Bionic cigarette smoking and smoke collection system and application thereof

Abstract

The application discloses a bionic cigarette smoking and smoke collection system, which comprises a 3D printing bionic smoking platform and an in-situ smoke collection system; the 3D printing bionic smoking platform is an artificial lung driven elastomer material 3D printing bionic respiratory tract and lung model; and the in-situ smoke collection system is a single particle smoke sample in-situ collection based on electrostatic modification technology. The bionic cigarette smoking platform and the smoke collection method can be applied to bionic cigarette smoking test and in-situ collection of cigarette smoke particles. The bionic cigarette smoking platform is a bionic 3D printing elastomer cigarette smoking system driven by an artificial lung. The smoke collection method is an in-situ collection technology of single particle smoke samples based on electrostatic modification technology.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of new device and interface modification, in particular to a bionic cigarette smoking and smoke collection system. BACKGROUND

[0002] China is one of the largest cigarette consumption markets in the world, and there are various types of cigarettes on the market with huge annual sales. Since the particle size, concentration and chemical composition of smoke affect the sensory quality and safety of smoke, it is extremely important to study the preparation and collection methods of cigarette smoke samples and the detection methods of physical and chemical properties to explore the quality and safety of cigarettes.

[0003] In the detection process of the physical and chemical properties of smoke, the preparation and collection of smoke samples are an important link. At present, researchers mainly collect smoke samples by Cambridge filter, which collects smoke on a very narrow filter membrane, mainly for detecting the chemical composition of smoke. The smoke particles on the Cambridge filter cover each other and cannot be used to detect the particle size distribution. Therefore, researchers also use multiple dilution methods to collect smoke samples for detecting particle size. However, when the concentration of smoke is high, the particles are easy to collide, agglomerate and condense, which changes the particle size and distribution. Therefore, the smoking mode, smoke dilution and aging process during the measurement process can affect the measurement results of the particle size distribution of the smoke sample, which puts forward higher requirements for the preparation and collection of smoke samples. At present, most of the smoking test methods of cigarettes or heated cigarettes use simple Cambridge filter smoking. These methods use an airway shape that is very different from the human respiratory tract. Moreover, the temperature and humidity of the internal and external environment of these smoking test systems are uncontrollable, and they cannot simulate the exhalation process after smoking, which is quite different from the normal smoking process and cannot meet the needs of evaluating the properties of heated cigarette smoke. Therefore, it is of great significance to construct a deep bionic smoke smoking and collection system.

[0004] Based on the traditional flue gas collection method, many studies have reported cigarette smoke particle collection and detection methods. For example, the size distribution of cigarette smoke particles was measured by electrostatic migration method; the particle size distribution of electrically heated cigarette smoke was detected by a fast particle size spectrometer, and the size of cigarette smoke particles was detected. These detection methods can give a distribution of the size of the smoke particles, but they cannot directly observe the smoke particles and cannot give more information about the smoke particles. They cannot meet the needs of evaluating the physical properties, sensory properties and toxicological properties of heated cigarette smoke. The most important defect is that it cannot collect smoke samples in situ. Traditional smoke collection mainly uses liquid filtration collection, wet cotton or sponge collection, etc. The smoke samples collected by these methods are all liquid dispersion samples. During the conversion from dispersed solid smoke particles to liquid dispersion samples, the particle size distribution and physical and chemical properties of the smoke particles change, and the first-hand dispersion and particle size distribution of the smoke particles cannot be obtained. SUMMARY

[0005] The problem to be solved by the present application is that, in view of the shortcomings of the above-mentioned cigarette smoking system, namely the problem of bionic respiratory tract restoration and the problem of in-situ smoke particle collection, an innovative solution is proposed to construct a cigarette smoking platform with a bionic human respiratory tract structure and an in-situ smoke particle collection method.

[0006] To solve the above problems, the present application adopts the following scheme: a bionic cigarette smoking and smoke collection system, characterized in that it comprises a 3D printed bionic smoking platform and an in-situ smoke collection system; the 3D printed bionic smoking platform is an elastomer material 3D printed bionic respiratory tract and lung model driven by an artificial lung; the in-situ smoke collection system is a single-particle smoke sample in-situ collection based on electrostatic modification technology.

[0007] Further, the bionic cigarette smoking and smoke collection system is characterized in that the elastomer material 3D printed bionic respiratory tract and lung model has a cavity with the same shape as the human respiratory tract system, including the oral cavity, nasal cavity, organs and lungs, and is constructed in a 1:1 configuration with the human respiratory tract and lung, and the elastomer material is organic silicone elastomer and / or organic silicone gel; the elastomer material 3D printed bionic respiratory tract and lung model is provided with an artificial lung, a warm air atomization humidification device and a test-feedback system, the artificial lung is used to drive the contraction and relaxation of the bionic respiratory tract cavity, control the internal negative pressure and positive pressure; the warm air atomization humidification device and the test-feedback system are used to control the temperature and humidity inside the cavity.

[0008] Further, the bionic cigarette smoking and smoke collection system is characterized in that the connection part of the artificial lung and the warm air atomization humidification device with the elastomer material 3D printed bionic respiratory tract and lung model is provided with a HEPA filter membrane and an activated carbon filter screen.

[0009] Further, the biomimetic cigarette smoking and smoke collection system is characterized in that the in-situ smoke collection system comprises a smoke sample collection substrate arranged at the oral cavity, tongue, nasal cavity, throat and trachea of the 3D-printed biomimetic respiratory tract and lung model made of an elastomer material.

[0010] Further, the biomimetic cigarette smoking and smoke collection system is characterized in that the preparation method of the surface hydrophilic and electrostatically modified silicon substrate comprises the following steps: ultrasonic cleaning treatment of the silicon substrate in isopropyl alcohol, power 100W, cleaning time 15 minutes; after the silicon substrate is dried by blowing nitrogen, it is soaked in a chromic acid solution, and is treated in a constant temperature box at 80 DEG C for 30 minutes; the silicon substrate is taken out, washed with ultrapure water three times, and dried by blowing nitrogen; the substrate is soaked in a toluene solution containing 1% by mass fraction of 3-aminopropyl triethoxysilane (APTES) for 2 hours; the substrate is taken out, washed with toluene and ethanol three times, and stored in a dry dish at 4 DEG C for standby.

[0011] A biomimetic cigarette smoking and smoke collection system is characterized in that it is applied to cigarette and heated cigarette smoking test and smoke collection.

[0012] A biomimetic cigarette smoking and smoke collection system is characterized in that it is applied to electronic cigarette smoking test and smoke collection.

[0013] The technical effects of the present application are as follows:

[0014] 1. Compared with the traditional cigarette smoking device, the biomimetic cigarette smoking platform in the present application can simulate the overall biomimetic cigarette smoking process of the human respiratory tract system, and important positions (oral cavity, tongue, nasal cavity, throat and trachea) in the biomimetic smoking platform are reserved for installing smoke sample collection substrates, so as to collect smoke samples at different positions.

[0015] 2. Compared with the traditional cigarette smoking device, the biomimetic cigarette smoking platform in the present application can maintain the stability of the internal and external environment, and the internal and external biomimetic smoking platforms are respectively connected with a temperature and humidity controller, so as to simulate a stable human physiological environment in the internal biomimetic smoking platform and a stable and controllable temperature and humidity environment outside the biomimetic smoking platform.

[0016] 3. Compared with the traditional cigarette smoking device, the biomimetic cigarette smoking platform in the present application can control the smoking frequency and tidal volume, simulate the vital capacity of different age groups by changing the smoking volume (tidal volume), realize difference smoke volume simulation, and realize smoking simulation of people in different altitude regions by changing the internal and external pressure difference, etc.

[0017] 4. Compared with the traditional cigarette smoke collection device, the cigarette smoke collection method in the application can collect the smoke inhaled into the bionic respiratory tract in situ, avoid the re-aggregation or further chemical reaction of the adsorbed smoke particles, and ensure that the physical and chemical properties of the smoke particles remain unchanged for a long time. BRIEF DESCRIPTION OF DRAWINGS

[0018] Figure 1 Schematic diagram of the bionic smoking platform of the elastomer material driven by the artificial lung.

[0019] Figure 2 Schematic diagram of the electrostatic modification of the silicon substrate.

[0020] Figure 3 Schematic diagram of in-situ collection of single-particle smoke samples based on electrostatic modification technology.

[0021] Figure 4 Property comparison diagram of the bionic cigarette smoking and smoke collection system relative to the traditional cigarette smoking and collection system.

[0022] Figure 5 Analysis of the particle size peak values of the smoke collected by the Cambridge filter and the bionic cigarette smoking and smoke collection system through dynamic light scattering detection and multiple dynamic light scattering tests.

[0023] Figure 6 In-situ collection of smoke at different positions and atomic force microscopy characterization. DETAILED DESCRIPTION

[0024] The application will be further described in detail below with reference to the accompanying drawings.

[0025] A bionic smoking platform is constructed using 3D printing technology, and the structure is as shown in Figure 1 The application uses printable elastomers to print a cavity with the same shape as the human respiratory system, and the overall structure mainly includes the oral cavity, tongue, nasal cavity, throat, trachea, etc. The platform uses an artificial lung to replace the lungs and simulate the process of smoking one puff at a time. The tongue and throat are made of high-elasticity, low-modulus, high-molecular-weight materials to ensure flexibility and simulate the deformation and displacement of the tongue and throat during the smoking process. The smoke enters from the oral cavity and is exhaled from the oral cavity and nasal cavity, and the entire movement process is highly similar to the movement of smoke in the human respiratory system. Important positions (oral cavity, tongue, nasal cavity, throat, trachea) in the bionic smoking platform are pre-reserved for installing smoke sample collection substrates to facilitate the collection of smoke samples at different positions.

[0026] In order to keep the environment inside and outside the bionic suction platform stable, the project plans to connect the temperature and humidity controller inside and outside the bionic suction platform respectively, connect the atomizing humidifier and the hair dryer inside the bionic suction platform, and connect the negative feedback temperature and humidity controller to simulate the stable and controllable temperature and humidity environment and ensure the temperature and humidity inside the cavity.

[0027] In the bionic suction platform system, an artificial lung, i.e. a breathing machine, is used to replace the traditional suction machine Figure 1 ), which has obvious advantages in simulating the cigarette smoking of different people in different regions. The artificial lung can change the amplitude of suction (suction positive pressure and negative pressure setting), frequency (suction interval setting), humidity (cavity humidity inside), and is used for experimental simulation and smoke sample collection of different smoking habits; at the same time, the artificial lung can also simulate the vital capacity of different age groups by changing the suction volume (tidal volume), realize difference smoke suction simulation, realize different altitude population suction simulation by changing the internal and external pressure difference, etc.

[0028] Through surface hydrophilic and electrostatic modification of the silicon substrate, the substrate can attract and adsorb smoke particles Figure 2 ), so as to realize direct adsorption and collection of diffuse smoke.

[0029] The electrostatic modification method of the silicon substrate is to cut the silicon wafer substrate into 1×1cm 2 first, ultrasonic cleaning treatment in isopropyl alcohol, power 100W, cleaning time 15 minutes; after the silicon substrate is blown dry with nitrogen, it is immersed in a chromium acid solution and placed in a constant temperature oven at 80℃ for 30 minutes; the silicon substrate is washed with ultrapure water for three times and dried with nitrogen; the above-mentioned substrate is immersed in a toluene solution containing 3-aminopropyl triethoxysilane (APTES) for 2 hours, and the concentration of 3-aminopropyl triethoxysilane is 1% mass fraction; the substrate is taken out and washed with toluene and ethanol for three times, and stored in a dry dish at 4℃ for standby.

[0030] The performance of the present application is tested by the following examples.

[0031] Example 1 Comparison between the bionic cigarette smoking and smoke collection system in the present application and the traditional cigarette smoking and smoke collection system

[0032] In order to show the advantages of the bionic cigarette smoking platform and smoke collection system in the present application over the traditional cigarette smoking and collection system, this example lists the comparison of some properties of the two systems Figure 3). Traditional smoke suction collection system mainly uses Cambridge filter to collect smoke samples, which collects smoke on extremely narrow filter membrane, cannot simulate the movement of smoke in respiratory tract, and cannot collect single particle. The bionic suction platform designed in the project has the same shape as human respiratory tract, which can simulate the environment inside and outside human respiratory tract to the greatest extent. In addition to simulating simple suction process, it can also simulate the cigarette smoking process of different people and different regions in complex environment and complex state, and complete the corresponding sampling.

[0033] Example 2: Comparison of particle size distribution of smoke collected by bionic cigarette suction platform in the application and traditional smoke collection system

[0034] In order to verify the influence of bionic cigarette suction platform in the application on cigarette smoke particle collection and dispersed particles, the applicant compared the smoke collected by Cambridge filter and the smoke collected by bionic cigarette suction platform in the application, and detected the solution after dissolving the two in water by dynamic light scattering. As shown in Figure 5 A, dynamic light scattering detection showed that the dispersion range of cigarette smoke particles collected by traditional smoke collection system was 1000-8000 nm, while the dispersion range of cigarette smoke particles collected by bionic cigarette suction platform and smoke collection system in the application was 400-1600 nm; as shown in Figure 5 B, the peak value of particle size of multiple dynamic light scattering tests was analyzed, and it was found that the average particle size of cigarette smoke particles collected by traditional method was about 3.5 μm, while the average particle size of cigarette smoke particles collected by bionic cigarette suction platform in the application was about 720 nm. As can be seen, the cigarette smoke particles collected by bionic cigarette suction platform in the application have higher dispersion degree and smaller particle size, while the cigarette smoke particles collected by the traditional collection method have gathered and cannot truly show the particle size distribution of cigarette smoke particles.

[0035] Example 3: In-situ collection and morphology analysis of cigarette smoke particles in the application

[0036] In order to show the application of bionic suction platform and in-situ collection in cigarette smoke collection in the application, the applicant used bionic suction platform in the application to collect cigarette smoke in-situ at different positions in bionic respiratory tract and characterized by atomic force microscope. As shown in Figure 6 a, at different positions in respiratory tract, including oral cavity position (i), throat position (ii), middle segment of trachea (iii) and nasal cavity (iv), electrostatic modified substrate was set for cigarette smoke particle collection, and then atomic force microscope was used to detect the substrate collected cigarette smoke particles. As shown in Figure 6 b, in oral cavity position, due to high humidity of smoke, smoke aggregation is relatively concentrated, so flocculent or aggregated granular structure can be found, with height of more than 100 nm and aggregation scale of micron level; as shown inFigure 6 c shows that at the throat position, the smoke starts to disperse, and the large particles have already sunk before reaching the collection substrate, so the collected particles are relatively uniform medium smoke particles with a particle size of 10-80 nm and a high particle density; as Figure 6 d shows that at the middle of the trachea, the medium smoke particles have also dispersed, so the collected particles are small particles with a low density (mass / volume ratio) and a low particle density, and the particle diameter is below 20 nm. This embodiment details the application and advantages of the biomimetic suction platform and in-situ collection method of the present application in collecting and directly characterizing the particle size and dispersion degree of cigarette smoke particles.

Claims

1. A bionic cigarette smoking and smoke collection system, characterized in that, The application relates to a 3D printing bionic suction platform and an in-situ smoke collection system; the 3D printing bionic suction platform is an elastomer material 3D printing bionic respiratory tract and lung model driven by an artificial lung; the in-situ smoke collection system is a single-particle smoke sample in-situ collection based on electrostatic modification technology; the elastomer material 3D printing bionic respiratory tract and lung model has a cavity with the same shape as a human respiratory tract system, including a mouth cavity, a nasal cavity, organs and lungs, and is constructed according to the human respiratory tract and lung configuration 1:1, and the elastomer material is organic silicon elastomer and / or organic silicon gel; the elastomer material 3D printing bionic respiratory tract and lung model is provided with an artificial lung, a warm air atomization humidification device and a test-feedback system, the artificial lung is used for driving the bionic respiratory tract cavity to contract and dilate, and controlling the internal negative pressure and positive pressure; the warm air atomization humidification device and the test-feedback system are used for controlling the internal temperature and humidity of the cavity; the in-situ smoke collection system includes a smoke sample collection substrate arranged at the mouth cavity, the tongue, the nasal cavity, the throat and the trachea of the elastomer material 3D printing bionic respiratory tract and lung model; the smoke sample collection substrate is a surface hydrophilic and electrostatically modified silicon substrate; the preparation method of the surface hydrophilic and electrostatically modified silicon substrate comprises the following steps: ultrasonic cleaning treatment of the silicon substrate in isopropyl alcohol, power 100 W, cleaning time 15 minutes; after the silicon substrate is dried by nitrogen blowing, the silicon substrate is soaked in a chromic acid solution, and is treated in a thermostat at 80 DEG C for 30 minutes; the silicon substrate is taken out, washed with ultrapure water for three times, and dried by nitrogen blowing; the substrate is soaked in a toluene solution containing 1% mass fraction of 3-aminopropyl triethoxysilane (APTES) for 2 hours; the substrate is taken out, washed with toluene and ethanol for three times, and stored in a dry dish at 4 DEG C for standby.

2. The bionic cigarette smoking and smoke collecting system according to claim 1, characterized in that, The connection part of the artificial lung and the warm air atomization humidification device and the elastomer material 3D printing bionic respiratory tract and lung model is provided with a HEPA filter membrane and an activated carbon filter screen.

3. The bionic cigarette smoking and smoke collecting system according to claim 1, characterized in that, The application is applied to various cigarette and heating cigarette suction tests and smoke collection.

4. The bionic cigarette smoking and smoke collecting system according to claim 1, characterized in that, The application is applied to various electronic cigarette suction tests and smoke collection.

Images