Feed additive composition and application thereof

Abstract

The present invention relates to the field of feed additive technology, and in particular to a feed additive composition and application thereof. The present invention provides a feed additive composition, wherein the composition comprises ursolic acid and flavonoid glycosides in a mass ratio of (0.5-5): 1. The present invention finds that ursolic acid and flavonoid glycosides can play a synergistic effect in reducing the mortality rate of livestock and poultry. In the case of equal dietary addition, the effect of ursolic acid and flavonoid glycosides used in combination is significantly higher than that of ursolic acid and flavonoid glycosides added separately. The feed additive composition provided by the present invention can effectively reduce the mortality rate of livestock and poultry, improve the growth performance of livestock and poultry, promote their weight gain, increase average daily feed intake and average daily weight gain, reduce feed-to-weight ratio to a certain extent, and is of great significance for the development of feed additives for replacing antibiotics.

Description

Technical Field

[0001] The present invention relates to the technical field of feed additives, in particular to a feed additive composition and application thereof. Background Art

[0002] Animal husbandry plays a vital role in improving residents' dietary structure, providing animal protein, and promoting economic development. However, due to the widespread increase in intensive animal husbandry practices in recent years, many environmental factors can affect the growth and development of livestock and poultry, leading to high mortality rates and significant economic losses for livestock farmers.

[0003] Antibiotics have long been added to animal feed for their disease prevention and growth-promoting properties. However, due to concerns about drug residues, drug resistance, and potential impacts on human health, their use is increasingly being regulated and restricted in various countries and regions. Reducing diarrhea and lowering mortality rates in livestock and poultry without the use of antibiotics has become a major challenge facing the livestock industry. Therefore, there is an urgent need to develop green, effective, non-toxic, and residue-free feed additives that can promote growth and maintain animal health while effectively reducing mortality rates. Summary of the Invention

[0004] The invention provides a feed additive composition and application thereof.

[0005] Specifically, the present invention provides the following technical solutions:

[0006] The invention provides a feed additive composition, which comprises ursolic acid and flavonoid glycoside compounds in a mass ratio of (0.5-5):1.

[0007] In the process of screening feed additive components and their combinations, the present invention unexpectedly discovered that ursolic acid and flavonoid glycoside compounds can play a synergistic role in reducing the mortality rate of livestock and poultry and improving the growth performance of livestock and poultry within the above-mentioned ratio range. Under the condition of the same daily dietary addition amount, the effect of ursolic acid and flavonoid glycoside compounds in combination is significantly higher than that of ursolic acid and flavonoid glycoside compounds added alone.

[0008] Preferably, the flavonoid glycoside compound is luteolin-7-O-β-D-glucuronide and / or isorhamnetin-3-O-glucoside.

[0009] In some embodiments of the present invention, the flavonoid glycoside compound is luteolin-7-O-β-D-glucuronide or isorhamnetin-3-O-glucoside.

[0010] When the above-mentioned flavonoid glycosides are used in combination with ursolic acid, the mass ratio of ursolic acid to flavonoid glycosides in the composition is preferably (0.5-3):1.

[0011] The present invention found that within the above ratio range, ursolic acid can play a synergistic role with luteolin-glucuronic acid ester, isorhamnetin-glucose, and flavonoids-hexose in reducing the mortality rate of livestock and poultry and promoting the growth performance of livestock and poultry.

[0012] In one embodiment of the present invention, the flavonoid glycoside compound is luteolin-7-O-β-D-glucuronide, and in the composition, the mass ratio of ursolic acid to luteolin-7-O-β-D-glucuronide is (0.75-3):1.

[0013] In one embodiment of the present invention, the flavonoid glycoside compound is isorhamnetin-3-O-glucoside, and in the composition, the mass ratio of ursolic acid to isorhamnetin-3-O-glucoside is (0.5-2):1, preferably (0.5-1.5):1.

[0014] The present invention provides a method for preparing the feed additive composition described above, which comprises: mixing ursolic acid with a flavonoid glycoside compound.

[0015] The present invention also provides use of the above feed additive composition in feed additive or feed preparation.

[0016] The above application in the preparation of feed additives includes: mixing the feed additive composition with other feed additive components to obtain the feed additive, or mixing the feed additive composition with other feed additive components and / or auxiliary materials to obtain the feed additive.

[0017] The above application in feed preparation includes: mixing the feed additive composition with a basic diet feed to obtain feed, or mixing the feed additive composition with a basic diet feed and / or supplementary materials to obtain feed.

[0018] The above-mentioned auxiliary materials can be any auxiliary materials allowed in the feed field.

[0019] The present invention provides a feed comprising the above feed additive composition.

[0020] Preferably, the mass percentage of the feed additive composition in the feed is 0.01-0.1%.

[0021] The present invention provides the above-mentioned feed additive composition or application of the feed in reducing the mortality rate of livestock and poultry.

[0022] The present invention also provides the above feed additive composition or use of the feed in improving the growth performance of livestock and poultry.

[0023] Preferably, the improved growth performance is manifested as increased body weight, increased average daily gain, increased average daily feed intake and / or reduced feed-to-gain ratio.

[0024] Preferably, the application comprises: administering the feed additive composition or the feed to livestock and poultry, wherein the administration amount of the feed additive composition is 250-750 mg per kilogram of basic diet.

[0025] In the present invention, the livestock and poultry include but are not limited to chickens, ducks, geese, pigs, cattle, sheep, horses and other poultry and livestock.

[0026] Preferably, the livestock and poultry are chickens, more preferably broilers.

[0027] The present invention has the following beneficial effects: The present invention discovered that ursolic acid and flavonoid glycoside compounds can play a synergistic role in reducing the mortality rate of livestock and poultry. Under the condition of equal dietary addition, the effect of ursolic acid and flavonoid glycoside compounds in combination is significantly higher than that of ursolic acid or flavonoid glycoside compounds added alone. The feed additive composition provided by the present invention can effectively reduce the mortality rate of livestock and poultry, improve the growth performance of livestock and poultry, promote their weight gain, increase their average daily feed intake and average daily weight gain, and reduce the feed-to-weight ratio to a certain extent. It is of great significance for the development of feed additives that can replace antibiotics. DETAILED DESCRIPTION

[0028] To make the objectives, technical solutions, and advantages of the present invention more clear, the technical solutions of the present invention are described clearly and completely below. Obviously, the embodiments described are only some of the embodiments of the present invention, not all of them. All other embodiments derived by persons of ordinary skill in the art based on the embodiments of the present invention without inventive effort are within the scope of protection of the present invention.

[0029] Example 1

[0030] This embodiment provides a feed additive composition, which is composed of the following components in parts by weight: 30 parts of ursolic acid and 40 parts of luteolin-7-O-β-D-glucuronide.

[0031] Example 2

[0032] This embodiment provides a feed additive composition, which is composed of the following components in parts by weight: 40 parts of ursolic acid and 35 parts of isorhamnetin-3-O-glucoside.

[0033] Example 3

[0034] This embodiment provides a feed additive composition, which is composed of the following components in parts by weight: 60 parts of ursolic acid and 20 parts of luteolin-7-O-β-D-glucuronide.

[0035] Example 4

[0036] This embodiment provides a feed additive composition, which is composed of the following components in parts by weight: 40 parts of ursolic acid and 80 parts of isorhamnetin-3-O-glucoside.

[0037] Comparative Example 1

[0038] This comparative example provides a feed additive composition, which is composed of the following components in parts by weight: 60 parts of ursolic acid and 20 parts of kaempferol-3-O-sophoroside.

[0039] Comparative Example 2

[0040] This comparative example provides a feed additive consisting only of luteolin-7-O-β-D-glucuronide.

[0041] Comparative Example 3

[0042] This comparative example provides a feed additive consisting only of ursolic acid.

[0043] Comparative Example 4

[0044] This comparative example provides a feed additive consisting only of isorhamnetin-3-O-glucoside.

[0045] Experimental example

[0046] The feed additive compositions of the examples and comparative examples were used to raise broiler chickens to detect their effects on the mortality rate, growth performance, etc. of the broiler chickens.

[0047] 1. Experimental Design

[0048] 1,296 healthy, one-day-old AA male broiler chickens of similar weight were randomly divided into nine groups, with six replicates per group and 24 chickens per replicate. The control group was fed a corn-soybean meal basal diet, a powdered feed formulated according to the NRC (1994) guidelines. Its composition and nutritional levels are shown in Table 1. The experimental groups were fed a basal diet supplemented with the feed additive compositions of various Examples and Comparative Examples. The feed additive composition of Example 1 was added at a rate of 250 mg / kg of basal diet, the feed additive composition of Example 2 was added at a rate of 250 mg / kg of basal diet, the feed additive composition of Example 3 was added at a rate of 250 mg / kg of basal diet, the feed additive composition of Example 4 was added at a rate of 250 mg / kg of basal diet, and the feed additive compositions of Comparative Examples 1-4 were added at a rate of 250 mg / kg of basal diet. The experimental period was 42 days.

[0049] Table 1 Basic diet composition and nutrient levels (air-dried basis)

[0050]

[0051]

[0052] Note: 1) Premix provides the following per kg of feed: Vitamin A 12,500 IU, Vitamin D3 2,600 IU, Vitamin E 50 IU, Vitamin K 34 mg, Riboflavin 5.00 mg, D-calcium pantothenate 24 mg, Niacin 55 mg, Vitamin B12 0.03 mg, Manganese (MnSO4·H2O) 55 mg, Iron (FeSO4·H2O) 75 mg, Zinc (ZnSO4·7H2O) 36 mg, Copper (CuSO4·5H2O) 8 mg, and Selenium (Na2SeO3) 0.15 mg. 2) Nutrient levels are calculated values.

[0053] 2. Feeding and management

[0054] The experimental broilers were housed in three-tiered cages. The broiler houses and equipment were thoroughly rinsed and disinfected before the experiment. The broiler houses were temperature-controlled with electric intelligent heaters. Continuous lighting, natural ventilation, and longitudinal negative pressure ventilation were used. During the experimental period, lighting, relative humidity, and temperature were controlled according to AA broiler husbandry and management requirements. The broiler houses were cleaned regularly, and immunizations were performed according to normal procedures. Throughout the experimental period, the birds had free access to food and water. The temperature and humidity in the broiler houses were recorded daily at 08:00, 12:00, and 18:00. During the experimental period, the feed intake, water intake, and health status of the test birds were observed and recorded daily. Mortalities and feed consumption were also recorded.

[0055] 3. Measurement indicators and methods

[0056] 3.1 Death rate

[0057] The number of broiler chickens that died at 1 to 21 days of age, 22 to 42 days of age, and 1 to 42 days of age was counted, and the mortality rate (%) was calculated.

[0058] 3.2 Growth performance indicators

[0059] Broilers were weighed in replicates at 1, 21, and 42 days of age, and their feed intake was recorded from 1 to 21 days, 22 to 42 days, and 1 to 42 days of age. The average daily feed intake (ADFI), average daily gain (ADG), and feed-to-gain ratio (F / G) were also calculated.

[0060] The calculation method of the above indicators is as follows:

[0061] ADG = (final weight - initial weight) / number of days;

[0062] ADFI = total feed intake / (number of experimental days × total number of experimental chickens);

[0063] F / G = average daily feed intake / average daily gain.

[0064] 4. Data statistical analysis

[0065] All experimental data were analyzed by one-way analysis of variance using SPSS 20 software.

[0066] 5. Test results

[0067] 5.1 Mortality rate

[0068] The results are shown in Table 2. As can be seen from Table 2, compared with the control group and each comparative example, the feed additive composition of each embodiment can reduce the mortality rate of broiler chickens in the early growth stage, late growth stage, and full growth stage. Among them, the mortality rate of the feed additive composition of each embodiment during the full growth stage was significantly lower than that of the control group and each comparative example. This shows that, under the condition of equal dietary addition, the effect of the feed additive composition of each embodiment on reducing the mortality rate is significantly better than the effect of each component acting alone (Comparative Examples 2-3). In the feed additive composition of each embodiment, the components have a synergistic effect in reducing the mortality rate of livestock and poultry.

[0069] Table 2 Effects of adding different compositions to the diet on the mortality rate of broiler chickens

[0070]

[0071] 5.2 Growth performance indicators

[0072] The results are shown in Table 3. As can be seen from Table 3, adding the composition of each embodiment to the diet significantly improved the growth performance of broiler chickens during the late growth period (22-42 days) and the entire growth period (1-42 days). Compared with the control group, adding the composition of each embodiment significantly increased the body weight of broiler chickens at 42 days (P < 0.05); adding the compositions of Examples 1, 2, 3, and 4 all significantly increased the average daily weight gain of broiler chickens from 22 to 42 days (P < 0.05). However, when the compositions of Comparative Examples 1, 2, 3, and 4 were added, the body weight of broiler chickens at 42 days was not significantly different from that of the control group, and the body weight of broiler chickens added with the compositions of each embodiment at 42 days was significantly higher than that of broiler chickens added with the compositions of Comparative Examples 1 and 2. At the same time, adding the compositions of each embodiment significantly reduced the feed-to-weight ratio from 22 to 42 days and from 1 to 42 days.

[0073] Table 3 Effects of adding different compositions to the diet on the growth performance of broilers

[0074]

[0075] In summary, the feed additive composition provided by the present invention can significantly reduce the mortality rate of broilers in the early growth period (1-21d) and the entire growth period (1-42d), and significantly reduce the mortality rate of broilers in the late growth period; at the same time, it can also effectively improve the growth performance of livestock and poultry, and can significantly increase the average daily weight gain of broilers in the late growth period (22-42d) and the entire growth period (1-42d), and significantly reduce the feed-to-weight ratio in the late growth period (22-42d) and the entire growth period (1-42d).

[0076] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or make equivalent replacements for some of the technical features therein. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions of the various embodiments of the present invention.

Claims

1. A feed additive composition, characterized in that The composition comprises ursolic acid and flavonoid glycoside compounds in a mass ratio of (0.5-5):1; The flavonoid glycoside compound is luteolin-7-O-β-D-glucuronide and / or isorhamnetin-3-O-glucoside.

2. The feed additive composition according to claim 1, characterized in that In the composition, the mass ratio of ursolic acid to flavonoid glycosides is (0.5-3):

1.

3. The feed additive composition according to claim 1 or 2, characterized in that The flavonoid glycoside compound is luteolin-7-O-β-D-glucuronide, and in the composition, the mass ratio of ursolic acid to luteolin-7-O-β-D-glucuronide is (0.75-3):1; Alternatively, the flavonoid glycoside compound is isorhamnetin-3-O-glucoside, and in the composition, the mass ratio of ursolic acid to isorhamnetin-3-O-glucoside is (0.5-2):

1.

4. Use of the feed additive composition according to any one of claims 1 to 3 in feed additive or feed preparation.

5. A feed, characterized in that The feed comprises the feed additive composition according to any one of claims 1 to 3.

6. The feed according to claim 5, characterized in that In the feed, the mass percentage of the feed additive composition is 0.01-0.1%.

7. Use of the feed additive composition according to any one of claims 1 to 3 or the feed according to claim 5 or 6 in reducing the mortality rate of livestock and poultry.

8. Use of the feed additive composition according to any one of claims 1 to 3 or the feed according to claim 5 or 6 in improving the growth performance of livestock and poultry.

9. The use according to claim 8, characterized in that The improvement of growth performance is manifested in the application of increasing body weight, increasing average daily weight gain, increasing average daily feed intake and / or reducing feed-to-weight ratio.

10. The use according to any one of claims 7 to 9, characterized in that: The application comprises: administering the feed additive composition or the feed to livestock and poultry, wherein the administration amount of the feed additive composition is 250-750 mg per kilogram of basic diet.

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