A plant nanogene delivery formulation, a method for plant nanogene delivery and functional protein expression, and its applications.
By modifying mesoporous silica nanoparticles and using ultrasound-assisted technology, the problem of low transfection efficiency in plant cells was solved, enabling efficient and safe expression and extraction of functional proteins, thus overcoming the biosafety risks of traditional methods.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- ZHEJIANG UNIV
- Filing Date
- 2023-02-22
- Publication Date
- 2026-05-26
AI Technical Summary
Existing plant cell transfection methods are inefficient, pose biosafety risks, and are difficult to express functional proteins efficiently. Traditional transformation vectors, such as Agrobacterium, are inefficient and physical strategies cause significant cell damage. The application of nanotechnology in plant gene delivery has not yet involved protein expression.
Using mesoporous silica nanoparticles (MSN) as carriers, plant nanogene delivery formulations were prepared through cation modification and cell-penetrating peptide modification, combined with ultrasound-assisted technology. These formulations promote nucleic acid entry into plant cells and insert secretory peptide sequences into the nucleic acids to enhance protein expression.
It improves gene delivery efficiency, reduces damage to plant cells, enhances the secretion and extraction and purification efficiency of functional proteins, and achieves efficient and safe expression of functional proteins.
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