external threads.

[0008] Furthermore, the flat end of the first centrifuge tube is provided with an opening that connects the cavity to the outside, and also includes a butyl rubber stopper that seals the opening of the flat end of the first centrifuge tube. The butyl rubber stopper is provided with an air inlet that connects the cavity of the first centrifuge tube to the outside, and the threaded hole is provided on the butyl rubber stopper.

[0009] Furthermore, an air filter is provided at the bottom of the air inlet, which is used to filter the air entering the cavity of the first centrifuge tube from the outside.

[0010] Furthermore, it also includes a fixing ring for limiting the flat ends of the first centrifuge tube and the second centrifuge tube. The flat ends of the first centrifuge tube and the second centrifuge tube are each provided with a limiting groove, and the two ends of the fixing ring are respectively inserted into the limiting grooves of the first centrifuge tube and the second centrifuge tube.

[0011] Furthermore, the fixing ring is provided with a plug to seal the air inlet.

[0012] Furthermore, the sealing cap includes a soft rubber stopper that can be pierced by a needle, and a sealing aluminum cap that fixes the soft rubber stopper to the ends of the first centrifuge tube and the second centrifuge tube, the sealing aluminum cap exposing the middle part of the soft rubber stopper.

[0013] A method for preparing PRP, using a syringe, a centrifuge, and the aforementioned PRP preparation apparatus, includes the following steps:

[0014] S1: The collected patient blood is punctured through the cap of the first centrifuge tube using a syringe and delivered into the cavity of the first centrifuge tube;

[0015] S2: Install the first centrifuge tube into the centrifuge, set the centrifugation time and centrifugation force, and then start the centrifuge.

[0016] S3: Adjust the insertion depth of the straw in the first centrifuge tube until the straw is about 1-2 mm away from the dividing layer in the first centrifuge tube;

[0017] S4: By piercing the cap of the second centrifuge tube with a syringe, the air in the cavity of the second centrifuge tube is drawn out, allowing the upper plasma in the first centrifuge tube to enter the cavity of the second centrifuge tube through a pipette.

[0018] S5: After flipping the first and second centrifuge tubes, install the second centrifuge tube into the centrifuge, reset the centrifugation time and centrifugation force, and start the centrifuge.

[0019] S6: Pierce the cap of the second centrifuge tube with a syringe and extract the PRP from the bottom layer of the second centrifuge tube.

[0020] The PRP preparation apparatus and method of the present invention have the following advantages:

[0021] (1) This preparation device extracts the first centrifugation target liquid from the upper layer of the first centrifuge tube into the second centrifuge tube, without also drawing the red blood cells from the lower layer into the second centrifuge tube, so that no red blood cells remain in the first centrifugation target liquid, solving the problem of incomplete red blood cell extraction, thereby improving the preparation effect of PRP;

[0022] (2) This preparation device injects liquid into or extracts liquid from the first and second centrifuge tubes by piercing the sealing caps of the first and second centrifuge tubes with a syringe. During this process, the first and second centrifuge tubes are sealed, thereby preventing the blood in the first and second centrifuge tubes from coming into contact with the outside air, which greatly reduces the risk of PRP preparation process being contaminated by the outside.

[0023] (3) The insertion depth of the pipette in the first centrifuge tube of this preparation device can be adjusted, so that the insertion depth of the pipette in the first centrifuge tube can be adjusted at any time according to the blood of different patients, ensuring that the height of the pipette in the first centrifuge tube is always 1-2 mm higher than the layer in the first centrifuge tube, thereby making the application range of this preparation device wider and the practicality stronger.

[0024] (4) The volume of the second centrifuge tube of this preparation device is smaller than that of the first centrifuge tube, thereby reducing the production materials required by this device in production, thus reducing the production cost of this device and saving resources;

[0025] (5) The outer wall of the straw of this preparation device is provided with external threads, and the flat end of the first centrifuge tube is provided with a threaded hole that matches the external threads on the outer wall of the straw. Thus, the insertion depth of the straw in the first centrifuge tube can be adjusted by matching the external threads on the outer wall of the straw with the threaded hole at the flat end of the first centrifuge tube.

[0026] (6) An air filter is provided at the bottom of the air inlet of this preparation device. When outside air enters the cavity of the first centrifuge tube through the air inlet, it will be filtered by the air filter, thereby reducing the pollution of the blood in the first centrifuge tube by the external environment.

[0027] (7) The two ends of the fixing ring are respectively inserted into the limiting grooves of the flat ends of the first centrifuge tube and the second centrifuge tube, so that the fixing ring limits the first centrifuge tube and the second centrifuge tube between the first centrifuge tube and the second centrifuge tube, preventing the first centrifuge tube or the second centrifuge tube from breaking under strong external force, thereby enhancing the structural strength of the device.

[0028] (8) The sealing method of fixing the soft rubber plug to the ends of the first centrifuge tube and the second centrifuge tube by sealing the aluminum cap has a better fixing and sealing effect, thereby preventing blood leakage from the first centrifuge tube and the second centrifuge tube and reducing the risk of medical accidents. Attached Figure Description

[0029] The accompanying drawings, which are incorporated in and constitute a part of this specification, illustrate embodiments of the invention and, together with the description, serve to explain the principles of the invention. In these drawings, similar reference numerals are used to denote similar elements.

[0030] Figure 1 This is a schematic diagram of a PRP preparation apparatus and preparation method according to an embodiment of the present invention;

[0031] Figure 2 This is a liquid level distribution diagram after the first centrifugation of a PRP preparation apparatus and preparation method according to an embodiment of the present invention;

[0032] Figure 3 This is a liquid level distribution diagram after the second centrifugation of a PRP preparation apparatus and method according to an embodiment of the present invention;

[0033] Figure 4 This is a schematic diagram of the structure of the first centrifuge tube of a PRP preparation device and preparation method according to an embodiment of the present invention;

[0034] Figure 5 This is a schematic diagram of the structure of the second centrifuge tube in a PRP preparation apparatus and method according to an embodiment of the present invention.

[0035] Figure 6 This is a schematic diagram of the structure of a butyl rubber stopper according to a PRP preparation apparatus and preparation method of the present invention.

[0036] Figure 7 This is a schematic diagram of the structure of the fixing ring in a PRP preparation device and preparation method according to an embodiment of the present invention.

[0037] In the diagram: 1. First centrifuge tube; 11. Limiting groove; 2. Second centrifuge tube; 3. Pipette; 31. External thread; 4. Sealing cap; 41. Soft rubber stopper; 42. Sealing aluminum cap; 5. Butyl rubber stopper; 51. Air inlet; 52. Threaded hole; 53. Air filter; 6. Fixing ring; 61. Plug; 7. Red blood cells; 8. Target solution for the first centrifugation; 9. Supernatant; 10. Target solution for the second centrifugation. Detailed Implementation

[0038] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0039] An embodiment of the present invention provides a PRP preparation device, comprising a first centrifuge tube 1, a second centrifuge tube 2, and a pipette 3 connecting the cavities of the first centrifuge tube 1 and the second centrifuge tube 2; each of the first centrifuge tube 1 and the second centrifuge tube 2 has a sealing cap 4 at one end for needle piercing; one end of the pipette 3 is inserted into the cavity of the first centrifuge tube 1, and the other end is inserted into the cavity of the second centrifuge tube 2; the insertion depth of the pipette 3 in the first centrifuge tube 1 is adjustable; the end of the pipette 3 inserted into the second centrifuge tube 2 is fixedly connected to the second centrifuge tube 2. For specific effects, see [details omitted]. Figure 1 , Figure 2 and Figure 3 .

[0040] In this application, when preparing PRP using this device, the collected patient blood is first injected into the cavity of the first centrifuge tube 1 by piercing the sealing cap 4 with a syringe. The first centrifuge tube 1 is then installed in a centrifuge, and the centrifugation time and force are set before starting the centrifuge. After the first centrifugation, the blood in the first centrifuge tube 1 separates into an upper supernatant and a lower layer of red blood cells 7, with the upper supernatant being the target solution 8 for the first centrifugation. Then, the insertion depth of the pipette 3 in the first centrifuge tube 1 is adjusted so that the height of the pipette 3 is approximately 1-2 mm higher than the separation layer in the first centrifuge tube 1. Next, the sealing cap 4 of the second centrifuge tube 2 is pierced with a syringe to remove air from the cavity of the second centrifuge tube 2, allowing the target solution 8 for the first centrifugation in the first centrifuge tube 1 to enter the cavity of the second centrifuge tube 2 through the pipette 3.

[0041] Compared to existing PRP preparation devices, after the first centrifugation, the lower layer of red blood cells 7 is extracted from the centrifuge tube, while the target solution 8 of the first centrifugation is retained in the centrifuge tube. During the extraction of the lower layer of red blood cells 7, it is difficult to extract all the lower layer of red blood cells 7 without also extracting the target solution 8 of the first centrifugation. At the same time, the liquid level of the lower layer of red blood cells 7 drops during extraction, and the liquid level of the target solution 8 of the first centrifugation drops simultaneously, replacing the previous liquid level of red blood cells 7. Since some red blood cells 7 are still left on the wall of the centrifuge tube, when the liquid level of the target solution 8 of the first centrifugation replenishes the liquid level of red blood cells 7, they will merge with the red blood cells 7 remaining on the tube wall. As a result, after the first centrifugation, the lower layer of red blood cells 7 is not completely extracted, and some remain in the target solution 8 of the first centrifugation, thus affecting the final preparation effect of PRP.

[0042] In this application, after the first centrifugation, the target liquid 8 from the upper layer of the first centrifuge tube 1 is drawn into the second centrifuge tube 2. Since the height of the pipette 3 is about 1-2 mm away from the dividing layer in the first centrifuge tube 1, the lower layer of red blood cells 7 will not be drawn into the second centrifuge tube 2 when the target liquid 8 is drawn. This ensures that no red blood cells 7 remain in the target liquid 8, solves the problem of incomplete removal of red blood cells 7, and improves the preparation effect of PRP.

[0043] After the first centrifugation of the target liquid 8 into the second centrifuge tube 2, the first centrifuge tube 1 and the second centrifuge tube 2 are inverted, and the second centrifuge tube 2 is then installed into the centrifuge. The centrifugation time and centrifugation force are reset, and the centrifuge is started. After the second centrifugation, the blood in the second centrifuge tube 2 separates into an upper supernatant 9 and a lower PRP layer, with the lower PRP layer being the second centrifugation target liquid 10. Finally, the second centrifugation target liquid 10 is extracted from the second centrifuge tube 2 by piercing the sealing cap 4 with a syringe, thus completing the preparation of PRP.

[0044] It is foreseeable that since the first centrifuge tube 1 and the second centrifuge tube 2 are connected through the pipette 3, when the first centrifuge tube 1 is installed in the centrifuge for centrifugation, the second centrifuge tube 2 will remain connected to the first centrifuge tube 1. That is, the first centrifuge tube 1 and the second centrifuge tube 2 will be centrifuged in the centrifuge at the same time. Similarly, when the second centrifuge tube 2 is installed in the centrifuge for centrifugation, the first centrifuge tube 1 will also be centrifuged in the centrifuge together with the second centrifuge tube 2.

[0045] Compared to existing PRP preparation devices, which involve drilling holes in centrifuge tubes and inserting needles into them to transfer blood, extract the lower red blood cells after the first centrifugation, and extract the PRP after the second centrifugation, the centrifuge tubes are exposed to the external environment during these operations, greatly increasing the risk of external contamination during the PRP preparation process.

[0046] In this application, blood is delivered into the first centrifuge tube 1 by piercing its sealing cap 4 with a syringe. The syringe then pierces the sealing cap 4 of the second centrifuge tube 2, removing air from its cavity and drawing the first centrifugation target liquid 8 into it. Finally, the syringe pierces the sealing cap 4 of the second centrifuge tube 2, drawing out the second centrifugation target liquid 10. During this process, both the first and second centrifuge tubes remain sealed, preventing the blood in both tubes from contacting the outside air and significantly reducing the risk of contamination during PRP preparation.

[0047] In this embodiment, both the first centrifuge tube 1 and the second centrifuge tube 2 are conical tubes. The sealing cap 4 seals the cavities of the first centrifuge tube 1 and the second centrifuge tube 2 at their pointed ends. The straw 3 connects the flat ends of the first centrifuge tube 1 and the second centrifuge tube 2. See [link to specific effect description] for details. Figure 1 Both the first centrifuge tube 1 and the second centrifuge tube 2 are designed as conical tubes because conical tubes provide the best centrifugation effect in a centrifuge, thus improving the preparation effect of PRP. The flat ends of the first centrifuge tube 1 and the second centrifuge tube 2 are positioned opposite each other, and a pipette 3 connects the flat ends of the first centrifuge tube 1 and the second centrifuge tube 2, allowing the target liquid 8 from the first centrifuge tube 1 to enter the second centrifuge tube 2 through the pipette 3. A sealing cap 4 is provided at the pointed end of both the first centrifuge tube 1 and the second centrifuge tube 2, making it easier to aspirate the liquid from the first centrifuge tube 1 and the second centrifuge tube 2 after the syringe pierces the sealing cap 4.

[0048] In this embodiment, the cavity volume of the first centrifuge tube 1 can be larger than, or smaller than or equal to, the cavity volume of the second centrifuge tube 2, depending on the actual needs of use. Since the first centrifuge tube 1 is used to hold collected patient blood, and the second centrifuge tube 2 is used to hold the first centrifugation target liquid 8 separated from the blood in the first centrifuge tube 1, the amount of liquid that the second centrifuge tube 2 needs to hold is definitely less than the amount of liquid that the first centrifuge tube 1 needs to hold. Because a larger volume means more production materials are required for manufacturing, in this device, it is preferable to set the volume of the second centrifuge tube 2 to be smaller than the volume of the first centrifuge tube 1, thereby reducing the production materials required for the production of this device, thus reducing the production cost of this device and saving resources. At the same time, it also allows for the differentiation of the first centrifuge tube 1 and the second centrifuge tube 2, preventing confusion during use.

[0049] In this embodiment, the straw 3 is provided with an external thread 31, and the flat end of the first centrifuge tube 1 is provided with a threaded hole 52 that mates with the external thread 31. See [link to specific details] for details. Figure 4 and Figure 5 The straw 3 has an external thread 31 on its outer wall, and the flat end of the first centrifuge tube 1 has a threaded hole 52 that mates with the external thread 31 on the outer wall of the straw 3. Thus, by engaging the external thread 31 on the outer wall of the straw 3 with the threaded hole 52 on the flat end of the first centrifuge tube 1, the insertion depth of the straw 3 in the first centrifuge tube 1 can be adjusted. Since one end of the straw 3 is screwed to the flat end of the first centrifuge tube 1, and the other end is fixedly connected to the flat end of the second centrifuge tube 2, when it is necessary to adjust the insertion depth of the straw 3 in the first centrifuge tube 1, simply rotating the second centrifuge tube 2 will move the straw 3 up and down within the first centrifuge tube 1. This makes adjusting the insertion depth of the straw 3 in the first centrifuge tube 1 convenient and quick, thereby making the device more convenient to use.

[0050] In this application, the reason why the insertion depth of the pipette 3 in the first centrifuge tube 1 is adjustable is that the components contained in the blood of different patients are different. Therefore, even if the amount of blood delivered to the first centrifuge tube 1 is the same for different patients, the liquid level of the first centrifugation target liquid 8 in the first centrifuge tube 1 will be different after the first centrifugation. The pipette 3 should try to ensure that the first centrifugation target liquid 8 in the first centrifuge tube 1 is drawn into the second centrifuge tube 2, while ensuring that the red blood cells 7 in the first centrifuge tube 1 are not drawn into the second centrifuge tube 2. The optimal situation is that the height of the pipette 3 is about 1-2 mm higher than the dividing layer in the first centrifuge tube 1.

[0051] If the insertion length of pipette 3 in the first centrifuge tube 1 is fixed, and the same preparation device is used for different patients, the height of pipette 3 in the first centrifuge tube 1 may be greater than 1-2 mm from the layer in the first centrifuge tube 1. This may result in incomplete extraction of the target liquid 8 in the first centrifuge tube 1, thus affecting the preparation effect of PRP. Alternatively, the height of pipette 3 in the first centrifuge tube 1 may be lower than the layer in the first centrifuge tube 1, causing pipette 3 to also draw the red blood cells 7 from the lower layer of the first centrifuge tube 1 into the second centrifuge tube 2, affecting the effect of the second centrifugation.

[0052] Therefore, in this application, the insertion depth of the pipette 3 in the first centrifuge tube 1 can be adjusted. This allows the insertion depth of the pipette 3 in the first centrifuge tube 1 to be adjusted at any time according to the different patients' blood after the first centrifugation, when the liquid level of the first centrifuged target liquid 8 in the first centrifuge tube 1 changes. This ensures that the height of the pipette 3 in the first centrifuge tube 1 is always 1-2 mm higher than the dividing layer in the first centrifuge tube 1, thereby making the application range of this preparation device wider and its practicality stronger.

[0053] Since the target solution 8 from the first centrifuge tube 1 remains in the first centrifuge tube 1 after being transferred to the second centrifuge tube 2, the first and second centrifuge tubes 1 and 2 need to be inverted for the second centrifuge tube 2. It is foreseeable that after inverting the first centrifuge tube 1, the height of the pipette 3 within the first centrifuge tube 1 will be greater than the height of the red blood cell 7 liquid level, thus preventing the red blood cells 7 from entering the second centrifuge tube 2 through the pipette 3. Furthermore, due to the small diameter of the pipette 3, after inverting the first and second centrifuge tubes 1 and 2, the possibility of the red blood cells 7 in the first centrifuge tube 1 being flung into the pipette 3 during the second centrifuge is very small, thus preventing the red blood cells 7 from entering the second centrifuge tube 2 through the pipette 3.

[0054] In this embodiment, the flat end of the first centrifuge tube 1 is provided with an opening that connects the cavity to the outside. It also includes a butyl rubber stopper 5 that seals the opening at the flat end of the first centrifuge tube 1. The butyl rubber stopper 5 is provided with an air inlet 51 that connects the cavity of the first centrifuge tube 1 to the outside. A threaded hole 52 is provided on the butyl rubber stopper 5. See [link to specific details] for details. Figure 4 and Figure 6 An opening is provided at the flat end of the first centrifuge tube 1, which connects the cavity of the first centrifuge tube 1 to the outside. At the same time, a butyl rubber stopper 5 is provided at the flat end of the first centrifuge tube 1 to seal the opening. An air inlet 51 is provided on the butyl rubber stopper 5, which allows outside air to enter the first centrifuge tube 1 through the air inlet 51.

[0055] The reason for sealing the opening at the flat end of the first centrifuge tube 1 with a butyl rubber stopper 5 and providing an air inlet 51 for communication with the outside is that, in this application, air is drawn from the cavity of the second centrifuge tube 2 using a syringe, allowing the first centrifuged target liquid 8 in the first centrifuge tube 1 to enter the cavity of the second centrifuge tube 2 through a pipette 3. The reason the first centrifuged target liquid 8 enters the second centrifuge tube 2 through the pipette 3 is that after the syringe draws air from the cavity of the second centrifuge tube 2, the air pressure in the second centrifuge tube 2 becomes lower than the air pressure in the first centrifuge tube 1.

[0056] If the first centrifuge tube 1 is completely sealed, after the first centrifuged target liquid 8 in the first centrifuge tube 1 enters the second centrifuge tube 2, the air pressure in the first centrifuge tube 1 decreases, while the air pressure in the second centrifuge tube 2 increases. When the air pressure in the first centrifuge tube 1 and the air pressure in the second centrifuge tube 2 are equal, the first centrifuged target liquid 8 can no longer enter the second centrifuge tube 2, resulting in incomplete extraction of the first centrifuged target liquid 8 from the first centrifuge tube 1, thus affecting the preparation effect of PRP. Therefore, it is necessary to set an air inlet 51 on the first centrifuge tube 1 to communicate with the outside, so that when the air pressure in the first centrifuge tube 1 decreases, outside air can enter the first centrifuge tube 1 through the air inlet 51, ensuring that the air pressure in the first centrifuge tube 1 is greater than the air pressure in the second centrifuge tube 2.

[0057] It is foreseeable that, in order to prevent excessive contact between the blood in the first centrifuge tube 1 and the external environment, which could lead to contamination of the blood and affect the performance of the prepared PRP, the diameter of the air inlet 51 cannot be set too large. It is also foreseeable that, since the butyl rubber stopper 5 has a certain degree of elasticity, the pipette 3 does not necessarily need to be threaded into the butyl rubber stopper 5 in this application. The threaded hole 52 on the butyl rubber stopper 5 can also be a through hole. The elasticity of the butyl rubber stopper 5 itself can press the external thread 31 of the pipette 3 tightly. When it is necessary to adjust the insertion depth of the pipette 3 in the first centrifuge tube 1, it can be achieved by pressing down or pulling outwards the pipette 3.

[0058] In this embodiment, an air filter 53 is provided at the bottom of the air inlet 51. The air filter 53 is used to filter the air entering the cavity of the first centrifuge tube 1 from the outside. For specific effects, see [link to documentation]. Figure 6 Regardless of the size of the air inlet 51, for all the target liquid 8 from the first centrifuge tube 1 to be drawn into the second centrifuge tube 2, outside air needs to enter the first centrifuge tube 1 through the air inlet 51, thus contaminating the blood in the first centrifuge tube 1 to some extent. Therefore, in this application, an air filter 53 is provided at the bottom of the air inlet 51. When outside air enters the cavity of the first centrifuge tube 1 through the air inlet 51, it will be filtered by the air filter 53, thereby reducing the contamination of the blood in the first centrifuge tube 1 by the external environment.

[0059] In this embodiment, a fixing ring 6 is also included to limit the flat ends of the first centrifuge tube 1 and the second centrifuge tube 2. Both the flat ends of the first centrifuge tube 1 and the second centrifuge tube 2 are provided with limiting grooves 11. The two ends of the fixing ring 6 are respectively inserted into the limiting grooves 11 of the first centrifuge tube 1 and the second centrifuge tube 2. For specific effects, see [details omitted]. Figure 1 , Figure 4 and Figure 5 Both the first centrifuge tube 1 and the second centrifuge tube 2 have limiting grooves 11 at their flat ends. When installing the fixing ring 6 at the flat ends of the first centrifuge tube 1 and the second centrifuge tube 2, first rotate the second centrifuge tube 2 so that the external thread 31 on the outer wall of the suction tube 3 engages with the threaded hole 52 at the flat end of the first centrifuge tube 1, making the distance between the flat end of the second centrifuge tube 2 and the flat end of the first centrifuge tube 1 greater than the height of the fixing ring 6. Then, insert one end of the fixing ring 6 into the limiting groove 11 at the flat end of the first centrifuge tube 1. Then rotate the second centrifuge tube 2 so that the flat end of the second centrifuge tube 2 and the flat end of the first centrifuge tube 1 move closer to each other until the other end of the fixing ring 6 is inserted into the limiting groove 11 at the flat end of the second centrifuge tube 2.

[0060] When it is necessary to remove the retaining ring 6 from the flat ends of the first centrifuge tube 1 and the second centrifuge tube 2, simply rotate the second centrifuge tube 2 so that the flat ends of the second centrifuge tube 2 and the first centrifuge tube 1 are far apart, until the distance between the flat ends of the second centrifuge tube 2 and the first centrifuge tube 1 is greater than the height of the retaining ring 6, and the other end of the retaining ring 6 is no longer inserted into the limiting groove 11 of the flat end of the second centrifuge tube 2, then one end of the retaining ring 6 can be pulled out from the limiting groove 11 of the flat end of the first centrifuge tube 1, thus completing the removal of the retaining ring 6.

[0061] The fixing ring 6 is provided because the first centrifuge tube 1 and the second centrifuge tube 2 are connected only by a straw 3, and the diameter of the straw 3 is smaller than that of the first centrifuge tube 1 and the second centrifuge tube 2. During the transfer of the first centrifuge tube 1 and the second centrifuge tube 2, a strong external force could easily cause the straw 3 between the first centrifuge tube 1 and the second centrifuge tube 2 to break, rendering the device unusable. By inserting the two ends of the fixing ring 6 into the limiting grooves 11 at the flat ends of the first centrifuge tube 1 and the second centrifuge tube 2, the fixing ring 6 limits the movement of the first centrifuge tube 1 and the second centrifuge tube 2, preventing the straw 3 from breaking under strong external force, thereby enhancing the structural strength of the device.

[0062] It is foreseeable that when the retaining ring 6 is installed at the flat ends of the first centrifuge tube 1 and the second centrifuge tube 2, one end of the retaining ring 6 abuts against the first centrifuge tube 1, and the other end abuts against the second centrifuge tube 2. At this time, the distance between the second centrifuge tube 2 and the first centrifuge tube 1 cannot be changed, that is, the insertion depth of the straw 3 in the first centrifuge tube 1 cannot be changed. Therefore, this device is used when not in use, with the retaining ring 6 installed at the flat ends of the first centrifuge tube 1 and the second centrifuge tube 2, and the retaining ring 6 removed when in use.

[0063] In this embodiment, the fixing ring 6 is provided with a plug 61 to seal the air inlet 51. See below for specific effects. Figure 7 To prevent the cavity of the first centrifuge tube 1 from being contaminated by the external environment before use due to the air inlet 51 being connected to the outside, the fixing ring 6 is also provided with a plug 61 in this application. When the device is not in use, the fixing ring 6 is installed on the flat ends of the first centrifuge tube 1 and the second centrifuge tube 2, and the plug 61 on the fixing ring 6 seals the air inlet 51, thereby preventing the cavity of the first centrifuge tube 1 from being exposed to the external environment; when the device is in use, the fixing ring 6 is removed from the flat ends of the first centrifuge tube 1 and the second centrifuge tube 2, and the plug 61 on the fixing ring 6 no longer seals the air inlet 51.

[0064] In this embodiment, the sealing cap 4 includes a soft rubber stopper 41 that can be pierced by a needle, and a sealing aluminum cap 42 that fixes the soft rubber stopper 41 to the ends of the first centrifuge tube 1 and the second centrifuge tube 2. The sealing aluminum cap 42 exposes the middle part of the soft rubber stopper 41. For specific effects, see [link to documentation]. Figure 5 The sealing cap 4 includes a soft rubber stopper 41 and a sealing aluminum cap 42. The sealing aluminum cap 42 wraps around the soft rubber stopper 41, so that when the sealing aluminum cap 42 is fixed to the ends of the first centrifuge tube 1 and the second centrifuge tube 2, the soft rubber stopper 41 is also simultaneously fixed to the ends of the first centrifuge tube 1 and the second centrifuge tube 2. However, the sealing aluminum cap 42 does not completely cover the soft rubber stopper 41, but exposes the middle part of the soft rubber stopper 41, so that the needle of the syringe can penetrate the exposed part of the soft rubber stopper 41 and enter the first centrifuge tube 1 and the second centrifuge tube 2. Compared with the conventional sealing method of sealing the opening to be sealed by sealing with an aluminum foil sealing sheet and fixing the soft rubber stopper 41 to the ends of the first centrifuge tube 1 and the second centrifuge tube 2 by the sealing aluminum cap 42, the fixing and sealing effect is better, thereby preventing blood leakage from the first centrifuge tube 1 and the second centrifuge tube 2 and reducing the risk of medical accidents.

[0065] A method for preparing PRP, using a syringe, a centrifuge, and the aforementioned PRP preparation apparatus, includes the following steps:

[0066] S1: The collected patient blood is pierced through the cap 4 of the first centrifuge tube 1 with a syringe and delivered into the cavity of the first centrifuge tube 1;

[0067] S2: Install the first centrifuge tube 1 into the centrifuge, set the centrifugation time and centrifugation force, and then start the centrifuge.

[0068] S3: Adjust the insertion depth of the straw 3 in the first centrifuge tube 1 until the straw 3 is about 1 to 2 mm away from the dividing layer in the first centrifuge tube 1;

[0069] S4: By piercing the cap 4 of the second centrifuge tube 2 with a syringe, the air in the cavity of the second centrifuge tube 2 is extracted, so that the first centrifugation target liquid 8 in the first centrifuge tube 1 enters the cavity of the second centrifuge tube 2 through the pipette 3.

[0070] S5: After flipping the first centrifuge tube 1 and the second centrifuge tube 2, install the second centrifuge tube 2 into the centrifuge, reset the centrifugation time and centrifugation force, and start the centrifuge.

[0071] S6: Pierce the cap 4 of the second centrifuge tube 2 with a syringe to extract the second centrifugation target liquid 10 from the second centrifuge tube 2.

[0072] The above-described contents can be implemented individually or in various combinations, and these variations are all within the protection scope of this invention.

[0073] It should be noted that, in this document, relational terms such as "first" and "second" are used merely to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such a process, method, article, or apparatus. Without further limitations, an element defined by the phrase "comprising one..." does not exclude the presence of other identical elements in the process, method, article, or apparatus that includes the element.

[0074] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, and not to limit them. Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some of the technical features; and these modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A PRP preparation apparatus, characterized in that: It includes a first centrifuge tube (1), a second centrifuge tube (2), and a pipette (3) that connects the cavities of the first centrifuge tube (1) and the second centrifuge tube (2); one end of the first centrifuge tube (1) and the second centrifuge tube (2) are provided with a sealing cap (4) that can be pierced by a needle; one end of the pipette (3) is inserted into the cavity of the first centrifuge tube (1), and the other end is inserted into the cavity of the second centrifuge tube (2); the insertion depth of the pipette (3) in the first centrifuge tube (1) is adjustable; the end of the pipette (3) in the second centrifuge tube (2) is fixedly connected to the second centrifuge tube (2); The cap (4) at one end of the first centrifuge tube (1) is used to inject blood into the cavity of the first centrifuge tube (1), and the cap (4) at one end of the second centrifuge tube (2) is used to extract the first centrifugation target liquid (8) formed after the first centrifugation separation in the cavity of the first centrifuge tube (1) into the cavity of the second centrifuge tube (2) through the pipette (3), and to extract the second centrifugation target liquid (10) formed after the second centrifugation separation in the cavity of the second centrifuge tube (2).

2. The PRP preparation apparatus as described in claim 1, characterized in that: The first centrifuge tube (1) and the second centrifuge tube (2) are both conical tubes. The sealing cap (4) seals the cavity of the first centrifuge tube (1) and the second centrifuge tube (2) at the pointed end. The straw (3) connects the flat end of the first centrifuge tube (1) and the second centrifuge tube (2).

3. The PRP preparation apparatus as described in claim 2, characterized in that: The straw (3) is provided with an external thread (31), and the flat end of the first centrifuge tube (1) is provided with a threaded hole (52) that mates with the external thread (31).

4. The PRP preparation apparatus as described in claim 3, characterized in that: The first centrifuge tube (1) has an opening at the flat end that connects the cavity to the outside, and also includes a butyl rubber stopper (5) that seals the opening at the flat end of the first centrifuge tube (1). The butyl rubber stopper (5) has an air inlet (51) that connects the cavity of the first centrifuge tube (1) to the outside, and the threaded hole (52) is provided on the butyl rubber stopper (5).

5. The PRP preparation apparatus as described in claim 4, characterized in that: An air filter (53) is provided at the bottom of the air inlet (51), and the air filter (53) is used to filter the air entering the cavity of the first centrifuge tube (1) from the outside.

6. The PRP preparation apparatus as described in claim 4, characterized in that: It also includes a fixing ring (6) for limiting the flat end of the first centrifuge tube (1) and the flat end of the second centrifuge tube (2). The flat ends of the first centrifuge tube (1) and the second centrifuge tube (2) are provided with limiting grooves (11). The two ends of the fixing ring (6) are respectively inserted into the limiting grooves (11) of the first centrifuge tube (1) and the second centrifuge tube (2).

7. The PRP preparation apparatus as described in claim 6, characterized in that: The fixing ring (6) is provided with a plug (61) to seal the air inlet (51).

8. The PRP preparation apparatus as described in claim 1, characterized in that: The sealing cap (4) includes a soft rubber plug (41) that can be pierced by a needle, and a sealing aluminum cap (42) that fixes the soft rubber plug (41) to the ends of the first centrifuge tube (1) and the second centrifuge tube (2), the sealing aluminum cap (42) exposing the middle part of the soft rubber plug (41).

9. A method for preparing PRP, characterized in that: The preparation of PRP using a syringe, a centrifuge, and the PRP preparation apparatus described in claim 1 includes the following steps: S1: The collected patient blood is pierced through the cap (4) of the first centrifuge tube (1) with a syringe and transported into the cavity of the first centrifuge tube (1); S2: Install the first centrifuge tube (1) into the centrifuge, set the centrifugation time and centrifugation force, and then start the centrifuge. S3: Adjust the insertion depth of the straw (3) in the first centrifuge tube (1) until the straw (3) is 1-2 mm away from the layer in the first centrifuge tube (1); S4: By piercing the cap (4) of the second centrifuge tube (2) with a syringe, the air in the cavity of the second centrifuge tube (2) is extracted, so that the first centrifugation target liquid (8) in the first centrifuge tube (1) enters the cavity of the second centrifuge tube (2) through the pipette (3); S5: After flipping the first centrifuge tube (1) and the second centrifuge tube (2), install the second centrifuge tube (2) into the centrifuge, reset the centrifugation time and centrifugation force, and start the centrifuge. S6: Pierce the cap (4) of the second centrifuge tube (2) with a syringe and extract the second centrifugation target liquid (10) from the second centrifuge tube (2).

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