Cosmetic composition comprising a mixed extract of cotton, balsam pear, hibiscus, and hollyhock as an effective ingredient, and a preparation method thereof

Abstract

The present invention relates to a cosmetic composition for achieving a moisturizing effect by containing a mixed extract of cotton, balsam pear, madder flower, and hibiscus as an effective ingredient, and a preparation method thereof.

Description

Technical Field

[0001] This invention relates to a cosmetic composition and its preparation method, and more specifically, to a cosmetic composition and its preparation method that achieves moisturizing effects by using a mixed extract of cotton, bitter melon, hibiscus and hollyhock as an active ingredient. Background Technology

[0002] The skin is the main barrier against the external environment, playing a vital role in protecting our bodies from damage caused by wounds, infections, moisture loss, and ultraviolet (UV) radiation. UV radiation is one of the main causes of skin aging by damaging the skin, and extensive research is being conducted in the field of dermatology to investigate the relationship between UV radiation and the skin.

[0003] Skin hydration acts as a barrier, protecting the body from various external stimuli while preventing moisture loss from the skin's interior. To achieve this moisture barrier function, the epidermis undergoes a differentiation process to retain moisture and develop into a thin stratum corneum. Structurally, the cells of the stratum corneum act like bricks, while the intercellular lipids of the corneocytes act like mortar, thus forming the skin barrier.

[0004] However, as people age, they tend to experience more dry skin, and the demand for skin moisturizers increases due to factors such as indoor heating and air pollution. Therefore, polyols and sugars are commonly used in conjunction with cosmetics as skin moisturizers. However, they have drawbacks such as causing a sticky feeling on the skin and not providing sufficient moisturizing effects.

[0005] Furthermore, in the high humidity of summer, skin absorbs moisture from the outside, leading to a sticky feeling, while in winter, low external humidity causes the skin to lose moisture and become dry. Human skin is very sensitive to relative humidity, and generally feels comfortable at a relative humidity of 40-70%. Relative humidity is expressed as a percentage (%) within 1m³. 3 The ratio of the amount of water vapor in the air to the amount of saturated water vapor at the same temperature. In South Korea, summers are very humid due to high humidity, while winters are very dry due to low relative humidity. Seasonal changes and humidity variations also significantly affect human skin. High relative humidity in the summer, caused by high temperature and humidity, increases discomfort, while dry winters, due to low relative humidity, can cause lips or skin to crack due to dryness, and sometimes even difficulty breathing. This is because when relative humidity is high, skin absorbs moisture from the air and becomes sticky; conversely, when relative humidity is low, skin moisture evaporates and becomes dry. In particular, when relative humidity is low, dry skin is often accompanied by tightness and itching.

[0006] Various cosmetic compositions utilize a variety of moisturizers, such as oils, sugars, and polyols, to combat dry skin. For example, by using skin-derived components like ceramides, cholesterol, and fatty acids, the skin barrier function is strengthened, thereby inhibiting moisture evaporation. Alternatively, sugars and polyols, which have a strong water-binding capacity on the skin surface, are used to enhance the skin's ability to retain moisture, thus improving moisturizing effects. However, this effect cannot be maintained for long periods in the low relative humidity of winter, and in the high relative humidity of summer, it can cause discomfort. It is difficult to formulate suitable cosmetic compositions based on changes in relative humidity.

[0007] To address this issue, existing technologies primarily attempt to resolve the problems described above through chemical ingredients. However, cosmetics using chemical ingredients may cause side effects and are ecologically unsustainable. Therefore, research is underway on cosmetic ingredients that combine various natural plant materials.

[0008] Existing technical documents

[0009] Patent documents

[0010] Patent Document 0001: Korean Patent Publication No. 10-2348707 (Publication Date: January 6, 2022), "A cosmetic or pharmaceutical composition comprising docetaxel or a pharmaceutically permissible salt thereof for the purpose of improving skin elasticity, wrinkle improvement, moisturizing, or anti-inflammation." Summary of the Invention

[0011] Technical problems to be solved

[0012] To address the problems described above, the present invention aims to provide a cosmetic composition containing a mixture of cotton, bitter melon, hibiscus, and hollyhock extracts as active ingredients to achieve a moisturizing effect, and a method for preparing the same.

[0013] Technical solutions to the problem

[0014] To achieve the objectives described above, the present invention provides a cosmetic composition comprising a mixed extract of cotton, bitter melon, hibiscus and hollyhock as an active ingredient, characterized in that it comprises a mixed extract obtained by extracting a mixture of cotton, bitter melon, hibiscus and hollyhock.

[0015] Furthermore, the present invention is characterized in that the above mixture is mixed with cotton, bitter melon, hibiscus and hollyhock in a weight ratio of 0.1-2.5:0.1-2.5:0.1-2.5:0.1-2.5 respectively.

[0016] Furthermore, the present invention is characterized in that the above-mentioned mixed extract is extracted using any one of the following extraction solvents selected from the group consisting of purified water, anhydrous or aqueous lower alcohols having a carbon number of 1 to 4, acetone, ethyl acetate, butyl acetate, and 1,3-butanediol.

[0017] Furthermore, the present invention is characterized in that, after cooling the above-mentioned mixed extract to room temperature, it is filtered with a filter cloth and concentrated under reduced pressure.

[0018] Furthermore, the present invention provides a method for preparing a cosmetic composition comprising a mixed extract of cotton, bitter melon, hibiscus, and hollyhock as an active ingredient, comprising: a mixture preparation step S10, wherein cotton, bitter melon, hibiscus, and hollyhock are mixed to prepare a mixture; an extraction step S20, wherein the mixture prepared in the mixture preparation step S10 is mixed with any one of the following extraction solvents selected from the group consisting of purified water, anhydrous or aqueous lower alcohols having a carbon number of 1 to 4, acetone, ethyl acetate, butyl acetate, and 1,3-butanediol to extract the mixed extract; and a filtration and concentration step S30, wherein the mixed extract in the extraction step S20 is cooled, filtered, and concentrated under reduced pressure.

[0019] Furthermore, in the extraction step S20 described above, the mixture prepared in the mixture preparation step S10 and the extraction solvent are mixed at a weight ratio of 0.1 to 2.0: 10.0 to 30.0, and extracted at a temperature of 80 to 100°C for 2 to 6 hours.

[0020] Furthermore, in the above-mentioned filtration and concentration step S30, the mixed extract from the above-mentioned extraction step S20 is cooled at room temperature, and then filtered with a 200-600 mesh filter cloth and concentrated under reduced pressure.

[0021] Beneficial effects of the invention

[0022] The cosmetic composition prepared according to the method of the present invention, which contains a mixed extract of cotton, bitter melon, hibiscus and hollyhock as an active ingredient, has a moisturizing effect because it contains a mixed extract of cotton, bitter melon, hibiscus and hollyhock as an active ingredient. Attached Figure Description

[0023] Figure 1 The flowchart illustrates the preparation process of a cosmetic composition comprising a mixed extract of cotton, bitter melon, hibiscus, and hollyhock as an active ingredient, according to the present invention.

[0024] Figure 2 The bar chart shows the collagen synthesis-promoting effects of Preparation Examples 1 to 5.

[0025] Figure 3 This is a bar chart showing the evaluation of moisturizing gene expression in Preparation Examples 1 to 5.

[0026] Figure 4 This is a bar chart showing the induction effect of keratinocyte formation in the keratinocytes prepared in Examples 1 to 5.

[0027] Figure 5 The bar chart shows the skin moisturizing improvement effect of Example 1 and Comparative Examples 1 to 4.

[0028] Figure 6 This is a bar chart showing the transdermal water loss for Example 1 and Comparative Examples 1 to 4.

[0029] Explanation of reference numerals in the attached figures

[0030] S10: Mixture preparation steps

[0031] S20: Extraction Steps

[0032] S30: Filtration and Concentration Steps Detailed Implementation

[0033] The following detailed description of the invention describes embodiments in which the invention can be practiced, with reference to the accompanying drawings shown as examples of corresponding embodiments. These embodiments will be described in detail below to enable those skilled in the art to fully implement the invention. It should be understood that while the various embodiments of the invention are different from each other, they are not mutually exclusive. For example, a particular shape, structure, and characteristic described herein with respect to one embodiment may be embodied in other embodiments without departing from the spirit and scope of the invention. Furthermore, it should be understood that the position or arrangement of individual structural elements within the various described embodiments may be modified without departing from the spirit and scope of the invention.

[0034] Therefore, the detailed description that follows is not intended to be limiting; if described in detail, the scope of the invention is limited only to all the scope equivalent to that claimed in the claims and the appended claims. Similar reference numerals in the drawings denote the same or similar functions on multiple levels.

[0035] Considering the function in this invention, the terminology used herein is selected as widely used and common as possible. However, this may change due to the intentions or precedents of those skilled in the art, the emergence of new technologies, etc. Furthermore, in certain cases, terms may be arbitrarily chosen by the applicant; in such cases, their meanings will be detailed in the description of the corresponding invention. Therefore, the terminology used in this invention should be defined based on its meaning and the overall content of the invention, rather than the name of the term itself.

[0036] In this invention, when a part "includes" a structural element, unless otherwise specifically stated to the contrary, it means that other structural elements may also be included, rather than excluding other structural elements.

[0037] The following describes in detail the cosmetic composition of the present invention, which contains a mixed extract of cotton, bitter melon, hibiscus and hollyhock as an active ingredient, and its preparation method.

[0038] The cosmetic composition of the present invention is characterized in that it contains a mixture of extracts of cotton, bitter melon, hibiscus and hollyhock as active ingredients.

[0039] The aforementioned cotton (Gossypium hirsutum) is an annual plant belonging to the Malvaceae family of the Malvaceae order in the class Dicotyledonous plants. It is also known as cotton fiber (cotton cotton) or grass cotton. Although native to tropical regions, it is also widely cultivated as a fiber crop in temperate regions. It is usually an annual plant, but some varieties are low shrubs. In temperate zones, it can grow to about 90cm in height, and in tropical zones, it can reach 2m. Its roots extend straight, and its stems grow straight with branching branches.

[0040] The aforementioned bitter gourd (Momordica charantia Linnaeus) is a climbing annual plant belonging to the Cucurbitaceae family in the Cucurbitales order of dicotyledons. Its leaves are alternate and have long petioles. The leaves are divided into 5-7 lobes and are serrated. The fruit is oval and covered with tubercles-like protrusions. It is monoecious, with solitary yellow flowers borne in the leaf axils. This bitter gourd is a climbing plant and thrives in hot and humid climates.

[0041] The aforementioned hibiscus (Hibiscus syriacus (Althaea frutex)) is a deciduous shrub belonging to the Malvaceae family, also known as the hibiscus flower. It is cold-hardy, reaching a height of 2–4 meters, sometimes almost growing into a tree. The plant is entirely hairless, with numerous branches, and is gray in color. Leaves are late-budding and alternate, with short petioles. The leaf blades are rhomboid or ovate, 4–6 cm long and 2.5–5 cm wide, shallowly lobed into three lobes with irregularly serrated edges. The upper surface of the leaves is hairless, but the underside is hairy. Flowers are solitary in the axils of newly grown leaves, bell-shaped, with short pedicels.

[0042] The aforementioned hollyhock (Althaea rosea) is a Malvaceae family flower with a long history, growing throughout Korea. If sown in spring or summer, it will only produce dense foliage that year, with stems and flowers emerging the following year. The flowers come in various colors and are either single or double-petaled. The stems, flowers, leaves, and roots can be used as traditional Chinese medicine.

[0043] Preferably, the composition of the present invention comprises a mixed extract obtained by extracting a mixture of cotton, bitter melon, hibiscus and hollyhock in a weight ratio of 0.1-2.5:0.1-2.5:0.1-2.5:0.1-2.5 by an extraction solvent, and more specifically, preferably in a weight ratio of 1:1:1:1.

[0044] In this case, it is suitable to extract the mixture of cotton, bitter melon, hibiscus and hollyhock using any one of the following extraction solvents selected from the group consisting of purified water, anhydrous or hydrous lower alcohols with a carbon number of 1 to 4, acetone, ethyl acetate, butyl acetate and 1,3-butanediol, and most specifically, purified water is preferred.

[0045] On the other hand, in the composition of the present invention, extraction can be performed by mixing a mixture of cotton, bitter melon, hibiscus, and hollyhock with an extraction solvent in a weight ratio of 0.1 to 2.0: 10.0 to 30.0. More specifically, extraction is most preferably performed by mixing a mixture of cotton, bitter melon, hibiscus, and hollyhock with an extraction solvent in a weight ratio of 1.0: 20.0.

[0046] Furthermore, for the composition of the present invention, when extracting the mixture of cotton, bitter melon, hibiscus, and hollyhock with the extraction solvent, it is preferable to extract at a temperature of 80–100°C for 2–6 hours. More specifically, most preferably, the mixture of cotton, bitter melon, hibiscus, and hollyhock with the extraction solvent can be mixed and extracted at a temperature of 90°C for 4 hours.

[0047] On the other hand, preferably, the composition of the present invention is used to extract a mixture of cotton, bitter melon, hibiscus and hollyhock with an extraction solvent, and after cooling to room temperature, it is filtered with a 200-600 mesh filter cloth and concentrated under reduced pressure.

[0048] In summary, the composition of the present invention can be obtained as follows: a mixture obtained by mixing cotton, bitter melon, hibiscus and hollyhock in a weight ratio of 0.1-2.0:10.0-30.0 and cotton, bitter melon, hibiscus and hollyhock in a weight ratio of 0.1-2.5:0.1-2.5:0.1-2.5:0.1-2.5 with any one of the following extraction solvents selected from the group consisting of purified water, anhydrous or aqueous lower alcohols with a carbon number of 1 to 4, acetone, ethyl acetate, butyl acetate and 1,3-butanediol, and extracting at a temperature of 80-100°C for 2-6 hours, cooling to room temperature, filtering with a 200-600 mesh filter cloth and concentrating under reduced pressure.

[0049] Ideally, a mixture of cotton, bitter melon, hibiscus and hollyhock in a weight ratio of 1:20 and purified water is mixed and extracted at 90°C for 4 hours. After cooling to room temperature, the mixture is filtered through a 400-mesh filter cloth and concentrated under reduced pressure.

[0050] In addition to the compositions of the present invention, the present invention includes ingredients commonly used in cosmetic compositions, such as general excipients and carriers including antioxidants, stabilizers, solubilizers, vitamins, pigments, colorants and fragrances.

[0051] The cosmetic compositions of the present invention can be formulated into all dosage forms commonly prepared in the art, such as solutions, suspensions, lotions, creams, gels, balms, liquids, powders, soaps, surfactant-containing cleansers, oils, powder foundations, emulsion foundations, wax foundations, and sprays, but are not limited thereto. More specifically, they can be formulated into softening lotions, nourishing lotions, nourishing creams, massage creams, serums, masks, eye creams, cleansing balms, cleansing foams, makeup removers, masks, sprays, or powders.

[0052] When the dosage form of the present invention is an ointment, cream, or gel, one or more of the following can be used as a carrier component: animal oil, vegetable oil, wax, paraffin, starch, tragacanth gum, cellulose derivatives, polyethylene glycol, silicone, bentonite, silica, talc, or zinc oxide.

[0053] When the dosage form of the present invention is powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, and polyamide powder can be used as carrier components. Especially in the case of spray, accelerators such as chlorofluorocarbons, propane / butane, or dimethyl ether can be additionally included.

[0054] When the dosage form of the present invention is a solution or emulsion, a solvent, solubilizer, or emulsifier is used as a carrier component. For example, one or more of the following can be used: water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, butylene glycol, 1,3-butanediol oil, polyoxyethylene hydrogenated castor oil, glycerol, glycerin, aliphatic esters, phenoxyethanol, triethanolamine, polyethylene glycol, beeswax, polysorbate 60, sorbitan sesquioleate, paraffin wax, dehydrated sorbitan stearate, lipophilic monostearate glycerol, stearic acid, glyceryl stearate / polyethylene glycol 400 stearate, carboxyl polymers, sitosterol, polyglycerol 2-oleate, ceramide, cholesterol, steareth-4, dihexadecanophosphate, macadamia nut oil, carboxyl vinyl polymers, xanthan gum, or sorbitan fatty acid esters.

[0055] When the dosage form of the present invention is a suspension, one or more of the following can be used as the carrier component: liquid diluents such as water, ethanol, glycerin, butylene glycol or propylene glycol; suspensions such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitan ester and polyoxyethylene dehydrated sorbitan ester; microcrystalline cellulose, hydroxyethyl cellulose, sodium hyaluronate, phenoxyethanol, aluminum hydroxide, bentonite, stearic acid, cetyl alcohol, glyceryl monostearate, polyoxyethylene dehydrated sorbitan monostearate, dehydrated sorbitan sesquioleate, glyceryl monostearate / glyceryl stearate / polyoxyethylene stearate, wax, paraffin, squalane, caprylic / capric triglyceride, carboxyvinyl polymer, triethanolamine, agar or tragacanth.

[0056] When the dosage form of the present invention is a surfactant-containing detergent, one or more of the following can be used as the carrier component: fatty alcohol sulfate, fatty alcohol ether sulfate, sulfosuccinate monoester, hydroxyethyl sulfonate, imidazoline derivative, methyl taurate, sarcosinate, fatty acid amide ether sulfate, cocamidopropyl betaine, fatty alcohol, fatty acid glycerides, fatty acid diethanolamide, vegetable oil, lanolin derivative, or ethoxylated glycerol fatty acid ester.

[0057] Furthermore, the efficacy of the cosmetic composition according to an embodiment of the present invention can be appropriately adjusted according to an individual's skin condition and external environmental conditions.

[0058] The following will describe in detail the preparation method of the cosmetic composition of the present invention, which contains a mixed extract of cotton, bitter melon, hibiscus and hollyhock as active ingredients.

[0059] The method for preparing a cosmetic composition comprising a mixed extract of cotton, bitter melon, hibiscus, and hollyhock as an active ingredient according to the present invention includes: a mixture preparation step S10, in which cotton, bitter melon, hibiscus, and hollyhock are mixed to prepare a mixture; an extraction step S20, in which the mixture prepared in the mixture preparation step S10 is mixed with any one of the following extraction solvents selected from the group consisting of purified water, anhydrous or aqueous lower alcohols having a carbon number of 1 to 4, acetone, ethyl acetate, butyl acetate, and 1,3-butanediol to extract the mixed extract; and a filtration and concentration step S30, in which the mixed extract in the extraction step S20 is cooled, filtered, and concentrated under reduced pressure.

[0060] First, perform the mixture preparation step S10.

[0061] In step S10 of the above mixture preparation, cotton, bitter melon, hibiscus and hollyhock are mixed to prepare the mixture.

[0062] Preferably, in the above mixture preparation step S10, the mixture is mixed with cotton, bitter melon, hibiscus and hollyhock in a weight ratio of 0.1-2.5:0.1-2.5:0.1-2.5:0.1-2.5.

[0063] More specifically, preferably, the mixture in the above-mentioned mixture preparation step S10 is mixed with cotton, bitter melon, hibiscus and hollyhock in a weight ratio of 1:1:1:1.

[0064] Then, perform extraction step S20.

[0065] In the extraction step S20 above, the mixture prepared in the mixture preparation step S10 above is mixed with any one of the following extraction solvents selected from the group consisting of pure water, anhydrous or aqueous lower alcohols with 1 to 4 carbon atoms, acetone, ethyl acetate, butyl acetate and 1,3-butanediol and extracted to prepare a mixed extract.

[0066] In the extraction step S20 above, the mixture prepared in the mixture preparation step S10 above is mixed with any one of the following extraction solvents selected from the group consisting of pure water, anhydrous or aqueous lower alcohols with 1 to 4 carbon atoms, acetone, ethyl acetate, butyl acetate and 1,3-butanediol at a weight ratio of 0.1 to 2.0:10.0 to 30.0, and extracted at a temperature of 80 to 100°C for 2 to 6 hours.

[0067] More specifically, preferably, in the extraction step S20 above, the mixture prepared in the mixture preparation step S10 above is mixed with purified water at a weight ratio of 1:20, and extracted at a temperature of 90°C for 4 hours.

[0068] Then, perform the filtration and concentration step S30.

[0069] In the above-mentioned filtration and concentration step S30, the mixed extract from the above-mentioned extraction step S20 is cooled, filtered, and concentrated under reduced pressure.

[0070] In the above-mentioned filtration and concentration step S30, the mixed extract from the above-mentioned extraction step S20 is cooled at room temperature, and then filtered with a 200-600 mesh filter cloth and concentrated under reduced pressure.

[0071] More specifically, preferably, in the above-described filtration and concentration step S30, the mixed extract from the above-described extraction step S20 is cooled at room temperature, then filtered with a 400-mesh filter cloth and concentrated under reduced pressure.

[0072] The effects of the cosmetic compositions of the present invention will be described in detail below through the following preparation examples, embodiments, comparative examples and experimental examples.

[0073] Preparation Example 1. Preparation of the mixed extract of the present invention

[0074] The mixture of cotton, bitter melon, hibiscus and hollyhock in a weight ratio of 1:20 and pure water was mixed and extracted at 90°C for 4 hours. After cooling to room temperature, it was filtered through a 400-mesh filter cloth and concentrated under reduced pressure.

[0075] Preparation Example 2. Preparation of extracts from cotton alone

[0076] Cotton and purified water were mixed at a weight ratio of 1:20 and extracted at 90°C for 4 hours. After cooling to room temperature, the mixture was filtered through a 400-mesh filter cloth and concentrated under reduced pressure.

[0077] Preparation Example 3. Preparation of an extract from bitter melon alone

[0078] Bitter melon and purified water were mixed at a weight ratio of 1:20 and extracted at 90°C for 4 hours. After cooling to room temperature, the mixture was filtered through a 400-mesh filter cloth and concentrated under reduced pressure.

[0079] Preparation Example 4. Preparation of an extract from hibiscus flowers alone

[0080] Hibiscus flowers and purified water were mixed at a weight ratio of 1:20 and extracted at 90°C for 4 hours. After cooling to room temperature, the mixture was filtered through a 400-mesh filter cloth and concentrated under reduced pressure.

[0081] Preparation Example 5. Preparation of an extract from marshmallow alone

[0082] The marshmallow and purified water were mixed at a weight ratio of 1:20 and extracted at 90°C for 4 hours. After cooling to room temperature, the mixture was filtered through a 400-mesh filter cloth and concentrated under reduced pressure.

[0083] Example 1 and Comparative Examples 1 to 4. Preparation of dosage forms using Preparation Examples 1 to 5.

[0084] As shown in Table 1 below, Example 1 and Comparative Examples 1 to 4 were prepared by adapting the dosage forms of the above-mentioned Preparation Examples 1 to 5.

[0085] Table 1

[0086]

[0087] Experimental Example 1. Confirming the effect of promoting collagen biosynthesis - Collagen synthesis

[0088] The collagen production promoting effects of the above-mentioned preparation examples 1 to 5 were measured.

[0089] 1) Experimental methods

[0090] Human dermal fibroblasts (Lonza, Switzerland) were subcultured in DMEM containing 10% fetal bovine serum (FBS) and 1% penicillin-streptomycin, and then seeded into 96-well cell culture plates. The cells were cultured at 37°C and 5% CO2 for 24 hours. Afterward, the medium was replaced with serum-free medium, and diluted samples were added to the cells. The cells were cultured for another 24 hours, and the supernatant was collected. Collagen synthesis was measured using a procollagen type I C-peptide PIP EIAkit (Takara, Japan). 20 μL of supernatant from each well was mixed with 100 μL of antibody-POD conjugate solution dissolved at room temperature and reacted for 3 hours. After washing three times with 500 μL of phosphate-buffered saline, 100 μL of the colorimetric reagent was treated each time. After 15 minutes of reaction, an equal volume of 1N sulfuric acid (Daejung, South Korea) was added and the reaction proceeded. The result was then measured using a microplate reader (VersaMax). TM The absorbance was measured at 450 nm in the United States.

[0091] 2) Experimental Results

[0092] The collagen production promotion rate is calculated using the following mathematical formula 2, as shown in Table 2 below.

[0093] Mathematical formula 2

[0094]

[0095] Table 2

[0096]

[0097] The experimental results are shown in Table 2 and Figure 2 As shown, it can be seen that, compared with Preparation Examples 2 to 5, Preparation Example 1, which contains the mixed extract of the present invention, has the best collagen synthesis promoting effect at different treatment concentrations.

[0098] Experimental Example 2. Evaluation of Moisturizing Gene Expression - Gene Expression Assay

[0099] To confirm the promoting effect of the hyaluronic acid TGM1 gene on messenger ribonucleic acid (mRNA) synthesis in Preparation Examples 1 to 5, real-time polymerase chain reaction (real-time PCR) experiments were performed.

[0100] In a 6-well plate at 2.5 x 10 5 After treating the human keratinocyte cell line HaCaT cells with cells / wells, they were cultured for 24 hours at 37°C in DMEM, 10% fetal bovine serum (FBS), and 1% penicillin-streptomycin medium. After 24 hours, the medium used for the previous culture was removed, and Preparation Examples 1 to 5 were treated with concentrations of 0.1%, 0.5%, 1%, 3%, and 5% respectively in the medium, and reacted for 18 hours at 37°C in 5% carbon dioxide (CO2).

[0101] Cells were recovered 18 hours later, and ribonucleic acid (RNA) was extracted using an RNA extraction kit (intron). After determining the concentration and purity of the RNA, complementary deoxyribonucleic acid (cDNA) was synthesized using 1 μg of RNA using a complementary deoxyribonucleic acid (cDNA) synthesis kit. Real-time polymerase chain reaction (real-time PCR) was then performed using glyceraldehyde-3-phosphate dehydrogenase primer (GAPDH primer).

[0102] Table 3 below shows the expression levels of messenger ribonucleic acid (mRNA) of the TGM1 gene at different concentrations in Preparation Examples 1 to 5. It can be confirmed that the expression level of TGM1 messenger ribonucleic acid (mRNA) in the experimental group treated with Preparation Example 1 increased compared with Preparation Examples 2 to 5.

[0103] Table 3

[0104]

[0105] Experimental Example 3. Confirming the keratinocyte formation induction effect - Cornified Envelope assay

[0106] The keratinocyte formation induction effect in the keratinocytes of Preparation Examples 1 to 5 was confirmed.

[0107] 1) Experimental methods

[0108] Human normal keratinocytes were subcultured in a medium containing growth factors. They were then seeded onto plates and cultured at 37°C with 5% carbon dioxide (CO2) until 70-80% confluence. The differentiation capacity of epidermal cells was then tested by treating samples from Preparations 1 to 5 with different concentrations. In this case, the 1.20 mM CA2+ treatment group was used as a positive control. After culturing at 37°C for 48 hours, the cells were washed with phosphate-buffered saline (PBS) and recovered. The cells were then boiled at 85°C for 15 minutes to elute them. A portion was used as a baseline for protein quantification, and the remaining fraction was centrifuged (1200 rpm, 30 minutes). The separated precipitate was resuspended in PBS, and the absorbance was measured at 340 nm. The 0.03 mM CA2+ treatment was then... 2+ The group was used as a 100% negative control group, and its relative degree of epidermal differentiation was compared.

[0109] 2) Experimental Results

[0110] The effects of keratinocytes on keratinocyte formation induction are shown in Table 4 below.

[0111] Table 4

[0112]

[0113] The experimental results are shown in Table 4 and Figure 4 As shown, it can be seen that, compared with Preparation Examples 2 to 5, Preparation Example 1, which contains the mixed extract of the present invention, has the best keratinocyte formation induction effect.

[0114] Experiment Example 4. Confirming the skin moisturizing effect

[0115] 1) Experimental methods

[0116] The cosmetic compositions prepared in Example 1 and Comparative Examples 1 to 4 were applied twice a day for four consecutive weeks to both sides and the front of the face of 30 test subjects (men and women aged 20-45 years). The improvement in skin hydration was measured before application, two weeks after application, and four weeks after application using a skin moisture meter (Corneometer CM 820, Courage+Khazaka, Germany). The unit was any unit (Arbitrary Unit, AU) provided by the instrument. The higher the measured value, the higher the moisture content of the skin surface.

[0117] 2) Experimental Results

[0118] The experimental results on skin hydration improvement are shown in Table 5.

[0119] Table 5

[0120]

[0121] The experimental results are shown in Table 5 and Figure 5 As shown, it can be seen that, compared with Comparative Examples 1 to 4, Example 1, which contains the mixed extract of the present invention, has the best skin moisturizing improvement effect.

[0122] Experimental Example 5. Confirmation of the rate of inhibition of transdermal water loss

[0123] 1) Experimental methods

[0124] The cosmetic compositions prepared in Example 1 and Comparative Examples 1 to 4 were used to designate the upper arm area of ​​30 test subjects (men and women aged 20-45 years) as the test site, and the area was marked with a horizontal and vertical dimension of 2×2cm. 2 Transdermal water loss was measured after applying the cosmetic composition to the test sites. The sites were thoroughly massaged and transdermal water loss was measured using a skin moisture loss tester (TEWAMETER TM210, C+K eletronic GmhH., Germany). The percentage increase in moisture content was measured at 30 minutes, 1 day, 4 days, and 8 days after application, using the initial moisture content at 0 hours as a baseline, to evaluate the short-term moisturizing effect. During measurement, the probe was held vertically in contact with the skin for 60 seconds, and the inhibition rate of transdermal water loss was measured three times consecutively. Measurements were conducted under identical conditions in a temperature and humidity controlled chamber; higher values ​​indicate less moisture loss.

[0125] Table 6

[0126]

[0127] The experimental results are shown in Table 6 and Figure 6 As shown, it can be seen that, compared with Comparative Examples 1 to 4, Example 1, which contains the mixed extract of the present invention, has the best transdermal water loss inhibition rate.

[0128] Dosage Form Example 1. Preparation of a toner dosage form

[0129] Examples of toner formulations using the mixed extracts of the present invention are shown in Table 7 below.

[0130] Table 7

[0131]

[0132] Dosage Form Example 2. Preparation of Emulsion Dosage Form

[0133] Examples of emulsion formulations using the mixed extracts of the present invention are shown in Table 8 below.

[0134] Table 8

[0135]

[0136]

[0137] Dosage Form Example 3. Preparation of Cream Dosage Form

[0138] Examples of cream formulations using the mixed extracts of the present invention are shown in Table 9 below.

[0139] Table 9

[0140]

[0141]

[0142] Although the invention has been described in conjunction with the accompanying drawings, this is merely one embodiment of various implementations that embody the spirit of the invention, intended to facilitate practice by those skilled in the art. Clearly, the invention is not limited to the embodiments described above. Therefore, the scope of protection of the invention should be interpreted according to the appended claims. All technical ideas within the same scope, formed through modifications, substitutions, or replacements, without departing from the spirit of the invention, are entitled to the rights of the invention. Furthermore, it should be understood that, for the purpose of more clearly illustrating the structure, some structures in the drawings are exaggerated or reduced compared to their actual dimensions.

Claims

1. A cosmetic composition comprising a mixture of extracts of cotton, bitter melon, hibiscus, and hollyhock as active ingredients, characterized in that, A mixed extract comprising a mixture of cotton, bitter melon, hibiscus and hollyhock, wherein the mixture is mixed with cotton, bitter melon, hibiscus and hollyhock in a weight ratio of 0.1-2.5:0.1-2.5:0.1-2.5:0.1-2.5, and the mixed extract is extracted with any one of the following extraction solvents selected from the group consisting of purified water and anhydrous or aqueous lower alcohols having a carbon number of 1 to 4.

2. The cosmetic composition according to claim 1, comprising a mixed extract of cotton, bitter melon, hibiscus, and hollyhock as an active ingredient, is characterized in that... After cooling the above mixed extract to room temperature, it was filtered with a filter cloth and concentrated under reduced pressure.

3. A method for preparing a cosmetic composition comprising a mixed extract of cotton, bitter melon, hibiscus, and hollyhock as active ingredients, characterized in that, include: The mixture preparation step (S10) involves mixing cotton, bitter melon, hibiscus, and hollyhock to prepare a mixture, wherein the mixture is prepared by mixing cotton, bitter melon, hibiscus, and hollyhock in a weight ratio of 0.1-2.5:0.1-2.5:0.1-2.5:0.1-2.

5. In the extraction step (S20), the mixture prepared in the above mixture preparation step (S10) is mixed with any one of the extraction solvents selected from the group consisting of purified water and anhydrous or aqueous lower alcohols having a carbon number of 1 to 4 to extract a mixed extract. as well as The filtration and concentration step (S30) involves cooling, filtering, and concentrating the mixed extract from the extraction step (S20) under reduced pressure.

4. The method for preparing the cosmetic composition comprising a mixed extract of cotton, bitter melon, hibiscus, and hollyhock as an active ingredient according to claim 3, characterized in that, In the above extraction step (S20), the mixture prepared in the above mixture preparation step (S10) and the extraction solvent are mixed at a weight ratio of 0.1 to 2.0: 10.0 to 30.0, and extracted at a temperature of 80 to 100°C for 2 to 6 hours.

5. The method for preparing a cosmetic composition comprising a mixed extract of cotton, bitter melon, hibiscus, and hollyhock as an active ingredient, as described in claim 3, is characterized in that... In the above-mentioned filtration and concentration step (S30), the mixed extract from the above-mentioned extraction step (S20) is cooled at room temperature, and then filtered with a 200-600 mesh filter cloth and concentrated under reduced pressure.

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