Method for rapid germination acceleration of Euscaphis konishii seeds and improvement of seed germination rate

By rinsing and soaking the seeds of round-tooth wild cypress seeds, combined with sand storage technology, the problems of long germination cycle and low germination rate of round-tooth wild cypress seeds are solved, and seed germination with short-cycle and high germination rate are achieved.

CN116649040BActive Publication Date: 2025-07-11JIANGXI AGRICULTURAL UNIVERSITY
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Patent Information

Application Number
CN202310697912.8
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2023-06-13
Publication Date
2025-07-11
Estimated Expiration
2043-06-13

AI Technical Summary

Technical Problem

The prior art cannot simultaneously realize the problem of short germination cycle and high germination rate of round-tooth wild cypress seeds.

Method used

By selecting 10-15-year-old mother tree seeds, combining seed seeds with water soaked seeds at 50-55°C before sowing, the specific steps include rinsing, sand storage and seed soaking treatment.

Benefits of technology

The germination cycle was shortened to 120 to 150 days, and the germination rate remained at 70 to 75%, significantly improving the seed germination rate.

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Abstract

The present invention relates to the technical field of seed germination treatment, and discloses a method for rapidly accelerating the germination of Euscaphis konishii seeds and improving the seed germination rate, which includes the following steps: S1. Selecting and collecting seeds; S2. Cleaning the seeds; S3. Sprinkling the seeds: rinsing the target seeds prepared in S2 with water to obtain the rinsed seeds; S4. Stratification: subjecting the rinsed seeds prepared in S3 to sand storage and natural stratification for 120 - 150 days to obtain the stratified seeds, wherein the water content of the sand is 4 - 15%; S5. Soaking the seeds: soaking the stratified seeds in water at 50 - 55°C, and after cooling, continuing to soak for 24 - 36 hours before sowing. By using the seeds of 10 - 15-year-old mother trees, sprinkling the seeds before stratification and soaking the seeds in water at 50 - 55°C before sowing, the germination acceleration period of Euscaphis konishii seeds is shortened to 120 - 150 days, and the germination rate remains at 70 - 75%.
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Description

Technical Field

[0001] The present invention belongs to the technical field of seed germination treatment, and particularly relates to a method for rapidly accelerating the germination of Euscaphis konishii seeds and improving the seed germination rate. Background Art

[0002] Euscaphis konishii Hayata belongs to the genus Euscaphis of the family Staphyleaceae, and is an evergreen small tree unique to China. It is a rare medicinal and ornamental tree species. Its fruits are bright red and shiny, and the fruiting period lasts for more than 7 months, which is highly praised by horticulturalists. Its roots or root bark, flowers, and dried fruits are good Chinese herbal medicines, and also have high potential for the development of anti-cancer drugs. The seeds contain 25% - 30% oil, which can be used both medicinally and as industrial oil. Vigorously developing and utilizing, and promoting the planting of this native tree species resource can not only meet the needs of garden construction, but also be used as a medicinal plant and oil crop, which is of extremely important significance for promoting the local economic development.

[0003] The pith of Euscaphis konishii is relatively large, and it is not suitable to be propagated by cutting and grafting methods. Therefore, in production practice, sowing and seedling raising is still a common method for breeding Euscaphis konishii seedlings. However, the seeds of Euscaphis konishii have deep dormancy, the germination time span is long, and it is difficult to break dormancy. The span from seed collection to sowing and emergence is 3 years, and the emergence rate is low. In order to obtain a higher seed germination rate and more robust seedlings, the seeds must be accelerated in germination.

[0004] The existing methods for accelerating the germination of Euscaphis konishii seeds are as follows: 1. After the seeds are picked, they are treated by cold stratification with clean river sand at 4°C. When stratification reaches 120 days, they are taken out and soaked in warm water at 60°C for 24 hours before sowing, and then naturally cooled. The peak germination rate occurs at 120 days of stratification. Reference: Zhang Limei, etc. Physiological and biochemical changes of Euscaphis konishii seeds during low-temperature stratification [J]. Seed, 2015, 34(7): 37 - 40. However, the germination rate of Euscaphis konishii seeds by this method is only 48%. If the stratification time exceeds 120 days, the germination rate will decrease. 2. At the bottom of a wooden box or on the ground in a basement, first lay a layer of river sand about 5 - 10 cm thick with a moisture content of 4 - 5%, then sprinkle a layer of seeds about 2 cm thick, and then sprinkle a layer of river sand about 5 cm thick. The seeds are sprinkled in turn, and the top layer of river sand is 5 cm thick. The stratification height does not exceed 50 cm. During the sand storage process, except in winter, every 7 - 15 days, remove the obviously bad seeds, adjust the moisture content of the sand to maintain it at 4 - 5%, and then store it in the original way. After 360 - 450 days, the seeds begin to crack. When the number of cracked seeds reaches about 10%, sieve them out for sowing, and the germination rate reaches 75%. Reference: Zou Shuangquan (2018). Research on Euscaphis konishii. Science Press, 2018: 31 - 37. Although this method has a relatively high germination rate, the germination acceleration period is still too long. Summary of the Invention

[0005] The present invention discloses a method for rapidly germinating Euscaphis konishii seeds and improving the seed germination rate, which solves the problem that in the prior art, when germinating Euscaphis konishii seeds, it is impossible to achieve both a short germination period and a high germination rate at the same time.

[0006] A method for rapidly germinating Euscaphis konishii seeds and improving the seed germination rate specifically includes the following steps:

[0007] S1. Seed selection and collection: Select mother trees aged 10 - 15 years, and collect seeds in October - November when the pericarp cracks and black seeds are exposed to obtain follicles.

[0008] S2. Seed cleaning: Remove the pericarp and impurities from the follicles collected in S1 to obtain target seeds.

[0009] S3. Seed rinsing: Rinse the target seeds prepared in S2 with water at a flow rate of 0.8 - 1.0 m / s to obtain rinsed seeds.

[0010] S4. Stratification: Subject the rinsed seeds prepared in S3 to sand storage for natural stratification for 120 - 150 days to obtain stratified seeds; wherein, the water content of the sand is 4 - 15%.

[0011] S5. Seed soaking: Soak the stratified seeds in water at 50 - 55 °C, and after cooling, continue to soak for 24 - 36 h and then sow.

[0012] Preferably, the specific method of rinsing in S3 is: Rinse for 2 - 2.5 h each time, 3 times a day, for 3 days.

[0013] Preferably, the interval between two rinses is 2 h.

[0014] Preferably, the specific method of stratification in S4 is: First, lay a 10 - cm - thick layer of gravel, then lay a 10 - cm - thick layer of sand with a water content of 4 - 15%, then mix the seeds with sand with a water content of 4 - 15% and pile them on the sand layer, and then cover with a 10 - cm - thick layer of sand with a water content of 4 - 15%, and cover the topmost layer with 5 - 7 cm of yellow - heart soil.

[0015] Preferably, the seeds are mixed with sand with a water content of 4 - 15% at a volume ratio of 1:3.

[0016] Preferably, the soil used for the topmost layer is yellow - heart soil.

[0017] Preferably, when soaking seeds in S5, the volume ratio of water to the stratified seeds is not less than 25:1.

[0018] Preferably, when cleaning seeds in S2, first spread the harvested seeds in a cool and ventilated place to allow the follicles to naturally crack and the seeds to scatter.

[0019] Preferably, during the impurity removal in S2, water selection is used to purify the seeds.

[0020] Preferably, the water selection method is to place the seeds in water at 20-30°C to remove impurities and empty seeds.

[0021] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0022] By using the method of selecting seeds from 10-15-year-old mother trees, presoaking the seeds before stratification, and soaking the seeds in water at 50-55°C before sowing, the germination period of Euscaphis konishii seeds is shortened to 120-150 days, and the germination rate remains at 70-75%. Description of the Drawings

[0023] Figure 1 It shows the change of the seed germination rate with the sowing time when the seeds of Euscaphis konishii are stratified for 120 days by the method for rapid germination and improving the seed germination rate of Euscaphis konishii seeds provided by the present invention. Detailed Embodiments

[0024] The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the specific embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without creative efforts shall fall within the protection scope of the present invention.

[0025] The methods described in the embodiments of the present invention are all conventional methods unless otherwise specified. The materials, reagents, etc. used can be obtained from commercial channels unless otherwise specified.

[0026] Embodiment 1

[0027] The method for rapid germination and improving the seed germination rate of Euscaphis konishii seeds specifically includes the following steps:

[0028] S1. Seed selection and collection: Select a 10-year-old mother tree with strong growth, and collect the seeds in October when the pericarp cracks and black seeds are exposed to obtain follicles.

[0029] S2. Seed purification: Spread the follicles collected in S1 in a cool and ventilated place, and let the follicles naturally crack to release the seeds. Place the released seeds in water at 20°C to remove impurities and empty seeds to obtain the target seeds.

[0030] S3. Presoaking: Rinse the target seeds prepared in S2 with tap water at a flow rate of 0.8 m / s, rinse for 2 hours each time, 3 times a day, with a 2-hour interval each time, and rinse for 3 days to obtain the rinsed seeds.

[0031] S4. Stratification: The washed seeds prepared in S3 are subjected to natural stratification in sand until the 120th day of stratification to obtain stratified seeds. The specific method of stratification is as follows: First, spread 10 cm thick gravel, then lay a 10 cm thick sand layer with a water content of 4%, then mix the seeds with sand with a water content of 4% in a volume ratio of 1:3 and pile them on the sand layer, then cover with 10 cm thick sand with a water content of 4%, and cover the top layer with 5 cm thick yellow heart soil.

[0032] S5. Seed soaking: Soak the stratified seeds in water with an initial temperature of 50 °C, with a volume ratio of water to seeds of 25:1. After naturally cooling to room temperature, soak for 24 h continuously and then proceed with sowing.

[0033] Example 2

[0034] Method for rapid germination promotion and seed germination rate improvement of Euscaphis konishii seeds, specifically including the following steps:

[0035] S1. Seed selection and collection: Select a 12-year-old mother tree with strong growth. In October, when the pericarp cracks and black seeds are exposed, collect the seeds to obtain follicles.

[0036] S2. Seed cleaning: Spread the follicles collected in S1 in a cool and ventilated place, let the follicles naturally crack and the seeds scatter, place the scattered seeds in water at 25 °C, remove impurities and empty seeds to obtain target seeds.

[0037] S3. Seed rinsing: Rinse the target seeds prepared in S2 with tap water at a flow rate of 0.9 m / s, rinse for 2 h each time, 3 times a day, and rinse for 3 days to obtain rinsed seeds.

[0038] S4. Stratification: The rinsed seeds prepared in S3 are subjected to natural stratification in sand until the 130th day of stratification to obtain stratified seeds. The specific method of stratification is as follows: First, spread 10 cm thick gravel, then lay a 10 cm thick sand layer with a water content of 10%, then mix the seeds with sand with a water content of 10% in a volume ratio of 1:3 and pile them on the sand layer, then cover with 10 cm thick sand with a water content of 10%, and cover the top layer with 6 cm thick yellow heart soil.

[0039] S5. Seed soaking: Soak the stratified seeds in water with an initial temperature of 53 °C, with a volume ratio of water to seeds of 30:1. After naturally cooling to room temperature, soak for 30 h continuously and then proceed with sowing.

[0040] Example 3

[0041] Method for rapid germination promotion and seed germination rate improvement of Euscaphis konishii seeds, specifically including the following steps:

[0042] S1. Seed selection and collection: Select a 15-year-old mother tree with strong growth. In October, when the pericarp cracks and black seeds are exposed, collect the seeds to obtain follicles.

[0043] S2. Seed cleaning: Spread the follicles harvested in S1 in a cool and ventilated place. After the follicles naturally split and the seeds scatter, place the scattered seeds in water at 30°C, remove impurities and empty seeds to obtain the target seeds.

[0044] S3. Seed rinsing: Rinse the target seeds prepared in S2 with tap water at a flow rate of 1.0 m / s. Rinse for 2 hours each time, 3 times a day, and rinse for 3 days to obtain the rinsed seeds.

[0045] S4. Stratification: Subject the rinsed seeds prepared in S3 to natural stratification in sand. By the 150th day of stratification, obtain the stratified seeds. The specific method of stratification is to first lay a 10-cm-thick layer of gravel, then lay a 10-cm-thick layer of sand with a water content of 15%. Then mix the seeds with sand with a water content of 15% in a volume ratio of 1:3 and pile them on the sand layer. Then cover with a 10-cm-thick layer of sand with a water content of 15%, and cover the topmost layer with 7-cm-thick yellow heart soil.

[0046] S5. Seed soaking: Soak the stratified seeds in water at an initial temperature of 55°C. The volume ratio of water to seeds is 35:1. After naturally cooling to room temperature, continue to soak for 36 hours and then proceed with sowing.

[0047] Comparative Example 1

[0048] Method for rapid germination promotion and germination rate improvement of Euscaphis konishii seeds, specifically including the following steps:

[0049] S1. Seed selection and collection: Select 20-year-old female trees with strong growth, high fruit yield, bright and colorful fruits, and a long fruiting period. In October, when the fruit peel splits and black seeds are exposed, collect the fruits to obtain follicles.

[0050] S2. Seed cleaning: Spread the follicles harvested in S1 in a cool and ventilated place. After the follicles naturally split and the seeds scatter, place the scattered seeds in water at 30°C, remove impurities and empty seeds to obtain the target seeds.

[0051] S3. Seed rinsing: Rinse the target seeds prepared in S2 with tap water at a flow rate of 1.0 m / s. Rinse for 2 hours each time, 3 times a day, and rinse for 3 days to obtain the rinsed seeds.

[0052] S4. Stratification: Subject the rinsed seeds prepared in S3 to natural stratification in sand. By the 120th day of stratification, obtain the stratified seeds. The specific method of stratification is to first lay a 10-cm-thick layer of gravel, then lay a 10-cm-thick layer of sand with a water content of 15%. Then mix the seeds with sand with a water content of 15% in a volume ratio of 1:3 and pile them on the sand layer. Then cover with a 10-cm-thick layer of sand with a water content of 15%, and cover the topmost layer with 7-cm-thick yellow heart soil.

[0053] S5. Seed soaking: Soak the stratified seeds in water at an initial temperature of 55°C, with a volume ratio of water to seeds of 25:1. After natural cooling to room temperature, continue soaking for 36 hours and then proceed with sowing.

[0054] Comparative Example 2

[0055] A method for rapid germination acceleration and seed germination rate improvement of Euscaphis konishii seeds, specifically including the following steps:

[0056] S1. Seed selection and collection: Select 15-year-old mother trees with strong growth. In October, when the pericarp cracks and black seeds are exposed, collect the seeds to obtain follicles.

[0057] S2. Seed cleaning: Spread the follicles harvested in S1 in a cool and ventilated place. After the follicles naturally crack and the seeds are scattered, place the scattered seeds in water at 30°C to remove impurities and empty seeds, obtaining the target seeds.

[0058] S3. Stratification: Subject the target seeds obtained in S2 to natural stratification in sand. By the 120th day of stratification, obtain the stratified seeds. The specific method of stratification is to first lay a 10-cm-thick layer of gravel, then pad a 10-cm-thick layer of sand with a water content of 15%. Then, mix the seeds with sand with a water content of 15% in a volume ratio of 1:3 and pile them on the sand layer, and then cover with a 10-cm-thick layer of sand with a water content of 15%. The top layer is covered with a 7-cm-thick layer of yellow heart soil.

[0059] S4. Seed soaking: Soak the stratified seeds in water at an initial temperature of 55°C, with a volume ratio of water to seeds of 25:1. After natural cooling to room temperature, continue soaking for 36 hours and then proceed with sowing.

[0060] Comparative Example 3

[0061] A method for rapid germination acceleration and seed germination rate improvement of Euscaphis konishii seeds, specifically including the following steps:

[0062] S1. Seed selection and collection: Select 15-year-old mother trees with strong growth. In October, when the pericarp cracks and black seeds are exposed, collect the seeds to obtain follicles.

[0063] S2. Seed cleaning: Spread the follicles harvested in S1 in a cool and ventilated place. After the follicles naturally crack and the seeds are scattered, place the scattered seeds in water at 30°C to remove impurities and empty seeds, obtaining the target seeds.

[0064] S3. Spraying: Spray the target seeds prepared in S2 with tap water at a flow rate of 1.0 m / s, spray for 2 hours each time, 3 times a day, and rinse for 3 days to obtain the rinsed seeds.

[0065] S4. Seed soaking: Soak the rinsed seeds in water at an initial temperature of 55°C, with a volume ratio of water to seeds of 25:1. After natural cooling to room temperature, continue soaking for 36 hours and then proceed with sowing.

[0066] Comparative Example 4

[0067] Method for rapid germination acceleration of Euscaphis konishii seeds and improvement of seed germination rate, specifically comprising the following steps:

[0068] S1. Seed selection and collection: Select 15-year-old mother trees with strong growth. In October, when the pericarp cracks and black seeds are exposed, collect the seeds to obtain follicles.

[0069] S2. Seed cleaning: Spread the follicles harvested in S1 in a cool and ventilated place. After the follicles naturally crack and the seeds scatter, place the scattered seeds in water at 30°C, remove impurities and empty and shriveled seeds to obtain target seeds.

[0070] S3. Seed rinsing: Rinse the target seeds prepared in S2 with tap water at a flow rate of 1.0 m / s. Rinse for 2 h each time, 3 times a day, and rinse for 3 days to obtain rinsed seeds.

[0071] S4. Stratification: Subject the rinsed seeds prepared in S3 to sand storage and natural stratification. By the 120th day of stratification, obtain stratified seeds for sowing. The specific method of stratification is to first lay a 10-cm-thick layer of gravel, then pad a 10-cm-thick layer of sand with a water content of 15%. Then mix the seeds with sand with a water content of 15% in a volume ratio of 1:3 and stack them on the sand layer, and then cover with a 10-cm-thick layer of sand with a water content of 15%. The top layer is covered with 7-cm-thick yellow core soil. Take out the stratified seeds and directly sow them.

[0072] Comparative Example 5

[0073] At the bottom of a wooden box or on the ground in a basement, first place a layer of river sand about 5 - 10 cm thick with a moisture content of 4 - 5%. Then sprinkle a layer of seeds about 2 cm thick, and then sprinkle a layer of river sand about 5 cm thick. Finish spreading the seeds in sequence. The top layer of river sand is 5 cm thick, and the stratification height does not exceed 50 cm. During the sand storage process, except in winter, remove the obviously damaged seeds every 7 - 15 days, adjust the moisture content of the sand to maintain it at 4 - 5%, and then store it as it is. After 120 days, sieve out the seeds for sowing.

[0074] Comparative Example 6

[0075] At the bottom of a wooden box or on the ground in a basement, first place a layer of river sand about 5 - 10 cm thick with a moisture content of 4 - 5%. Then sprinkle a layer of seeds about 2 cm thick, and then sprinkle a layer of river sand about 5 cm thick. Finish spreading the seeds in sequence. The top layer of river sand is 5 cm thick, and the stratification height does not exceed 50 cm. During the sand storage process, except in winter, remove the obviously damaged seeds every 7 - 15 days, adjust the moisture content of the sand to maintain it at 4 - 5%, and then store it as it is. After 360 - 450 days, the seeds begin to crack. When the number of cracked seeds reaches about 10%, sieve out the seeds for sowing.

[0076] Comparative Example 7

[0077] Method for rapid germination acceleration of Euscaphis konishii seeds and improvement of seed germination rate, specifically including the following steps:

[0078] S1. Seed selection and collection: Select 5-year-old mother trees with strong growth. In October, when the pericarp cracks and black seeds are exposed, collect the seeds to obtain follicles.

[0079] S2. Seed cleaning: Spread the follicles harvested in S1 in a cool and ventilated place to allow the seeds to scatter after the follicles naturally crack. Place the scattered seeds in water at 30°C, remove impurities and empty seeds to obtain target seeds.

[0080] S3. Seed rinsing: Rinse the target seeds prepared in S2 with tap water at a flow rate of 1.0 m / s, rinse for 2 hours each time, 3 times a day, and rinse for 3 days to obtain rinsed seeds.

[0081] S4. Stratification: Carry out sand storage natural stratification on the rinsed seeds prepared in S3. By the 120th day of stratification, obtain stratified seeds; the specific method of stratification is to first spread 10 cm thick gravel, then lay a 10 cm thick sand layer with a water content of 15%, then mix the seeds with sand with a water content of 15% at a volume ratio of 1:3 and pile them on the sand layer, and then cover with 10 cm thick sand with a water content of 15%. The top layer is covered with 7 cm thick yellow heart soil.

[0082] S5. Seed soaking: Soak the stratified seeds in water at an initial temperature of 55°C, with a volume ratio of water to seeds of 25:1. After naturally cooling to room temperature, continuously soak for 36 hours and then carry out sowing.

[0083] Comparative Example 8

[0084] Method for rapid germination acceleration of Euscaphis konishii seeds and improvement of seed germination rate, specifically including the following steps:

[0085] S1. Seed selection and collection: Select 15-year-old mother trees with strong growth. In October, when the pericarp cracks and black seeds are exposed, collect the seeds to obtain follicles.

[0086] S2. Seed cleaning: Spread the follicles harvested in S1 in a cool and ventilated place to allow the seeds to scatter after the follicles naturally crack. Place the scattered seeds in water at 30°C, remove impurities and empty seeds to obtain target seeds.

[0087] S3. Seed rinsing: Rinse the target seeds prepared in S2 with tap water at a flow rate of 1.0 m / s, rinse for 2 hours each time, 3 times a day, and rinse for 3 days to obtain rinsed seeds.

[0088] S4. Stratification: The rinsed seeds prepared in S3 are subjected to natural stratification in sand until the 90th day of stratification to obtain stratified seeds. The specific method of stratification is as follows: First, spread gravel with a thickness of 10 cm, then lay a sand layer with a thickness of 10 cm and a water content of 15%. Then, mix the seeds with sand with a water content of 15% at a volume ratio of 1:3 and stack them on the sand layer. Next, cover them with 10 cm thick sand with a water content of 15%, and cover the top layer with 7 cm thick yellow core soil.

[0089] S5. Seed soaking: Soak the stratified seeds in water with an initial temperature of 55 °C, with a volume ratio of water to seeds of 25:1. After naturally cooling to room temperature, soak the seeds continuously for 36 h and then proceed with sowing.

[0090] Table 1 Stratification time and germination rate of Euscaphis konishii seeds under different germination promotion methods

[0091]

[0092]

[0093] From the above data, it can be seen that in Examples 1 to 3, by collecting seeds from mother trees aged 10 to 15 years, rinsing before stratification, stratifying for 120 to 150 days, and soaking the seeds in water at 50 to 55 °C after stratification, the germination rate can reach 70 to 75%, which is much higher than the germination rates in Comparative Examples 1, 2, 4, and 7 where seeds are collected from 5-year-old or 20-year-old trees, or seeds are collected from 10 to 15-year-old trees but not rinsed or not soaked before sowing. Although Comparative Example 3 adopted the steps of collecting seeds from mother trees aged 10 to 15 years, rinsing, and soaking, but without stratification, so the germination rate is zero. Although Comparative Example 6 can also make the germination rate of Euscaphis konishii seeds reach more than 70%, this method requires a stratification time of 450 days (the germination rate is only 5% when stratified for 120 days, see Comparative Example 5), which is much longer than the 120 to 150 days in the examples. Comparative Example 8 has the same method as Examples 1 to 3, but the stratification time is too short and the germination rate is much lower than that of Examples 1 to 3. Therefore, compared with other methods, the method provided by the present invention is an effective method that can not only shorten the germination promotion time of Euscaphis konishii seeds but also ensure a high germination rate.

[0094] Although the preferred embodiments of the present invention have been described, those skilled in the art can make additional changes and modifications once they know the basic creative concept. Therefore, the appended claims are intended to be construed to include the preferred embodiments as well as all changes and modifications falling within the scope of the present invention.

[0095] Obviously, those skilled in the art can make various changes and modifications to the present invention without departing from the spirit and scope of the present invention. Thus, if these modifications and variations of the present invention fall within the scope of the claims of the present invention and their equivalent technologies, the present invention is also intended to include these modifications and variations.

Claims

1. A method for rapid germination acceleration and improvement of seed germination rate of Euscaphis konishii, characterized in that Specifically, it includes the following steps: S1. Seed selection and collection: Select mother trees that are 14 - 16 years old. In October - November, when the pericarp cracks and black seeds are exposed, collect the follicles. S2. Seed cleaning: Spread the follicles collected in S1 in a cool and ventilated place. After the follicles naturally crack and the seeds are scattered, remove the peel and impurities to obtain the target seeds. S3. Seed rinsing: Rinse the target seeds prepared in S2 with water for 2 - 2.5 hours, three times a day, for 3 days, with a flow rate of 0.8 - 1.0 m / s, to obtain the rinsed seeds. S4. Stratification: The specific method is as follows: First, spread 10 cm thick gravel, then lay a 10 cm thick sand layer with a water content of 14 - 16%. Then mix the rinsed seeds with sand with a water content of 14 - 16% and pile them on the sand layer. Then cover with 10 cm thick sand with a water content of 14 - 16%. The top layer is covered with 5 - 7 cm thick soil for stratification for 120 - 150 days to obtain the stratified seeds. S5. Seed soaking: Soak the stratified seeds in water at 54 - 56 °C. After cooling, continue to soak for 35 - 37 hours and then proceed to sowing.

2. The method for rapid germination acceleration of Euscaphis konishii seeds and improvement of seed germination rate according to claim 1, characterized in that, The rinsed seeds are mixed with sand with a water content of 14 - 16% at a volume ratio of 1:

3.

3. The method for rapid germination acceleration and germination rate improvement of Euscaphis konishii seeds according to claim 1, characterized in that The soil used in the top layer in S4 is yellow - heart soil.

4. The method for rapid germination acceleration and germination rate improvement of Euscaphis konishii seeds according to claim 1, characterized in that, When soaking seeds in S5, the volume ratio of water to the stratified seeds is not less than 25:

1.

5. The method for rapid germination acceleration and germination rate improvement of Euscaphis konishii seeds according to claim 1, characterized in that, When cleaning impurities in S2, the seeds are cleaned by water - selection method.

6. The method for rapid germination acceleration and germination rate improvement of Euscaphis konishii seeds according to claim 5, characterized in that, The water - selection method is to place the seeds in water at 20 - 30 °C to remove sundries and empty and shrunken seeds.

Citation Information

Patent Citations

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