Bumblebee cold tolerance gene and uses thereof
By mining the cold-resistant genes LOC100649067 and LOC100644218 of the Asian ground bumblebee, recombinant DNA was constructed and applied to the ground bumblebee, solving the problem of the ground bumblebee not functioning at low temperatures and achieving the effect of improving pollination rate and fruit and vegetable yield.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- HENGSHUI WOFENG BIOTECHNOLOGY CO LTD
- Filing Date
- 2022-09-07
- Publication Date
- 2026-05-12
AI Technical Summary
In early spring when temperatures are low, ground bumblebees are reluctant to leave their hives to work, resulting in low pollination rates for crops and affecting crop yields.
By mining cold-resistant genes, particularly LOC100649067 and LOC100644218, from Asian ground bumblebee populations, recombinant DNA was constructed and inserted into an expression vector for breeding cold-resistant ground bumblebees.
The cold-resistant bumblebees cultivated can still provide pollination services under low-temperature conditions, increase fruit and vegetable yields, and facilitate the screening and identification of cold-resistant varieties.
Smart Images
Figure CN116179559B_ABST
Abstract
Description
Technical Field
[0001] This invention belongs to the field of biotechnology, specifically relating to the cold-resistant gene of the ground bumblebee and its application. Background Technology
[0002] Bumblebees are a collective term for insects in the genus Bombus of the family Apidae, and they are important pollinators.
[0003] There are currently about 250 species of bumblebees worldwide. The ground bumblebee (Bombus terrestris) is one such species. Originating in Europe, ground bumblebees have been successfully domesticated and can provide excellent pollination services for greenhouse crops, thus finding widespread use worldwide, including in my country.
[0004] One current problem in agricultural production is that during the early spring when temperatures are low, the ground bumblebees currently used for crop pollination rarely leave their hives to work, thus affecting the pollination rate and directly impacting crop yields. Therefore, developing cold-resistant ground bumblebee varieties has become an urgent issue to address. Summary of the Invention
[0005] Purpose of the invention: This invention addresses the problems existing in the prior art by disclosing the cold-resistant gene of the ground bumblebee and its application.
[0006] Technical solution: The cold-resistant gene of the ground bumblebee has the nucleotide sequence shown in SEQ No. 1.
[0007] The cold-resistance gene of the ground bumblebee has the nucleotide sequence shown in SEQ No. 2.
[0008] A recombinant DNA containing the aforementioned cold-resistance gene from the ground bumblebee.
[0009] An expression vector, into which the above-mentioned recombinant DNA is inserted, uses microbial, animal, or plant cells as host cells.
[0010] The application of the above-mentioned cold-resistant genes of ground bumblebees in the breeding of cold-resistant ground bumblebees.
[0011] Beneficial effects: The cold-resistant gene of the ground bumblebee disclosed in this invention and its application have the following beneficial effects:
[0012] 1. It is beneficial to cultivate cold-resistant bumblebee varieties, enabling bumblebees to still provide pollination services when temperatures are low, thereby increasing fruit and vegetable yields;
[0013] 2. It is beneficial for the screening and identification of cold-resistant bumblebee varieties. Attached Figure Description
[0014] Figures 1a to 1cThis diagram illustrates a temperature comparison between the Asian and European habitats of ground bumblebees. It shows that the temperature in the Asian habitat is significantly lower than that in the European habitat.
[0015] Figure 2 This diagram illustrates the natural selection process of the LOC100649067 gene in Asian populations of the ground bumblebee. It shows that the LOC100649067 gene has a high Fst value and a low pi value.
[0016] Figure 3 This diagram illustrates the natural selection process of the LOC100644218 gene in Asian populations of the ground bumblebee. It shows that the LOC100644218 gene has a high Fst value and a low pi value. Detailed implementation method:
[0017] The specific embodiments of the present invention are described in detail below.
[0018] Wild ground bumblebees are widely distributed throughout the world, from Europe in the west to Xinjiang in my country in the east. We collected 55 wild ground bumblebee samples from Europe and Asia.
[0019] Climate data from these bumblebee sampling sites were downloaded from the Worldclim database (https: / / www.worldclim.org / ) using ArcMap (v10.2) software, and statistical analysis was performed using the Wilcoxon rank-sum test. Figures 1a to 1c As shown, the living temperature of ground bumblebees in Asia is significantly lower than that of ground bumblebees in Europe, which suggests that the genome of ground bumblebees living in Asia should contain cold-resistant genes.
[0020] In order to discover the cold-resistant genes contained in the bumblebee populations in Asia:
[0021] First, we performed genome resequencing on 55 ground bumblebee samples from Asia and Europe (sequences stored in GenBank: SRP311478).
[0022] Then, the sequencing results were compared with the reference genome sequence of ground bumblebee (GenBank:GCA_000214255.1) using BWA software, and the comparison results were analyzed using VCFtools software to calculate the Fst value and pi value of the two populations.
[0023] Finally, the selected genes were identified in the Asian population of ground bumblebees.
[0024] The intersection of the top 5% of Fst values and the bottom 5% of pi ratios (European / Asian populations) represents the genes selected in the Asian population, which are associated with the adaptation of ground bumblebees to their Asian habitat.
[0025] We used KOBAS software to perform functional annotation on the identified selected genes and screened for genes related to cold resistance. Glycerol phospholipids (GPL) are very important for the cold resistance of insects. We identified two genes, LOC100649067 (gene shown in SEQ NO. 1) and LOC100644218 (gene shown in SEQ NO. 2), which are genes in the glycerol phospholipid metabolic pathway. These genes were found in Asian populations of ground bumblebees, i.e., populations with low habitat temperatures (e.g., ...). Figures 1a to 1c As shown), they have all been subject to obvious natural selection (e.g. Figure 2 , Figure 3 As shown in the image, this indicates that these two genes are related to the cold resistance of ground bumblebees, among which:
[0026] The LOC100649067 gene of the ground bumblebee is located on chromosome 10 of the ground bumblebee (GenBank sequence number NC_015771.1), from position 1768246 to position 1783079.
[0027] The LOC100644218 gene of the ground bumblebee is located on chromosome 11 of the ground bumblebee (GenBank sequence number NC_015772.1), from position 15152441 to position 15189862.
[0028] The embodiments of the present invention have been described in detail above. However, the present invention is not limited to the above embodiments, and various changes can be made within the scope of knowledge possessed by those skilled in the art without departing from the spirit of the present invention.
Claims
1. Application of the cold-resistant gene of ground bumblebee in the breeding of cold-resistant ground bumblebees, wherein the cold-resistant gene of ground bumblebee has the nucleotide sequence shown in SEQ No.1 or SEQ No.2.