Preparation of a core-shell structured nanomaterial and its application in the detection of traditional Chinese medicine.

Abstract

This invention relates to the preparation of core-shell structured nanomaterials and their application in the detection of traditional Chinese medicine, belonging to the field of traditional Chinese medicine detection technology. The method includes the following steps: Purifying gold nanoparticles; taking 10 mL of colloidal gold and centrifuging at 8000 rpm and 4°C for 10 min; discarding the supernatant; resuspending the precipitate in 8 mL of deionized water; adding dilute acid dropwise to the resuspended solution and stirring until the pH reaches 4; then adding 2 mL of freshly prepared 0.1-0.5 mol / L ascorbic acid solution; followed by adding 0.1-2 mmol / L AgNO3 solution dropwise while stirring, approximately one drop every 20 seconds. The solution color gradually changes from purple to orange-yellow. Stop adding the solution and stir for approximately 30 min until stable; centrifuge at 6500 rpm and 4°C for 10 min; discarding the supernatant; resuspending the precipitate in 6 mL of water; this solution is the core-shell structured nanomaterial. The detection process of this invention only takes 15 minutes and the detection limit can reach 0.05 mg / kg. It can achieve accurate identification at the molecular level and greatly improve the reliability of the detection results. The operation steps are simple and fast, and do not require professional operators or large instruments and equipment.

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