7-(Piperidin-1-yl)-4H-pyrimido[1,2-B]pyridazin-4-one derivatives as positive allosteric modulators of muscarinic acetylcholine receptor M4

Abstract

Disclosed herein are analogs of 6-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl)oxy)piperidin-1-yl)-[1,2,4]triazolo[4,3-b]pyridazine, i.e., 7-(4-((phenyl or pyridin-3-yl)oxy)piperidin-1-yl)-4H-pyrimido[1,2-b]pyridazin-4-one derivatives of formula (I), which are useful as positive allosteric modulators of muscarinic acetylcholine receptor M4 (mAChR M4). Also disclosed herein are methods of preparing the compounds, pharmaceutical compositions comprising the compounds, and methods of using the compounds and compositions to treat neurological and psychiatric disorders associated with muscarinic acetylcholine receptor dysfunction.

Description

[0001] Related Applications

[0002] This application claims the benefit of priority to U.S. Application Serial No. 63 / 052,085, filed on July 15, 2020, which is incorporated herein by reference in its entirety. Technical Field

[0003] The present invention relates to compounds, compositions, and methods for treating neurological and psychiatric disorders associated with muscarinic acetylcholine receptor dysfunction. Background Art

[0004] Cholinergic neurotransmission involves the activation of nicotinic acetylcholine receptors (nAChR) or muscarinic acetylcholine receptors (mAChR) by the binding of the endogenous orthosteric agonist acetylcholine (ACh). Conditions associated with cognitive impairment, such as Alzheimer's disease, are accompanied by a decrease in the amount of acetylcholine in the brain. This is thought to be the result of degeneration of basal forebrain cholinergic neurons, which extensively innervate multiple areas of the brain, including the association cortex and hippocampus, which play a key role in higher processes. Clinical data support that cholinergic hypofunction contributes to cognitive deficits in patients with schizophrenia. Efforts to increase acetylcholine levels have focused on increasing choline levels (a precursor for acetylcholine synthesis) and blocking acetylcholinesterase (AChE), an enzyme that metabolizes acetylcholine. Therefore, acetylcholinesterase (AChE) inhibitors that inhibit the hydrolysis of ACh have been approved in the United States for palliative treatment of cognitive deficits in AD patients, but not for disease-modifying treatment thereof.

[0005] Attempts to enhance central cholinergic function by administering choline or phosphatidylcholine have not been successful. AChE inhibitors have shown therapeutic efficacy, but have been found to have frequent cholinergic side effects due to peripheral acetylcholine stimulation, including abdominal cramps, nausea, vomiting, and diarrhea. These gastrointestinal side effects have been observed in about one-third of treated patients. In addition, some AChE inhibitors such as tacrine have been found to cause significant hepatotoxicity, with elevated liver transaminases observed in about 30% of patients. The side effects of AChE inhibitors have severely limited their clinical application. An alternative approach to pharmacologically targeting cholinergic hypofunction is to activate mAChRs, which are widely expressed throughout the body.

[0006] mAChR is a member of the G protein-coupled receptor (GPCR) family A and includes five subtypes, designated M1-M5. The M1, M3, and M5 subtypes mainly interact with G q Couples to and activates phospholipase C, while the M2 and M4 isoforms primarily bind to G i / oThe mAChR subtypes are coupled to the relevant effector systems. These five different mAChR subtypes have been identified in the mammalian central nervous system, where they are common and differentially expressed. M1-M5 have different roles in cognition, sensation, movement and autonomic function. Therefore, without wishing to be bound by a particular theory, it is believed that selective agonists of mAChR subtypes that regulate processes involved in cognitive function may prove to be excellent therapies for the treatment of psychosis, schizophrenia and related disorders. The muscarinic M4 receptor has been shown to play an important role in cognitive processes and is believed to play an important role in the pathophysiology of mental disorders including schizophrenia.

[0007] There is evidence that the most significant side effects of AChE inhibitors and other cholinergic agents are mediated by activation of peripheral M2 and M3 mAChRs and include bradycardia, gastrointestinal discomfort, excessive salivation and sweating. In contrast, M4 is considered to be the subtype most likely to mediate the effects of muscarinic acetylcholine receptor dysfunction in psychiatric disorders including schizophrenia, cognitive impairment and neuropathic pain. Therefore, a lot of efforts have been focused on developing selective M4 agonists for the treatment of these disorders. Unfortunately, these efforts have been mostly unsuccessful due to the inability to develop compounds that are highly selective for mAChR M4. Therefore, mAChR agonists that have been tested in clinical studies induce a series of side effects by activating peripheral mAChRs. In order to fully understand the physiological effects of individual mAChR subtypes and further explore the therapeutic utility of mAChR ligands in psychosis (including schizophrenia, cognitive impairment and other disorders), it may be important to develop compounds that are highly selective activators of mAChR M4 and other individual mAChR subtypes.

[0008] Previous attempts to develop agonists that are highly selective for a single mAChR subtype have failed because the orthosteric ACh binding site is highly conserved. In order to circumvent the problems associated with targeting the highly conserved orthosteric ACh binding site, it is believed that compounds that act at allosteric sites on mAChR have been developed, which are different from the orthosteric site and are not so highly conserved. This approach improves the development of selective ligands for a variety of GPCR subtypes. In the case of mAChR, the main goal is to develop allosteric ligands that selectively increase the activity of mAChR M4 or other mAChR subtypes. Allosteric activators can include allosteric agonists, whose site of action is different from the orthosteric site, directly activating receptors in the absence of ACh; and positive allosteric modulators (PAMs), which do not directly activate receptors, but enhance the activation of receptors by endogenous orthosteric agonists ACh. In addition, a single molecule may have both allosteric potentiator and allosteric agonist activity.

[0009] Recently, muscarinic agonists, including xanomeline, have been shown in animal models to have activity similar to known antipsychotics, but without causing catalepsy (Bymaster et al., Eur. J. Pharmacol. 1998, 356, 109, Bymaster et al., Life Sci. 1999, 64, 527; Shannon et al., J. Pharmacol. Exp. Ther. 1999, 290, 901; Shannon et al., Schizophrenia Res. 2000, 42, 249). Furthermore, xanomeline has been shown to reduce psychotic behavioral symptoms such as delusions, suspiciousness, vocal outbursts and hallucinations in Alzheimer's disease patients (Bodick et al., Arch. Neurol. 1997, 54, 465), however treatment-induced side effects, such as gastrointestinal effects, have severely limited the clinical utility of this compound.

[0010] WO 2018 / 118736 discloses 6,5-fused heteroaryl piperidine ethers which are allosteric modulators of the M4 muscarinic acetylcholine receptor.

[0011] Despite the progress in muscarinic acetylcholine receptor research, there is still a lack of potent, effective and selective M4 mAChR activators, as well as compounds that are effective in treating neurological and psychiatric disorders related to cholinergic activity and diseases involving muscarinic M4 receptors. Summary of the invention

[0012] In a first aspect, the present invention provides a compound having formula (I),

[0013]

[0014] or a pharmaceutically acceptable salt thereof, wherein:

[0015] X is CR 5a or N;

[0016] R 1 is selected from hydrogen, C1-C4-alkyl, C1-C4-haloalkyl, C1-C4-hydroxyalkyl, C3-C6-cycloalkyl, -L 1 -C3-C6-cycloalkyl, halogen, -L 1 OR a OR a ;

[0017] R 2 is selected from hydrogen, C1-C4-alkyl, C1-C4-haloalkyl, C3-C6-cycloalkyl, halogen, -L2 OR b , OR b 、NHR b and N(R b )2;

[0018] R 3 is selected from hydrogen, C1-C4-alkyl, C1-C4-haloalkyl, C3-C6-cycloalkyl, halogen, -L 3 OR c OR c ;

[0019] Each R 4 Independently selected from C1-C4-alkyl, OR d , -L 4 OR d and halogens;

[0020] Each R 5 independently selected from C1-C4-alkyl, C1-C4-haloalkyl, OR e , -L 5 OR e , 5-membered or 6-membered heteroaryl, phenyl, C3-C6-cycloalkyl and halogen; or

[0021] Two of the R 5 Together with the carbon atoms to which they are attached, they form a 5- to 8-membered fused monocyclic heterocyclic ring containing 1-2 heteroatoms independently selected from O, N and S, the fused monocyclic heterocyclic ring being optionally substituted by 1-4 R 6 replace;

[0022] R 5a is selected from hydrogen, C1-C4-alkyl, C1-C4-haloalkyl, C3-C6-cycloalkyl, OR e , -L 5 OR e and halogens;

[0023] Each R 5b independently selected from C1-C2-alkyl, C1-C2-fluoroalkyl, C3-C4-cycloalkyl, -OC1-C2-alkyl, -OC1-C2-fluoroalkyl, cyano and halogen;

[0024] Each R 6 independently selected from oxo, C1-C4-alkyl, OR f , -L 6 OR f and halogens;

[0025] R 7 is selected from hydrogen, C1-C4-alkyl, C1-C4-haloalkyl, C3-C6-cycloalkyl, halogen, -L2 OR b , OR b 、NHR b and N(R b )2;

[0026] o is 0, 1, or 2;

[0027] m is 0, 1, or 2;

[0028] n is 0, 1, or 2;

[0029] L 1 , L 2 , L 3 , L 4 , L 5 and L 6 is independently at each occurrence C1-C3-alkylene; and

[0030] R a , R b , R c , R d , R e and R f are independently selected at each occurrence from C1-C4-alkyl, C1-C4-haloalkyl and C3-C6-cycloalkyl, wherein both R b Together with the nitrogen atom to which they are attached they optionally form a 4- to 7-membered monocyclic heterocyclyl group which is optionally substituted with 1-4 substituents independently selected from halogen, C1-C4-alkyl and C1-C4-haloalkyl.

[0031] In another aspect, the present invention provides a method for treating a neurological and / or psychiatric disorder in a patient, the method comprising administering to a patient in need thereof a therapeutically effective amount of a compound of formula (I) or a pharmaceutically acceptable salt or pharmaceutical composition thereof.

[0032] In another aspect, the present invention provides a compound of formula (I), or a pharmaceutically acceptable salt thereof, or a pharmaceutical composition for use in treating a neurological and / or psychiatric disorder.

[0033] In another aspect, the present invention provides the use of a compound of formula (I), or a pharmaceutically acceptable salt thereof, or a pharmaceutical composition for the preparation of a medicament for treating neurological and / or psychiatric disorders. BRIEF DESCRIPTION OF THE DRAWINGS

[0034] Figure 1 Schematic diagram showing the ligand binding site in the muscarinic acetylcholine receptor, including the orthosteric site and the allosteric site. DETAILED DESCRIPTION

[0035] Disclosed herein are positive allosteric modulators (i.e., potentiators) of muscarinic acetylcholine receptor M4 (mAChR M4), methods of preparing the same, pharmaceutical compositions comprising the same, and methods of using the same to treat neurological and psychiatric disorders associated with muscarinic acetylcholine receptor dysfunction. The compounds include analogs of 6-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl)oxy)piperidin-1-yl)-[1,2,4]triazolo[4,3-b]pyridazine.

[0036] Human muscarinic acetylcholine receptor M4 (mAChR M4) is a 479 amino acid protein encoded by the CHRM4 gene. The molecular weight of the unglycosylated protein is about 54 kDa, and it is a transmembrane GPCR. As mentioned above, mAChR M4 is a member of the GPCR class A family or rhodopsin-like GPCRs, characterized by structural features similar to rhodopsin, such as seven transmembrane segments. The N-terminus of the muscarinic acetylcholine receptor faces the extracellular surface of the membrane, and the C-terminus is located on the cytoplasmic surface. The structural schematic of mAChR M4 is shown in Figure 1 As shown, the transmembrane segment is shown as a cylinder (spanning the lipid bilayer of the cell membrane). Figure 1 As shown, orthosteric binding of the natural ligand acetylcholine of mAChR is located within a pocket of the transmembrane segment.

[0037] Previous attempts to develop agonists that are highly selective for a single mAChR subtype have failed because the orthosteric ACh binding site is highly conserved. In order to circumvent the problems associated with targeting the highly conserved orthosteric ACh binding site, it is believed that compounds have been developed that act at allosteric sites on the mAChR that are distal to the orthosteric site and are not as highly conserved. Without wishing to be bound by a particular theory, it is believed that the compounds of the present invention and products of the disclosed methods bind to an allosteric site that is distinct from the orthosteric binding site. For example, Figure 1 As shown, the disclosed compounds can bind at the binding site.

[0038] 1. Definition

[0039] Unless otherwise defined, all technical terms and scientific terms used herein have the same meaning as those of ordinary skill in the art are generally understood. In the case of a conflict, this document (including definitions) shall prevail. Although methods and materials similar or equivalent to those described herein can be used in the practice or testing of the present invention, preferred methods and materials are described below. All publications, patent applications, patents and other references mentioned herein are incorporated herein by reference in their entirety. The materials, methods and examples disclosed herein are only illustrative and are not intended to be restrictive.

[0040] As used herein, the terms "comprise(s)", "include(s)", "having", "has", "can", "contain(s)" and variations thereof are intended to be open transitional phrases, terms or words that do not exclude the possibility of additional acts or structures. Unless the context clearly dictates otherwise, the singular forms "a", "an", and "the" include plural referents. The present invention also encompasses other embodiments that "comprise", "consist of", and "consist essentially of" the embodiments or elements presented herein, whether or not explicitly set forth.

[0041] The modifier "about" used in connection with a quantity is inclusive of the stated value and has the meaning dictated by the context and the understanding of those skilled in the art.

[0042] Definitions of specific functional groups and chemical terms are described in more detail below. For purposes of the present invention, chemical elements are identified according to the Periodic Table of the Elements, CAS version, Handbook of Chemistry and Physics, 75th ed., inside cover, and specific functional groups are generally defined as described therein. In addition, general principles of organic chemistry as well as specific functional moieties and reactivities are described in Organic Chemistry, Thomas Sorrell, University Science Books, Sausalito, 1999; Smith and March March's Advanced Organic Chemistry, 5th ed., John Wiley & Sons, Inc., New York, 2001; Larock, Comprehensive Organic Transformations, VCH Publishers, Inc., New York, 1989; Carruthers, Some Modern Methods of Organic Synthesis, 3rd ed., Cambridge University Press, Cambridge, 1987; the entire contents of each of these references are incorporated herein by reference.

[0043] As used herein, the term "alkoxy" refers to an alkyl group, as defined herein, attached to the parent molecular moiety through an oxygen atom. Representative examples of alkoxy include, but are not limited to, methoxy, ethoxy, propoxy, 2-propoxy, butoxy, and tert-butoxy.

[0044] As used herein, the term "alkyl" means a straight or branched saturated hydrocarbon chain containing from 1 to 10 carbon atoms. 1- "C6-alkyl" means a straight or branched hydrocarbon containing from 1 to 6 carbon atoms. The term "C1-C3-alkyl" means a straight or branched hydrocarbon containing from 1 to 3 carbon atoms. Representative examples of alkyl include, but are not limited to, methyl, ethyl, n-propyl, isopropyl, n-butyl, sec-butyl, isobutyl, tert-butyl, n-pentyl, isopentyl, neopentyl, n-hexyl, 3-methylhexyl, 2,2-dimethylpentyl, 2,3-dimethylpentyl, 4,4-dimethylpentan-2-yl, n-heptyl, n-octyl, n-nonyl and n-decyl.

[0045] As used herein, the term "alkenyl" means a straight or branched hydrocarbon chain containing at least one carbon-carbon double bond and from 2 to 10 carbon atoms.

[0046] As used herein, the term "alkoxyalkyl" refers to an alkoxy group, as defined herein, attached to the parent molecular moiety through an alkyl group, as defined herein. Alkoxyalkyl can be represented by the general formula LOR, where L can be C 1-3 -alkylene such as CH2 and R can be C 1-3 -alkyl. Representative examples may include, but are not limited to, -CH2OCH3, -CH2OCH2CH3, -CH2OCH2CH2CH3.

[0047] The term "alkoxyfluoroalkyl" as used herein, means an alkoxy group, as defined herein, appended to the parent molecular moiety through a fluoroalkyl group, as defined herein.

[0048] As used herein, the term "alkylene" refers to a saturated divalent group derived from a straight or branched hydrocarbon having 1 to 10 carbon atoms, for example, 2 to 5 carbon atoms. Representative examples of alkylene include, but are not limited to, -CH2-, -CH2CH2-, -CH2CH2CH2-, -CH2CH(CH3)CH2-, -CH2CH2CH2CH2-, -CH2CH(CH3)CH2CH2-, and -CH2CH2CH2CH2CH2-.

[0049] The term "alkylamino" as used herein, means at least one alkyl group, as defined herein, appended to the parent molecular moiety through an amino group, as defined herein.

[0050] The term "aminoalkyl" as used herein, means at least one amino group, as defined herein, appended to the parent molecular moiety through an alkylene group, as defined herein.

[0051] As used herein, the term "amino" refers to -NR x R y , where R x and R y can be hydrogen, alkyl, cycloalkyl, aryl, heteroaryl, heterocyclic, alkenyl or heteroalkyl, or R x and R y Together with the N to which they are attached, they can form a heterocyclic ring. In the case of an aminoalkyl group or any other moiety in which an amino group is attached to two other moieties, the amino group can be -NR x -, where R x It may be hydrogen, alkyl, cycloalkyl, aryl, heteroaryl, heterocycle, alkenyl, or heteroalkyl.

[0052] As used herein, the term "aryl" refers to a phenyl group, or a bicyclic or tricyclic fused ring system. A bicyclic fused ring system is exemplified by a phenyl group attached to the parent molecular moiety and fused to a phenyl group, a cycloalkyl group as defined herein, a heteroaryl group as defined herein, or a heterocycle as defined herein. A tricyclic fused ring system is exemplified by a phenyl group attached to the parent molecular moiety and fused to a bicyclic fused ring system as described herein. Representative examples of aryl groups include, but are not limited to, phenyl, naphthyl, anthracenyl, indolyl (e.g., 1H-indol-4-yl, 1H-indol-5-yl, 1H-indol-6-yl, and 1H-indol-7-yl), benzodioxolyl (e.g., benzo[d][1,3]dioxol-4-yl and benzo[d][1,3]dioxol-5-yl), chromanyl (e.g., chroman-5-yl, chroman-6-yl, chroman-7-yl, and chroman-8-yl), and tetrahydroquinolinyl (e.g., 1,2,3,4-tetrahydroquinolin-5-yl, 1,2,3,4-tetrahydroquinolin-6-yl, 1,2,3,4-tetrahydroquinolin-7-yl, and 1,2,3,4-tetrahydroquinolin-8-yl).

[0053] The term "cycloalkoxy" as used herein, means a cycloalkyl group, as defined herein, appended to the parent molecular moiety through an oxygen atom.

[0054] As used herein, the term "cycloalkyl" refers to a carbocyclic ring system containing three to ten carbon atoms, zero heteroatoms and zero double bonds. The cycloalkyl may be monocyclic, bicyclic, bridged, fused or spirocyclic. Representative examples of cycloalkyl include, but are not limited to, cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cycloheptyl, cyclooctyl, cyclononyl, cyclodecyl, adamantyl, and bicyclo[1.1.1]pentyl. "Cycloalkyl" also includes carbocyclic ring systems in which the cycloalkyl group is attached to the parent molecular moiety and fused to an aryl group as defined herein (e.g., a phenyl group), a heteroaryl group as defined herein, or a heterocycle as defined herein. Representative examples of such cycloalkyl groups include, but are not limited to, 2,3-dihydro-1H-indenyl (e.g., 2,3-dihydro-1H-inden-1-yl and 2,3-dihydro-1H-inden-2-yl), 6,7-dihydro-5H-cyclopenta[b]pyridinyl (e.g., 6,7-dihydro-5H-cyclopenta[b]pyridin-6-yl), oxaspiro[3.3]heptanyl (e.g., 2-oxaspiro[3.3]heptan-6-yl), and 5,6,7,8-tetrahydroquinolinyl (e.g., 5,6,7,8-tetrahydroquinolin-5-yl).

[0055] As used herein, the term "cycloalkenyl" refers to a non-aromatic monocyclic or polycyclic ring system containing at least one carbon-carbon double bond and preferably having from 5 to 10 carbon atoms per ring. The cycloalkenyl group may be monocyclic, bicyclic, bridged, fused or spirocyclic. Exemplary monocyclic cycloalkenyl rings include cyclopentenyl, cyclohexenyl, cycloheptenyl, and bicyclo[2.2.1]heptenyl.

[0056] As used herein, the term "fluoroalkyl" means an alkyl group as defined herein in which 1, 2, 3, 4, 5, 6, 7 or 8 hydrogen atoms are replaced by fluorine. Representative examples of fluoroalkyl include, but are not limited to, 2-fluoroethyl, 2,2,2-trifluoroethyl, trifluoromethyl, difluoromethyl, pentafluoroethyl, and trifluoropropyl, such as 3,3,3-trifluoropropyl.

[0057] The term "fluoroalkoxy" as used herein means at least one fluoroalkyl group, as defined herein, appended to the parent molecular moiety through an oxygen atom. Representative examples of fluoroalkoxy include, but are not limited to, difluoromethoxy, trifluoromethoxy and 2,2,2-trifluoroethoxy.

[0058] As used herein, the term "halogen" or "halo" means Cl, Br, I or F.

[0059] As used herein, the term "haloalkyl" means an alkyl group as defined herein wherein 1, 2, 3, 4, 5, 6, 7 or 8 of the hydrogen atoms are replaced by halogen.

[0060] The term "haloalkoxy" as used herein, means at least one haloalkyl group, as defined herein, appended to the parent molecular moiety through an oxygen atom.

[0061] As used herein, the term "halocycloalkyl" means a cycloalkyl group as defined herein wherein one or more hydrogen atoms are replaced by a halogen.

[0062] As used herein, the term "heteroalkyl" means an alkyl group as defined herein, wherein one or more carbon atoms are replaced by a heteroatom selected from S, O, P and N. Representative examples of heteroalkyl groups include, but are not limited to, alkyl ethers, secondary alkylamines, tertiary alkylamines, amides, and alkyl sulfides.

[0063] As used herein, the term "heteroaryl" refers to an aromatic monocyclic or aromatic bicyclic ring system. An aromatic monocyclic ring is a five- or six-membered ring containing at least one heteroatom independently selected from the group consisting of N, O and S (e.g., 1, 2, 3 or 4 heteroatoms independently selected from O, S and N). A five-membered aromatic monocyclic ring has two double bonds and a six-membered aromatic monocyclic ring has three double bonds. A bicyclic heteroaryl group is exemplified by a monocyclic heteroaryl ring attached to a parent molecular moiety and fused to a monocyclic cycloalkyl group as defined herein, a monocyclic aryl group as defined herein, a monocyclic heteroaryl group as defined herein, or a monocyclic heterocycle as defined herein. Representative examples of heteroaryl include, but are not limited to, indolyl, pyridinyl (including pyridin-2-yl, pyridin-3-yl, pyridin-4-yl), pyrimidinyl, pyrazinyl, pyridazinyl, pyrazolyl, pyrrolyl, benzopyrazolyl, 1,2,3-triazolyl, 1,3,4-thiadiazolyl, 1,2,4-thiadiazolyl, 1,3,4-oxadiazolyl, 1,2,4-oxadiazolyl, imidazolyl, thiazolyl, isothiazolyl, thienyl, benzimidazolyl, benzothiazolyl, benzoxazolyl, benzo oxadiazolyl, benzothiophenyl, benzofuranyl, isobenzofuranyl, furanyl, oxazolyl, isoxazolyl, purinyl, isoindolyl, quinoxalinyl, indazolyl, quinazolinyl, 1,2,4-triazinyl, 1,3,5-triazinyl, isoquinolyl, quinolyl, 6,7-dihydro-1,3-benzothiazolyl, imidazo[1,2-a]pyridinyl, naphthyridinyl, pyridoimidazolyl, thiazolo[5,4-b]pyridin-2-yl, thiazolo[5,4-d]pyrimidin-2-yl.

[0064] As used herein, the term "heterocycle" or "heterocyclic" means a monocyclic heterocycle, a bicyclic heterocycle, or a tricyclic heterocycle. A monocyclic heterocycle is a three-, four-, five-, six-, seven-, or eight-membered ring containing at least one heteroatom independently selected from the group consisting of O, N, and S. A three- or four-membered ring contains zero or one double bond and a heteroatom selected from the group consisting of O, N, and S. A five-membered ring contains zero or one double bond and one, two, or three heteroatoms selected from the group consisting of O, N, and S. A six-membered ring contains zero, one, or two double bonds and one, two, or three heteroatoms selected from the group consisting of O, N, and S. Seven- and eight-membered rings contain zero, one, two, or three double bonds and one, two, or three heteroatoms selected from the group consisting of O, N, and S. Representative examples of monocyclic heterocycles include, but are not limited to, azetidinyl, azepanyl, aziridinyl, diazepanyl, 1,3-dioxanyl, 1,3-dioxolanyl, 1,3-dithiolanyl, 1,3-dithianyl, imidazolinyl, imidazolidinyl, isothiazolinyl, isothiazolidinyl, isoxazolinyl, isoxazolidinyl, morpholinyl, oxadiazolinyl, oxadiazolidinyl, oxazolinyl, oxadiazolyl, oxazolidinyl, oxadiazol ... The term "bicyclic heterocycle" refers to a monocyclic heterocycle fused to a phenyl group, or a monocyclic heterocycle fused to a monocyclic cycloalkyl group, or a monocyclic heterocycle fused to a monocyclic cycloalkenyl group, or a monocyclic heterocycle fused to a monocyclic heterocycle, or a spiroheterocyclic group, or a bridged monocyclic heterocyclic ring system in which two non-adjacent atoms of the ring are connected by an alkylene bridge of 1, 2, 3 or 4 carbon atoms or an alkenyl bridge of 2, 3 or 4 carbon atoms. Representative examples of bicyclic heterocycles include, but are not limited to, benzopyranyl, benzothiopyranyl, chromanyl, 2,3-dihydrobenzofuranyl, 2,3-dihydrobenzothiophenyl, 2,3-dihydroisoquinoline, 2-azaspiro[3.3]heptan-2-yl, 2-oxa-6-azaspiro[3.3]heptan-6-yl, azabicyclo[2.2.1]heptyl (including 2-azabicyclo[2.2 .1]hept-2-yl), oxabicyclo[2.2.1]heptanyl (including 7-oxabicyclo[2.2.1]heptan-3-yl), azabicyclo[3.1.0]hexanyl (including 3-azabicyclo[3.1.0]hexan-3-yl), 2,3-dihydro-1H-indolyl, isoindolyl, octahydrocyclopenta[c]pyrrolyl, octahydropyrrolopyridinyl, and tetrahydroisoquinolinyl.Tricyclic heterocycles are exemplified by bicyclic heterocycles fused to a phenyl group, or bicyclic heterocycles fused to a monocyclic cycloalkyl, or bicyclic heterocycles fused to a monocyclic cycloalkenyl, or bicyclic heterocycles fused to a monocyclic heterocycle, or bicyclic heterocycles in which two non-adjacent atoms of the bicyclic ring are connected by an alkylene bridge of 1, 2, 3, or 4 carbon atoms, or an alkenylene bridge of 2, 3, or 4 carbon atoms. Examples of tricyclic heterocycles include, but are not limited to, octahydro-2,5-epoxypentalene, hexahydro-2H-2,5-methanocyclopenta[b]furan, hexahydro-1H-1,4-methanocyclopenta[c]furan, aza-adamantane (1-azatricyclo[3.3.1.13,7]decane), and oxa-adamantane (2-oxatricyclo[3.3.1.13,7]decane). The monocyclic heterocycle, bicyclic heterocycle, and tricyclic heterocycle are attached to the parent molecular moiety through any carbon atom or any nitrogen atom contained within these rings, and can be unsubstituted or substituted.

[0065] As used herein, the term "hydroxyl" or "hydroxy" refers to an -OH group.

[0066] The term "hydroxyalkyl" as used herein, means at least one -OH group attached to the parent molecular moiety through an alkylene group, as defined herein.

[0067] The term "hydroxyfluoroalkyl" as used herein, means at least one -OH group, attached to the parent molecular moiety through a fluoroalkyl group, as defined herein.

[0068] In some cases, the number of carbon atoms in a hydrocarbyl substituent (e.g., alkyl or cycloalkyl) is indicated by the prefix "C x -C y -" indicates where x is the minimum number of carbon atoms in the substituent and y is the maximum number. Thus, for example, "C1-C3-alkyl" refers to an alkyl substituent containing from 1 to 3 carbon atoms.

[0069] The term "substituent" refers to a group (other than hydrogen) "substituted" on a group such as an alkyl, alkenyl, alkynyl, aryl, heteroaryl, cycloalkyl, heteroalkyl or heterocyclic group at any atom of the group. Any atom may be substituted.

[0070] The term "substituted" refers to a group that can be further substituted by one or more non-hydrogen substituent groups. Substituent groups include but are not limited to halogen, =O (oxo), =S (thio), cyano, nitro, fluoroalkyl, alkoxyfluoroalkyl, fluoroalkoxy, alkyl, alkenyl, alkynyl, haloalkyl, haloalkoxy, heteroalkyl, cycloalkyl, cycloalkenyl, aryl, heteroaryl, heterocycle, cycloalkylalkyl, heteroarylalkyl, arylalkyl, hydroxyl, hydroxyalkyl, alkoxy, alkoxyalkyl, alkylidene, aryloxy, phenoxy, benzyloxy, amino, alkylamino, acylamino, aminoalkyl, arylamino, sulfonylamino, sulfinylamino, sulfonyl, alkylsulfonyl, arylsulfonyl, aminosulfonyl, sulfinyl,-COOH, ketone, amide, carbamate and acyl. In certain embodiments, the group is optionally substituted. In certain embodiments, the group is optionally substituted by 1,2,3,4 or 5 substituents. In some embodiments, aryl, heteroaryl, cycloalkyl or heterocycle is optionally substituted with 1, 2, 3, 4 or 5 substituents. In some embodiments, aryl, heteroaryl, cycloalkyl or heterocycle can be independently unsubstituted or substituted with 1, 2 or 3 substituents.

[0071] For the compounds described herein, radicals and substituents may be chosen based on the valences allowed by the atoms and substituents such that selection and substitution results in a stable compound, e.g., one that does not spontaneously transform (e.g., by rearrangement, cyclization, elimination, etc.).

[0072] As used herein, the term "allosteric site" refers to a ligand binding site that is topologically different from the orthosteric binding site.

[0073] As used herein, the term "modulator" refers to a molecular entity (such as, but not limited to, ligands and disclosed compounds) that modulates the activity of a targeted receptor protein.

[0074] As used herein, the term "ligand" refers to a natural or synthetic molecular entity that is capable of associating or binding to a receptor to form a complex and mediate, prevent or alter a biological effect. Thus, the term "ligand" includes allosteric modulators, inhibitors, activators, agonists, antagonists, natural substrates, and analogs of natural substrates.

[0075] As used herein, the terms "natural ligand" and "endogenous ligand" are used interchangeably and refer to a naturally occurring ligand that is found in nature that binds to a receptor.

[0076] As used herein, the term "orthosteric site" refers to the primary binding site on a receptor that is recognized by the endogenous ligand or agonist of the receptor. For example, the orthosteric site in the mAChR M4 receptor is the site where acetylcholine binds.

[0077] As used herein, the term "mAChR M4 receptor positive allosteric modulator" refers to any exogenously administered compound or agent that directly or indirectly enhances the activity of the mAChR M4 receptor of an animal (particularly a mammal, such as a human) in the presence or absence of acetylcholine or another agonist. For example, in the presence of extracellular acetylcholine, a mAChR M4 receptor positive allosteric modulator can increase the activity of the mAChR M4 receptor in a cell. The cell can be a Chinese hamster ovary (CHO-K1) cell transfected with human mAChRM4. The cell can be a Chinese hamster ovary (CHO-K1) cell transfected with a rat mAChR M4 receptor. The cell can be a Chinese hamster ovary (CHO-K1) cell transfected with a mammalian mAChR M4. The term "mAChR M4 receptor positive allosteric modulator" includes compounds that are "mAChR M4 receptor allosteric potentiators" or "mAChR M4 receptor allosteric agonists", as well as compounds with mixed activity, which include the pharmacology of "mAChR M4 receptor allosteric potentiators" and "mAChR M4 receptor allosteric agonists". The term "mAChR M4 receptor positive allosteric modulator" also includes compounds that are "mAChRM4 receptor allosteric enhancers".

[0078] As used herein, the term "mAChR M4 receptor allosteric potentiator" refers to any exogenously administered compound or agent that directly or indirectly enhances the response produced by an endogenous ligand (such as acetylcholine) when the endogenous ligand binds to the orthosteric site of the mAChR M4 receptor of an animal (particularly a mammal, such as a human). The mAChR M4 receptor allosteric potentiator binds to a site other than the orthosteric site, i.e., the allosteric site, and positively enhances the response of the receptor to an agonist or endogenous ligand. In some embodiments, the allosteric potentiator does not induce receptor desensitization, and the activity of the compound as a mAChR M4 receptor allosteric potentiator can provide advantages over the use of pure mAChR M4 receptor orthosteric agonists. These advantages can include, for example, increased safety margins, higher tolerability, reduced potential for abuse, and reduced toxicity.

[0079] As used herein, the term "mAChR M4 receptor allosteric enhancer" refers to any exogenously administered compound or agent that directly or indirectly enhances the response produced by an endogenous ligand (such as acetylcholine) in an animal (particularly a mammal, such as a human). In some embodiments, the allosteric enhancer increases the affinity of a natural ligand or agonist for the orthosteric site. In some embodiments, the allosteric enhancer increases the efficacy of an agonist. The mAChR M4 receptor allosteric enhancer binds to a site other than the orthosteric site, i.e., the allosteric site, and positively enhances the response of the receptor to an agonist or endogenous ligand. The allosteric enhancer itself has no effect on the receptor and requires the presence of an agonist or natural ligand to achieve receptor action.

[0080] As used herein, the term "mAChR M4 receptor allosteric agonist" refers to any exogenously administered compound or agent that directly activates the activity of the mAChR M4 receptor in the absence of an endogenous ligand (such as acetylcholine) in an animal (particularly a mammal, such as a human). The mAChR M4 receptor allosteric agonist binds to a site that is different from the orthosteric acetylcholine site of the mAChR M4 receptor. Because the presence of an endogenous ligand is not required, if the cholinergic tone of a given synapse is low, the activity of the compound as a mAChR M4 receptor allosteric agonist provides an advantage.

[0081] As used herein, the term "neutral allosteric ligand of mAChR M4 receptor" refers to any exogenously administered compound or agent that binds to the allosteric site without affecting the binding or function of the agonist or natural ligand at the orthosteric site in animals (particularly mammals, such as humans). However, neutral allosteric ligands can block the effects of other allosteric modulators that act through the same site.

[0082] For the recitation of numerical ranges herein, each intervening number with equal precision therebetween is expressly contemplated. For example, for the range 6-9, the numbers 7 and 8 are contemplated in addition to 6 and 9, and for the range 6.0-7.0, the numbers 6.0, 6.1, 6.2, 6.3, 6.4, 6.5, 6.6, 6.7, 6.8, 6.9, and 7.0 are expressly contemplated.

[0083] 2. Embodiments of the present invention

[0084] Embodiments of the present invention are disclosed below. The first embodiment is denoted as E1, the second embodiment is denoted as E2, and so on.

[0085] E1. A compound having formula (I),

[0086]

[0087] or a pharmaceutically acceptable salt thereof, wherein:

[0088] X is CR 5a or N;

[0089] R 1 is selected from hydrogen, C1-C4-alkyl, C1-C4-haloalkyl, C1-C4-hydroxyalkyl, C3-C6-cycloalkyl, -L 1 -C3-C6-cycloalkyl, halogen, -L 1 OR a OR a ;

[0090] R 2 is selected from hydrogen, C1-C4-alkyl, C1-C4-haloalkyl, C3-C6-cycloalkyl, halogen, -L 2 OR b , OR b 、NHR b and N(R b )2;

[0091] R 3 is selected from hydrogen, C1-C4-alkyl, C1-C4-haloalkyl, C3-C6-cycloalkyl, halogen, -L 3 OR c OR c ;

[0092] Each R 4 Independently selected from C1-C4-alkyl, OR d , -L 4 OR d and halogens;

[0093] Each R 5 independently selected from C1-C4-alkyl, C1-C4-haloalkyl, OR e , -L 5 OR e , 5-membered or 6-membered heteroaryl, phenyl, C3-C6-cycloalkyl and halogen; or

[0094] Two of the R 5 Together with the carbon atoms to which they are attached, they form a 5- to 8-membered fused monocyclic heterocyclic ring containing 1-2 heteroatoms independently selected from O, N and S, the fused monocyclic heterocyclic ring being optionally substituted by 1-4 R 6 replace;

[0095] R 5a is selected from hydrogen, C1-C4-alkyl, C1-C4-haloalkyl, C3-C6-cycloalkyl, OR e , -L 5 OR e and halogens;

[0096] Each R 5b independently selected from C1-C2-alkyl (e.g., methyl), C1-C2-fluoroalkyl (e.g., trifluoromethyl, difluoromethyl), C3-C4-cycloalkyl (e.g., cyclopropyl), -OC1-C2-alkyl (e.g., methoxy), -OC1-C2-fluoroalkyl (e.g., trifluoromethoxy, difluoromethoxy), cyano and halogen (e.g., fluorine, chlorine);

[0097] Each R 6 independently selected from oxo, C1-C4-alkyl, OR f , -L 6 OR f and halogens;

[0098] R 7 is selected from hydrogen, C1-C4-alkyl, C1-C4-haloalkyl, C3-C6-cycloalkyl, halogen, -L 2 OR b , OR b 、NHR b and N(R b )2;

[0099] o is 0, 1, or 2;

[0100] m is 0, 1, or 2;

[0101] n is 0, 1, or 2;

[0102] L 1 , L 2 , L 3 , L 4 , L 5 and L 6 is independently at each occurrence C1-C3-alkylene; and

[0103] R a , R b , R c , R d , R e and R f are independently selected at each occurrence from C1-C4-alkyl, C1-C4-haloalkyl and C3-C6-cycloalkyl, wherein both R b Together with the nitrogen atom to which they are attached they optionally form a 4- to 7-membered monocyclic heterocyclyl group which is optionally substituted with 1-4 substituents independently selected from halogen, C1-C4-alkyl and C1-C4-haloalkyl.

[0104] E2. The compound as described in Example E1, which has formula (IA),

[0105]

[0106] or a pharmaceutically acceptable salt thereof, wherein

[0107] Each R 5c are independently selected from hydrogen, C1-C4-alkyl, C1-C4-haloalkyl, OR e , -L 5 OR e , 5-membered or 6-membered heteroaryl, phenyl, C3-C6-cycloalkyl and halogen; or

[0108] Two of the R 5c Together with the carbon atoms to which they are attached, they form a 5- to 8-membered fused monocyclic heterocyclic ring containing 1-2 heteroatoms independently selected from O, N and S, the fused monocyclic heterocyclic ring being optionally substituted by 1-4 R 6 replace.

[0109] E3. A compound as described in Example E1 or E2, or a pharmaceutically acceptable salt thereof, wherein two R 5 or R 5c Together with the carbon atoms to which they are attached they form a 5- to 8-membered fused monocyclic heterocyclic ring.

[0110] E4. A compound as described in any one of Embodiments E1 to E3, or a pharmaceutically acceptable salt thereof, wherein R 5 or R 5c The formed 5- to 8-membered fused monocyclic heterocyclic ring contains 1-2 heteroatoms independently selected from O and N, and is optionally substituted by 1-2 R 6 replace.

[0111] E5. A compound of formula (IB) as described in any one of Embodiments E1 to E3,

[0112]

[0113] or a pharmaceutically acceptable salt thereof, wherein

[0114] G 1 yes

[0115] and

[0116] Each q is independently 0, 1, 2, 3 or 4.

[0117] E6. A compound as described in Example E5, or a pharmaceutically acceptable salt thereof, wherein

[0118] G 1 yes

[0119] and

[0120] R 5a is hydrogen, C1-C4-alkyl or halogen.

[0121] E7. A compound as described in Example E6, or a pharmaceutically acceptable salt thereof, wherein

[0122] G 1 yes

[0123]

[0124] E8. A compound of formula (Ia) as described in any one of Embodiments E1 to E5

[0125]

[0126] or a pharmaceutically acceptable salt thereof.

[0127] E9. A compound of formula (Ib) as described in any one of Embodiments E1 to E5

[0128]

[0129] or a pharmaceutically acceptable salt thereof.

[0130] E10. The compound of any one of Embodiments E1 to E9, or a pharmaceutically acceptable salt thereof, wherein n is 0.

[0131] E11. The compound as described in Embodiment E1 or E2, or a pharmaceutically acceptable salt thereof, wherein each R 5 or R 5c are independently selected from hydrogen, C1-C4-alkyl, C1-C4-haloalkyl, OR e , -L 5 OR e , 5-membered or 6-membered heteroaryl, phenyl, C3-C6-cycloalkyl and halogen.

[0132] E12. The compound of Embodiment E1, E2 or E11, or a pharmaceutically acceptable salt thereof, wherein each R 5 or R 5c are independently selected from hydrogen, C1-C4-alkyl, C1-C4-haloalkyl, OR e , -L 5 OR e and halogens.

[0133] E13. The compound of formula (IB) as described in Example E12

[0134]

[0135] or a pharmaceutically acceptable salt thereof, wherein

[0136] G 1 yes

[0137]

[0138] E14. The compound as described in Example E13, or a pharmaceutically acceptable salt thereof, wherein

[0139] G 1 yes

[0140]

[0141] E15. The compound of embodiment E14, or a pharmaceutically acceptable salt thereof, wherein

[0142] G 1 yes

[0143]

[0144] E16. A compound as described in any one of Embodiments E1 to E15, or a pharmaceutically acceptable salt thereof, wherein R 3 is hydrogen, C1-C4-alkyl, C3-C6-cycloalkyl or -L 3 OR c .

[0145] E17. The compound of embodiment E16, or a pharmaceutically acceptable salt thereof, wherein R 3 It is C1-C4-alkyl.

[0146] E18. The compound of embodiment E17, or a pharmaceutically acceptable salt thereof, wherein R 3 It's methyl.

[0147] E19. A compound as described in any one of Embodiments E1 to E18, or a pharmaceutically acceptable salt thereof, wherein R 1 is hydrogen, C1-C4-alkyl, C1-C4-haloalkyl, C3-C6-cycloalkyl, -L 1 -C3-C6-cycloalkyl or -L 1 OR a .

[0148] E20. The compound of embodiment E19, or a pharmaceutically acceptable salt thereof, wherein R 1 It is C1-C4-alkyl.

[0149] E21. The compound of embodiment E19, or a pharmaceutically acceptable salt thereof, wherein R 1 It's hydrogen.

[0150] E22. The compound of embodiment E19, or a pharmaceutically acceptable salt thereof, wherein R 1 It is a C1-C4-fluoroalkyl group.

[0151] E23. The compound of embodiment E22, or a pharmaceutically acceptable salt thereof, wherein R 1 It is trifluoromethyl.

[0152] E24. The compound of embodiment E22, or a pharmaceutically acceptable salt thereof, wherein R 1 It is difluoromethyl.

[0153] E25. A compound as described in any one of Embodiments E1 to E24, or a pharmaceutically acceptable salt thereof, wherein R 2 is selected from hydrogen, C1-C4-alkyl, C3-C6-cycloalkyl, -L 2 OR b , OR b 、NHR b and N(R b )2.

[0154] E26. The compound of embodiment E25, or a pharmaceutically acceptable salt thereof, wherein R 2 It is C1-C4-alkyl.

[0155] E27. The compound of embodiment E25, or a pharmaceutically acceptable salt thereof, wherein R 2 It's hydrogen.

[0156] E28. The compound of embodiment E25, or a pharmaceutically acceptable salt thereof, wherein R 2 It's methyl.

[0157] E29. A compound as described in any one of Embodiments E1 to E28, or a pharmaceutically acceptable salt thereof, wherein R 4 is C1-C4-alkyl or halogen.

[0158] E30. A compound as described in any one of embodiments E1 to E29, or a pharmaceutically acceptable salt thereof, wherein o is 0.

[0159] E31. A compound as described in any one of Embodiments E1 to E29, or a pharmaceutically acceptable salt thereof, wherein o is 1.

[0160] E32. A compound as described in any one of Embodiments E1 to E31, or a pharmaceutically acceptable salt thereof, wherein R 7 is selected from hydrogen, C1-C4-alkyl, C3-C6-cycloalkyl, halogen and N(R b )2.

[0161] E33. The compound of embodiment E32, or a pharmaceutically acceptable salt thereof, wherein R 7 is selected from the group consisting of hydrogen, methyl, cyclopropyl, fluoro, chloro and azetidin-1-yl.

[0162] E34. The compound of embodiment E33, or a pharmaceutically acceptable salt thereof, wherein R 7 It's hydrogen.

[0163] E35. A compound as described in any one of embodiments E1 to E5 or E8 to E34, or a pharmaceutically acceptable salt thereof, wherein X is N.

[0164] E36. A compound as described in any one of embodiments E1 to E34, or a pharmaceutically acceptable salt thereof, wherein X is CR 5a .

[0165] E37. The compound of embodiment E36, or a pharmaceutically acceptable salt thereof, wherein R 5a It's hydrogen.

[0166] E38. A compound as described in embodiment E1, which is selected from the group consisting of:

[0167] 7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)piperidin-1-yl)-8-methyl-4H-pyrimido[1,2-b]pyridazin-4-one;

[0168] 7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)piperidin-1-yl)-9-methyl-4H-pyrimido[1,2-b]pyridazin-4-one;

[0169] 3-Chloro-7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)piperidin-1-yl)-8-methyl-4H-pyrimido[1,2-b]pyridazin-4-one;

[0170] 8-methyl-7-(4-((6-methylpyridin-3-yl)oxy)piperidin-1-yl)-4H-pyrimido[1,2-b]pyridazin-4-one;

[0171] 7-(4-(4-fluorophenoxy)piperidin-1-yl)-8-methyl-4H-pyrimido[1,2-b]pyridazin-4-one;

[0172] 7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl)oxy)piperidin-1-yl)-8-methyl-4H-pyrimido[1,2-b]pyridazin-4-one;

[0173] 3-Chloro-8-methyl-7-(4-((8-methyl-2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)piperidin-1-yl)-4H-pyrimido[1,2-b]pyridazin-4-one;

[0174] 8-methyl-7-(4-((8-methyl-2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)piperidin-1-yl)-4H-pyrimido[1,2-b]pyridazin-4-one;

[0175] 7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)piperidin-1-yl)-3-fluoro-2,8-dimethyl-4H-pyrimido[1,2-b]pyridazin-4-one;

[0176] 7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)piperidin-1-yl)-3,8-dimethyl-4H-pyrimido[1,2-b]pyridazin-4-one;

[0177] 3-cyclopropyl-7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)piperidin-1-yl)-8-methyl-4H-pyrimido[1,2-b]pyridazin-4-one;

[0178] 3-Chloro-7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl)oxy)piperidin-1-yl)-8-methyl-4H-pyrimido[1,2-b]pyridazin-4-one;

[0179] 7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)piperidin-1-yl)-2,8-dimethyl-4H-pyrimido[1,2-b]pyridazin-4-one;

[0180] 7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)piperidin-1-yl)-8-methyl-2-(trifluoromethyl)-4H-pyrimido[1,2-b]pyridazin-4-one;

[0181] 7-((3R,4R)-4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl)oxy)-3-fluoropiperidin-1-yl)-8-methyl-4H-pyrimido[1,2-b]pyridazin-4-one;

[0182] 7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)piperidin-1-yl)-3-fluoro-2,8,9-trimethyl-4H-pyrimido[1,2-b]pyridazin-4-one;

[0183] 7-((3S,4R)-4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl)oxy)-3-fluoropiperidin-1-yl)-2,8-dimethyl-4H-pyrimido[1,2-b]pyridazin-4-one;

[0184] 7-((3S,4R)-4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl)oxy)-3-fluoropiperidin-1-yl)-8-methyl-4H-pyrimido[1,2-b]pyridazin-4-one;

[0185] 3-(azetidin-1-yl)-7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)piperidin-1-yl)-8-methyl-4H-pyrimido[1,2-b]pyridazin-4-one;

[0186] 7-((3R,4R)-4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl)oxy)-3-fluoropiperidin-1-yl)-3-fluoro-2,8-dimethyl-4H-pyrimido[1,2-b]pyridazin-4-one;

[0187] 7-((3S,4S)-4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl)oxy)-3-fluoropiperidin-1-yl)-3-fluoro-2,8-dimethyl-4H-pyrimido[1,2-b]pyridazin-4-one;

[0188] 7-((3S,4S)-4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)-3-fluoropiperidin-1-yl)-3-fluoro-2,8-dimethyl-4H-pyrimido[1,2-b]pyridazin-4-one;

[0189] 7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)piperidin-1-yl-4-d)-8-methyl-4H-pyrimido[1,2-b]pyridazin-4-one;

[0190] 7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)piperidin-1-yl-4-d)-2,8-dimethyl-4H-pyrimido[1,2-b]pyridazin-4-one;

[0191] 7-((3R,4R)-4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)-3-fluoropiperidin-1-yl)-8-methyl-4H-pyrimido[1,2-b]pyridazin-4-one;

[0192] 7-((3S,4S)-4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)-3-fluoropiperidin-1-yl)-8-methyl-4H-pyrimido[1,2-b]pyridazin-4-one;

[0193] 7-((3R,4S)-4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl)oxy)-3-fluoropiperidin-1-yl)-8-methyl-4H-pyrimido[1,2-b]pyridazin-4-one;

[0194] 7-((3R,4S)-4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl)oxy)-3-fluoropiperidin-1-yl)-2,8-dimethyl-4H-pyrimido[1,2-b]pyridazin-4-one;

[0195] 7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)piperidin-1-yl)-2,3,8-trimethyl-4H-pyrimido[1,2-b]pyridazin-4-one;

[0196] 2-(Difluoromethyl)-7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)piperidin-1-yl)-8-methyl-4H-pyrimido[1,2-b]pyridazin-4-one;

[0197] 2-Cyclopropyl-7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)piperidin-1-yl)-8-methyl-4H-pyrimido[1,2-b]pyridazin-4-one;

[0198] 7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)piperidin-1-yl-4-d)-8-methyl-2-(trifluoromethyl)-4H-pyrimido[1,2-b]pyridazin-4-one;

[0199] 7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)piperidin-1-yl-4-d)-3-fluoro-2,8-dimethyl-4H-pyrimido[1,2-b]pyridazin-4-one;

[0200] 7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)piperidin-1-yl-4-d)-2,3,8-trimethyl-4H-pyrimido[1,2-b]pyridazin-4-one;

[0201] 3-Chloro-7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)piperidin-1-yl)-2,8-dimethyl-4H-pyrimido[1,2-b]pyridazin-4-one;

[0202] 7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl)oxy)piperidin-1-yl-4-d)-8-methyl-4H-pyrimido[1,2-b]pyridazin-4-one;

[0203] 7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl)oxy)piperidin-1-yl-4-d)-8-methyl-2-(trifluoromethyl)-4H-pyrimido[1,2-b]pyridazin-4-one;

[0204] 7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl)oxy)piperidin-1-yl-4-d)-3-fluoro-2,8-dimethyl-4H-pyrimido[1,2-b]pyridazin-4-one;

[0205] 7-((3R,4R)-4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)-3-fluoropiperidin-1-yl)-2,8-dimethyl-4H-pyrimido[1,2-b]pyridazin-4-one;

[0206] 2,8-Dimethyl-7-(4-((6-methylpyridin-3-yl)oxy)piperidin-1-yl)-4H-pyrimido[1,2-b]pyridazin-4-one;

[0207] 7-((3R,4R)-4-(Benzo[d][1,3]dioxol-5-yloxy)-3-fluoropiperidin-1-yl)-8-methyl-4H-pyrimido[1,2-b]pyridazin-4-one;

[0208] 7-((3R,4R)-3-fluoro-4-(isochroman-6-yloxy)piperidin-1-yl)-8-methyl-4H-pyrimido[1,2-b]pyridazin-4-one;

[0209] 7-((3R,4R)-4-((3,4-dihydro-2H-benzo[b][1,4]dioxepin-7-yl)oxy)-3-fluoropiperidin-1-yl)-8-methyl-4H-pyrimido[1,2-b]pyridazin-4-one;

[0210] 7-((3R,4R)-4-((2,3-dihydrobenzofuran-5-yl)oxy)-3-fluoropiperidin-1-yl)-8-methyl-4H-pyrimido[1,2-b]pyridazin-4-one;

[0211] 7-((3R,4R)-4-(chroman-7-yloxy)-3-fluoropiperidin-1-yl)-8-methyl-4H-pyrimido[1,2-b]pyridazin-4-one;

[0212] 7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)piperidin-1-yl)-2,8,9-trimethyl-4H-pyrimido[1,2-b]pyridazin-4-one;

[0213] 7-(4-(Benzo[d][1,3]dioxol-5-yloxy)piperidin-1-yl-4-d)-2,8-dimethyl-4H-pyrimido[1,2-b]pyridazin-4-one;

[0214] 7-(4-((3,4-dihydro-2H-benzo[b][1,4]dioxepin-7-yl)oxy)piperidin-1-yl-4-d)-2,8-dimethyl-4H-pyrimido[1,2-b]pyridazin-4-one;

[0215] 7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl)oxy)piperidin-1-yl-4-d)-2,3,8-trimethyl-4H-pyrimido[1,2-b]pyridazin-4-one;

[0216] 2-(Difluoromethyl)-7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl)oxy)piperidin-1-yl-4-d)-8-methyl-4H-pyrimido[1,2-b]pyridazin-4-one;

[0217] 7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl)oxy)piperidin-1-yl-4-d)-2,8-dimethyl-4H-pyrimido[1,2-b]pyridazin-4-one;

[0218] 7-(4-(Benzo[d][1,3]dioxol-5-yloxy)piperidin-1-yl-4-d)-8-methyl-2-(trifluoromethyl)-4H-pyrimido[1,2-b]pyridazin-4-one;

[0219] 7-(4-((3,4-dihydro-2H-benzo[b][1,4]dioxepin-7-yl)oxy)piperidin-1-yl-4-d)-8-methyl-2-(trifluoromethyl)-4H-pyrimido[1,2-b]pyridazin-4-one;

[0220] 2-(Difluoromethyl)-7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)piperidin-1-yl-4-d)-8-methyl-4H-pyrimido[1,2-b]pyridazin-4-one;

[0221] 7-(4-(Benzo[d][1,3]dioxol-5-yloxy)piperidin-1-yl-4-d)-2-(difluoromethyl)-8-methyl-4H-pyrimido[1,2-b]pyridazin-4-one;

[0222] 2-(Difluoromethyl)-7-(4-((3,4-dihydro-2H-benzo[b][1,4]dioxepin-7-yl)oxy)piperidin-1-yl-4-d)-8-methyl-4H-pyrimido[1,2-b]pyridazin-4-one;

[0223] 7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)piperidin-1-yl)-8,9-dimethyl-2-(trifluoromethyl)-4H-pyrimido[1,2-b]pyridazin-4-one;

[0224] 2-(Difluoromethyl)-7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)piperidin-1-yl)-8,9-dimethyl-4H-pyrimido[1,2-b]pyridazin-4-one;

[0225] 7-(4-(Benzo[d][1,3]dioxol-5-yloxy)piperidin-1-yl-4-d)-8-methyl-4H-pyrimido[1,2-b]pyridazin-4-one;

[0226] 7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)piperidin-1-yl-4-d)-8,9-dimethyl-4H-pyrimido[1,2-b]pyridazin-4-one;

[0227] 7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl)oxy)piperidin-1-yl-4-d)-8,9-dimethyl-4H-pyrimido[1,2-b]pyridazin-4-one;

[0228] 7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl)oxy)piperidin-1-yl)-8,9-dimethyl-4H-pyrimido[1,2-b]pyridazin-4-one;

[0229] 7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)piperidin-1-yl)-8,9-dimethyl-4H-pyrimido[1,2-b]pyridazin-4-one;

[0230] 2,8-Dimethyl-7-(4-((1-oxo-1,2,3,4-tetrahydroisoquinolin-7-yl)oxy)piperidin-1-yl)-4H-pyrimido[1,2-b]pyridazin-4-one;

[0231] 2,8-Dimethyl-7-(4-((2-methyl-1-oxo-1,2,3,4-tetrahydroisoquinolin-7-yl)oxy)piperidin-1-yl)-4H-pyrimido[1,2-b]pyridazin-4-one;

[0232] 2,8-Dimethyl-7-(4-((2-methyl-1-oxo-1,2,3,4-tetrahydroisoquinolin-6-yl)oxy)piperidin-1-yl)-4H-pyrimido[1,2-b]pyridazin-4-one;

[0233] 7-(4-((6-methoxy-5-methylpyridin-3-yl)oxy)piperidin-1-yl)-8-methyl-4H-pyrimido[1,2-b]pyridazin-4-one;

[0234] 7-(4-((6-(methoxymethyl)pyridin-3-yl)oxy)piperidin-1-yl)-8-methyl-4H-pyrimido[1,2-b]pyridazin-4-one;

[0235] 8,9-dimethyl-7-(4-(p-tolyloxy)piperidin-1-yl)-4H-pyrimido[1,2-b]pyridazin-4-one;

[0236] 2-(Difluoromethyl)-8,9-dimethyl-7-(4-((6-methylpyridin-3-yl)oxy)piperidin-1-yl)-4H-pyrimido[1,2-b]pyridazin-4-one;

[0237] 7-(4-(4-chlorophenoxy)piperidin-1-yl)-2-(difluoromethyl)-8,9-dimethyl-4H-pyrimido[1,2-b]pyridazin-4-one;

[0238] 8,9-dimethyl-7-(4-(4-(trifluoromethyl)phenoxy)piperidin-1-yl)-4H-pyrimido[1,2-b]pyridazin-4-one;

[0239] 2-(Difluoromethyl)-8,9-dimethyl-7-(4-(4-(trifluoromethyl)phenoxy)piperidin-1-yl)-4H-pyrimido[1,2-b]pyridazin-4-one;

[0240] 2,8-Dimethyl-7-(4-((1-oxo-1,2,3,4-tetrahydroisoquinolin-6-yl)oxy)piperidin-1-yl)-4H-pyrimido[1,2-b]pyridazin-4-one;

[0241] 7-(4-(4-fluorophenoxy)piperidin-1-yl)-8,9-dimethyl-4H-pyrimido[1,2-b]pyridazin-4-one;

[0242] 2-(Difluoromethyl)-7-(4-(4-fluorophenoxy)piperidin-1-yl)-8,9-dimethyl-4H-pyrimido[1,2-b]pyridazin-4-one;

[0243] 2-(Difluoromethyl)-8,9-dimethyl-7-(4-(p-tolyloxy)piperidin-1-yl)-4H-pyrimido[1,2-b]pyridazin-4-one;

[0244] 8,9-dimethyl-7-(4-((6-methylpyridin-3-yl)oxy)piperidin-1-yl)-4H-pyrimido[1,2-b]pyridazin-4-one;

[0245] 8,9-dimethyl-7-(4-((6-methylpyridin-3-yl)oxy)piperidin-1-yl)-4H-pyrimido[1,2-b]pyridazin-4-one;

[0246] 7-(4-(3-fluoro-4-methylphenoxy)piperidin-1-yl)-8-methyl-4H-pyrimido[1,2-b]pyridazin-4-one;

[0247] 7-(4-(4-ethylphenoxy)piperidin-1-yl)-8-methyl-4H-pyrimido[1,2-b]pyridazin-4-one;

[0248] 7-(4-(4-isopropylphenoxy)piperidin-1-yl)-8-methyl-4H-pyrimido[1,2-b]pyridazin-4-one;

[0249] 8-methyl-7-(4-(4-propylphenoxy)piperidin-1-yl)-4H-pyrimido[1,2-b]pyridazin-4-one;

[0250] 8-methyl-7-(4-(p-tolyloxy)piperidin-1-yl-4-d)-4H-pyrimido[1,2-b]pyridazin-4-one;

[0251] 7-(4-((6-(methoxymethyl)-5-methylpyridin-3-yl)oxy)piperidin-1-yl)-8-methyl-4H-pyrimido[1,2-b]pyridazin-4-one;

[0252] 7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl)oxy)piperidin-1-yl)-2-ethyl-8,9-dimethyl-4H-pyrimido[1,2-b]pyridazin-4-one;

[0253] 7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl)oxy)piperidin-1-yl)-2-isopropyl-8,9-dimethyl-4H-pyrimido[1,2-b]pyridazin-4-one;

[0254] 7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl)oxy)piperidin-1-yl)-2-(methoxymethyl)-8,9-dimethyl-4H-pyrimido[1,2-b]pyridazin-4-one;

[0255] 8-cyclopropyl-7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl)oxy)piperidin-1-yl)-9-methyl-4H-pyrimido[1,2-b]pyridazin-4-one;

[0256] 9-cyclopropyl-7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl)oxy)piperidin-1-yl)-8-methyl-4H-pyrimido[1,2-b]pyridazin-4-one;

[0257] 7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)piperidin-1-yl-4-d)-2,8,9-trimethyl-4H-pyrimido[1,2-b]pyridazin-4-one;

[0258] 7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)piperidin-1-yl-4-d)-8,9-dimethyl-2-(trifluoromethyl)-4H-pyrimido[1,2-b]pyridazin-4-one;

[0259] 7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)piperidin-1-yl-4-d)-2-isopropyl-8,9-dimethyl-4H-pyrimido[1,2-b]pyridazin-4-one;

[0260] 9-cyclopropyl-7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)piperidin-1-yl-4-d)-8-methyl-4H-pyrimido[1,2-b]pyridazin-4-one;

[0261] 7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)piperidin-1-yl-4-d)-2-ethyl-8,9-dimethyl-4H-pyrimido[1,2-b]pyridazin-4-one;

[0262] 7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)piperidin-1-yl)-2-isopropyl-8,9-dimethyl-4H-pyrimido[1,2-b]pyridazin-4-one;

[0263] 7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)piperidin-1-yl)-2-ethyl-8,9-dimethyl-4H-pyrimido[1,2-b]pyridazin-4-one;

[0264] 2-(Cyclopentylmethyl)-7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)piperidin-1-yl-4-d)-8,9-dimethyl-4H-pyrimido[1,2-b]pyridazin-4-one;

[0265] 7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)piperidin-1-yl-4-d)-8-(methoxymethyl)-9-methyl-4H-pyrimido[1,2-b]pyridazin-4-one;

[0266] 7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)piperidin-1-yl)-8-(methoxymethyl)-9-methyl-4H-pyrimido[1,2-b]pyridazin-4-one;

[0267] 7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)piperidin-1-yl-4-d)-9-(methoxymethyl)-8-methyl-4H-pyrimido[1,2-b]pyridazin-4-one;

[0268] 7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)piperidin-1-yl)-9-(methoxymethyl)-8-methyl-4H-pyrimido[1,2-b]pyridazin-4-one;

[0269] 8-cyclopropyl-7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)piperidin-1-yl-4-d)-9-methyl-4H-pyrimido[1,2-b]pyridazin-4-one;

[0270] 8-cyclopropyl-7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)piperidin-1-yl)-9-methyl-4H-pyrimido[1,2-b]pyridazin-4-one;

[0271] 7-((2R,4R)-4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl)oxy)-2-methylpiperidin-1-yl)-8-methyl-4H-pyrimido[1,2-b]pyridazin-4-one;

[0272] 7-((2S,4R)-4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl)oxy)-2-methylpiperidin-1-yl)-8-methyl-4H-pyrimido[1,2-b]pyridazin-4-one;

[0273] 7-((2R,4S)-4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl)oxy)-2-methylpiperidin-1-yl)-8-methyl-4H-pyrimido[1,2-b]pyridazin-4-one;

[0274] 7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)piperidin-1-yl-4-d)-2-(methoxymethyl)-8-methyl-4H-pyrimido[1,2-b]pyridazin-4-one;

[0275] 7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)piperidin-1-yl)-2-(methoxymethyl)-8-methyl-4H-pyrimido[1,2-b]pyridazin-4-one;

[0276] 7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)piperidin-1-yl-4-d)-2-(methoxymethyl)-8,9-dimethyl-4H-pyrimido[1,2-b]pyridazin-4-one;

[0277] 7-((3S,4R)-4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)-3-fluoropiperidin-1-yl)-8-methyl-4H-pyrimido[1,2-b]pyridazin-4-one;

[0278] 7-((3R,4S)-4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)-3-fluoropiperidin-1-yl)-8-methyl-4H-pyrimido[1,2-b]pyridazin-4-one;

[0279] 7-((3R,4S)-4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl)oxy)-3-methylpiperidin-1-yl)-8-methyl-4H-pyrimido[1,2-b]pyridazin-4-one;

[0280] 7-((3R,4R)-4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl)oxy)-3-methylpiperidin-1-yl)-8-methyl-4H-pyrimido[1,2-b]pyridazin-4-one;

[0281] 7-((3R,4R)-4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl)oxy)-3-methylpiperidin-1-yl)-8-methyl-4H-pyrimido[1,2-b]pyridazin-4-one;

[0282] 7-((2R,4R)-4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl)oxy)-2-methylpiperidin-1-yl)-8-methyl-4H-pyrimido[1,2-b]pyridazin-4-one;

[0283] 7-((3S,4S)-4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)-3-fluoropiperidin-1-yl)-8,9-dimethyl-4H-pyrimido[1,2-b]pyridazin-4-one;

[0284] 7-((3S,4R)-4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)-3-fluoropiperidin-1-yl)-8,9-dimethyl-4H-pyrimido[1,2-b]pyridazin-4-one;

[0285] 7-((3R,4S)-4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)-3-fluoropiperidin-1-yl)-8,9-dimethyl-4H-pyrimido[1,2-b]pyridazin-4-one;

[0286] 7-((3S,4S)-4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)-3-fluoropiperidin-1-yl)-2-(methoxymethyl)-8,9-dimethyl-4H-pyrimido[1,2-b]pyridazin-4-one;

[0287] 7-((3S,4R)-4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)-3-fluoropiperidin-1-yl)-2-(methoxymethyl)-8,9-dimethyl-4H-pyrimido[1,2-b]pyridazin-4-one;

[0288] 7-((3R,4S)-4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)-3-fluoropiperidin-1-yl)-2-(methoxymethyl)-8,9-dimethyl-4H-pyrimido[1,2-b]pyridazin-4-one;

[0289] 7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl)oxy)piperidin-1-yl)-8-methyl-9-(methylamino)-4H-pyrimido[1,2-b]pyridazin-4-one;

[0290] 7-((3R,4R)-4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)-3-fluoropiperidin-1-yl)-2-(methoxymethyl)-8,9-dimethyl-4H-pyrimido[1,2-b]pyridazin-4-one;

[0291] 7-((3R,4R)-4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)-3-fluoropiperidin-1-yl)-2-(methoxymethyl)-8-methyl-4H-pyrimido[1,2-b]pyridazin-4-one;

[0292] 7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl)oxy)piperidin-1-yl)-9-methoxy-8-methyl-4H-pyrimido[1,2-b]pyridazin-4-one;

[0293] 9-(azetidin-1-yl)-7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl)oxy)piperidin-1-yl)-8-methyl-4H-pyrimido[1,2-b]pyridazin-4-one;

[0294] 7-((3R,4R)-4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)-3-fluoropiperidin-1-yl)-8,9-dimethyl-4H-pyrimido[1,2-b]pyridazin-4-one;

[0295] 7-((2R,4S)-4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)-2-methylpiperidin-1-yl)-8-methyl-4H-pyrimido[1,2-b]pyridazin-4-one;

[0296] 7-((2R,4S)-4-((8-fluoro-2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)-2-methylpiperidin-1-yl)-8-methyl-4H-pyrimido[1,2-b]pyridazin-4-one;

[0297] 7-((2R,4S)-4-((8-fluoro-2,3-dihydrobenzo[b][1,4]dioxin-6-yl)oxy)-2-methylpiperidin-1-yl)-8-methyl-4H-pyrimido[1,2-b]pyridazin-4-one;

[0298] 7-((2R,4S)-4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)-2-methylpiperidin-1-yl)-8,9-dimethyl-4H-pyrimido[1,2-b]pyridazin-4-one;

[0299] 7-((2R,4S)-4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)-2-methylpiperidin-1-yl)-2-(methoxymethyl)-8,9-dimethyl-4H-pyrimido[1,2-b]pyridazin-4-one;

[0300] 7-((2R,4S)-4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl)oxy)-2-methylpiperidin-1-yl)-8,9-dimethyl-4H-pyrimido[1,2-b]pyridazin-4-one;

[0301] 7-(4-((8-fluoro-2,3-dihydrobenzo[b][1,4]dioxin-6-yl)oxy)piperidin-1-yl)-8-methyl-4H-pyrimido[1,2-b]pyridazin-4-one;

[0302] 7-(4-((8-fluoro-2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)piperidin-1-yl)-8-methyl-4H-pyrimido[1,2-b]pyridazin-4-one;

[0303] 7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)piperidin-1-yl-2,2,6,6-d4)-8-methyl-4H-pyrimido[1,2-b]pyridazin-4-one; or a pharmaceutically acceptable salt thereof.

[0304] E39. A pharmaceutical composition comprising the compound of any one of embodiments E1 to E38 and a pharmaceutically acceptable carrier.

[0305] E40. A method for treating a neurological and / or psychiatric disorder in a patient in need thereof, the method comprising administering to the patient a therapeutically effective amount of a compound as described in any one of Embodiments E1 to E39, or a pharmaceutically acceptable salt thereof, or a pharmaceutical composition.

[0306] E41. The method of embodiment E40, wherein the disorder is selected from Alzheimer's disease, schizophrenia, sleep disorders, borderline personality disorder, Tourette's syndrome, bipolar disorder, tardive dyskinesia, Huntington's disease, pain disorders and cognitive disorders.

[0307] E42. A method as described in embodiment E40, wherein the disorder is selected from psychosis, schizophrenia, conduct disorder, disruptive behavior disorder, bipolar disorder, anxious psychotic episodes, anxiety associated with psychosis, mood disorders associated with psychotic disorders, acute mania, depression associated with bipolar disorder, mood disorders associated with schizophrenia, behavioral manifestations of mental retardation, autistic disorders, movement disorders, Tourette syndrome, akinesia-rigidity syndrome, movement disorders associated with Parkinson's disease, tardive dyskinesia, movement difficulties based on drug-induced and neurodegeneration, attention deficit hyperactivity disorder, cognitive disorders, dementia and memory disorders.

[0308] E43. The method of any one of embodiments E40 to E42, wherein the disorder is associated with muscarinic acetylcholine receptor M4 dysfunction.

[0309] E44. A compound as described in any one of embodiments E1 to E38, or a pharmaceutically acceptable salt thereof, for use in therapy.

[0310] E45. A compound as described in any one of Embodiments E1 to E39, or a pharmaceutically acceptable salt thereof, or a pharmaceutical composition for use in treating a disorder selected from Alzheimer's disease, schizophrenia, sleep disorders, borderline personality disorder, Tourette's syndrome, bipolar disorder, tardive dyskinesia, Huntington's disease, pain disorders and cognitive disorders.

[0311] E46. A compound as described in any one of Embodiments E1 to E39, or a pharmaceutically acceptable salt thereof, or a pharmaceutical composition, for use in the treatment of a disorder selected from psychosis, schizophrenia, conduct disorder, disruptive behavior disorder, bipolar disorder, anxious psychotic episodes, anxiety associated with psychosis, mood disorders associated with psychotic disorders, acute mania, depression associated with bipolar disorder, mood disorders associated with schizophrenia, behavioral manifestations of mental retardation, autistic disorders, movement disorders, Tourette's syndrome, akinesia-rigidity syndrome, movement disorders associated with Parkinson's disease, tardive dyskinesia, movement difficulties based on drug-induced and neurodegeneration, attention deficit hyperactivity disorder, cognitive disorders, dementia and memory disorders.

[0312] E47. Use of a compound as described in any one of Embodiments E1 to E39, or a pharmaceutical composition, or a pharmaceutically acceptable salt thereof, in the manufacture of a medicament for treating a disorder selected from Alzheimer's disease, schizophrenia, borderline personality disorder, Tourette's syndrome, bipolar disorder, tardive dyskinesia, Huntington's disease, sleep disorders, pain disorders and cognitive disorders.

[0313] E48. Use of a compound as described in any one of Embodiments E1 to E39, or a pharmaceutical composition, or a pharmaceutically acceptable salt thereof, in the manufacture of a medicament for treating a disorder selected from psychosis, schizophrenia, conduct disorder, disruptive behavior disorder, bipolar disorder, anxious psychotic episodes, anxiety associated with psychosis, mood disorders associated with psychotic disorders, acute mania, depression associated with bipolar disorder, mood disorders associated with schizophrenia, behavioral manifestations of mental retardation, autistic disorders, movement disorders, Tourette's syndrome, akinesia-rigidity syndrome, movement disorders associated with Parkinson's disease, tardive dyskinesia, movement difficulties based on drug-induced and neurodegeneration, attention deficit hyperactivity disorder, cognitive disorders, dementia and memory disorders.

[0314] By using as The Struct=Name naming algorithm, which is part of Version 18.0, assigns compound names.

[0315] Compounds may exist as stereoisomers with asymmetric or chiral centers therein. The stereoisomers are "R" or "S" depending on the configuration of the substituents around the chiral carbon atom. The terms "R" and "S" as used herein are configurations as defined in IUPAC 1974 Recommendations for Section E, Fundamental Stereochemistry, Pure Appl. Chem., 1976, 45: 13-30. The present invention contemplates various stereoisomers and mixtures thereof, and these are specifically included within the scope of the present invention. Stereoisomers include enantiomers and diastereomers and mixtures of enantiomers or diastereomers. Individual stereoisomers of these compounds may be synthesized from commercially available starting materials containing asymmetric or chiral centers, or by preparing a racemic mixture followed by resolution methods well known to those of ordinary skill in the art. These resolution methods are exemplified by (1) attaching the mixture of enantiomers to a chiral auxiliary, separating the resulting diastereomeric mixture by recrystallization or chromatography and optionally liberating the optically pure product from the auxiliary as described in Furniss, Hannaford, Smith, and Tatchell, "Vogel's Textbook of Practical Organic Chemistry", 5th edition (1989), Longman Scientific & Technical, Essex CM20 2JE, England, or (2) directly separating the mixture of optical enantiomers on a chiral chromatographic column, or (3) fractional recrystallization methods.

[0316] It is to be understood that the compounds of the present invention may have tautomeric forms, stereoisomers, geometric isomers, solvates, hydrates and polymorphs, and these also constitute embodiments of the present invention.

[0317] In the compounds of formula (I) and any subformulae, any "hydrogen" or "H", whether explicitly listed or implicit in the structure, includes hydrogen isotopes 1 H (hydrogen) and 2 H (deuterium).

[0318] The present invention also includes isotopically labeled compounds, which are identical to those described in formula (I), but in fact one or more atoms are replaced by atoms with an atomic mass or mass number different from the atomic mass or mass number usually found in nature. Examples of isotopes suitable for inclusion in the compounds of the present invention are hydrogen, carbon, nitrogen, oxygen, phosphorus, sulfur, fluorine, and chlorine, such as but not limited to the corresponding 2 H. 13 C. 14 C. 15 N. 18 O. 17 O. 31 P. 32 P. 35 S. 18 F and 36 Cl. Use heavier isotopes such as deuterium (i.e. 2 H) substitution may result in certain therapeutic advantages resulting from greater metabolic stability (e.g., increased in vivo half-life or reduced dosage requirements) and may therefore be preferred in some circumstances. The compound may contain a positron emitting isotope for medical imaging and positron emission tomography (PET) studies for determining receptor distribution. Suitable positron emitting isotopes that may be incorporated into compounds of formula (I) are 11 C. 13 N. 15 O. and 18 F. Isotopically labeled compounds of formula (I) can generally be prepared by conventional techniques known to those skilled in the art or by processes analogous to those described in the accompanying Examples, using an appropriate isotopically labeled reagent in place of a non-isotopically labeled reagent.

[0319] a. Pharmaceutically acceptable salts

[0320] The compounds of the present invention may exist as pharmaceutically acceptable salts. The term "pharmaceutically acceptable salt" refers to salts or zwitterions of compounds that are water-soluble or oil-soluble or dispersible, suitable for treating disorders without abnormal toxicity, irritation and allergic reactions, commensurate with a reasonable benefit / risk ratio, and effective for the intended use. These salts can be prepared during the final separation and purification of the compound, or prepared separately by reacting the amino group of the compound with a suitable acid. For example, the compound can be dissolved in a suitable solvent (such as, but not limited to, methanol and water) and treated with at least one equivalent of an acid (such as hydrochloric acid). The resulting salt can be precipitated and separated by filtration and dried under reduced pressure. Alternatively, the solvent and excess acid can be removed under reduced pressure to provide a salt. Representative salts include acetate, adipate, alginate, citrate, aspartate, benzoate, benzenesulfonate, bisulfate, butyrate, camphorate, camphorsulfonate, digluconate, glycerophosphate, hemisulfate, heptanoate, hexanoate, formate, isethionate, fumarate, lactate, maleate, methanesulfonate, naphthylsulfonate, nicotinate, oxalate, pamoate, pectinate, persulfate, 3-phenylpropionate, picrate, oxalate, maleate, pivalate, propionate, succinate, tartrate, trichloroacetate, trifluoroacetate, glutamate, p-toluenesulfonate, undecanoate, hydrochloride, hydrobromide, sulfate, phosphate, etc. The amino groups of the compounds can be quaternized with alkyl chlorides, bromides, and iodides (e.g., methyl, ethyl, propyl, isopropyl, butyl, lauryl, myristyl, stearyl, etc.).

[0321] Base addition salts can be prepared during the final isolation and purification of the compounds of the invention by reacting the carboxyl group with a suitable base such as a hydroxide, carbonate or bicarbonate of a metal cation (e.g. lithium, sodium, potassium, calcium, magnesium or aluminum), or with an organic primary, secondary or tertiary amine. Quaternary ammonium salts can be prepared, for example, those derived from methylamine, dimethylamine, trimethylamine, triethylamine, diethylamine, ethylamine, tributylamine, pyridine, N,N-dimethylaniline, N-methylpiperidine, N-methylmorpholine, dicyclohexylamine, procaine, dibenzylamine, N,N-dibenzylphenethylamine, 1-diphenylhydroxymethylamine and N,N'-dibenzylethylenediamine, ethylenediamine, ethanolamine, diethanolamine, piperidine, piperazine and the like.

[0322] b. General synthesis

[0323] Compounds or salts thereof with formula (I) can be prepared by synthetic methods or metabolic methods. The preparation of compounds by metabolic methods includes those occurring in the human or animal body (in vivo) or in vitro. These schemes represent methods that can be used to synthesize the compounds of the present invention. They do not limit the scope of the present invention in any way.

[0324] Abbreviations used in the following schemes include the following: DCE is 1,2-dichloroethane; DCM is dichloromethane; DMF is N,N-dimethylformamide; DMP or Dess-Martin periodinane is 1,1,1-tris(acetoxy)-1,1-dihydro-1,2-benzidoyl-3-(1H)-one; DtBAD is di-tert-butyl azodicarboxylate; LAH is lithium aluminum hydride; mCPBA is meta-chloroperbenzoic acid; MeOH is methanol; MW is microwave (referring to microwave reactor); Pd(OAc)2 is palladium(II) acetate; PPA is polyphosphoric acid; Rxn is reaction; TBAC is tetrabutylammonium chloride; t-BuXPhos is 2-di-tert-butylphosphino-2′,4′,6′-triisopropylbiphenyl; and THF is tetrahydrofuran.

[0325] Compounds having formula (I) can be synthesized as shown in Schemes 1-7.

[0326] Solution 1

[0327]

[0328] As shown in Scheme 1, reaction of analogs of 6-chloropyridazin-3-amine with triethyl orthoformate and isopropylidene malonate can provide compounds of type S1, which can be cyclized to provide compounds of type S2. Reaction of compound S2 with appropriately substituted 4-piperidinyl ethers of type S3 in the presence of a base can provide compound S4.

[0329] Solution 2

[0330]

[0331] As shown in Scheme 2, reaction of analogs of 6-chloropyridazin-3-amine with β-keto acids can be cyclized with an acid (i.e., PPA) to provide compounds of type S2. Reaction of compound S2 with an appropriately substituted 4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl)oxy)piperidine of type S3 in the presence of a base can provide compound S4.

[0332] Solution 3

[0333]

[0334] As shown in Scheme 3, intermediates such as compound S3 can be prepared by displacing tert-butyl 4-((methylsulfonyl)oxy)piperidine-1-carboxylate with an appropriate alcohol (ie, compound i) to provide intermediate S5, which can be deprotected under acidic conditions to provide intermediate S3.

[0335] Solution 4

[0336]

[0337] As shown in Scheme 4, intermediates such as compound S3 can be prepared by Mitsunobu reaction between an analog of tert-butyl 4-hydroxypiperidine-1-carboxylate and an appropriate alcohol (ie, compound i) to provide intermediate S5, which can be deprotected under acidic conditions to provide intermediate S3.

[0338] Solution 5

[0339]

[0340] As shown in Scheme 5, 3,4-dihydroxybenzaldehyde and 1,2-dibromoethane-1,1,2,2-d4 react in the presence of a base (i.e., K2CO3) to afford intermediate S6. In a 2-step procedure, intermediate S6 is treated with m-CPBA and then by base and methanol to provide alcohol S7, which can be continued as shown in Scheme 4 to provide intermediate S8.

[0341] Solution 6

[0342]

[0343] As shown in Scheme 6, analogs of 6-chloropyridazin-3-amines S14 and S15 can be prepared by free radical based addition of appropriate carboxylic acids to provide intermediate S13, which can be transformed into the desired intermediates S14 and S15.

[0344] Compounds and intermediates can be separated and purified by methods well known to those skilled in the art of organic synthesis. Examples of conventional methods for separating and purifying compounds may include, but are not limited to, chromatography on a solid support (e.g., silica gel, alumina or silica derived from alkylsilane groups), by recrystallization at elevated or low temperatures (optionally pre-treated with activated carbon), thin layer chromatography, distillation at various pressures, sublimation under vacuum, and grinding, as described in, for example, "Vogel's Textbook of Practical Organic Chemistry," 5th edition (1989), Furniss, Hannaford, Smith, and Tatchell, Longman Scientific & Technical, Essex CM20 2JE, England.

[0345] The disclosed compounds may have at least one basic nitrogen, so that the compound can be treated with an acid to form a desired salt. For example, the compound can be reacted with an acid at or above room temperature to provide the desired salt, which is precipitated and collected by filtration after cooling.

[0346] The reaction conditions and reaction times of each individual step can vary according to the substituent present in the specific reactant adopted and the reactant used. The example part provides a specific program. The reaction can be handled in a conventional manner, for example, by removing the solvent from residue and further purified according to methods generally known in the art (such as but not limited to crystallization, distillation, extraction, grinding and chromatography). Unless otherwise indicated, starting material and reagent are commercially available, or can be prepared from commercially available materials by the method described in the chemical literature by those skilled in the art. Starting material (if not commercially available) can be prepared by the program selected from standard organic chemistry technology, the technology similar to the compound similar to the known structure of the synthesis or the technology similar to the program described in the above scheme or the synthesis example part.

[0347] Routine experimentation (including reaction conditions, reagents, the order of synthetic pathways, protection of any chemical functional groups incompatible with the reaction conditions, and appropriate manipulations of deprotection performed at appropriate points in the reaction sequence of the method) is included within the scope of the present invention. Suitable protecting groups and methods for protecting and deprotecting different substituents using such suitable protecting groups are well known to those skilled in the art; examples thereof can be found in PGM Wuts and TW Greene, Greene's Protective Groups in Organic Synthesis (4th edition), John Wiley & Sons, New York (2006), which is incorporated herein by reference in its entirety. The synthesis of the compounds of the present invention can be accomplished by methods similar to those described in the above-mentioned synthetic schemes and specific examples.

[0348] When an optically active form of a disclosed compound is required, it can be obtained by carrying out one of the procedures described herein using an optically active starting material (e.g. prepared by asymmetric induction of a suitable reaction step), or by resolving the mixture of stereoisomers of the compound or intermediates using standard procedures such as chromatographic separation, recrystallization or enzymatic resolution.

[0349] Similarly, when a pure geometric isomer of a compound is required, it can be obtained by carrying out one of the above procedures using a pure geometric isomer as starting material, or by resolving a mixture of geometric isomers of the compound or intermediates using standard procedures such as chromatographic separation.

[0350] It is understood that the synthetic schemes and specific examples are illustrative and should not be construed as limiting the scope of the invention as defined in the appended claims. All alternatives, modifications, and equivalents of the synthetic methods and specific examples are included within the scope of the claims.

[0351] c. Muscarinic acetylcholine receptor M4 activity

[0352] In embodiments, the compounds of the invention enhance the agonist response (e.g., acetylcholine) of mAChR M4. In some embodiments, the compounds of the invention increase the response of mAChR M4 to a non-maximal concentration of an agonist in the presence of the compounds of the invention compared to the response to the agonist in the absence of the compounds of the invention. The enhancement of mAChR M4 activity can be demonstrated by methods known in the art. For example, the response to Ca loading can be measured by measuring 2+ Activation of mAChR M4 activity is determined by measuring calcium flux in cells co-expressing an agonist (e.g., acetylcholine) and a chimeric or scrambled G protein with a sensitive fluorescent dye (e.g., Fluo-4). In embodiments, calcium flux is measured as an increase in the fluorescence static ratio. In embodiments, positive allosteric modulator activity is analyzed as EC 20 A concentration-dependent increase in the acetylcholine response (ie, the response of mAChR M4 at the acetylcholine concentration that produces 20% of the maximal response).

[0353] In the Examples, compounds of the invention activate mAChR M4 responses as an increase in calcium fluorescence of mAChR M4-transfected CHO-K1 cells in the presence of the compounds of the invention compared to the response of equivalent CHO-K1 cells in the absence of the compounds of the invention.

[0354] Compounds of the invention may demonstrate positive allosteric modulation of mAChR M4 in response to acetylcholine as an increase in response to non-maximal concentrations of acetylcholine in the presence of compound in CHO-K1 cells transfected with mAChR M4 compared to the response to acetylcholine in the absence of compound.

[0355] The in vivo efficacy of the compounds of the invention can be measured in a number of preclinical rat behavioral models, where known, clinically useful antipsychotic drugs show similar positive responses. For example, the compounds of the invention can reverse the excitatory movements induced by amphetamine in male Sprague-Dawley rats at oral doses ranging from 1 to 100 mg / kg.

[0356] 3. Pharmaceutical Composition

[0357] The compounds of the invention may be incorporated into pharmaceutical compositions suitable for administration to a subject (eg, a patient, which may be human or non-human). The compounds of the invention may also be provided as a formulation (eg, a spray-dried dispersion formulation).

[0358] The pharmaceutical composition may include a "therapeutically effective amount" or a "prophylactically effective amount" of a medicament. A "therapeutically effective amount" refers to an effective amount that is taken in a single dose or multiple doses and for a desired period of time to achieve the desired therapeutic outcome. The therapeutically effective amount of the composition can be determined by those skilled in the art and can vary according to a variety of factors, such as the individual's disease state, age, sex, and weight, and the ability of the composition to elicit a desired response in the individual. A therapeutically effective amount is also an amount in which any toxicity or adverse effects of the compounds of the present invention (e.g., a compound of formula (I) or a pharmaceutically acceptable salt thereof) do not exceed the amount of the therapeutically beneficial effect. A "prophylactically effective amount" refers to an effective amount that is taken in a dose and for a desired period of time to achieve the desired preventive outcome. Typically, because the preventive dose is used in the subject before or at an early stage of the disease, this preventive effective amount may be less than the therapeutically effective amount.

[0359] The pharmaceutical composition may include a pharmaceutically acceptable carrier. As used herein, the term "pharmaceutically acceptable carrier" means a non-toxic, inert solid, semi-solid or liquid filler, diluent, encapsulating material or formulation auxiliary of any type. Some examples of materials that can be used as pharmaceutically acceptable carriers are sugars such as, but not limited to, lactose, glucose, and sucrose; starches such as, but not limited to, corn starch and potato starch; cellulose and its derivatives such as, but not limited to, sodium carboxymethylcellulose, ethylcellulose, and cellulose acetate; powdered tragacanth; malt; gelatin; talc; excipients such as, but not limited to, cocoa butter and suppository waxes; oils such as, but not limited to, peanut oil, cottonseed oil, safflower oil, sesame oil, olive oil, corn oil, and soybean oil; glycols such as propylene glycol; esters such as, but not limited to, ethyl oleate and ethyl laurate; agar; buffers such as, but not limited to, magnesium hydroxide and aluminum hydroxide; alginic acid; pyrogen-free water; isotonic saline; Ringer's solution; ethanol, and phosphate buffered solutions, as well as other nontoxic compatible lubricants such as, but not limited to, sodium lauryl sulfate and magnesium stearate, and colorants, release agents, coating agents, sweeteners, flavoring agents, and perfuming agents, and preservatives and antioxidants may also be included in the composition at the discretion of the formulator.

[0360] Thus, the compounds of the invention can be formulated for administration by, for example, solid administration, eye drops, in topical oil-based formulations, injection, inhalation (by mouth or nose), implants, or for oral, buccal, parenteral, or rectal administration. Techniques and formulations generally can be found in Remington's Pharmaceutical Sciences, (Meade Publishing Co., Easton, Pa.). Therapeutic compositions typically must be sterile and stable under the conditions of manufacture and storage.

[0361] The route of administration of the compounds of the invention and the form of the composition will determine the type of carrier to be used. The composition can be in a variety of forms suitable for, for example, systemic administration (e.g., oral, rectal, nasal, sublingual, buccal, implant or parenteral) or local administration (e.g., skin, lung, nose, ear, eye, liposome delivery system, or iontophoresis).

[0362] Carriers for systemic administration typically include at least one of a diluent, a lubricant, a binder, a disintegrant, a colorant, a flavoring agent, a sweetener, an antioxidant, a preservative, a glidant, a solvent, a suspending agent, a wetting agent, a surfactant, a combination thereof, etc. All carriers are optional in the composition.

[0363] Suitable diluents include sugars such as glucose, lactose, dextrose and sucrose; glycols such as propylene glycol; calcium carbonate; sodium carbonate; sugar alcohols such as glycerol; mannitol; and sorbitol. The amount of one or more diluents in the systemic or topical composition is typically about 50 to about 90 weight percent of the total composition weight.

[0364] Suitable lubricants include silicon dioxide, talc, stearic acid and its magnesium and calcium salts, calcium sulfate; and liquid lubricants such as polyethylene glycol and vegetable oils, such as peanut oil, cottonseed oil, sesame oil, olive oil, corn oil and cocoa butter. The amount of one or more lubricants in the systemic or topical composition is typically about 5% to about 10% by weight of the total composition.

[0365] Suitable binders include polyvinyl pyrrolidone; magnesium aluminum silicate; starches, such as corn starch and potato starch; gelatin; tragacanth; and cellulose and its derivatives, such as sodium carboxymethylcellulose, ethylcellulose, methylcellulose, microcrystalline cellulose and sodium carboxymethylcellulose. The amount of one or more binders in the systemic composition is typically from about 5% to about 50% by weight of the total composition.

[0366] Suitable disintegrants include agar, alginic acid and its sodium salt, effervescent mixtures, cross-linked carboxymethylcellulose, crospovidone, sodium carboxymethyl starch, sodium starch glycolate, clays and ion exchange resins. The amount of one or more disintegrants in the systemic or topical composition is typically about 0.1% to about 10% by weight of the total composition.

[0367] Suitable coloring agents include colorants such as FD&C dyes.When used, the amount of coloring agent in the systemic or topical compositions is typically from about 0.005% to about 0.1% by weight of the total composition.

[0368] Suitable flavoring agents include menthol, mint and fruit flavors.When used, the amount of one or more flavoring agents in the systemic or topical compositions is typically from about 0.1% to about 1.0% by weight of the total composition.

[0369] Suitable sweeteners include aspartame and saccharin.The amount of one or more sweeteners in the systemic or topical compositions is typically from about 0.001% to about 1% by weight of the total composition.

[0370] Suitable antioxidants include butylated hydroxyanisole ("BHA"), butylated hydroxytoluene ("BHT"), and vitamin E. The amount of one or more antioxidants in the systemic or topical compositions is typically from about 0.1% to about 5% by weight of the total composition.

[0371] Suitable preservatives include benzalkonium chloride, methylparaben and sodium benzoate.The amount of one or more preservatives in the systemic or topical compositions is typically from about 0.01% to about 5% by weight of the total composition.

[0372] Suitable glidants include silicon dioxide.The amount of one or more glidants in the systemic or topical compositions is typically from about 1% to about 5% by weight of the total composition.

[0373] Suitable solvents include water, isotonic saline, ethyl oleate, glycerol, hydroxylated castor oil, alcohols (e.g., ethanol) and phosphate buffered saline. The amount of one or more solvents in the systemic or topical compositions is typically from about 0% to about 100% by weight of the total composition.

[0374] Suitable suspending agents include AVICEL RC-591 (available from FMC Corporation, Philadelphia, Pennsylvania) and sodium alginate.The amount of one or more suspending agents in the systemic or topical compositions is typically from about 1% to about 8% by weight of the total composition.

[0375] Suitable surfactants include lecithin, polysorbate 80 and sodium lauryl sulfate, and TWEENS (from Atlas Powder Company, Wilmington, Delaware). Suitable surfactants include those disclosed in CTFA Cosmetic Ingredient Handbook, 1992, pp. 587-592; Remington's Pharmaceutical Sciences, 22nd ed. 2013; and McCutcheon's Volume 1, Emulsifiers & Detergents, 1994, North American edition, pp. 236-239. The amount of one or more surfactants in the systemic or topical composition is typically from about 0.1% to about 5% by weight of the total composition.

[0376] Although the amounts of the components in systemic compositions may vary depending on the type of systemic composition being prepared, generally, systemic compositions comprise 0.01 to 50% by weight of the active compound (e.g., a compound of formula (I) or a pharmaceutically acceptable salt thereof) and 50 to 99.99% by weight of one or more carriers, based on the weight of the total composition. Compositions for parenteral administration typically comprise 0.1 to 10% by weight of the active ingredient and 90 to 99.9% by weight of the carrier (including diluents and solvents), based on the weight of the total composition.

[0377] Compositions for oral administration can have various dosage forms. For example, solid forms include tablets, capsules, granules and bulk powders. These oral dosage forms include safe and effective amounts (generally at least about 5% by weight of the total composition weight and more particularly about 25 to about 50% by weight of the total composition weight) of active ingredient. These oral dosage compositions include about 50 to about 95% by weight of the total composition weight of the carrier and more particularly the total composition weight of the carrier from about 50 to about 75% by weight.

[0378] Tablets can be compressed, tablet grinding, enteric coating, sugar coating, film coating or multiple compression. Tablets typically include active ingredients and carriers, and the carrier includes a composition selected from diluents, lubricants, binders, disintegrants, colorants, flavorings, sweeteners, glidants and combinations thereof. Specific diluents include calcium carbonate, sodium carbonate, mannitol, lactose and cellulose. Specific binders include starch, gelatin and sucrose. Specific disintegrants include alginic acid and cross-linked carboxymethyl cellulose. Specific lubricants include magnesium stearate, stearic acid and talc. Specific coloring agents are FD&C dyes, which can be added for appearance. Chewable tablets preferably contain sweeteners (such as aspartame and saccharin) or flavorings (such as menthol, mint, fruit flavorings), or a combination thereof.

[0379] Capsules (including implants, timed release and sustained release formulations) typically include an active compound (e.g., a compound of formula (I)) and a carrier including one or more diluents in a gelatin-containing capsule disclosed above. The particles typically include the disclosed compound, preferably a glidant (e.g., silicon dioxide) to improve flow properties. Implants may be biodegradable or non-biodegradable types.

[0380] The choice of ingredients in the carrier for oral compositions depends on secondary considerations such as taste, cost and storage stability, which are not critical for the purposes of the present invention.

[0381] Solid compositions can be coated by conventional methods (typically with pH or time-dependent coatings) so that the disclosed compounds are released in the gastrointestinal tract near the desired administration, or at different points and times, to prolong the desired effect. The coating typically includes one or more components selected from the group consisting of cellulose acetate phthalate, polyvinyl acetate phthalate, hydroxypropyl methylcellulose phthalate, ethyl cellulose, Coating (obtained from Evonik Industries, Essen, Germany), wax, and shellac.

[0382] Compositions for oral administration can have liquid form.For example, suitable liquid forms include aqueous solutions, emulsions, suspensions, solutions reconstructed by non-effervescent particles, suspensions reconstructed by non-effervescent particles, effervescent preparations reconstructed by effervescent particles, elixirs, tinctures, syrups, etc. The compositions for oral administration of liquids typically include disclosed compounds and carriers, i.e. carriers selected from diluents, colorants, flavorings, sweeteners, preservatives, solvents, suspending agents and surfactants. Oral liquid compositions preferably include one or more components selected from colorants, flavorings and sweeteners.

[0383] Other compositions for achieving systemic delivery of the subject compound include sublingual dosage forms, oral dosage forms and nasal dosage forms. Such compositions typically include one or more soluble filler materials, such as diluents, including sucrose, sorbitol and mannitol; and binders, such as gum arabic, microcrystalline cellulose, carboxymethyl cellulose and hydroxypropyl methylcellulose. Such compositions may further include lubricants, colorants, flavorings, sweeteners, antioxidants and glidants.

[0384] The compounds of the present invention can be applied topically. Topical compositions that can be topically administered to the skin can be in any form, including solids, solutions, oils, creams, ointments, gels, lotions, shampoos, leave-on and rinse-off conditioners, milks, cleansers, moisturizers, sprays, skin patches, etc. Topical compositions include: a disclosed compound (e.g., a compound of formula (I) or a pharmaceutically acceptable salt thereof) and a carrier. The carrier of the topical composition preferably helps the compound penetrate into the skin. The carrier may further include one or more optional components.

[0385] The amount of carrier used in combination with the disclosed compounds is sufficient to provide a practical amount of the composition to be administered per unit dose of the compound. Techniques and compositions for preparing dosage forms useful in the methods of the present invention are described in the following references: Modern Pharmaceutics, Chapters 9 and 10, Banker & Rhodes, eds. (1979); Lieberman et al., Pharmaceutical Dosage Forms: Tablets (1981); and Ansel, Introduction to Pharmaceutical Dosage Forms, 2nd ed., (1976).

[0386] The carrier may include a single component or a combination of two or more components. In topical compositions, the carrier includes a topical carrier. Suitable topical carriers include one or more components selected from the group consisting of phosphate buffered saline, isotonic water, deionized water, monofunctional alcohols, symmetric alcohols, aloe vera gel, allantoin, glycerin, vitamin A and E oils, mineral oil, propylene glycol, PPG-2 myristyl propionate, dimethyl isosorbide, castor oil, combinations thereof, and the like. More specifically, carriers for skin administration include propylene glycol, dimethyl isosorbide and water, and even more specifically, phosphate buffered saline, isotonic water, deionized water, monofunctional alcohols and symmetric alcohols.

[0387] The carrier of the topical composition may further include one or more ingredients selected from the group consisting of emollients, propellants, solvents, humectants, thickeners, powders, fragrances, pigments and preservatives, all of which are optional.

[0388] Suitable emollients include stearyl alcohol, glyceryl monoricinoleate, glyceryl monostearate, propane-1,2-diol, butane-1,3-diol, mink oil, cetyl alcohol, isopropyl isostearate, stearic acid, isobutyl palmitate, isocetyl stearate, oleyl alcohol, isopropyl laurate, hexyl laurate, decyl oleate, octadecane-2-ol, isocetyl alcohol, cetyl palmitate, di-n-butyl sebacate, isopropyl myristate, isopropyl palmitate, isopropyl stearate, butyl stearate, polyethylene glycol, triethylene glycol, lanolin, sesame oil, coconut oil, peanut oil, castor oil, acetylated lanolin alcohol, petroleum, mineral oil, butyl myristate, isostearic acid, palmitic acid, isopropyl linoleate, lauryl lactate, myristyl lactate, decyl oleate, myristyl myristate, and combinations thereof. Specific emollients for use on the skin include stearyl alcohol and dimethicone.The amount of one or more emollients in the topical skin-based composition is typically from about 5 to about 95 weight percent of the total composition.

[0389] Suitable propellants include propane, butane, isobutane, dimethyl ether, carbon dioxide, nitrous oxide, and combinations thereof.The amount of one or more propellants in the topical composition is typically from about 0 to about 95 weight percent of the total composition weight.

[0390] Suitable solvents include water, ethanol, methylene chloride, isopropanol, castor oil, ethylene glycol monoethyl ether, diethylene glycol monobutyl ether, diethylene glycol monoethyl ether, dimethyl sulfoxide, dimethylformamide, tetrahydrofuran, and combinations thereof. Specific solvents include ethanol and homotonic alcohols. The amount of one or more solvents in the topical composition is typically from about 0 to about 95 weight percent of the total composition weight.

[0391] Suitable humectants include glycerin, sorbitol, sodium 2-pyrrolidone-5-carboxylate, soluble collagen, dibutyl phthalate, gelatin and combinations thereof. Specific humectants include glycerin. The amount of one or more humectants in the topical composition is typically 0 to 95 weight % of the total composition weight.

[0392] The amount of one or more thickeners in the topical composition is typically from about 0 to about 95 weight percent of the total composition.

[0393] Suitable powders include beta-cyclodextrin, hydroxypropyl cyclodextrin, chalk, talc, fuller's earth, kaolin, starch, gum, colloidal silica, sodium polyacrylate, tetraalkyl ammonium montmorillonite, trialkyl aryl ammonium montmorillonite, chemically modified magnesium aluminum silicate, organically modified montmorillonite clay, hydrated aluminum silicate, fumed silica, carboxyvinyl polymer, sodium carboxymethylcellulose, ethylene glycol monostearate, and combinations thereof. The amount of one or more powders in the topical composition is typically 0 to 95 weight percent of the total composition weight.

[0394] The amount of fragrance in the topical compositions is typically from about 0 to about 0.5 weight %, specifically from about 0.001 to about 0.1 weight %, based on the weight of the total composition.

[0395] Suitable pH adjusting additives include HCl or NaOH in an amount sufficient to adjust the pH of the topical pharmaceutical composition.

[0396] 4. Usage

[0397] The compounds and pharmaceutical compositions of the present invention can be used in methods for treating disorders associated with muscarinic acetylcholine receptor dysfunction (such as neurological disorders and / or psychiatric disorders). The compounds and pharmaceutical compositions of the present invention can also be used in methods for enhancing the activity of muscarinic acetylcholine receptors in patients, as well as methods for enhancing cognition in patients. The methods further include synergistic treatment methods in the context of cognitive or behavioral therapy to improve treatment outcomes. In the methods of use described herein, one or more additional therapeutic agents can be administered simultaneously or sequentially with the compounds and compositions of the present invention.

[0398] a. Treatment barriers

[0399] The compounds, pharmaceutical compositions and formulations of the present invention can be used in methods for treating disorders associated with muscarinic acetylcholine receptor dysfunction (e.g., neurological disorders and / or psychiatric disorders). The treatment method may include administering to a subject in need of such treatment a therapeutically effective amount of a compound of formula (I), a pharmaceutically acceptable salt thereof, or a pharmaceutical composition comprising a therapeutically effective amount of a compound of formula (I) or a pharmaceutically acceptable salt thereof.

[0400] In some embodiments, the present invention provides a method for enhancing cognition in a patient, comprising administering to the patient a therapeutically effective amount of a compound of formula (I), a pharmaceutically acceptable salt thereof, or a pharmaceutical composition comprising a therapeutically effective amount of a compound of formula (I) or a pharmaceutically acceptable salt thereof.

[0401] The compounds and compositions of the invention disclosed herein can be used to treat, prevent, ameliorate, control or reduce the risk of a variety of disorders associated with selective mAChR M4 receptor activation. For example, treatment can include selective mAChR M4 receptor activation to an extent effective to affect cholinergic activity. The disorder can be associated with cholinergic activity, such as cholinergic hypofunction. Therefore, a method for treating or preventing a disorder in a subject is provided, the method comprising the steps of administering to the subject at least one compound of the invention or at least one disclosed pharmaceutical composition in an amount effective to treat the disorder in the subject.

[0402] Also provided is a method for treating one or more disorders associated with mAChR M4 receptor activity in a subject, the method comprising the steps of administering to the subject a therapeutically effective amount of a compound of formula (I), a pharmaceutically acceptable salt thereof, or a pharmaceutical composition comprising a therapeutically effective amount of a compound of formula (I) or a pharmaceutically acceptable salt thereof.

[0403] In some embodiments, the present invention provides a compound of formula (I), or a pharmaceutically acceptable salt thereof, for use in a method of treating a disorder associated with the mAChR M4 receptor. In some embodiments, the present invention provides a pharmaceutical composition comprising a compound of formula (I), or a pharmaceutically acceptable salt thereof, for use in a method of treating a disorder associated with the mAChR M4 receptor.

[0404] In some embodiments, the present invention provides a compound having formula (I), or a pharmaceutically acceptable salt thereof, for use in the manufacture of a medicament for treating a disorder associated with the mAChR M4 receptor.

[0405] In some embodiments, the present invention provides a method for treating a disorder associated with muscarinic acetylcholine receptor dysfunction in a patient, the method comprising the step of administering to the patient an effective amount of at least one disclosed compound or a pharmaceutically acceptable salt thereof, or a pharmaceutical composition comprising at least one disclosed compound or a pharmaceutically acceptable salt thereof.

[0406] In some embodiments, the compounds and compositions of the present invention can be used to treat a variety of neurological, psychiatric and cognitive disorders associated with mAChR M4 receptors, including one or more of the following conditions or diseases: schizophrenia, psychotic disorder NOS, brief psychotic disorder, schizophreniform disorder, schizoaffective disorder, delusional disorder, shared psychotic disorder, catastrophic schizophrenia, postpartum psychosis, psychotic depression, mental breakdown, late onset psychosis, myxedema psychosis, occupational psychosis, catastrophic psychosis, secondary psychotic disorder, bipolar I disorder with psychotic features, and substance-induced psychotic disorder. In some embodiments, the psychotic disorder is a psychotic disorder associated with a disease selected from major depressive disorder, affective disorder, bipolar disorder, electrolyte imbalance, Alzheimer's disease, neurological disorder, hypoglycemia, AIDS, lupus, and post-traumatic stress disorder.

[0407] In some embodiments, the disorder is schizophrenia.

[0408] In some embodiments, the disorder is psychotic depression.

[0409] In some embodiments, the disorder is agitation and psychosis in Alzheimer's disease.

[0410] In some embodiments, the disorder is bipolar disorder.

[0411] In some embodiments, the disorder is a neurological disorder selected from a brain tumor, Lewy body dementia, multiple sclerosis, sarcoidosis, Lyme disease, syphilis, Alzheimer's disease, Parkinson's disease, and anti-NMDA receptor encephalitis.

[0412] In some embodiments, the disorder is a mental disorder selected from the group consisting of schizophrenia, brief psychotic disorder, schizophreniform disorder, schizoaffective disorder, delusional disorder, and shared psychotic disorder. In some embodiments, schizophrenia is selected from catastrophic schizophrenia, catatonic schizophrenia, paranoid schizophrenia, residual schizophrenia, disorganized schizophrenia, and undifferentiated schizophrenia. In some embodiments, the disorder is selected from schizoid personality disorder, schizotypal personality disorder, and paranoid personality disorder. In some embodiments, the mental disorder is due to a general medical condition and is substance-induced or drug-induced (phencyclidine, ketamine and other dissociative anesthetics, amphetamines and other psychostimulant drugs, and cocaine).

[0413] In some embodiments, the present invention provides a method for treating a neurodevelopmental disorder, comprising administering an effective amount of a compound, or a pharmaceutically acceptable salt, or a pharmaceutical composition of the present invention to a patient in need thereof. In some embodiments, the neurodevelopmental disorder is fragile X syndrome.

[0414] In some embodiments, the present invention provides a method for treating cognitive impairment, the method comprising administering an effective amount of a compound of the present invention, or a pharmaceutically acceptable salt, or a pharmaceutical composition to a patient in need thereof. In some embodiments, cognitive impairment includes dementia (associated with Alzheimer's disease, ischemia, multi-infarct dementia, trauma, vascular problems or stroke, HIV disease, Parkinson's disease, Huntington's disease, Pick's disease, Creutzfeldt-Jakob disease, perinatal hypoxia, other general medical conditions or substance abuse), delirium, amnesia, substance-induced persistent delirium, dementia caused by HIV disease, dementia caused by Huntington's disease, dementia caused by Parkinson's disease, Parkinson's syndrome-ALS dementia complex, Alzheimer's dementia, age-related cognitive decline and mild cognitive impairment.

[0415] In some embodiments, the cognitive disorder is Huntington's disease.

[0416] The text revision of the fourth edition of the Diagnostic and Statistical Manual of Mental Disorders (DSM-IV-TR) (2000, American Psychiatric Association, Washington, DC) provides a diagnostic tool that includes cognitive disorders (including dementia, delirium, amnesia, and age-related cognitive decline). The fifth edition of the Diagnostic and Statistical Manual of Mental Disorders (DSM-5) (2013, American Psychiatric Association, Washington, DC) provides a diagnostic tool for neurocognitive disorders (NCDs) that includes delirium, followed by major NCDs, minor NCDs, and their etiological subtype syndromes. Severe or mild NCD subtypes include NCD caused by Alzheimer's disease, vascular NCD, Lewy body NCD, NCD caused by Parkinson's disease, frontotemporal lobe NCD, NCD caused by traumatic brain injury, NCD caused by HIV infection, substance / drug-induced NCD, NCD caused by Huntington's disease, NCD caused by prion disease, NCD caused by other medical conditions, NCD caused by multiple causes and unspecified NCD. The NCD category in DSM-5 includes a group of disorders, in which the main clinical defect is cognitive function, and is acquired rather than developmental. As used herein, the term "cognitive disorder" includes the treatment of those cognitive disorders and mental cognitive disorders as described in DSM-IV-TR or DSM-5. Those skilled in the art will recognize that there are alternative nomenclatures, nosology and classification systems for mental disorders, and these systems develop along with medical and scientific progress. Therefore, the term "cognitive disorder" is intended to include similar disorders described in other diagnostic sources.

[0417] In some embodiments, the present invention provides a method for treating a sleep disorder, comprising administering an effective amount of a compound of the present invention, or a pharmaceutically acceptable salt thereof, or a pharmaceutical composition thereof to a patient in need thereof.

[0418] In some embodiments, the sleep disturbance is associated with sleep disturbance in patients with schizophrenia.

[0419] In some embodiments, the present invention provides a method for treating schizophrenia or psychosis, the method comprising administering an effective amount of a compound of the present invention, or a pharmaceutically acceptable salt, or a pharmaceutical composition to a patient in need thereof. Specific schizophrenia or psychotic disorders are paranoid, disorganized, catatonic or undifferentiated schizophrenia and substance-induced psychotic disorders. DSM-IV-TR provides a diagnostic tool including paranoid, disorganized, catatonic, undifferentiated or residual schizophrenia and substance-induced psychotic disorders. DSM-5 eliminates the subtypes of schizophrenia and instead includes a dimensional approach to assess the severity of the core symptoms of schizophrenia to capture the heterogeneity of the symptom types and severity exhibited in individuals with mental disorders. As used herein, the term "schizophrenia or psychosis" includes treatments for those mental disorders as described in DSM-IV-TR or DSM-5. Those skilled in the art will recognize that there are alternative nomenclatures, nosologies, and classification systems for mental disorders, and that these systems develop as medicine and science advance. Thus, the term "schizophrenia or psychosis" is intended to include similar disorders described in other diagnostic sources.

[0420] In some embodiments, the invention provides a method for treating pain, the method comprising administering a therapeutically effective amount of a compound or composition of the invention to a patient in need thereof. Specific pain examples are bone and joint pain (osteoarthritis), repetitive motion pain, dental pain, cancer pain, myofascial pain (muscle injury, fibromyalgia), perioperative pain (general surgery, gynecological surgery), chronic pain, and neuropathic pain.

[0421] These compounds and compositions can be further used in the following methods, which are used to prevent, treat, control, improve or reduce the risk of the diseases, disorders and conditions described herein. The compounds and compositions of the present invention can be further combined with other agents for use in the following methods, which are used to prevent, treat, control, improve or reduce the risk of the above-mentioned diseases, disorders and conditions.

[0422] In the treatment of a condition requiring activation of mAChR M4, an appropriate dosage level may be from about 0.001 mg / kg to about 100 mg / kg of patient body weight / day, which may be administered in a single dose or multiple doses. In particular, the daily dose may be in the range of from 0.01 mg / kg body weight to about 50 mg / kg body weight per day. The dosage level may be from about 0.1 to about 30 mg / kg / day, or more particularly from about 1 to about 10 mg / kg / day. A suitable dosage level may be from about 0.01 to 250 mg / kg per day, from about 0.05 to 100 mg / kg per day, or from about 0.1 to 50 mg / kg per day. Within this range, the dosage may be from 0.05 to 0.5, 0.5 to 5, or 5 to 50 mg / kg per day. For oral administration, the composition can be provided in the form of tablets containing 1.0 to 1000 mg of active ingredient, specifically 1.0, 5.0, 10, 15, 20, 25, 50, 75, 100, 150, 200, 250, 300, 400, 500, 600, 750, 800, 900 or 1000 mg of active ingredient for dosage adjustment of the symptoms of the patient being treated. The compound can be administered in a regimen of 1 to 4 times a day, preferably once or twice a day. The dosage regimen can be adjusted to provide the best therapeutic response. However, it should be understood that the specific dosage level and frequency of administration for any particular patient can vary and depend on a variety of factors, including the activity of the specific compound used, the metabolic stability and duration of action of the compound, age, body weight, general health, sex, diet, mode and time of administration, excretion rate, drug combination, severity of a particular disease, and the host receiving treatment.

[0423] Thus, in some embodiments, the present invention relates to a method for activating mAChR M4 receptor activity in at least one cell, the method comprising the steps of contacting the at least one cell with at least one compound or pharmaceutical composition of the present invention in an amount effective to activate mAChR M4 in the at least one cell. In some embodiments, the cell is mammalian, such as human. In some embodiments, the cell has been isolated from a subject prior to the contacting step. In some embodiments, the contacting is performed by administering to a subject.

[0424] In some embodiments, the present invention relates to a method for activating mAChR M4 activity in a subject, the method comprising the steps of administering to the subject at least one compound of the present invention or at least one product of the disclosed method at a dose and amount effective to activate mAChR M4 activity in the subject. In some embodiments, the subject is a patient. In some embodiments, prior to the administering step, the patient has been diagnosed as needing mAChR M4 agonism. In some embodiments, prior to the administering step, the patient has been diagnosed as needing mAChR M4 activation. In some embodiments, the method further comprises the steps of identifying a subject in need of mAChR M4 agonism.

[0425] In some embodiments, the present invention relates to a method for treating a disorder associated with selective mAChR M4 activation (e.g., a disease disorder associated with cholinergic activity) in a patient, the method comprising the steps of administering to the patient at least one compound of the present invention or at least one product of the disclosed method in a dose and amount effective to treat the disorder in the patient. In some embodiments, the patient is a human. In some embodiments, prior to the administering step, the patient has been diagnosed as needing treatment for the disorder. In some embodiments, the method further comprises the steps of identifying a subject in need of treatment for the disorder.

[0426] In some embodiments, the disorder can be selected from psychosis, schizophrenia, conduct disorder, disruptive behavior disorder, bipolar disorder, anxious psychotic episodes, anxiety associated with psychosis, psychotic mood disorders, such as severe major depressive disorder; mood disorders associated with psychotic disorders, acute mania, depression associated with bipolar disorder, mood disorders associated with schizophrenia, behavioral manifestations of mental retardation, autistic disorders, movement disorders, Tourette's syndrome, akinesia-rigidity syndrome, movement disorders associated with Parkinson's disease, tardive dyskinesia, movement difficulties based on drug-induced and neurodegeneration, attention deficit hyperactivity disorder, obsessive-compulsive disorder, cognitive disorders, dementia and memory disorders.

[0427] In some embodiments, the disorder is Alzheimer's disease.

[0428] In some embodiments, the disorder is Tourette's syndrome.

[0429] In some embodiments, the disorder is tardive dyskinesia.

[0430] In some embodiments, the disorder is obsessive compulsive disorder.

[0431] In some embodiments, the disorder is bipolar disorder.

[0432] b. Enhancement of muscarinic acetylcholine receptor activity

[0433] In some embodiments, the present invention relates to a method for enhancing muscarinic acetylcholine receptor activity in a patient, comprising the step of administering to the patient an effective amount of at least one disclosed compound or a pharmaceutically acceptable salt thereof, or a pharmaceutical composition comprising at least one disclosed compound or a pharmaceutically acceptable salt thereof.

[0434] In some embodiments, the present invention provides a compound of formula (I), or a pharmaceutically acceptable salt thereof, for use in a method of enhancing the activity of a muscarinic acetylcholine receptor in a patient. In some embodiments, the present invention provides a pharmaceutical composition comprising a compound of formula (I), or a pharmaceutically acceptable salt thereof, for use in a method of enhancing the activity of a muscarinic acetylcholine receptor in a patient.

[0435] In some embodiments, the present invention provides a compound of formula (I), or a pharmaceutically acceptable salt thereof, for use in the manufacture of a medicament for enhancing muscarinic acetylcholine receptor activity in a patient.

[0436] In some embodiments, the enhancement of muscarinic acetylcholine receptor activity increases muscarinic acetylcholine receptor activity. In some embodiments, the enhancement of muscarinic acetylcholine receptor activity is partial agonism of muscarinic acetylcholine receptors. In some embodiments, the enhancement of muscarinic acetylcholine receptor activity is positive allosteric modulation of muscarinic acetylcholine receptors.

[0437] In some embodiments, enhancement of muscarinic acetylcholine receptor activity in a patient is relevant to treating a neurological and / or psychiatric disorder associated with muscarinic receptor dysfunction, such as a neurological or psychiatric disorder disclosed herein. In some embodiments, the muscarinic receptor is mAChR M4.

[0438] c. Enhance cognition

[0439] In some embodiments, the present invention relates to a method for enhancing cognition in a patient, comprising the step of administering to the patient an effective amount of at least one disclosed compound of the present invention.

[0440] In some embodiments, the present invention provides a compound of formula (I), or a pharmaceutically acceptable salt thereof, for use in a method of enhancing patient cognition. In some embodiments, the present invention provides a pharmaceutical composition comprising a compound of formula (I), or a pharmaceutically acceptable salt thereof, for use in a method of enhancing patient cognition.

[0441] In some embodiments, the present invention provides a compound having formula (I), or a pharmaceutically acceptable salt thereof, for use in the manufacture of a medicament for enhancing cognition in a patient.

[0442] In some embodiments, prior to the administering step, the patient has been diagnosed as being in need of cognitive enhancement. In some embodiments, the method further comprises the step of identifying a patient in need of cognitive enhancement. In some embodiments, the need for cognitive enhancement is associated with muscarinic receptor dysfunction. In some embodiments, the muscarinic receptor is mAChR M4.

[0443] Cognitive deficit or cognitive impairment includes a decline in cognitive function or cognitive domain, such as working memory, attention and vigilance, verbal learning and memory, visual learning and memory, reasoning and problem solving (such as executive function), processing speed and / or social cognition. In particular, cognitive deficit or cognitive impairment may indicate attention deficit, confusion, slow thinking, difficulty in understanding, poor attention, impaired problem-solving ability, poor memory, difficulty in expressing thoughts and / or integrating thoughts, feelings and behaviors, or difficulty in eliminating irrelevant thoughts. The terms "cognitive deficit" and "cognitive impairment" are intended to indicate the same meaning and can be used interchangeably.

[0444] As mentioned above, cognitive function is often impaired in schizophrenia patients. Studies have also concluded that cognitive function is related to occupational function in schizophrenia [Scizophrenia Res., 45, 175-184, 2000]. In one embodiment, the patient to be treated for cognitive impairment is a schizophrenia patient.

[0445] The skilled artisan is familiar with various tests for measuring cognitive enhancement. Examples of tests for measuring cognitive enhancement are, but are not limited to, novel object recognition and the Wisconsin Card Sorting Test.

[0446] d. Co-treatment methods

[0447] The present invention further relates to the administration of selective mAChR M4 activators for improving treatment outcomes in the context of cognitive or behavioral therapy. That is, in some embodiments, the present invention relates to a co-therapeutic method comprising the steps of administering to a patient an effective amount and dosage of a compound of the present invention.

[0448] In some embodiments, the present invention provides a compound of formula (I), or a pharmaceutically acceptable salt thereof, for use in a synergistic treatment method for cognitive or behavioral therapy of a patient. In some embodiments, the present invention provides a pharmaceutical composition comprising a compound of formula (I), or a pharmaceutically acceptable salt thereof, for use in a synergistic treatment method for cognitive or behavioral therapy of a patient.

[0449] In some embodiments, the present invention provides a compound of formula (I), or a pharmaceutically acceptable salt thereof, for use in the manufacture of a medicament for a co-treatment method of cognitive or behavioral therapy of a patient.

[0450] In certain embodiments, in the context of cognitive or behavioral therapy, administration improves therapeutic effect. Administration combined with cognitive or behavioral therapy can be continuous or intermittent. Administration does not need to be carried out simultaneously with therapy, and can be carried out before, during and / or after therapy. For example, cognitive or behavioral therapy can be provided within 1, 2, 3, 4, 5, 6, 7 days before or after the administration of the compound. As another example, cognitive or behavioral therapy can be provided within 1, 2, 3 or 4 weeks before or after the administration of the compound. As another example still, cognitive or behavioral therapy can be provided before or after administration within a period of 1, 2, 3, 4, 5, 6, 7, 8, 9 or 10 half-lives of the compound administered.

[0451] It will be understood that the disclosed co-therapeutic methods can be used in conjunction with the compounds, compositions, kits and uses of the present invention.

[0452] e. Combination therapy

[0453] In the methods of use described herein, one or more additional therapeutic agents can be administered simultaneously or sequentially with the compounds and compositions of the present invention. Sequential administration includes administration before or after the compounds and compositions of the present invention. In some embodiments, one or more additional therapeutic agents can be administered in the same composition as the compounds of the present invention. In other embodiments, there may be a time interval between the administration of additional therapeutic agents and the compounds of the present invention. In some embodiments, the administration of additional therapeutic agents together with the disclosed compounds can allow for lower doses of other therapeutic agents and / or administration at lower frequency intervals. When used in combination with one or more other active ingredients, the compounds of the present invention and other active ingredients can be used at lower doses than when used alone. Therefore, the pharmaceutical compositions of the present invention include pharmaceutical compositions containing one or more other active ingredients in addition to compounds of formula (I) or pharmaceutically acceptable salts thereof. The above combinations include not only combinations of the compounds of the present invention with one other active compound, but also combinations of the compounds of the present invention with two or more other active compounds.

[0454] The compounds of the present invention can be used as a single agent, or in combination with one or more other drugs for the treatment, prevention, control, improvement of the aforementioned diseases, disorders and conditions (for which the compound or other drugs have utility) or reduction of their risks, wherein the drug combination is safer or more effective than the use of any one drug alone. One or more other drugs can be administered simultaneously or sequentially with the disclosed compound by a route and its commonly used amount. When the compounds of the present invention are used simultaneously with one or more other drugs, a pharmaceutical composition in a unit dosage form containing such drugs and the compounds of the present invention can be used. However, combination therapy can also be administered with an overlapping schedule. It is also envisioned that the combination of one or more active ingredients with the compounds of the present invention can be more effective than any one as a single agent. Therefore, when used in combination with one or more other active ingredients, the compounds of the present invention and other active ingredients can be used at a lower dose than when each is used alone.

[0455] The pharmaceutical compositions and methods of the present invention may further comprise other therapeutically active compounds mentioned herein, which are generally used to treat the above-mentioned pathological symptoms.

[0456] The combination above not only includes the combination of the disclosed compound and a kind of other active compounds, but also includes the combination with two or more other active compounds. Similarly, the compound of the present invention can be used in combination with other drugs for preventing, treating, controlling, improving the disease or illness useful to the compound of the present invention or reducing the risk of these diseases or illness. Such other drugs can be used simultaneously or sequentially with the compound of the present invention by a kind of approach and its commonly used amount. Therefore, in an embodiment, in addition to the compound of the present invention, the pharmaceutical composition also contains one or more other active ingredients.

[0457] The weight ratio of the disclosed compound to the second active ingredient can vary and will depend on the effective dose of each ingredient. Typically, each effective dose will be used. Thus, for example, when the compounds of the present invention are combined with another agent, the weight ratio of the disclosed compound to the other agent will typically be in the range of from 200:1 to about 1:200. Compositions of the compounds of the present invention with other active ingredients will also typically be within the above ranges, but in each case, an effective dose of each active ingredient should be used.

[0458] In such combinations, the compounds of the present invention and other active agents may be administered alone or in combination. In addition, the administration of one element may be performed before, simultaneously with, or subsequently to the administration of one or more other agents.

[0459] Therefore, the compounds of the present invention can be used alone or in combination with other medicaments known to be beneficial in the subject's indications or other drugs that affect receptors or enzymes, which can increase the efficacy, safety, convenience or reduce the unwanted side effects of the compounds of the present invention. The compounds of the present invention and other medicaments can be used together in concomitant therapy or in a fixed combination.

[0460] In some embodiments, the compounds can be used in combination with anti-Alzheimer's disease agents, β-secretase inhibitors, cholinergic agents, γ-secretase inhibitors, HMG-CoA reductase inhibitors, M1 allosteric agonists, M1 positive allosteric modulators, NSAIDs (including ibuprofen), vitamin E, and anti-amyloid antibodies. In another embodiment, the subject compounds can be used in combination with sedatives, hypnotics, anxiolytics, antipsychotics (typical and atypical), anxiolytics, cyclopyrrolone, imidazopyridine, pyrazolopyrimidine, weak tranquilizers, melatonin agonists and antagonists, melatonergic agents, benzodiazepines, barbiturates, 5HT2 antagonists, etc., such as: adizalam, allobarbital, allomidone, alprazolam, amisulpride, amitriptyline, amobarbital, amoxapine, aripiprazole, benzazepam, benzylpezil Zolpidem, brotizolam, bupropion, buspirone, butabarbital, butalbital, epiprazole, carprourea, carbochloral, chloral betaine, chloral hydrate, clomipramine, clonazepam, chlorpiperazine, clorazepam, chlordiazepoxide, chlorpromazine, clozapine, ciproazepam, desipramine, declamol, diazepam, chloralpyrine, divalproex, diphenhydramine, donepezil, memantine, galantamine, doxepin, estazolam, ethchlorvinox, etomidate, fenobant, flunitrazepam, zofopentixol , flupentixol, fluphenazine, flurazepam, fluvoxamine, fluoxetine, fulrazepam, phenacetin, halazepam, haloperidol, hydroxyzine, imipramine, lithium, lorazepam, lormetazepam, maprotiline, chloroquine, melatonin, mebendal, meprobamate, methaqualone, midaflutol, midazolam, nefazodone, nisolamide, nitrazepam, nortriptyline, olanzapine, oxazepam, paroxetine, pentobarbital, perampapine, perphenazine, phenelzine, phenobarbital, prazepam, promethazine, propofol, Protriptyline, quazepam, quetiapine, resclozepam, risperidone, roletomide, secobarbital, sertraline, suprolone, temazepam, thioridazine, thiothixene, tracazolate, tranylcypromine, trazodone, triazolam, tripipam, trimethoprim-butyl acetate, triclofossil, trifluoperazine, trimetozin, trimipramine, udazepam, zaleplon, ziprasidone, zolazepam, zolpidem, and salts thereof, and combinations thereof, etc. Alternatively, the subject compound can be administered in combination with a physical method (e.g., phototherapy or electrical stimulation).

[0461] In some embodiments, the compounds of the invention may be used in combination with levodopa (with or without a selective extracerebral decarboxylase inhibitor such as carbidopa or benserazide), anticholinergics (such as biperiden (optionally as its hydrochloride or lactate) and trihexyphenidyl (benhexyphenidyl) hydrochloride), COMT inhibitors (such as entacapone), MOA-B inhibitors (such as rasagiline), antioxidants, A2a adenosine receptor antagonists (such as istradefylline), cholinergic agonists, NMDA receptor antagonists (such as ketamine), serotonin receptor antagonists and dopamine receptor agonists (such as alendronate, bromocriptine, fenoldopam, lisuride, nagolide, pergolide and pramipexole). It will be appreciated that the dopamine agonist may be in the form of a pharmaceutically acceptable salt, for example, alentimod hydrobromide, bromocriptine mesylate, fenoldopam mesylate, nagolide hydrochloride and pergolide mesylate. Lisuride and pramipexole are usually used in non-salt form.

[0462] In certain embodiments, the compounds of the present invention can be used in combination with compounds from the class of phenothiazines, thioxanthenes, heterocyclic dibenzazepines, butyrophenones, diphenylbutylpiperidines and indolones neuroleptics. Suitable examples of phenothiazines include chlorpromazine, mesoridazine, thioridazine, acetophenazine, fluphenazine, perphenazine and trifluoperazine. Suitable examples of thioxanthenes include chlorprothixene and aminothiothixene. The example of dibenzazepines is clozapine. The example of butyrophenones is haloperidol. The example of diphenylbutylpiperidines is pimozide. The example of indolones is indolone. Other antipsychotics include loxapine, sulpiride and risperidone. It is understood that when used in combination with the subject compound, the antipsychotic can be in the form of a pharmaceutically acceptable salt, for example, chlorpromazine hydrochloride, mesoridazine besylate, thioridazine hydrochloride, acetophenazine maleate, fluphenazine hydrochloride, fluphenazine enanthate, fluphenazine decanoate, trifluoperazine hydrochloride, aminothiothixene hydrochloride, haloperidol decanoate, loxapine succinate and molinone hydrochloride. Perphenazine, chlorprothixene, clozapine, haloperidol, pimozide and risperidone are usually used in non-salt form. Thus, the subject compounds can be used in combination with acetophenazine, alentimol, amisulpride, trihexyphenidyl, bromocriptine, biperiden, chlorpromazine, chlorprothixene, clozapine, diazepam, fenoldopam, fluphenazine, haloperidol, levodopa, levodopa with benserazide, levodopa with carbidopa, lisuride, loxapine, mesoridazine, molindonal, nagolide, olanzapine, pergolide, perphenazine, pimozide, pramipexole, quetiapine, risperidone, sulpiride, tetrabenazine, deutetrabenazine, trihexyphenidyl, thioridazine, thiothixene, trifluoperazine, or ziprasidone.

[0463] In some embodiments, the compounds of the present invention can be used in combination with antidepressants or antianxiety agents, including norepinephrine reuptake inhibitors (including tertiary amine tricyclics and secondary amine tricyclics), selective serotonin reuptake inhibitors (SSRIs), monoamine oxidase inhibitors (MAOIs), reversible inhibitors of monoamine oxidase (RIMAs), serotonin and norepinephrine reuptake inhibitors (SNRIs), corticotropin releasing factor (CRF) antagonists, α-adrenergic receptor antagonists, neurokinin-1 receptor antagonists, atypical antidepressants, benzodiazepines, 5-HT 1A Agonists or antagonists (especially 5-HT1A partial agonists), and corticotropin-releasing factor (CRF) antagonists. Specific agents include: amitriptyline, clomipramine, doxepin, imipramine and trimipramine; amoxapine, desipramine, maprotiline, nortriptyline and protriptyline; fluoxetine, vortioxetine, fluvoxamine, paroxetine and sertraline; isocarboxazid, phenelzine, escitalopram, tranylcypromine and selegiline; moclobemide: venlafaxine; duloxetine; aprepitant; bupropion, lithium, nefazodone, trazodone and viloxazine; alprazolam, chlordiazepoxide, clonazepam, chlordiazepoxide dipotassium, diazepam, halazepam, lorazepam, oxazepam and prazepam; buspirone, flecinoxan, gepirone, and ixabepilone, and pharmaceutically acceptable salts thereof.

[0464] In some embodiments, the compounds of the present invention can be co-administered with an orthosteric muscarinic agonist, a muscarinic potentiator, or a cholinesterase inhibitor. In some embodiments, the compounds can be co-administered with a GlyT1 inhibitor, such as, but not limited to, risperidone, quetiapine, clozapine, haloperidol, fluoxetine, prazepam, xanomeline, lithium, phenobarbital, and salts thereof, and combinations thereof.

[0465] f. Mode of administration

[0466] The method for treatment may include any number of ways of administering the compound or composition of the present invention. The mode of administration may include tablets, pills, dragees, hard and soft gel capsules, granules, pellets, aqueous, lipid, oily or other solutions, emulsions (e.g., oil-in-water emulsions), liposomes, aqueous or oily suspensions, syrups, elixirs, solid emulsions, solid dispersions or dispersible powders. In order to prepare a pharmaceutical composition for oral administration, the medicament may be mixed with adjuvants and excipients commonly known and used, such as gum arabic, talcum powder, starch, sugars (e.g., mannose, methylcellulose, lactose), gelatin, surfactants, magnesium stearate, aqueous or non-aqueous solvents, paraffin derivatives, cross-linking agents, dispersants, emulsifiers, lubricants, preservatives, flavorings (e.g., ether oils), solubility enhancers (e.g., benzyl benzoate or benzyl alcohol) or bioavailability enhancers (e.g., Gelucire.TM.). In pharmaceutical compositions, the agent may also be dispersed in microparticles (eg, nanoparticle compositions).

[0467] For parenteral administration, the agent can be dissolved or suspended in a physiologically acceptable diluent, such as water, a buffer, an oil with or without a solubilizing agent, a surfactant, a dispersant or an emulsifier. As oils, for example, but not limited to, olive oil, peanut oil, cottonseed oil, soybean oil, castor oil and sesame oil can be used. More generally, for parenteral administration, the agent can be in the form of an aqueous, lipid, oily or other type of solution or suspension, or even administered in the form of a liposome or nanosuspension.

[0468] As used herein, the term "parenteral" refers to modes of administration including intravenous, intramuscular, intraperitoneal, intrasternal, subcutaneous, and intraarticular injection and infusion.

[0469] The compounds and methods of the present invention will be better understood by reference to the following examples, which are intended to illustrate but not to limit the scope of the invention.

[0470] 5. Examples

[0471] All NMR spectra were recorded on a 400 MHz AMX Bruker NMR spectrometer, a 400 MHz Bruker Avance AV-III-400 spectrometer or a Varian MR400 spectrometer. 1H chemical shifts are reported as δ values ​​(in ppm) relative to the residual protic solvent as an internal standard. Data are reported as follows: chemical shift, multiplicity (s = singlet, bs = broad singlet, d = doublet, t = triplet, q = quartet, dd = doublet, m = multiplet, ABq = AB quartet), coupling constant, integration. Reverse phase LCMS analysis was performed using an Agilent 1200 system consisting of a binary pump with degasser, high performance autosampler, thermostatic column compartment, C18 column, diode array detector (DAD), and Agilent 6150 MSD using the following parameters. Gradient conditions were 5% to 95% acetonitrile in 1.4 minutes with the aqueous phase being 0.1% TFA in water, holding at 95% acetonitrile for 0.1 min, 0.5 mL / min, 55° C. (“90 sec method”). The sample was separated at 0.5 mL / min on a Waters Acquity UPLC BEH C18 column (1.7 μm, 1.0 × 50 mm), with column and solvent temperatures maintained at 55 ° C. DAD was set to scan from 190 nm to 300 nm, and the signals used were 220 nm and 254 nm (both bandwidths were 4 nm). The MS detector was configured with an electrospray ionization source, and a low-resolution mass spectrum was obtained by scanning from 140 to 700 AMU (step length of 0.2 AMU, at 0.13 cycles / second, peak width of 0.008 minutes). The dry gas flow was set to 13 liters per minute at 300 ° C, and the nebulizer pressure was set to 30 psi. The capillary needle voltage was set to 3000 V, and the fragmentation voltage was set to 100 V. Data acquisition was performed using Agilent Chemstation and Analytical Studio Reviewer software.

[0472] The abbreviations used in the following examples and reaction schemes include the following: aq is aqueous solution; Boc is tert-butyloxycarbonyl; BrettPhos is 2-(dicyclohexylphosphino)3,6-dimethoxy-2′,4′,6′-triisopropyl-1,1′-biphenyl; BrettPhos Pd G3 is [(2-di-cyclohexylphosphino-3,6-dimethoxy-2′,4′,6′-triisopropyl-1,1′-biphenyl)-2-(2′-amino-1,1′-biphenyl)]methanesulfonate palladium(II); t-BuOH is tert-butyl alcohol; DCE is 1,2-dichloroethane; DCM is dichloromethane; DEA is diethylamine; DMF is N,N-dimethylformamide; DMP or Dess-Martin periodinane is 1,1,1-tri(acetoxy)-1,1-dihydro-1,2-benzidoyl-3-(1H)-one; DIAD is diisopropyl azodicarboxylate; DIPEA or DIEA is diisopropylethylamine; DMAP is 4-dimethylaminopyridine; DMSO is dimethyl sulfoxide; Dowtherm A is a eutectic mixture of 26.5% diphenyl + 73.5% diphenyl oxide; DtBAD is di-tert-butyl azodicarboxylate; eq or eq. is equivalent; EtOAC is ethyl acetate; EtOH is ethanol; h is hour; Hex is hexane; IPA is isopropanol; KOAc is potassium acetate; LAH is lithium aluminum hydride; mCPBA is meta-chloroperbenzoic acid; MeCN or ACN is acetonitrile; MeOH is methanol; min is minute; NaOAc is Sodium acetate; NaOMe is sodium methoxide; NCS is N-chlorosuccinimide; NMP is N-methyl-2-pyrrolidone; Pd(dppf)Cl2 is [1,1′-bis(diphenylphosphino)ferrocene]dichloropalladium(II); Pd(OAc)2 is palladium(II) acetate; PPA is polyphosphoric acid; PPh3 is triphenylphosphine; PPTS is pyridinium p-toluenesulfonate; rt is room temperature; sat. is saturated; sec is seconds; SCX column or HF SCX column is a strong cation exchange column (i.e., Agilent part number 14256027); SFC is supercritical fluid chromatography; TBAC or TBACl is tetrabutylammonium chloride; t-BuXPhos is 2-di-tert-butylphosphino-2′,4′,6′-triisopropylbiphenyl; TEA is triethylamine; TFA is trifluoroacetic acid; THF is tetrahydrofuran; TosCl is p-toluenesulfonyl chloride; and tosyl is p-toluenesulfonyl.

[0473] a. Preparation of intermediates

[0474]

[0475] 5-(((6-chloro-5-methylpyridazin-3-yl)amino)methylene)-2,2-dimethyl-1,3-dioxane-4,6-dione (Intermediate A): To a solution of 6-chloro-5-methylpyridazin-3-amine (1 g) in ethanol (12 mL) was added triethyl orthoformate (1.16 mL) and isopropylidene malonate (1.04 g) at ambient temperature. The mixture was then heated to 60 °C for 18 hours. The mixture was filtered and the filter cake was washed with ethanol (5 mL x 3) to give the intermediate 5-[[(6-chloro-5-methyl-pyridazin-3-yl)amino]methylene]-2,2-dimethyl-1,3-dioxane-4,6-dione (1.59 g, 77% yield). ES-MS [M+1] + :298.2

[0476] 7-Chloro-8-methyl-4H-pyrimido[1,2-b]pyridazin-4-one (Intermediate B): A solution of 5-[[(6-chloro-5-methyl-pyridazin-3-yl)amino]methylene]-2,2-dimethyl-1,3-dioxane-4,6-dione (1.59 g) in Dowtherm A (8 mL) was stirred at 220 ° C for 1 hour. After cooling, the mixture was added to water and acidified with 1M HCl. The aqueous layer was extracted with hexane (3x125 mL) to remove Dowtherm A. The aqueous layer was neutralized with NaHCO3 aqueous solution, and the mixture was extracted with chloroform / IPA (4:1) (3x). The combined organic layers were dried over magnesium sulfate and concentrated to give the title compound (908 mg, 67% yield). 1 H NMR (400MHz, DMSO) δ 8.23 ​​(d, J = 6.5 Hz, 1H), 8.03 ( q, J = 1.3 Hz, 1H), 6.54 ( d, J = 6.4 Hz, 1H), 2.44 ( d, J = 1.3 Hz, 3H). ES-MS[M+1] + :196.

[0477]

[0478] 7- Chloro-8,9-dimethyl-pyrimido[1,2-b]pyridazine-4-one (Intermediate C): A mixture of 6- chloro-3-amino-4,5-dimethylpyridazine (550 mg), ethyl 3,3-diethoxypropionate (1.02 mL) and polyphosphoric acid (5 mL) was added to a sealed tube. The mixture was stirred at 85 ° C for 5 hours and then cooled to room temperature, wherein additional ethyl 3,3-diethoxypropionate (1.02 mL) was added to the reaction mixture. The vial was sealed and the mixture was heated to 85 ° C for 18 hours. The reaction mixture was then slowly added to a stirring solution of 150 mL saturated NaHCO3 aqueous solution and 100 mL of DCM. After complete addition, the mixture was allowed to stir for 20 minutes, the organic layer was separated, and the aqueous layer was further extracted with chloroform / IPA (4: 1) (x3). The organic layer was collected, dried over MgSO4, filtered and concentrated. The crude product was purified using a Teledyne ISCO Combi-Flash system (liquid load with DCM, 40G column, 0%-70% EtOAc / Hex, 35 min run) to give the title compound (366 mg, 50% yield) as a solid. 1 H NMR (400MHz, CDCl3) δ 8.22-8.20 (d, J = 6.5Hz, 1H), 6.64-6.62 (d, J = 6.5Hz, 1H), 2.62 (d, J = 0.6Hz, 3H), 2.51 (d, J = 0.6Hz, 3H). ES-MS[M+1] + :210.

[0479]

[0480] 7- Chloro-2,8-dimethyl-4H-pyrimido[1,2-b]pyridazine-4-one (Intermediate D): Ethyl acetoacetate (1.76 mL) was added to a solution of 6- chloro-3-amino-5-methylpyridazine (1 g) in polyphosphoric acid (5 mL). The mixture was heated to 120 ° C for 18 hours. The mixture was slowly added to a stirring solution of 150 mL saturated NaHCO3 while hot. The aqueous layer was extracted three times with chloroform: IPA (4: 1). The organic layer was dried over magnesium sulfate, filtered and concentrated under vacuum. The crude product was purified using a Teledyne ISCO Combi-Flash system (liquid load containing DCM, 80G column, 0%-70% EtOAc / DCM, running 42 min). The fractions containing the desired product were concentrated to give the title compound (1.34 g, 92% yield) as a solid. 1H NMR (400MHz, CDCl3) δ7.59 (q, J=1.3Hz, 1H), 6.50 (s, 1H), 2.50 (d, J=1.3Hz, 3H), 2.43 (d, J=0.7Hz, 3H). ES-MS[M+1] + :210.

[0481]

[0482] 7- Chloro-2,3,8- trimethyl -4H- pyrimido [1,2-b] pyridazine -4- ketone (Intermediate E): 6- chloro-3-amino-5-methyl pyridazine (500mg) in Dowtherm A (2mL) solution was added 2- methyl -3- oxo -butyric acid ethyl ester (591μL), and the reaction was heated to 150 ° C for 18 hours. Additional 2- methyl -3- oxo -butyric acid ethyl ester (591μL) was added, and the reaction was microwaved at 180 ° C for 15 minutes. The solution was directly loaded onto a Teledyne ISCO Combi-Flash system (120G column, 100% Hex, 6min; then 0% -80% EtOAc / DCM, run 15min) to obtain the title compound (442mg, 57% yield) as a solid. 1 H NMR (400MHz, CDCl3) δ7.53 (q, J=1.3Hz, 1H), 2.47 (d, J=1.3Hz, 3H), 2.45 (s, 3H), 2.26 (s, 3H). ES-MS[M+1] + :224.

[0483]

[0484] 7- Chloro-2,8,9-trimethyl-4H-pyrimido[1,2-b]pyridazine-4-one (Intermediate F): Ethyl acetoacetate (803 μL) was added to a solution of 6- chloro-3-amino-4,5-dimethylpyridazine (500 mg) in polyphosphoric acid (4.5 mL). The mixture was heated to 120 ° C for 1 hour. The mixture was slowly added to 125 mL of saturated NaHCO3 and 50 mL of chloroform / IPA (4: 1) while hot. After the addition was completed, the mixture was allowed to stir for 15 minutes and the organic layer was separated. The aqueous layer was further extracted with chloroform: IPA (4: 1) (x3). The organic layer was dried over magnesium sulfate, filtered and concentrated under vacuum. The crude product was dissolved in 5 mL of MeOH, sonicated, and the precipitate was collected by vacuum filtration and washed with hexane. The solid was dried under nitrogen to obtain the title compound (472 mg, 67% yield) as a solid. 1H NMR (400MHz, CDCl3) δ6.49 (s, 1H), 2.60 (d, J = 0.8Hz, 3H), 2.48 (q, J = 0.7Hz, 3H), 2.44 (q, J = 0.5Hz, 3H). ES-MS[M+1] + :224.

[0485]

[0486] 7-Chloro-8-methyl-2-(trifluoromethyl)-4H-pyrimido[1,2-b]pyridazin-4-one (Intermediate G): To a solution of 6-chloro-3-amino-5-methylpyridazine (500 mg) in polyphosphoric acid (5 mL) was added ethyl 4,4,4-trifluoroacetoacetate (1.02 mL). The mixture was heated to 120 °C for 18 hours. While hot, the mixture was slowly added to a beaker containing 125 mL of saturated NaHCO3 and 50 mL of chloroform / IPA (4:1) while maintaining the pH at about 7. After the addition was complete, the mixture was allowed to stir for 15 minutes and the organic layer was separated. The aqueous layer was further extracted three times with chloroform:IPA (4:1). The organic layer was dried over magnesium sulfate, filtered and concentrated under vacuum. The crude product was dissolved in 15 mL of DMSO (any solids that precipitated were collected to give the pure desired product), and the filtrate was purified by reverse phase chromatography using a Gilson HPLC (50×250 mm column, 5%-45% ACN / 0.05% NH 4 OH aqueous solution, run 16 min). The fractions containing the product were concentrated and combined with the previous solid to give the title compound (393 mg, 43% yield) as a solid. 1 H NMR (400MHz, CDCl3) δ7.79 (q, J=1.3Hz, 1H), 6.98 (s, 1H), 2.56 (d, J=1.3Hz, 3H). ES-MS[M+1] + :264.

[0487]

[0488] 7-Chloro-8,9-dimethyl-2-(trifluoromethyl)-4H-pyrimido[1,2-b]pyridazin-4-one (Intermediate H): To a solution of 6-chloro-3-amino-4,5-dimethylpyridazine (550 mg) in polyphosphoric acid (5 mL) was added ethyl 4,4,4-trifluoroacetoacetate (1.02 mL). The mixture was heated to 120° C. for 18 hours. Additional ethyl 4,4,4-trifluoroacetoacetate (1.02 mL) was added and the mixture was stirred at 120° C. for another 7 hours. While hot, the mixture was slowly added to a beaker containing 125 mL of saturated NaHCO3 and 50 mL of chloroform / IPA (4:1) while maintaining the pH at about 7. After the addition was complete, the mixture was allowed to stir for 15 minutes and the organic layer was separated. The aqueous layer was further extracted three times with chloroform:IPA (4:1). The organic layer was dried over magnesium sulfate, filtered and concentrated under vacuum. The crude product was dissolved in DMSO (16 mL) and purified by reverse phase chromatography using a Gilson HPLC (50×250 mm column, 5%-50% ACN / 0.05% NH 4 OH in water, 16 min run). Product containing fractions were concentrated to give the title compound (264 mg, 27% yield) as a solid. 1 H NMR (400MHz, CDCl3) δ6.96 (s, 1H), 2.57 (q, J = 0.9Hz, 3H), 2.48 (q, J = 0.9Hz, 3H). ES-MS[M+1] + :278.

[0489]

[0490] 7-Chloro-2-(difluoromethyl)-8-methyl-4H-pyrimido[1,2-b]pyridazin-4-one (Intermediate I): To a solution of 6-chloro-3-amino-5-methylpyridazine (500 mg) in polyphosphoric acid (5 mL) was added ethyl 4,4-difluoroacetoacetate (912 μL). The mixture was heated to 120 °C for 2 hours. While hot, the mixture was slowly added to a beaker containing 125 mL of saturated NaHCO3 and 50 mL of chloroform / IPA (4:1) while maintaining the pH at about 7. After the addition was complete, the mixture was allowed to stir for 15 minutes and the organic layer was separated. The aqueous layer was further extracted three times with chloroform:IPA (4:1). The organic layer was dried over magnesium sulfate, filtered and concentrated under vacuum. The crude product was dissolved in DMSO (15 mL) and purified by reverse phase chromatography using a Gilson HPLC (50×250 mm column, 0%-45% ACN / 0.05% NH 4 OH in water, 16 min run). Product containing fractions were concentrated to give the title compound (640 mg, 75% yield) as a solid. 1H NMR (400MHz, CDCl3) δ7.73 (q, J=1.3Hz, 1H), 6.90 (s, 1H), 6.46 (t, J=54.8Hz, 1H), 2.55 (d, J=1.3Hz, 3H). ES-MS[M+1] + :246.

[0491]

[0492] 7-Chloro-2-(difluoromethyl)-8,9-dimethyl-4H-pyrimido[1,2-b]pyridazine-4-one (Intermediate J): To a solution of 6-chloro-3-amino-4,5-dimethylpyridazine (1.65 g) in polyphosphoric acid (10 mL) was added ethyl 4,4-difluoroacetoacetate (2.74 mL). The mixture was heated to 120 ° C for 6 hours. The reaction mixture was then slowly added to a stirred saturated NaHCO solution (200 mL) while hot. Once the pH was about 7, the aqueous layer was extracted with chloroform: IPA (4: 1). The organic layer was dried over magnesium sulfate, filtered and concentrated under vacuum. The crude product was purified using a Teledyne ISCO Combi-Flash system (liquid load containing DCM, 120G column, 0%-40% EtOAc / DCM, run 15 min) to give the title compound as a solid (1.05 g, 39% yield). 1 H NMR (400MHz, CDCl3) δ6.90 (s, 1H), 6.48 (t, J = 54.9Hz, 1H), 2.64 (s, 3H), 2.53 (s, 3H). ES-MS[M+1] + :260.

[0493]

[0494] 7- Chloro-2-cyclopropyl-8-methyl-4H-pyrimido[1,2-b]pyridazine-4-one (Intermediate K): A solution of 6- chloro-3-amino-5-methylpyridazine (500 mg) and ethyl 3-cyclopropyl-3-oxopropanoate (617 μL) in DOWTHERM A (5 mL) was added to a sealed vial. The vial was sealed and the reaction was heated to 150 ° C for 18 h. Additional ethyl 3-cyclopropyl-3-oxopropanoate (617 μL) was added and the reaction was heated to 220 ° C for 3 h. The material was directly loaded onto a Teledyne ISCO Combi-Flash system (liquid load, 120G column, 100% Hex, run 6 min; then 0%-40% EtOAc / DCM, run 15 min) to obtain the title compound as a solid (44 mg; 5% yield). 1H NMR (400MHz, CDCl3) δ7.52 (q, J = 1.3Hz, 1H), 6.51 (s, 1H), 2.47 (d, J = 1.4Hz, 3H), 1.97-1.86 (m, 1H), 1.18-1.10 (m, 2H), 1.08-0.97 (m, 2H). ES-MS[M+1] + :236.

[0495]

[0496] 7-Chloro-2-ethyl-8,9-dimethyl-4H-pyrimido[1,2-b]pyridazine-4-one (Intermediate L): To a solution of 6-chloro-3-amino-4,5-dimethylpyridazine (550 mg) in polyphosphoric acid (4 mL) was added ethyl 3-oxopentanoate (913 μL). The mixture was heated to 85 °C for 1 hour and then heated to 120 °C for 1 hour. While hot, the mixture was slowly transferred to a stirred solution of saturated NaHCO3 aqueous solution (about 150 mL) and chloroform / IPA (4:1) (50 mL). After the addition was complete, the mixture was stirred for 10 minutes and then the organic layer was separated. The aqueous layer was further extracted with chloroform / IPA (4:1) (x3), and the organic layers were pooled, dried over MgSO4, filtered, and concentrated under vacuum. After cooling, a solid precipitate was observed. Hexanes were added, the suspension was sonicated, and the solid was collected by vacuum filtration and washed with hexanes to afford the title compound (469 mg, 57% yield) in >95% purity by LCMS. 1 H NMR (400MHz, CDCl3) δ6.50 (s, 1H), 2.70 (q, J = 7.6Hz, 2H), 2.61 (s, 3H), 2.48 (s, 3H), 1.30 (t, J = 7.6Hz, 3H). ES-MS[M+1] + :238.

[0497]

[0498] 7-Chloro-2-isopropyl-8,9-dimethyl-4H-pyrimido[1,2-b]pyridazine-4-one (Intermediate M): To a solution of 6-chloro-3-amino-4,5-dimethylpyridazine (550 mg) in polyphosphoric acid (5 mL) was added 4-methyl-3-oxo-pentanoic acid ethyl ester (1.13 mL). The mixture was heated to 85 ° C for 1 hour and then heated to 120 ° C for 1 hour. The mixture was slowly added to a stirred solution of saturated NaHCO3 aqueous solution (150 mL) and chloroform / IPA (4: 1) (50 mL) while hot. After the addition was completed, the mixture was stirred for 10 minutes and the organic layer was separated. The aqueous layer was further extracted with chloroform / IPA (4: 1) (3x50 mL), and the organic layers were collected, dried over MgSO4, filtered, and concentrated. The crude product was purified using a Teledyne ISCO Combi-Flash system (liquid load with DCM, 80G column, 0%-65% EtOAc / Hex, 20 min run). The column was then flushed with hexanes followed by 0%-2% MeOH / DCM for 10 min to give the title compound as a solid (204 mg; 23% yield). 1 H NMR (400MHz, CDCl3) δ6.47 (s, 1H), 3.63 (hept, J = 6.8Hz, 1H), 2.56 (d, J = 1.0Hz, 3H), 2.44 (d, J = 1.0Hz, 3H), 1.32 (s, 3H), 1.30 (s, 3H). ES-MS[M+1] + :252.

[0499]

[0500] 7-Chloro-2-(methoxymethyl)-8,9-dimethyl-4H-pyrimido[1,2-b]pyridazine-4-one (Intermediate N): 4-methoxy-3-oxo-butyric acid methyl ester (1.23 mL) was added to a solution of 6-chloro-3-amino-4,5-dimethylpyridazine (1 g) in polyphosphoric acid (9 mL). The mixture was heated to 85 ° C for 1 hour and then heated to 120 ° C for 30 minutes. The mixture was then quenched by slowly adding it to a solution of saturated sodium bicarbonate (about 150 mL) and 4: 1 chloroform / IPA (about 50 mL). The organic layer was separated and the aqueous layer was further extracted (x3). The organic layers were collected, dried over magnesium sulfate, filtered and concentrated. The crude product was dissolved in DMSO (9 mL) and the solid was filtered out. The filtrate was purified using Gilson (basic, 50×250 mm column, 5%-55% ACN / 0.05% NH4OH aqueous solution, running 16 min). The fractions containing the product were concentrated to give the title compound (219 mg) as a solid. The solid was washed with hexane, then dissolved in DCM and purified using a Teledyne ISCO Combi-Flash system (liquid load containing DCM, 80G column, 0%-2% MeOH / DCM / NH4OH, running 10 min). The fractions containing the product were concentrated to give the title compound (682 mg; 43% yield) as a solid. 1 H NMR (400MHz, CDCl3) δ6.77 (t, J = 1.0 Hz, 1H), 4.45 (d, J = 1.0 Hz, 2H), 3.51 (s, 3H), 2.59 (d, J = 0.9 Hz, 3H), 2.49 (d, J = 1.0 Hz, 3H). ES-MS[M+1] + :254.

[0501]

[0502] 7-Chloro-2-(methoxymethyl)-8-methyl-4H-pyrimido[1,2-b]pyridazine-4-one (Intermediate O): 4-methoxy-3-oxo-butyric acid methyl ester (673 μL, 5.22 mmol) was added to a solution of 6-chloro-3-amino-5-methylpyridazine (500 mg) in polyphosphoric acid (5 mL). The mixture was heated to 85 ° C for 1 hour and then heated to 120 ° C for 30 minutes. The mixture was then quenched by slowly adding it to a solution of saturated sodium bicarbonate (about 150 mL) and 4: 1 chloroform / IPA (about 50 mL). The organic layer was separated and the aqueous layer was further extracted (x3). The organic layers were collected, dried over magnesium sulfate, filtered and concentrated. The crude product was dissolved in DMSO (12 mL) and purified by reverse phase chromatography using a Gilson HPLC (50×250 mm column, 0%-45% ACN / 0.05% NH 4 OH in water, 16 min run). Product containing fractions were concentrated to give the title compound as a solid (690 mg, 83% yield). 1 H NMR (400MHz, CDCl3) δ7.61 (q, J = 1.3Hz, 1H), 6.76 (d, J = 1.1Hz, 1H), 4.42 (s, 2H), 3.50 (s, 3H), 2.50 (d, J = 1.2Hz, 3H). ES-MS[M+1] + :240.

[0503]

[0504] 7-Chloro-8-methyl-4-oxo-4H-pyrimido[1,2-b]pyridazine-2-carboxylic acid ethyl ester (Intermediate P): A mixture of diethyl ethoxymethylenemalonate (641 μL) and 6-chloro-3-amino-4,5-dimethylpyridazine (500 mg) in Dowtherm A (6 mL) was heated to 200 ° C for 18 hours. The reaction mixture was cooled to room temperature and loaded directly onto a Teledyne ISCO Combi-Flash system (liquid load, 80G column, 0%-55% EtOAc / DCM, running 25 min). The fractions containing the desired product were concentrated and determined to be impure by LCMS. The material was dissolved in DMSO (9 mL) and purified by reverse phase chromatography using a Gilson HPLC (50×250 mm column, 5%-50% ACN / 0.05% NH4OH aqueous solution, running 16 min). The product containing fractions were concentrated to give the title compound as a solid (250 mg; 28% yield). 1H NMR (400MHz, CDCl3) δ8.93 (s, 1H), 7.75 (s, 1H), 4.42 (q, J = 7.1Hz, 2H), 2.57 (d, J = 0.9Hz, 3H), 1.41 (t, J = 7.1Hz, 3H). ES-MS[M+1] + :268.2.

[0505]

[0506] 7-Chloro-3-fluoro-2,8-dimethyl-4H-pyrimido[1,2-b]pyridazine-4-one (Intermediate Q): To a solution of 6-chloro-3-amino-5-methylpyridazine (550 mg) in polyphosphoric acid (5.5 mL) was added ethyl 2-fluoroacetoacetate (961 μL). The mixture was heated to 120 °C for 2 hours. It was then quenched by slowly adding the mixture to a solution of saturated sodium bicarbonate (about 150 mL) and 4:1 chloroform / IPA (about 50 mL). The organic layer was separated and the aqueous layer was further extracted (x3). The organic layers were pooled, dried over magnesium sulfate, filtered and concentrated. The mixture was then quenched by slowly adding it to a solution of saturated sodium bicarbonate (about 150 mL) and 4:1 chloroform / IPA (about 50 mL). The organic layers were separated and the aqueous layer was further extracted (x3). The organic layers were pooled, dried over magnesium sulfate, filtered and concentrated. The crude product was dissolved in DMSO (12 mL) and purified by reverse phase chromatography using a Gilson HPLC (50×250 mm column, 0%-45% ACN / 0.05% NH 4 OH in water, 16 min run). Product-containing fractions were concentrated to give the title compound as a solid (627 mg; 72% yield). 1 H NMR (400MHz, CDCl3) δ7.60 (q, J=1.3Hz, 1H), 2.52-2.47 (m, 6H). ES-MS[M+H] + =228.

[0507]

[0508] 7-Chloro-3-fluoro-2,8,9-trimethyl-4H-pyrimido[1,2-b]pyridazin-4-one (Intermediate R): To a solution of 6-chloro-3-amino-4,5-dimethylpyridazine (550 mg) in Dowtherm A (4 mL) was added ethyl 2-fluoro-3-oxo-butyrate (525 μL) and the reaction was heated to 150° C. for 18 hours. Additional ethyl 2-fluoro-3-oxo-butyrate (525 μL) was added and the reaction was stirred at 150° C. for another 18 hours. The reaction mixture was loaded directly onto a Teledyne ISCO Combi-Flash system (liquid load with DCM, 120G column, 0%-20% Hex / EtOAc, run 6 min; then 0%-30% EtOAc / DCM, run 10 min) to give the title compound (243 mg; 29% yield). 1 H NMR (400MHz, CDCl3) δ2.62 (d, J = 0.9 Hz, 3H), 2.51 (d, J = 3.6 Hz, 3H), 2.48 (t, J = 0.9 Hz, 3H). ES-MS[M+1] + :268.

[0509]

[0510] 3,7-Dichloro-2,8-dimethyl-4H-pyrimido[1,2-b]pyridazin-4-one (Intermediate S): To a solution of 7-chloro-2,8-dimethyl-pyrimido[1,2-b]pyridazin-4-one (50 mg) in DMF (0.66 mL) was added N-chlorosuccinimide (33 mg). The mixture was stirred at room temperature for 18 h. Additional N-chlorosuccinimide (8 mg) was added and the mixture was stirred for 48 h. The mixture was poured into water and extracted with DCM, passed through a phase separator and concentrated. The crude product was purified using a Teledyne ISCO Combi-Flash system (liquid load with DCM, 12G column, 0%-30% EtOAc / DCM, run 10 min). 1 H NMR (400MHz, CDCl3) δ7.65 (q, J=1.3Hz, 1H), 2.60 (s, 3H), 2.51 (d, J=1.3Hz, 3H). ES-MS[M+1] + :244

[0511]

[0512] 3,6-Dichloro-4-(methoxymethyl)-5-methylpyridazine (Intermediate T): A stirred solution of 3,6-dichloro-4-methylpyridazine (2 g), methoxyacetic acid (2.82 mL) and silver nitrate (1.04 g) in water (100 mL) was heated to 72 °C, where ammonium persulfate (4.2 g) was slowly added in portions. The mixture was heated at 72 °C for 30 minutes and then at 90 °C for 1 h. Additional ammonium persulfate (2.4 g) was added in portions and the reaction was allowed to stir at 90 °C for another 1 h. The mixture was cooled to room temperature and slowly poured into a solution of saturated aqueous bicarbonate solution (approximately 150 mL) and chloroform / IPA (4:1) and stirred for 20 minutes, then extracted (x3). The organic layers were pooled, dried over magnesium sulfate, filtered and concentrated. The crude product was dissolved in DMSO (15 mL) and purified by reverse phase chromatography using a Gilson HPLC (20×250 mm column, 15%-60% ACN / 0.1% TFA in water, run 16 min). The fractions containing the product were basified with saturated NaHCO 3 , extracted with 3:1 chloroform / IPA, dried over magnesium sulfate, filtered, and concentrated to give the title compound (1.6 g; 62% yield). 1 H NMR (400MHz, CDCl3) δ4.64(s,2H),3.44(s,3H),2.52(s,3H). ES-MS[M+1] + :208.

[0513]

[0514] 6-Chloro-5-(methoxymethyl)-4-methylpyridazine-3-amine (Intermediate U1): 3,6-Dichloro-4-(methoxymethyl)-5-methyl-pyridazine (1.59 g) was mixed with ammonium hydroxide (78 mL) solution and heated to 210 ° C in a stainless steel pressure vessel for 24 hours. The reaction was cooled to room temperature, and the reaction mixture was transferred to a round-bottom flask containing DCM / MeOH and concentrated. The crude product was dissolved in DMSO (12 mL) and purified by reverse phase chromatography using a Gilson HPLC (50×250 mm column, 0%-35% ACN / 0.05% NH4OH in water, running 16 min) to give the title compound (364 mg; 26% yield). 1 HNMR(400MHz, CDCl3) δ4.72(s,2H),4.59(s,2H),3.42(s,3H),2.23(s,3H). ES-MS[M+1] + :188.

[0515] 6-Chloro-4-(methoxymethyl)-5-methylpyridazin-3-amine (Intermediate U2): The intermediate was synthesized and isolated as described in the previous step (554 mg; 39% yield). 1 H NMR (400MHz, CDCl3) δ5.27(s,2H),4.52(s,2H),3.38(s,3H),2.36(s,3H). ES-MS[M+1] + :188.

[0516]

[0517] 7-Chloro-8-(methoxymethyl)-9-methyl-4H-pyrimido[1,2-b]pyridazine-4-one (Intermediate V): Polyphosphoric acid (3 mL) was added to a mixture of ethyl 3,3-diethoxypropionate (755 μL) and 6-chloro-5-(methoxymethyl)-4-methyl-pyridazine-3-amine (364 mg). The mixture was stirred at 120 ° C for 5 hours. More ethyl 3,3-diethoxypropionate (755 μL) was added and the mixture was stirred for another hour at 120 ° C. The reaction mixture was then slowly added to a stirring solution of 100 mL saturated NaHCO3 aqueous solution and 50 mL of DCM. After the addition was complete, the mixture was allowed to stir for 20 minutes and the organic layer was separated. The aqueous layer was further extracted with chloroform / IPA (4:1) (x3). The organic layers were combined, dried over MgSO4, filtered and concentrated. The crude product was dissolved in DMSO (12 mL) and purified by reverse phase chromatography using a Gilson HPLC (50×250 mm column, 0%-55% ACN / 0.05% NH 4 OH in water, run 16 min). The product-containing fractions were concentrated to give the title compound as a solid (108 mg; 23% yield). 1 H NMR (400MHz, CDCl3) δ8.23 (d, J = 6.4 Hz, 1H), 6.68 (d, J = 6.4 Hz, 1H), 4.65 (s, 2H), 3.49 (s, 3H), 2.71 (s, 3H). ES-MS[M+1] + :240.

[0518]

[0519] 7-Chloro-9-(methoxymethyl)-8-methyl-4H-pyrimido[1,2-b]pyridazine-4-one (Intermediate W): Polyphosphoric acid (5mL) was added to a mixture of ethyl 3,3-diethoxypropionate (1.15mL) and 6-chloro-5-(methoxymethyl)-4-methyl-pyridazine-3-amine (554mg). The mixture was stirred at 120°C for 1 hour. More ethyl 3,3-diethoxypropionate (1.15mL) was added and the mixture was stirred at 120°C for another hour. The reaction mixture was then slowly added to a stirring solution of 150mL saturated NaHCO3 aqueous solution and 100mL of DCM. After the addition was complete, the mixture was allowed to stir for 20 minutes and the organic layer was separated. The aqueous layer was further extracted with chloroform / IPA (4:1) (x3). The organic layers were combined, dried over MgSO4, filtered and concentrated. The crude product was dissolved in DMSO (12 mL) and purified by reverse phase chromatography using a Gilson HPLC (50 x 250 mm column, 0%-40% ACN / 0.05% NH4OH in water, 16 min run). Product containing fractions were concentrated to give the title compound as a solid. 1 H NMR (400MHz, CDCl3) δ8.22 (d, J = 6.4 Hz, 1H), 6.64 (d, J = 6.4 Hz, 1H), 4.95 (s, 2H), 3.48 (s, 3H), 2.58 (s, 3H). ES-MS[M+1] + :240.

[0520]

[0521] 3,6-Dichloro-4-cyclopropyl-5-methylpyridazine (Intermediate X): A stirred solution of 3,6-dichloro-4-methylpyridazine (2.5 g), cyclopropanecarboxylic acid (3.66 mL) and silver nitrate (1.3 g) in water (125 mL) was heated to 72 ° C, where ammonium persulfate (5.25 g) was slowly added in portions. The mixture was heated at 72 ° C for 20 minutes. Sulfuric acid (1.23 mL) was added and the mixture was heated to 90 ° C for 1 hour. The mixture was cooled to ambient temperature and slowly poured into a solution of saturated aqueous bicarbonate solution (about 150 mL) and chloroform / IPA (4:1) (about 75 mL), and stirred for 20 minutes, then extracted with chloroform / IPA (4:1) (x3). The organic layers were combined, dried over magnesium sulfate, filtered and concentrated. The crude product was purified using a Teledyne ISCO Combi-Flash system (liquid load with DCM, 80G column, 0%-25% EtOAc / Hex, 15 min run) to give the title compound (1.95 g, 63% yield). 1H NMR (400MHz, CDCl3) δ2.54 (d, J = 0.9Hz, 3H), 1.88-1.75 (m, 1H), 1.34-1.19 (m, 2H), 0.81-0.67 (m, 2H). ES-MS[M+1] + :203.

[0522]

[0523] 6-Chloro-4-cyclopropyl-5-methylpyridazine-3-amine (Intermediate Y1) and 6-Chloro-5-cyclopropyl-4-methylpyridazine-3-amine (Intermediate Y2): 3,6-dichloro-4-cyclopropyl-5-methyl-pyridazine (1.0 g) was mixed with ammonium hydroxide (15 mL; 28% NH3 in water) solution and 1,4-dioxane (2 mL). The solution was heated to 210°C for 24 hours (140 psi). The reaction was cooled to room temperature and the reaction mixture was transferred to a round-bottom flask containing DCM / MeOH and concentrated. The crude product was purified using a Teledyne ISCO Combi-Flash system (solid load, 80G column, 0%-90% EtOAc / DCM, run 25 min; then 0%-10% MeOH / DCM / NH4OH, 10 min) to give a mixture of the title compounds. 1 H NMR (400MHz, CDCl3) δ2.46 (d, J = 1.2Hz, 3H), 1.90-1.79 (m, 1H), 1.42-1.29 (m, 2H), 0.86-0.73 (m, 2H). 1 H NMR (400MHz, CDCl3) δ2.60 (d, J = 1.3Hz, 3H), 1.80-1.55 (m, 1H), 1.44-1.32 (m, 2H), 0.81-0.72 (m, 2H). ES-MS[M+1] + :184.

[0524]

[0525] 7-Chloro-9-cyclopropyl-8-methyl-4H-pyrimido[1,2-b]pyridazine-4-one (Intermediate Z1): To a mixture of isomers 6-chloro-5-cyclopropyl-4-methyl-pyridazine-3-amine and 6-chloro-4-cyclopropyl-5-methyl-pyridazine-3-amine (928 mg) was added ethyl 3,3-diethoxypropionate (1.97 mL) and polyphosphoric acid (7 mL). The mixture was stirred at 120 ° C for 1 hour. More ethyl 3,3-diethoxypropionate (1.97 mL) was added and the mixture was stirred at 120 ° C for another 1 hour. The reaction mixture was then slowly added to a stirring solution of 150 mL saturated NaHCO3 aqueous solution and 100 mL of DCM. After the addition was complete, the mixture was allowed to stir for 20 minutes and the organic layer was separated. The aqueous layer was further extracted with chloroform / IPA (4:1) (x3). The organic layers were combined, dried over MgSO4, filtered and concentrated. The crude product was dissolved in DMSO (15 mL) and purified by reverse phase chromatography using a Gilson HPLC (50×250 mm column, 0%-45% ACN / 0.1% TFA aqueous solution, run 20 min). The fractions containing the product were neutralized with saturated NaHCO3 and extracted with 3:1 chloroform / IPA. The organic layer was concentrated to give the title compound (73 mg, 6% yield) as a solid. ES-MS [M+1] + :236.

[0526] 7-Chloro-8-cyclopropyl-9-methyl-4H-pyrimido[1,2-b]pyridazin-4-one (Intermediate Z2): Intermediate Z2 was synthesized and isolated as described in the previous step to give the title compound as a solid (473 mg; 40% yield). ES-MS [M+1] + :236.

[0527]

[0528] 4-Bromo-6-chloro-5-methylpyridazine-3-amine (Intermediate AA): A mixture of 6-chloro-5-methylpyridazine-3-amine (4 g) and NaHCO (4.68 g) was suspended in MeOH (40 mL) and treated with Br (6.68 g). The mixture was stirred at 20 ° C for 4 hours. The mixture was filtered and the filter cake was washed with a mixture of DCM / MeOH (10: 1) (100 mL). The filtrate was concentrated. The residue was diluted with saturated Na2SO3 aqueous solution (50 mL) and then extracted with DCM (100 mL×5). The combined organic layers were dried over Na2SO4 and concentrated to give 4-bromo-6-chloro-5-methylpyridazine-3-amine (6.07 g). 1 H NMR (400MHz, CDCl3) δ5.86 (br s, 2H), 2.52 (s, 3H).

[0529]

[0530] 9-Bromo-7-chloro-8-methyl-4H-pyrimido[1,2-b]pyridazin-4-one (Intermediate AB): A mixture of 4-bromo-6-chloro-5-methylpyridazin-3-amine (6 g) and diethyl 2-(ethoxymethylene)malonate (11.66 g) in PPA (100 mL) was stirred at 120 ° C for 2 hours. The mixture was added to a saturated aqueous Na2CO3 solution while maintaining the pH at about 8 and extracted with EtOAc (50 mL×4). The combined organic layers were washed with brine (20 mL×2), dried over Na2SO4, filtered and concentrated. The residue was purified by CombiFlash (silica gel, 0% to 70% ethyl acetate in petroleum ether) to give 9-bromo-7-chloro-8-methyl-4H-pyrimido[1,2-b]pyridazin-4-one (4.1 g). 1 H NMR (400MHz, CDCl3) δ8.28 (d, J = 6.8 Hz, 1H), 6.68 (d, J = 6.4 Hz, 1H), 2.73 (s, 3H).

[0531]

[0532] tert-Butyl 4-methylsulfonyloxypiperidine-1-carboxylate (Intermediate AC): To a solution of tert-butyl 4-hydroxypiperidine-1-carboxylate (2.5 g) in DCM (40 mL) at 0°C was added triethylamine (2.42 mL) followed by dropwise addition of methanesulfonyl chloride (1.35 mL). The ice bath was removed and the reaction was stirred at room temperature for 18 h. The reaction mixture was diluted with 3:1 CHCl3 / IPA and water. The layers were separated and the aqueous layer was extracted with 3:1 CHCl3 / IPA (2×). The combined organic layers were washed with brine, dried over MgSO4, filtered, and concentrated to give the title compound 3.4 g. 1 H NMR (400MHz, CDCl3) δ4.91-4.85(m,1H),3.73-3.67(m,2H),3.33-3.26(m,2H),3.03(s,3H),1.99-1.93(m,2H),1.85-1.77(m,2H),1.45(s,9H).

[0533]

[0534] tert-Butyl 4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl)oxy)piperidine-1-carboxylate (Intermediate AD): To a flask was added tert-butyl 4-((methylsulfonyl)oxy)piperidine-1-carboxylate (1.5 g), 6-hydroxy-4-oxachroman (565 mg), tetrabutylammonium chloride (207 mg), and potassium carbonate (1.6 g). After addition of water (17.5 mL) and DMF (1 mL), the reaction was heated to 100 °C for 4 hours. The reaction was allowed to cool to room temperature and extracted with DCM (2×). The combined extracts were washed with water and 2N NaOH, then dried (Na2SO4), filtered, and concentrated in vacuo. Purification by flash chromatography on silica gel (20%-70% EtOAc / hexanes) gave the title compound (472 mg): 1 H-NMR (400MHz, CDCl3) δ6.76(d,J=8.7Hz,1H),6.46(d,J=2.8Hz,1H),6.42(dd,J=8.7,2.8Hz,1H),4.35-4.28(m,1H),4.25-4.18( m,4H),3.69(ddd,J=12.9,7.3,3.5Hz,2H),3.29(ddd,J=12.5,7.9,3.8Hz,2H),1.90-1.85(m,2H),1.74-1.66(m,2H),1.46(s,9H). ES-MS[MC(CH3)3+H] + :280.4.

[0535] 4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl)oxy)piperidine (Intermediate AE): tert-Butyl 4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl)oxy)piperidine-1-carboxylate (315 mg) was dissolved in 4M HCl solution in 1,4-dioxane (14 mL). After 5 hours at room temperature, the reaction was concentrated in vacuo. The crude material was dissolved in MeOH and purified by HF SCX column and NH3 solution (2N in MeOH) to give the title compound (68 mg): 1 H NMR(400MHz,DMSO-d6)δ6.77(d,J=8.8Hz,1H),6.44-6.39(m,2H),4.48-4.46(m,1H),4.24-4.19(m,4H) ,3.31(ddd,J=12.8,12.8,3.7Hz,2H),3.16(ddd,J=8.2,8.2,3.9Hz,2H),2.20-2.05(m,4H); ES-MS[M+1] + :236.2.

[0536]

[0537] 2,2,3,3-Tetradeutero-1,4-benzodioxin-6-carboxaldehyde (Intermediate AG): In a 500mL round-bottom flask, 3,4-dihydroxybenzaldehyde (21.3g), 1,2-dibromoethane-D4 (14.1mL) and potassium carbonate (65.0g) were combined in acetone (515mL). The reaction was heated to reflux for 18h. The reaction was diluted with EtOAc, filtered through diatomaceous earth, and the filtrate was concentrated in vacuo. The crude sample was purified by flash chromatography on silica gel (0%-40% EtOAc / hexanes) to give the title compound (15.4g). 1 H NMR (400MHz, CDCl3) δ9.82 (s, 1H), 7.41-7.38 (m, 2H), 6.97 (d, J = 8.8Hz, 1H). ES-MS[M+1] + :169.

[0538]

[0539] 2,2,3,3-Tetradeutero-1,4-benzodioxin-6-ol (Intermediate AH): Step 1. To a solution of 2,2,3,3-tetradeutero-1,4-benzodioxin-6-carbaldehyde (15.4 g) in DCE (250 mL) was added 3-chloroperoxybenzoic acid (47.6 g, <77% CAS #937-14-4; Sigma-Aldrich). The reaction mixture was heated to 50° C. for 18 h. The reaction was diluted with DCM and saturated NaHCO3 solution. The layers were separated. The aqueous layer was extracted with DCM (2×) and the combined organic layers were washed with brine, dried (MgSO4), filtered and concentrated to give 2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4carboxylate (15.1 g). ES-MS[M+1] + :185.

[0540] Step 2. 2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4 carboxylate (15.1 g) was dissolved in methanol (250 mL) and potassium carbonate (15.5 g) was added. After 3 h, the solvent was removed and water / DCM (1:1) was added. The aqueous layer was slowly acidified to pH <4 by dropwise addition of 6N HCl aqueous solution. The layers were separated, and the aqueous layer was extracted with DCM (2×) and the combined organic layers were washed with brine, dried (MgSO4), filtered and concentrated. The filtrate was purified by flash chromatography on silica gel (0%-40% EtOAc / hexane). The desired fractions were concentrated and co-evaporated (with toluene×2) to give the title compound (12.3 g).1 H NMR (400MHz, CDCl3) δ6.72 (d, J = 8.8 Hz, 1H), 6.39 (d, J = 2.9 Hz, 1H), 6.34-6.31 (dd, J = 8.7, 2.9 Hz, 1H), 4.64 (s, 1H). ES-MS[M+1] + :157.

[0541]

[0542] tert-Butyl 4-[(2,2,3,3-tetradeutero-1,4-benzodioxin-6-yl)oxy]piperidine-1-carboxylate (Intermediate AI): To a 1000 mL round bottom flask was added tert-butyl 4-methylsulfonyloxypiperidine-1-carboxylate (18 g), 2,2,3,3-tetradeutero-1,4-benzodioxin-6-ol (6.0 g), potassium carbonate (16.2 g) and tetrabutylammonium chloride (2.1 g) in water (360 mL) and DMF (18 mL). The reaction was heated to reflux. After 24 h, the reaction was cooled to room temperature, diluted with EtOAc and washed with water (3×), brine (2×). The organic layer was dried (MgSO4), filtered and concentrated in vacuo. The crude oil was purified by silica gel flash chromatography (0%-40% EtOAc / hexanes) to give the title compound (3.1 g). 1 H NMR(400MHz, CDCl3) δ6.76(d,J=8.7Hz,1H),6.46(d,J=2.8Hz,1H),6.44-6.41(dd,J=8.7,2.8Hz,1H),4.3 2-4.27(m,1H),3.72-3.66(m,2H),3.31-3.25(m,2H),1.90-1.85(m,2H),1.74-1.66(m,2H),1.46(s,9H). ES-MS[M+1] + :340.

[0543] 4-[(2,2,3,3-tetradeutero-1,4-benzodioxin-6-yl)oxy]piperidine as TFA-salt (Intermediate AJ): To a round bottom flask containing tert-butyl 4-[(2,2,3,3-tetradeutero-1,4-benzodioxin-6-yl)oxy]piperidine-1-carboxylate (760 mg) in DCM (6 mL) was added trifluoroacetic acid (2 mL). After 2 h at room temperature, the solvent was removed and the crude residue was dissolved in DCM and concentrated in vacuo (3×) to give the title compound (802 mg). 1H NMR (400MHz, CDCl3) δ6.78 (d, J=8.9Hz, 1H), 6.45 (d, J=2.8Hz, 1H), 6.43-6.40 (dd, J=8.8 ,2.9Hz,1H),4.51-4.50(m,1H),3.44-3.37(m,2H),3.28-3.25(m,2H),2.16-2.07(m,4H). ES-MS[M+1] + :240.

[0544]

[0545] tert-Butyl 2,2,6,6-tetradeutero-4-[(2,2,3,3-tetradeutero-1,4-benzodioxin-6-yl)oxy]piperidine-1-carboxylate (Intermediate AK): In a vial, tert-butyl 2,2,6,6-tetradeutero-4-hydroxy-piperidine-1-carboxylate (100 mg, 0.49 mmol) [Reference: J. of Labelled Compounds and Radiopharmaceuticals 2018, 61, 1036-1042], triphenylphosphine (180 mg, 0.68 mmol) and 2,2,3,3-tetradeutero-1,4-benzodioxin-6-ol (107 mg, 0.68 mmol) were combined in THF (2 mL). The container was degassed and diisopropyl azodicarboxylate (0.13 mL, 0.68 mmol) was subsequently added. The reaction was heated at 60 °C for 18 h. The reaction was dissolved in 3:1 CHCl3:IPA and concentrated with diatomaceous earth. The solid was dry loaded on silica gel for purification (0%-25% EtOAc / hexane). The desired fraction was concentrated to obtain the title compound (123 mg). 1 H NMR (400MHz, CDCl3) δ6.75(d,J=8.7Hz,1H),6.45(d,J=2.8Hz,1H),6.42(dd,J=8.8 ,2.9Hz,1H),4.31-4.26(m,1H),1.87-1.83(m,2H),1.71-1.66(m,2H),1.45(s,9H). ES-MS[M+1] + :344.

[0546]

[0547] 2,2,6,6-Tetradeutero-4-[(2,2,3,3-tetradeutero-1,4-benzodioxin-6-yl)oxy]piperidine (Intermediate AL): In a vial, tert-butyl 2,2,6,6-tetradeutero-4-[(2,2,3,3-tetradeutero-1,4-benzodioxin-6-yl)oxy]piperidine-1-carboxylate (123 mg, 0.36 mmol) and trifluoroacetic acid (0.5 mL) were combined in dichloromethane (2 mL). The reaction was stirred for 1 h and concentrated. The oil was purified by SCX column (5G), washed with MeOH and eluted with 7N NH3 / MeOH solution. The solvent was removed to give the title compound (55 mg). 1 H NMR (400MHz, CDCl3) δ6.75(d,J=8.8Hz,1H),6.46(d,J=2.8Hz,1H),6.43(dd,J=8.8,2.8Hz,1H),4.24- 4.18(m,1H),1.99(d,J=3.8Hz,1H),1.96(d,J=3.7Hz,1H),1.63(d,J=8.4Hz,1H)1.60(d,J=8.6Hz,1H). ES-MS[M+1] + :244.

[0548]

[0549] 4-(2-Hydroxypropoxy)-3-iodo-benzoic acid methyl ester (Intermediate AM): To a 0°C solution of 1-bromo-2-propanol (1.04 mL) in DMF (22 mL) was added potassium carbonate (2.69 mL) and potassium iodide (0.59 g). After 15 min, 4-hydroxy-3-iodobenzoic acid methyl ester (2.44 g) was added and the reaction was heated to reflux. After 12 h, the reaction was cooled to room temperature and diluted with EtOAc and water. The layers were separated and the organic layer was washed with water (3×), brine (2×), dried (MgSO4) and concentrated to give the title compound (2.5 g). ES-MS [M+1] + :337.

[0550] 3-Methyl-2,3-dihydro-1,4-benzodioxin-6-carboxylic acid methyl ester (Intermediate AN): 4-(2-hydroxypropoxy)-3-iodo-benzoic acid methyl ester (2.5 g), cesium carbonate (4.88 g), palladium (II) acetate (0.14 g) and 2-di-tert-butylphosphino-2',4',6'-triisopropylbiphenyl (0.47 g) were combined in a 250 mL round bottom flask. The flask was evacuated and purged with anhydrous nitrogen (3×) followed by the addition of degassed toluene (18 mL). The reaction was sealed and heated to 110° C. After 5 h, the reaction was cooled to room temperature and the toluene was concentrated in vacuo. The crude oil was diluted with EtOAc and water. The layers were separated and the organic layer was washed with water (3×), brine (2×), dried over MgSO4 and concentrated. The crude oil was purified by silica gel flash chromatography (0%-20% EtOAc / hexanes) to give the title compound (0.34 g). 1 HNMR (400MHz, CDCl3) δ7.56-7.53(m,2H),6.92-6.86(m,1H),4.36-4.22(m,2H)3.89-3.82(m,1H)3.86(s,3H),1.37(d,J=6.5Hz,3H). ES-MS[M+1] + :209.

[0551]

[0552] (3-methyl-2,3-dihydro-1,4-benzodioxin-6-yl)methanol (intermediate AO): To a suspension of lithium aluminum hydride (1.01 mL, 1 M) in THF (1.2 mL) at 0°C was added 3-methyl-2,3-dihydro-1,4-benzodioxin-6-carboxylic acid methyl ester (200 mg) in THF (1.2 mL). The ice bath was removed, and after 1 h at room temperature, the reaction was cooled to 0°C and quenched with water (40 μL) and 10% aqueous NaOH (0.37 mL). After 10 min at 0°C, the suspension was filtered and the filtrate was concentrated in vacuo. The crude residue was purified by flash chromatography on silica gel (0%-50% EtOAc / hexanes), and the desired fractions were concentrated to give the title compound 148 mg. ES-MS [M+1] + :182.

[0553] 3-Methyl-2,3-dihydro-1,4-benzodioxin-6-carbaldehyde (Intermediate AP): To a vial containing (3-methyl-2,3-dihydro-1,4-benzodioxin-6-yl)methanol (148 mg) in DCM (2.7 mL) at 0°C was added Dess-Martin periodinane (418 mg). The ice bath was removed and after 18 h, the reaction was diluted with DCM and celite was added. The suspension was concentrated and then dry loaded for silica gel flash chromatography (0%-40% EtOAc / hexanes). The desired fractions were concentrated to give the title compound (129 mg). 1 H NMR (400MHz, CDCl3) δ9.82 (s, 1H), 7.41-7.38 (m, 2H), 6.99-6.95 (dd, J = 9.1, 5.0Hz, 1H), 4.38-4.15 (m, 2H) 3.92-3.82 (m, 1H) 1.37 (d, J = 6.4Hz, 3H). ES-MS[M+1] + :179.

[0554]

[0555] 3-Methyl-2,3-dihydro-1,4-benzodioxin-6-ol (Intermediate AQ): Step 1. To a solution of 3-methyl-2,3-dihydro-1,4-benzodioxin-6-carbaldehyde (129 mg) in DCE (1.4 mL) was added 3-chloroperoxybenzoic acid (187 mg, <77%, CAS #937-14-4; Sigma-Aldrich). The reaction was heated to 50 °C. After 3 h, the reaction was concentrated in vacuo, EtOAc was added to the residue, followed by saturated aqueous NaHCO3 (1.5 mL). The organic layer was separated, washed with brine, dried over MgSO4, filtered and concentrated in vacuo. The crude sample was purified by flash chromatography on silica gel (0%-30% EtOAc / hexanes) to give 3-methyl-2,3-dihydrobenzo[b][1,4]dioxin-6-ylcarboxylate (91 mg). ES-MS[M+1] + :195.

[0556] Step 2. 3-Methyl-2,3-dihydrobenzo[b][1,4]dioxin-6-ylcarboxylate (78 mg) was dissolved in methanol (1.4 mL) and potassium carbonate (121 mg) was added. After 45 min, the methanol was concentrated in vacuo. The residue was diluted with DCM and water. The layers were separated and the aqueous layer was extracted with DCM (2×) and the combined organic layers were washed with brine, dried over MgSO4, filtered and concentrated to give the title compound (71 mg). 1H NMR (400MHz, CDCl3) δ6.71 (d, J = 8.7Hz, 1H), 6.39-6.37 (m, 1H), 6.34-3.30 (m, 1H), 4.87 (s, 1H), 4.2 9-4.21(m,1H),4.15(dd,J=11.2,2.2Hz,1H),3.81-3.74(m,1H),1.33(d,J=6.5Hz,3H); ES-MS[M+1] + :167.

[0557] Analytical separation examples:

[0558] Chiral SFC separations were performed on a Thar (Waters) Investigator. Column: Chiral Technologies CHIRALPAK IE, 4.6x250 mm, 5 μm. Gradient conditions: 5% to 50% MeOH (MeOH modified with 0.1% DEA) in CO2 in 5 min, hold at 50% CO2 for 5 min. Flow rate: 3.5 mL / min. Column temperature: 40°C. System back pressure: 100 bar. Enantiomer N1:enantiomer N2 (1:1)

[0559] Preparative separation examples:

[0560] Chiral SFC separations were performed on a PIC Solution SFC-PICLab PREP 100. Column: Chiral Technologies CHIRALPAK IE, 20x250 mm, 5 μm. Conditions: 10% MeOH in CO2. Flow rate: 80 mL / min. Column temperature: 40°C. System back pressure: 100 bar.

[0561] Intermediate AS1 (first eluting peak):

[0562] Rt = 5.06 min (preparative method); ES-MS [M+Na] + =372.0; 100%ee.

[0563] Intermediate AS2 (second eluting peak):

[0564] Rt = 6.02 min (preparative method); ES-MS [M+Na] + =372.0; 97.3%ee.

[0565] tert-Butyl 4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl)oxy)-3-methylpiperidine-1-carboxylate (Intermediate AT): To a solution of 2,3-dihydrobenzo[b][1,4]dioxin-6-ol (0.2 g, 1.31 mmol) in THF (10 mL) was added tert-butyl 4-hydroxy-3-methylpiperidine-1-carboxylate (340 mg, 1.58 mmol) and PPh3 (641 mg, 2.45 mmol) at 20°C under N2 atmosphere with stirring. DIAD (399 mg, 1.97 mmol) was then added dropwise at 20°C. The mixture was stirred at 20°C for 16 hours. The reaction mixture was concentrated to give a residue. The residue was purified by silica gel flash chromatography (ISCO; 12 g SepaFlash silica flash column, 0%-10% ethyl acetate / petroleum ether gradient elution, 35 mL / min) to give the desired product (102 mg). 1H NMR (400 MHz, CDCl3) δ 6.77 (d, J = 8.8 Hz, 1H), 6.47 (d, J = 2.4 Hz, 1H), 6.44 (dd, J = 2.8 Hz, 8.8 Hz, 1H), 4.25-4.21 (m, 4H), 3.63-3.58 (m, 2H), 3.32-3.17 (m, 2H), 1.97-1.92 (m, 2H), 1.47 (s, 9H), 1.29-1.23 (m, 2H), 1.00 (d, J = 7.2 Hz, 3H).

[0566] 4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl)oxy)-3-methylpiperidine (Intermediate AT1): To a solution of tert-butyl 4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl)oxy)-3-methylpiperidine-1-carboxylate (102 mg, 0.29 mmol) in DCM (10 mL) was added TFA (3.08 g, 27.01 mmol). The mixture was stirred at 20 °C for 16 hours. The reaction mixture was concentrated to give 4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl)oxy)-3-methylpiperidine (98 mg) as 2,2,2-trifluoroacetate.

[0567]

[0568] 2,2,3,3-Tetradeutero-6-tetrahydropyran-2-yloxy-1,4-benzodioxin (Intermediate AU): To a solution of 2,2,3,3-tetradeutero-1,4-benzodioxin-6-ol (2.0 g) and pyridinium tosylate (48 mg) in DCM (32 mL) was added 3,4-dihydro-2H-pyran (1.8 mL). The reaction was stirred at room temperature for 3 h. The reaction was diluted with DCM and washed with 5% NaOH solution. The organic layer was dried (MgSO4), filtered and concentrated. The crude oil was purified by flash chromatography on silica gel (0%-30% EtOAc / hexanes) to give the title compound (2.61 g). 1 H NMR (400MHz, CDCl3) δ6.75(d,J=8.9Hz,1H),6.62(d,J=2.8Hz,1H),6.54(dd,J=8.8,2.8,1H),5.26(t,J=3.4,1 H),3.95-3.89(m,1H),3.60-3.55(m,1H),2.01-1.91(m,1H),1.84-1.80(m,2H),1.67-1.57(m,3H); ES-MS[M+1] + :241.

[0569]

[0570] 5-Bromo-2,2,3,3-tetradeutero-6-tetrahydropyran-2-yloxy-1,4-benzodioxin (Intermediate AV): To a solution of 2,2,3,3-tetradeutero-6-tetrahydropyran-2-yloxy-1,4-benzodioxin (1500 mg) in THF (62 mL) at -50°C was added n-butyllithium (7.49 mL, 2.5 M in hexanes) dropwise, and the reaction was stirred at -50°C for 2 h, followed by the slow addition of 1,2-dibromotetrafluoroethane (3.72 mL). The mixture was stirred for 1 h and then the cooling bath was removed. At room temperature, the reaction was quenched with water and extracted with ethyl acetate (3×). The combined organic layers were dried (MgSO4), filtered and concentrated. The crude oil was purified by silica gel flash chromatography (0%-15% EtOAc / hexanes) to give the title compound (1.63 g); 1 H NMR (400MHz, CDCl3) δ6.77(d,J=9.1Hz,1H),6.69(d,J=9.1Hz,1H),5.37(t,J=2.9Hz,1H),3.98-3.92(m,1H), 3.62-3.57(m,1H),2.13-1.97(m,1H),1.95-1.86(m,1H),1.85-1.82(m,1H),1.71-1.59(m,3H); ES-MS[M+Na]+ :341.

[0571]

[0572] 5-Bromo-2,2,3,3-tetradeutero-1,4-benzodioxin-6-ol (Intermediate AW): In a vial, 5-bromo-2,2,3,3-tetradeutero-6-tetrahydropyran-2-yloxy-1,4-benzodioxin (1632 mg), THF (50 mL) and 1N hydrochloric acid (30 mL) were combined. The reaction was stirred at room temperature for 1 h. The reaction was diluted with EtOAc and the layers were separated. The aqueous layer was extracted with EtOAc (2×) and the combined organic layers were washed with brine, dried (MgSO4), filtered and concentrated. The crude oil was purified by flash chromatography on silica gel (0%-20% EtOAc / hexanes) to give the title compound (1.10 g). 1 H NMR (400MHz, CDCl3) δ6.77(d,J=8.9Hz,1H),6.55(d,J=8.9Hz,1H),5.19(s,1H); ES-MS[M+1] + :235.

[0573]

[0574] tert-Butyl 4-[(5-bromo-2,2,3,3-tetradeutero-1,4-benzodioxin-6-yl)oxy]piperidine-1-carboxylate (Intermediate AX): In a round bottom flask, tert-butyl 4-methylsulfonyloxypiperidine-1-carboxylate (2.6 g), 5-bromo-2,2,3,3-tetradeutero-1,4-benzodioxin-6-ol (1.1 g), potassium carbonate (1.97 g) and tetrabutylammonium chloride (0.26 g) were combined in water (17 mL) and DMF (0.9 mL). The reaction was heated to 100 °C. After 18 h, the reaction was cooled, diluted with EtOAc and washed with water (3×), brine (2×), and the organic layer was dried (MgSO4), filtered and concentrated. The crude oil was purified by silica gel flash chromatography (0%-40% EtOAc / hexanes) to give the title compound (1.18 g). 1 H NMR(400MHz, CDCl3) δ6.76(d,J=9.1Hz,1H),6.47(d,J=8.9Hz,1H),4.40-4.39 (m,1H),3.68-3.62(m,2H),3.41-3.38(m,2H),1.82-1.81(m,4H),1.45(s,9H).

[0575]

[0576] tert-Butyl 4-[(2,2,3,3-tetradeutero-5-methyl-1,4-benzodioxin-6-yl)oxy]piperidine-1-carboxylate (Intermediate AY): In a vial, tert-butyl 4-[(5-bromo-2,2,3,3-tetradeutero-1,4-benzodioxin-6-yl)oxy]piperidine-1-carboxylate (100 mg), cesium carbonate (233 mg), [1,1'-bis(diphenyl)phosphino)ferrocene]dichloropalladium(II) (26 mg) and 2,4,6-trimethylboroxane (0.2 mL; 50% w / w solution in THF) were combined and the solid was degassed (3x) before adding 1,4-dioxane (2.3 mL). The reaction was heated to 80 °C. After 2 h, the reaction was filtered through a pad of celite and rinsed thoroughly with EtOAc / DCM. The filtrate was concentrated and purified by silica gel flash chromatography (0%-40% EtOAc / hexanes) to give the title compound (46 mg). ES-MS [M+1] + :354.

[0577]

[0578] 4-[(2,2,3,3-tetradeutero-5-methyl-1,4-benzodioxin-6-yl)oxy]piperidine (Intermediate AZ): In a vial, tert-butyl 4-[(2,2,3,3-tetradeutero-5-methyl-1,4-benzodioxin-6-yl)oxy]piperidine-1-carboxylate (46 mg), trifluoroacetic acid (0.2 mL) and DCM (2 mL) were combined. The reaction was stirred at room temperature for 1 h and concentrated. The crude residue was loaded onto an SCX column (strong cation exchanger; Agilent part number 14256027) in MeOH and washed with MeOH, then eluted with 7N NH3 / MeOH solution. The solvent was removed in vacuo to give the title compound (28 mg). 1 H NMR (400MHz, CDCl3) δ6.62(d,J=8.9Hz,1H),6.41(d,J=8.9Hz,1H),4.21-4.15(m,1H),3.15-3 .09(m,2H),2.73-2.66(m,2H),2.09(s,3H),1.99-1.94(m,2H),1.71-1.62(m,2H); ES-MS[M+1] + :254.

[0579]

[0580] 6-Bromo-5-fluoro-2,3-dihydro-1,4-benzodioxin (Intermediate BA): To a 0°C solution of 5-fluoro-2,3-dihydro-1,4-benzodioxin (900 mg) and sodium acetate (727 mg) in chloroform (9.7 mL) was added bromine (0.3 mL). After removing the ice bath and 2 h at room temperature, the reaction was treated with saturated aqueous NaS2O5, extracted with DCM (3×), passed through a phase separator and concentrated. The crude oil was purified by silica gel flash chromatography (0%-35% EtOAc / hexanes) to give the title compound as a 3:1 mixture of regioisomers (1.16 g). Major regioisomer (Intermediate BA): 1 H NMR (400MHz, CDCl3) δ, 6.95 (dd, J = 8.9, 6.9 Hz, 1H), 6.58 (dd, J = 8.9, 2.0 Hz, 1H), 4.32-4.30 (m, 2H), 4.28-4.26 (m, 2H).

[0581]

[0582] 2-(5-Fluoro-2,3-dihydro-1,4-benzodioxin-6-yl)-4,4,5,5-tetramethyl-1,3,2-dioxaborolane (Intermediate BB): To a vial was added 6-bromo-5-fluoro-2,3-dihydro-1,4-benzodioxin (715 mg), dichloro[1,1'-bis(diphenylphosphino)ferrocene]palladium(II) dichloromethane adduct (126 mg), potassium acetate (903 mg), and bis(pinacol)diboron (935 mg) in DMF (21 mL). After degassing the reaction (3x), the reaction was heated to 90 °C for 18 h. The reaction was cooled, diluted with EtOAc, filtered through celite and the filtrate was washed with water (3x), brine (2x), dried (MgSO4), filtered, and concentrated. The crude oil was purified by silica gel flash chromatography (0%-40% EtOAc / hexanes) to give the title compound (575 mg); ES-MS [M+1] + :281.

[0583]

[0584] 5-Fluoro-2,3-dihydro-1,4-benzodioxin-6-ol (Intermediate BC): To a solution of 2-(5-fluoro-2,3-dihydro-1,4-benzodioxin-6-yl)-4,4,5,5-tetramethyl-1,3,2-dioxaborolane (575 mg) in a mixture of 1N sodium hydroxide solution (6.1 mL) and THF (7.9 mL) at 0°C was added hydrogen peroxide (0.19 mL, 30% w / w). The reaction was stirred at room temperature for 1 h. The pH was adjusted to 3 with 1M HCl solution. The aqueous layer was extracted with EtOAc (3×) and the organic layer was washed with water, brine, dried (MgSO4), filtered and concentrated. The crude residue was purified by flash chromatography on silica gel (0%-40% EtOAc / hexanes) to give the title compound (124 mg); ES-MS [M+1] + :171.

[0585]

[0586] tert-Butyl 4-[(5-fluoro-2,3-dihydro-1,4-benzodioxin-6-yl)oxy]piperidine-1-carboxylate (Intermediate BD): To a vial was added tert-butyl 4-methylsulfonyloxy-piperidine-1-carboxylate (407 mg), 5-fluoro-2,3-dihydro-1,4-benzodioxin-6-ol (124 mg), potassium carbonate (306 mg) and tetrabutylammonium chloride (41 mg) in water (2.3 mL) and DMF (0.1 mL). The vial was sealed and heated to 100 ° C for 18 h. The reaction was cooled, diluted with EtOAc and washed with water, saturated NaHCO3 (2×), brine (2×), and the organic layer was dried (MgSO4), filtered and concentrated. The crude oil was purified by silica gel flash chromatography (0%-40% EtOAc / hexanes) to give the title compound (176 mg). 1 H NMR (400MHz, CDCl3) δ6.56 (dd, J=9.1, 1.9Hz, 1H), 6.49 (dd, J=9.1, 7.8Hz, 1H), 4.31-4.29 (m, 2H), 4.26-4.23 (m,2H),3.77-3.71(m,2H),3.29-3.23(m,2H),1.91-1.85(m,2H),1.77-1.69(m,2H),1.46(s,9H); ES-MS[M+1] + :354.

[0587]

[0588] 4-((5-Fluoro-2,3-dihydrobenzo[b][1,4]dioxin-6-yl)oxy)piperidin-1-ium 2,2,2-trifluoroacetic acid (Intermediate BE): To a vial was added tert-butyl 4-[(5-fluoro-2,3-dihydro-1,4-benzodioxin-6-yl)oxy]piperidine-1-carboxylate (176 mg), trifluoroacetic acid (0.5 mL) and DCM (2.5 mL). After 1 h at room temperature, the reaction was concentrated in vacuo and co-evaporated with DCM (2x) to give the title compound (202 mg). 1 H NMR(400MHz,MeOD)δ6.63(dd,J=9.1,7.8Hz,1H),6.58(dd,J=9.2,1.6Hz,1H),4.48-4.44(m,1H),4.28-4.26(m, 2H),4.23-4.21(m,2H),3.44-3.37(m,2H),3.22-3.17(m,2H),2.14-2.05(m,2H),2.04-1.97(m,2H); ES-MS[M+1] + :254.

[0589]

[0590] 2,2,3,3-Tetradeutero-5-fluoro-1,4-benzodioxin (Intermediate BF): To a solution of 3-fluorocatechol (2.0 g) in acetone (52 mL) was added potassium carbonate (6.6 g) followed by 1,2-dibromoethane-D4 (1.43 mL). The reaction was heated at 60 ° C for 18 h. The reaction was filtered through celite and the filtrate was concentrated. The crude residue was purified by flash chromatography on silica gel (0%-40% EtOAc / hexanes) to give the title compound (1.54 g). 1 H NMR (400MHz, CDCl3) δ6.76-6.70 (m, 1H), 6.69-6.64 (m, 2H).

[0591]

[0592] 6-Bromo-2,2,3,3-tetradeutero-5-fluoro-1,4-benzodioxin (Intermediate BG): To a solution of 2,2,3,3-tetradeutero-5-fluoro-1,4-benzodioxin (500 mg) and sodium acetate (394 mg) in chloroform (5.3 mL) at 0°C was added bromine (162 μL) dropwise. The ice bath was removed and the reaction was stirred at room temperature for 2 h. The reaction was treated with saturated NaS2O5, extracted with DCM (3×), passed through a phase separator and concentrated. The crude residue was purified by flash chromatography on silica gel (0%-20% EtOAc / hexanes) to give the title compound (660 mg, 3:1 mixture of regioisomers). Major regioisomer (Intermediate BG): 1 H NMR (400MHz, CDCl3) δ6.96 (dd, J=9.0, 7.0Hz, 1H), 6.59 (dd, J=9.0, 2.0, 1H).

[0593]

[0594] 4,4,5,5-Tetramethyl-2-(2,2,3,3-tetradeutero-5-fluoro-1,4-benzodioxin-6-yl)-1,3,2-dioxaborolane (Intermediate BH): To a vial was added bis(pinacol)diboron (848 mg), 6-bromo-2,2,3,3-tetradeutero-5-fluoro-1,4-benzodioxin (660 mg), dichloro[1,1'-bis(diphenylphosphino)-ferrocene]palladium(II) dichloromethane adduct (114 mg) and potassium acetate (820 mg) in DMF (9.3 mL). After the reaction was degassed, the reaction was heated to 90 °C for 18 h. The reaction was allowed to cool to room temperature and diluted with EtOAc, filtered through celite and the filtrate washed with water (3x), brine (2x), dried (MgSO4), filtered and concentrated. The crude residue was purified by silica gel flash chromatography (0%-40% EtOAc / hexanes) to give the title compound (494 mg). 1 H NMR (400MHz, CDCl3) δ7.16 (dd, J=8.4, 6.1Hz, 1H), 6.64 (dd, J=8.3, 1.4Hz, 1H), 1.33 (s, 12H); ES-MS [M+1] + :285.

[0595]

[0596] 2,2,3,3-Tetradeutero-5-fluoro-1,4-benzodioxin-6-ol (Intermediate BI): To a solution of 4,4,5,5-tetramethyl-2-(2,2,3,3-tetradeutero-5-fluoro-1,4-benzodioxin-6-yl)-1,3,2-dioxaborolane (494 mg) in a mixture of sodium hydroxide solution (5.2 mL, 1 M) and THF (6.7 mL) at 0°C was added hydrogen peroxide (160 μL, 30% w / w). After the reaction was stirred at room temperature for 1 h, the pH was adjusted to 3 with 1 M aqueous HCl. The aqueous layer was extracted with EtOAc (3×) and the combined organic layers were washed with water, brine, dried (MgSO4), filtered and concentrated. The crude residue was purified by flash chromatography on silica gel (0%-40% EtOAc / hexanes) to give the title compound (116 mg). 1 H NMR (400MHz, CDCl3) δ6.55 (dd, J=9.1, 2.1Hz, 1H), 6.47 (t, J=8.8Hz, 1H), 4.27 (s, 1H); ES-MS [M+1] + :175.

[0597]

[0598] tert-Butyl 4-[(2,2,3,3-tetradeutero-5-fluoro-1,4-benzodioxin-6-yl)oxy]piperidine-1-carboxylate (Intermediate BJ): tert-Butyl 4-methylsulfonyloxypiperidine-1-carboxylate (369 mg), 2,2,3,3-tetradeutero-5-fluoro-1,4-benzodioxin-6-ol (115 mg), potassium carbonate (278 mg) and tetrabutylammonium chloride (37 mg) in water (2.0 mL) and DMF (0.1 mL) were added to a vial. The vial was sealed and heated to 100 °C for 18 h. The reaction was cooled, diluted with EtOAc and washed with water, saturated NaHCO3 solution, brine (2×), and the organic layer was dried (MgSO4), filtered and concentrated. The crude residue was purified by silica gel flash chromatography (0%-40% EtOAc / hexanes) to give the title compound (124 mg). 1 H NMR (400MHz, CDCl3) δ6.56 (dd, J=9.1, 1.9Hz, 1H), 6.50 (dd, J=9.1, 7.9Hz, 1H), 4.26 (m, 1H), 3.77 -3.71(m,2H),3.29-3.23(m,2H),1.91-1.85(m,2H),1.78-1.70(m,2H),1.47(s,9H); ES-MS[M+1] + :358.

[0599]

[0600] 4-[(2,2,3,3-Tetradeutero-5-fluoro-1,4-benzodioxin-6-yl)oxy]piperidine; 2,2,2-trifluoroacetic acid (Intermediate BK): To a vial was added tert-butyl 4-[(5-fluoro-2,3-dihydro-1,4-benzodioxin-6-yl)oxy]piperidine-1-carboxylate (124 mg), trifluoroacetic acid (0.3 mL) and DCM (1.7 mL). After 1 h at room temperature, the reaction was concentrated in vacuo and co-evaporated with DCM (2×) to give the title compound (158 mg). 1 H NMR (400MHz, CD3OD) δ6.63 (dd, J=9.1, 7.8Hz, 1H), 6.58 (dd, J=9.2, 1.6Hz, 1H), 4.49 -4.44(m,1H),3.43-3.37(m,2H),3.22-3.17(m,2H),2.13-1.98(m,4H); ES-MS[M+1] + :258.

[0601]

[0602] 4-deuterated-4-hydroxy-piperidine-1-carboxylic acid tert-butyl ester (Intermediate BL): Sodium borodeuteride (3.2 mL, 100 mmol) was added to a 0°C solution of 1-tert-butyl-4-piperidone (10 g, 50 mmol) in methanol (250 mL). The resulting mixture was stirred at room temperature for 4 h. The reaction was quenched with saturated NH4Cl (aq.) and extracted with EtOAc (3x). The combined organic layers were dried (MgSO4), filtered and concentrated to give the title compound 10 g (99%). 1 H NMR (400MHz, CDCl3) δ3.85 (d, J = 12.4Hz, 2H), 3.06-3.00 (m, 2H), 1.87-1.82 (m, 2H), 1.48-1.42 (m, 2H), 1.46 (s, 9H).

[0603]

[0604] 4-deuterated-4-(p-tolylsulfonyloxy)piperidine-1-carboxylic acid tert-butyl ester (Intermediate BM): To a suspension of 4-deuterated-4-hydroxy-piperidine-1-carboxylic acid tert-butyl ester (10 g) and 4-dimethyl-aminopyridine (0.6 g) in pyridine (45 mL) was added p-toluenesulfonyl chloride (11.8 g). The mixture was stirred at room temperature for 18 h. The reaction was quenched with saturated NaHCO3 solution and extracted with EtOAc (2x). The combined organic layers were washed with water (2x), brine (2x), dried (MgSO4), filtered and concentrated. The crude oil was purified by normal phase column chromatography (0%-20% EtOAc / hexane). After the solvent was removed, the desired compound 14.3 g (81%) was obtained. 1 H NMR(400MHz, CDCl3) δ7.79(d,J=8.4Hz,2H),7.34(d,J=8.0Hz,2H),3.61-3.55(m,2H) ,3.28-3.22(m,2H),2.45(s,3H),1.79-1.73(m,2H),1.70-1.64(m,2H),1.43(s,9H). ES-MS[M+Na] + :379.

[0605]

[0606] 4-Deutero-4-[(2,2,3,3-tetradeutero-1,4-benzodioxin-6-yl)oxy]piperidine (Intermediate BN): To a round bottom flask was added 2,2,3,3-tetradeutero-1,4-benzodioxin-6-ol (1.0 g, 6.7 mmol), tert-butyl 4-deutero-4-(p-toluenesulfonyloxy)piperidine-1-carboxylate (2.0 g, 5.6 mmol), potassium carbonate (2.4 g, 16.8 mmol), and tetrabutylammonium chloride (0.31 g, 1.1 mmol) in water (25 mL) and DMF (1.3 mL). The reaction was heated at reflux for 18 h. The reaction was diluted with 3:1 CHCl3 / IPA and the layers were separated. The aqueous layer is extracted with 3:1CHCl3 / IPA (2x) and the combined organic layer is washed with water, brine, then dried (MgSO4), filtered and concentrated. The crude oil is purified by normal phase column chromatography (0%-20% EtOAc / hexane). After solvent removal, oil is dissolved in DCM (9mL), trifluoroacetic acid (2.1mL, 28mmol) is subsequently added. After 1h, the solvent is removed in a vacuum. Residue is dissolved in MeOH and loaded on SCX posts. Post is rinsed with MeOH and 7N NH3 / MeOH solution. Solvent is removed to obtain title compound 725mg (53%). 1H NMR (400MHz, CDCl3) δ6.75(d,J=8.7,1H),6.45(d,J=2.8,1H),6.41(dd,J=8.8,2.9H z,1H),3.21-3.15(m,2H),2.87-2.81(m,2H),2.07-2.00(m,2H),1.78-1.72(m,2H). ES-MS[M+1] + :241.

[0607]

[0608] (2R,4S)-tert-Butyl 4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)-2-methylpiperidine-1-carboxylate (Intermediate BO): To a mixture of 2,3-dihydrobenzo[b][1,4]dioxin-2,2,3,3-d4-6-ol (5 g, 32.02 mmol), (2R,4R)-tert-butyl 4-hydroxy-2-methylpiperidine-1-carboxylate (6.89 g) and PPh3 (10.93 g) in THF (100 mL) was slowly added DIAD (8.1 mL) at 20°C and the reaction was stirred at 20°C for 16 hours. The reaction was concentrated and the residue was purified by Combi Flash (silica gel, from 0% to 30%, ethyl acetate in petroleum ether) to give tert-butyl (2R,4S)-4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)-2-methylpiperidine-1-carboxylate (3.7 g). 1 H NMR (400MHz, CDCl3) δ6.77(d,J=8.8Hz,1H),6.46(s,1H),6.42(dd,J=8.8Hz,1.6Hz,1H),4.61-4.46(m,1H),4.41-4.31(m,1H),4.13- 4.06(m,1H),2.96-2.89(m,1H),2.14-2.04(m,1H),2.01-1.90(m,1H),1.74-1.64(m,1H),1.52-1.42(s,10H),1.19(d,J=7.2Hz,3H).

[0609]

[0610] (2R,4S)-4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)-2-methylpiperidine (Intermediate BP): To a solution of tert-butyl (2R,4S)-4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)-2-methylpiperidine-1-carboxylate (3.7 g) in DCM (20 mL) was added TFA (4 mL) and the reaction was stirred at 20° C. for 3 hours. The reaction was quenched with saturated aqueous Na2CO3 solution to pH 8-9 at 0° C. and extracted with DCM (20 mL×2). The combined organic layers were washed with brine (20 mL), dried over Na2SO4 and concentrated to give (2R,4S)-4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)-2-methylpiperidine (2.6 g, crude product). ES-MS [M+1] + :254.2.

[0611]

[0612] 9-Bromo-7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl)oxy)piperidin-1-yl)-8-methyl-4H-pyrimido[1,2-b]pyridazin-4-one (Intermediate BQ): To a solution of 9-bromo-7-chloro-8-methyl-4H-pyrimido[1,2-b]pyridazin-4-one (2.27 g) in DMF (30 mL) was added 4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl)oxy)piperidine (1.98 g) and K2CO3 (1.14 g). The mixture was stirred at 25°C for 16 hours. The reaction mixture was concentrated and the residue was purified by silica gel flash chromatography ( ; 220g The mixture was purified by silica flash column, 0%-100% ethyl acetate / petroleum ether gradient elution, 100 mL / min) to give 9-bromo-7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl)oxy)piperidin-1-yl)-8-methyl-4H-pyrimido[1,2-b]pyridazin-4-one (750 mg). 1HNMR (400MHz, CDCl3) δ8.22(d,J=6.4Hz,1H),6.79(d,J=8.8Hz,1H),6.60(d,J=6.0Hz,1H),6.51-6.44(m,2H),4.43-4. 39(m,1H),4.28-4.20(m,4H),3.64-3.54(m,2H),3.30-3.20(m,2H),2.56(s,3H),2.20-2.09(m,2H),2.04-1.93(m,2H).

[0613] b. Exemplary compounds of the present invention

[0614] Example 1. 7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)piperidin-1-yl)-8-methyl-4H-pyrimido[1,2-b]pyridazin-4-one (Compound 1)

[0615]

[0616] To a solution of 7-chloro-8-methyl-pyrimido[1,2-b]pyridazine-4-one (15 mg) in tert-butanol (1 mL) was added N,N-diisopropylethylamine (67 μL) and 4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)piperidine (28 mg). The reaction mixture was heated to 120 ° C for 18 hours. The crude product was dissolved in DMSO (1 mL) and purified by reverse phase chromatography using Gilson HPLC (30×100 mm column, 10%-55% ACN / 0.05% NH4OH aqueous solution, running 8 min). The fractions containing the product were concentrated to give the title compound 15 mg as a solid.

[0617] 1 H NMR (400MHz, DMSO) δ8.11(d,J=6.3Hz,1H),7.74(q,J=1.2Hz,1H),6.76(d,J=8.8Hz,1H),6.54(d,J=2.8Hz,1H),6.49(dd,J=8.8,2.9Hz,1H),6.4 0(d,J=6.3Hz,1H),4.50(hept,J=3.8Hz,1H),3.64-3.46(m,2H),3.21-3 .10(m,2H),2.42(d,J=1.2Hz,3H),2.10-2.01(m,2H),1.84-1.71(m,2H). ES-MS[M+1] + :399.

[0618] Example 2. 7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)piperidin-1-yl)-8,9-dimethyl-4H-pyrimido[1,2-b]pyridazin-4-one (Compound 2)

[0619]

[0620] To a solution of 7-chloro-8,9-dimethyl-pyrimido[1,2-b]pyridazine-4-one (10 mg) and 4-[(2,2,3,3-tetradeuterated-1,4-benzodioxin-6-yl)oxy]piperidine (17 mg) in tert-butanol (1 mL) was added N,N-diisopropylethylamine (42 μL). The mixture was heated to 120 ° C for 18 hours. The solvent was removed and the crude product was dissolved in DMSO (2 mL) and purified by reverse phase chromatography using Gilson HPLC (30×100 mm column, 25%-60% ACN / 0.05% NH4OH aqueous solution, running 8 min). The fractions containing the product were concentrated to give the title compound (11 mg; 53% yield) as a solid. 1 H NMR (400MHz, CDCl3) δ8.15(d,J=6.3Hz,1H),6.78(d,J=8.7Hz,1H),6.55(d,J=6.3Hz,1H),6.49(d,J=2.8Hz,1H),6.45(dd,J=8.7,2.9Hz,1H),4.3 8(hept,J=3.7Hz,1H),3.61-3.46(m,2H),3.24-3.14(m,2H),2.53(d,J= 0.5Hz, 3H), 2.37 (d, J = 0.5Hz, 3H), 2.19-2.09 (m, 2H), 2.02-1.90 (m, 2H). ES-MS[M+1] + :413.

[0621] Example 3. 7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)piperidin-1-yl)-2,8-dimethyl-4H-pyrimido[1,2-b]pyridazin-4-one (Compound 3)

[0622]

[0623] To a solution of 7-chloro-2,8-dimethyl-pyrimido[1,2-b]pyridazine-4-one (15 mg) and 4-[(2,2,3,3-tetradeuterated-1,4-benzodioxin-6-yl)oxy]piperidine (26 mg) in tert-butanol (1 mL) was added N,N-diisopropylethylamine (62 μL). The mixture was heated to 120 ° C for 18 hours, then cooled to room temperature and concentrated. The crude product was dissolved in DMSO (2 mL) and purified by reverse phase chromatography using Gilson HPLC (30×100 mm column, 15%-55% ACN / 0.05% NH4OH aqueous solution, running 8 min). The fractions containing the product were concentrated to give the title compound (14 mg; 47% yield) as a solid. 1 H NMR (400MHz, CDCl3) δ7.44(q,J=1.0Hz,1H),6.78(d,J=8.7Hz,1H),6.49(d,J=2.8Hz,1H),6.45(dd,J=8.7,2.9Hz,1H),6.42(s,1H),4.3 9(hept,J=3.5Hz,1H),3.66-3.55(m,2H),3.30-3.19(m,2H),2.43(d,J=1.2Hz,3H),2.39(s,3H),2.17-2.03(m,2H),2.03-1.89(m,2H). ES-MS[M+1] + :413.

[0624] Example 4. 7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)piperidin-1-yl)-2,3,8-trimethyl-4H-pyrimido[1,2-b]pyridazin-4-one (Compound 4)

[0625]

[0626] To a solution of 7-chloro-2,3,8-trimethyl-pyrimido[1,2-b]pyridazine-4-one (10 mg) and 4-[(2,2,3,3-tetradeuterated-1,4-benzodioxin-6-yl)oxy]piperidine (16 mg) in tert-butanol (1 mL) was added N,N-diisopropylethylamine (40 μL). The mixture was heated to 120 ° C for 18 hours, then cooled to room temperature and concentrated. The crude product was dissolved in DMSO (2 mL) and purified by reverse phase chromatography using Gilson HPLC (30×100 mm column, 20%-60% ACN / 0.05% NH4OH aqueous solution, running 8 min). The fractions containing the product were concentrated to give the title compound (11 mg; 56% yield) as a solid. 1H NMR (400MHz, CDCl3) δ7.40(q,J=1.2Hz,1H),6.78(d,J=8.7Hz,1H),6.49(d,J=2.8Hz,1H),6.45(dd,J=8.7,2.8Hz,1H),4.38(hept,J=3. 6Hz,1H),3.65-3.55(m,2H),3.29-3.18(m,2H),2.42(s,3H),2.40(d,J=1.2Hz,3H),2.23(s,3H),2.18-2.07(m,2H),2.01-1.88(m,2H). ES-MS[M+1] + :427.

[0627] Example 5. 7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)piperidin-1-yl)-2,8,9-trimethyl-4H-pyrimido[1,2-b]pyridazin-4-one (Compound 5)

[0628]

[0629] To a solution of 7-chloro-2,8,9-trimethyl-pyrimido[1,2-b]pyridazin-4-one (10 mg) and 4-[(2,2,3,3-tetradeutero-1,4-benzodioxin-6-yl)oxy]piperidine (16 mg) in tert-butanol (1 mL) was added N,N-diisopropylethylamine (40 μL). The mixture was heated to 120° C. for 18 hours. Additional N,N-diisopropylethylamine (40 μL) and 4-[(2,2,3,3-tetradeutero-1,4-benzodioxin-6-yl)oxy]piperidine (16 mg) were added and the reaction was stirred at 120° C. for another 18 hours. The solvent was removed and the crude product was dissolved in DMSO (2 mL) and purified by reverse phase chromatography using a Gilson HPLC (30×100 mm column, 30%-70% ACN / 0.05% NH 4 OH in water, 8 min run). The product-containing fractions were concentrated to give the title compound (12 mg; 61% yield) as a solid. 1H NMR (400MHz, CDCl3) δ6.78(d,J=8.7Hz,1H),6.49(d,J=2.8Hz,1H),6.45(dd,J=8.7,2.9Hz,1H),6.42(s,1H),4.37(hept,J=3. 7Hz,1H),3.59-3.47(m,2H),3.22-3.12(m,2H),2.53(s,3H),2.42(s,3H),2.35(s,3H),2.18-2.08(m,2H),2.01-1.88(m,2H). ES-MS[M+1] + :427.

[0630] Example 6. 7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)piperidin-1-yl)-8-methyl-2-(trifluoromethyl)-4H-pyrimido[1,2-b]pyridazin-4-one (Compound 6)

[0631]

[0632] To a solution of 7-chloro-8-methyl-2-(trifluoromethyl)pyrimido[1,2-b]pyridazine-4-one (13 mg) and 4-[(2,2,3,3-tetradeuterated-1,4-benzodioxin-6-yl)oxy]piperidine (18 mg) in tert-butanol (1 mL) was added N,N-diisopropylethylamine (43 μL). The mixture was heated to 120 ° C for 18 hours and then concentrated. The crude product was dissolved in DMSO (2 mL) and purified by reverse phase chromatography using Gilson HPLC (30×100 mm column, 25%-65% ACN / 0.05% NH4OH aqueous solution, running 8 min). The fraction containing the product was concentrated to give the title compound (12 mg; 54% yield) as a solid. 1 HNMR (400MHz, CDCl3) δ7.62(q,J=1.1Hz,1H),6.88(s,1H),6.78(d,J=8.7Hz,1H),6.49(d,J=2.8Hz,1H),6.45(dd,J=8.7,2.9Hz,1 H), 4.42 (hept, J = 3.4Hz, 1H), 3.70-3.59 (m, 2H), 3.37-3.26 (m, 2H), 2.48 (d, J = 1.2Hz, 3H), 2.17-2.04 (m, 2H), 2.04-1.94 (m, 2H). ES-MS[M+1] + :467.

[0633] Example 7. 7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)piperidin-1-yl)-8,9-dimethyl-2-(trifluoromethyl)-4H-pyrimido[1,2-b]pyridazin-4-one (Compound 7)

[0634]

[0635] To a solution of 7-chloro-8,9-dimethyl-2-(trifluoromethyl)pyrimido[1,2-b]pyridazine-4-one (10 mg) and 4-[(2,2,3,3-tetradeuterated-1,4-benzodioxin-6-yl)oxy]piperidine (13 mg) in tert-butanol (1 mL) was added N,N-diisopropylethylamine (32 μL). The mixture was heated to 120 ° C for 18 hours. The solvent was removed and the crude product was dissolved in DMSO (2 mL) and purified by reverse phase chromatography using Gilson HPLC (30×100 mm column, 25%-65% ACN / 0.05% NH4OH aqueous solution, running 8 min). The fractions containing the product were concentrated to give the title compound (15.5 mg; 90% yield) as a solid. 1 H NMR (400MHz, CDCl3) δ6.92(s,1H),6.79(d,J=8.7Hz,1H),6.50(d,J=2.8Hz,1H),6.46(dd,J=8.8,2.9Hz,1H),4.39(hept,J=3.6H z,1H),3.51-3.37(m,2H),3.14-3.03(m,2H),2.48(d,J=0.6Hz,3H),2.34(d,J=0.6Hz,3H),2.15-2.03(m,2H),2.01-1.88(m,2H). ES-MS[M+1] + :481.

[0636] Example 8. 2-(Difluoromethyl)-7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)piperidin-1-yl)-8-methyl-4H-pyrimido[1,2-b]pyridazin-4-one (Compound 8)

[0637]

[0638] To a solution of 7-chloro-2-(difluoromethyl)-8-methyl-pyrimido[1,2-b]pyridazine-4-one (10 mg) and 4-[(2,2,3,3-tetradeuterated-1,4-benzodioxin-6-yl)oxy]piperidine (15 mg) in tert-butanol (1 mL) was added N,N-diisopropylethylamine (36 μL). The mixture was heated to 120 ° C for 18 hours. The solvent was removed and the crude product was dissolved in DMSO (2 mL) and purified by reverse phase chromatography using Gilson HPLC (30×100 mm column, 20%-60% ACN / 0.05% NH4OH aqueous solution, running 8 min). The fractions containing the product were concentrated to give the title compound (13 mg; 70% yield) as a solid. 1 H NMR (400MHz, CDCl3) δ7.56(q,J=1.2Hz,1H),6.80(d,J=8.8Hz,1H),6.78(d,J=8.7Hz,1H),6.49(d,J=2.8Hz,1H),6.45(dd,J=8.8,2.8Hz,1H),6.4 4(t,J=55.0Hz,1H),4.41(hept,J=3.5Hz,1H),3.69-3.58(m,2H),3.35-3 .24(m,2H),2.47(d,J=1.2Hz,3H),2.18-2.07(m,2H),2.04-1.91(m,2H). ES-MS[M+1] + :449.

[0639] Example 9. 2-(Difluoromethyl)-7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)piperidin-1-yl)-8,9-dimethyl-4H-pyrimido[1,2-b]pyridazin-4-one (Compound 9)

[0640]

[0641] To a solution of 7-chloro-2-(difluoromethyl)-8,9-dimethyl-pyrimido[1,2-b]pyridazine-4-one (10 mg) and 4-[(2,2,3,3-tetradeuterated-1,4-benzodioxin-6-yl)oxy]piperidine (14 mg) in tert-butanol (1 mL) was added N,N-diisopropylethylamine (34 μL). The mixture was heated to 120 ° C for 18 hours. The solvent was removed and the crude product was dissolved in DMSO (2 mL) and purified by reverse phase chromatography using Gilson HPLC (30×100 mm column, 30%-70% ACN / 0.05% NH4OH aqueous solution, running 8 min). The fractions containing the product were concentrated to give the title compound (14 mg; 76% yield) as a solid.1 H NMR (400MHz, CDCl3) δ6.82(s,1H),6.78(d,J=8.7Hz,1H),6.53(t,J=55.0Hz,1H),6.49(d,J=2.8Hz,1H),6.45(dd,J=8.7,2.9Hz,1H ), 4.39(hept,J=3.5Hz,1H),3.62-3.52(m,2H),3.32-3.13(m,2H),2.54(s,3H),2.38(s,3H),2.21-2.08(m,2H),2.05-1.90(m,2H). ES-MS[M+1] + :463.

[0642] Example 10. 2-Cyclopropyl-7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)piperidin-1-yl)-8-methyl-4H-pyrimido[1,2-b]pyridazin-4-one (Compound 10)

[0643]

[0644] To a solution of 7-chloro-2-cyclopropyl-8-methyl-pyrimido[1,2-b]pyridazine-4-one (10 mg) and 4-[(2,2,3,3-tetradeuterated-1,4-benzodioxin-6-yl)oxy]piperidine (15 mg) in tert-butanol (1 mL) was added N,N-diisopropylethylamine (37 μL). The mixture was heated to 120 ° C for 18 hours. The solvent was removed and the crude product was dissolved in DMSO (2 mL) and purified by reverse phase chromatography using Gilson HPLC (30×100 mm column, 25%-65% ACN / 0.05% NH4OH aqueous solution, running 8 min). The fractions containing the product were concentrated to give the title compound (8.4 mg; 45% yield) as a solid. 1 H NMR (400MHz, CDCl3) δ7.39 (q, J=1.2Hz, 1H), 6.78 (d, J=8.7Hz, 1H), 6.49 (d, J= 2.8Hz,1H),6.45(dd,J=8.7,2.9Hz,1H),6.41(s,1H),4.37(hept,J=3.7Hz,1H) ,3.64-3.53(m,2H),3.27-3.17(m,2H),2.40(d,J=1.2Hz,3H),2.17-2.05(m,2H ),2.03-1.91(m,2H),1.90-1.82(m,1H),1.13-1.04(m,2H),1.03-0.95(m,2H). ES-MS[M+1] +:439.

[0645] Example 11. 7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)piperidin-1-yl)-2-ethyl-8,9-dimethyl-4H-pyrimido[1,2-b]pyridazin-4-one (Compound 11)

[0646]

[0647] N, N-diisopropylethylamine (55 μL) was added to a solution of 7-chloro-2-ethyl-8,9-dimethyl-pyrimido[1,2-b]pyridazine-4-one (15 mg) and 4-(2,3-dihydro-1,4-benzodioxin-6-yloxy)piperidine (28 mg) in tert-butanol (0.5 mL). The mixture was heated to 120 ° C for 18 hours. The solvent was removed and the crude product was dissolved in DMSO (1 mL) and purified by reverse phase chromatography using Gilson HPLC (30 × 100 mm column, 30%-75% ACN / 0.1% TFA aqueous solution, running 8 min). The fraction containing the product was alkalized with saturated NaHCO3 and extracted with 4:1 chloroform / IPA. The organic extract was passed through a phase separator and concentrated to give the title compound (11 mg, 41% yield) as a solid. 1 H NMR (400MHz, CDCl3) δ6.77(d,J=8.7Hz,1H),6.49(d,J=2.8Hz,1H),6.45(dd, 1H),6.44(s,1H),4.36(hept,J=3.7Hz,1H),4.27-4.23(m,2H),4.23-4.19(m ,2H),3.59-3.48(m,2H),3.22-3.11(m,2H),2.67(q,J=7.5Hz,2H),2.52(s,3 H), 2.35 (s, 3H), 2.21-2.08 (m, 2H), 2.05-1.87 (m, 2H), 1.29 (t, J = 7.6Hz, 3H). ES-MS[M+1] + :437.

[0648] Example 12. 7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl)oxy)piperidin-1-yl)-2-isopropyl-8,9-dimethyl-4H-pyrimido[1,2-b]pyridazin-4-one (Compound 12)

[0649]

[0650] N, N-diisopropylethylamine (52 μL) was added to a solution of 7-chloro-2-isopropyl-8,9-dimethyl-pyrimido[1,2-b]pyridazine-4-one (15 mg) and 4-(2,3-dihydro-1,4-benzodioxin-6-yloxy)piperidine (28 mg) in tert-butanol (0.5 mL). The mixture was heated to 120 ° C for 18 hours. The solvent was removed and the crude product was dissolved in DMSO (1 mL) and purified by reverse phase chromatography using Gilson HPLC (30 × 100 mm column, 20%-65% ACN / 0.1% TFA aqueous solution, running 8 min). The fraction containing the product was alkalized with saturated NaHCO3 and extracted with 4:1 chloroform / IPA. The organic extract was passed through a phase separator and concentrated to give the title compound (21 mg, 79% yield) as a solid. 1 H NMR(400MHz, CDCl3) δ6.79(d,J=8.7Hz,1H),6.50(d,J=2.8Hz,1H),6.46(dd,1H ),6.45(s,1H),4.39(tt,J=7.1,3.6Hz,1H),4.28-4.24(m,2H),4.23-4.20(m,2 H),3.66(hept,J=6.9Hz,1H),3.43-3.32(m,2H),3.10-2.97(m,2H),2.49(s,3H ),2.32(s,3H),2.24-2.06(m,2H),2.04-1.90(m,2H),1.31(s,3H),1.29(s,3H). ES-MS[M+1] + :451.

[0651] Example 13. 7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl)oxy)piperidin-1-yl)-2-(methoxymethyl)-8,9-dimethyl-4H-pyrimido[1,2-b]pyridazin-4-one (Compound 13)

[0652]

[0653] To a solution of 7-chloro-2-(methoxymethyl)-8,9-dimethyl-pyrimido[1,2-b]pyridazine-4-one (15 mg) and 4-(2,3-dihydro-1,4-benzodioxin-6-yloxy)piperidine (28 mg) in tert-butanol (0.5 mL) was added N,N-diisopropylethylamine (52 μL). The mixture was heated to 120 ° C for 18 hours. The solvent was removed and the crude product was dissolved in DMSO (1 mL) and purified by reverse phase chromatography using Gilson HPLC (30×100 mm column, 25%-65% ACN / 0.05% NH4OH aqueous solution, running 8 min). The fraction containing the product was concentrated to give the title compound (20 mg; 75% yield) as a solid. 1 H NMR (400MHz, CDCl3) δ6.78(d,J=8.7Hz,1H),6.69(s,1H),6.49(d,J=2.8Hz,1H),6.45(dd,J=8.7,2.9Hz,1H),4.43(s,2H),4.37(hept,J=3.8Hz,1H),4. 29-4.23(m,2H),4.23-4.19(m,2H),3.62-3.51(m,2H),3.50(s,3H),3.23-3 .12(m,2H),2.50(s,3H),2.35(s,3H),2.24-2.07(m,2H),2.02-1.89(m,2H). ES-MS[M+1] + :453.

[0654] Example 14. 7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)piperidin-1-yl)-2-(methoxymethyl)-8-methyl-4H-pyrimido[1,2-b]pyridazin-4-one (Compound 14)

[0655]

[0656] To a solution of 7-chloro-2-(methoxymethyl)-8-methyl-pyrimido[1,2-b]pyridazine-4-one (15 mg) and 4-[(2,2,3,3-tetradeuterated-1,4-benzodioxin-6-yl)oxy]piperidine (30 mg) in NMP (1 mL) was added N,N-diisopropylethylamine (55 μL). The mixture was heated to 180 ° C for 18 hours. The crude product was dissolved in DMSO (1 mL) and purified by reverse phase chromatography using Gilson HPLC (30×100 mm column, 20%-65% ACN / 0.1% TFA aqueous solution, running 10 min). The fraction containing the product was basified with saturated NaHCO3 and extracted with 3:1 chloroform / IPA. The organic extract was passed through a phase separator and concentrated to give the title compound (18 mg, 66% yield) as a solid. 1 H NMR (400MHz, CDCl3) δ7.48(q,J=1.2Hz,1H),6.78(d,J=8.7Hz,1H),6.66(t,J=0.9Hz,1H),6.49(d,J=2.8Hz,1H),6.45(dd,J=8.7,2.9Hz,1H),4.40(d,J =0.9Hz,2H),4.39(hept,J=3.7Hz,1H),3.66-3.56(m,2H),3.49(s,3H),3.3 1-3.20(m,2H),2.43(d,J=1.2Hz,3H),2.18-2.05(m,2H),2.02-1.89(m,2H). ES-MS[M+1] + :443.

[0657] Example 15. 7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)piperidin-1-yl)-3-fluoro-2,8-dimethyl-4H-pyrimido[1,2-b]pyridazin-4-one (Compound 15)

[0658]

[0659] To a solution of 7-chloro-3-fluoro-2,8-dimethyl-pyrimido[1,2-b]pyridazine-4-one (10 mg) and 4-[(2,2,3,3-tetradeuterated-1,4-benzodioxin-6-yl)oxy]piperidine (16 mg) in tert-butanol (0.5 mL) was added N,N-diisopropylethylamine (38 μL). The mixture was heated to 120 ° C for 18 hours. The crude product was dissolved in DMSO (1 mL) and purified by reverse phase chromatography using Gilson HPLC (30×100 mm column, 25%-60% ACN / 0.05% NH4OH aqueous solution, running 8 min). The fractions containing the product were concentrated to give the title compound (10 mg; 54% yield) as a solid. 1 H NMR (400MHz, CDCl3) δ7.38(q,J=1.2Hz,1H),6.72(d,J=8.7Hz,1H),6.43(d,J=2.8Hz,1H),6.39(dd,J=8.7,2.9Hz,1H),4.34(hept,J=3.6Hz,1H), 3.56(ddd,J=12.0,7.6,3.5Hz,2H),3.21(ddd,J=12.5,7.8,3.4Hz,2H),2 .40(d,J=3.5Hz,3H),2.36(s,3H),2.11-2.02(m,2H),1.97-1.78(m,2H). ES-MS[M+1] + :431.

[0660] Example 16. 7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)piperidin-1-yl)-3-fluoro-2,8,9-trimethyl-4H-pyrimido[1,2-b]pyridazin-4-one (Compound 16)

[0661]

[0662] To a solution of 7-chloro-3-fluoro-2,8,9-trimethyl-pyrimido[1,2-b]pyridazine-4-one (10 mg) and 4-[(2,2,3,3-tetradeuterated-1,4-benzodioxin-6-yl)oxy]piperidine (15 mg) in tert-butanol (0.5 mL) was added N,N-diisopropylethylamine (36 μL). The mixture was heated to 120 ° C for 18 hours. The crude product was dissolved in DMSO (1 mL) and purified by reverse phase chromatography using Gilson HPLC (30×100 mm column, 20%-70% ACN / 0.05% NH4OH aqueous solution, running 8 min). The fractions containing the product were concentrated to give the title compound (12 mg; 65% yield) as a solid. 1H NMR (400MHz, MeOD) δ6.73(d,J=8.8Hz,1H),6.51(d,J=2.7Hz,1H),6.48(dd,J=8.7,2.8Hz,1H),4.45(dt,J=7.6,3.9Hz,1H),3.56(ddd,J=11.7,7.2 ,3.5Hz,2H),3.21(ddd,J=12.5,8.2,3.3Hz,2H),2.56(d,J=0.6Hz,3H),2 .50(d,J=3.5Hz,3H),2.41(s,3H),2.25-2.08(m,2H),1.99-1.82(m,2H). ES-MS[M+1] + :445.

[0663] Example 17. 3-Chloro-7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)piperidin-1-yl)-2,8-dimethyl-4H-pyrimido[1,2-b]pyridazin-4-one (Compound 17)

[0664]

[0665] To a solution of 3,7-dichloro-2,8-dimethyl-pyrimido[1,2-b]pyridazine-4-one (10 mg) and 4-[(2,2,3,3-tetradeuterated-1,4-benzodioxin-6-yl)oxy]piperidine (15 mg) in tert-butanol (0.5 mL) was added N,N-diisopropylethylamine (36 μL). The mixture was heated to 120 ° C for 18 hours. The crude product was dissolved in DMSO (1 mL) and purified by reverse phase chromatography using Gilson HPLC (30×100 mm column, 25%-65% ACN / 0.05% NH4OH aqueous solution, running 8 min). The fractions containing the product were concentrated to give the title compound (12.9 mg; 71% yield) as a solid. 1 H NMR (400MHz, CDCl3) δ7.47(d,J=0.6Hz,1H),6.78(d,J=8.7Hz,1H),6.49(d,J=2.8Hz,1H),6.45(dd,J=8.8,2.9Hz,1H),4.40(hep t,J=3.6Hz,1H),3.74-3.57(m,2H),3.33-3.22(m,2H),2.56(s,3H),2.44(d,J=1.2Hz,3H),2.18-2.07(m,2H),2.02-1.89(m,2H). ES-MS[M+1] + :447.

[0666] Example 18. 7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)piperidin-1-yl)-8-(methoxymethyl)-9-methyl-4H-pyrimido[1,2-b]pyridazin-4-one (Compound 18)

[0667]

[0668] To a solution of 7-chloro-8-(methoxymethyl)-9-methyl-pyrimido[1,2-b]pyridazine-4-one (15 mg) and 4-[(2,2,3,3-tetradeuterated-1,4-benzodioxin-6-yl)oxy]piperidine (30 mg) in tert-butanol (1 mL) was added N,N-diisopropylethylamine (55 μL). The mixture was heated to 120 ° C for 18 hours. The crude product was dissolved in DMSO (1 mL) and purified by reverse phase chromatography using Gilson HPLC (30×100 mm column, 20%-65% ACN / 0.1% TFA aqueous solution, running 8 min). The fraction containing the product was basified with saturated NaHCO3 and extracted with 4:1 chloroform / IPA. The organic extract was passed through a phase separator and concentrated to give the title compound (11.4 mg, 41% yield) as a solid. 1 H NMR (400MHz, CDCl3) δ8.15(d,J=6.3Hz,1H),6.78(d,J=8.7Hz,1H),6.58(d,J=6.3Hz,1H ),6.49(d,J=2.8Hz,1H),6.45(dd,J=8.7,2.9Hz,1H),4.42(s,2H),4.37(hept,J=3.7Hz ,1H),3.67(ddd,J=11.3,6.9,3.6Hz,2H),3.48(s,3H),3.25(ddd,J=12.6,8.5,3.2Hz,2 H), 2.60 (s, 3H), 2.16 (ddt, J = 13.4, 6.9, 3.5Hz, 2H), 1.95 (dtd, J = 12.4, 8.0, 3.5Hz, 2H). ES-MS[M+1] + :443.

[0669] Example 19. 7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)piperidin-1-yl)-9-(methoxymethyl)-8-methyl-4H-pyrimido[1,2-b]pyridazin-4-one (Compound 19)

[0670]

[0671] To a solution of 7-chloro-9-(methoxymethyl)-8-methyl-pyrimido[1,2-b]pyridazine-4-one (15 mg) and 4-[(2,2,3,3-tetradeuterated-1,4-benzodioxin-6-yl)oxy]piperidine (30 mg) in tert-butanol (1 mL) was added N,N-diisopropylethylamine (55 μL). The mixture was heated to 120 ° C for 18 hours. The crude product was dissolved in DMSO (1 mL) and purified by reverse phase chromatography using Gilson HPLC (30×100 mm column, 15%-55% ACN / 0.1% TFA aqueous solution, running 8 min). The fraction containing the product was basified with saturated NaHCO3 and extracted with 4:1 chloroform / IPA. The organic extract was passed through a phase separator and concentrated to give the title compound (6 mg, 22% yield) as a solid. 1 H NMR (400MHz, CDCl3) δ8.14(d,J=6.3Hz,1H),6.78(d,J=8.7Hz,1H),6.54(d,J=6.3H z,1H),6.49(d,J=2.8Hz,1H),6.45(dd,J=8.7,2.9Hz,1H),4.91(s,2H),4.38(hept, J=3.7Hz,1H),3.58(ddd,J=11.8,7.4,3.5Hz,2H),3.48(s,3H),3.22(ddd,J=12.4,8 .0,3.3Hz,2H),2.48(s,3H),2.18-2.08(m,2H),1.96(dtd,J=11.2,7.6,3.4Hz,2H). ES-MS[M+1] + :443.

[0672] Example 20. 9-Cyclopropyl-7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)piperidin-1-yl)-8-methyl-4H-pyrimido[1,2-b]pyridazin-4-one (Compound 20)

[0673]

[0674] To a solution of 7-chloro-9-cyclopropyl-8-methyl-pyrimido[1,2-b]pyridazine-4-one (15 mg) and 4-(2,3-dihydro-1,4-benzodioxin-6-yloxy)piperidine (43 mg) in tert-butanol (0.5 mL) was added N,N-diisopropylethylamine (55 μL). The mixture was heated to 120 ° C for 18 hours. The crude product was dissolved in DMSO (1 mL) and purified by reverse phase chromatography using Gilson HPLC (30×100 mm column, 20%-60% ACN / 0.05% NH4OH aqueous solution, running 8 min). The fraction containing the product was concentrated to give the title compound (16 mg; 57% yield) as a solid. 1 H NMR (400MHz, CDCl3) δ8.14(d,J=6.3Hz,1H),6.78(d,J=8.7Hz,1H),6.52(d,J=6.3H z,1H),6.49(d,J=2.8Hz,1H),6.45(dd,J=8.7,2.9Hz,1H),4.37(tt,J=3.8Hz,1H), 4.27-4.24(m,2H),4.24-4.18(m,2H),3.63-3.51(m,2H),3.29-3.10(m,2H),2.50( s,3H),2.18-2.07(m,2H),2.01-1.87(m,3H),1.34-1.18(m,2H),1.14-1.07(m,2H). ES-MS[M+1] + :435.

[0675] Example 21. 8-Cyclopropyl-7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)piperidin-1-yl)-9-methyl-4H-pyrimido[1,2-b]pyridazin-4-one (Compound 21)

[0676]

[0677] To a solution of 7-chloro-8-cyclopropyl-9-methyl-pyrimido[1,2-b]pyridazine-4-one (15 mg) and 4-[(2,2,3,3-tetradeuterated-1,4-benzodioxin-6-yl)oxy]piperidine (30 mg) in tert-butanol (0.5 mL) was added N,N-diisopropylethylamine (55 μL). The mixture was heated to 120 ° C for 18 hours. The crude product was dissolved in DMSO (1 mL) and purified by reverse phase chromatography using Gilson HPLC (30×100 mm column, 25%-65% ACN / 0.05% NH4OH aqueous solution, running 8 min). The fractions containing the product were concentrated to give the title compound (1.8 mg; 7% yield) as a solid.1 H NMR (400MHz, CDCl3) δ8.13(d,J=8.0Hz,1H),6.78(d,J=8.0Hz,1H),6.53(d,J =8.0Hz,1H),6.49(d,J=4.0Hz,1H),6.45(dd,J=8.0,4.0Hz,1H),4.38-4.35( m,1H),3.85-3.79(m,2H),3.40-3.31(m,2H),2.66(s,3H),2.20-2.12(m,2H) ,2.01-1.93(m,2H),1.88-1.84(m,1H),1.30-1.17(m,2H),0.81-0.73(m,2H). ES-MS[M+1] + :435.

[0678] Example 22. 7-((2R,4S)-4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl)oxy)-2-methylpiperidin-1-yl)-8-methyl-4H-pyrimido[1,2-b]pyridazin-4-one (Compound 108)

[0679]

[0680] To a solution of (2R,4S)-4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl)oxy)-2-methylpiperidine (50 mg) in NMP (1 mL) was added 7-chloro-8-methyl-4H-pyrimido[1,2-b]pyridazin-4-one (43 mg) and DIEA (156 mg), and the mixture was microwaved at 180 ° C for 2 hours. The reaction mixture was diluted with EtOAc (10 mL) and extracted with H2O (10 mL×3). The combined organic layers were washed with brine (10 mL), dried over Na2SO4, filtered and concentrated. The residue was purified by basic preparative HPLC to give 7-((2R,4S)-4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl)oxy)-2-methylpiperidin-1-yl)-8-methyl-4H-pyrimido[1,2-b]pyridazin-4-one (16 mg). 1H NMR(400MHz, CDCl3)δ8.11(d,J=6.4Hz,1H),7.51(s,1H),6.78(d,J=8.8Hz,1H), 6.54(d,J=6.4Hz,1H),6.51-6.42(m,2H),4.62-4.47(m,1H),4.29-4.20(m,4H),4 .19-4.11(m,1H),3.62-3.56(m,1H),3.40-3.33(m,1H),2.45(s,3H),2.18-2.15 (m,1H),2.07-1.99(m,2H),1.95-1.82(m,1H),1.27(d,J=6.4Hz,3H); ES-MS[M+1] + :409.1.

[0681] Example 23. 7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl)oxy)piperidin-1-yl)-9-methoxy-8-methyl-4H-pyrimido[1,2-b]pyridazin-4-one (Compound 126)

[0682]

[0683] To a solution of 9-bromo-7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl)oxy)piperidin-1-yl)-8-methyl-4H-pyrimido[1,2-b]pyridazin-4-one (300 mg) in MeOH (5 mL) was added NaOMe (137 mg), and the resulting mixture was stirred at 60° C. for 6 hours. H2O (5 mL) was added to the reaction and extracted with EtOAc (5 mL×5). The combined organic layers were dried over Na2SO4, filtered, and concentrated. The crude compound was purified by preparative thin layer chromatography (SiO2, ethyl acetate:petroleum ether = 1:0) to give 7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl)oxy)piperidin-1-yl)-9-methoxy-8-methyl-4H-pyrimido[1,2-b]pyridazin-4-one (55 mg). 1H NMR (400MHz, CDCl3) δ8.15(d,J=6.4Hz,1H),6.79(d,J=8.4Hz,1H),6.57(d,J=6.4Hz,1H),6.50(d,J=2.4Hz,1H),6.46(dd,J=2.8,8.8Hz,1H),4.41-3.3 6(m,1H),4.28-4.25(m,2H),4.24-4.21(m,2H),4.18(s,3H),3.64-3.55(m, 2H),3.27-3.18(m,2H),2.33(s,3H),2.18-2.09(m,2H),2.01-1.91(m,2H). ES-MS[M+1] + :425.2.

[0684] Example 24. 9-(azetidin-1-yl)-7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl)oxy)piperidin-1-yl)-8-methyl-4H-pyrimido[1,2-b]pyridazin-4-one (Compound 127)

[0685]

[0686] A mixture of 9-bromo-7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl)oxy)piperidin-1-yl)-8-methyl-4H-pyrimido[1,2-b]pyridazin-4-one (200 mg), azetidine hydrochloride (198 mg), Cs2CO3 (1.1 g), BrettPhos Pd G3 (77 mg) and BRETTPHOS (45 mg) in toluene (5 mL) was degassed and purged with N2 three times, and then the mixture was stirred at 100° C. for 16 hours under N2 atmosphere. The reaction mixture was concentrated and the residue was purified by silica gel flash chromatography ( ; 24g Silica flash column, 0%-100% ethyl acetate / petroleum ether gradient eluent, 30mL / min) purify to give crude product.The crude product is purified by recrystallization from EtOAc (3mL) at 80 DEG C. Recrystallization again with DCM (5mL) and EtOAc (15mL) provides 9- (azetidine -1- bases) -7- (4- ((2,3- dihydrobenzo [b] [1,4] dioxin -6- bases) oxygen base) piperidine -1- bases) -8- methyl -4H- pyrimido [1,2-b] pyridazine -4- ketone (50mg). 1H NMR (400MHz, CDCl3) δ7.98(d,J=6.0Hz,1H),6.78(d,J=8.8Hz,1H),6.50-6.44(m,3H),4.68(t,J=8.0Hz,4H),4.39-4.29(m,1H),4.28-4.2 4(m,2H),4.24-4.18(m,2H),3.62-3.52(m,2H),3.21-3.12(m,2H),2.40-2.32(m,2H),2.20(s,3H),2.15-2.06(m,2H),1.96-1.85(m,2H). ES-MS[M+1] + :450.0.

[0687] Example 25. 7-((2R,4S)-4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)-2-methylpiperidin-1-yl)-8-methyl-4H-pyrimido[1,2-b]pyridazin-4-one (Compound 129)

[0688]

[0689] A mixture of 7-chloro-8-methyl-4H-pyrimido[1,2-b]pyridazin-4-one (2 g), (2R,4S)-4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)-2-methylpiperidine (2.59 g) and CsF (1.86 g) in DMSO (20 mL) was stirred at 80° C. for 2 hours. The mixture was diluted with H2O (20 mL) and extracted with EtOAc (40 mL×5). The combined organic layers were washed with brine (50 mL), dried over Na2SO4 and concentrated. The residue was purified by Combi Flash (silica gel, from 0% to 100%, ethyl acetate in petroleum ether), followed by preparative HPLC (instrument: Gilson GX-215 liquid handler, SHIMADZU LC-20AP, SHIMADZU SPD-20A; column: Xtimate C18 150×40 mm×5 μm; mobile phase A: water (0.05% NH 3 H 2 O); mobile phase B: MeCN; gradient: from 35% to 55% in 9 min. B was then maintained at 100% for 5 min; flow rate (ml / min): 60; column temperature: 30°C; wavelength: 220 nm, 254 nm) to give 7-((2R,4S)-4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)-2-methylpiperidin-1-yl)-8-methyl-4H-pyrimido[1,2-b]pyridazin-4-one (1.4 g).1 H NMR (400MHz, CDCl3) δ8.10(d,J=6.4Hz,1H),7.50(d,J=1.2Hz,1H),6.77(d,J=8.8Hz ,1H),6.53(d,J=6.4Hz,1H),6.48(d,J=2.8Hz,1H),6.44(d,J=8.8Hz,2.8Hz,1H),4. 56-4.48(m,1H),4.18-4.11(m,1H),3.61-3.55(m,1H),3.39-3.32(m,1H),2.44(s,3 H),2.21-2.12(m,1H),2.07-1.97(m,2H),1.93-1.84(m,1H),1.27(d,J=6.8Hz,3H). ES-MS[M+1] + :413.0. SFC:t R =3.62min, ee=99.8%, [α] 20 D =+104.0 (c=1.2 g / 100 mL, DCM). SFC method: Instrument: Waters UPCC with PAD detector; Column: Chiralcel OJ-3100×4.6 mm ID, 3 μm; Mobile phase: A: CO2 B: Ethanol (0.05% DEA); Gradient: 5% to 40% B in 4 min and hold at 40% for 2.5 min, then 5% B for 1.5 min; Flow rate: 2.8 mL / min; Column temperature: 35°C; ABPR: 1500 psi; Run time: 8 min; Wavelength: 220 nm.

[0690] The compounds shown in Table 1 were prepared in a similar manner using appropriate starting materials.

[0691] Table 1

[0692]

[0693]

[0694]

[0695]

[0696]

[0697]

[0698]

[0699]

[0700]

[0701]

[0702]

[0703]

[0704]

[0705]

[0706]

[0707]

[0708]

[0709]

[0710]

[0711]

[0712]

[0713]

[0714]

[0715]

[0716]

[0717]

[0718]

[0719]

[0720] c. Biological activity

[0721] A. Cell lines expressing muscarinic acetylcholine receptors

[0722] Human and rat M4 cDNA were stained with chimeric G protein G using Lipofectamine 2000. qi5The hM4-G was transfected into Chinese hamster ovary (CHO-K1) cells purchased from the American Type Culture Collection. qi5 Cells were grown in Ham's F-12 medium containing 10% heat-inactivated fetal bovine serum (FBS), 20 mM HEPES, 50 μg / mL G418 sulfate, and 500 μg / mL hygromycin B. qi5 Cells were grown in DMEM containing 10% heat-inactivated FBS, 20 mM HEPES, 400 μg / mL G418 sulfate, and 500 μg / mL hygromycin B.

[0723] B. Cell-based functional assays of muscarinic acetylcholine receptor activity

[0724] For high-throughput measurement of agonist-induced intracellular calcium increases, CHO-K1 cells stably expressing muscarinic receptors were plated in Griner 384-well black-walled tissue culture (TC) treated clear bottom plates (VWR) at 15,000 cells / 20 μL / well in growth medium lacking G418 and hygromycin. The cells were incubated overnight at 37°C and 5% CO2. The next day, the cells were washed with assay buffer using ELX 405 (BioTek); the final volume was then pipetted to 20 μL. Then, 20 μL of 2.3 μM Fluo-4 / acetoxymethyl ester stock (Invitrogen, Carlsbad, CA) (prepared as 2.3 mM stock in DMSO and mixed with 10% (w / v) Pluronic F-127 at a 1:1 ratio and diluted in assay buffer) was added to the wells, and the cell plates were incubated at 37°C and 5% CO2 for 50 minutes. The dye was removed by washing with ELX 405 and the final volume was aspirated to 20 μL. The compound master was set up in 100% DMSO in an 11-point concentration-response curve (CRC) format (1:3 dilution) using a BRAVO liquid handler (Agilent) at a starting concentration of 10 mM. The test compound CRC was then transferred to a daughter plate (240 nL) using an Echo acoustic plate reformatter (Labcyte, Sunnyvale, CA) and then diluted to 2× stock in assay buffer (40 μL) using a Thermo Fisher Combi (Thermo Fisher Scientific, Waltham, MA).

[0725] Calcium flux was measured using a functional drug screening system (FDSS) 6000 or 7000 (Hamamatsu Corporation, Tokyo, Japan) as an increase in the fluorescence static ratio. Compounds were applied to cells (20 μL, 2X) in 2-4 seconds using the automated system of the FDSS, and data were collected at 1 Hz. At 144 seconds, 10 μL EC 20 concentration of muscarinic receptor agonist acetylcholine (5X), followed by the addition of 12 μL EC 80 concentration of acetylcholine (5X). Agonist activity was analyzed as a concentration-dependent increase in calcium mobilization after compound addition. Positive allosteric modulator activity was analyzed as EC 20 The antagonist activity was analyzed as EC 80 Concentration-dependent decrease in acetylcholine response. Concentration-response curves were generated using a four-parameter logistic equation in XLFit curve fitting software (IDBS, Bridgewater, NJ) for Excel (Microsoft, Redmond, WA) or Prism (GraphPad Software, Inc., San Diego, CA).

[0726] The above assay was also operated in a second mode, wherein an appropriate fixed concentration of a compound of the invention was added to the cells approximately 3 seconds after the fluorescence baseline was established, and the response in these cells was measured. After 140 s, an appropriate concentration of an agonist was added, and the calcium response (maximum-local minimum response) was measured. The EC of the agonist in the presence of the test compound was determined by nonlinear curve fitting. 50 The EC of the agonist decreased with increasing concentration of the compound of the present invention. 50 The value (leftward shift of the agonist concentration-response curve) indicates the extent of positive allosteric modulation of muscarinic activity at a given concentration of a compound of the invention. The EC of an agonist that increases with increasing concentration of a compound of the invention is 50 The value (rightward shift of the agonist concentration-response curve) indicates the extent of muscarinic antagonism at a given concentration of the compound of the invention. The second approach also indicates whether the compound of the invention also affects the maximal response of the muscarinic receptor to the agonist.

[0727] d. Activity of compounds in mAChR M4 cell-based assay

[0728] Compounds were synthesized as described above. Activity (EC 50 and E 最大), and the data are shown in Table 2. The compound numbers correspond to the compound numbers used in Examples 1 to 11 and Table 1.

[0729] Table 2.

[0730]

[0731]

[0732]

[0733] *% ACh Max at 30 μM.

[0734] It should be understood that the above detailed description and accompanying examples are illustrative only and should not be taken as limiting the scope of the present invention, which is defined solely by the appended claims and their equivalents.

[0735] Various changes and modifications of the disclosed embodiments will be apparent to those skilled in the art. Such changes and modifications may be made without departing from the spirit and scope of the present invention, including but not limited to those changes and modifications related to the chemical structures, substituents, derivatives, intermediates, syntheses, compositions, formulations or methods of use of the present invention.

Claims

1. Compounds of formula (IA) or a pharmaceutically acceptable salt thereof, wherein X is CR 5a or N; R 1 is selected from hydrogen, C1-C4-alkyl, C1-C4-haloalkyl, C3-C6-cycloalkyl, -L 1 -C3-C6-cycloalkyl and -L 1 OR a ; R 2 is selected from hydrogen, C1-C4-alkyl, C3-C6-cycloalkyl, -L 2 OR b , OR b 、NHR b and N(R b )2; R 3 is selected from C1-C4-alkyl, hydrogen, C3-C6-cycloalkyl and -L 3 OR c ; R 4 independently selected from C1-C4-alkyl and halogen; Two R's 5c Together with the carbon atoms to which they are attached, they form a 5- to 8-membered fused monocyclic heterocyclic ring containing 2 or 1 heteroatom independently selected from O, N and S, the fused monocyclic heterocyclic ring being optionally substituted by 1-4 R 6 replace; or Each R 5c are independently selected from hydrogen, C1-C4-alkyl, C1-C4-haloalkyl, OR e and halogens; R 5a is selected from hydrogen, C1-C4-alkyl and halogen; Each R 6 independently selected from oxo and C1-C4-alkyl; R 7 is selected from hydrogen, C1-C4-alkyl, C3-C6-cycloalkyl, halogen and N(R b )2; o is 0 or 1; n is 0; L 1 , L 2 and L 3 are each independently -CH2-; and R a , R b , R c and R e are each independently C1-C4-alkyl, wherein two R b Together with the nitrogen atom to which they are attached they optionally form an azetidinyl group.

2. The compound according to claim 1 or a pharmaceutically acceptable salt thereof, wherein two R 5c Together with the carbon atoms to which they are attached they form the 5- to 8-membered fused monocyclic heterocyclic ring.

3. The compound according to claim 2 or a pharmaceutically acceptable salt thereof, wherein 5c The 5- to 8-membered fused monocyclic heterocyclic ring formed contains 2 or 1 heteroatom independently selected from O and N, and is optionally substituted by 1-2 R 6 replace.

4. The compound of claim 2 or a pharmaceutically acceptable salt thereof, which has formula (IB), in G 1 yes and Each q is independently 0, 1, 2, 3 or 4.

5. The compound according to claim 4 or a pharmaceutically acceptable salt thereof, wherein G 1 yes 6. The compound according to claim 5 or a pharmaceutically acceptable salt thereof, wherein G 1 yes 7. The compound according to claim 4 or a pharmaceutically acceptable salt thereof, which has formula (Ia), 8. The compound according to claim 4 or a pharmaceutically acceptable salt thereof, which has formula (Ib), 9. The compound according to claim 1 or a pharmaceutically acceptable salt thereof, wherein each R 5c are independently selected from hydrogen, C1-C4-alkyl, C1-C4-haloalkyl, OR e and halogens.

10. The compound of claim 9 having formula (IB) or a pharmaceutically acceptable salt thereof, in G 1 yes 11. The compound according to claim 10 or a pharmaceutically acceptable salt thereof, wherein G 1 yes 12. The compound or pharmaceutically acceptable salt thereof according to any one of claims 1 to 11, wherein R 3 It is C1-C4-alkyl.

13. The compound according to claim 12 or a pharmaceutically acceptable salt thereof, wherein R 3 It's methyl.

14. The compound or pharmaceutically acceptable salt thereof according to any one of claims 1 to 11, wherein R 1 It is C1-C4-alkyl.

15. The compound or pharmaceutically acceptable salt thereof according to any one of claims 1 to 11, wherein R 1 It's hydrogen.

16. The compound or pharmaceutically acceptable salt thereof according to any one of claims 1 to 11, wherein R 1 It is a C1-C4-fluoroalkyl group.

17. The compound according to claim 16 or a pharmaceutically acceptable salt thereof, wherein R 1 It is trifluoromethyl.

18. The compound according to claim 16 or a pharmaceutically acceptable salt thereof, wherein R 1 It is difluoromethyl.

19. The compound or pharmaceutically acceptable salt thereof according to any one of claims 1 to 11, wherein R 2 It is C1-C4-alkyl.

20. The compound or pharmaceutically acceptable salt thereof according to any one of claims 1 to 11, wherein R 2 It's hydrogen.

21. The compound of claim 19 or a pharmaceutically acceptable salt thereof, wherein R 2 It's methyl.

22. The compound of any one of claims 1-11 or a pharmaceutically acceptable salt thereof, wherein o is 0.

23. The compound or pharmaceutically acceptable salt thereof of any one of claims 1-11, wherein o is 1.

24. A compound or a pharmaceutically acceptable salt thereof as described in any one of claims 1 to 11, wherein R 7 is selected from the group consisting of hydrogen, methyl, cyclopropyl, fluoro, chloro and azetidin-1-yl.

25. The compound of claim 24 or a pharmaceutically acceptable salt thereof, wherein R 7 It's hydrogen.

26. A compound or a pharmaceutically acceptable salt thereof as claimed in any one of claims 1 to 4 or 7 to 9, wherein X is CR 5a .

27. The compound of any one of claims 1 to 4 or 7 to 9, or a pharmaceutically acceptable salt thereof, wherein X is N.

28. The compound of claim 26 or a pharmaceutically acceptable salt thereof, wherein R 5a It's hydrogen.

29. The compound of claim 1, selected from the group consisting of: 7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)piperidin-1-yl)-8-methyl-4H-pyrimido[1,2-b]pyridazin-4-one; 7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)piperidin-1-yl)-9-methyl-4H-pyrimido[1,2-b]pyridazin-4-one; 3-Chloro-7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)piperidin-1-yl)-8-methyl-4H-pyrimido[1,2-b]pyridazin-4-one; 8-methyl-7-(4-((6-methylpyridin-3-yl)oxy)piperidin-1-yl)-4H-pyrimido[1,2-b]pyridazin-4-one; 7-(4-(4-fluorophenoxy)piperidin-1-yl)-8-methyl-4H-pyrimido[1,2-b]pyridazin-4-one; 7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl)oxy)piperidin-1-yl)-8-methyl-4H-pyrimido[1,2-b]pyridazin-4-one; 3-Chloro-8-methyl-7-(4-((8-methyl-2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)piperidin-1-yl)-4H-pyrimido[1,2-b]pyridazin-4-one; 8-methyl-7-(4-((8-methyl-2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)piperidin-1-yl)-4H-pyrimido[1,2-b]pyridazin-4-one; 7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)piperidin-1-yl)-3-fluoro-2,8-dimethyl-4H-pyrimido[1,2-b]pyridazin-4-one; 7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)piperidin-1-yl)-3,8-dimethyl-4H-pyrimido[1,2-b]pyridazin-4-one; 3-cyclopropyl-7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)piperidin-1-yl)-8-methyl-4H-pyrimido[1,2-b]pyridazin-4-one; 3-Chloro-7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl)oxy)piperidin-1-yl)-8-methyl-4H-pyrimido[1,2-b]pyridazin-4-one; 7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)piperidin-1-yl)-2,8-dimethyl-4H-pyrimido[1,2-b]pyridazin-4-one; 7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)piperidin-1-yl)-8-methyl-2-(trifluoromethyl)-4H-pyrimido[1,2-b]pyridazin-4-one; 7-((3R,4R)-4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl)oxy)-3-fluoropiperidin-1-yl)-8-methyl-4H-pyrimido[1,2-b]pyridazin-4-one; 7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)piperidin-1-yl)-3-fluoro-2,8,9-trimethyl-4H-pyrimido[1,2-b]pyridazin-4-one; 7-((3S,4R)-4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl)oxy)-3-fluoropiperidin-1-yl)-2,8-dimethyl-4H-pyrimido[1,2-b]pyridazin-4-one; 7-((3S,4R)-4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl)oxy)-3-fluoropiperidin-1-yl)-8-methyl-4H-pyrimido[1,2-b]pyridazin-4-one; 3-(azetidin-1-yl)-7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)piperidin-1-yl)-8-methyl-4H-pyrimido[1,2-b]pyridazin-4-one; 7-((3R,4R)-4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl)oxy)-3-fluoropiperidin-1-yl)-3-fluoro-2,8-dimethyl-4H-pyrimido[1,2-b]pyridazin-4-one; 7-((3S,4S)-4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl)oxy)-3-fluoropiperidin-1-yl)-3-fluoro-2,8-dimethyl-4H-pyrimido[1,2-b]pyridazin-4-one; 7-((3S,4S)-4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)-3-fluoropiperidin-1-yl)-3-fluoro-2,8-dimethyl-4H-pyrimido[1,2-b]pyridazin-4-one; 7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)piperidin-1-yl-4-d)-8-methyl-4H-pyrimido[1,2-b]pyridazin-4-one; 7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)piperidin-1-yl-4-d)-2,8-dimethyl-4H-pyrimido[1,2-b]pyridazin-4-one; 7-((3R,4R)-4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)-3-fluoropiperidin-1-yl)-8-methyl-4H-pyrimido[1,2-b]pyridazin-4-one; 7-((3S,4S)-4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)-3-fluoropiperidin-1-yl)-8-methyl-4H-pyrimido[1,2-b]pyridazin-4-one; 7-((3R,4S)-4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl)oxy)-3-fluoropiperidin-1-yl)-8-methyl-4H-pyrimido[1,2-b]pyridazin-4-one; 7-((3R,4S)-4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl)oxy)-3-fluoropiperidin-1-yl)-2,8-dimethyl-4H-pyrimido[1,2-b]pyridazin-4-one; 7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)piperidin-1-yl)-2,3,8-trimethyl-4H-pyrimido[1,2-b]pyridazin-4-one; 2-(Difluoromethyl)-7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)piperidin-1-yl)-8-methyl-4H-pyrimido[1,2-b]pyridazin-4-one; 2-Cyclopropyl-7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)piperidin-1-yl)-8-methyl-4H-pyrimido[1,2-b]pyridazin-4-one; 7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)piperidin-1-yl-4-d)-8-methyl-2-(trifluoromethyl)-4H-pyrimido[1,2-b]pyridazin-4-one; 7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)piperidin-1-yl-4-d)-3-fluoro-2,8-dimethyl-4H-pyrimido[1,2-b]pyridazin-4-one; 7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)piperidin-1-yl-4-d)-2,3,8-trimethyl-4H-pyrimido[1,2-b]pyridazin-4-one; 3-Chloro-7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)piperidin-1-yl)-2,8-dimethyl-4H-pyrimido[1,2-b]pyridazin-4-one; 7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl)oxy)piperidin-1-yl-4-d)-8-methyl-4H-pyrimido[1,2-b]pyridazin-4-one; 7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl)oxy)piperidin-1-yl-4-d)-8-methyl-2-(trifluoromethyl)-4H-pyrimido[1,2-b]pyridazin-4-one; 7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl)oxy)piperidin-1-yl-4-d)-3-fluoro-2,8-dimethyl-4H-pyrimido[1,2-b]pyridazin-4-one; 7-((3R,4R)-4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)-3-fluoropiperidin-1-yl)-2,8-dimethyl-4H-pyrimido[1,2-b]pyridazin-4-one; 2,8-Dimethyl-7-(4-((6-methylpyridin-3-yl)oxy)piperidin-1-yl)-4H-pyrimido[1,2-b]pyridazin-4-one; 7-((3R,4R)-4-(Benzo[d][1,3]dioxol-5-yloxy)-3-fluoropiperidin-1-yl)-8-methyl-4H-pyrimido[1,2-b]pyridazin-4-one; 7-((3R,4R)-3-fluoro-4-(isochroman-6-yloxy)piperidin-1-yl)-8-methyl-4H-pyrimido[1,2-b]pyridazin-4-one; 7-((3R,4R)-4-((3,4-dihydro-2H-benzo[b][1,4]dioxepin-7-yl)oxy)-3-fluoropiperidin-1-yl)-8-methyl-4H-pyrimido[1,2-b]pyridazin-4-one; 7-((3R,4R)-4-((2,3-dihydrobenzofuran-5-yl)oxy)-3-fluoropiperidin-1-yl)-8-methyl-4H-pyrimido[1,2-b]pyridazin-4-one; 7-((3R,4R)-4-(chroman-7-yloxy)-3-fluoropiperidin-1-yl)-8-methyl-4H-pyrimido[1,2-b]pyridazin-4-one; 7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)piperidin-1-yl)-2,8,9-trimethyl-4H-pyrimido[1,2-b]pyridazin-4-one; 7-(4-(Benzo[d][1,3]dioxol-5-yloxy)piperidin-1-yl-4-d)-2,8-dimethyl-4H-pyrimido[1,2-b]pyridazin-4-one; 7-(4-((3,4-dihydro-2H-benzo[b][1,4]dioxepin-7-yl)oxy)piperidin-1-yl-4-d)-2,8-dimethyl-4H-pyrimido[1,2-b]pyridazin-4-one; 7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl)oxy)piperidin-1-yl-4-d)-2,3,8-trimethyl-4H-pyrimido[1,2-b]pyridazin-4-one; 2-(Difluoromethyl)-7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl)oxy)piperidin-1-yl-4-d)-8-methyl-4H-pyrimido[1,2-b]pyridazin-4-one; 7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl)oxy)piperidin-1-yl-4-d)-2,8-dimethyl-4H-pyrimido[1,2-b]pyridazin-4-one; 7-(4-(Benzo[d][1,3]dioxol-5-yloxy)piperidin-1-yl-4-d)-8-methyl-2-(trifluoromethyl)-4H-pyrimido[1,2-b]pyridazin-4-one; 7-(4-((3,4-dihydro-2H-benzo[b][1,4]dioxepin-7-yl)oxy)piperidin-1-yl-4-d)-8-methyl-2-(trifluoromethyl)-4H-pyrimido[1,2-b]pyridazin-4-one; 2-(Difluoromethyl)-7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)piperidin-1-yl-4-d)-8-methyl-4H-pyrimido[1,2-b]pyridazin-4-one; 7-(4-(Benzo[d][1,3]dioxol-5-yloxy)piperidin-1-yl-4-d)-2-(difluoromethyl)-8-methyl-4H-pyrimido[1,2-b]pyridazin-4-one; 2-(Difluoromethyl)-7-(4-((3,4-dihydro-2H-benzo[b][1,4]dioxepin-7-yl)oxy)piperidin-1-yl-4-d)-8-methyl-4H-pyrimido[1,2-b]pyridazin-4-one; 7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)piperidin-1-yl)-8,9-dimethyl-2-(trifluoromethyl)-4H-pyrimido[1,2-b]pyridazin-4-one; 2-(Difluoromethyl)-7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)piperidin-1-yl)-8,9-dimethyl-4H-pyrimido[1,2-b]pyridazin-4-one; 7-(4-(Benzo[d][1,3]dioxol-5-yloxy)piperidin-1-yl-4-d)-8-methyl-4H-pyrimido[1,2-b]pyridazin-4-one; 7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)piperidin-1-yl-4-d)-8,9-dimethyl-4H-pyrimido[1,2-b]pyridazin-4-one; 7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl)oxy)piperidin-1-yl-4-d)-8,9-dimethyl-4H-pyrimido[1,2-b]pyridazin-4-one; 7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl)oxy)piperidin-1-yl)-8,9-dimethyl-4H-pyrimido[1,2-b]pyridazin-4-one; 7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)piperidin-1-yl)-8,9-dimethyl-4H-pyrimido[1,2-b]pyridazin-4-one; 2,8-Dimethyl-7-(4-((1-oxo-1,2,3,4-tetrahydroisoquinolin-7-yl)oxy)piperidin-1-yl)-4H-pyrimido[1,2-b]pyridazin-4-one; 2,8-Dimethyl-7-(4-((2-methyl-1-oxo-1,2,3,4-tetrahydroisoquinolin-7-yl)oxy)piperidin-1-yl)-4H-pyrimido[1,2-b]pyridazin-4-one; 2,8-Dimethyl-7-(4-((2-methyl-1-oxo-1,2,3,4-tetrahydroisoquinolin-6-yl)oxy)piperidin-1-yl)-4H-pyrimido[1,2-b]pyridazin-4-one; 7-(4-((6-methoxy-5-methylpyridin-3-yl)oxy)piperidin-1-yl)-8-methyl-4H-pyrimido[1,2-b]pyridazin-4-one; 8,9-dimethyl-7-(4-(p-tolyloxy)piperidin-1-yl)-4H-pyrimido[1,2-b]pyridazin-4-one; 2-(Difluoromethyl)-8,9-dimethyl-7-(4-((6-methylpyridin-3-yl)oxy)piperidin-1-yl)-4H-pyrimido[1,2-b]pyridazin-4-one; 7-(4-(4-chlorophenoxy)piperidin-1-yl)-2-(difluoromethyl)-8,9-dimethyl-4H-pyrimido[1,2-b]pyridazin-4-one; 8,9-dimethyl-7-(4-(4-(trifluoromethyl)phenoxy)piperidin-1-yl)-4H-pyrimido[1,2-b]pyridazin-4-one; 2-(Difluoromethyl)-8,9-dimethyl-7-(4-(4-(trifluoromethyl)phenoxy)piperidin-1-yl)-4H-pyrimido[1,2-b]pyridazin-4-one; 2,8-Dimethyl-7-(4-((1-oxo-1,2,3,4-tetrahydroisoquinolin-6-yl)oxy)piperidin-1-yl)-4H-pyrimido[1,2-b]pyridazin-4-one; 7-(4-(4-fluorophenoxy)piperidin-1-yl)-8,9-dimethyl-4H-pyrimido[1,2-b]pyridazin-4-one; 2-(Difluoromethyl)-7-(4-(4-fluorophenoxy)piperidin-1-yl)-8,9-dimethyl-4H-pyrimido[1,2-b]pyridazin-4-one; 2-(Difluoromethyl)-8,9-dimethyl-7-(4-(p-tolyloxy)piperidin-1-yl)-4H-pyrimido[1,2-b]pyridazin-4-one; 8,9-dimethyl-7-(4-((6-methylpyridin-3-yl)oxy)piperidin-1-yl)-4H-pyrimido[1,2-b]pyridazin-4-one; 8,9-dimethyl-7-(4-((6-methylpyridin-3-yl)oxy)piperidin-1-yl)-4H-pyrimido[1,2-b]pyridazin-4-one; 7-(4-(3-fluoro-4-methylphenoxy)piperidin-1-yl)-8-methyl-4H-pyrimido[1,2-b]pyridazin-4-one; 7-(4-(4-ethylphenoxy)piperidin-1-yl)-8-methyl-4H-pyrimido[1,2-b]pyridazin-4-one; 7-(4-(4-isopropylphenoxy)piperidin-1-yl)-8-methyl-4H-pyrimido[1,2-b]pyridazin-4-one; 8-methyl-7-(4-(4-propylphenoxy)piperidin-1-yl)-4H-pyrimido[1,2-b]pyridazin-4-one; 8-methyl-7-(4-(p-tolyloxy)piperidin-1-yl-4-d)-4H-pyrimido[1,2-b]pyridazin-4-one; 7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl)oxy)piperidin-1-yl)-2-ethyl-8,9-dimethyl-4H-pyrimido[1,2-b]pyridazin-4-one; 7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl)oxy)piperidin-1-yl)-2-isopropyl-8,9-dimethyl-4H-pyrimido[1,2-b]pyridazin-4-one; 7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl)oxy)piperidin-1-yl)-2-(methoxymethyl)-8,9-dimethyl-4H-pyrimido[1,2-b]pyridazin-4-one; 8-cyclopropyl-7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl)oxy)piperidin-1-yl)-9-methyl-4H-pyrimido[1,2-b]pyridazin-4-one; 9-cyclopropyl-7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl)oxy)piperidin-1-yl)-8-methyl-4H-pyrimido[1,2-b]pyridazin-4-one; 7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)piperidin-1-yl-4-d)-2,8,9-trimethyl-4H-pyrimido[1,2-b]pyridazin-4-one; 7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)piperidin-1-yl-4-d)-8,9-dimethyl-2-(trifluoromethyl)-4H-pyrimido[1,2-b]pyridazin-4-one; 7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)piperidin-1-yl-4-d)-2-isopropyl-8,9-dimethyl-4H-pyrimido[1,2-b]pyridazin-4-one; 9-cyclopropyl-7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)piperidin-1-yl-4-d)-8-methyl-4H-pyrimido[1,2-b]pyridazin-4-one; 7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)piperidin-1-yl-4-d)-2-ethyl-8,9-dimethyl-4H-pyrimido[1,2-b]pyridazin-4-one; 7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)piperidin-1-yl)-2-isopropyl-8,9-dimethyl-4H-pyrimido[1,2-b]pyridazin-4-one; 7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)piperidin-1-yl)-2-ethyl-8,9-dimethyl-4H-pyrimido[1,2-b]pyridazin-4-one; 2-(Cyclopentylmethyl)-7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)piperidin-1-yl-4-d)-8,9-dimethyl-4H-pyrimido[1,2-b]pyridazin-4-one; 7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)piperidin-1-yl-4-d)-8-(methoxymethyl)-9-methyl-4H-pyrimido[1,2-b]pyridazin-4-one; 7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)piperidin-1-yl)-8-(methoxymethyl)-9-methyl-4H-pyrimido[1,2-b]pyridazin-4-one; 7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)piperidin-1-yl-4-d)-9-(methoxymethyl)-8-methyl-4H-pyrimido[1,2-b]pyridazin-4-one; 7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)piperidin-1-yl)-9-(methoxymethyl)-8-methyl-4H-pyrimido[1,2-b]pyridazin-4-one; 8-cyclopropyl-7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)piperidin-1-yl-4-d)-9-methyl-4H-pyrimido[1,2-b]pyridazin-4-one; 8-cyclopropyl-7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)piperidin-1-yl)-9-methyl-4H-pyrimido[1,2-b]pyridazin-4-one; 7-((2R,4R)-4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl)oxy)-2-methylpiperidin-1-yl)-8-methyl-4H-pyrimido[1,2-b]pyridazin-4-one; 7-((2S,4R)-4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl)oxy)-2-methylpiperidin-1-yl)-8-methyl-4H-pyrimido[1,2-b]pyridazin-4-one; 7-((2R,4S)-4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl)oxy)-2-methylpiperidin-1-yl)-8-methyl-4H-pyrimido[1,2-b]pyridazin-4-one; 7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)piperidin-1-yl-4-d)-2-(methoxymethyl)-8-methyl-4H-pyrimido[1,2-b]pyridazin-4-one; 7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)piperidin-1-yl)-2-(methoxymethyl)-8-methyl-4H-pyrimido[1,2-b]pyridazin-4-one; 7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)piperidin-1-yl-4-d)-2-(methoxymethyl)-8,9-dimethyl-4H-pyrimido[1,2-b]pyridazin-4-one; 7-((3S,4R)-4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)-3-fluoropiperidin-1-yl)-8-methyl-4H-pyrimido[1,2-b]pyridazin-4-one; 7-((3R,4S)-4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)-3-fluoropiperidin-1-yl)-8-methyl-4H-pyrimido[1,2-b]pyridazin-4-one; 7-((3R,4S)-4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl)oxy)-3-methylpiperidin-1-yl)-8-methyl-4H-pyrimido[1,2-b]pyridazin-4-one; 7-((3R,4R)-4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl)oxy)-3-methylpiperidin-1-yl)-8-methyl-4H-pyrimido[1,2-b]pyridazin-4-one; 7-((3R,4R)-4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl)oxy)-3-methylpiperidin-1-yl)-8-methyl-4H-pyrimido[1,2-b]pyridazin-4-one; 7-((2R,4R)-4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl)oxy)-2-methylpiperidin-1-yl)-8-methyl-4H-pyrimido[1,2-b]pyridazin-4-one; 7-((3S,4S)-4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)-3-fluoropiperidin-1-yl)-8,9-dimethyl-4H-pyrimido[1,2-b]pyridazin-4-one; 7-((3S,4R)-4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)-3-fluoropiperidin-1-yl)-8,9-dimethyl-4H-pyrimido[1,2-b]pyridazin-4-one; 7-((3R,4S)-4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)-3-fluoropiperidin-1-yl)-8,9-dimethyl-4H-pyrimido[1,2-b]pyridazin-4-one; 7-((3S,4S)-4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)-3-fluoropiperidin-1-yl)-2-(methoxymethyl)-8,9-dimethyl-4H-pyrimido[1,2-b]pyridazin-4-one; 7-((3S,4R)-4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)-3-fluoropiperidin-1-yl)-2-(methoxymethyl)-8,9-dimethyl-4H-pyrimido[1,2-b]pyridazin-4-one; 7-((3R,4S)-4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)-3-fluoropiperidin-1-yl)-2-(methoxymethyl)-8,9-dimethyl-4H-pyrimido[1,2-b]pyridazin-4-one; 7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl)oxy)piperidin-1-yl)-8-methyl-9-(methylamino)-4H-pyrimido[1,2-b]pyridazin-4-one; 7-((3R,4R)-4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)-3-fluoropiperidin-1-yl)-2-(methoxymethyl)-8,9-dimethyl-4H-pyrimido[1,2-b]pyridazin-4-one; 7-((3R,4R)-4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)-3-fluoropiperidin-1-yl)-2-(methoxymethyl)-8-methyl-4H-pyrimido[1,2-b]pyridazin-4-one; 7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl)oxy)piperidin-1-yl)-9-methoxy-8-methyl-4H-pyrimido[1,2-b]pyridazin-4-one; 9-(azetidin-1-yl)-7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl)oxy)piperidin-1-yl)-8-methyl-4H-pyrimido[1,2-b]pyridazin-4-one; 7-((3R,4R)-4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)-3-fluoropiperidin-1-yl)-8,9-dimethyl-4H-pyrimido[1,2-b]pyridazin-4-one; 7-((2R,4S)-4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)-2-methylpiperidin-1-yl)-8-methyl-4H-pyrimido[1,2-b]pyridazin-4-one; 7-((2R,4S)-4-((8-fluoro-2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)-2-methylpiperidin-1-yl)-8-methyl-4H-pyrimido[1,2-b]pyridazin-4-one; 7-((2R,4S)-4-((8-fluoro-2,3-dihydrobenzo[b][1,4]dioxin-6-yl)oxy)-2-methylpiperidin-1-yl)-8-methyl-4H-pyrimido[1,2-b]pyridazin-4-one; 7-((2R,4S)-4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)-2-methylpiperidin-1-yl)-8,9-dimethyl-4H-pyrimido[1,2-b]pyridazin-4-one; 7-((2R,4S)-4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)-2-methylpiperidin-1-yl)-2-(methoxymethyl)-8,9-dimethyl-4H-pyrimido[1,2-b]pyridazin-4-one; 7-((2R,4S)-4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl)oxy)-2-methylpiperidin-1-yl)-8,9-dimethyl-4H-pyrimido[1,2-b]pyridazin-4-one; 7-(4-((8-fluoro-2,3-dihydrobenzo[b][1,4]dioxin-6-yl)oxy)piperidin-1-yl)-8-methyl-4H-pyrimido[1,2-b]pyridazin-4-one; 7-(4-((8-fluoro-2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)piperidin-1-yl)-8-methyl-4H-pyrimido[1,2-b]pyridazin-4-one; 7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)piperidin-1-yl-2,2,6,6-d4)-8-methyl-4H-pyrimido[1,2-b]pyridazin-4-one; or a pharmaceutically acceptable salt thereof.

30. The compound of claim 29 or a pharmaceutically acceptable salt thereof, wherein the compound is 2-(difluoromethyl)-7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)piperidin-1-yl)-8,9-dimethyl-4H-pyrimido[1,2-b]pyridazin-4-one.

31. The compound of claim 29 or a pharmaceutically acceptable salt thereof, wherein the compound is 3-chloro-7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)piperidin-1-yl)-2,8-dimethyl-4H-pyrimido[1,2-b]pyridazin-4-one.

32. The compound of claim 29 or a pharmaceutically acceptable salt thereof, wherein the compound is 7-((3R,4R)-4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)-3-fluoropiperidin-1-yl)-2,8-dimethyl-4H-pyrimido[1,2-b]pyridazin-4-one.

33. The compound of claim 29 or a pharmaceutically acceptable salt thereof, wherein the compound is 7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl)oxy)piperidin-1-yl-4-d)-8,9-dimethyl-4H-pyrimido[1,2-b]pyridazin-4-one.

34. The compound of claim 29 or a pharmaceutically acceptable salt thereof, wherein the compound is 7-(4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)piperidin-1-yl-4-d)-2-(methoxymethyl)-8-methyl-4H-pyrimido[1,2-b]pyridazin-4-one.

35. The compound of claim 29 or a pharmaceutically acceptable salt thereof, wherein the compound is 7-((2R,4S)-4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)-2-methylpiperidin-1-yl)-8-methyl-4H-pyrimido[1,2-b]pyridazin-4-one.

36. The compound of claim 29 or a pharmaceutically acceptable salt thereof, wherein the compound is 7-((2R,4S)-4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl-2,2,3,3-d4)oxy)-2-methylpiperidin-1-yl)-8,9-dimethyl-4H-pyrimido[1,2-b]pyridazin-4-one.

37. A pharmaceutical composition comprising a compound according to any one of claims 1 to 36 or a pharmaceutically acceptable salt thereof, and a pharmaceutically acceptable carrier.

38. Use of a compound or pharmaceutical composition as described in any one of claims 1 to 37, or a pharmaceutically acceptable salt thereof, in the manufacture of a medicament for treating a disease selected from schizophrenia, borderline personality disorder, Tourette's syndrome, bipolar disorder, tardive dyskinesia, Huntington's disease, sleep disorders, pain disorders and cognitive disorders.

39. The use according to claim 38, wherein the disease is Alzheimer's disease.

40. Use of a compound or pharmaceutical composition as described in any one of claims 1 to 37, or a pharmaceutically acceptable salt thereof, in the manufacture of a medicament for treating a disease selected from psychosis, autistic disorder and movement disorders.

41. Use of a compound or pharmaceutical composition as claimed in any one of claims 1 to 37, or a pharmaceutically acceptable salt thereof, in the manufacture of a medicament for treating a disease selected from Tourette's syndrome and akinesia-rigidity syndrome.

42. A compound or pharmaceutical composition as described in any one of claims 1 to 37, or a pharmaceutically acceptable salt thereof, for use in the manufacture of a medicament for the treatment of a disease selected from schizophrenia, conduct disorder, bipolar disorder, anxiety associated with psychosis, mood disorders associated with psychotic disorders, acute mania, obsessive-compulsive disorder and memory disorders.

43. Use of a compound or pharmaceutical composition as described in any one of claims 1 to 37, or a pharmaceutically acceptable salt thereof, in the manufacture of a medicament for treating disruptive behavior disorders.

44. Use of a compound or pharmaceutical composition as described in any one of claims 1 to 37, or a pharmaceutically acceptable salt thereof, in the manufacture of a medicament for treating a disease selected from depression associated with bipolar disorder and mood disorders associated with schizophrenia.

45. Use of a compound or pharmaceutical composition as described in any one of claims 1 to 37, or a pharmaceutically acceptable salt thereof, in the manufacture of a medicament for treating a disease selected from dyskinesia associated with Parkinson's disease, tardive dyskinesia, and drug-induced and neurodegenerative based dyskinesias.

46. ​​Use of a compound or pharmaceutical composition as described in any one of claims 1 to 37, or a pharmaceutically acceptable salt thereof, in the manufacture of a medicament for treating attention deficit hyperactivity disorder.

47. Use of a compound or pharmaceutical composition as described in any one of claims 1 to 37, or a pharmaceutically acceptable salt thereof, in the manufacture of a medicament for treating cognitive impairment.

48. Use of a compound or pharmaceutical composition as described in any one of claims 1 to 37, or a pharmaceutically acceptable salt thereof, in the manufacture of a medicament for treating dementia.

49. Use of a compound or pharmaceutical composition as claimed in any one of claims 1 to 37, or a pharmaceutically acceptable salt thereof, in the manufacture of a medicament for treating mental retardation.

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