A high-efficiency and low-residue pesticide composition

Abstract

The present invention relates to the technical field of pesticides, and more particularly to a high-efficiency, low-residue pesticide composition, the active ingredients of which are formed by compounding spinetoram with patchouli ketone, wherein the mass ratio of spinetoram to patchouli ketone is 1-40:3-1. When the spinetoram and patchouli ketone are compounded in the pesticide composition of the present invention, a synergistic effect is exhibited within a certain mass ratio range, thereby improving the control effect. This synergistic effect can reduce the application dosage of chemical agents, thereby avoiding, to a certain extent, the problems of pesticide residues and environmental pollution caused by high-dose application.

Description

Technical Field

[0001] The present invention relates to the technical field of pesticides, and in particular to a high-efficiency and low-residue pesticide composition. Background Art

[0002] The fall armyworm (Spodoptera litura Fabricius), belonging to the Noctuidae family of the order Lepidoptera, is also known as the lotus-patterned moth, commonly known as the night thief, and the aconite. It is a omnivorous, voracious pest with a worldwide distribution. Reportedly, the fall armyworm can harm 389 species of host plants belonging to 109 families. The fall armyworm's larvae injure the affected leaves. Young larvae feed on the mesophyll, leaving only a thin, window-like epidermis. Older larvae eat the leaves, leaving notches. In severe cases, the entire leaf is devoured, leaving only the main veins, causing severe losses to agricultural production. Currently, chemical control is the primary method for controlling fall armyworm. In actual production, the use of a single pesticide can easily lead to pest resistance, resulting in reduced control effectiveness. Increasing the dosage of the pesticide can lead to a series of problems, including pesticide residues and environmental pollution.

[0003] Patchouli ketone is a compound obtained by separation and purification from patchouli essential oil with insecticidal activity. It is a colorless needle-shaped crystal with a molecular formula of C 12 H 16 O4, molecular weight is 224.257, and its structural formula is as follows:

[0004]

[0005] "Insecticidal Effect of Patchouli Essential Oil and Analysis of Its Active Ingredients (Zeng Qingqian et al., Journal of Plant Resources and Environment, 2006, 15(3): 21-25)" discloses that patchouli essential oil and patchouli ketone have a good control effect on the larvae of Pieris rapae and Plutella xylostella. Feeding experiments were conducted on Pieris rapae and Plutella xylostella larvae using 2.5-40 mg / mL patchouli essential oil and 1-10 mg / mL patchouli ketone, respectively, and both showed a strong antifeedant effect; the LC values ​​of patchouli essential oil and patchouli ketone on the 4th instar Pieris rapae larvae were 0. 50 The values ​​were 104.28 μg / mL and 32.20 μg / mL, respectively.

[0006] "Bioactivity of Patchouli Ketone against Spodoptera litura and Effects on Detoxification Enzymes (Zhang Jiahui et al., South China Agricultural University, Master's thesis, June 1, 2016)" discloses that patchouli ketone has stomach poison, contact toxicity, antifeedant and growth inhibitory effects on Spodoptera litura larvae, and has the prospect of being developed into an insecticide. It is also speculated that carboxylesterase may be an important site of action of patchouli ketone against Spodoptera litura.

[0007] "Chemical Synthesis of Patchouli Ketone and Its Insecticidal Activity Against Lepidoptera Pests (Huang Sihan et al., Guangzhou University of Chinese Medicine, Master's Thesis, April 1, 2014)" discloses that patchouli ketone has a strong antifeedant effect on Spodoptera litura and Spodoptera exigua, and has good toxicity against the two pests, among which the stomach toxicity activity against Spodoptera litura and Spodoptera exigua is LC 0. 50 The contact LC values ​​were 986.88 mg / L and 545.61 mg / L respectively. 50 They are 1041.42 mg / L and 519.48 mg / L.

[0008] Combining ingredients from different pesticides is a common method for developing new pesticides. In most cases, these combinations have an additive effect, with few combinations exhibiting true synergistic effects. Through extensive laboratory experiments, the inventors discovered that combining metaflumizone, spinetoram, emamectin benzoate, or chlorfenapyr with patchouli ketone exhibits a synergistic effect against Spodoptera litura.

[0009] So far, there are no reports on the combination of metaflumizone, spinetoram, emamectin benzoate or chlorantraniliprole with patchouli ketone. Summary of the Invention

[0010] The purpose of the present invention is to provide a high-efficiency, low-residue pesticide composition to solve the problem that the use of a single agent easily leads to pest resistance, resulting in reduced control efficiency; and the problem that increasing the dosage easily leads to a series of problems such as pesticide residues and environmental pollution.

[0011] To achieve the above object, the present invention provides the following technical solutions:

[0012] A high-efficiency, low-residue pesticide composition, the active ingredients of which are a combination of cyanamide, spinetoram, emamectin benzoate or chlorfenapyr, and patchouli, wherein:

[0013] The mass ratio of metaflumizone to patchouli ketone is 4-7:9-1;

[0014] The mass ratio of spinetoram to patchouli ketone is 1-40:3-1;

[0015] The mass ratio of emamectin benzoate to patchouli ketone is 1:20-8;

[0016] The mass ratio of chlorantraniliprole to patchouli ketone is 1-30:10-1.

[0017] Preferably, the mass ratio of emamectin benzoate to patchouli ketone is 1:10.

[0018] The present invention also provides a pesticide preparation, which is prepared from the high-efficiency, low-residue pesticide composition and auxiliary components accepted in pesticide science.

[0019] Compared with the prior art, the present invention has the following beneficial effects:

[0020] When metaflumizone, spinetoram, emamectin benzoate or chlorfenapyr are compounded with patchouli ketone in the pesticide composition of the present invention, a synergistic effect is exhibited within a certain mass ratio range, thereby improving the control effect on Spodoptera litura. Based on this, the application dosage of the chemical agent can be reduced, thereby avoiding, to a certain extent, the problems of pesticide residues and environmental pollution caused by high-dose application. DETAILED DESCRIPTION

[0021] The present invention can be better understood according to the following examples. The contents described in the examples are only used to illustrate the present invention, and should not and will not limit the present invention described in detail in the claims.

[0022] Example :Indoor biological activity test of patchouli ketone compound

[0023] 1. Test subjects: Spodoptera litura Fabricius, collected from rapeseed fields in the suburbs of Zhengzhou, Henan Province, were reared for multiple generations on fresh, clean rapeseed leaves in a laboratory. Healthy third-instar larvae of uniform size were selected as test subjects.

[0024] 2. Test agents

[0025] 97% cyfluthrin technical (Hebei Xingbai Agricultural Technology Co., Ltd.)

[0026] 81.2% Spinetoram technical (Corteva Agriscience Co., Ltd.)

[0027] 90% Emamectin benzoate technical (Jiangsu Fengyuan Bioengineering Co., Ltd.)

[0028] 94% chlorantraniliprole technical (Xiangshui Zhongshan Biotechnology Co., Ltd.)

[0029] 98% Patchouli ketone technical (Shanghai Yuanye Biotechnology Co., Ltd.)

[0030] The original drug was first dissolved in dimethyl sulfoxide to prepare a single-dose stock solution, and then diluted with a 0.1% Tween-80 aqueous solution. Multiple groups of ratios were set up. Each single dose and each group of mixed ratios were set to 7 gradient mass concentrations according to the equal proportion method, and 50 mL of the drug solution for each mass concentration was set aside.

[0031] 3. Test method: (Refer to "NY / T 1154.6-2006 Pesticide Indoor Bioassay Test Guidelines Insecticides Part 6: Insecticide Activity Test - Insect Immersion Method")

[0032] Test insects were immersed in the solution for 10 seconds. Excess solution was removed with filter paper and the insects were then transferred to normal rearing conditions. Each treatment was repeated four times, with 20 insects immersed in each repetition. A treatment containing only dimethyl sulfoxide and 0.1% Tween-80 served as a blank control. Mortality was observed 24 hours after treatment, and the total number of insects and the number of dead insects were recorded for each treatment. This was used to calculate the corrected mortality rate for each treatment.

[0033]

[0034] In the above formula: P is the mortality rate, in %; K is the number of dead insects; N is the total number of insects treated.

[0035]

[0036] In the above formula: P1--corrected mortality rate, unit is %; P t --Treatment mortality, unit is %; P0--blank control mortality, unit is %.

[0037] 4. Data analysis: DPS software was used to perform regression analysis on the logarithmic concentration of each treatment agent and the corrected mortality probability value of each treatment, and the LC value of each treatment agent was calculated. 50 , and calculated the co-toxicity coefficient (CTC value) of the mixture according to Sun Yunpei's method.

[0038]

[0039] In the above formula: ATI - the toxicity index of the mixture; S - the LC of the standard agent 50 , the unit is mg / L; M--LC of the test agent 50 , unit is mg / L.

[0040] TTI=TI A ×P A +TI B ×P B

[0041] In the above formula: TTI - theoretical toxicity index of mixture; TI A --Toxicity index of agent A; P A --The percentage of agent A in the mixture, expressed as percentage (%); TI B --Toxicity index of agent B; P B --The percentage of agent B in the mixture, in percentage (%).

[0042]

[0043] In the above formula: CTC - co-toxicity coefficient; ATI - measured toxicity index of mixture; TTI - theoretical toxicity index of mixture.

[0044] 5. Efficacy evaluation

[0045] The synergistic effect of the agents was evaluated based on the calculated co-toxicity coefficient (CTC). CTC ≤ 80 indicated an antagonistic effect, 80 < CTC < 120 indicated an additive effect, and CTC ≥ 120 indicated a synergistic effect. The results are shown in Tables 1-4.

[0046] Table 1 The results of toxicity test of metaflumizone and patchouli ketone against Spodoptera litura

[0047]

[0048] As can be seen from Table 1, within the mass ratio range of 4-7:9-1, the co-toxicity coefficient of metaflumizone and patchouli ketone to Spodoptera litura was greater than 120, showing a synergistic effect.

[0049] Table 2 Toxicity test results of the combination of ethyl spinosad and patchouli ketone against Spodoptera litura

[0050]

[0051] As can be seen from Table 2, within the mass ratio range of 1-40:3-1, the co-toxicity coefficient of spinetoram and patchouli ketone to Spodoptera litura was greater than 120, showing a synergistic effect.

[0052] Table 3 Toxicity test results of emamectin benzoate and patchouli ketone complex to Spodoptera litura

[0053]

[0054] As can be seen from Table 3, within the mass ratio range of 1:20-8, the co-toxicity coefficient of emamectin benzoate and patchouli ketone to Spodoptera litura was greater than 120, showing a synergistic effect; especially when the mass ratio was 1:10, the co-toxicity coefficient reached 908.02, and the synergistic effect was particularly significant.

[0055] Table 4 Toxicity test results of chlorantraniliprole and patchouli ketone complex to Spodoptera litura

[0056]

[0057] As can be seen from Table 4, within the mass ratio range of 1-30:10-1, the co-toxicity coefficient of chlorantraniliprole and patchouli ketone to Spodoptera litura was greater than 120, showing a synergistic effect.

[0058] In summary, when metaflumizone, spinetoram, emamectin benzoate or chlorfenapyr are compounded with patchouli ketone in the pesticide composition of the present invention, a synergistic effect is exhibited within a certain mass ratio range, which can improve the control effect on Spodoptera litura. Based on this, the application dosage of chemical agents can be reduced, and to a certain extent, problems such as pesticide residues and environmental pollution caused by high-dose application can be avoided.

[0059] The above is only a preferred embodiment of the present invention. Those skilled in the art can make appropriate improvements without departing from the principles of the present invention. These improvements are also within the scope of protection of the present invention.

Claims

1. A high-efficiency, low-residue pesticide composition, characterized in that: The active ingredient is prepared by compounding ethyl spinetoram and patchouli ketone, wherein the mass ratio of ethyl spinetoram to patchouli ketone is 1-40:3-1.

2. A pesticide formulation, characterized in that The pesticide preparation is prepared from the high-efficiency, low-residue pesticide composition according to claim 1 and auxiliary components accepted in pesticide science.

Images