Anthrax spore marker fluorescence-ultraviolet dual-mode detection method

Abstract

This invention relates to the field of analytical chemistry, specifically providing a fluorescence-UV dual-mode detection probe, comprising a fluorescence mode detection probe and a UV mode detection probe, wherein the probe comprises CdTe quantum dots and xylenol orange; providing the application of the fluorescence-UV dual-mode detection probe in the detection of anthrax spore markers; and providing a fluorescence-UV dual-mode detection method for anthrax spore markers: mixing CdTe quantum dot dispersion and xylenol orange solution in a certain proportion to prepare fluorescence mode detection probe and UV mode detection probe respectively; taking the sample to be tested, adding the fluorescence mode detection probe or the UV mode detection probe, and measuring the concentration of the analyte in fluorescence mode or UV mode. The results show that after adding the anthrax spore marker, the emission peak at 635 nm is enhanced in fluorescence mode; in UV detection mode, the absorption peak at 434 nm is enhanced, and the absorption peak at 578 nm is weakened.

Description

Technical Field

[0001] This invention relates to the field of analytical chemistry, and in particular to a fluorescence-ultraviolet dual-mode detection method for anthrax bacillus spore markers. Background Technology

[0002] 2,6-Pyridinedicarboxylic acid (DPA) accounts for 5%-15% of the dry weight of Bacillus anthracis spores and is a biomarker for these spores. Bacillus anthracis spores are transformed from active Bacillus anthracis under extreme conditions such as high temperature, freezing, ultraviolet radiation, drying, strong acid, and strong alkali. Bacillus anthracis spores have a strong ability to survive in extreme environments and, under suitable conditions, transform into active Bacillus anthracis, releasing DPA in the process. Humans can become infected with anthrax through contact with livestock, water, air, and soil contaminated with Bacillus anthracis spores. For example, a person who inhales more than 10,000 Bacillus anthracis spores will die within 24-36 hours without effective treatment. Due to the immense harm caused by Bacillus anthracis spores, detecting its biomarker DPA is crucial for early identification and treatment.

[0003] Currently, methods for detecting DPA include high-performance liquid chromatography (HPLC), Raman spectroscopy, electrochemical methods, fluorescence methods, and ultraviolet (UV) methods. Among these, fluorescence and UV detection methods have received widespread attention due to their advantages of speed, simplicity, intuitiveness, and low cost. Both fluorescence and UV methods are generally based on the interaction of DPA with metal ions (Ca). 2+ Zn 2+ Cu 2+ Eu 3+ Detection is achieved through the coordination of (etc.). For example, Lei Jia et al. synthesized fluorescein and Eu... 3+ The composite material was used for the fluorescence detection of DPA. The green fluorescence of fluorescein was not affected by DPA, while the fluorescence of DPA and Eu... 3+ Coordination sensitization of Eu 3+ The red fluorescence (J. Hazard. Mater., 2021, 402, 123776). For example, Mirza Muhammad et al. developed a UV method based on glutathione-functionalized gold nanoparticles and calcium ions for the detection of DPA, Ca... 2+ Coordination with glutathione causes gold nanoparticles to aggregate, changing their color from red to purple. DPA can also interact with Ca... 2+Coordination leads to the disaggregation of gold nanoparticles. As mentioned above, reported methods are typically single-mode fluorescence or ultraviolet methods. The drawback of single-mode detection is its susceptibility to interference from environmental and instrument fluctuations, resulting in insufficient accuracy. In contrast, the fluorescence-ultraviolet dual-mode detection method provides two usable readout signals, enabling calibration and verification between different detection modes, ensuring detection accuracy, and demonstrating greater convenience in practical applications. Therefore, the development of the fluorescence-ultraviolet dual-mode detection method for DPA has attracted widespread attention from researchers. Summary of the Invention

[0004] To address the aforementioned problems, this invention provides a fluorescence-ultraviolet dual-mode detection probe and detection method for anthrax spore markers.

[0005] The primary objective of this invention is to provide a fluorescence-ultraviolet dual-mode detection method for anthrax bacillus spore markers, specifically comprising the following steps:

[0006] S1. Preparation of CdTe quantum dot dispersion;

[0007] S2. Add xylenol orange to water to prepare a 1 mM xylenol orange solution;

[0008] S3. Add the CdTe quantum dot dispersion and xylenol orange solution to a buffer solution at a volume ratio of 1:3-5, mix, and prepare a fluorescence mode detection probe.

[0009] S4. The CdTe quantum dot dispersion and xylenol orange solution are added to a buffer solution at a volume ratio of 1:1 to 6, mixed, and a UV mode detection probe is prepared.

[0010] S5. Take the sample to be tested, add the fluorescence mode detection probe described in step S3, and determine the concentration of anthrax spore markers at an excitation wavelength of 286 nm and an emission wavelength of 635 nm.

[0011] S6. Take the sample to be tested, add the UV mode detection probe described in step S4, let it stand at room temperature for 1-3 minutes, and determine the concentration of anthrax spore markers at a UV absorption wavelength of 434nm-578nm.

[0012] Preferably, in step S3, the volume ratio of CdTe quantum dot dispersion to xylenol orange solution is 1:3, and in step S4, the volume ratio of CdTe quantum dot dispersion to xylenol orange solution is 1:1.

[0013] Preferably, the method for preparing the CdTe quantum dot dispersion in step S1 includes:

[0014] S11. At room temperature, dissolve 0.2-0.3 g sodium citrate dihydrate, 0.1-0.2 g cadmium acetate dihydrate and 50-60 μL 3-mercaptopropionic acid in 100 mL of water, and adjust the pH to 10-11 with 1 M sodium hydroxide aqueous solution under stirring.

[0015] S12, add 0.02-0.03g sodium tellurite and 0.05-0.06g sodium borohydride, stir for 8-12 minutes, reflux at 95-100℃ for 8.5-9.5 hours, and cool to room temperature;

[0016] S13. Place in a 3500Da dialysis bag and dialyze in water for 20-30 hours to obtain a CdTe quantum dot dispersion.

[0017] Preferably, the buffer solution is a 10 mM HEPES buffer solution with a pH of 6.0.

[0018] A second objective of this invention is to provide a fluorescence-ultraviolet dual-mode detection probe, comprising a fluorescence mode detection probe and an ultraviolet mode detection probe, wherein the probe comprises CdTe quantum dots and xylenol orange.

[0019] Preferably, the CdTe quantum dots are a CdTe quantum dot dispersion, and the xylenol orange is a 1 mM xylenol orange solution.

[0020] Preferably, the fluorescence mode detection probe is prepared by mixing the CdTe quantum dot dispersion and xylenol orange solution with a buffer solution at a volume ratio of 1:3 to 5; the ultraviolet mode detection probe is prepared by mixing the CdTe quantum dot dispersion and xylenol orange solution with a buffer solution at a volume ratio of 1:1 to 6.

[0021] Preferably, the volume ratio of CdTe quantum dot dispersion to xylenol orange solution in the fluorescence mode detection probe is 1:3; and the volume ratio of CdTe quantum dot dispersion to xylenol orange solution in the ultraviolet mode detection probe is 1:1.

[0022] Preferably, the method for preparing the CdTe quantum dot dispersion includes:

[0023] S11. At room temperature, dissolve 0.2-0.3 g sodium citrate dihydrate, 0.1-0.2 g cadmium acetate dihydrate and 50-60 μL 3-mercaptopropionic acid in 100 mL of water, and adjust the pH to 10-11 with 1 M sodium hydroxide aqueous solution under stirring.

[0024] S12, add 0.02-0.03g sodium tellurite and 0.05-0.06g sodium borohydride, stir for 8-12 minutes, reflux at 95-100℃ for 8.5-9.5 hours, and cool to room temperature;

[0025] S13. Place in a 3500Da dialysis bag and dialyze in water for 20-30 hours to obtain a CdTe quantum dot dispersion.

[0026] A third objective of this invention is to provide the application of a fluorescence-ultraviolet dual-mode detection probe in the detection of anthrax spore markers.

[0027] Preferably, the anthrax bacillus spore marker is 2,6-pyridinedicarboxylic acid.

[0028] Beneficial effects of this invention:

[0029] This invention relates to a probe for anthrax spore markers based on CdTe quantum dots functionalized with xylenol orange. By changing the ratio of CdTe quantum dots to xylenol orange, it allows switching between fluorescence and ultraviolet (UV) detection modes. In fluorescence detection mode, the addition of the anthrax spore marker enhances the emission peak at 635 nm. In UV detection mode, the addition of the anthrax spore marker enhances the absorption peak at 434 nm and weakens the absorption peak at 578 nm. The probe can detect anthrax spore markers in both fluorescence and UV modes, making it more convenient in practical applications. Furthermore, the results from the two detection modes can be cross-validated, resulting in higher accuracy. Attached Figure Description

[0030] Figure 1 This is a transmission electron microscope image of the CdTe quantum dots of this invention;

[0031] Figure 2 This is the X-ray photoelectron spectrum of the CdTe quantum dots of this invention;

[0032] Figure 3 This is the X-ray photoelectron spectrum of the S element in the CdTe quantum dots of this invention;

[0033] Figure 4 This is the X-ray photoelectron spectrum of Cd element in CdTe quantum dots before and after the addition of xylenol orange according to the present invention;

[0034] Figure 5 This is a transmission electron microscope image of CdTe quantum dots after the addition of xylenol orange according to the present invention;

[0035] Figure 6 These are the fluorescence spectra of the present invention after adding different concentrations of DPA;

[0036] Figure 7 This is the standard curve for the fluorescence detection mode of this invention;

[0037] Figure 8 These are the absorption spectra of the present invention after adding different concentrations of DPA;

[0038] Figure 9This is the standard curve for the ultraviolet detection mode of this invention;

[0039] Figure 10 This invention relates to the effect of the volume of xylenol orange solution on the sensitivity of DPA fluorescence detection.

[0040] Figure 11 This invention relates to the effect of the volume of the CdTe quantum dot dispersion on the sensitivity of DPA ultraviolet detection.

[0041] Figure 12 This is a schematic diagram of the reaction mechanism of the present invention.

[0042] Figure 13 This is the X-ray photoelectron spectrum of Cd element in CdTe quantum dots before and after the addition of DPA in this invention. Detailed Implementation

[0043] To make the objectives, technical solutions, and advantages of this invention clearer, the invention will be further described in detail below with reference to the accompanying drawings and specific embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and do not constitute a limitation thereof.

[0044] Example 1

[0045] I. Preparation of CdTe quantum dot dispersion

[0046] CdTe quantum dots were prepared according to the method described in the literature (J. Mater. Chem., 2012, 22, 14573-14578). Sodium citrate dihydrate (0.23 g), cadmium acetate dihydrate (0.13 g), and 3-mercaptopropionic acid (53 μL) were dissolved in 100 mL of water at room temperature. The pH was adjusted to 10.5 with a 1M sodium hydroxide aqueous solution under stirring. Then, sodium tellurite (0.023 g) and sodium borohydride (0.055 g) were added, and the resulting solution was stirred for 10 min, followed by reflux at 100 °C for 9 h. After cooling the reaction solution to room temperature, it was dialyzed in water for 24 h using a 3500 Da dialysis bag to obtain a CdTe quantum dot dispersion. Figure 1 As shown, transmission electron microscopy reveals that the CdTe quantum dots are spherical with a lattice spacing of 0.25 nm. Figure 2 As shown, X-ray photoelectron spectroscopy indicates that the CdTe quantum dots contain the elements Te, O, Cd, C, and S. Figure 3 As shown, the X-ray photoelectron spectrum of S element indicates that S 2p in CdTe quantum dots 1 / 2 and S 2p 3 / 2 The binding energies are located at 168.8 eV and 167.6 eV, respectively, proving that the thiol group in 3-mercaptopropionic acid binds to the CdTe core.

[0047] II. Preparation of Cresol Orange Solution

[0048] Dissolve 0.067 g of xylenol orange in 100 mL of water at room temperature to obtain a 1 mM xylenol orange solution.

[0049] Example 2

[0050] Preparation of probe solutions: For the fluorescence detection mode, at room temperature, 50 μL of CdTe quantum dot dispersion and 150 μL of xylenol orange solution were mixed in 3600 μL of HEPES buffer solution (10 mM, pH 6.0) to prepare the probe solution for the fluorescence detection mode; for the ultraviolet detection mode, at room temperature, 150 μL of CdTe quantum dot dispersion and 150 μL of xylenol orange solution were mixed in 3500 μL of HEPES buffer solution (10 mM, pH 6.0) to prepare the probe solution for the ultraviolet detection mode.

[0051] like Figure 4 As shown, X-ray photoelectron spectroscopy indicates that the addition of xylenol orange alters the binding energy of Cd 3d in CdTe quantum dots, suggesting that xylenol orange binds to Cd 3d ions through Xylenol orange. 2+ The coordination effect is modified onto CdTe quantum dots. For example... Figure 5 As shown, after modification with xylenol orange, the CdTe quantum dots still exhibit a spherical shape with a lattice spacing of 0.25 nm, indicating that the modification with xylenol orange has no effect on the morphology and structure of the CdTe quantum dots.

[0052] Example 3

[0053] I. Fluorescence mode detection

[0054] Establishing a standard curve: 200 μL of DPA aqueous solutions of different concentrations were added to the prepared probe solution. The DPA concentrations in the prepared solutions were 0, 0.1, 0.5, 1, 2, 3, 4, and 5 μM. After the prepared solutions were allowed to stand at room temperature for 1 minute, their fluorescence intensity was measured using a fluorescence spectrophotometer. The excitation wavelength was 286 nm, the emission wavelength was 635 nm, and the cuvette size was 1 cm. Next, the (F / F0) ratio was used... 4 A standard curve was constructed with (F - fluorescence intensity with DPA, F0 - fluorescence intensity without DPA) on the ordinate and DPA concentration on the x-axis. Figure 6 As shown, the fluorescence intensity at 635 nm increases with increasing DPA concentration. Figure 7 As shown, when the concentration of DPA is in the range of 0.1-5 μM, (F / F0) 4 The concentration of DPA showed a linear relationship, and the linear fitting equation was y = 3.323x + 0.807(R²). 2 =0.9925).

[0055] Determination of unknown sample: At room temperature, 50 μL of CdTe quantum dot dispersion and 150 μL of xylenol orange solution were mixed in 3600 μL of HEPES buffer solution (10 mM, pH 6.0), and then 200 μL of unknown sample was added. After the prepared solution was allowed to stand at room temperature for 1 minute, the fluorescence intensity was measured using a fluorescence spectrophotometer with an excitation wavelength of 286 nm and an emission wavelength of 635 nm. The UV dish size was 1 cm. The concentration of DPA was then calculated based on the established standard curve.

[0056] II. Ultraviolet Mode Detection

[0057] Establishing a standard curve: At room temperature, 150 μL of CdTe quantum dot dispersion and 150 μL of xylenol orange solution were mixed in 3500 μL of HEPES buffer solution (10 mM, pH 6.0), followed by the addition of 200 μL of DPA aqueous solutions of different concentrations. The DPA concentrations in the prepared solutions were 0, 0.5, 1, 2, 4, 6, 8, 10, 15, 20, 25, 30, and 40. After allowing the prepared solutions to stand at room temperature for 1 minute, the absorbance was measured using a UV-Vis spectrophotometer. Two absorption wavelengths were used: 434 nm and 578 nm. The UV dish was 1 cm in size. Next, lg(A) was used to measure the absorbance. 434 / A 578 (A) 434 Absorbance at -434 nm, A 578 A standard curve was constructed using the absorbance at -578 nm as the ordinate and the concentration of DPA as the abscissa. Figure 8 As shown, with increasing DPA concentration, the absorbance at 434 nm gradually increases, while the absorbance at 578 nm gradually decreases. Figure 9 As shown, when the concentration of DPA is in the range of 0.5-40 μM, lg(A) 434 / A 578 The concentration of DPA showed a linear relationship with the concentration of y, and the linear fitting equation was y = 0.013x + 0.172(R²). 2 =0.9952).

[0058] Determination of the unknown sample: At room temperature, 150 μL of CdTe quantum dot dispersion and 150 μL of xylenol orange solution were mixed in 3500 μL of HEPES buffer solution (10 mM, pH 6.0), and then 200 μL of the unknown sample was added. After the prepared solution was allowed to stand at room temperature for 1 minute, the absorbance was measured using a UV-Vis spectrophotometer. Two absorption wavelengths were used: 434 nm and 578 nm. The UV dish was 1 cm in size. The concentration of DPA was then calculated based on the established standard curve.

[0059] Example 4

[0060] The ratio of CdTe quantum dots to xylenol orange was optimized, specifically including the following steps:

[0061] I. Optimize the addition volume of xylenol orange

[0062] The volume of xylenol orange solution affects the fluorescence spectrum during probe solution preparation. Therefore, the volume of xylenol orange solution was optimized, and the specific steps are as follows: At room temperature, 50 μL of CdTe quantum dot dispersion was mixed with different volumes of xylenol orange solution (50, 75, 100, 150, 200, 250 μL), and then HEPES buffer solution (10 mM, pH 6.0) was added to a total volume of 3800 μL to prepare the probe solution. Next, 200 μL of water or 200 μL of DPA aqueous solution was added to the prepared probe solution. The concentration of DPA in the prepared solution was 0 μM or 10 μM. After the prepared solution was allowed to stand at room temperature for 1 minute, the fluorescence intensity was measured using a fluorescence spectrophotometer, with an excitation wavelength of 286 nm, an emission wavelength of 635 nm, and a cuvette size of 1 cm. Figure 10 As shown, when the volume of xylenol orange solution increased from 50 μL to 150 μL, the fluorescence detection sensitivity of DPA increased; when the volume of xylenol orange solution increased from 150 μL to 250 μL, the fluorescence detection sensitivity of DPA remained basically unchanged.

[0063] II. Optimizing the volume of CdTe quantum dot dispersion

[0064] In the preparation of the probe solution, the volume of the CdTe quantum dot dispersion affects the absorption spectrum. Therefore, the volume of the CdTe quantum dot dispersion was optimized, and the specific steps are as follows: At room temperature, different volumes of CdTe quantum dot dispersion (25, 50, 75, 100, 150, 200, 250, 300 μL) were mixed with 150 μL of xylenol orange solution, and HEPES buffer solution (10 mM, pH 6.0) was added to a total volume of 3800 μL to prepare the probe solution. 200 μL of water or 200 μL of DPA aqueous solution was added to the probe solution. The concentration of DPA in the prepared solution was 0 μM or 30 μM. After the prepared solution was allowed to stand at room temperature for 1 minute, the absorbance was measured using a UV-Vis spectrophotometer. Two absorption wavelengths were observed: 434 nm and 578 nm. The UV dish was 1 cm in size. Figure 11 As shown, when the volume of the CdTe quantum dot dispersion increased from 25 μL to 150 μL, the UV detection sensitivity of DPA remained basically unchanged (with a very small downward trend), while when the volume of the CdTe quantum dot dispersion increased from 150 μL to 300 μL, the fluorescence detection sensitivity of DPA decreased.

[0065] Example 5

[0066] Dual-mode detection probe for urine: A series of spiked urine samples were prepared by adding different amounts of DPA to the urine, and each spiked sample was diluted 30 times with water for detection. At room temperature, 50 μL of CdTe quantum dot dispersion was mixed with 150 μL of xylenol orange solution in 3600 μL of HEPES buffer solution (10 mM, pH 6.0), and then 200 μL of spiked urine sample was added. After the prepared solution was allowed to stand at room temperature for 1 minute, the fluorescence intensity was measured using a fluorescence spectrophotometer with an excitation wavelength of 286 nm and an emission wavelength of 635 nm. The UV dish size was 1 cm. The concentration of DPA was then calculated based on the established standard curve. As shown in Table 1, the relative standard deviation of the detection results was in the range of 0.1%–10.2%, and the spiked recovery rate of DPA in urine was in the range of 100.0%–115.0%, indicating that the fluorescence mode has high precision and accuracy for detecting DPA in urine.

[0067] Table 1. Detection results of fluorescence patterns of DPA in urine.

[0068]

[0069] Different amounts of DPA were added to urine to prepare a series of spiked urine samples, which were then diluted 30-fold with water for detection. At room temperature, 150 μL of CdTe quantum dot dispersion and 150 μL of xylenol orange solution were mixed in 3500 μL of HEPES buffer (10 mM, pH 6.0), and then 200 μL of spiked urine sample was added. After the prepared solution was allowed to stand at room temperature for 1 minute, the absorbance was measured using a UV-Vis spectrophotometer. Two absorption wavelengths were used: 434 nm and 578 nm. The UV dish was 1 cm in size. The concentration of DPA was then calculated based on the established standard curve. As shown in Table 2, the relative standard deviation of the detection results was in the range of 0.8%–1.8%, and the spiked recovery rate of DPA in urine was in the range of 86.0%–96.6%, indicating that the fluorescence mode has high precision and accuracy for the detection of DPA in urine.

[0070] Table 2 Results of UV detection of DPA in urine

[0071]

[0072] This embodiment simulates the practical application of a fluorescence-ultraviolet dual-mode detection probe in detecting anthrax spore markers in samples.

[0073] Example 6

[0074] The anthrax bacillus spore marker fluorescence-ultraviolet dual-mode detection method provided by this invention has the following schematic diagram of its reaction mechanism: Figure 12 .

[0075] The mechanism investigation and results are as follows: At room temperature, 0.3 mL of CdTe quantum dot dispersion was added to 2.25 mL of HEPES buffer solution (10 mM, pH 6.0), followed by the addition of 0.45 mL of water or 0.45 mL of DPA aqueous solution. The concentration of DPA in the prepared solutions was either 0 μM or 300 μM. The prepared solutions were then characterized using X-ray photoelectron spectroscopy. Figure 13 As shown, X-ray photoelectron spectroscopy indicates that the addition of DPA alters the binding energy of Cd 3d in CdTe quantum dots, suggesting that DPA can bind with Cd in CdTe quantum dots. 2+ This leads to coordination. Both DPA and xylenol orange can react with Cd in CdTe quantum dots. 2 + Through coordination, DPA can replace xylenol orange modified on CdTe quantum dots, resulting in changes in fluorescence and absorption spectra, enabling dual-mode detection of DPA in both fluorescence and ultraviolet light.

[0076] Although embodiments of the present invention have been shown and described above, it is to be understood that the above embodiments are exemplary and should not be construed as limiting the present invention. Those skilled in the art can make changes, modifications, substitutions, and variations to the above embodiments within the scope of the present invention.

[0077] The specific embodiments of the present invention described above do not constitute a limitation on the scope of protection of the present invention. Any other corresponding changes and modifications made in accordance with the technical concept of the present invention should be included within the scope of protection of the claims of the present invention.

Claims

1. A method for detecting anthrax bacillus spore markers using a fluorescence-ultraviolet dual-mode approach, characterized in that: Specifically, the steps include the following: S1. Preparation of CdTe quantum dot dispersion; S2. Add xylenol orange to water to prepare a 1 mM xylenol orange solution; S3. Add the CdTe quantum dot dispersion and xylenol orange solution to a buffer solution at a volume ratio of 1:3~5, mix, and prepare a fluorescence mode detection probe. S4. The CdTe quantum dot dispersion and xylenol orange solution are added to a buffer solution at a volume ratio of 1:1~6, mixed, and a UV mode detection probe is prepared. S5. Take the sample to be tested, add the fluorescence mode detection probe described in step S3, and determine the concentration of anthrax spore markers at an excitation wavelength of 286 nm and an emission wavelength of 635 nm. S6. Take the sample to be tested, add the UV mode detection probe described in step S4, let it stand at room temperature for 1-3 minutes, and determine the concentration of anthrax spore markers at a UV absorption wavelength of 434nm~578nm.

2. The method for detecting anthrax bacillus spore markers in fluorescence-ultraviolet dual mode according to claim 1, characterized in that: In step S3, the volume ratio of CdTe quantum dot dispersion to xylenol orange solution is 1:3, and in step S4, the volume ratio of CdTe quantum dot dispersion to xylenol orange solution is 1:

1.

3. The method for detecting anthrax bacillus spore markers in fluorescence-ultraviolet dual mode according to claim 2, characterized in that: The preparation method of the CdTe quantum dot dispersion in step S1 includes: S11. At room temperature, dissolve 0.2~0.3g sodium citrate dihydrate, 0.1~0.2g cadmium acetate dihydrate and 50~60μL 3-mercaptopropionic acid in 100mL of water, and adjust the pH to 10~11 with 1M sodium hydroxide aqueous solution under stirring. S12, add 0.02~0.03g sodium tellurite and 0.05~0.06g sodium borohydride, stir for 8~12min, reflux at 95~100℃ for 8.5~9.5h, and cool to room temperature; S13. Place in a 3500Da dialysis bag and dialyze in water for 20-30 hours to obtain a CdTe quantum dot dispersion.

4. The method for detecting anthrax bacillus spore markers in fluorescence-ultraviolet dual mode according to claim 3, characterized in that: The buffer solution is a 10 mM HEPES buffer solution with a pH of 6.

0.

5. A fluorescence-ultraviolet dual-mode detection probe, characterized in that, include: A fluorescence mode detection probe and a UV mode detection probe, wherein the probe comprises CdTe quantum dots and xylenol orange; the fluorescence mode detection probe is prepared by mixing the CdTe quantum dot dispersion and xylenol orange solution in a buffer solution at a volume ratio of 1:3 to 5; the UV mode detection probe is prepared by mixing the CdTe quantum dot dispersion and xylenol orange solution in a buffer solution at a volume ratio of 1:1 to 6.

6. The fluorescence-UV dual-mode detection probe according to claim 5, characterized in that: The CdTe quantum dots are a CdTe quantum dot dispersion, and the xylenol orange is a 1 mM xylenol orange solution.

7. The fluorescence-UV dual-mode detection probe according to claim 5, characterized in that: The volume ratio of CdTe quantum dot dispersion to xylenol orange solution in the fluorescence mode detection probe is 1:3; the volume ratio of CdTe quantum dot dispersion to xylenol orange solution in the ultraviolet mode detection probe is 1:

1.

8. The fluorescence-UV dual-mode detection probe according to claim 7, characterized in that: The method for preparing the CdTe quantum dot dispersion includes: S11. At room temperature, dissolve 0.2~0.3g sodium citrate dihydrate, 0.1~0.2g cadmium acetate dihydrate and 50~60μL 3-mercaptopropionic acid in 100mL of water, and adjust the pH to 10~11 with 1M sodium hydroxide aqueous solution under stirring. S12, add 0.02~0.03g sodium tellurite and 0.05~0.06g sodium borohydride, stir for 8~12min, reflux at 95~100℃ for 8.5~9.5h, and cool to room temperature; S13. Place in a 3500Da dialysis bag and dialyze in water for 20-30 hours to obtain a CdTe quantum dot dispersion.

9. The application of the fluorescence-ultraviolet dual-mode detection probe according to any one of claims 5-8 in the detection of anthrax spore markers.

10. The application according to claim 9, characterized in that: The anthrax bacillus spore marker is 2,6-pyridinedicarboxylic acid.

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