Slide boxes and blood slide preparation devices

Abstract

The present application relates to the field of in-vitro diagnosis, and particularly to a slide box and a blood film making device for performing staining treatment on a slide. The slide box has a plurality of slide holding cavities for holding a slide in an upright state; the plurality of slide holding cavities include a first type of slide holding cavity for fixing a slide carrying a blood film with a fixing solution and a second type of slide holding cavity for drying the slide after the fixing treatment. With this structure, the slide can be dried immediately after the fixing treatment, thus speeding up the staining process and improving the staining efficiency.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of in vitro diagnosis, in particular to a slide box and a blood smearing device for performing staining treatment on a slide. BACKGROUND

[0002] Before staining in a slide staining machine, under certain staining conditions, methanol fixation needs to be performed on the slide carrying the blood film, that is, the cells on the slide are pre-fixed by methanol. In order to obtain better staining quality and imaging effect, it is also necessary to dry the methanol attached to the slide after methanol pre-fixing.

[0003] In the prior art, the main staining method includes box staining. Box staining completes all processes such as methanol fixation, methanol drying, and multi-step staining in a separate small container. Therefore, after completing the operation of the previous step, if the next step needs to be performed, the liquid of the previous step needs to be discharged before the next step can be performed. Therefore, if the next staining needs to be performed, the methanol needs to be discharged as waste liquid before the next staining step can be performed. The operation process is complex, and the processing efficiency is low. Moreover, methanol is a dangerous product with high volatility and flammability, and the amount of methanol provided by a hospital is strictly limited. Using methanol as a one-time use will inevitably increase the amount of methanol used, resulting in safety risks in methanol storage, and poor user experience of the hospital. SUMMARY

[0004] The purpose of the present application is to provide a slide box and a blood smearing device that can improve the staining processing efficiency.

[0005] The technical solution adopted by the present application is as follows:

[0006] A slide box comprises: a plurality of slide holding cavities capable of holding a slide in an upright state; and a plurality of the slide holding cavities, including a first type of slide holding cavity for fixing a slide carrying a blood film with a fixing liquid and a second type of slide holding cavity for drying the slide after the fixing treatment.

[0007] In one embodiment, the first type of slide holding cavity is arranged adjacent to the second type of slide holding cavity in the thickness direction of the slide held in the second type of slide holding cavity, and the first type of slide holding cavity and the second type of slide holding cavity hold the slide parallel to each other.

[0008] In one embodiment, the slide box comprises a plurality of processing assemblies, each of which comprises one second type of slide holding cavity and two first type of slide holding cavities, and the first type of slide holding cavities are arranged adjacent to both sides of the second type of slide holding cavity in the thickness direction of the slide held in the second type of slide holding cavity.

[0009] In one of the embodiments, the plurality of processing components are arranged adjacent to each other along the thickness direction of the slide held in the second type of slide holding cavity, and the plurality of processing components are integrally formed.

[0010] In one of the embodiments, the first type of slide holding cavity comprises a fixing solution cavity for storing a fixing solution and capable of holding a slide in an upright state, the top of the fixing solution cavity is provided with a first insertion opening for inserting the slide, and the bottom wall of the fixing solution cavity is provided with a first flow-through opening for supplying / discharging the fixing solution.

[0011] In one of the embodiments, the first type of slide holding cavity further comprises an overflow cavity arranged adjacent to the fixing solution cavity, the overflow cavity and the fixing solution cavity share a common side wall, and the common side wall is provided with an overflow gap for limiting the position of the liquid level of the fixing solution in the fixing solution cavity to be between the pushing area and the printing area of the slide when the slide is soaked in the fixing solution in the fixing solution cavity, and the bottom wall of the overflow cavity is provided with a second flow-through opening for discharging the fixing solution.

[0012] In one of the embodiments, the overflow cavity is located between the fixing solution cavity and the second type of slide holding cavity, and the bottom wall of the second type of slide holding cavity is provided with a third flow-through opening for discharging the fixing solution.

[0013] In one of the embodiments, the bottom of the slide box is further provided with a first pipeline and a first total flow-through opening, and the first total flow-through opening is in communication with the plurality of first flow-through openings through the first pipeline.

[0014] In one of the embodiments, the first pipeline extends along the thickness direction of the slide held in the second type of slide holding cavity and is arranged through the two side walls of the slide box, and the two ends of the first pipeline are provided with sealing plugs.

[0015] In one of the embodiments, the bottom of the slide box is further provided with a second pipeline, a third pipeline, a second total flow-through opening and a third total flow-through opening, the second total flow-through opening is in communication with the plurality of second flow-through openings through the second pipeline, the third total flow-through opening is in communication with the plurality of third flow-through openings through the third pipeline, and the first pipeline, the second pipeline and the third pipeline are arranged parallel to each other.

[0016] In one of the embodiments, the top of the second type of slide holding cavity is provided with a second insertion opening for inserting the slide, and the cavity wall of the second type of slide holding cavity is provided with a gas supply channel for supplying gas to the second type of slide holding cavity.

[0017] In one of the embodiments, the air supply channel has an air inlet arranged near the second socket and an air outlet arranged at or near the middle of the second type of slide holding cavity to supply air to the bottom of the second type of slide holding cavity.

[0018] In one of the embodiments, the air supply channel forms an angle of 5 to 20 degrees with the slide held in the second type of slide holding cavity.

[0019] In one of the embodiments, the slide held in the first type of slide holding cavity has first slide holding grooves arranged on both sides in the width direction of the slide, the first slide holding grooves extending from the top opening of the first type of slide holding cavity to the bottom of the first type of slide holding cavity along the length direction of the slide held in the first type of slide holding cavity.

[0020] and / or

[0021] In one of the embodiments, the slide held in the second type of slide holding cavity has second slide holding grooves arranged on both sides in the width direction of the slide, the second slide holding grooves extending from the top opening of the second type of slide holding cavity to the bottom of the second type of slide holding cavity along the length direction of the slide held in the second type of slide holding cavity.

[0022] In one of the embodiments, the slide box is a top-opened cuboid box.

[0023] In one of the embodiments, the first type of slide holding cavity and the second type of slide holding cavity are cuboids matching the shape of the slide.

[0024] In one of the embodiments, the cavity walls constituting the first type of slide holding cavity and the second type of slide holding cavity are integrally formed with the rest of the box body of the slide box except the cavity walls.

[0025] In one of the embodiments, the fixing solution is methanol.

[0026] A blood film making device for staining a slide carrying a blood film, comprising:

[0027] A staining tank for staining the blood film on the slide by the stored liquid, the staining tank being provided with the slide box as described above;

[0028] A slide transfer component for transferring the slide between the first type of slide holding cavity and the second type of slide holding cavity.

[0029] In one of the embodiments, the slide box is provided with an ear on each of the two side walls in the thickness direction of the slide held in the slide box, the ear is extended outward by the corresponding side wall of the slide box, and a second positioning protrusion is arranged on the ear.

[0030] In one of the embodiments, the dye vat is provided with a mounting cavity extending through the top and bottom, the mounting cavity is provided with a mounting cover plate at the top, the mounting cover plate is provided with an opening for the slide to pass through and positioning through holes on both sides of the opening matched with the second positioning protrusion, and the slide box is arranged inside the mounting cavity and fixedly connected with the mounting cover plate.

[0031] In summary, due to the adoption of the above technical solutions, the application has the following beneficial effects:

[0032] 1. In the application, the slide box includes a first slide holding cavity for fixing the slide carrying the blood film and a second slide holding cavity for drying the fixed slide, so that the slide can be dried immediately after the fixing of the slide carrying the blood film, the operation is convenient, the dyeing process of the slide is accelerated, and the dyeing efficiency of the slide is improved.

[0033] 2. In the application, the dye vat of the slide making device is provided with a slide box, the slide box includes a first slide holding cavity for fixing the slide carrying the blood film and a second slide holding cavity for drying the fixed slide, so that the slide can be dried immediately after the fixing of the slide carrying the blood film, the operation is convenient, the dyeing process of the slide is accelerated, and the dyeing efficiency of the slide is improved. BRIEF DESCRIPTION OF DRAWINGS

[0034] Figures 1A-1C is a structural schematic diagram of one embodiment of the slide involved in the application;

[0035] Figure 2 is a structural schematic diagram of one embodiment of the slide making device involved in the application;

[0036] Figure 3A and Figure 3B is a structural schematic diagram of one embodiment of the dye vat involved in the application;

[0037] Figure 4A is a top view of one embodiment of the slide making device involved in the application;

[0038] Figure 4B is a sectional view of one embodiment of the slide making device involved in the application;

[0039] Figures 5A-5C is a structural schematic diagram of one embodiment of the slide box involved in the application;

[0040] Figure 5D and Figure 5E is a cross-sectional view of an embodiment of a slide cassette with which the present application is concerned;

[0041] Figure 5F is a schematic structural view of an embodiment of a slide making device with which the present application is concerned;

[0042] Figure 5G is a cross-sectional view of an embodiment of a slide making device with which the present application is concerned;

[0043] Figure 5H and Figure 5I is a schematic structural view of an embodiment of a slide cassette with which the present application is concerned;

[0044] Figure 6 is a schematic structural view of an embodiment of a cover plate with which the present application is concerned.

[0045] Figure 7A is a schematic structural view of an embodiment of a slide making device with which the present application is concerned;

[0046] Figure 7B is a top view of an embodiment of a slide making device with which the present application is concerned;

[0047] Figure 7C is a cross-sectional view of an embodiment of a slide making device with which the present application is concerned;

[0048] Figure 8 is a schematic structural view of an embodiment of a dye vat with which the present application is concerned;

[0049] Figure 9A and Figure 9B is a schematic structural view of an embodiment of a slide cassette with which the present application is concerned;

[0050] Figure 9C is a top view of an embodiment of a slide cassette with which the present application is concerned;

[0051] Figure 9D is a side view of an embodiment of a slide cassette with which the present application is concerned

[0052] Figure 9E is Figure 9D a cross-sectional view in the direction of A-A in

[0053] Figure 9F is a front view of an embodiment of a slide cassette with which the present application is concerned;

[0054] Figure 9G is a cross-sectional view of an embodiment of a slide cassette with which the present application is concerned; DETAILED DESCRIPTION

[0055] In order to make the objects, technical solutions and advantages of the present application clearer, the slide manufacturing device of the present application will be further described in detail below with reference to the embodiments and in conjunction with the drawings. It should be understood that the specific embodiments described herein are only used to explain the present application and not to limit the present application.

[0056] The serial numbers of components in this paper, such as "first", "second", etc., are only used to distinguish the described objects and do not have any sequence or technical meaning. The "connection" and "coupling" in this application include direct and indirect connection (coupling) unless otherwise specified. In the description of the present application, it should be understood that the orientation or positional relationship indicated by the terms "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", etc. is based on the orientation or positional relationship shown in the drawings, and is only for the convenience of describing the present application and simplifying the description, and therefore cannot be understood as limiting the orientation or positional relationship of the indicated mechanism or element. It must be constructed and operated in a particular orientation, and therefore cannot be understood as limiting the present application.

[0057] In the present application, unless otherwise explicitly specified and limited, the first feature is "on" or "under" the second feature, which can be direct contact between the first and second features, or indirect contact between the first and second features through an intermediate medium. Moreover, the first feature "above", "above" and "above" the second feature can be directly above or obliquely above the first feature, or only indicate that the horizontal height of the first feature is higher than that of the second feature. The first feature "below", "below" and "below" the second feature can be directly below or obliquely below the first feature, or only indicate that the horizontal height of the first feature is less than that of the second feature.

[0058] Reference Figures 1A-1C The processing object of the blood film manufacturing device involved in the present application is described. The blood film manufacturing device is used to perform staining processing on a slide S carrying a blood film, as shown in Figures 1A-1C The slide S is in a sheet shape, with a width direction S1, a thickness direction S2 and a height direction S3. The working surface of the slide S includes a push piece area AR1 and a printing area AR2. The sample push piece processing is performed in the push piece area AR1, and the blood sample is applied in the form of a blood film on the push piece area AR1. The printing processing is performed in the printing area AR2, and the sample information is printed on the printing area AR2, which is convenient for the user to identify and read the information of the sample applied on the slide. Generally, the slide S is in a vertical state (the printing area AR2 is on the top and the push piece area AR1 is on the bottom) for staining processing, and the staining liquid is soaked in the push piece area AR1 of the slide.

[0059] Embodiment 1

[0060] Reference is made to Figures 2-6The blood film device according to the embodiment 1 is described.

[0061] The staining process generally includes a methanol fixation process, a staining solution staining process, and a cleaning solution cleaning process. As shown in Figure 3A and Figure 3B The blood film device according to the embodiment includes a staining vat 100, which performs a staining process on a blood film on a slide through a plurality of liquids stored therein. The staining vat 100 includes a fixing liquid tank 101 for storing a fixing liquid (e.g., methanol) that can fix the blood film on the slide, a staining liquid tank 102 for storing a staining liquid that can stain the blood film on the slide, and a cleaning tank 103 for accommodating a cleaning box that can clean the stained slide. By keeping the slide carrying the blood film in the fixing liquid tank 101, the staining liquid tank 102, and the cleaning tank 103 for a certain period of time, respectively, the staining process on the slide can be completed.

[0062] As shown in Figure 4A and Figure 4B The slide box 200 can be accommodated in the fixing liquid tank 101, and the slide box 200 is a rectangular box with an open top, which is accommodated in the fixing liquid tank 101 with the open top upward. The liquid level of the fixing liquid (methanol) stored in the fixing liquid tank 101 does not exceed the top opening of the slide box 200 accommodated in the fixing liquid tank 101.

[0063] As shown in Figures 5A-5I The cavity surrounded by the circumferential side wall and the bottom wall of the slide box 200 is divided into a plurality of (e.g., 6 / 7 / 8 / 9) slide holding cavities 210 by a plurality of partitions. The cavity shape of the slide holding cavity 210 is a rectangular box that matches the shape of the slide S. Each slide holding cavity 210 can hold a slide S in a self-standing state. The slide S can be inserted into the slide holding cavity 210 through the insertion hole at the top of the slide holding cavity 210.

[0064] Among the plurality of slide holding cavities 210, there are two types of slide holding cavities. One type is the first type of slide holding cavity 211 in fluid communication with the fixing liquid tank 101, i.e., the fixing liquid (methanol) stored in the fixing liquid tank 101 can enter the first type of slide holding cavity 211 and soak the slide held in the first type of slide holding cavity 211. The other type is the second type of slide holding cavity 212 in fluid isolation from the fixing liquid tank 101, i.e., the fixing liquid (methanol) stored in the fixing liquid tank 101 cannot enter the second type of slide holding cavity 212, and the second type of slide holding cavity 212 is used to perform a drying process on the slide soaked in methanol.

[0065] The blood slice preparation device of the embodiment further comprises a slice transfer component (not shown in the figure) for transferring the slices between the first slice holding cavity 211 and the second slice holding cavity 212.

[0066] The blood slice preparation device of the embodiment can reuse the fixing solution by using the fixing solution stored in the fixing solution tank to perform fixing treatment on the plurality of slices, and can accelerate the drying speed of the fixing solution on the slices, accelerate the dyeing process of the slices, and improve the dyeing treatment efficiency of the slices by transferring the slices from the first slice holding cavity to the second slice holding cavity immediately after the slices are soaked in the fixing solution, and performing drying treatment on the slices rapidly.

[0067] As shown in Figure 5B , the top of the first slice holding cavity 211 has a first insertion opening 2111 for inserting the slices, and the side wall or bottom wall of the first slice holding cavity 211 is provided with a first flow-through opening 2112 in fluid communication with the fixing solution tank 101. The fixing solution (methanol) stored in the fixing solution tank 101 enters the first slice holding cavity 211 through the first flow-through opening 2112, thereby soaking the slices S held in the first slice holding cavity 211. In order to increase the speed of the fixing solution (methanol) entering the first slice holding cavity 211, the first flow-through opening 2112 can be provided on the side wall and the bottom of the first slice holding cavity 211.

[0068] As shown in Figure 5C , the top of the second slice holding cavity 212 has a second insertion opening 212-1 for inserting the slices, and the cavity wall of the second slice holding cavity 212 is provided with a gas supply channel 213 for supplying gas to the second slice holding cavity. Dry gas can be supplied to the second slice holding cavity 212 through the gas supply channel 213 to dry the slices S held in the second slice holding cavity 212, so that the fixing solution (methanol) evaporates. Specifically, a gas supply needle connected to a gas source can be inserted into the gas supply channel 213 to supply dry air to the second slice holding cavity 212.

[0069] Preferably, as shown in Figure 5D , the gas inlet 2131 of the gas supply channel 213 is arranged near the second insertion opening 212-1, and the gas outlet 2132 of the gas supply channel is arranged at or near the middle of the second slice holding cavity 212 to supply gas to the bottom of the second slice holding cavity. As shown in Figure 5EAs shown, the air flow flows along the direction of the dotted arrow, after being blown out from the air outlet 2132, it blows to the bottom of the second-type slide holding cavity 212, rotates and gathers at the bottom, and changes direction under the action of the bottom wall, blows to the other side wall of the second-type slide holding cavity 212 (opposite to the side wall where the air supply channel 213 is arranged), continues to change direction under the action of the other side wall, and blows to the side wall of the second-type slide holding cavity 212 where the air supply channel 213 is arranged, thereby forming air backflow in the second-type slide holding cavity 212. In this way of blowing air to the bottom of the second-type slide holding cavity 212, the dry air will rotate and gather at the bottom of the second-type slide holding cavity 212, and then be discharged from the second insertion port 212-1. Because a small amount of methanol solution will gather at the bottom of the slide after the completion of methanol fixation of the slide, the air supply channel 213 arranged to blow air to the bottom of the second-type slide holding cavity 212 can accelerate the volatilization speed of the methanol solution, and sufficiently and quickly dry the slide.

[0070] Preferably, the air supply channel 213 is arranged on the side wall in the width direction of the slide held in the second-type slide holding cavity 212, and the air supply channel 213 forms an angle of 5 to 20 degrees with the slide held in the second-type slide holding cavity 212, that is, the air supply channel 213 forms an angle of 5 to 20 degrees with the slide in the direction opposite to the side wall of the air supply channel 213. In this way, as shown in Figure 5E , the air backflow can cover the entire slide pushing area AR1 of the slide S, and the slide can be sufficiently dried.

[0071] Specifically, as shown in Figure 5F , the first-type slide holding cavity 211 is arranged adjacent to the second-type slide holding cavity 212 in the thickness direction of the slide held in the second-type slide holding cavity 212, and the first-type slide holding cavity 211 is parallel to the slide S held in the second-type slide holding cavity 212. In this way, the slides held in the first-type slide holding cavity 211 and the second-type slide holding cavity 212 are arranged in parallel along the thickness direction of the slides, the structure of the slide box is more compact, the volume of the slide box is reduced, and the distance between the slides held in the adjacent first-type slide holding cavity 211 and the second-type slide holding cavity 212 is short, which can reduce the transfer path of the slide transfer member and improve the transfer efficiency of the slide.

[0072] Specifically, as shown in Figure 5G , two side walls in the width direction of the slide held in the first-type slide holding cavity 211 are respectively provided with first slide placing grooves 2113 for holding the slide, and the first slide placing grooves 2113 extend downward along the length direction of the slide held in the first-type slide holding cavity 211 to the bottom of the first-type slide holding cavity 211. The first slide placing grooves 2113 can extend from the top to the bottom of the first-type slide holding cavity 211, or as shown inFigure 5G As shown, the middle and lower part of the first type slide holding cavity 211 extends to the bottom. The slide is inserted into the first type slide holding cavity 211 from the first insertion port 2111 of the first type slide holding cavity 211, and after reaching the first slide holding groove 2113, the two sides of the slide in the width direction enter the first slide holding groove 2113, the inserted slide is guided by the first slide holding groove 2113, and the slide is held after the insertion is completed. The first slide holding groove 2113 of this structure can reduce the contact area of the slide box and the slide, thereby reducing the dead angle / gap that can retain the fixing liquid.

[0073] Specifically, as shown in Figure 5G As shown, the two side walls of the slide in the width direction are respectively provided with a second slide holding groove 212-2 for holding the slide, and the second slide holding groove 212-2 extends along the length direction of the slide held in the second type slide holding cavity 212 from the top opening of the second type slide holding cavity 212 to the bottom of the second type slide holding cavity 212. During the process of inserting the slide into the second type slide holding cavity 212, from entering the second insertion port 212-1, the two sides of the slide in the width direction enter the second slide holding groove 212-2, and then enter the second type slide holding cavity 212 along the second slide holding groove 212-2, and after the insertion is completed, the second slide holding groove 212-2 holds the two sides of the slide in the width direction to hold the entire slide. The second slide holding groove 212-2 which holds the slide from the top to the bottom of the second type slide holding cavity 212 can keep the stability of the slide, so that the slide does not shake when dry air is blown into the second type slide holding cavity 212, thereby ensuring the uniformity of the slide drying. And because the second slide holding groove 212-2 only contacts the small area of the side of the slide in the width direction, it can minimize the influence of the second slide holding groove 212-2 on the slide drying.

[0074] As shown in Figure 5HAs shown, the slide box 200 includes a plurality of processing components 201, each processing component 201 includes a second-type slide holding cavity 212 and two first-type slide holding cavities 211, and the first-type slide holding cavity 211 is arranged adjacent to both sides of the second-type slide holding cavity 212 in the thickness direction of the slides held in the second-type slide holding cavity 212. Generally, when glass slides are fixed with methanol, the time spent soaking in methanol is longer than the time spent drying the methanol. Therefore, in this embodiment, two first-type glass slide holding cavities 211 share one second-type glass slide holding cavity 212, which can improve the utilization rate of the second-type glass slide holding cavity 212 and make the structural design of the entire glass slide box more reasonable. In addition, the two first-type glass slide holding cavities 211 are respectively located on both sides of the second-type glass slide holding cavity 212, which can minimize the stroke of the glass slide transfer component in transferring the glass slides and improve the efficiency of glass slide transfer. Furthermore, there are no other glass slides below the path of the glass slide transfer, which can prevent liquid from dripping onto the glass slide being transferred during the glass slide transfer, thereby preventing contamination of the printing area AR2 of the glass slide.

[0075] Preferably, Figure 2 and Figure 5H As shown, a plurality of processing components 201 are arranged adjacent to each other along the thickness direction of the glass slide held in the second type glass slide holding chamber, and the plurality of processing components are integrally formed. Figure 5G As shown, due to the adjacent arrangement of the processing components 210, the upper portions of the two adjacent first-type slide holding chambers 211 share a cavity, and the lower portions of the two adjacent first-type slide holding chambers 211 are each provided with a first slide placement groove 2113 for holding a slide. This simplifies the structure of the two adjacent first-type slide holding chambers 211, reducing the difficulty of manufacturing the entire slide box.

[0076] Specifically, the cavity walls constituting the first type slide holding cavity 211 and the second type slide holding cavity 212 are integrally formed with the rest of the slide box except the cavity walls. That is, the entire slide box 200 is integrally formed.

[0077] Specifically, such as Figure 5BAs shown, the second-type slide holding chamber 212 includes a first cavity 2121, a second cavity 2122, and a third cavity 2123, arranged sequentially along the thickness direction of the slide held therein. The second cavity 2122 is used to hold the slide. The bottoms of the first cavity 2121 and the third cavity 2123 are connected to the bottom of the second cavity 2122, and the tops of the first cavity 2121 and the third cavity 2123 are both open. Thus, the bottom of the second cavity 2122 can communicate with the outside world through the top openings of the first cavity 2121 and the third cavity 2123. When drying gas is blown into the second cavity 2122 from an oblique downward direction, it reaches the bottom wall of the second cavity 2122 and is discharged through the first cavity 2121 and the third cavity 2123, which are connected to the second cavity 2122. This increases the air flow rate entering the second cavity 2122, and the air flow channel formed can accelerate the drying efficiency of the slides.

[0078] Specifically, in order to facilitate installation, such as Figure 5I As shown, ears 216 for cooperating with the dye vat 100 are provided on the two side walls of the slide box 200 in the thickness direction of the slides held therein. The ears 216 are formed by extending outwards from the corresponding side walls of the slide box. Figure 4B As shown, support columns 101-3 that cooperate with the ears 216 of the slide box are provided at appropriate positions of the fixing liquid tank 101. During installation, the slide box 200 is placed in the fixing liquid tank 101 so that the ears 216 of the slide box 200 rest on the support columns 101-3, completing the initial positioning of the slide box 200.

[0079] like Figure 4B As shown, the notch of the fixed liquid tank 101 is covered by a cover plate 300 that matches the notch of the fixed liquid tank 101. By properly setting the height of the support column 101-3, the cover plate 300 covers the notch of the fixed liquid tank 101 while pressing the ear portion 216 of the slide box 200, thereby completing the vertical positioning of the slide box 200. The cover plate 300 is provided with a through hole 301 that matches the contour of the top opening of the processing assembly 201.

[0080] Specifically, such as Figure 3B As shown, the fixed liquid tank 101 includes a deep portion 101-1 and shallow portions 101-2 located on both sides of the deep portion 101-1, which correspond to the ears 216 of the slide box 200. The depth of the deep portion 101-1 is greater than the depth of the shallow portion 101-2. In this embodiment, the bottom of the deep portion 101-1 is located at a lower level than the bottom of the shallow portion 101-2, but the top opening of the deep portion 101-1 and the top opening of the shallow portion 101-2 are located at the same level. A support column 101-3 for supporting the ears 216 of the slide box 200 is provided on each of the two shallow portions 101-2. Figure 4BAs shown, the body of the slide box 200 is placed in the deep portion 101 - 1 , and the ears 216 of the slide box 200 are placed on the support columns 101 - 3 , and the slide box 200 is suspended in the fixed liquid tank 101 through the support columns 101 - 3 .

[0081] When injecting fixative solution into the fixative solution tank 101, the liquid level of the fixative solution is made to exceed the bottom wall of the shallow portion 101-2 but not exceed the printing area of ​​the glass slide placed in the fixative solution tank 101, so that the liquid level covers the entire fixative solution tank 101. The fixative solution tank 101 of this structure concentrates most of the fixative solution in the deep portion 101-1. Since the cross-sectional area of ​​the deep portion 101-1 matches the cross-sectional area of ​​the slide box 200, the cross-sectional area is small, which can reduce the amount of fixative solution used. In addition, since the liquid level of the fixative solution has reached the shallow portion 101-2, the liquid surface area of ​​the fixative solution is large. When the glass slide S is inserted into the fixative solution tank 101, the change in the height of the fixative solution is less affected.

[0082] Preferably, a plurality of first positioning protrusions 214 extending upward from the sidewalls of the slide box 200 are provided at the top opening of the slide box 200. The first positioning protrusions 214 are located on the sidewalls in the width direction of the slides held in the second-type slide holding cavity 212. Corresponding notches 302 are provided on the cover plate 300 to mate with the first positioning protrusions 214. Preferably, a first positioning protrusion 214 is provided on each side in the width direction of the slides held in the second-type slide holding cavity 212. The cooperation between the notches 302 on the cover plate 300 and the first positioning protrusions 214 on the slide box 200 enables precise horizontal positioning of the slide box 200.

[0083] The outer peripheral wall of the slide box 200 is provided with ribs 215 that abut against the inner wall of the fixing liquid tank 101 . The ribs 215 extend from the top to the bottom of the slide box 200 .

[0084] Preferably, the length of the rib 215 is less than 1 / 2 of the height of the slide box, which can reduce the contact area between the rib 215 and the fixed liquid tank 101, thereby reducing the gap where liquid may remain.

[0085] Preferably, the rib includes a tapered portion 215 - 1 , the thickness of which gradually decreases from top to bottom. The tapered portion 215 - 1 can serve as a guide when the slide box 200 is placed in the fixing liquid tank 101 .

[0086] like Figures 5A-5C As shown, it is a slide box involved in the second aspect of the present application. The slide box 200 is used to cooperate with the fixing liquid tank 101 of the dye vat 100 to achieve the fixation of the blood film carried on the slide.

[0087] The slide box 200 of the embodiment has a plurality of slide holding cavities 210, each of which can hold a slide in an upright state; the plurality of slide holding cavities 210 include: (1) a first type of slide holding cavity 211 having a top portion with a socket for slide insertion, a periphery, and / or a bottom portion with a flow-through port 2112 for communication with the outside, and (2) a second type of slide holding cavity 212 having a top portion with a socket for slide insertion, a periphery, and a closed bottom portion. The first type of slide holding cavity 211 is used to perform a fixative soaking process on a slide held therein, and the second type of slide holding cavity 212 is used to perform a drying process on a slide held therein.

[0088] The slide box 200 of this structure concentrates the second type of slide holding cavity used to perform a drying process on a slide soaked with a fixative and the first type of slide holding cavity used to perform a fixative soaking process on a slide into the same box body, which can be placed in the same fixative tank, and immediately after soaking a slide with a fixative, a drying process can be performed on the slide, which accelerates the drying speed of the fixative on the slide, speeds up the staining process of the slide, and improves the staining efficiency of the slide.

[0089] The remaining structure of the slide box 200 is the same as that of the slide making device embodiment, and will not be described here.

[0090] Embodiment 2

[0091] The following will be described with reference to Figures 7A-9E The blood slide making device related to Embodiment 2 will be described.

[0092] A blood slide making device for performing a staining process on a slide carrying a blood film, as shown in Figure 7A and Figure 8 The blood slide making device includes:

[0093] A staining vat 500 for performing a staining process on a blood film on the slide by a stored liquid, the staining vat 500 being provided with a slide box 600, as shown in Figure 9A The slide box 600 has a plurality of slide holding cavities 610 that can hold a slide S in an upright state, and the plurality of slide holding cavities include a first type of slide holding cavity 611 for performing a fixation process on a slide S carrying a blood film using a fixative (e.g., methanol) and a second type of slide holding cavity 612 for performing a drying process on the slide S after the fixation process. With this structure, a drying process can be immediately performed on the slide after the fixation process on the slide carrying the blood film, which improves the subsequent staining quality and imaging effect, speeds up the staining process of the slide, and improves the staining efficiency of the slide. Moreover, the fixation liquid (methanol) process, fixation liquid drying process, and staining liquid staining process of the slide can be simultaneously performed, which allows the fixation liquid (methanol) to be reused and reduces the amount of fixation liquid used.

[0094] Specifically, as shown in 9A, ears 619 are provided on both side walls of the slide box 600 in the thickness direction of the slide held in the slide box 600. The ears 619 are extended outward from the corresponding side walls of the slide box 600, and a second positioning protrusion 6191 is provided on the ears 619.

[0095] Specifically, such as Figures 7A-7C As shown, the dye vat 500 has a through-and-through mounting cavity 501. A mounting cover 5011 is located at the top of the mounting cavity 501. The mounting cover 5011 has an opening 5012 for a glass slide to pass through, and positioning holes on either side of the opening 5012 that mate with the second positioning protrusions 6191. The glass slide box 600 is disposed within the mounting cavity 501 and is fixedly connected to the mounting cover 5011. The second positioning protrusions 6191 mate with the positioning holes, thereby achieving horizontal positioning of the glass slide box 600. Glass slides S can be inserted through the opening in the mounting cover 5011 into the first and second glass slide holding cavities 611, 612 of the glass slide box 600. Specifically, the ears 619 on either side of the glass slide box 600 are fixedly connected to the mounting cover 5011 via screws.

[0096] Specifically, such as Figure 8 As shown, the dye vat 500 also includes a dyeing liquid tank 502 and a cleaning tank 503. The dyeing liquid tank 502 is used to store the dyeing liquid used to dye the blood film on the slide, and the cleaning tank 503 is used to accommodate a cleaning box for cleaning the stained slide. By keeping the slide bearing the blood film in the slide box 600, the dyeing liquid tank 502, and the cleaning tank 503 for a certain period of time, the slide can be stained.

[0097] The blood slide preparation device further includes a slide transfer component (not shown in the figure) for transferring slides between the first type slide holding chamber 611 and the second type slide holding chamber 612 .

[0098] Preferably, Figures 9A-9C As shown, the first type of slide holding cavity 611 is arranged adjacent to the second type of slide holding cavity 612 in the thickness direction of the slide held in the second type of slide holding cavity 612, as shown in FIG. Figure 9FAs shown, the first type of slide holding cavity 611 and the slide held in the second type of slide holding cavity 612 are parallel to each other. With this structure, the slides in the first type of slide holding cavity 611 and the second type of slide holding cavity 612 are arranged in parallel along the thickness direction of the slides, so that the structure of the entire slide cassette is more compact, the volume of the slide cassette is reduced, the distance between the slides held in the adjacent first type of slide holding cavity 611 and the second type of slide holding cavity 612 is closer, the transfer path of the slide transfer component can be reduced, and the slide transfer efficiency is improved.

[0099] Preferably, the slide cassette 600 comprises a plurality of processing assemblies, each of which comprises one second type of slide holding cavity 612 and two first type of slide holding cavities 611, which are arranged adjacent to both sides of the second type of slide holding cavity 612 in the thickness direction of the slide held in the second type of slide holding cavity 612. Generally, when the slide is fixed with methanol, the soaking time in methanol is longer than the dry methanol time, so in this embodiment, two first type of slide holding cavities 611 share one second type of slide holding cavity 612, which can improve the utilization rate of the second type of slide holding cavity 612 and make the structure design of the entire slide cassette more reasonable; and the two first type of slide holding cavities 611 are respectively located on both sides of the second type of slide holding cavity 612, which can minimize the stroke of the slide transfer component when transferring the slide and improve the slide transfer efficiency; furthermore, there is no other slide below the path of transferring the slide, which can prevent liquid from dripping onto the slide being crossed when the slide is being transferred, thereby contaminating the printing area AR2 of the slide.

[0100] Preferably, a plurality of processing assemblies are arranged adjacent to each other along the thickness direction of the slide held in the second type of slide holding cavity 612, and the plurality of processing assemblies are integrally formed.

[0101] Preferably, the first type of slide holding cavity 611 includes a fixing liquid cavity 6111 for storing a fixing liquid (methanol) and capable of holding a glass slide in an upright state. The top of the fixing liquid cavity 6111 has a first socket for inserting the glass slide, and the bottom wall of the fixing liquid cavity 6111 is provided with a first flow port 6114 for supplying / discharging the fixing liquid (methanol). With this structure, the fixing liquid can be directly supplied to the inside of the fixing liquid cavity 6111, greatly reducing the use of the fixing liquid and improving the utilization rate of the fixing liquid. When the blood preparation device is in a continuous working state, the fixing liquid cavity 6111 in the glass slide box 600 is replenished with fixing liquid according to a predetermined rehydration strategy; when the blood preparation device is in a dormant state, the fixing liquid in the fixing liquid cavity 6111 will be recycled and stored, waiting to be replenished to the fixing liquid cavity 6111 the next time the device is awakened. With the above structure, it is convenient to use a low-cost, small-scale syringe to recycle the fixing liquid, reduce the use of pumps, save internal space of the blood preparation device, and simplify the structure.

[0102] Preferably, the first-type slide holding chamber 611 also includes an overflow chamber 6115 adjacent to the fixative chamber 6111. The overflow chamber 6115 and the fixative chamber 6111 share a sidewall with an overflow notch 6116. When the slide is immersed in the fixative solution in the fixative chamber 6111, the overflow notch 6116 serves to limit the level of the fixative solution in the fixative chamber 6111 to a position between the slide's slide-pushing area AR1 and the slide's printing area AR2. This ensures that the fixative solution completely immerses the slide's slide-pushing area AR1 while preventing the fixative solution level from rising too high and contaminating the slide's printing area AR2. Furthermore, each fixative chamber 6111 is paired with one overflow chamber 6115, preventing a device malfunction from causing one overflow chamber 6115 to overflow the fixative solution from both chambers 6111. A second flow port 6117 for discharging the fixative solution is provided on the bottom wall of the overflow chamber 6115.

[0103] Preferably, the overflow chamber 6115 is located between the fixative liquid chamber 6111 and the second-type slide holding chamber 612, separating the fixative liquid chamber 6111 from the second-type slide holding chamber 612. This prevents the fixative liquid in the fixative liquid chamber 6111 from overflowing into the second-type slide holding chamber 612, thereby affecting the slides undergoing drying. The bottom wall of the second-type slide holding chamber 612 is provided with a third flow port 6123 for discharging the fixative liquid.

[0104] Preferably, Figure 9D and 9EAs shown, the bottom of the slide box 600 is further provided with a first pipe 613 and a first total flow-through port 616, and the first total flow-through port 616 is in communication with a plurality of first flow-through ports 6114 through the first pipe 613. With this structure, the number of liquid channel pipes at the bottom of the slide box 600 can be reduced, and the structure is simplified.

[0105] Preferably, the first pipe 613 extends along the thickness direction of the slide retained in the second type of slide retaining cavity 612 and is arranged through the two side walls of the slide box 600, and the two ends of the first pipe 613 are provided with sealing plugs. With this structure, the machining precision of the first pipe 613 can be ensured, the shapes and sizes of the plurality of first flow-through ports 6114 in communication with the first pipe 613 are consistent, the rising speed of the liquid level of the fixing solution in the first flow-through port 6114 corresponding fixed liquid cavity 6111 during liquid injection is consistent, and the subsequent slide fixing process is ensured. If the shapes and sizes of the plurality of first flow-through ports 6114 in communication with the first pipe 613 are inconsistent, when liquid is injected into each fixed liquid cavity 6111 through the plurality of first flow-through ports 6114, the rising speed of the liquid level of the fixing solution in each fixed liquid cavity 6111 will be inconsistent. The fixed liquid cavity 6111 with a faster rising liquid level of the fixing solution will have an overflow phenomenon, resulting in an increase in the amount of fixing solution, and after the liquid injection is completed, the fixing solution in the fixed liquid cavity 6111 with a high liquid level will flow to the fixed liquid cavity 6111 with a low liquid level under the action of atmospheric pressure to compensate and balance the fixing solution. After compensation and balance, the fixing solution in each fixed liquid cavity 6111 may not be able to completely soak the slide push area AR1, affecting the fixing process of the slide.

[0106] Preferably, the bottom of the slide box 600 is further provided with a second pipe 614, a third pipe 615, a second total flow-through port 617, and a third total flow-through port 618, the second total flow-through port 617 is in communication with a plurality of second flow-through ports 6117 through the second pipe 614, and the third total flow-through port 618 is in communication with a plurality of third flow-through ports 6123 through the third pipe 615. With this structure, the number of liquid channel pipes at the bottom of the slide box 600 can be further reduced, and the structure is simplified. The first pipe 613, the second pipe 614, and the third pipe 615 are arranged in parallel with each other, and the first pipe 613, the second pipe 614, and the third pipe 615 are not in communication with each other, eliminating the influence between each pipe.

[0107] Preferably, the second pipe 614 and the third pipe 615 both extend along the thickness direction of the slide retained in the second type of slide retaining cavity 612 and are arranged through the two side walls of the slide box 600, and the two ends of the second pipe 614 and the third pipe 615 are both provided with sealing plugs.

[0108] Preferably, as Figure 9F and 9GAs shown, the top of the second-type slide holding chamber 612 has a second insertion port for inserting the slide. A gas supply channel 6124 is provided on the wall of the second-type slide holding chamber 612 for supplying air to the second-type slide holding chamber 612. Dry gas can be supplied to the second-type slide holding chamber 612 through the gas supply channel 6124 to dry the slide S held therein and evaporate the fixative (methanol). Specifically, a gas supply needle connected to a gas source can be inserted into the gas supply channel 6124 to supply dry air to the second-type slide holding chamber 612.

[0109] Preferably, the air inlet 6125 of the air supply channel 6124 is arranged near the second socket, and the air outlet 6126 of the air supply channel 6124 is arranged in the middle of the second type glass slide holding cavity 612 or near the middle of the second type glass slide holding cavity 612 to supply air toward the bottom of the second type glass slide holding cavity 612.

[0110] Preferably, the air supply channel 6124 is disposed on a sidewall in the width direction of the slide held in the second-type slide holding cavity 612, and the air supply channel 6124 forms an angle of 5 to 20 degrees with the slide held in the second-type slide holding cavity 612. The air supply channel structure of this embodiment is consistent with that of Example 1, and its air flow direction and technical effects are consistent with those of Example 1, and are not further described here.

[0111] Preferably, Figures 7A-7C As shown, first slide receiving grooves 6113 for holding the slide are provided on both sidewalls in the width direction of the slide held in the first slide holding cavity 611. The first slide receiving grooves 6113 extend along the length of the slide held in the first slide holding cavity 611, from the top opening of the first slide holding cavity 611 to the bottom of the first slide holding cavity 611. During the insertion of a slide into the first slide holding cavity 611, both sides of the slide in the width direction enter the first slide receiving grooves 6113, beginning from the first insertion opening. The slide then descends along the first slide receiving grooves 6113 into the first slide holding cavity 611. After insertion, the first slide receiving grooves 6113 hold both sides of the slide in the width direction, thereby retaining the entire slide. This structure of the first slide receiving grooves 6113 reduces the contact area between the slide case and the slide, thereby reducing dead corners and gaps where fixative can remain.

[0112] Preferably, two side walls of the slide kept in the second type slide holding cavity 612 in the width direction of the slide are respectively provided with a second slide placing groove 6122 for holding the slide, and the second slide placing groove 6122 extends from the top opening of the second type slide holding cavity 612 to the bottom of the second type slide holding cavity 612 along the length direction of the slide kept in the second type slide holding cavity 612. During the process of inserting the slide into the second type slide holding cavity 612, from the moment of entering the second port, the two sides in the width direction of the slide enter the second slide placing groove 6122, and then enter the second type slide holding cavity 612 along the second slide placing groove 6122, and after the insertion is completed, the second slide placing groove 6122 holds the two sides in the width direction of the slide so as to hold the entire slide. The second slide placing groove 6122 which holds the slide from the top to the bottom of the second type slide holding cavity 612 can keep the stability of the slide, so that the slide does not shake when dry air is blown into the second type slide holding cavity 612, thereby ensuring the uniformity of the slide drying. Moreover, since the second slide placing groove 6122 only contacts the small area of the side in the width direction of the slide, the influence of the second slide placing groove 6122 on the slide drying can be minimized.

[0113] Preferably, the slide box 600 is a top-opened cuboid box.

[0114] Preferably, the first type slide holding cavity 611 and the second type slide holding cavity 612 are cuboids matched with the shape of the slide.

[0115] Preferably, the cavity walls constituting the first type slide holding cavity 611 and the second type slide holding cavity 612 are integrally formed with the remaining part of the box body of the slide box 600 except the cavity walls.

[0116] Through experimental comparison, compared with example 1, the slide box of the present example can reduce the use amount of fixing solution by at least 1 / 2, the use amount of fixing solution is greatly reduced, the safety risk of fixing solution storage can be effectively reduced, and the user experience of the hospital can be improved.

[0117] The above is only a specific embodiment of the present application, but the protection scope of the present application is not limited thereto, and any person skilled in the art can easily think of various equivalent modifications or replacements within the technical range disclosed by the present application, and these modifications or replacements should be covered within the protection scope of the present application. Therefore, the protection scope of the present application should be subject to the protection scope of the claims.

Claims

1. A blood slicing device, characterized in that: Including dye vats, The dye vat is provided with a dyeing liquid tank and a mounting cavity which is passed through from top to bottom. The dyeing liquid tank is used to store the dyeing liquid for dyeing the glass slide carrying the blood film. The mounting cavity is provided with a glass slide box inside. The slide box has a plurality of slide holding cavities capable of holding a slide in an upright state; The plurality of slide holding chambers include a first type of slide holding chamber for fixing a slide carrying a blood film with a fixative and a second type of slide holding chamber for drying the fixed slide; The first type of glass slide holding chamber includes a fixing liquid chamber for storing fixing liquid and capable of keeping a glass slide immersed in an upright state. The top of the fixing liquid chamber has a first socket for inserting the glass slide, and the bottom wall of the fixing liquid chamber is provided with a first flow port for supplying / discharging the fixing liquid.

2. The blood slicing device according to claim 1, characterized in that: The first type of slide holding cavity is adjacent to the second type of slide holding cavity in the thickness direction of the slide held in the second type of slide holding cavity, and the slides held in the first type of slide holding cavity and the second type of slide holding cavity are parallel to each other.

3. The blood slicing device according to claim 1, characterized in that: The slide box comprises: A plurality of processing components, each of the processing components includes one second-type slide holding cavity and two first-type slide holding cavities, and the first-type slide holding cavities are arranged adjacent to both sides of the second-type slide holding cavity in the thickness direction of the slide held in the second-type slide holding cavity.

4. The blood slicing device according to claim 3, characterized in that: The plurality of processing components are arranged adjacent to each other along the thickness direction of the glass slide held in the second type glass slide holding cavity, and the plurality of processing components are integrally formed.

5. The blood slicing device according to claim 3, characterized in that: The first type of slide holding chamber further comprises an overflow chamber adjacent to the fixed liquid chamber, and an overflow notch is provided on a side wall shared by the overflow chamber and the fixed liquid chamber. When the glass slide is immersed in the fixative solution in the fixative solution chamber, the overflow gap is used to limit the level of the fixative solution in the fixative solution chamber to be located between the slide pushing area and the printing area. A second flow port for discharging the fixing liquid is provided on the bottom wall of the overflow cavity.

6. The blood slicing device according to claim 5, characterized in that: The overflow chamber is located between the fixing liquid chamber and the second type glass slide holding chamber, and the bottom wall of the second type glass slide holding chamber is provided with a third flow port for discharging the fixing liquid.

7. The blood slicing device according to claim 6, characterized in that: The bottom of the slide box is further provided with a first pipeline and a first main circulation port, and the first main circulation port can be communicated with multiple first circulation ports through the first pipeline.

8. The blood slicing device according to claim 7, characterized in that: The first pipeline extends along the thickness direction of the glass slide held in the second type glass slide holding cavity and passes through the two side walls of the glass slide box. Both ends of the first pipeline are provided with sealing plugs.

9. The blood slicing device according to claim 7, characterized in that: The bottom of the slide box is also provided with a second pipeline, a third pipeline, a second total circulation port and a third total circulation port. The second total circulation port can be connected to multiple second circulation ports through the second pipeline, and the third total circulation port can be connected to multiple third circulation ports through the third pipeline. The first pipeline, the second pipeline and the third pipeline are arranged parallel to each other.

10. The blood slicing device according to claim 1, characterized in that: The top of the second type of slide holding cavity has a second socket for inserting the slide. An air supply channel for supplying air to the second type of slide holding cavity is provided on the cavity wall of the second type of slide holding cavity.

11. The blood slicing device according to claim 10, characterized in that: The air inlet of the air supply channel is arranged near the second socket, and the air outlet of the air supply channel is arranged in the middle of the second type of glass slide holding cavity or near the middle of the second type of glass slide holding cavity to supply air toward the bottom of the second type of glass slide holding cavity.

12. The blood slicing device according to claim 11, characterized in that: The air supply channel forms an angle of 5 to 20 degrees with the glass slide held in the second type glass slide holding cavity.

13. The blood slicing device according to claim 1, characterized in that: First slide placement grooves for holding the slide are respectively provided on two side walls in the width direction of the slide held in the first type slide holding cavity. The first slide placement grooves extend from the top opening of the first type slide holding cavity to the bottom of the first type slide holding cavity along the length direction of the slide held in the first type slide holding cavity. and / or Second glass slide placement grooves for holding the glass slide are respectively provided on the two side walls in the width direction of the glass slide held in the second-type glass slide holding cavity. The second glass slide placement grooves extend from the top opening of the second-type glass slide holding cavity to the bottom of the second-type glass slide holding cavity along the length direction of the glass slide held in the second-type glass slide holding cavity.

14. The blood slicing device according to claim 1, characterized in that: The slide box is in the shape of a rectangular box with an open top.

15. The blood slicing device according to claim 1, characterized in that: The first type of slide holding cavity and the second type of slide holding cavity are both in a rectangular parallelepiped shape matching the shape of the slide.

16. The blood slicing device according to claim 1, characterized in that: The cavity walls constituting the first type of slide holding cavity and the second type of slide holding cavity are integrally formed with the remaining portion of the slide box except the cavity walls.

17. The blood slicing device according to claim 1, characterized in that: The stationary phase is methanol.

18. The blood slicing device according to claim 1, characterized in that: include: A slide transferring component is used to transfer slides between the first type of slide holding chamber and the second type of slide holding chamber.

19. The blood slicing device according to claim 18, characterized in that: Ears are provided on both side walls of the slide box in the thickness direction of the slides held therein. The ears are formed by extending outwards from the corresponding side walls of the slide box, and second positioning protrusions are provided on the ears.

20. The blood slicing device according to claim 19, characterized in that: A mounting cover is provided on the top of the mounting cavity. The mounting cover is provided with an opening for the glass slide to pass through and positioning through holes on both sides of the opening that match the second positioning protrusions. The glass slide box is fixedly connected to the mounting cover.

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