An experimental rat abdominal aorta fixation blood collection device

Abstract

This invention discloses an experimental rat abdominal aorta fixation and blood collection device, belonging to the field of animal fixation and blood collection technology. It includes an experimental body with an operating table mounted on its upper end. The operating table has a Velcro structure in the middle and an absorbent pad is attached. Four side plates are vertically fixed at the four corners of the experimental body, and supplementary lights are mounted on the opposite sides of each side plate. Four movable slots are formed near the four corners of the operating table, and four magnetic restraint devices are movably connected within these slots. A controller is fixed to the front end of the experimental body and electrically connected to an external power source. This invention provides that the four magnetic restraint devices can be adjusted according to the length of the rat's limbs, and the controller controls four electromagnets to attract two iron blocks on four elastic restraint straps, completing rapid restraint and fixation. The operation is relatively simple and convenient, improving the efficiency of blood collection.

Description

Technical Field

[0001] This invention relates to the field of animal fixation and blood collection technology, and in particular to an experimental rat abdominal aorta fixation and blood collection device. Background Technology

[0002] In medical research, animal experiments are generally used to study the life phenomena of animals and then apply the findings to humans to explore the mysteries of human life, control human diseases and aging, and extend human lifespan. The most commonly used animal subjects in animal experiments are rats and hamsters. When testing the levels of routine and biochemical indicators in the body's blood, rat blood needs to be collected. Currently, blood collection methods include abdominal aortic blood collection, carotid artery and vein blood collection, femoral artery blood collection, tail vein blood collection, and cardiac blood collection. The abdominal aortic blood collection method is as follows: First, the animal is anesthetized and fixed supine on the operating table. The abdominal cavity is incised along the midline of the abdomen. Using forceps, the abdominal organs are turned to the left or right, and the fat in front of the spine is gently separated to reveal them. The fascia tissue next to the abdominal aorta is dissected with small forceps, and a section of the aorta is held separately with two vascular clamps. The arterial wall is gently lifted with small forceps, and then the abdominal aorta is punctured using a blood collection tube. After blood return is observed in the abdominal aorta, the other end is connected to a syringe for blood extraction.

[0003] Currently, the operating table used for blood collection from the abdominal aorta of rats is relatively simple and has limited functionality. After anesthetizing the rats, researchers need to spend a lot of time binding the rats' limbs (rat limbs are relatively small and binding is troublesome), and blood collection requires manual extraction, which is relatively inefficient.

[0004] Therefore, it is necessary to design an experimental rat abdominal aorta fixation and blood collection device to solve the above problems. Summary of the Invention

[0005] This invention provides an experimental rat abdominal aorta fixation and blood collection device, which solves the above-mentioned technical problems.

[0006] To solve the above-mentioned technical problems, the present invention provides an experimental rat abdominal aortic fixation and blood collection device, comprising an experimental body, an operating table installed on the upper end of the experimental body, and a Velcro structure with an absorbent pad attached to the middle of the operating table. Four side plates are vertically fixed at the four corners of the experimental body, and supplementary lights are installed on the opposite sides of the four side plates. Four movable slots are opened near the four corners of the operating table, and four magnetic restraint devices are movably connected in the four movable slots. A controller is fixed at the front end of the experimental body and electrically connected to an external power source. A negative pressure extraction mechanism is fixed at the side end of the experimental body. A blood collection needle is connected through the top of the negative pressure extraction mechanism, and a disposable blood collection bottle is embedded in the middle of the negative pressure extraction mechanism.

[0007] Furthermore, the magnetic restraint device includes a movable block and a movable box. The movable block is movably installed in the movable slot, and two through slots are opened on both sides of the movable block, through which the two ends of the elastic restraint strap are inserted. The movable box is fixed to the bottom of the operating table, and two iron blocks are set inside the movable box and fixed separately at the two ends of the elastic restraint strap. An electromagnet is fixedly installed at the bottom of the movable box.

[0008] Furthermore, the internal length of the movable box is equal to the length of the movable slot.

[0009] Furthermore, the movable block is installed in the movable slot with an I-shaped cross-section.

[0010] Furthermore, the negative pressure extraction mechanism includes a vacuum pump and a mounting base. The mounting base is fixed to the side of the experimental body, and an inlay groove is provided on one side of the mounting base. A connecting sleeve is fixed at the top of the inlay groove, and one end of the negative pressure tube and the delivery tube are respectively connected through the inside of the connecting sleeve. The delivery tube passes through the top of the inlay groove and is provided with a delivery head that is connected to the blood collection needle. The other end of the negative pressure tube is connected to the negative pressure interface of the vacuum pump, and the vacuum pump is fixedly installed inside the experimental body. An embedding groove is provided at the bottom of the inlay groove, and a spring is fixedly connected to the bottom of the embedding groove. A stop plate is fixedly connected to the top of the spring.

[0011] Furthermore, the disposable blood collection bottle is made of transparent PET material, and a scale is provided on the outer surface of the disposable blood collection bottle. A connecting plug is connected through the top of the disposable blood collection bottle, and a rubber gasket is fitted on the outside of the connecting plug.

[0012] Furthermore, the outer diameter of the connector plug is equal to the inner diameter of the connector sleeve, and the outer diameter of the disposable blood collection bottle is equal to the inner diameter of the embedding groove.

[0013] Furthermore, the disposable blood collection bottle is inserted into the embedding groove, and the rubber gasket is embedded in the connecting sleeve, and the connecting sleeve and the rubber gasket are in contact and sealed.

[0014] Furthermore, the controller is electrically connected to four supplementary lights, four electromagnets, and a vacuum pump.

[0015] Compared with related technologies, the experimental rat abdominal aorta fixation and blood collection device provided by the present invention has the following beneficial effects:

[0016] This invention provides a method that uses four magnetic restraint devices to adjust the position of the rat according to the length of its limbs, and controls four electromagnets through a controller to attract two iron blocks on four elastic restraint bands, thereby completing rapid restraint and fixation. This method is relatively simple and convenient to operate, and improves the efficiency of blood collection.

[0017] The present invention provides that, during dissection, the operating table is tilted to facilitate dissection towards the experimenter, and supplemental lighting is provided to ensure sufficient illumination. Furthermore, blood that seeps into the operating table during dissection is absorbed by an absorbent pad to prevent the operating table from becoming contaminated.

[0018] This invention provides a method for inserting a blood collection needle connected to a negative pressure extraction mechanism into the aorta of a rat. A vacuum pump is controlled by a controller to operate, and the vacuum pump begins to extract air, creating negative pressure in a disposable blood collection bottle through a negative pressure tube. This allows the blood collection needle to open and draw blood from the rat's aorta. The blood is then stored in the disposable blood collection bottle. The experimenter determines the amount of blood drawn using a scale, and once the desired amount is reached, the controller stops the vacuum pump. The operation is relatively convenient.

[0019] This invention provides a disposable blood collection bottle that is tilted and inserted into an embedding groove, where it contacts and is pressed against a backing plate. The spring is compressed, and the disposable blood collection bottle is fully inserted into the embedding groove and aligns with the connecting sleeve. As the disposable blood collection bottle is released, the spring causes the backing plate to rebound, and the disposable blood collection bottle rises, inserting the connecting plug into the connecting sleeve. The plug is then pressed against a rubber gasket to maintain a seal. Pressing the disposable blood collection bottle down further separates the connecting plug from the connecting sleeve, allowing the disposable blood collection bottle to be removed for use. This method is relatively convenient and reusable. Attached Figure Description

[0020] Figure 1 This is a schematic diagram of the overall invention;

[0021] Figure 2 This is a cross-sectional schematic diagram of the magnetic confinement device of the present invention;

[0022] Figure 3 This is a schematic diagram of the magnetic confinement device of the present invention;

[0023] Figure 4 This is a schematic diagram of the negative pressure extraction mechanism of the present invention;

[0024] Figure 5 This is a schematic cross-sectional view of the negative pressure extraction mechanism of the present invention;

[0025] Figure 6 This is a schematic diagram of the disposable blood collection bottle of the present invention.

[0026] Numbered in the diagram: 1. Main experimental body; 11. Moving trough; 12. Operating table; 2. Side panel; 21. Supplemental light; 3. Water-absorbing pad; 4. Magnetic restraint device; 41. Elastic restraint belt; 411. Iron block; 42. Moving block; 421. Through groove; 43. Moving box; 44. Electromagnet; 6. Negative pressure extraction mechanism; 61. Vacuum pump; 62. Mounting base; 63. Embedding groove; 631. Inserting groove; 632. Support plate; 633. Spring; 64. Conveying head; 641. Conveying pipe; 65. Connecting sleeve; 66. Negative pressure pipe; 7. Disposable blood collection bottle; 71. Scale; 72. Connecting plug; 73. Rubber ring gasket; 8. Blood collection needle; 9. Controller. Detailed Implementation

[0027] Example 1, by Figure 1-6 A rat abdominal aortic fixation and blood collection device is provided, comprising an experimental body 1, an operating table 12 mounted on the upper end of the experimental body 1, and a Velcro structure with an absorbent pad 3 attached to the middle of the operating table 12. Four side plates 2 are vertically fixed at the four corners of the experimental body 1, and supplementary lights 21 are mounted on the opposite sides of the four side plates 2. Four movable slots 11 are opened near the four corners of the operating table 12, and four magnetic restraint devices 4 are movably connected in the four movable slots 11. A controller 9 is fixed at the front end of the experimental body 1 and electrically connected to an external power source. A negative pressure extraction mechanism 6 is fixed at the side end of the experimental body 1. A blood collection needle 8 is connected through the top of the negative pressure extraction mechanism 6, and a disposable blood collection bottle 7 is embedded in the middle of the negative pressure extraction mechanism 6.

[0028] During blood collection, the controller 9 activates four supplementary lights 21 to illuminate the operating table 12. The rat is then anesthetized and placed on the operating table, lying supine on the table 12 with the absorbent pad 3 in contact with it. The abdominal cavity is then incised along the midline using surgical scissors, and the abdominal organs are rotated to the left or right using hemostatic forceps. Under the penetrating light of the LED lights, the direction and location of the blood vessels can be clearly seen. The location is selected 1-3 mm from the bifurcation of the abdominal aorta towards the heart. To obtain the optimal puncture point, a blood collection needle 8 connected to a negative pressure extraction mechanism 6 is used. The needle tip is angled upwards at a 5°–15° angle to the abdominal aorta. The needle is inserted quickly, accurately, and steadily from the distal end to the central end to collect blood. The operating table 12 is tilted, with an angle of 10°–40° relative to the horizontal plane, to facilitate dissection and blood collection towards the experimenter. Sufficient light is provided by supplemental lighting 21. Blood that seeps out during dissection falls onto the operating table 12 and is absorbed by the absorbent pad 3 to prevent the operating table 12 from becoming contaminated.

[0029] The controller 9 is electrically connected to four supplementary lights 21, four electromagnets 44 and a vacuum pump 61.

[0030] In this embodiment, the magnetic restraint device 4 includes a movable block 42 and a movable box 43. The movable block 42 is movably installed in the movable slot 11, and two through slots 421 are opened through both sides of the movable block 42, into which the two ends of the elastic restraint strap 41 are inserted. The movable box 43 is fixed to the bottom of the operating table 12, and two iron blocks 411 are provided inside the movable box 43 and are fixed separately at the two ends of the elastic restraint strap 41. An electromagnet 44 is fixedly installed at the bottom of the movable box 43.

[0031] Specifically, the positions of the four magnetic restraint devices 4 are adjusted according to the length of the rat's limbs. First, the elastic restraint band 41 is stretched to its maximum, and the two iron blocks 411 are in contact with the moving blocks and limited. Then, the moving blocks 42 are pushed on the moving slots 11 to move the elastic restraint band 41 onto one of the rat's limbs. The other three magnetic restraint devices 4 are in the same way. Then, the controller 9 controls the four electromagnets 44 to work. When the four electromagnets 44 are energized, they generate magnetic force to attract and fix the two iron blocks 411 connected to the four elastic restraint bands 41. The four elastic restraint bands 41 contract and tighten, fixing the rat's limbs to the four moving blocks 42 for fixed limitation.

[0032] The internal length of the moving box 43 is equal to the length of the moving groove 11; the moving block 42 is installed in the moving groove 11 with an I-shaped cross section; the moving block 42 is pushed on the moving groove 11 to move it so that the elastic restraint band 41 is put into one of the rat's limbs. The moving block 42 has an I-shaped cross section and is not easy to detach from the moving groove 11.

[0033] In Example 2, based on Example 1, the negative pressure extraction mechanism 6 includes a vacuum pump 61 and a mounting base 62. The mounting base 62 is fixed to the side of the experimental body 1, and an inlay groove 63 is provided on one side of the mounting base 62. A connecting sleeve 65 is fixed at the top of the inlay groove 63, and one end of a negative pressure tube 66 and a delivery tube 641 are respectively connected through the inside of the connecting sleeve 65. The delivery tube 641 passes through the top of the inlay groove 63 and is provided with a delivery head 64 that is connected through to the blood collection needle 8. The other end of the negative pressure tube 66 is connected through to the negative pressure interface of the vacuum pump 61, and the vacuum pump 61 is fixedly installed inside the experimental body 1. An embedding groove 631 is provided at the bottom of the inlay groove 63, and a spring 633 is fixedly connected at the bottom of the embedding groove 631. A stop plate 632 is fixedly connected to the top of the spring 633.

[0034] Specifically, the blood collection needle 8, connected to the negative pressure extraction mechanism 6, is inserted into the rat's aorta. The vacuum pump 61 is controlled by the controller 9 to operate. The vacuum pump 61 starts to extract air, creating negative pressure in the disposable blood collection bottle 7 through the negative pressure tube 66. This allows the blood collection needle 8 to open and draw blood from the rat's aorta. The blood is stored in the disposable blood collection bottle 7. The experimenter uses the scale 71 to determine the amount of blood drawn. Once the target value is reached, the controller 9 stops the vacuum pump 61. The operation is relatively convenient.

[0035] The disposable blood collection bottle 7 is made of transparent PET material, and a scale 71 is provided on the outer surface of the disposable blood collection bottle 7. A connecting plug 72 is connected through the top of the disposable blood collection bottle 7, and a rubber ring gasket 73 is sleeved on the outside of the connecting plug 72.

[0036] The outer diameter of the connector 72 is equal to the inner diameter of the connector sleeve 65, and the outer diameter of the disposable blood collection bottle 7 is equal to the inner diameter of the embedding groove 631. The disposable blood collection bottle is inserted into the embedding groove 631, and the rubber gasket 73 is embedded in the connector sleeve 65, and the connector sleeve 65 and the rubber gasket 73 are in contact and sealed.

[0037] Specifically, before use, the disposable blood collection bottle 7 is tilted and inserted into the embedding groove 631 to contact the abutment plate 632. As the abutment plate 632 is pressed down, it causes the spring 633 to compress, and the disposable blood collection bottle 7 is fully inserted into the embedding groove 63 and aligns with the connecting sleeve 65. As the disposable blood collection bottle 7 is released, the spring 633 causes the abutment plate 632 to rebound, and the disposable blood collection bottle 7 rises to insert the connecting plug 72 into the connecting sleeve 65. By contacting and squeezing with the rubber gasket 73, it is kept sealed. After completion, the disposable blood collection bottle 7 is pressed down to separate the connecting plug 72 from the connecting sleeve 65, and the disposable blood collection bottle 7 can be taken out for use. It is relatively convenient and can be reused.

[0038] Working principle:

[0039] Before use, the disposable blood collection bottle 7 is tilted and inserted into the embedding groove 631 to contact the backing plate 632. As the backing plate 632 is pressed down, it causes the spring 633 to compress, and the disposable blood collection bottle 7 is fully inserted into the embedding groove 63 and aligns with the connecting sleeve 65. As the disposable blood collection bottle 7 is released, the spring 633 causes the backing plate 632 to rebound, and the disposable blood collection bottle 7 rises to insert the connecting plug 72 into the connecting sleeve 65. It is also squeezed by contact with the rubber gasket 73 to keep it sealed.

[0040] During blood collection, the controller 9 controls the four supplementary lights 21 to illuminate the operating table 12. Then, the rat is placed on the operating table for anesthesia. The anesthetized rat is laid supine on the operating table 12 and in contact with the absorbent pad 3.

[0041] Then, the positions of the four magnetic restraint devices 4 are adjusted according to the length of the rat's limbs. First, the elastic restraint band 41 is pulled open to the maximum, and the two iron blocks 411 are in contact with the moving block 11 for limitation. Then, the moving block 42 is pushed on the moving slot 11 to move so that the elastic restraint band 41 is put into one of the rat's limbs. The other three magnetic restraint devices 4 are in the same way. Then, the controller 9 controls the four electromagnets 44 to work. The four electromagnets 44 generate magnetic force when energized, which attracts and fixes the two iron blocks 411 connected to the four elastic restraint bands 41. The four elastic restraint bands 41 contract and tighten, fixing the rat's limbs to the four moving blocks 42 for fixed limitation.

[0042] The four magnetic restraint devices can be adjusted according to the length of the rat's limbs, and the controller 9 controls the four electromagnets 44 to attract the two iron blocks 411 on the four elastic restraint bands 41, thus completing the rapid restraint and fixation. The operation is relatively simple and convenient, and the efficiency of blood collection is improved.

[0043] Using surgical scissors, cut open the abdominal cavity along the midline of the abdomen, and use hemostatic forceps to flip the abdominal organs to the left or right. Under the illumination of a high-penetration LED light, the direction and location of the blood vessels can be clearly seen. Select the best puncture point as 1-3 mm from the bifurcation of the abdominal aorta towards the heart. Use the blood collection needle 8 connected to the negative pressure extraction mechanism 6, with the bevel of the needle tip facing upward, at an angle of 5° to 15° with the abdominal aorta, and quickly, accurately, and steadily insert the needle from the distal end towards the heart to collect blood.

[0044] In summary, the operating table 12 is tilted, with an angle of inclination of 10° to 40° relative to the horizontal plane, which facilitates dissection and blood collection towards the experimental personnel. Sufficient light is provided by the supplementary light lamp 21, and the blood that seeps out during dissection falls onto the operating table 12 and is absorbed by the absorbent pad 3, preventing the operating table 12 from becoming contaminated.

[0045] The blood collection needle 8 is inserted into the rat's aorta. The vacuum pump 61 is controlled by the controller 9 to start working. The vacuum pump 61 starts to draw air (this vacuum pump 61 is a miniature vacuum pump 61 with low pressure. Blood collection is prevented from being too high and causing the blood vessels to collapse. The vacuum pump 61 is also equipped with an anti-backflow plate on the connecting pipe). The negative pressure tube 66 creates a negative pressure in the disposable blood collection bottle 7, so that the blood collection needle 8 opens to draw blood from the rat's aorta. The blood enters the disposable blood collection bottle 7 for storage. The experimenter uses the scale 71 to determine the amount of blood drawn (i.e., 7-8 ml of blood is needed for normal syringe blood collection). Once the value is reached, the vacuum pump 61 is stopped by the controller 9. The operation is relatively convenient.

[0046] After completion, press down on the disposable blood collection bottle 7 to separate its connecting plug 72 from the connecting sleeve 65, remove the disposable blood collection bottle 7 for use, and then install a new disposable blood collection bottle 7. It is relatively convenient.

Claims

1. An experimental rat abdominal aortic fixation and blood collection device, comprising an experimental body (1), characterized in that: The experimental body (1) is equipped with an operating table (12) at the top, and the operating table (12) has a Velcro structure in the middle and an absorbent pad (3) is attached thereto. Four side plates (2) are vertically fixed at the four corners of the experimental body (1), and supplementary lights (21) are installed on the opposite sides of the four side plates (2). Four moving slots (11) are opened near the four corners of the operating table (12), and four magnetic binding devices (4) are movably connected in the four moving slots (11). A controller (9) is fixed at the front end of the experimental body (1) and is electrically connected to an external power source. A negative pressure extraction mechanism (6) is installed and fixed on the side of the experimental body (1). A blood collection needle (8) is connected through the top of the negative pressure extraction mechanism (6), and a disposable blood collection bottle (7) is embedded in the middle of the negative pressure extraction mechanism (6). The magnetic restraint device (4) includes a moving block (42) and a moving box (43). The moving block (42) is movably installed in the moving slot (11), and two through slots (421) are opened on both sides of the moving block (42), and the two ends of the elastic restraint strap (41) are inserted therein. The moving box (43) is fixed to the bottom of the operating table (12), and two iron blocks (411) are set inside the moving box (43) and fixed separately at the two ends of the elastic restraint strap (41). An electromagnet (44) is fixedly installed at the bottom of the moving box (43). The negative pressure extraction mechanism (6) includes a vacuum pump (61) and a mounting base (62). The mounting base (62) is fixed to the side of the experimental body (1), and an inlay groove (63) is provided on one side of the mounting base (62). A connecting sleeve (65) is fixed at the top of the inlay groove (63), and one end of a negative pressure tube (66) and a delivery tube (641) are respectively connected through the inside of the connecting sleeve (65). The delivery tube (641) passes through the top of the inlay groove (63) and is provided with a delivery head (64) that is connected to the blood collection needle (8). The other end of the negative pressure tube (66) is connected to the negative pressure interface of the vacuum pump (61), and the vacuum pump (61) is fixedly installed inside the experimental body (1). An embedding groove (631) is provided at the bottom of the inlay groove (63), and a spring (633) is fixedly connected at the bottom of the embedding groove (631). A stop plate (632) is fixedly connected at the top of the spring (633).

2. The experimental rat abdominal aorta fixation and blood collection device according to claim 1, characterized in that, The internal length of the movable box (43) is equal to the length of the movable slot (11).

3. The experimental rat abdominal aorta fixation and blood collection device according to claim 1, characterized in that, The movable block (42) is installed in the movable slot (11) and has an I-shaped cross section.

4. The experimental rat abdominal aorta fixation and blood collection device according to claim 1, characterized in that, The disposable blood collection bottle (7) is made of transparent PET material, and a scale (71) is provided on the outer surface of the disposable blood collection bottle (7). A connecting plug (72) is connected through the top of the disposable blood collection bottle (7), and a rubber ring gasket (73) is sleeved on the outside of the connecting plug (72).

5. The experimental rat abdominal aorta fixation and blood collection device according to claim 4, characterized in that, The outer diameter of the connector (72) is equal to the inner diameter of the connector sleeve (65), and the outer diameter of the disposable blood collection bottle (7) is equal to the inner diameter of the embedding groove (631).

6. The experimental rat abdominal aorta fixation and blood collection device according to claim 1, characterized in that, The disposable blood collection bottle (7) is inserted into the embedding groove (631), and the rubber gasket (73) is embedded in the connecting sleeve (65), and the connecting sleeve (65) and the rubber gasket (73) are in contact and sealed.

7. The experimental rat abdominal aorta fixation and blood collection device according to claim 1, characterized in that, The controller (9) is electrically connected to four fill lights (21), four electromagnets (44) and a vacuum pump (61).

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