A solid longicorn bait and preparation method thereof

Abstract

The present invention relates to a solid longhorn beetle attractant core and a preparation method thereof. The attractant core comprises an attractant and a carrier made of sawdust, wherein the attractant is adsorbed in the carrier; the attractant comprises borneol acetate, nonanal and ethanol, and the weight ratio of borneol acetate, nonanal and ethanol is (1-100): (10-50): (10-50). The preparation method comprises: configuration of attractant stock solution, configuration of attractant reserve solution, carrier preparation and attractant adsorption. The present invention optimizes the attractant formula, has a good attracting effect on longhorn beetles, is conducive to sawdust adsorption and sustained release, and prolongs its effective period. By adsorbing the attractant through a solid carrier, it can be more convenient to install, transport and store, is not prone to problems such as penetration and breakage, and is less likely to cause flammable hazards. Moreover, the carrier is made of sawdust, is easy to obtain, has low cost, and is waste-utilized, which effectively solves the problem of sawdust treatment. In addition, the present invention has a simple process and is easy to prepare.

Description

Technical Field

[0001] The invention relates to the technical field of insect control, and in particular to a solid longicorn attractant and a preparation method thereof. Background Art

[0002] Pine alternator beetle (Monlchamus alternatus Hope) belongs to the order Coleoptera, family Cerambycidae, subfamily Cerambylinae, genus Monlchamus. Pine alternator beetles not only damage weaker trees with their larvae by boring into them, destroying the xylem and phloem of trees, leading to insufficient or interrupted water and nutrient transport, seriously affecting the growth of pine trees, and even causing large tracts of pine trees to die. What’s more serious is that the adult pine alternator is also the main vector of the devastating disease of pine trees - pine wilt disease (Bursaphelenchus xylophilus), which is called "cancer of pine trees" by researchers around the world. Since pine wilt disease was first discovered on black pines in the Sun Yat-sen Mausoleum in Nanjing in 1982, China has lost more than 5 million m of timber. 3 , resulting in direct economic losses of about 2.5 billion yuan. Pine wilt disease has destroyed my country's natural landscape and forest ecological environment, posed a serious threat to the sustainable and healthy development of my country's forestry, and caused significant losses to the national economy and people's lives. Preventing and controlling pine sawyer beetles and cutting off the transmission vectors of pine sawyer disease have become the key to preventing and controlling pine sawyer disease. At present, the control of pine wilt disease mainly adopts methods such as clearing dead trees, spraying chemical pesticides and attractant control. Among them, attractant technology has the characteristics of being green, environmentally friendly and efficient, and has become one of the most commonly used methods for preventing and controlling adult pine sawyer beetles.

[0003] Existing longhorn beetle attractants are basically in the form of liquid and used. Specifically, they can be divided into two methods of use: one is to directly contain them in a slow-release bottle for use; the other is to absorb the attractant through absorbent cotton and then heat-seal the absorbent cotton in a polyethylene bag (or other slow-release bag) for use. Among the above methods, the latter is currently used more often, because the slow-release bag has a relatively good slow-release effect and a long lasting effect, and because the attractant is usually used in large quantities in pine forests, it is relatively easy to carry and replace the slow-release bagged attractant, and the abandoned slow-release bag is also easier to collect and process. However, there are also problems in the method of containing the attractant in a slow-release bag. First, if the storage time is long, some components of the attractant will corrode the slow-release bag. In addition, the slow-release bag is easily damaged due to long-distance transportation and backlog, and most of the slow-release agents contain ethanol, which is easy to cause flammable hazards. Summary of the invention

[0004] The object of the present invention is to provide a solid longicorn attractant core and a preparation method thereof, which has good attracting effect, is convenient to use, transport and store, is not prone to cause flammable hazards, and has low cost.

[0005] To achieve the above-mentioned purpose, the present invention discloses a solid longhorn beetle attractant core, which includes an attractant and a carrier made of wood chips, wherein the attractant is adsorbed in the carrier; the attractant includes borneol acetate, nonanal and ethanol, and the weight ratio of borneol acetate, nonanal and ethanol is (1-100): (10-50): (10-50).

[0006] Preferably, the attractant further comprises a photostabilizer, and the amount of the photostabilizer used is 0.5-2% of the total weight of borneol acetate, nonanal and ethanol.

[0007] Preferably, the light stabilizer comprises at least one of a benzophenone ultraviolet absorber, a triazole ultraviolet absorber, a substituted acrylonitrile ultraviolet absorber, a triazine ultraviolet absorber and a hindered amine ultraviolet absorber.

[0008] Preferably, the carrier further comprises a binder, and the amount of the binder is 1-2% of the weight of the sawdust.

[0009] Preferably, the adhesive comprises at least one of starch syrup, syrup, acacia gum syrup and cellulose derivatives.

[0010] Preferably, the wood chips are pine wood chips.

[0011] Preferably, the device further comprises a sustained-release bag for sealing and packaging the carrier.

[0012] Preferably, the sustained-release bag is a paper fiber sustained-release bag.

[0013] The present invention also discloses a method for preparing a solid longicorn attractant core, which comprises the following steps:

[0014] Preparation of attractant stock solution: Mix borneol acetate, nonanal and ethanol in a weight ratio of (1-100): (10-50): (10-50) to obtain an attractant stock solution.

[0015] Preparation of attractant reserve solution: add 0.5-2% of the light stabilizer by weight of the attractant stock solution to the attractant stock solution and mix them to obtain the attractant reserve solution.

[0016] Preparation of the carrier: drying the sawdust, adding 1-2% of the weight of the sawdust as an adhesive to the dried sawdust, mixing the sawdust and the adhesive evenly, and then compressing and molding to obtain the carrier.

[0017] Attractant adsorption: Soak the carrier in the attractant reserve solution, take it out after soaking, and use absorbent paper to absorb the attractant reserve solution remaining on the surface of the carrier; then put the carrier into a slow-release bag and seal it to obtain the attractant core.

[0018] Preferably, the carrier is immersed in the attractant for 2 to 4 hours.

[0019] The present invention has the following beneficial effects:

[0020] The present invention optimizes the attractant formula, has a good attractant effect on longhorn beetles, is conducive to wood chip adsorption and slow release, and prolongs its effective period. By adsorbing the attractant on a solid carrier, it is more convenient to install, transport and store, is not prone to problems such as penetration and breakage, and is less likely to cause flammable hazards. Moreover, the carrier is made of wood chips, which is easy to obtain, low in cost, and waste is utilized, effectively solving the problem of wood chip treatment. In addition, the present invention has a simple process and is easy to prepare. DETAILED DESCRIPTION

[0021] In order to make the purpose, technical solutions and advantages of the present invention more clearly understood, the present invention is further described in detail below in conjunction with embodiments.

[0022] The present invention discloses a solid longhorn beetle attractant core, which includes an attractant, a carrier and a slow-release bag. The attractant is adsorbed in the carrier, and the slow-release bag seals and wraps the carrier adsorbed with the attractant. In this case, the carrier is a sawdust block made of compressed sawdust, and its specifications are 4-10cm×2-6cm×0.5-2cm. The sawdust is preferably pine sawdust, which can be obtained by collecting debris from wood processing in the forest, recycling waste, and has low cost and wide sources. In order to prevent the compressed sawdust block (carrier) from becoming loose, an adhesive can be mixed with the sawdust and then compressed, and the amount of the adhesive is 1-2% of the weight of the sawdust. The adhesive is selected from one or more of starch slurry, syrup, gum arabic slurry and cellulose derivatives. These adhesives are easy to decompose naturally and are beneficial to environmental protection.

[0023] The slow-release bags are made of paper fiber slow-release bags, which are conducive to the distribution of attractants. At the same time, the packaging is low-carbon and environmentally friendly and will not cause secondary white pollution in the forest.

[0024] The attractant comprises borneol acetate, nonanal, ethanol and a light stabilizer, wherein the weight ratio of borneol acetate, nonanal and ethanol is (1-100): (10-50): (10-50), and the amount of the light stabilizer is 0.5-2% of the total weight of borneol acetate, nonanal and ethanol. The light stabilizer is one or more of benzophenone ultraviolet absorbers, triazole ultraviolet absorbers, substituted acrylonitrile ultraviolet absorbers, triazine ultraviolet absorbers and hindered amine ultraviolet absorbers. By adding the light stabilizer, the effective cost of the attractant can be ensured not to be easily decomposed by light.

[0025] The present invention also discloses a method for preparing the solid longicorn attractant core, which comprises the following steps:

[0026] Preparation of attractant stock solution: Mix borneol acetate, nonanal and ethanol in a weight ratio of (1-100): (10-50): (10-50) to obtain an attractant stock solution.

[0027] Preparation of attractant reserve solution: add 0.5-2% of the light stabilizer by weight of the attractant stock solution to the attractant stock solution and mix them to obtain the attractant reserve solution.

[0028] Preparation of the carrier: drying the sawdust, adding 1-2% of the weight of the sawdust as an adhesive to the dried sawdust, mixing the sawdust and the adhesive evenly, and then compressing and molding to obtain the carrier.

[0029] Attractant adsorption: Soak the carrier in the attractant reserve solution for 2 to 4 hours. Take it out after soaking, use absorbent paper to absorb the attractant reserve solution remaining on the surface of the carrier, and then put the dried carrier into a slow-release bag and seal it to obtain the attractant core. Put the attractant core in the outer package and seal it for storage or transportation.

[0030] The following specific tests are performed to verify the use effect of the present invention.

[0031] Test 1:

[0032] The test site is located in the pine wood nematode disease outbreak area of ​​Xishan Bantou Forest Farm, Tong'an District, Xiamen City (24°43′50″N, 117°59′28″E), the forest is pine and broad-leaved forest, 40% pine trees, 400-536m above sea level, 45° average slope, and red soil of brick red soil. There are ten groups in the test, each with three samples, which are loaded into 30 identical traps. The corresponding components and dosages of the attractant stock solutions of the ten groups of tests are shown in Table 1. In each group of samples, the dosage of the light stabilizer (using hindered amine ultraviolet absorber) is 1% of the weight of the attractant stock solution, and the same specifications (5cm×4cm×2cm) of sawdust carriers are used for adsorption, and the carriers are wrapped and sealed with paper fiber slow-release bags. Each trap was hung 1.5 to 2.0 m above the ground. The traps were randomly arranged, and the distance between each trap was not less than 50 m. A relatively open and well-ventilated place in the forest was selected as the hanging point of the trap. The test time was from June 1, 2022 to August 1, 2022. The number of Monochamus alternatus in the insect collection bottle was recorded every 7 days and the debris in the insect collection bottle was cleaned. The test results are shown in Table 2.

[0033] Table 1

[0034] Sample Attractant stock solution formula A1 Bornyl acetate 25mg + nonanal 15mg + hindered amine UV absorber 0.4mg A2 Nonanal 24mg + ethanol 26mg + hindered amine UV absorber 0.4mg A3 Bornyl acetate 29mg + ethanol 11mg + hindered amine UV absorber 0.4mg A4 Bornyl acetate 40mg + hindered amine UV absorber 0.4mg A5 Bornyl acetate 20mg + nonanal 12mg + ethanol 8mg + hindered amine UV absorber 0.4mg A6 Bornyl acetate 20mg + nonanal 10mg + ethanol 10mg + hindered amine UV absorber 0.4mg A7 Bornyl acetate 4mg + nonanal 12mg + ethanol 24mg + hindered amine UV absorber 0.4mg A8 Bornyl acetate 12mg + nonanal 20mg + ethanol 8mg + hindered amine UV absorber 0.4mg A9 Bornyl acetate 10mg + nonanal 20mg + ethanol 10mg + hindered amine UV absorber 0.4mg A10 Bornyl acetate 10mg + nonanal 10mg + ethanol 20mg + hindered amine UV absorber 0.4mg

[0035] Note: The weight of each component in Table 1 is calculated as follows: first weigh the unsoaked carrier to obtain data 1, then weigh the carrier after soaking and wiping off the residual liquid on the surface to obtain data 2, and subtract data 1 from data 2 to get the total weight of the attractant reserve solution, and then calculate the weight of each component according to the corresponding ratio of the attractant reserve solution.

[0036] Table 2

[0037]

[0038] The results of experiment 1 showed that the formulas with borneol acetate had a good attracting effect on the adults of Monochamus alternatus, while the formula without borneol acetate (A2) had the worst effect. Therefore, it was inferred that borneol acetate was an important synergistic substance for attracting the adults of Monochamus alternatus, and the combination of borneol acetate with other ingredients could significantly improve the attracting activity. At the same time, the results also showed that the attracting effects of formulas A3 and A4 without nonanal on the adults of Monochamus alternatus were slightly worse than those of other formulas with nonanal, indicating that nonanal played an important role in improving the attracting effect of the adults of Monochamus alternatus. The results of experiment 1 showed that the different mass ratios of borneol acetate, nonanal and ethanol had a significant effect on the trapping results. Among them, formula A5, i.e., the ratio of borneol acetate, nonanal and ethanol was 5:3:2, had the highest trapping effect, indicating that the optimal mass ratio of borneol acetate, nonanal and ethanol was 5:3:2.

[0039] Test 2:

[0040] The test site is located in the pine wood nematode disease outbreak area of ​​Xishan Bantou Forest Farm, Tong'an District, Xiamen City (24°43′50″N, 117°59′28″E). The forest is a pine broad-leaved forest with 40% pine trees, an altitude of 400-536m, an average slope of 45°, and brick red soil. There are 3 groups in the test, each with 3 groups of samples, which are loaded into 9 identical traps. The same attractant was used in all 3 groups of experiments, but the slow-release carriers were different, as shown in Table 3. Each trap was hung 1.5-2.0m from the ground, and the traps were randomly arranged, and the distance between the two traps was not less than 50m. A relatively open and well-ventilated place in the forest was selected as the hanging point of the trap. The test time was from June 1, 2022 to September 1, 2022. The number of pine sawyer beetles in the insect collecting bottle was recorded every 7 days and the debris in the insect collecting bottle was cleaned. The test results are shown in Table 4.

[0041] Table 3

[0042]

[0043] Note: In this test, sample B3 is prepared first. The preparation of sample B3 is as follows: first weigh the unsoaked carrier to obtain data 1, and then weigh the carrier after soaking and wiping off the residual liquid on the surface to obtain data 2. Subtract data 1 from data 2 to obtain the total weight of the attractant reserve solution. According to the ratio of the attractant reserve solution, the weight of each component can be calculated accordingly. Finally, take the same amount of the same attractant reserve solution as sample B3 and place it in other carriers in Table 3 above to obtain samples B1 and B2.

[0044] Table 4

[0045]

[0046] The results of the second experiment showed that the wood chip carrier had the best effect on attracting pine beetles and had a longer lasting effect. Other materials had poorer effects.

[0047] Test 3:

[0048] The test site is located in the pine wood nematode disease outbreak area of ​​Xishan Bantou Forest Farm, Tong'an District, Xiamen City (24°43′50″N, 117°59′28″E). The forest is a pine and broad-leaved forest, with 40% pine trees, an altitude of 400-536m, an average slope of 45°, and brick-red soil. There are 3 groups in the test, each with 3 groups of samples, which are loaded into 9 identical traps. The same attractant was used in all 3 groups of experiments, and the attractant was adsorbed with sawdust carriers of the same specifications, but different slow-release bags were selected, see Table 5 for details. Each trap is hung 1.5-2.0m from the ground, and the traps are randomly arranged, and the distance between each trap is not less than 50m. A relatively open and well-ventilated place in the forest is selected as the hanging point of the trap. The test period was from June 1, 2022 to September 1, 2022. The number of Monochamus alternatus in the insect collection bottles was recorded every 7 days and the debris in the insect collection bottles was cleaned. The test results are shown in Table 6.

[0049] Table 5

[0050]

[0051] Note: The dosage of each component of the attractant is obtained in the same way as in Experiment 1.

[0052] Table 6

[0053]

[0054] The results of Experiment 3 showed that different slow-release bag materials had a significant impact on the trapping results. The trapping effect of the paper fiber bag treatment was higher than that of the other two treatments, both in terms of the number of traps and the duration of effectiveness in the forest.

[0055] Test 4:

[0056] The test site is located in the pine wood nematode disease outbreak area of ​​Xishan Bantou Forest Farm, Tong'an District, Xiamen City (24°43′50″N, 117°59′28″E). The forest is divided into pine and broad-leaved forests, with 40% pine trees, an altitude of 400-536m, an average slope of 45°, and brick-red soil. There are 3 groups in the test, each with 3 groups of samples, which are loaded into 9 identical traps respectively. The purpose of Test 4 is to compare the solid longhorn beetle attractant core of the present invention with two similar products (liquid type) on the market. The attractant content of the three groups of samples is equal. Among them, the attractant formula of the solid longhorn beetle attractant core of the present invention is: 20 mg of borneol acetate + 12 mg of nonanal + 8 mg of ethanol + 0.4 mg of benzophenone ultraviolet absorber, see Table 7 for details. Each trap was hung 1.5 to 2.0 m above the ground. The traps were randomly arranged, and the distance between each trap was not less than 50 m. A relatively open and well-ventilated place in the forest was selected as the hanging point of the trap. The test time was from June 1, 2022 to September 1, 2022. The number of Monochamus alternatus in the insect collection bottle was recorded every 7 days and the debris in the insect collection bottle was cleaned. The test results are shown in Table 7.

[0057] Table 7

[0058]

[0059] The results of the fourth test showed that the solid longhorn beetle attractant core attracted more longhorn beetles than the pine beetle attractant I on the market, but the difference with the pine beetle attractant II was not obvious. However, the solid longhorn beetle attractant core had a relatively long lasting effect when used in the forest. Since the other two attractants are liquid, during long-distance transportation and storage in warehouses, the backlog of goods is prone to leakage, which can easily lead to the hidden danger of product flammability.

[0060] The above description is only a preferred specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any changes or substitutions that can be easily conceived by any technician familiar with the technical field within the technical scope disclosed by the present invention should be covered within the protection scope of the present invention.

Claims

1. A solid longicorn bait, characterized in that: The invention comprises an attractant and a carrier made of sawdust, wherein the attractant is adsorbed in the carrier; the attractant comprises borneol acetate, nonanal and ethanol, and the weight ratio of borneol acetate, nonanal and ethanol is 5:3:

2.

2. The solid beetle attractant according to claim 1, characterized in that: The attractant also includes a photostabilizer, and the amount of the photostabilizer is 0.5-2% of the total weight of borneol acetate, nonanal and ethanol.

3. The solid longicorn attractant according to claim 2, characterized in that: The light stabilizer comprises at least one of a benzophenone ultraviolet absorber, a triazole ultraviolet absorber, a substituted acrylonitrile ultraviolet absorber, a triazine ultraviolet absorber and a hindered amine ultraviolet absorber.

4. The solid longicorn attractant according to claim 1, characterized in that: The carrier also includes a binder, and the amount of the binder is 1-2% of the weight of the sawdust.

5. The solid longicorn attractant according to claim 4, characterized in that: The adhesive comprises at least one of starch slurry, syrup, acacia gum slurry and cellulose derivatives.

6. The solid longicorn attractant according to claim 1, characterized in that: The sawdust is pine sawdust.

7. The solid longicorn attractant according to claim 1, characterized in that: A sustained release bag for sealing the package carrier is also included.

8. The solid longicorn attractant according to claim 7, characterized in that: The sustained-release bag is a paper fiber sustained-release bag.

9. A method for preparing a solid longicorn bait core, characterized in that: The following steps are involved: Preparation of attractant stock solution: Mix borneol acetate, nonanal and ethanol in a weight ratio of 5:3:2 to obtain an attractant stock solution; Preparation of attractant reserve solution: adding 0.5-2% of the weight of the attractant stock solution of the light stabilizer to the attractant stock solution and mixing, to obtain the attractant reserve solution; Preparation of carrier: drying sawdust, adding 1-2% of the weight of sawdust adhesive to the dried sawdust, mixing the sawdust and adhesive evenly, and then compressing and molding to obtain a carrier; Attractant adsorption: Soak the carrier in the attractant reserve solution, take it out after soaking, and use absorbent paper to absorb the attractant reserve solution remaining on the surface of the carrier; then put the carrier into a slow-release bag and seal it to obtain the attractant core.

10. The method for preparing the solid longicorn bait according to claim 9, characterized in that: The carrier is immersed in the attractant for 2 to 4 hours.

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