Immunoassay microfluidic chip
By designing a continuous S-shaped structure for the antibody reconstitution channel and setting up a turbulence column in the microfluidic chip, the problem of poor reconstitution effect of fluorescent antibodies was solved, and higher antibody reconstitution rate and detection accuracy were achieved.
CN116840459BActive Publication Date: 2026-07-07BOE TECHNOLOGY GROUP CO LTD
Patent Information
- Application Number
- CN202210303520.4
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2022-03-24
- Publication Date
- 2026-07-07
- Estimated Expiration
- 2042-03-24
AI Technical Summary
Technical Problem
Existing microfluidic chips using the double-antibody sandwich method for detection exhibit poor reconstitution of fluorescent antibodies, affecting the accuracy of the detection results.
Method used
The antibody reconstitution channel is designed as a continuous S-shaped structure, with alternating first and second annular channels. The channels open in opposite directions, and a turbulence column is placed inside the channel to improve the reconstitution rate of the fluorescent antibody.
Benefits of technology
By designing a secondary flow vortex and a turbulence column, the binding rate of fluorescent antibodies to antigens in the sample was significantly improved, thereby enhancing the accuracy of the detection results.
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Figure CN116840459B_ABST
Abstract
The application relates to an immune analysis micro-fluidic chip, which comprises a chip body, the chip body defining an antibody reconstitution flow channel, a fixing area and a waste pool in sequence, the antibody reconstitution flow channel comprising first and second annular flow channels arranged alternately, the first and second annular flow channels being opposite in opening direction, the first annular flow channel having a circumferential angle of alpha between the two ends along the extension direction, the second annular flow channel having a circumferential angle of beta between the two ends along the extension direction, and alpha and beta satisfying 180 DEG < alpha <= 270 DEG and 180 DEG < beta <= 270 DEG. According to the immune analysis micro-fluidic chip, the reconstitution rate of fluorescent antibodies can be improved, and the combination rate of the fluorescent antibodies and antigens in samples can be improved.
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Citation Information
Patent Citations
Single-channel fluorescence immunoassay micro-fluidic chip
CN110470604A
KR20210108754A