A bactericidal composition containing benzovindiflupyr and procymidone and its application

Abstract

The present invention provides a bactericidal composition containing benzovindiflupyr and procymidone and its application. The characteristics of the application of the bactericidal composition are as follows: the mass ratio of benzovindiflupyr to procymidone in terms of 100% active ingredient is 1:1 - 1:6, preferably 1:3. The bactericidal composition and its application of the present invention have a significant synergistic effect on Sclerotinia sclerotiorum of rape, can reduce the usage amount of pesticides, reduce costs, reduce the harm to the environment, and are beneficial to delaying the generation of pathogen drug resistance.

Description

Technical Field

[0001] The present invention belongs to the field of agrochemistry, and particularly relates to a bactericidal composition containing benzovindiflupyr and procymidone and its application. Background Art

[0002] Sclerotinia sclerotiorum of rapeseed is a worldwide disease, occurring throughout the country, and is most serious in the Yangtze River Basin, with an incidence rate of 10 - 80% and a yield loss of 5 - 30%. Sclerotinia sclerotiorum of rapeseed is mainly caused by the infection of fungi in the Ascomycotina. At present, the pesticides used for field control mainly rely on single active substances, and the application level is relatively low. Due to the annual use, the resistance of the disease to a certain active substance develops relatively fast, and the dosage of the pesticide is increased year by year, resulting in certain environmental pollution and food safety problems. The combined use of two active substances with different action mechanisms is an effective means to delay the occurrence of resistance and reduce the use of pesticides. Through Sun Yunpei's co-toxicity coefficient method, it is judged whether the combined use of two active substances is synergistic, additive, or antagonistic to the target pathogen. If the combined use of two active substances has a significant synergistic effect, then the combined use of the two active substances can reduce the use of pesticides and delay the generation of disease resistance, which is an important means for comprehensive disease control.

[0003] There is no relevant report on the combined use of benzovindiflupyr and procymidone to control Sclerotinia sclerotiorum of rapeseed in existing domestic and foreign technologies.

[0004] Benzovindiflupyr is a pyrazole amide fungicide developed by Syngenta. It is a succinate dehydrogenase inhibitor (SDHI), belonging to the pathogen respiration inhibitor. It inhibits the function of mitochondria by interfering with the tricarboxylic acid cycle on respiratory electron transport chain complex II, preventing it from generating energy, inhibiting the growth of pathogens, and ultimately causing their death. Benzovindiflupyr has no cross-interaction with methoxyacrylate and triazole fungicides. Benzovindiflupyr is broad-spectrum, highly effective, and has a long-lasting effect. It has systemic conductivity and can tightly bind to the plant cuticle and slowly penetrate into plant tissues. It has good effects on leaf blight, powdery mildew, rust, take-all, basal rot on wheat, peanut black spot, maize southern leaf blight, and gray mold. Since benzovindiflupyr belongs to the SDHI class of fungicides with a single action site and a relatively high level of resistance development, the International Fungicide Resistance Action Committee (FRAC) recommends that this type of fungicide be combined or rotated with fungicides with different action mechanisms to delay the development of resistance.

[0005] Procymidone was developed by Sumitomo Chemical Industry Co., Ltd. of Japan. It mainly inhibits the synthesis of triglycerides in the mycelium, mainly acts on the cell membrane, hinders the normal cell wall synthesis at the tip of the hypha, and inhibits the development of the hypha. Due to years of use, its effect in controlling Sclerotinia sclerotiorum of rapeseed is not ideal, and the rapeseed yield is reduced due to the poor control effect of Sclerotinia sclerotiorum. Summary of the Invention

[0006] To solve the problems existing in the prior art, the present invention provides the following technical solutions.

[0007] A bactericidal composition containing benzovindiflupyr and procymidone is used for controlling Sclerotinia sclerotiorum of rapeseed, which is characterized in that: the active ingredients comprise benzovindiflupyr and procymidone, and the mass ratio of the benzovindiflupyr and procymidone after converting to 100% is 1:1 - 1:6.

[0008] For the bactericidal composition and its application of the present invention, it is characterized in that: the mass ratio of the benzovindiflupyr and procymidone after converting to 100% is preferably 1:3.

[0009] For the bactericidal composition and its application of the present invention, it is characterized in that: the processable dosage forms of the bactericidal composition are wettable powders, water dispersible granules, suspension concentrates, etc.

[0010] For the bactericidal composition and its application of the present invention, it is characterized in that: the bactericidal composition is used for controlling crop diseases, especially Sclerotinia sclerotiorum of rapeseed.

[0011] Compared with the prior art, the present invention has the following excellent effects.

[0012] The bactericidal composition and its application of the present invention are composed of two active ingredients with different action mechanisms. After mixing, they have a significant synergistic effect on Sclerotinia sclerotiorum of rapeseed, improving the control effect, reducing the usage amount of pesticides, lowering the cost, reducing the harm to the environment, and being beneficial to delaying the generation of pathogen drug resistance. It has good safety for crops and meets the safety requirements of pesticide formulations. Specific embodiments

[0013] The following combines examples to elaborate in detail on the excellent effects of the present invention, but it should be understood that the protection scope of the present invention is not limited by specific examples.

[0014] Example 1 Indoor toxicity determination of benzovindiflupyr, procymidone and their mixed use against Sclerotinia sclerotiorum of rapeseed

[0015] Control object: Sclerotinia sclerotiorum of rapeseed was obtained by isolation and purification from the field by this unit.

[0016] Source of medicament: Provided by the drug preparation room of this company.

[0017] Test methods and steps: All treatments were pre-tested in advance to determine the appropriate experimental concentrations for each treatment. The correct selection of concentrations is the key to ensuring the success of the experiment.

[0018] After converting the benzovindiflupyr and procymidone technical materials to 100%, dispersants, wetting agents, thickeners and water were added to prepare 10% benzovindiflupyr suspension concentrate and 50% procymidone suspension concentrate respectively, and quantitative analysis method was used for content calibration.

[0019] All the following steps are carried out under sterile conditions. The utensils, pipettes, gun tips, punchers, sterile water, etc. used are sterilized by wet heat sterilization at 121°C for 30 minutes in a pressure cooker. The surface of the agent is wiped and disinfected with 75% disinfectant alcohol multiple times from the outer chamber to the inner chamber. 10% benzovinyl flubacillin suspension, 50% procymidone suspension, and a mixture of benzovinyl flubacillin and procymidone are added to sterile water to prepare the corresponding gradient mother solution for standby use. Use a pipette to measure and add to the sterilized PDA culture medium, shake well, and pour into a sterilized 9cm culture dish. Repeat each treatment 3 times to prepare the corresponding drug-containing plate. Set up a blank plate for control without adding drugs. After the plate solidifies, use a puncher with an inner diameter of 7mm to cut the bacterial cake from the edge of the colony of the pre-cultivated bacteria-containing (rapeseed sclerotinia) culture medium. Place the bacterial cake in the center of the drug-containing culture medium, with the mycelium side facing downward (the mycelium side is in contact with the culture medium), and place one bacterial cake in each culture dish. Place the inoculated culture dish in a constant temperature biochemical incubator at 26 degrees Celsius for culture. When the control culture medium just fills the culture dish, use the cross method to measure the colony diameter vertically with a vernier caliper, calculate the average value, and calculate the mycelium growth inhibition rate at each treatment concentration of each agent.

[0020]

[0021] Use DPS data processing system to analyze and calculate the regression curve and EC 50 The synergistic effect of drug mixing was evaluated according to the co-toxicity coefficient method (CTC), that is, CTC ≤ 80 was antagonistic, 80 < CTC < 120 was additive, and CTC ≥ 120 was synergistic.

[0022]

[0023] Theoretical toxicity index (TTI) of mixture = toxicity index of agent A × percentage of agent A in the mixture (%) + toxicity index of agent B × percentage of agent B in the mixture (%)

[0024]

[0025] Test results: Table 1 Indoor toxicity test of benzovinflumizole and procymidone and their mixture against Sclerotinia sclerotiorum of rapeseed

[0026]

[0027] As can be seen from Table 1, in the bactericidal composition of the present invention, the mass ratio of benzovindiflupyr and procymidone in terms of 100% purity is in the range of 10:1 - 1:10, and their co-toxicity coefficient CTC is greater than 120, showing a synergistic effect on Sclerotinia sclerotiorum of rapeseed. In particular, when the mass ratio of benzovindiflupyr and procymidone in terms of 100% purity is in the range of 1:1 - 1:6, the CTC value is between 172.36 - 262.32, showing a significant synergistic effect on Sclerotinia sclerotiorum of rapeseed. Moreover, when the mass ratio of benzovindiflupyr and procymidone in terms of 100% purity is 1:3, the co-toxicity coefficient is 262.32, which is the highest among the tested ratios and shows the most significant synergistic effect. From the above joint toxicity experiments, it is proved that the mixed use of benzovindiflupyr and procymidone in the present invention has a synergistic effect, which is scientific and practical and meets the industry requirements of the country for pesticide formulation processing.

[0028] Example 2 10% Benzovindiflupyr·Procymidone Wettable Powder (1:1)

[0029] Benzovindiflupyr 5%, Procymidone 5%, Sodium dodecyl sulfate 1%, Morwet D - 425 (alkylnaphthalenesulfonate formaldehyde condensate) 5%, White carbon black 5%, Kaolin remainder.

[0030] Example 3 40% Benzovindiflupyr·Procymidone Suspension Concentrate (1:3)

[0031] Benzovindiflupyr 10%, Procymidone 30%, Genapol X080 1% (isotridecyl alcohol polyoxyethylene ether), Dispersogen LFS (polyarylalkylphenol polyethylene glycol phosphate ester) 5%, Agrilan 788 (hydrophobic group modified carboxylic acid copolymer) 2%, Ethylene glycol 5%, Sag1522 (organosilicon defoamer) 0.2%, Magnesium aluminum silicate 1%, Xanthan gum 0.1%, Kathon 0.1%, Water remainder.

[0032] Example 4 30% Benzovindiflupyr·Procymidone Water Dispersible Granules (1:2)

[0033] Benzovindiflupyr 10%, Procymidone 20%, Morwet EFW (alkylnaphthalenesulfonate condensate) 3%, Morwet D - 425 (alkylnaphthalenesulfonate formaldehyde condensate) 5%, Tersperse 2700 (polycarboxylate dispersant) 2%, Attapulgite remainder.

[0034] Example 5 Field Effect Verification

[0035] The test set two dosages of 40% Benzovindiflupyr·Procymidone Suspension Concentrate (1:3) at 400 mL·hm -2、 、600 mL·hm -2 ; 50% Procymidone Wettable Powder at 1200 mL·hm -2、40% fluazinam·isoprodolil suspension 750mL·hm -2 、200g / L fluopicolide suspension 800mL·hm -2 、100g / L benzovintril suspension 1200mL·hm -2 There were 7 treatments in total, including one dose each and a water control. The same dose of each agent was applied once during the flowering and full flowering periods of rapeseed. An electric sprayer was used to spray evenly from top to bottom, with a water consumption of 750L·hm -2 During the rapeseed maturity period, the disease classification standards of plant protection were used for investigation and statistics.

[0036] The statistical data are shown in Table 2.

[0037] Table 2 Comprehensive control effect of rapeseed sclerotinia after application

[0038]

[0039] The experimental results show that the safety of each treatment agent to rapeseed crops is good after application, and no adverse symptoms such as yellowing leaves, deformed leaves, and deformed fruits were observed due to the application of the agent. 40% benzovintriazole·procymidone suspension (1:3) 400mL·hm -2 、600mL·hm -2 The control effects on rapeseed sclerotinia were 96.43% and 98.21% respectively, which were significantly higher than the control sample 50% procymidone WP 1200mL·hm -2 、40% fluazinam·isoprodolil suspension 750mL·hm -2 、200g / L fluopicolide suspension 800mL·hm -2 、100g / benzovintril suspension 1200mL·hm -2 Preventive effect.

[0040] Any modification, equivalent replacement and alteration made within the spirit and principle of the present invention shall be included in the protection scope of the present invention. The preparation and processing technology of the fungicidal composition of the present invention are all prior art and are well known to professionals in the field. In the prevention and control of diseased crops, the present invention is not limited to rapeseed sclerotinia, and has a good prevention and control effect on ascomycetes.

Claims

1. Application of a bactericidal composition containing benzovindiflupyr and procymidone in controlling Sclerotinia sclerotiorum of rape, characterized in that, the active ingredients are benzovindiflupyr and procymidone, and the mass ratio of benzovindiflupyr and procymidone after discounting to 100% is 1:1 - 1:

6.

2. Application of the bactericidal composition containing benzovindiflupyr and procymidone in controlling Sclerotinia sclerotiorum of rape according to claim 1, characterized in that, the mass ratio of benzovindiflupyr and procymidone after discounting to 100% is 1:

3.

3. Application of the bactericidal composition containing benzovindiflupyr and procymidone in controlling Sclerotinia sclerotiorum of rape according to claim 1 or 2, characterized in that, the bactericidal composition can be prepared into pesticide processing dosage forms of wettable powder, water dispersible granule or suspension concentrate.

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