DNA polymerase variants with improved discrimination against genetic variation
By substituting amino acids in the loop region of DNA polymerase, a highly discriminative DNA polymerase variant was developed, solving the specificity and sensitivity issues of PCR technology in detecting very small amounts of variant DNA, and achieving more efficient detection of mutant sequences.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- GENOTECH CO LTD
- Filing Date
- 2021-05-28
- Publication Date
- 2026-07-10
AI Technical Summary
Existing PCR technology struggles to achieve high specificity and sensitivity when detecting very small amounts of variant DNA, especially when mixed with large amounts of wild-type DNA, resulting in a high false positive rate and difficulty in accurately detecting mutant sequences.
By substituting amino acids in the loop region (residues 497-514) of DNA polymerase, particularly by varying the positions of K505, E507, K508, K511, and R512, the ability to distinguish between the 3' end of the primer and the template DNA matching and mismatch was improved, thus developing a DNA polymerase variant with high distinguishability.
It significantly improves the specificity and sensitivity of PCR, enabling more accurate detection of even trace amounts of variant DNA, reducing the false positive rate, and making it suitable for highly sensitive diagnoses such as cancer.
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Figure CN115803434B_ABST