A sterilization method for a bacillus pasteurii strain activity test

Abstract

The application relates to a sterilization method for Bacillus pasteurii strain activity test, which comprises the following steps: S1. medium vessel sterilization; S2. urea solution sterilization; S3. strain starting, uniformly burning the head end of an ampoule bottle, dripping pure water on the burned part to break the head end of the ampoule bottle, then injecting pure water into the ampoule bottle to form a suspension bacterial solution, and continuously burning the outside of the broken part of the ampoule bottle to form a bacterial isolation fire curtain; S4. strain inoculation, placing an inoculation ring in the bacterial isolation fire curtain and burning to be red, stopping burning, and then taking the suspension bacterial solution after the inoculation ring is cooled and transferring the suspension bacterial solution to the culture medium; S5. repeated inoculation sterilization, the bacterial isolation fire curtain needs to be started every time the inoculation ring takes the suspension bacterial solution, and the suspension bacterial solution is taken after the inoculation ring is inoculated and sterilized by the bacterial isolation fire curtain. The application has the effect of effectively avoiding the mixing of other strains in the Bacillus pasteurii strain and affecting the test results of the Bacillus pasteurii strain activity test.

Description

Technical Field

[0001] This application relates to the technical field of bacterial strain sterilization technology, and in particular to a sterilization method for testing the bacterial activity of Bacillus pasteurellis. Background Technology

[0002] Microbial solidification technology utilizes the hydrolysis process of microorganisms in nature to produce large amounts of highly active urease, which hydrolyzes urea. This urea then combines with metal cations in the surrounding environment to form a cementing substance, thereby reinforcing the soil. Microbial solidification technology fills the pores of the soil with the resulting cementing substance, improving the soil's physical and mechanical properties. Domestic and international researchers often use *Sposcoscina pasteuri* for microbial solidification experiments. *Sposcoscina pasteuri* is an aerobic, Gram-positive bacterium that produces large amounts of urease during its metabolism, which can be used to catalyze the hydrolysis of urea.

[0003] Before using Bacillus pasteurization to catalyze urea hydrolysis for microbial solidification, a large number of highly active bacteria must first be cultivated. During the activation process, external contaminants can affect the activity of Bacillus pasteurization. To prevent contamination from other environmental bacteria, the containers used in the cultivation process must be thoroughly sterilized. Special attention must be paid to the sterilization process, especially when using the lyophilized powder in ampoules and before inoculating the culture medium with the inoculation loop. Currently, the operation is mainly carried out manually with frequent operations, and there is still a significant risk of contamination of the bacteria in the ampoules with other bacteria. Summary of the Invention

[0004] To address the issue of Pasteurella multocida strains being susceptible to contamination by other strains during inoculation into culture media, this application provides a sterilization method for testing the bacterial activity of Pasteurella multocida.

[0005] The sterilization method for testing the bacterial activity of Bacillus pasteurellum provided in this application adopts the following technical solution:

[0006] A sterilization method for testing the bacterial activity of Bacillus pasteurellii includes the following steps:

[0007] S1. Sterilize culture media and equipment: Sterilize the culture media, pure water, syringes, PE tubes, sterile pipette tips, and needle filters required for bacterial resuscitation by high temperature and high pressure and ultraviolet sterilization.

[0008] S2. Sterilize the urea solution by filtering and sterilizing the urea solution through a sterilized needle filter on a clean bench.

[0009] S3. To activate the bacterial culture, evenly scorch the top end of the ampoule, then drip pure water onto the scorched area to break the top end of the ampoule. Then, inject pure water into the ampoule to form a suspension of bacterial solution, and continue to scorch the outside of the broken end of the ampoule to form a bacterial barrier.

[0010] S4. Inoculation: Place the inoculation loop in the sterile flame and ignite it until it turns red. Stop igniting and wait for the inoculation loop to cool. Then, dip the inoculation loop into the suspension and transfer it to the culture medium.

[0011] S5. Repeated inoculation and sterilization: After each inoculation loop is dipped into the bacterial suspension, a sterile flame screen must be activated. After the inoculation loop is completed, it should be sterilized by burning with the sterile flame screen before being dipped into the bacterial suspension again.

[0012] Furthermore, when activating the bacterial strain in step S3, the ampoule is placed on an auxiliary sterilization device. The auxiliary sterilization device includes a base and a bracket fixed to the base. A positioning frame for positioning the ampoule is installed on the bracket. The positioning frame is open at the top and is inclined on the bracket. An adsorption element for adsorbing and fixing the ampoule is rotatably provided at the closed end of the positioning frame. A rotating mechanism for rotating the ampoule is provided at the open end of the positioning frame. A burning element with a flame outlet pointing in the direction of the opening of the positioning frame is provided on the base.

[0013] Furthermore, the adsorption component includes a rotating cylinder rotatably mounted on the closed end of the positioning frame and a suction cup fixed to one end of the rotating cylinder near the open end of the positioning frame. An air regulating cylinder is slidably disposed in the rotating cylinder through the middle of the suction cup. The end of the air regulating cylinder near the positioning frame is closed and has an air hole communicating with its hollow part on the side wall of that end. The rotating cylinder is provided with a locking component for locking the air regulating cylinder in the state where the air hole is blocked by the suction cup or the rotating cylinder.

[0014] Furthermore, the locking assembly includes a protrusion fixed to the outer peripheral wall of the air regulating cylinder. The inner wall of the rotating cylinder near the suction cup has a straight groove along its axial direction that slides and adapts to the protrusion. The inner wall of the rotating cylinder also has an inclined groove that slides and adapts to the protrusion and communicates with the straight groove. The inclined groove is circumferentially arranged around the rotating cylinder and its inclined direction points towards the suction cup. When the protrusion slides to the end of the inclined groove away from the straight groove, the air hole is blocked by the suction cup or the rotating cylinder.

[0015] Furthermore, an elastic element is also provided inside the rotating cylinder. One end of the elastic element abuts against the end of the rotating cylinder away from the suction cup, and the other end abuts against the end of the air regulating cylinder away from the suction cup. When the protrusion slides to the end of the inclined groove away from the straight groove, the elastic element is in a compressed state.

[0016] Furthermore, the end of the protrusion near the suction cup is configured as a slope that matches the inclination angle of the groove.

[0017] Furthermore, the rotating mechanism includes an end face gear coaxially fixed to the outside of the rotating drum and a rotating gear rotatably mounted on the positioning frame and meshing with the end face gear, and a handle coaxially fixed to the rotating gear.

[0018] Furthermore, a control rod extending from the end of the air regulating cylinder away from the suction cup is fixedly connected to the end of the end face gear. The teeth of the end face gear are positioned away from the opening end of the positioning frame. A locking rack that meshes with the teeth of the end face gear is fixedly connected to the end of the control rod away from the suction cup. When the air hole on the air regulating cylinder is blocked by the suction cup or the rotating cylinder, the locking rack disengages from the teeth of the end face gear.

[0019] Furthermore, a pull rod is fixedly connected to the end of the air regulating cylinder away from the suction cup.

[0020] In summary, the beneficial technical effects of this application are as follows: after opening the ampoule, by continuing to set an isolation flame at the break point of the ampoule, the isolation flame can separate the break point of the ampoule after the inoculation loop is dipped into the suspension of bacteria. This can effectively prevent other external bacteria from being mixed in with the Bacillus pasteurellis strain and affecting the test results of the Bacillus pasteurellis strain activity test. Attached Figure Description

[0021] Figure 1 This is a flowchart of a sterilization method according to an embodiment of this application.

[0022] Figure 2 This is a schematic diagram of the overall structure of the auxiliary sterilization device according to an embodiment of this application.

[0023] Figure 3 This is a longitudinal sectional view of the auxiliary sterilization device according to an embodiment of this application.

[0024] Figure 4 yes Figure 3 A magnified view of part A in the diagram.

[0025] Figure 5 This embodiment of the application is mainly used to illustrate the structural diagram of the inclined groove and inclined surface of the auxiliary sterilization equipment.

[0026] Reference numerals: 1. Base; 2. Bracket; 3. Positioning frame; 4. Burning component; 5. Rotating drum; 6. Suction cup; 7. Air regulating cylinder; 8. Air hole; 9. Protrusion; 10. Straight groove; 11. Inclined groove; 12. Elastic component; 13. Inclined surface; 14. End face gear; 15. Rotating gear; 16. Handle; 17. Control lever; 18. Locking rack; 19. Pull rod; 20. Sealing ring; 21. Limiting ring. Detailed Implementation

[0027] The technical solutions of this application will now be clearly and completely described with reference to the accompanying drawings. Obviously, the described embodiments are only some, not all, of the embodiments of this application. Based on the embodiments of this application, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this application.

[0028] This application discloses a sterilization method for testing the bacterial activity of Bacillus pasteurellii. (Refer to...) Figure 1 The sterilization method for the bacterial viability test of Bacillus pasteurellis includes the following steps:

[0029] S1. Sterilization of culture medium and equipment: The instruments and reagents required for bacterial resuscitation, such as culture medium, pure water, syringes, PE tubes, sterile pipette tips, needle filters, etc., are sterilized by high temperature and high pressure as well as ultraviolet sterilization.

[0030] S2. Sterilize the urea solution by filtering and sterilizing the urea solution through a sterilized needle filter on a clean bench.

[0031] S3. To activate the bacterial culture, evenly scorch the top end of the ampoule, then drip pure water onto the scorched area to break the top end of the ampoule. Then, inject pure water into the ampoule to form a suspension of bacterial solution, and continue to scorch the outside of the broken end of the ampoule to form a bacterial barrier.

[0032] S4. Inoculation: Place the inoculation loop in the sterile flame and ignite it until it turns red. Stop igniting and wait for the inoculation loop to cool. Then, dip the inoculation loop into the suspension and transfer it to the culture medium.

[0033] S5. Repeated inoculation and sterilization: After each inoculation loop is dipped into the bacterial suspension, a sterile flame screen must be activated. After the inoculation loop is completed, it should be sterilized by burning with the sterile flame screen before being dipped into the bacterial suspension again.

[0034] In step S3, when activating the bacterial culture, the ampoule is placed on the auxiliary sterilization equipment, as shown in Figure 2. Figure 3The auxiliary sterilization equipment includes a base 1 and a bracket 2 fixed to the base 1. A positioning frame 3 for positioning ampoules is mounted on the bracket 2. The positioning frame 3 is a cylindrical shape with an open top and is installed at an angle on the bracket 2. Specifically, a collar is fitted onto the bracket 2, and a bolt with its head end abutting against the bracket 2 is threaded through the collar. A positioning ring is fixed to the outer wall of the collar's arc surface. A positioning rod is fixed to the positioning frame 3, passing through the positioning ring, and a bolt with its head end abutting against the positioning rod is threaded through the positioning ring. The closed end of the positioning frame 3 is rotatably equipped with an adsorption element for adsorbing and fixing the bottom of the ampoule. The open end of the positioning frame 3 is equipped with a rotating mechanism for rotating the ampoule. The base 1 is equipped with a burning element 4 with its flame outlet pointing towards the opening of the positioning frame 3. The burning element 4 can be an alcohol lamp, an alcohol torch, or a lighter, etc. In this embodiment, an alcohol lamp is selected as the burning element 4.

[0035] When using the bacterial culture, the ampoule is first inserted into the positioning frame 3 and fixed by the adsorption element. Then, the ignition element 4 is turned on. The position of the positioning frame 3 is adjusted by loosening the bolt and adjusting the rotation angle of the support 2 on the positioning ring and the vertical position of the adjusting collar on the support 2, so that the head of the ampoule on the positioning frame 3 is aligned with the ignition element 4 and tilted upward. Then, the ampoule is rotated by the rotating mechanism so that the ignition element 4 evenly ignites the head of the ampoule. After ignition for a certain period of time, the ignition element 4 is removed or extinguished. Pure water is dripped onto the ignition part of the ampoule. The ignition part of the ampoule is heated and then pre-cooled and ruptured, realizing convenient opening of the ampoule without the experimenter holding the ampoule. It can effectively isolate contamination and reduce the probability of the bacterial culture in the ampoule being contaminated by other bacterial cultures.

[0036] Once the ampoule is opened, by placing the flame of the burning element 4 at the break point of the ampoule and continuing to set up a sterile flame barrier, the sterile flame barrier can separate the break point of the ampoule. This ensures that after the inoculation loop is dipped into the suspension of bacteria, the sterile flame barrier can effectively prevent other external bacteria from being mixed with the Bacillus pasteurellis strain and affecting the test results of the Bacillus pasteurellis strain activity test. This ensures that the Bacillus pasteurellis strain is always free from interference from other bacteria during the process of taking the Bacillus pasteurellis strain.

[0037] More specifically, refer to Figure 3 and Figure 4The adsorption component includes a rotating cylinder 5 rotatably mounted on the closed end of the positioning frame 3 and a suction cup 6 fixed to one end of the rotating cylinder 5 near the open end of the positioning frame 3. Both the rotating cylinder 5 and the suction cup 6 are coaxially arranged with the cylindrical positioning frame 3. An air regulating cylinder 7 is provided in the middle of the suction cup 6 and is slidably disposed in the rotating cylinder 5. The end of the air regulating cylinder 7 near the positioning frame 3 is closed and has an air hole 8 on its side wall that communicates with its hollow part. Specifically, a sealing ring 20 that is compatible with the rotating cylinder 5 is embedded in the side of the air hole 8 near the suction cup 6. The rotating cylinder 5 is provided with a locking component for locking the air regulating cylinder 7 in the state where the air hole 8 is blocked by the suction cup 6 or the rotating cylinder 5. In this embodiment, the locking component is used to lock the air regulating cylinder 7 in the state where the air hole 8 is blocked by the rotating cylinder 5.

[0038] Among them, reference Figure 4 and Figure 5 The locking assembly includes a protrusion 9 fixed to the outer peripheral wall of the air regulating cylinder 7. A straight groove 10, slidably fitted to the protrusion 9, is formed on the inner wall of the rotating cylinder 5 near the suction cup 6. An inclined groove 11, slidably fitted to the protrusion 9 and communicating with the straight groove 10, is also formed on the inner wall of the rotating cylinder 5. The inclined groove 11 is circumferentially arranged around the rotating cylinder 5, with its inclination pointing towards the suction cup 6. The angle between the inclined groove 11 and the straight groove 10 is less than 90 degrees and greater than 80 degrees. When the protrusion 9 slides to the end of the inclined groove 11 away from the straight groove 10, the air hole 8 is blocked by the suction cup 6 or the rotating cylinder 5. Furthermore, a pull rod 19, extending out of the rotating cylinder 5 and the positioning frame 3, is fixed to the end of the air regulating cylinder 7 away from the suction cup 6.

[0039] With this setup, when the ampoule is inserted into the positioning frame 3, the bottom of the ampoule contacts the suction cup 6. As the ampoule is gradually inserted, the air between its bottom and the suction cup 6 is expelled through the air hole 8 on the air regulating cylinder 7 and the air regulating cylinder 7 itself, allowing the suction cup 6 to stably adsorb the ampoule. During this process, the air regulating cylinder 7 is also pushed by the bottom of the ampoule and slides in the rotating cylinder 5. At the same time, the protrusion 9 on the air regulating cylinder 7 slides in the straight groove 10 on the inner wall of the rotating cylinder 5. When the protrusion 9 slides to the end of the straight groove 10 near the inclined groove 11, the air hole 8 on the air regulating cylinder 7 is embedded in the rotating cylinder 5. The sealing ring 20 seals and separates the air hole 8 from the cavity of the suction cup 6, making the cavity between the suction cup 6 and the bottom wall of the ampoule as close to a vacuum as possible, which can ensure the strong adsorption effect of the suction cup 6 on the ampoule.

[0040] Then, by rotating the air regulating cylinder 7 through the pull rod 19, the protrusion 9 slides along the inclined groove 11 and moves to the end of the inclined groove 11 away from the straight groove 10. At this time, the protrusion 9 is locked in the inclined groove 11 and cannot move towards the opening end of the positioning frame 3. That is, the air regulating cylinder 7 is locked and cannot move towards the ampoule. At this time, the suction cup 6 maintains a firm adsorption state on the ampoule so that the rotating mechanism can smoothly rotate the ampoule to perform the opening operation.

[0041] When it is necessary to remove the ampoule from the positioning frame 3, simply rotate the air regulating cylinder 7 in the opposite direction so that the protrusion 9 moves to the junction of the inclined groove 11 and the straight groove 10. Then push the air regulating cylinder 7 towards the opening end of the positioning frame 3 until the air hole 8 on the air regulating cylinder 7 connects with the cavity between the suction cup 6 and the bottom wall of the ampoule. External air enters the cavity between the suction cup 6 and the bottom wall of the ampoule, which can release the suction effect of the suction cup 6 on the bottom wall of the ampoule, thereby causing the suction cup 6 to detach from the ampoule, and the ampoule can be easily removed from the positioning frame 3.

[0042] To facilitate the insertion of the ampoule into the air pump 7, the ampoule is automatically and securely attached to the suction cup 6, as per [reference needed]. Figure 3 and Figure 4 The rotating cylinder 5 is also equipped with an elastic element 12. One end of the elastic element 12 abuts against the end of the rotating cylinder 5 away from the suction cup 6, and the other end abuts against the end of the air regulating cylinder 7 away from the suction cup 6. Specifically, the elastic element 12 is a spring. A limiting ring 21 is fixedly connected to the periphery of the air regulating cylinder 7 and slides to match the inner wall of the opening end of the rotating cylinder 5 away from the positioning frame 3. The elastic element 12 is sleeved on the periphery of the air regulating cylinder 7, with one end abutting against the limiting ring 21 and the other end abutting against the bottom wall of the rotating cylinder 5. When the protrusion 9 slides to the end of the inclined groove 11 away from the straight groove 10, the elastic element 12 is in a compressed state. Moreover, the end of the protrusion 9 near the suction cup 6 is set as an inclined surface 13 that matches the inclination angle of the inclined groove 11.

[0043] In this way, when the ampoule bottle pushes against the gas regulating cylinder 7, the limiting ring 21 pushes the elastic element 12 to compress until the protrusion 9 slides to the connection between the straight groove 10 and the inclined groove 11. The compressed elastic element 12 has the tendency to push the protrusion 9 towards the suction cup 6. At this time, since the inclined surface 13 of the protrusion 9 is in contact with the inclined wall of the inclined groove 11, under this guiding action, the gas regulating cylinder 7 drives the protrusion 9 to rotate, so that the protrusion 9 slides in the inclined groove 11 to the end of the inclined groove 11 away from the straight groove 10. At this time, the elastic element 12 is still in a compressed state, which can press the protrusion 9 against the inclined groove 11, that is, lock the gas regulating cylinder 7 in the state where its air hole 8 is blocked by the rotating cylinder 5, which can ensure the firm adsorption effect of the suction cup 6 on the ampoule bottle.

[0044] Similarly, when the ampoule needs to be removed, press the ampoule again to push the gas regulating cylinder 7 to rotate in the opposite direction in the rotating cylinder 5 until the protrusion 9 slides along the inclined groove 11 to the connection between the sliding groove and the straight groove 10. Release the ampoule, and the deformation force of the elastic element 12 pushes the protrusion 9 to slide along the straight groove 10. As a result, the gas regulating cylinder 7 slides in the rotating cylinder 5 towards the suction cup 6, so that the air hole 8 on the gas regulating cylinder 7 connects with the cavity between the suction cup 6 and the bottom wall of the ampoule. This can release the adsorption effect of the suction cup 6 on the bottom wall of the ampoule, thereby realizing the semi-automatic adsorption and contact adsorption effect of the adsorption element on the ampoule.

[0045] To facilitate easy rotation of the ampoule, refer to... Figure 3 The rotating mechanism includes an end face gear 14 coaxially fixed to the outside of the rotating cylinder 5 and a rotating gear 15 rotatably mounted on the positioning frame 3 and meshing with the end face gear 14. The teeth of the end face gear 14 are arranged facing away from the opening end of the positioning frame 3. A handle 16 is coaxially fixed to the rotating gear 15. A control rod 17 extending to protrude from the end face gear 14 is fixed to the end of the air regulating cylinder 7 away from the suction cup 6. A locking rack 18 that meshes with the teeth of the end face gear 14 is fixed to the end of the control rod 17 away from the suction cup 6. When the air hole 8 on the air regulating cylinder 7 is blocked by the suction cup 6 or the rotating cylinder 5, the locking rack 18 disengages from the teeth of the end face gear 14.

[0046] After this setup, once the ampoule is inserted into the positioning frame 3 and attracted by the suction cup 6, the air regulating cylinder 7 drives the control lever 17, causing the locking rack 18 on the control lever 17 to move away from the end face gear 14. At this time, the locking rack 18 disengages from the end face gear 14. Turning the handle 16 can drive the rotating gear 15 to rotate, which in turn drives the end face gear 14 to rotate. Meanwhile, the ampoule is firmly attracted to the suction cup 6. When the end face gear 14 rotates, it drives the rotating cylinder 5, the suction cup 6, and the ampoule to rotate synchronously, thereby achieving a uniform burning effect on the end of the ampoule by the burning element 4, which is more conducive to the convenient and efficient use of the ampoule.

[0047] The principle of the sterilization method for the bacterial activity test of Bacillus pasteurellus in this application embodiment is as follows: After opening the ampoule, by continuing to set the sterile flame at the cut end of the ampoule, the sterile flame can separate the cut end of the ampoule after the inoculation loop is dipped into the suspension of bacteria. This can effectively prevent other external bacteria from being mixed in with the Bacillus pasteurellus strain and affecting the test results of the bacterial activity test of Bacillus pasteurellus.

[0048] The above are all preferred embodiments of this application, and are not intended to limit the scope of protection of this application. Therefore, all equivalent changes made in accordance with the structure, shape and principle of this application should be covered within the scope of protection of this application.

Claims

1. A sterilization method for testing the bacterial activity of Bacillus pasteurellii, characterized in that, Includes the following steps: S1. Sterilize culture media and equipment: Sterilize the culture media, pure water, syringes, PE tubes, sterile pipette tips, needle filters and other items required for bacterial resuscitation by high temperature and high pressure and ultraviolet sterilization. S2. Sterilize the urea solution by filtering and sterilizing the urea solution through a sterilized needle filter on a clean bench. S3. To activate the bacterial culture, evenly scorch the top end of the ampoule, then drip pure water onto the scorched area to break the top end of the ampoule. Then, inject pure water into the ampoule to form a suspension of bacterial solution, and continue to scorch the outside of the broken end of the ampoule to form a bacterial barrier. S4. Inoculation: Place the inoculation loop in the sterile flame and ignite it until it turns red. Stop igniting and wait for the inoculation loop to cool. Then, dip the inoculation loop into the suspension and transfer it to the culture medium. S5. Repeated inoculation and sterilization: After each inoculation loop is dipped into the bacterial suspension, a sterile flame screen must be activated. After inoculation, the inoculation loop should be sterilized by flame screen before being dipped into the bacterial suspension again. When activating the bacterial strain in step S3, the ampoule is placed on an auxiliary sterilization device. The auxiliary sterilization device includes a base (1) and a bracket (2) fixed to the base (1). A positioning frame (3) for positioning the ampoule is installed on the bracket (2). The positioning frame (3) is open at the top and is inclined on the bracket (2). An adsorption element for adsorbing and fixing the ampoule is rotatably provided at the closed end of the positioning frame (3). A rotating mechanism for rotating the ampoule is provided at the open end of the positioning frame (3). A burning element (4) with a flame outlet pointing in the direction of the opening of the positioning frame (3) is provided on the base (1). The adsorption component includes a rotating cylinder (5) rotatably mounted on the closed end of the positioning frame (3) and a suction cup (6) fixed to one end of the rotating cylinder (5) near the open end of the positioning frame (3). An air regulating cylinder (7) is slidably disposed in the rotating cylinder (5) through the middle of the suction cup (6). The end of the air regulating cylinder (7) near the positioning frame (3) is closed and an air hole (8) communicating with its hollow part is opened on the side wall of that end. A locking component is provided in the rotating cylinder (5) for locking the air regulating cylinder (7) in the state where the air hole (8) is blocked by the suction cup (6) or the rotating cylinder (5). The locking assembly includes a protrusion (9) fixed to the outer peripheral wall of the air regulating cylinder (7). The inner wall of the rotating cylinder (5) near the suction cup (6) has a straight groove (10) that slides along its axial direction and is adapted to the protrusion (9). The inner wall of the rotating cylinder (5) also has an inclined groove (11) that slides to the protrusion (9) and communicates with the straight groove (10). The inclined groove (11) is arranged around the rotating cylinder (5) and its inclined direction points to the suction cup (6). When the protrusion (9) slides to the end of the inclined groove (11) away from the straight groove (10), the air hole (8) is blocked by the suction cup (6) or the rotating cylinder (5). The rotating mechanism includes an end face gear (14) coaxially fixed to the outside of the rotating drum (5) and a rotating gear (15) rotatably mounted on the positioning frame (3) and meshing with the end face gear (14). A handle (16) is coaxially fixed to the rotating gear (15).

2. The sterilization method for testing the bacterial activity of Bacillus pasteurellium according to claim 1, characterized in that, The rotating drum (5) is also provided with an elastic element (12). One end of the elastic element (12) abuts against the end of the rotating drum (5) away from the suction cup (6), and the other end abuts against the end of the air regulating cylinder (7) away from the suction cup (6). When the protrusion (9) slides to the end of the inclined groove (11) away from the straight groove (10), the elastic element (12) is in a compressed state.

3. The sterilization method for testing the bacterial activity of Bacillus pasteurellium according to claim 2, characterized in that, The end of the protrusion (9) near the suction cup (6) is configured as a slope (13) that matches the inclination angle of the groove (11).

4. The sterilization method for testing the bacterial activity of Bacillus pasteurellium according to claim 3, characterized in that, The end of the air regulating cylinder (7) away from the suction cup (6) is fixedly connected to a control rod (17) extending to protrude from the end face gear (14). The teeth of the end face gear (14) are set away from the opening end of the positioning frame (3). The end of the control rod (17) away from the suction cup (6) is fixedly connected to a locking rack (18) that meshes with the teeth of the end face gear (14). When the air hole (8) on the air regulating cylinder (7) is blocked by the suction cup (6) or the rotating cylinder (5), the locking rack (18) disengages from the teeth of the end face gear (14).

5. The sterilization method for testing the bacterial activity of Bacillus pasteurellium according to claim 4, characterized in that, A pull rod (19) is fixed to the end of the air regulating cylinder (7) away from the suction cup (6).

Images