A kit for determining liver cancer through a transmembrane glycoprotein and a method of using the same

Abstract

The present application relates to the technical field of reagent kit, specifically a reagent kit for determining liver cancer through transmembrane glycoprotein and a use method thereof, the reagent kit for determining liver cancer through transmembrane glycoprotein comprises a box body and a box cover rotatably arranged on the box body, further comprising: a partition plate installed in the box body, and a plurality of through holes are formed in the partition plate; a plurality of sets of elastic limiting mechanisms are arranged on the bottom wall of the box body, and the elastic limiting mechanisms correspond to the through holes; a plurality of sets of elastic clamping mechanisms are arranged on the upper part of the partition plate, and the elastic clamping mechanisms are connected with trigger transmission mechanisms installed on the partition plate; the elastic limiting mechanisms and the elastic clamping mechanisms can stabilize the lower end and the upper end of the reagent bottle, ensure the stability of the reagent bottle in the reagent kit during the transfer process, and effectively avoid the risk of reagent leakage caused by damage of the reagent bottle.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of reagent kits, in particular to a reagent kit for determining liver cancer through transmembrane glycoprotein and a use method thereof. BACKGROUND

[0002] Golgi transmembrane glycoprotein 73 (GP73) is an ideal serum marker for early diagnosis of liver cancer and postoperative recurrence condition evaluation, and the sensitivity and specificity are much higher than alpha-fetal protein (AFP), and if the two are combined, the diagnosis and treatment level of liver cancer will be greatly improved.

[0003] Golgi protein GP73 quantitative determination needs to use a reagent kit for convenient storage of reagents. However, in actual use, the reagent bottle is usually placed in the square of the reagent kit, although it is convenient to take, but in the process of reagent kit transportation, the reagent bottle is in a loose state in the square, which may cause strong vibration of the reagent bottle, and even cause damage to the reagent bottle, thereby increasing the risk of reagent leakage. SUMMARY

[0004] The purpose of the present application is to provide a reagent kit for determining liver cancer through transmembrane glycoprotein and a use method thereof to solve the problems raised in the background.

[0005] To achieve the above purpose, the present application provides the following technical scheme:

[0006] A reagent kit for determining liver cancer through transmembrane glycoprotein, comprising a box body and a box cover rotatably arranged on the box body, further comprising:

[0007] A partition plate is installed in the box body and divides the box body into an upper region and a lower region, and a plurality of through holes are formed in the partition plate to form a plurality of placement positions for placing reagent bottles on the partition plate;

[0008] A plurality of elastic limiting mechanisms are arranged on the bottom wall of the box body, and the elastic limiting mechanisms correspond to the through holes, and when the reagent bottle is placed in the placement position, the elastic limiting mechanism can limit the part of the reagent bottle in the through hole located in the lower region;

[0009] A plurality of elastic clamping mechanisms are arranged on the upper part of the partition plate, and the elastic clamping mechanisms are connected with a trigger transmission mechanism installed on the partition plate, the trigger transmission mechanism is triggered when the box cover is closed, and drives the elastic clamping mechanism to move, so as to make the elastic clamping mechanism execute clamping action on the part of the reagent bottle in the through hole located in the upper region.

[0010] As a further scheme of the present application, the elastic limiting mechanism comprises a plurality of driven blocks movably arranged on the bottom wall of the box body, and the driven blocks are equidistantly distributed along the circumference of the through hole; the driven blocks are connected with two groups of elastic telescopic structures mounted on the bottom wall of the box body.

[0011] As a further scheme of the present application, the driven blocks are provided with an inclined surface and an abutting surface on the side facing the central axis of the through hole; the abutting surface is perpendicular to the bottom surface of the box body; and each side of the driven block is provided with a protruding block, and the two protruding blocks are respectively connected with two groups of elastic telescopic structures.

[0012] As a further scheme of the present application, the elastic telescopic structure comprises a fixed seat mounted on the bottom wall of the box body through a vertical plate, a telescopic rod slidingly arranged in the fixed seat, and a first cylindrical spring arranged in the fixed seat.

[0013] One end of the first cylindrical spring is connected with the inner wall of the fixed seat, and the other end is connected with the leading end of the telescopic rod; and the trailing end of the telescopic rod is fixed with the protruding block.

[0014] As a further scheme of the present application, the elastic clamping mechanism comprises a facing sliding assembly mounted on the partition plate and two groups of clamping structures symmetrically arranged on the facing sliding assembly; and the facing sliding assembly is connected with the trigger transmission mechanism.

[0015] As a further scheme of the present application, the facing sliding assembly comprises two horizontal rods mounted on the partition plate and located on both sides of the through hole, and two horizontal moving plates slidingly connected with the two horizontal rods; the two horizontal moving plates are symmetric about the through hole; and two groups of clamping structures are respectively mounted on the two horizontal moving plates.

[0016] One end of each of the two horizontal moving plates is connected with a group of rolling cooperation structures mounted on the partition plate; and one group of the rolling cooperation structures is connected with the trigger transmission mechanism.

[0017] As a further scheme of the present application, the rolling cooperation structure comprises two drive wheels rotatably mounted on the partition plate and a connecting piece connecting the two drive wheels; the connecting piece is rollingly cooperated with the two drive wheels and connected with the end of the horizontal moving plate; and the rotation axes of the two drive wheels close to each other in the two groups of rolling cooperation structures are connected with a gear set.

[0018] As a further scheme of the present application, the clamping structure comprises two movable columns slidingly arranged on the horizontal moving plate, a clamping piece fixedly connected with the two movable columns on the side facing the through hole, and two third cylindrical springs respectively sleeved on the outer circumferences of the two movable columns.

[0019] The third cylindrical spring is connected to the horizontal moving plate at one end and to the clamping member at the other end.

[0020] As a further aspect of the present application, the trigger transmission mechanism comprises a vertical column slidingly arranged on the partition plate, a horizontal plate mounted on the upper end of the vertical column, and a plurality of connecting rods rotatably mounted on both sides of the horizontal plate.

[0021] The second cylindrical spring is sleeved on the outer periphery of the vertical column, and the two ends of the second cylindrical spring are connected to the partition plate and the horizontal plate respectively.

[0022] The partition plate is further provided with a guide rod, and a rack plate is slidingly arranged on the guide rod. The rack plate is rotatably connected to the end of the connecting rod away from the horizontal plate and is engaged with a gear rotatably mounted on the partition plate. The rotation shaft of the gear is connected to the rotation shaft of one of the drive wheels through a transmission belt.

[0023] A method for using the kit for determining liver cancer through transmembrane glycoprotein comprises the following steps:

[0024] Step one, align the bottom of the reagent bottle with the through hole, and press the reagent bottle downward towards the partition plate.

[0025] Step two, the bottom end of the reagent bottle contacts the elastic limiting mechanism, prompting the elastic limiting mechanism to trigger and limit the lower end of the reagent bottle.

[0026] Step three, close the box cover, so that the trigger transmission mechanism moves, and the elastic clamping mechanism is driven by the trigger transmission mechanism to move, prompting the elastic clamping mechanism to perform clamping action on the upper end of the reagent bottle.

[0027] Step four, lock the box cover and the box body, and complete the storage of the reagent bottle.

[0028] Compared with the prior art, the reagent bottle to be stored is inserted into the through hole, the bottom end of the reagent bottle moves towards the lower side of the partition plate, the elastic limiting mechanism is triggered during the process of contacting the bottom wall of the box body, the lower end of the reagent bottle is limited, then the box cover is closed, the trigger transmission mechanism is moved, the elastic clamping mechanism is moved by the trigger transmission mechanism, the upper end of the reagent bottle is clamped by the elastic clamping mechanism, therefore, through the cooperation between the mechanisms and components, the effective storage function of the reagent bottle is realized, and the lower end and the upper end of the reagent bottle are stabilized by the elastic limiting mechanism and the elastic clamping mechanism, so that the stability of the reagent bottle in the reagent box during the transfer process is ensured, and the risk of reagent leakage caused by damage of the reagent bottle is effectively avoided. BRIEF DESCRIPTION OF DRAWINGS

[0029] Figure 1 Structure diagram of one embodiment of the reagent kit for determining liver cancer by transmembrane glycoprotein;

[0030] Figure 2 Structure diagram of one embodiment of the reagent kit for determining liver cancer by transmembrane glycoprotein from another angle;

[0031] Figure 3 Partial sectional view of one embodiment of the reagent kit for determining liver cancer by transmembrane glycoprotein;

[0032] Figure 4 Structure diagram of one embodiment of the reagent kit for determining liver cancer by transmembrane glycoprotein; Figure 3 Structure diagram of one embodiment of the reagent kit for determining liver cancer by transmembrane glycoprotein;

[0033] Figure 5 Structure diagram of one embodiment of the reagent kit for determining liver cancer by transmembrane glycoprotein;

[0034] Figure 6 Structure diagram of one embodiment of the reagent kit for determining liver cancer by transmembrane glycoprotein;

[0035] Figure 7 Structure diagram of one embodiment of the reagent kit for determining liver cancer by transmembrane glycoprotein;

[0036] Figure 8 Structure diagram of one embodiment of the reagent kit for determining liver cancer by transmembrane glycoprotein;

[0037] In the figure: 1, box body; 2, box cover; 3, partition; 301, through hole; 4, horizontal plate; 5, driven block; 501, inclined surface; 502, abutting surface; 6, fixing seat; 7, telescopic rod; 8, first cylindrical spring; 9, stand; 10, second cylindrical spring; 11, pulley; 12, connecting rod; 13, guide rod; 14, rack plate; 15, gear; 16, transmission belt; 17, drive wheel; 18, connecting piece; 19, gear set; 20, cross bar; 21, horizontal moving plate; 22, movable column; 23, third cylindrical spring; 24, clamping piece; 25, protruding block; 26, vertical plate. DETAILED DESCRIPTION

[0038] The technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application. Obviously, the described embodiments are only some of the embodiments of the present application, but not all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative work fall within the scope of protection of the present application.

[0039] In addition, the elements in the present application are referred to as "fixed to" or "provided on" another element, which can be directly on another element or can have a middle element. When an element is considered to be "connected" to another element, it can be directly connected to another element or can have a middle element. The terms "vertical", "horizontal", "left", "right" and similar expressions used herein are for illustrative purposes only and do not represent the only implementation.

[0040] Please refer to Figures 1-8 In the embodiments of the present application, a kit for determining liver cancer through transmembrane glycoprotein comprises a box body 1 and a box cover 2 rotatably arranged on the box body 1, and further comprises:

[0041] A partition 3 is installed in the box body 1 and separates the box body 1 into an upper area and a lower area, and a plurality of through holes 301 are formed on the partition 3 to form a plurality of placement positions for placing reagent bottles on the partition 3;

[0042] A plurality of elastic limiting mechanisms are arranged on the bottom wall of the box body 1, and the elastic limiting mechanisms correspond to the through holes 301, and when the reagent bottles are placed in the placement positions, the elastic limiting mechanisms can limit the positions of the reagent bottles in the through holes 301 in the lower area;

[0043] The elastic clamping mechanism is provided with multiple groups at the upper part of the partition plate 3, and is connected with a trigger transmission mechanism installed on the partition plate 3. The trigger transmission mechanism is triggered when the box cover 2 is closed, and drives the elastic clamping mechanism to move, so as to make the elastic clamping mechanism perform clamping action on the part of the reagent bottle in the upper area in the through hole 301.

[0044] In use, the reagent bottle to be stored is inserted into the through hole 301, and the bottom end of the reagent bottle moves towards the lower part of the partition plate 3 until the elastic limiting mechanism is triggered during the process of contacting the bottom wall of the box body 1, and the lower end of the reagent bottle is limited. Then, the box cover 2 is closed, so that the trigger transmission mechanism moves, and the trigger transmission mechanism drives the elastic clamping mechanism to move, so that the elastic clamping mechanism clamps the upper end of the reagent bottle. Therefore, through the cooperation between the various mechanisms and components, the effective storage function of the reagent bottle is realized, and through the elastic limiting mechanism and the elastic clamping mechanism, the lower end and the upper end of the reagent bottle are stabilized, so as to ensure the stability of the reagent bottle in the reagent box during the transfer process, and avoid the adverse effects on the reagent caused by excessive vibration.

[0045] Please refer again to Figure 5 and Figure 6 , the elastic limiting mechanism comprises multiple driven blocks 5 movably arranged on the bottom wall of the box body 1, and multiple driven blocks 5 are equidistantly distributed along the circumference of the through hole 301. The driven blocks 5 are connected with two groups of elastic extension structures installed on the bottom wall of the box body 1.

[0046] The driven blocks 5 are provided with an inclined surface 501 and an abutting surface 502 on the side facing the central axis of the through hole 301. The abutting surface 502 is perpendicular to the bottom surface of the box body 1, and each side of the driven block 5 is provided with a protruding block 25. Two protruding blocks 25 are connected with two groups of elastic extension structures respectively.

[0047] The elastic extension structure comprises a fixed seat 6 installed on the bottom wall of the box body 1 through a vertical plate 26, an extension rod 7 slidingly arranged in the fixed seat 6, and a first cylindrical spring 8 arranged in the fixed seat 6.

[0048] The first cylindrical spring 8 is connected with the inner wall of the fixed seat 6 at one end, and is connected with the leading end of the extension rod 7 at the other end. The trailing end of the extension rod 7 is fixed with the protruding block 25.

[0049] In actual use, when the reagent bottle is inserted into the through hole 301, the bottom of the bottle will be in contact with the inclined surface 501, and as the staff continues to press the reagent bottle, the follower 5 will be displaced, and correspondingly, the telescopic rod 7 will gradually slide into the fixed seat 6, and the first cylindrical spring 8 will be compressed, and when the reagent bottle cannot be pressed downward any more, that is, the bottom of the reagent bottle is attached to the bottom wall of the box body 1, at this time, the abutting surface 502 abuts against the lower end of the reagent bottle, and the first cylindrical spring 8 in the compressed state provides pressure to effectively limit the lower end of the reagent bottle.

[0050] Please refer again to Figure 4 and Figure 7 , the elastic clamping mechanism includes a sliding assembly opposite to each other mounted on the partition plate 3 and two sets of clamping structures symmetrically arranged on the sliding assembly opposite to each other, and the sliding assembly opposite to each other is connected with the trigger transmission mechanism.

[0051] During the closing process of the box cover 2, the trigger transmission mechanism will be moved, and then the trigger transmission mechanism drives the sliding assembly opposite to each other to move, so that the sliding assembly opposite to each other drives the two sets of clamping structures to move close to each other, and after the clamping structure contacts with the reagent bottle, it will start to store elastic potential energy to complete the flexible clamping of the upper end of the reagent bottle.

[0052] The sliding assembly opposite to each other includes two horizontal rods 20 mounted on the partition plate 3 and located on both sides of the through hole 301, and two horizontal moving plates 21 in sliding connection with the two horizontal rods 20, and the two horizontal moving plates 21 are symmetrical about the through hole 301, and two sets of clamping structures are mounted on the two horizontal moving plates 21;

[0053] Among them, one end of each of the two horizontal moving plates 21 is connected with a set of rolling fitting structure mounted on the partition plate 3, and one set of the rolling fitting structure is connected with the trigger transmission mechanism.

[0054] The rolling fitting structure includes two drive wheels 17 rotatably mounted on the partition plate 3 and a connecting piece 18 connecting the two drive wheels 17, and the connecting piece 18 is in rolling fit with the two drive wheels 17 and is connected with the end of the horizontal moving plate 21, and the rotation axes of the two drive wheels 17 close to each other in the two sets of rolling fitting structures are connected with a gear set 19.

[0055] In detail, the gear set 19 includes a first gear and a second gear coaxially mounted with the two drive wheels 17 close to each other, respectively, and the first gear is in meshing with the second gear.

[0056] When the trigger transmission mechanism moves, one of the driving wheels 17 will be rotated, and under the direction of the gear set 19, the connecting piece 18 will drive the sliding plates 21 to gradually slide on the cross rod 20 towards the through hole 301, and correspondingly, the two sliding plates 21 will move closer to each other, and the two sets of clamping structures will move with the two sliding plates 21 to clamp the upper end of the reagent bottle in the through hole 301.

[0057] The clamping structure includes two movable columns 22 slidingly arranged on the sliding plates 21, a clamping piece 24 fixedly connected to one end of the two movable columns 22 towards the through hole 301, and two third column springs 23 respectively sleeved on the outer periphery of the two movable columns 22.

[0058] The clamping piece 24 is arranged in an arc shape for clamping the reagent bottle, one end of the third column spring 23 is connected to the sliding plate 21, and the other end is connected to the clamping piece 24.

[0059] During the movement of the two sliding plates 21 towards each other, the two clamping pieces 24 also move towards each other. After the clamping piece 24 contacts the outer wall of the reagent bottle, and the sliding plate 21 continues to move, the clamping piece 24 remains stationary, causing the movable column 22 to slide relative to the sliding plate 21, the distance between the sliding plate 21 and the clamping piece 24 gradually decreases, and the third column spring 23 is gradually compressed. After the box cover 2 is closed and locked, the two clamping pieces 24 maintain the clamping state of the upper end of the reagent bottle. The arrangement of the third column spring 23 realizes the flexible clamping feature of the clamping piece 24 on the reagent bottle, effectively preventing the deformation of the reagent bottle caused by rigid contact.

[0060] Please refer again to Figure 8 The trigger transmission mechanism includes a stand 9 slidingly arranged on the partition plate 3, a cross plate 4 installed on the upper end of the stand 9, and a plurality of connecting rods 12 rotatably installed on both sides of the cross plate 4.

[0061] The outer periphery of the stand 9 is sleeved with a second column spring 10, the two ends of the second column spring 10 are respectively connected to the partition plate 3 and the cross plate 4, and a pulley 11 is rotatably installed on the upper part of the cross plate 4.

[0062] The partition plate 3 is also provided with a guide rod 13, a rack plate 14 is slidingly arranged on the guide rod 13, the rack plate 14 is rotatably connected to the end of the connecting rod 12 away from the cross plate 4, and is engaged with a gear 15 rotatably installed on the partition plate 3, and the rotating shaft of the gear 15 is connected to the rotating shaft of one of the driving wheels 17 through a transmission belt 16.

[0063] The box cover 2 contacts the pulley 11 during closing, and as the box cover 2 continues to close, the pulley 11 rolls on the inner wall of the box cover 2, causing the horizontal plate 4 and the stand 9 to move downward, the second cylindrical spring 10 is compressed, at the same time, the horizontal plate 4 pushes the rack plate 14 to slide on the guide rod 13 through the connecting rod 12, causing the gear 15 to rotate, so that the rotating shaft of the gear 15 can drive the driving wheel 17 to rotate, so that the two clamping members 24 move close to each other, and the reagent bottle is clamped.

[0064] As another embodiment of the present application, a method for using the kit for determining liver cancer through transmembrane glycoprotein is also provided, comprising the following steps:

[0065] Step one, align the bottom of the reagent bottle with the through hole 301, and press the reagent bottle downward to the lower part of the partition plate 3;

[0066] Step two, the bottom end of the reagent bottle contacts the elastic limiting mechanism, causing the elastic limiting mechanism to trigger and limit the lower end of the reagent bottle;

[0067] Step three, close the box cover 2, so that the trigger transmission mechanism moves, and the elastic clamping mechanism moves driven by the trigger transmission mechanism, causing the elastic clamping mechanism to clamp the upper end of the reagent bottle;

[0068] Step four, lock the box cover 2 and the box body 1, and complete the storage of the reagent.

[0069] It is obvious to those skilled in the art that the present application is not limited to the details of the above exemplary embodiments, and can be implemented in other specific forms without departing from the spirit or essential characteristics of the present application. Therefore, the embodiments should be regarded as exemplary and non-limiting, and the scope of the present application is defined by the appended claims rather than the above description, and all changes falling within the meaning and scope of the equivalent elements of the claims are intended to be included in the present application. Any reference signs in the claims should not be regarded as limiting the claims involved.

[0070] In addition, it should be understood that although the present specification is described in terms of embodiments, not every embodiment contains only one independent technical solution, and the description of the specification is only for the sake of clarity, and those skilled in the art should consider the specification as a whole, and the technical solutions in each embodiment can be combined appropriately to form other embodiments that those skilled in the art can understand.

Claims

1. A kit for determining liver cancer by a transmembrane glycoprotein, characterized by, The utility model relates to a reagent bottle placing box, including box body (1) and the box cover (2) of rotation is established in box body (1), still including: Partition (3) is installed in box body (1) and will box body (1) is divided into upper area and lower area, and a plurality of through -holes (301) are seted up on partition (3) to make a plurality of placing positions for placing reagent bottle on partition (3) are formed, Elastic limit mechanism is equipped with multiple sets on the bottom wall of box body (1), and elastic limit mechanism corresponds with through -hole (301), when reagent bottle is placed in placing position, elastic limit mechanism can limit the part of reagent bottle in through -hole (301) in lower area, Elastic clamping mechanism is equipped with multiple sets on the upper portion of partition (3), and elastic clamping mechanism is connected with trigger transmission mechanism installed on partition (3), trigger transmission mechanism triggers when box cover (2) is closed and drives elastic clamping mechanism to move to make elastic clamping mechanism execute clamping action to the part of reagent bottle in through -hole (301) in upper area, Elastic clamping mechanism includes facing sliding assembly installed on partition (3) and two groups of clamping structure symmetrically arranged on facing sliding assembly, and facing sliding assembly is connected with trigger transmission mechanism, Facing sliding assembly includes two horizontal rods (20) installed on partition (3) and located on both sides of through -hole (301), two horizontal moving plates (21) are slidingly connected with two horizontal rods (20), and two horizontal moving plates (21) are symmetrical about through -hole (301), and two groups of clamping structure are installed on two horizontal moving plates (21) respectively, Wherein, one end of two horizontal moving plates (21) is connected with a group of rolling cooperation structure installed on partition (3), and one group of rolling cooperation structure is connected with trigger transmission mechanism, Rolling cooperation structure includes two drive wheels (17) rotationally installed on partition (3) and connecting piece (18) connected with two drive wheels (17), and connecting piece (18) is rollingly cooperated with two drive wheels (17) and is connected with the end of horizontal moving plate (21), and gear set (19) is connected between the rotation axes of two drive wheels (17) close to each other in two groups of rolling cooperation structure; Trigger transmission mechanism includes vertical column (9) slidingly arranged on partition (3), horizontal plate (4) installed on the upper end of vertical column (9) and a plurality of connecting rods (12) rotationally installed on both sides of horizontal plate (4); Wherein, the outer periphery of vertical column (9) is sleeved with second columnar spring (10), and two ends of second columnar spring (10) are connected with partition (3) and horizontal plate (4) respectively, and the upper portion of horizontal plate (4) is also rotationally installed with pulley (11); A guide rod (13) is further installed on the partition plate (3), a rack plate (14) is slidably arranged on the guide rod (13), the rack plate (14) is rotatably connected to one end of the connecting rod (12) away from the horizontal plate (4) and is engaged with a gear (15) rotatably installed on the partition plate (3), and the rotation shaft of the gear (15) is connected to the rotation shaft of one of the driving wheels (17) through a transmission belt (16).

2. The kit for determining liver cancer through a transmembrane glycoprotein according to claim 1, wherein, The elastic limiting mechanism comprises a plurality of driven blocks (5) movably arranged on the bottom wall of the box body (1), and the plurality of driven blocks (5) are equidistantly distributed along the circumference of the through hole (301). The driven blocks (5) are connected with two groups of elastic extension structures installed on the bottom wall of the box body (1).

3. The kit for determining liver cancer through a transmembrane glycoprotein according to claim 2, wherein, The side of the driven block (5) facing the central axis of the through hole (301) is provided with an inclined surface (501) and an abutting surface (502). The abutting surface (502) is perpendicular to the bottom surface of the box body (1), and each side of the driven block (5) is provided with a protruding block (25). The two protruding blocks (25) are respectively connected with the two groups of elastic extension structures.

4. The kit for determining liver cancer through a transmembrane glycoprotein according to claim 3, wherein, The elastic extension structure comprises a fixed seat (6) installed on the bottom wall of the box body (1) through a vertical plate (26), a telescopic rod (7) slidably arranged in the fixed seat (6), and a first cylindrical spring (8) arranged in the fixed seat (6). One end of the first cylindrical spring (8) is connected to the inner wall of the fixed seat (6), and the other end is connected to the leading end of the telescopic rod (7). The trailing end of the telescopic rod (7) is fixed to the protruding block (25).

5. The kit for determining liver cancer through a transmembrane glycoprotein according to claim 1, wherein the antibody is an antibody recognizing a glycoprotein having a molecular weight of 70 kDa to 80 kDa. The clamping structure comprises two movable columns (22) slidably arranged on the horizontal moving plate (21), a clamping piece (24) fixedly connected to one end of the two movable columns (22) facing the through hole (301), and two third cylindrical springs (23) respectively sleeved on the outer periphery of the two movable columns (22). The clamping piece (24) is arranged in an arc shape and is used for clamping the reagent bottle. One end of the third cylindrical spring (23) is connected to the horizontal moving plate (21), and the other end is connected to the clamping piece (24).

6. A method for using the kit for determining liver cancer by transmembrane glycoprotein according to claim 1, characterized in that, The steps include: Step one: align the bottom of the reagent bottle with the through hole (301), and press the reagent bottle downwardly to the partition plate (3); Step two: the bottom end of the reagent bottle contacts the elastic limiting mechanism, which triggers the elastic limiting mechanism to limit the lower end of the reagent bottle; Step three: close the box cover (2) to make the trigger transmission mechanism move, and drive the elastic clamping mechanism to move, so as to clamp the upper end of the reagent bottle by the elastic clamping mechanism; Step four: lock the box cover (2) and the box body (1) to complete the storage of the reagent.

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