A composition containing citronella oil, a preparation thereof, and a method of preparing and using the same

Abstract

The application belongs to the technical field of oral care, and provides a composition containing citronella essential oil, a preparation, a preparation method and application thereof; the composition comprises: active ingredient A and active ingredient B; the active ingredient A is citronella essential oil; the citronella essential oil is at least one of Cymbopogon narbus, Cymbopogon citratus and Cymbopogon khasans; and the active ingredient B is cassia bark essential oil. The composition of the application has obvious bacteriostatic activity on Streptococcus mutans under the synergistic action of citronella essential oil and cassia bark essential oil, has a short bactericidal time, has potential application in the preparation of drugs for treating dental caries caused by Streptococcus mutans, has simple composition, does not contain other chemical components, has no toxicity and irritation, and has wide application in the preparation of oral care products.

Description

Technical Field

[0001] This invention belongs to the field of oral care technology, and relates to a composition containing lemongrass essential oil, a preparation thereof, its preparation method and application. Background Technology

[0002] Dental caries is a chronic infectious disease of the oral cavity, mainly manifested as chronic progressive lesions of the hard tissues of the teeth, characterized by high incidence and wide distribution. Dental caries is a gradual process related to the activity of specific types of bacteria, and *Streptococcus mutans* (S. mutans) is one of the main pathogens of dental caries, a major cariogenic bacterium in dental plaque biofilm. *Streptococcus mutans* aggregates and colonizes the tooth surface and produces acid, thereby eroding the enamel, causing demineralization, and subsequently invading the dentin, leading to caries. Therefore, reducing the number of cariogenic bacteria, decreasing acid synthesis, and preventing plaque biofilm formation are the most effective strategies for preventing and controlling dental caries.

[0003] Currently, antibiotic-containing drugs are commonly used to treat dental caries. Chinese invention patent application CN115844765A discloses an antibacterial and anti-caries oral care composition and its preparation method. This antibacterial and anti-caries oral care composition, by weight, includes 0.05-0.2 parts of fluoride, 2-3 parts of zinc oxide, 0.5-5 parts of hyaluronic acid composition, and 1-2 parts of peppermint essential oil. The composition can be used as an oral antibacterial agent and anti-caries agent, effectively inhibiting the activity of bacteria and fungi and their acid-producing ability, and has a strong inhibitory effect on glucosyltransferase activity. Chinese invention patent CN114533669B discloses an oral care composition, its preparation method, and its application. The composition includes drug-loaded micelles, which consist of polymer micelles and an enamel repair drug physically encapsulated within the polymer micelles. The oral care composition adheres to the tooth surface via salivary protein peptides. In the acidic oral environment caused by tooth decay, the composition breaks the borate ester bond between tannic acid and the water-soluble polymer, releasing tannic acid and sodium fluoride. This enables intelligent and on-demand drug release, thereby achieving the purpose of preventing tooth decay and repairing tooth defects.

[0004] However, existing chemical drugs often cause side effects, such as enamel discoloration and altered taste. Furthermore, long-term use can easily lead to drug resistance. Using varnishes, mouthwashes, or incorporating antibacterial drugs into filling materials to improve the antibacterial properties of the restorative interface also suffers from drawbacks such as short-lasting drug release and lack of specificity. Therefore, finding and developing safe, effective, and healthy plant-based drugs for treating dental caries is particularly important.

[0005] Plant essential oils possess significant advantages in antibacterial and anti-biofilm properties, while also exhibiting high safety and no side effects, meeting the requirements for oral care products and making them an ideal alternative to chemical antibiotics in the oral health industry. The main chemical components of cinnamon bark are terpenes and aldehydes, while the volatile substances obtained from cinnamon leaves are alcohols, alkenes, and their oxides. Lemongrass is commonly used in traditional medicine to treat nervous and gastrointestinal disorders; it is an antispasmodic, analgesic, anti-inflammatory, antipyretic, diuretic, and sedative agent. Chinese invention patent application CN105943903A discloses a drug for treating toothache caused by dental caries, comprising cinnamon oil, lemongrass oil, camphor oil, clove oil, wintergreen oil, peony bark, angelica dahurica, asarum, peppermint, gypsum, iodine, potassium iodide, and ethanol—a combination of traditional Chinese and Western medicine. Its active ingredients can be continuously released, but it still requires the addition of chemical components such as iodine and potassium iodide.

[0006] Different essential oils contain varying amounts of compounds, and these compounds exhibit different antibacterial activities, leading to significant differences in antibacterial effects. Blended functional essential oils, due to the synergistic interactions between their compounds, possess multi-target antibacterial effects against target microorganisms; these compounds can inhibit a variety of microorganisms. Generally, higher concentrations of essential oils are required to achieve the same effects in products as in vitro. Achieving effective antibacterial activity at sufficiently low concentrations through the blending of different essential oils, without adversely affecting the sensory acceptability of food, is a pressing issue. Currently, although some oral care products contain essential oils, research on the antibacterial activity of these blends of different essential oils against Streptococcus mutans is scarce. Summary of the Invention

[0007] This invention addresses the problems existing in the prior art by providing a composition, formulation, preparation method, and application containing lemongrass essential oil. Early in vitro studies of essential oils have shown that they possess good antibacterial properties; however, when applied to product matrices, the amount required to significantly inhibit bacterial growth is often higher than sensorily acceptable levels. These high concentrations may affect certain sensory characteristics and stability of the product. Adding a suitable proportion of the composition to oral care products may be a way to achieve a balance between sensory acceptability and antibacterial efficacy. Based on this, this invention enhances the antibacterial ability of a single essential oil through the synergistic effect of blending different essential oils, expands its antibacterial spectrum, and reduces the effective dosage of essential oil and the cost of the product.

[0008] To achieve the above objectives, the technical solution adopted by the present invention is as follows:

[0009] A composition containing lemongrass essential oil, the composition comprising: active ingredient A and active ingredient B; said active ingredient A is lemongrass essential oil; said active ingredient B is cinnamon bark essential oil (Cinnamomum burmani).

[0010] Preferably, the citronella essential oil is at least one of citronella essential oil (Cymbopogon narbus), lemon citronella essential oil (Cymbopogon citratus), and rose citronella essential oil (Cymbopogon khasans).

[0011] Preferably, the composition comprises, by volume ratio: active component A: active component B = 1-4: 1-5.

[0012] More preferably, the composition comprises, by volume ratio: lemongrass essential oil: cinnamon bark essential oil = 1-4: 1-4, lemon lemongrass essential oil: cinnamon bark essential oil = 1-3: 1-4, and rose lemongrass essential oil: cinnamon bark essential oil = 1-3: 1-4.

[0013] More preferably, the composition comprises, by volume ratio: lemongrass essential oil: cinnamon bark essential oil = 1-2: 1-4, lemongrass essential oil: cinnamon bark essential oil = 1-2: 3-4, and rose lemongrass essential oil: cinnamon bark essential oil = 1-3: 3-4.

[0014] More preferably, the composition comprises, by volume ratio: lemongrass essential oil: cinnamon bark essential oil = 1:1, lemongrass essential oil: cinnamon bark essential oil = 2:3, lemongrass essential oil: cinnamon bark essential oil = 1:4, lemon lemongrass essential oil: cinnamon bark essential oil = 2:3, lemon lemongrass essential oil: cinnamon bark essential oil = 1:4, rose lemongrass essential oil: cinnamon bark essential oil = 2:3, and rose lemongrass essential oil: cinnamon bark essential oil = 1:4.

[0015] More preferably, the composition comprises, by volume ratio: lemongrass essential oil: cinnamon bark essential oil = 1:1, lemongrass essential oil: cinnamon bark essential oil = 2:3, and rose lemongrass essential oil: cinnamon bark essential oil = 1:4.

[0016] Essential oils (EOs) are volatile, oily liquids with a distinctive aroma found in aromatic plants. As volatile natural compounds formed from secondary metabolites, they have significant therapeutic effects in clinical applications. They also possess anti-inflammatory, analgesic, antibacterial, hemostatic, and antimicrobial properties, offering excellent protection for dental and oral health. With wide availability and high medicinal value, adding essential oils to toothpaste can strengthen teeth, reduce inflammation and swelling, and provide other benefits.

[0017] Citronella, belonging to the genus *Citronella* of the Poaceae family, is commonly used in traditional medicine to treat neurological and gastrointestinal disorders. It is a medicinal herb with analgesic, antispasmodic, antibacterial, anti-inflammatory, antipyretic, diuretic, sedative, and antihyperglycemic properties. Three varieties are commonly used for essential oil extraction: lemongrass, citronella semperflorens, and rose lemongrass. The active ingredients in these three lemongrass essential oils exhibit inhibitory effects on microorganisms. (E)-citral and (Z)-citral show significant antibacterial effects; both are isomers of citral and can significantly inhibit the growth and toxin production of molds (such as *Aspergillus flavus* and *Aspergillus niger*), preventing feed spoilage.

[0018] Citronella essential oil is widely used in the food and pharmaceutical industries because it contains active ingredients such as citral, nerol, geraniol acetate, and camphor. It has an inhibitory effect on various bacteria and fungi, such as Staphylococcus aureus and Botrytis cinerea, and can also repel fruit flies and mosquitoes. It is widely used in antibacterial products.

[0019] The main component of lemongrass essential oil, citral, when used in combination with the nonsteroidal anti-inflammatory drug naproxen, can produce anti-inflammatory effects, reduce gastric side effects, and can be used in rodent medications. In addition, lemongrass essential oil and citronella essential oil have antibacterial activity against 9 types of microorganisms, and the combination of the two essential oils has a synergistic effect in inhibiting various bacteria.

[0020] Rose citronella essential oil has a high geraniol content, making it a premium source of geraniol for high-end perfumes and cosmetics. It is also used for pest control, exhibiting a high repellency against *C. elegans*. Rose violet essential oil containing over 80% geraniol shows excellent antibacterial activity against *Bacillus subtilis*.

[0021] The main chemical components of cinnamon are terpenoids and aldehydes, while the volatile substances obtained from cinnamon leaves are alcohols, alkenes, and their oxides. Cinnamon bark has anti-inflammatory, antibacterial, antioxidant, cardiovascular, and immunomodulatory effects. Its main bioactive component, cinnamaldehyde, can effectively inhibit the growth of bacteria, yeast, and filamentous molds by inhibiting ATPase, cell wall biosynthesis, and altering membrane structure and integrity. Cinnamon essential oil and cinnamon extract show significant antibacterial activity against oral pathogens and can be used for the prevention of dental caries and periodontal disease, and the treatment of pulpitis and candidiasis.

[0022] The present invention also provides a composition formulation containing lemongrass essential oil, the composition formulation comprising: the above composition and excipients; the excipients being emulsifiers.

[0023] Preferably, the emulsifier is at least one of Tween20 or Tween80.

[0024] More preferably, the volume ratio of the composition to the excipients is 1:1-5.

[0025] This invention also provides a method for preparing a composition formulation containing lemongrass essential oil, specifically comprising the following steps:

[0026] (1) Mix active ingredient A and active ingredient B to obtain a composition;

[0027] (2) Mix the composition with the excipients thoroughly to obtain the final product.

[0028] Further, in step (2), the composition and excipients are mixed and left to stand overnight, and then vortexed to mix thoroughly.

[0029] The present invention also provides the application of the above-mentioned composition containing lemongrass essential oil and its formulation in the preparation of oral care products.

[0030] The present invention also provides the application of the above-mentioned composition containing lemongrass essential oil and its formulation in the preparation of oral care products that inhibit Streptococcus mutans.

[0031] The present invention also provides the use of the above-mentioned composition containing lemongrass essential oil and its preparation in the preparation of a medicament for the prevention or treatment of dental caries caused by Streptococcus mutans.

[0032] Compared with the prior art, the present invention has the following beneficial effects:

[0033] 1. This invention optimizes the composition by selecting natural products. Through the synergistic effect of citronella essential oil and cinnamon bark essential oil, it has a significant inhibitory effect on Streptococcus mutans. By optimizing the ratio of citronella essential oil and cinnamon bark essential oil, the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) are lower, the bactericidal speed is faster, and the effect is significant.

[0034] 2. The preparation method of the composition of the present invention is simple and easy to operate;

[0035] 3. The composition of the present invention has a significant inhibitory effect on the growth of Streptococcus mutans, and compared with the prior art, it has a lower MIC and MBC for Streptococcus mutans and a rapid bactericidal effect; it can be used to prevent and treat dental caries caused by Streptococcus mutans. Attached Figure Description

[0036] Figure 1 The bactericidal time curves of the optimal formulations in Examples 1-3 against Streptococcus mutans are shown. Detailed Implementation

[0037] It is worth noting that the raw materials used in this invention are all commercially available products, and their sources are not specifically limited.

[0038] The following sources of raw materials are provided as examples:

[0039] Citronella essential oil (Cymbopogon narbus) was purchased from Shanghai Poli Co., Ltd., China, product number PEC1903;

[0040] Lemongrass essential oil (Cymbopogon citratus) was purchased from Shanghai Poli Co., Ltd., China, product number PEL0701;

[0041] Rose lemongrass essential oil (Cymbopogon khasans) was purchased from Shanghai Poli Co., Ltd., China, product number PEJ0101;

[0042] Cinnamomum zeylanicum essential oil was purchased from Shanghai Poli Co., Ltd., China, product number PEC160.

[0043] Streptococcus mutans was purchased from the Microbial Culture Collection Center of the Chinese Academy of Sciences; accession number: ATCC700610.

[0044] Example 1

[0045] Using lemongrass essential oil and cinnamon bark essential oil as raw materials, corresponding compositions were prepared according to the proportions in Table 1.

[0046] Table 1. Formulation of lemongrass essential oil and cinnamon bark essential oil combination.

[0047]

[0048] Example 2

[0049] Using lemongrass essential oil and cinnamon bark essential oil as raw materials, corresponding compositions were prepared according to the proportions in Table 2.

[0050] Table 2. Formulation of Lemongrass Essential Oil and Cinnamon Bark Essential Oil Combinations

[0051]

[0052] Example 3

[0053] Using rose lemongrass essential oil and cinnamon bark essential oil as raw materials, corresponding compositions were prepared according to the proportions in Table 3.

[0054] Table 3. Formulation of Rose Citronella Oil and Cinnamon Bark Oil Combinations

[0055]

[0056] Test Example 1: Antibacterial Activity Test of the Composition

[0057] (1) Preparation of culture medium:

[0058] Preparation of liquid culture medium: Weigh 37g of Brain Heart Infusion Broth (BHI) culture medium into 1L of pure water, stir well, dispense into Erlenmeyer flasks, and seal with sealing film; sterilize at 120℃ for 20min before use.

[0059] Preparation of solid culture medium: Weigh 37g of Brain Heart Infusion Broth (BHI) culture medium into 1L of pure water, stir well, dispense into Erlenmeyer flasks, add 1.5g of agar to every 100mL of liquid culture medium, and seal with sealing film; sterilize at 120℃ for 20min, and after the temperature drops below 50℃, dispense into autoclaved petri dishes (perform this operation on a laminar flow hood) and cool for later use.

[0060] (2) Preparation of bacterial suspension:

[0061] Single colonies of *Streptococcus mutans* were inoculated onto nutrient agar and incubated in an anaerobic incubator at 37°C for 16 hours. Activated bacterial colonies were picked and streaked onto nutrient agar for 24 hours. The colonies were washed off with sterile water, shaken thoroughly, and the bacterial count was determined using the plate count method. The resulting culture was then prepared as a 10⁻¹⁰ plate. 6 -10 7 A suspension of CFU / mL.

[0062] (3) Measurement of the diameter of the inhibition zone

[0063] The diameter of the inhibition zone was measured using the filter paper disc method. 200 μL of the prepared bacterial suspension (2 × 10⁻⁶) was taken. 6 The CFU / mL concentration of the test essential oil was evenly spread on BHI agar medium. Then, three 6mm diameter sterile filter paper discs were placed in each dish, and 3μL of the test essential oil or composition was dropped into the center of each disc. An equal volume of sterile water was used as a blank control. The mixture was incubated at 37℃ for 24 hours, and the diameter of the inhibition zone was measured. Each essential oil or composition was measured three times, and the average value was taken. The criterion for determining the inhibition zone was the inhibition index calculated using chlorhexidine as a positive control.

[0064] The inhibitory effects of chlorhexidine, lemongrass essential oil, citronella essential oil, rose lemongrass essential oil, cinnamon bark essential oil, and the compositions of Examples 1-3 on Streptococcus mutans were tested using the above method. The antibacterial results of chlorhexidine, lemongrass essential oil, citronella essential oil, rose lemongrass essential oil, and cinnamon bark essential oil are shown in Table 5, and the antibacterial results of the compositions of Examples 1-3 are shown in Tables 6-8. The test results show that, under the same dosage of the tested essential oils and compositions, compared with the control chlorhexidine (which has a fairly strong broad-spectrum antibacterial and bactericidal effect against a variety of bacteria, but is prone to causing allergies and irritation; the inhibition zone diameter is 18.2±0.15 mm), all the essential oils produced inhibition zones of varying sizes. For single essential oils, cinnamon bark essential oil showed the best antibacterial effect, while the other essential oils showed comparable antibacterial effects. Based on the performance of the compositions, the 2:3 ratio of lemongrass essential oil and cinnamon bark essential oil showed the best antibacterial effect (inhibition zone diameter was 49.90±1.21mm); followed by the 1:4 ratio of rose lemongrass essential oil and cinnamon bark essential oil (inhibition zone diameter was 47.67±2.37mm) and the 1:1 ratio of lemongrass essential oil and cinnamon bark essential oil (inhibition zone diameter was 43.60±0.87mm). This indicates that lemongrass essential oil and cinnamon bark essential oil, when formulated in specific ratios, exhibit a synergistic effect in inhibiting Streptococcus mutans.

[0065] Table 5. Inhibition results of chlorhexidine, lemongrass, lemon citronella, rose stigma, cinnamon bark, and palmarosa on Streptococcus mutans.

[0066]

[0067]

[0068] Table 6. Inhibition results of the lemongrass + cinnamon bark combination against Streptococcus mutans.

[0069]

[0070] Table 7. Inhibition results of the lemongrass + cinnamon bark combination against Streptococcus mutans.

[0071]

[0072] Table 8. Inhibition results of the combination of lemongrass and cinnamon bark on Streptococcus mutans.

[0073]

[0074] Test Example 2: Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) Test

[0075] The MIC of the composition with good antibacterial activity against Streptococcus mutans was determined using the micro-broth dilution method. Since the essential oil is insoluble in water, it was mixed with 1:1 (v / v) DMSO:Tween 20 and BHI liquid medium (as an emulsifier to promote contact with bacteria). This resulted in a final concentration of 0.04-5 μL / mL of essential oil. A solution of the known concentration of essential oil was added to 1×10⁻⁶ mol / L broth. 6 The bacterial suspension was cultured in a shaker at 37°C for 24 h at CFU / mL. 1% chlorhexidine and BHI medium containing 0.1% emulsifier served as negative and positive controls, respectively. The minimum inhibitory concentration (MIC) was defined as the concentration of essential oil that visually inhibited bacterial growth in the culture medium. Bacterial growth was expressed as turbidity.

[0076] After determining the MIC, 100 μL of each of the minimum inhibitory concentration (MIC) bacterial suspension and the bacterial suspension above the MIC were plated onto BHI agar plates and anaerobically incubated at 37°C for 24 h. The concentration of essential oil corresponding to the bacterial suspension in which no colonies were observed to grow in the solid medium was taken as the MBC value. The MBC value was measured three times.

[0077] The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of the single essential oils lemongrass oil, lemongrass oil, rose lemongrass oil, and cinnamon bark oil, as well as the compositions of Example 1 (lemongrass oil: cinnamon bark oil = 1:1), Example 2 (lemongrass oil: cinnamon bark oil = 2:3), and Example 3 (rose lemongrass oil: cinnamon bark oil = 1:4) are shown in Table 9. The MIC and MBC of the compound essential oils of the present invention are twice that of chlorhexidine, which also has a significant antibacterial effect. Furthermore, the MIC and MBC of lemongrass oil + cinnamon bark oil, lemongrass oil + cinnamon bark oil, and rose lemongrass oil + cinnamon bark oil are all lower than those of any single essential oil, indicating that the combination of lemongrass oil, lemongrass oil, rose lemongrass oil, and cinnamon bark oil of the present invention has a stronger antibacterial effect, and there is a significant synergistic effect between lemongrass essential oil and cinnamon bark oil.

[0078] Table 9. Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC)

[0079]

[0080] Test Example 3: Kill Time Test

[0081] The minimum bactericidal concentration of the composition was determined using the agar dilution method, and the corresponding time-bactericidal curves were measured to reflect the bactericidal effect of the MBC concentration composition at different time points. Streptococcus mutans (1×10⁻⁶) was cultured in BHI medium. 6The bacterial suspension (CFU / mL) was incubated at MBC concentration in a shaker at 37°C and 180 rpm. Every 1 day, a portion of the bacterial suspension was spread onto solid BHI agar and incubated for 24 hours. The number of single colonies in the medium was recorded. The experiment was repeated three times, and the average value was calculated. A sterilization time curve was plotted with incubation time on the x-axis and the natural logarithm of the viable cell count on the y-axis.

[0082] The time-bacterial curves of the compositions against Streptococcus mutans within 10 hours using the blank group (i.e., suspension without added essential oils and the composition) at a final concentration of MBC, the control group (chlorhexidine), and the experimental groups (Example 1:citronella essential oil: cinnamon bark essential oil = 1:1), Example 2: lemongrass essential oil: cinnamon bark essential oil = 2:3), and Example 3: rose lemongrass essential oil: cinnamon bark essential oil = 1:4) are shown below. Figure 1 .Depend on Figure 1 It can be seen that the number of Streptococcus mutans in the control group gradually increased throughout the entire culture period. The composition maintained a continuous bactericidal effect on Streptococcus mutans, and the number of bacteria killed increased with the extension of time, with the bactericidal effect slightly enhanced as time increased. The MBC values ​​of the compositions all showed partial bactericidal effects in a short period of time. Among them, the antibacterial effects of lemongrass essential oil + cinnamon bark essential oil and lemon lemongrass essential oil + cinnamon bark essential oil were comparable to chlorhexidine, and they could effectively inhibit the growth of Streptococcus mutans within 6 hours. Rose lemongrass essential oil + cinnamon bark essential oil could effectively inhibit the growth of Streptococcus mutans within 8 hours.

[0083] In summary, the compositions of this invention exhibit superior antibacterial effects against Streptococcus mutans through the synergistic effect of lemongrass, cinnamon bark, and palmarosa in specific ratios. The compositions with a 2:3 ratio of lemongrass essential oil and cinnamon bark essential oil, and a 1:4 ratio of rose lemongrass essential oil and cinnamon bark essential oil, show the best antibacterial effects against Streptococcus mutans, with inhibition zone diameters reaching 49.90±1.21 mm and 47.67±2.37 mm, respectively. The compositions of the three lemongrass essential oils and cinnamon bark essential oil have comparable minimum inhibitory concentrations (MICs) and minimum bactericidal concentrations (MBCs) against Streptococcus mutans. At the MBC level, the composition of lemongrass essential oil and lemongrass essential oil with cinnamon bark essential oil shows better bactericidal effects, effectively inhibiting the growth of Streptococcus mutans within 6 hours, comparable to chlorhexidine. The composition of rose lemongrass essential oil and cinnamon bark essential oil is the second best, inhibiting the growth of Streptococcus mutans within 8 hours.

[0084] Finally, it should be noted that the above content is only used to illustrate the technical solution of the present invention, and is not intended to limit the scope of protection of the present invention. Simple modifications or equivalent substitutions made by those skilled in the art to the technical solution of the present invention do not depart from the essence and scope of the technical solution of the present invention.

Claims

1. A composition containing lemongrass essential oil, characterized in that, The composition comprises active ingredient A and active ingredient B; active ingredient A is at least one selected from lemongrass essential oil, lemon lemongrass essential oil, and rose lemongrass essential oil; active ingredient B is cinnamon bark essential oil; The composition, by volume ratio, is: lemongrass essential oil: cinnamon bark essential oil = 3:2-1:4, lemon lemongrass essential oil: cinnamon bark essential oil = 3:2-1:4, and rose lemongrass essential oil: cinnamon bark essential oil = 3:2-1:

4.

2. A compositional formulation containing lemongrass essential oil, characterized in that, The composition formulation comprises: the composition of claim 1 and an excipient; the excipient is an emulsifier.

3. The composition formulation according to claim 2, characterized in that, The emulsifier is at least one of Tween20 and Tween80.

4. The composition formulation according to claim 2, characterized in that, The volume ratio of the composition to the excipients is 1:1-5.

5. The method for preparing the composition formulation according to any one of claims 2-4, characterized in that, Specifically, the following steps are included: (1) Mix active ingredient A and active ingredient B to obtain a composition; (2) The composition is thoroughly mixed with the excipients to obtain the composition formulation.

6. The use of the composition according to claim 1 or the composition formulation according to any one of claims 2-4 in the preparation of oral care products.

7. The use of the composition according to any one of claims 1 or the composition formulation according to any one of claims 2-4 in the preparation of oral care products that inhibit Streptococcus mutans.

8. The use of the composition according to any one of claims 1 or the composition formulation according to any one of claims 2-4 in the preparation of a medicament for the prevention or treatment of dental caries caused by Streptococcus mutans.

Images