Apparatus and method for detecting motor learning behavior in mice

Abstract

The application discloses a device and method for detecting mouse motion learning behavior, and belongs to the field of animal behavior detection. The device comprises a mouse motion runway, a dark box at the terminal of the runway, and a camera for recording the motion process of the mouse. The runway is provided with a plurality of replaceable metal rods in the transverse direction. The metal rods are composed of any one or both of white metal rods and black metal rods. The runway composed of the black metal rods and the white metal rods is provided with any one of the metal rods for electrification, and the electrified metal rods are discontinuously arranged. The mouse is shocked by stepping on the electrified metal rods. The method of the application replaces the metal rods to form different runways, trains the mouse to reach the dark box through the different runways, and obtains the motion learning behavior ability of the mouse at different stages. The process of motion learning is simulated through programmed training. The role of the brain regions in the process of motion learning behavior is determined by regulating the regions of the substantia nigra or the striatum corresponding to the motion learning.

Description

Technical Field

[0001] This invention belongs to the field of animal behavior detection, and more specifically, relates to an apparatus and method for detecting the learned motor behavior of mice. Background Technology

[0002] The striatum, the largest nucleus in the basal ganglia, is primarily involved in motor acquisition processes, including learning and memory, motor control, and motor programming. Successful motor acquisition requires transitions between four distinct stages. The first stage is motivation-driven action. The second stage involves insightful behavior, where the subject associates the motor action with a goal, compares appropriate and inappropriate ways to achieve that goal, and begins to repeat the action. The third stage involves the adjustment of motor activity to optimize the goal outcome. In the fourth stage, goal-oriented behavior becomes a skill or habit.

[0003] The striatum primarily receives dopaminergic nerve input from the substantia nigra pars compacta (SNMP). Different parts of the striatum contribute differently to different stages of learned behavior. In the first stage of motor learning, dopaminergic signals in the SNMP support the initial motivation to perform motor actions. Nerve fibers from the SNMP project to the dorsal part of the striatum, facilitating the initiation of motor actions. The second and third stages are mainly involved by the dorsomedial striatum, manifested in the animal's observation of the outcome of a specific motor action and the acquisition of behavior. In this optimization stage, if the goal-oriented behavior is accidentally altered, the animal will easily learn to change its motor strategy. The fourth stage mainly involves the dorsolateral striatum, promoting the consolidation of learned behavior. If the goal-oriented behavior is accidentally altered in this stage, changing the learned behavior becomes more difficult.

[0004] The classic device for assessing learning ability in mice is the Skinner box. However, this device focuses on the learning ability of mice and is mainly used to detect the relationship between damage to learning-related brain regions such as the hippocampus and the animal's learning ability. Since the striatum is involved in the motor learning process, including motor skills, goal-oriented behavior, habit formation, and skill consolidation, the Skinner box cannot effectively detect the relationship between these learning processes and specific subregions of the striatum. Summary of the Invention

[0005] In view of the shortcomings of the prior art, the present invention aims to provide a device for detecting the learned motor behavior of mice; another objective of the present invention is to provide a method for detecting the learned motor behavior of mice.

[0006] To achieve the above objectives, the present invention adopts the following technical solution: a device for detecting mouse movement acquisition behavior, the device comprising a track for mouse movement, a dark box at the end of the track, and a camera for recording the mouse movement process, wherein the track is arranged laterally with multiple replaceable metal rods, the metal rods being composed of any one or two of white metal rods and black metal rods; the track composed of black metal rods and white metal rods is configured to energize any one of the metal rods and the energized metal rods are not arranged continuously, and the mouse is shocked when it steps on the energized metal rod.

[0007] Furthermore, the two sides of the track are made of black plexiglass panels, the track is 200cm long and 3.5cm wide, and the interval between two adjacent metal poles is 1cm.

[0008] Furthermore, the track also includes multiple indicator lights connected to multiple energized metal rods. When a mouse steps on an energized metal rod, the indicator light connected to the energized metal rod flashes.

[0009] A method for detecting motor learning behavior in mice, using the aforementioned device, involves training mice to navigate different tracks to a dark box by replacing metal rods, thereby acquiring the mice's motor learning abilities at different stages. The method includes the following steps:

[0010] (1) Set up a first track using either black or white metal rods, and train the mice on the first track for 2 to 5 days.

[0011] (2) Set the ratio of black metal rods to white metal rods to 1:1. Electricize either black or white metal rods to form a second track. Mice trained on the first track are trained on the second track for 2 to 5 days.

[0012] (3) Increase the number of unpowered metal rods in the second channel and decrease the number of powered metal rods in the second channel. The ratio of unpowered metal rods to powered metal rods is n:1 to form the third track, where n≥1.5. Mice trained in the second track are trained in the third track for 2-5 days or 10-20 days respectively.

[0013] (4) Set the non-energized metal rods in the third track to be energized, and the energized metal rods to be non-energized. The ratio of the number of energized metal rods to the number of non-energized metal rods is 1:m to form the fourth track, where m≥1.5. Mice that have been trained in the third track for 2-5 days or 10-20 days are trained in the fourth track for 2-5 days.

[0014] Furthermore, step (1) also includes testing the time it takes for the mouse trained on the first track to pass through the first track on its own, in order to obtain the mouse's motor ability.

[0015] Furthermore, step (2) also includes testing the error rate of mice that have been trained on the second track stepping on the electrified metal rods when they pass through the second track on their own, in order to obtain the learning ability of mice in goal-oriented behavior.

[0016] Furthermore, step (3) also includes testing the error rate of mice stepping on the electrified metal rods when they pass through the third track after training in the third track, and obtaining the optimization ability of the learned behavior after 2 to 5 days of training, and the consolidation ability of the learned behavior after 10 to 20 days of training.

[0017] Furthermore, step (4) also includes testing the error rate of mice stepping on the electrified metal rods when they pass through the fourth track after training in the fourth track, and obtaining the ability of mice to change their learned behavior after the goal-oriented behavior is changed after 2 to 5 days of training, as well as the ability of mice to change their learned behavior after the goal-oriented behavior is changed after 2 to 5 days of training.

[0018] Furthermore, in steps (1) to (4), the training is conducted 5 times per day.

[0019] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0020] (1) The device of the present invention provides a track for mice to move on. Multiple replaceable metal rods are arranged horizontally on the track. Different tracks are formed by adjusting the ratio of black metal rods to white metal rods in the track and energizing one of the black metal rods or white metal rods. The mice are trained to reach the terminal box through different tracks, and the motor learning behavior ability of mice at different stages is obtained. The relationship between different areas of the striatum and motor learning behavior can be detected.

[0021] (2) Compared with Skinner box, the device of the present invention has the advantages of easy assembly, low cost and easy operation; different tracks are formed by different arrangements of white metal rods and black metal rods for different research purposes, and each track is easy to assemble and disassemble; the present invention tests the error rate of mice stepping on the energized metal rod by setting the indicator light connected to the energized metal rod to flash when the mouse steps on the energized metal rod, and only the obtained image information needs to be statistically analyzed at the end, which is convenient to operate and low in cost;

[0022] (3) The detection method of the present invention utilizes the characteristic that mice can recognize black and white objects, simulates the process of motor learning through programmed training, and clarifies the role of these brain regions in the process of motor learning by regulating the substantia nigra or striatum regions corresponding to motor learning. Attached Figure Description

[0023] Figure 1 This is a schematic diagram of the structure of the device of the present invention;

[0024] Figure 2 This is a schematic top view of the structure of the third runway in Example 2;

[0025] Figure 3 This is a graph showing the time taken for the two groups of mice to traverse the first track independently in Example 1;

[0026] Figure 4 This is a graph showing the error rate of the two groups of mice navigating the second track independently in Example 2.

[0027] Figure 5 This is a graph showing the error rates of the two groups of mice navigating the third and fourth tracks independently in Example 2.

[0028] Figure 6 This is a graph showing the error rates of the two groups of mice navigating the third and fourth tracks independently in Example 3.

[0029] Figure 7 This is a graph showing the error rates of the two groups of mice navigating the third and fourth tracks independently in Example 4.

[0030] Figure 8 This is a graph showing the error rates of the two groups of mice navigating the third and fourth tracks independently in Example 5.

[0031] Among them, 1 is the runway, 2 is the dark box, 3 is the camera, and 4 is the indicator light. Detailed Implementation

[0032] The present invention will be further described below with reference to the accompanying drawings and embodiments.

[0033] This invention provides a device for detecting motor learning behavior in mice, such as... Figure 1 and Figure 2 As shown, the device includes a track 1 for mouse movement, a dark box 2 at the end of the track 1, and a camera 3 for recording the mouse's movement. The track is horizontally arranged with multiple replaceable metal rods, which are composed of any one or two of white and black metal rods. The track, composed of black and white metal rods, is equipped with any one of the metal rods that is energized, and the energized metal rods are not arranged continuously. The mouse is shocked when it steps on an energized metal rod.

[0034] The track also includes multiple indicator lights 4 that are connected to multiple energized metal rods. When a mouse steps on an energized metal rod, the indicator light 4 connected to the energized metal rod flashes.

[0035] The track is made of black plexiglass panels on both sides. The track is 200cm long and 3.5cm wide. The interval between two adjacent metal poles is 1cm.

[0036] This invention also provides a method for detecting learned motor behavior in mice. Using the aforementioned device for detecting learned motor behavior in mice, different tracks are created by changing the metal rods, and mice are trained to reach a dark box via these different tracks to obtain the learned motor behavior abilities of the mice at different stages. The method includes the following steps:

[0037] (1) Set up either black or white metal rods to form the first track. Mice were trained on the first track for 2 to 5 days. The time it took for mice trained on the first track to pass through the first track on their own was measured to obtain the mice’s motor ability.

[0038] (2) Set the ratio of black metal rods to white metal rods to 1:1. Electrify either black or white metal rods to form a second track. Mice trained on the first track are trained on the second track for 2 to 5 days. Test the error rate of mice that have been trained on the second track when they step on the electrified metal rods to obtain the learning ability of mice in goal-oriented behavior.

[0039] (3) Increase the number of unpowered metal rods in the second channel and decrease the number of powered metal rods in the second channel. The ratio of unpowered metal rods to powered metal rods is n:1 to form the third track, where n≥1.5. Mice trained on the second track are trained on the third track for 2-5 days or 10-20 days respectively. The error rate of mice trained on the third track when stepping on powered metal rods is tested. The optimization ability of the learned behavior after 2-5 days of training and the consolidation ability of the learned behavior after 10-20 days of training are obtained respectively.

[0040] (4) Set the unpowered metal rods in the third track to be powered, and the powered metal rods to be unpowered. The ratio of powered metal rods to unpowered metal rods is 1:m to form the fourth track, where m≥1.5. Mice trained on the third track for 2-5 days or 10-20 days are trained on the fourth track for 2-5 days. The error rate of mice stepping on the powered metal rods when they pass through the fourth track after training on the fourth track is tested. The change ability of mice after goal-oriented behavior is changed after 2-5 days of training and the change ability of mice after goal-oriented behavior is fixed are obtained.

[0041] In steps (1) to (4), the training is conducted 5 times a day.

[0042] The ratio of the number of non-energized metal rods to the number of energized metal rods is the ratio of the total number of energized metal rods in the track to the total number of non-energized metal rods in the track. The arrangement of the metal rods in the track is set according to the detection purpose. In step (3), the number and arrangement of energized metal rods are changed, and in step (4), the color of energized metal rods is changed. However, the number of energized metal rods should not be greater than the number of non-energized metal rods, and the number of energized metal rods should not be too small, otherwise the difference will become insignificant. Therefore, preferably, n=2; m=2. Under the requirement of satisfying the ratio of the number of energized metal rods to non-energized metal rods in the track, the energized metal rods are not arranged continuously, that is, two adjacent energized metal rods are non-energized metal rods, and the non-energized metal rods in the track are arranged in various ways, such as discontinuous arrangement or continuous arrangement of 2 to 4 rods.

[0043] Error rate represents the ratio of the number of mice that independently walk across the track to the dark box and step on the electrified metal rods to the total number of electrified metal rods in the track. The higher the ratio, the higher the error rate.

[0044] Example 1

[0045] This embodiment examines the effect of damaged dopaminergic neurons projecting to the substantia nigra pars compacta on the motor ability of mice, including the following steps: a first track is set up with black metal rods, and mice are trained on the first track for 3 days, 5 times a day; the time it takes for the mice trained on the first track to pass through the first track on their own is tested to obtain the mice's motor ability.

[0046] Specifically as follows:

[0047] Day 1: C57BL / 6 mice (25-30g) were anesthetized with isoflurane (4% induction, 1.5% maintenance) and fixed on a stereotaxic instrument. Bilateral microinjections of 6-hydroxydopamine (3mg / ml, 0.4μl) were performed to establish a mouse Parkinson's model.

[0048] Day 14: Open field test to determine whether the mouse Parkinson's disease model was successfully induced;

[0049] Days 15-17: Normal and Parkinson's disease model mice were trained on the first track for 3 consecutive days, 5 times a day.

[0050] Day 18: The effect of damaged dopaminergic neurons projecting to the striatum in the substantia nigra pars compacta on the motor ability of mice was determined. The time taken for normal and Parkinson's disease model mice to traverse the first track independently after training was obtained. The results are as follows: Figure 3 As shown.

[0051] Example 2

[0052] This embodiment examines the impact of selective lesioning of the medial dorsolateral striatum on the learning ability of mice in goal-oriented behavior and the optimization ability of learned behavior, including the following steps:

[0053] (1) Set up a black metal bar to form the first track. The mouse was trained on the first track for 3 days, 5 times a day.

[0054] (2) Set the ratio of black metal rods to white metal rods to 1:1. The white metal rods are electrified to form a second track. Mice trained on the first track are trained on the second track for 3 days, 5 times a day. The error rate of mice stepping on the electrified white metal rods when they pass through the second track is tested to obtain the learning ability of mice in goal-oriented behavior.

[0055] (3) Increase the number of black metal rods in the second channel and decrease the number of white metal rods in the second channel, forming a third track with a black metal rod to white metal rod ratio of 2:1. Mice trained on the second track were trained on the third track for 3 days, 5 times a day. The error rate of mice trained on the third track stepping on the electrified white metal rods when they passed through the third track was tested to obtain the optimization ability of the mice's learned behavior after 3 days of training; Figure 2 The image shows the third track in this embodiment, in which all the white metal poles are arranged discontinuously, some of the black metal poles are arranged discontinuously, and another part of the black metal poles are arranged in 2 to 4 consecutive rows. That is, the black metal poles can be arranged in various ways, such as discontinuous arrangement or continuous arrangement of 2 to 4 rows.

[0056] (4) Set the black metal rods in the third track to be electrified and the white metal rods to be de-electrified. The ratio of black metal rods to white metal rods is 1:2 to form the fourth track. Mice that have been trained on the third track for 3 days are trained on the fourth track for 3 days, with 5 training sessions per day. Test the error rate of mice that have been trained on the fourth track stepping on the electrified black metal rods when they pass through the fourth track on their own. Obtain the ability of mice to learn the behavior optimization after 3 days of training when the goal-oriented behavior is changed.

[0057] Specifically as follows:

[0058] Day 1: C57BL / 6 mice (25-30g) were anesthetized with isoflurane (4% induction, 1.5% maintenance) and fixed on a stereotaxic instrument. Bilateral dorsomedial striatum were micro-injected with AAV9-gad67-caspase-3 (0.2μl) to destroy the dorsomedial striatum (selective apoptosis of dorsomedial striatal GABAergic neurons).

[0059] Days 21–23: Normal mice and mice with dorsolateral striatal lesions were trained 5 times a day on the first track.

[0060] Days 24–26: Normal mice and mice with dorsolateral striatal lesions trained on the first track were trained 5 times a day on the second track.

[0061] Day 27: The error rate of mice stepping on electrified white metal bars while traversing the second track after training was measured to obtain the learning ability of goal-oriented behavior in normal mice and mice with lesions in the medial dorsolateral striatum. The results are as follows: Figure 4 As shown;

[0062] Days 28–30: Normal mice and mice with dorsolateral striatal lesions were trained 5 times a day on the third track;

[0063] Day 31: The error rate of mice stepping on electrified white metal bars while traversing the third track after training was measured to obtain the optimization ability of learned behaviors in normal mice and mice with lesions in the medial dorsolateral striatum. The results are as follows: Figure 5 As shown, the average error rate in the normal group was 17.08 ± 6.42% (mean ± standard deviation, n = 10); the average error rate in the dorsolateral striatum lesion group was 29.38 ± 7.12% (mean ± standard deviation, n = 10).

[0064] Days 32–34: Normal mice and mice with dorsolateral striatal lesions were trained 5 times a day on the fourth track;

[0065] Day 35: The error rate of mice stepping on the electrified black metal bar while traversing the fourth track after training was measured. The optimization ability of normal mice and mice with lesions in the medial dorsolateral striatum was assessed after the goal-directed behavior was altered. Results are as follows: Figure 5 As shown, the average error rate in the normal group was 21.23 ± 8.26% (mean ± standard deviation, n = 10); the average error rate in the dorsolateral striatum lesion group was 32.31 ± 9.17% (mean ± standard deviation, n = 10).

[0066] Example 3

[0067] The experimental objective was the same as in Example 2. By changing the ratio of black and white metal rods in the third and fourth raceways, the ratio of black to white metal rods in the third raceway was adjusted to 3:2, and the ratio of black to white metal rods in the fourth raceway was adjusted to 2:3. The aim was to obtain the effect of the diversity of raceway composition on the ability of selectively damaging the medial dorsolateral striatum to optimize the goal-oriented behavior of mice and the ability to change the learned behavior after optimization.

[0068] Specifically as follows:

[0069] The experimental procedure from day 1 to day 27 was the same as in Example 2. From day 28 to day 30, normal mice and mice with lesions of the medial dorsolateral striatum were trained 5 times a day on the third track.

[0070] Day 31: The error rate of mice stepping on electrified white metal bars while traversing the third track after training was measured to obtain the optimization ability of learned behaviors in normal mice and mice with lesions in the medial dorsolateral striatum. The results are as follows: Figure 6 As shown, the average error rate in the normal group was 19.75 ± 7.07% (mean ± standard deviation, n = 10); the average error rate in the dorsolateral striatum lesion group was 33.38 ± 6.43% (mean ± standard deviation, n = 10).

[0071] Days 32–34: Normal mice and mice with dorsolateral striatal lesions were trained 5 times a day on the fourth track;

[0072] Day 35: The error rate of mice stepping on the electrified black metal bar while traversing the fourth track after training was measured. The improved behavior of normal mice and mice with lesions in the medial dorsolateral striatum was assessed after the goal-oriented behavior was altered. Results are as follows: Figure 6 As shown, the average error rate in the normal group was 24.38 ± 5.69% (mean ± standard deviation, n = 10); the average error rate in the dorsolateral striatum lesion group was 35.88 ± 7.88% (mean ± standard deviation, n = 10).

[0073] Example 4

[0074] This embodiment examines the ability to solidify and alter learned behaviors in mice by selectively inhibiting the dorsolateral striatum, including the following steps:

[0075] (1) Set up a black metal bar to form the first track. The mouse was trained on the first track for 3 days, 5 times a day.

[0076] (2) Set the ratio of black metal rods to white metal rods to 1:1. The white metal rods are electrified to form a second track. Mice trained on the first track are trained on the second track for 3 days, 5 times a day. The error rate of mice stepping on the electrified white metal rods when they pass through the second track is tested to obtain the learning ability of mice in goal-oriented behavior.

[0077] (3) Increase the number of black metal rods in the second channel and decrease the number of white metal rods in the second channel. The ratio of black metal rods to white metal rods is 2:1 to form the third track. Mice trained on the second track are trained on the third track for 10 days, 5 times a day. The error rate of mice trained on the third track stepping on the electrified white metal rods when they pass through the third track is tested to obtain the solidification ability of the learned behavior after 10 days of training.

[0078] (4) In the third track, the black metal rods are turned on and the white metal rods are turned off. The ratio of black metal rods to white metal rods is 1:2 to form the fourth track. Mice that have been trained in the third track for 10 days are trained in the fourth track for 3 days, 5 times a day. The error rate of mice that step on the on-powered black metal rods when they pass through the fourth track after training in the fourth track is tested to obtain the ability of mice to change their learned behavior after the goal-oriented behavior is changed after 3 days of training.

[0079] Specifically as follows:

[0080] Day 1: C57BL / 6 mice (25-30g) were anesthetized with isoflurane (4% induction, 1.5% maintenance) and fixed on a stereotaxic instrument. AAV9-gad67-caspase-3 (0.2μl) was injected into the bilateral dorsolateral striatum to destroy the dorsolateral striatum (selective apoptosis of dorsolateral striatal GABAergic neurons).

[0081] Days 21–23: Normal mice and mice with dorsolateral striatal lesions were trained 5 times a day on the first track;

[0082] Days 24–26: Normal mice and mice with dorsolateral striatal lesions were trained 5 times a day on the second track;

[0083] Days 27–36: Normal and dorsolateral striatal lesion mice were trained 5 times a day on the third track;

[0084] Day 37: The error rate of mice stepping on electrified white metal bars while traversing the third track after training was measured to obtain the consolidation ability of learned behaviors in normal mice and mice with lesions in the dorsolateral striatum. The results are as follows: Figure 7 As shown, the mean error rate of the normal group was 14.15 ± 4.85% (mean ± standard deviation, n = 10); the mean error rate of the dorsolateral striatum lesion group was 20.77 ± 6.37% (mean ± standard deviation, n = 10).

[0085] Days 38–40: Normal and dorsolateral striatal lesion mice were trained 5 times a day on the fourth track;

[0086] Day 41: The error rate of mice stepping on the electrified black metal bar while traversing the fourth track after training was measured. The ability of normal mice and mice with dorsolateral striatal lesions to change learned behavior after the goal-directed behavior was altered was obtained. Results are as follows: Figure 7 As shown, the average error rate in the normal group was 30.62 ± 7.34% (mean ± standard deviation, n = 10); the average error rate in the dorsolateral striatum lesion group was 22 ± 6.65% (mean ± standard deviation, n = 10).

[0087] Example 5

[0088] The experimental objective was the same as in Example 4. By changing the ratio of black and white metal rods in the third and fourth raceways, the ratio of black to white metal rods in the third raceway was adjusted to 3:2, and the ratio of black to white metal rods in the fourth raceway was adjusted to 2:3. The aim was to obtain the effect of the diversity of raceway composition on the solidification of target-oriented behavior in mice by selectively damaging the dorsolateral striatum and the ability to change it after solidification.

[0089] Specifically as follows:

[0090] The experimental procedure from day 1 to day 26 was the same as in Example 4. From day 27 to day 36, normal mice and mice with dorsolateral striatal lesions were trained 5 times a day on the third track.

[0091] Day 37: The error rate of mice stepping on electrified white metal bars while traversing the third track after training was measured to obtain the consolidation ability of learned behaviors in normal mice and mice with lesions in the dorsolateral striatum. The results are as follows: Figure 8 As shown, the average error rate in the normal group was 16.75 ± 4.65% (mean ± standard deviation, n = 10); the average error rate in the dorsolateral striatum lesion group was 24.25 ± 5.69% (mean ± standard deviation, n = 10).

[0092] Days 38–40: Normal and dorsolateral striatal lesion mice were trained 5 times a day on the fourth track;

[0093] Day 41: The error rate of mice stepping on the electrified black metal bar while traversing the fourth track after training was measured. The ability of normal mice and mice with dorsolateral striatal lesions to change learned behavior after the goal-directed behavior was altered was also assessed. Results are as follows: Figure 8 As shown, the average error rate in the normal group was 32.88 ± 6.87% (mean ± standard deviation, n = 10); the average error rate in the dorsolateral striatum lesion group was 23.5 ± 7.02% (mean ± standard deviation, n = 10).

[0094] from Figure 3As can be seen, the time taken for the Parkinson's disease model mice to cross the first track was significantly increased in Example 1, indicating a significant reduction in motor ability in the Parkinson's disease model mice; from Figure 4 It is evident that lesions in the medial dorsolateral striatum in Example 2 increased the error rate of mice stepping on the second track, indicating that lesions in the medial dorsolateral striatum hindered the learning ability of mice to perform goal-oriented behaviors; from Figures 5-6 As can be seen, damage to the medial dorsolateral striatum in Examples 2 and 3 increased the error rate of mice stepping on the third track, indicating that damage to the medial striatum hindered the mice's ability to optimize learned behavior; the error rate of normal mice stepping on the third and fourth tracks in Examples 2 and 3 did not change significantly, indicating that mice had a high adaptability to new things during the establishment stage of learned behavior; while the error rate of mice with damage to the medial dorsolateral striatum stepping on the fourth track increased significantly, indicating that damage to the medial dorsolateral striatum hindered the adaptability of mice's learned behavior.

[0095] from Figures 7-8 As can be seen, damage to the dorsolateral striatum in Examples 4 and 5 increased the error rate of mice stepping on the third track, indicating that damage to the dorsolateral striatum hindered the mice's ability to solidify learned behaviors; in Example 3, the error rate of normal mice stepping on the fourth track was significantly higher than that of the third track, indicating that mice had lower adaptability to new things during the solidification stage of learned behaviors; while the error rate of mice with dorsolateral striatum damage stepping on the fourth track was significantly reduced, indicating that damage to the dorsolateral striatum hindered the solidification of learned behaviors in mice.

Claims

1. A method for detecting the motor learning ability of mice, characterized in that, A device for detecting mouse motor learning behavior is used. The device includes a track (1) for mouse movement, a dark box (2) at the end of the track (1), and a camera (3) for recording the mouse movement process. The track is horizontally arranged with multiple replaceable metal rods, which are composed of any one or two of white and black metal rods. The track composed of black and white metal rods is equipped with any one of the metal rods being energized, and the energized metal rods are not continuous. The mouse is shocked when it steps on an energized metal rod. By changing the metal rods to form different tracks, the mouse is trained to reach the dark box through different tracks, and the motor learning behavior ability of the mouse at different stages is obtained. The device includes the following steps: Step (1): Set up either a black metal bar or a white metal bar to form the first track, and train the mice on the first track for 2 to 5 days; Step (2): Set the ratio of black metal rods to white metal rods to 1:

1. Electricize either black or white metal rods to form a second track. Mice trained on the first track are trained on the second track for 2 to 5 days. Step (3): Increase the number of unpowered metal rods in the second channel and decrease the number of powered metal rods in the second channel. The ratio of unpowered metal rods to powered metal rods is n:1 to form the third track, where n≥1.

5. Mice trained in the second track are trained in the third track for 2-5 days or 10-20 days respectively. Step (4): Set the unpowered metal rods in the third track to be powered, and the powered metal rods to be unpowered. The ratio of the number of powered metal rods to the number of unpowered metal rods is 1:m to form the fourth track, where m≥1.

5. Mice that have been trained in the third track for 2-5 days or 10-20 days are trained in the fourth track for 2-5 days. Step (1) also includes testing the time it takes for the mice trained on the first track to pass through the first track on their own, in order to obtain the mice’s motor ability; Step (2) also includes testing the error rate of mice that have been trained on the second track stepping on the electrified metal rods when they pass through the second track on their own, in order to obtain the learning ability of mice in goal-oriented behavior. Step (3) also includes testing the error rate of mice stepping on the electrified metal rods when they pass through the third track after training in the third track, and obtaining the optimization ability of the learned behavior after 2 to 5 days of training, and the consolidation ability of the learned behavior after 10 to 20 days of training. Step (4) also includes testing the error rate of mice stepping on the electrified metal rods when they pass through the fourth track after training in the fourth track, and obtaining the ability of mice to change their learned behavior after the goal-oriented behavior was changed after 2 to 5 days of training, as well as the ability of mice to change their learned behavior after the goal-oriented behavior was changed after 2 to 5 days of training.

2. The method according to claim 1, characterized in that, The running track has black plexiglass panels on both sides, and is 200 cm long and 3.5 cm wide. The interval between two adjacent metal poles is 1 cm.

3. The method according to claim 1, characterized in that, The track also includes multiple indicator lights (4) connected to multiple energized metal rods. When a mouse steps on an energized metal rod, the indicator light (4) connected to the energized metal rod flashes.

4. The method according to claim 1, characterized in that, In steps (1) to (4), the training is conducted 5 times a day.

Images