A composition with soothing sensitive skin function and its preparation method and application

Abstract

The present application relates to the technical field of skin care products, and discloses a composition with the effect of soothing sensitive skin, which is composed of the following components in mass percentage: plant extract 10-12%, skin repair factor 2-3%, jasmine hydrolat 10%, humectant 6-7%, and the balance being water. The composition with the effect of soothing sensitive skin has significant soothing effect, which is close to the soothing effect of common hormone ointments on the market. The composition can significantly reduce inflammatory factors, has good anti-inflammatory effect, simultaneously repairs damaged skin, enhances the moisturizing function, and enhances the skin barrier through the moisturizing and repairing effect. The composition has the effect of resisting anaphylaxis by reducing the content of trypsin and inhibiting the allergic reaction of mast cells. Human patch test and fish embryo development toxicity test prove that the composition with the effect of soothing sensitive skin has no skin adverse reactions and no development toxicity, is mild, safe and non-irritating, and is suitable for preparing skin care products for adults and infants.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of skin care products, in particular to a composition with the effect of soothing sensitive skin and a preparation method and application thereof. BACKGROUND

[0002] According to the 2023 Mintel consumer research data, about 41% of people in China belong to sensitive skin or allergic skin, which has increased a lot compared with 35% in 2021. Skin sensitivity is congenital and acquired, but the symptoms are generally similar, the skin is easy to blush, the skin is relatively weak, itching, etc., the disease incidence is high and difficult to diagnose and treat, which affects the quality of life of patients; eczema, atopic dermatitis and other allergic dermatitis are common.

[0003] Medical research found that the cause of sensitive skin is closely related to the body's allergic reaction mechanism. Allergic reactions produce an antibody called immunoglobulin E (IgE). IgE binds to a type of white blood cell called basophils in the bloodstream and similar types of cells called mast cells in tissues. The basophils and mast cells with IgE on the surface release some substances (such as histamine, prostacyclin and leukotrienes, etc.) to cause swelling or inflammation of the surrounding tissues. According to this finding, many Western doctors will prescribe steroid ointments for patients. Steroid ointments (hydrocortisone, etc.) can effectively reduce inflammation and regulate the immune system, but long-term use of steroid drugs can cause adverse side effects, such as thinning of the skin and skin damage.

[0004] At present, merchants on the market are also increasingly concerned about a series of problems of sensitive skin, and have launched a series of soothing products. The current soothing skin care products are mainly for moisturizing, and there are not many soothing skin care products. Western medicine recommends moisturizing and hormone ointment for allergic skin, but long-term use of hormone ointment can be addictive, and is prone to recurrence or stronger hormone strength. Many skin care products that claim to soothe are tested separately, and there is no comparison with hormone drugs to understand whether the product has a similar soothing effect as hormone drugs. Few hormone-free soothing skin care products can compare with steroid ointment, and the choice of allergic skin consumers is very limited. SUMMARY

[0005] In view of the above shortcomings, the present application provides a composition with the effect of soothing sensitive skin, which has anti-inflammatory and anti-allergic effects and also has the effects of repairing and moisturizing the skin, is safe and reliable; the specific technical scheme is as follows:

[0006] A composition with the effect of soothing sensitive skin, consisting of the following mass percentage components: plant extract 10-12%, skin repair factor 2-4%, jasmine hydrol 10%, moisturizing agent 6-7%, and the balance being water.

[0007] Preferably, in the composition having the effect of soothing sensitive skin, the plant extract solution comprises 18-22 wt.% plant extracts, the plant extracts are Sophora flavescens extract, Semen Vaccariae extract and Dictamnus dasycarpus extract, the weight ratio of Sophora flavescens extract, Semen Vaccariae extract and Dictamnus dasycarpus extract is 3-5:3-5:2-2.8, and the amount of Sophora flavescens extract is the same as that of Semen Vaccariae extract.

[0008] Preferably, in the composition having the effect of soothing sensitive skin, the weight ratio of Sophora flavescens extract, Semen Vaccariae extract and Dictamnus dasycarpus extract is 4:4:2.5.

[0009] Preferably, in the composition having the effect of soothing sensitive skin, the skin repair factor is composed of the following components: β-glucan, nicotinamide, edelfosine, squalane, sodium hyaluronate and allantoin, and the mass percentage of each component in the composition is: β-glucan 0.2-0.4%, nicotinamide 1.3-2.5%, edelfosine 0.08-0.12%, squalane 0.3-0.8%, sodium hyaluronate 0.04-0.06% and allantoin 0.08-0.12%.

[0010] Preferably, in the composition having the effect of soothing sensitive skin, the mass percentage of each component of the skin repair factor is: β-glucan 0.3%, nicotinamide 2%, edelfosine 0.1%, squalane 0.5%, sodium hyaluronate 0.05% and allantoin 0.1%.

[0011] Preferably, in the composition having the effect of soothing sensitive skin, the humectant is composed of the following components: glycerol, 1,2-hexanediol, caprylhydroxamic acid, capryloyl glycine and 1,3-propanediol.

[0012] A preparation method of the composition having the effect of soothing sensitive skin, comprising the following steps:

[0013] (1) mixing allantoin, edelfosine, sodium hyaluronate, β-glucan, glycerol and water to obtain phase A;

[0014] (2) uniformly mixing 1,2-hexanediol, caprylhydroxamic acid, capryloyl glycine, 1,3-propanediol, nicotinamide and squalane, and then mixing with the plant extract solution and jasmine hydrol to obtain phase B;

[0015] (3) weighing phase A and adding it into a stirring pot, heating to 80-85°C, setting the stirring speed to 1200-1800 RPM for 20-40 minutes, and homogenizing at 1500-2100 RPM for 5-7 minutes to completely dissolve;

[0016] (4) cooling to below 45℃, taking B phase into the stirring pot, stirring at 1500-2100 RPM for 10-15 minutes, mixing evenly, to obtain the composition with soothing sensitive skin function.

[0017] The composition with soothing sensitive skin function described above is applied in the preparation of skin care products.

[0018] Preferably, in the application described above, the skin care product is essence, cream, emulsion or mask.

[0019] Compared with the prior art, the composition with soothing sensitive skin function of the present application has the following advantages:

[0020] 1. The composition with soothing sensitive skin function of the present application combines plant extracts, skin repair factors and other components, and each component synergizes with each other, having significant soothing effect, which is close to the soothing effect of common hormone ointment in the market through test verification; can significantly reduce inflammatory factors, has good anti-inflammatory effect, and also repairs damaged skin, enhances moisturizing function, and enhances skin barrier through moisturizing and repairing effect; has anti-allergy effect by reducing trypsin content to inhibit the allergic reaction of mast cells. Human patch test and fish embryo development toxicity test prove that the composition with soothing sensitive skin function of the present application has no skin adverse reactions and no development toxicity, is mild, safe and non-irritating, and is suitable for preparing skin care products for adults and infants.

[0021] 2. The composition with soothing sensitive skin function of the present application achieves similar soothing effect as hormone ointment (hydrocortisone acetate 1%) without adding sugar corticosteroids, avoids the chance of addiction to long-term use of hormone ointment, and provides a safer and more effective choice for long-term users of hormone ointment, while matching with multiple high-efficiency skin care ingredients to promote the repair of damaged skin, thereby improving the state of allergic skin without hormones.

[0022] 3. The composition with soothing sensitive skin function of the present application has the advantages of easy acquisition of raw materials, simple preparation method, green safety, high acceptance and safety of allergic people, good application prospect and market prospect. BRIEF DESCRIPTION OF DRAWINGS

[0023] In order to more clearly illustrate the technical solutions of the embodiments of the present application, the following will briefly introduce the drawings needed to be used in the embodiment description. Obviously, the drawings described below are some embodiments of the present application, and those skilled in the art can also obtain other drawings according to these drawings without creative labor.

[0024] Figure 1 The soothing typical diagram in test example 1 of the present application, the part in the dotted line in the diagram is the neutrophil quantity quantitative area.

[0025] Figure 2 A column chart of relative expression amount of IL-6 gene in the test example 2 of the present application.

[0026] Figure 3 A typical graph of scratch test in the test example 3 of the present application.

[0027] Figure 4 A column chart of trypsin expression level in the test example 4 of the present application.

[0028] Figure 5 A difference analysis chart of skin horny layer water content in the test example 5 of the present application, compared with the reference value (D0), ***p < 0.001.

[0029] Figure 6 A typical graph of baby cosmetic safety in the test example 6 of the present application, H is heart, J is lower jaw; In is intestinal tract, L is liver; E is eye; B is brain; M is muscle. DETAILED DESCRIPTION

[0030] The specific embodiments of the present application are described in detail below, but it should be understood that the scope of protection of the present application is not limited by the specific embodiments. Unless otherwise defined, all professional terms used in the following are the same as those commonly understood by those skilled in the art. The professional terms used in this paper are only for the purpose of describing specific embodiments and are not intended to limit the scope of protection of the present application. Unless otherwise specified, various raw materials, reagents, instruments and equipment used in the present application can be purchased from the market or can be prepared by existing methods.

[0031] Raw material sources:

[0032] Ku Shen extract, Bai Xianpi extract, and Gekko extract were purchased from Changsha Nongjian Technology Co., Ltd., each containing 20 wt.% plant extract (Ku Shen extract, Bai Xianpi extract, and Gekko extract), 0.3 wt.% p-hydroxyacetophenone, and the rest water. Jasmine hydrolate (100% low-temperature extraction hydrolate) was purchased from Guangxi Hengzhou Zhengda Fragrance Co., Ltd.

[0033] Example 1

[0034] A composition with soothing sensitive skin function, consisting of the following mass percentage components: plant extract 10.5%, beta-glucan 0.3%, nicotinamide 2%, ectoine 0.1%, squalane 0.5%, sodium hyaluronate 0.05%, allantoin 0.1%, jasmine hydrol 10%, glycerol 6%, 1,2-hexanediol 0.24%, capryloyl hydroxamic acid 0.04%, capryloyl glycine 0.032% and 1,3-propanediol 0.488%, the balance being water, water being 69.65%; the plant extract includes Sophora flavescens extract, Dictamnus dasycarpus extract and Semen Vaccariae extract, the weight ratio of Sophora flavescens extract, Semen Vaccariae extract and Dictamnus dasycarpus extract being 4:4:2.5.

[0035] A preparation method of a composition with soothing sensitive skin function, comprising the following steps:

[0036] (1) mixing allantoin, ectoine, sodium hyaluronate, beta-glucan, glycerol and water to obtain phase A;

[0037] (2) mixing Sophora flavescens extract, Dictamnus dasycarpus extract and Semen Vaccariae extract to obtain plant extract; uniformly mixing 1,2-hexanediol, capryloyl hydroxamic acid, capryloyl glycine, 1,3-propanediol, nicotinamide and squalane, and then mixing with the plant extract and jasmine hydrol to obtain phase B;

[0038] (3) weighing phase A and adding it into a stirring pot, heating to 85°C, setting the stirring speed to 1500 RPM and stirring for 30 minutes, homogenizing at 1800 RPM for 6 minutes, and completely dissolving;

[0039] (4) cooling to below 45°C, weighing phase B and adding it into the stirring pot, stirring at 1500 RPM for 10 minutes to uniformly mix, and obtaining the composition with soothing sensitive skin function.

[0040] The composition with soothing sensitive skin function is used for preparing soothing essence water, and the preparation method of the soothing essence water is the same as that of the composition with soothing sensitive skin function.

[0041] Example 2

[0042] A composition with soothing sensitive skin function, consisting of the following mass percentage components: plant extract 12%, beta-glucan 0.4%, nicotinamide 1.5%, ectoine 0.12%, squalane 0.7%, sodium hyaluronate 0.06%, allantoin 0.12%, jasmine hydrol 10%, glycerol 6%, 1,2-hexanediol 0.24%, capryloyl hydroxamic acid 0.04%, capryloyl glycine 0.032% and 1,3-propanediol 0.488%, the balance being water; the plant extract includes Sophora flavescens extract, Dictamnus dasycarpus extract and Semen Vaccariae extract, the weight ratio of Sophora flavescens extract, Semen Vaccariae extract and Dictamnus dasycarpus extract being 3.5:4:2.

[0043] The composition with soothing sensitive skin function of the present example is prepared by the same method as example 1.

[0044] Example 3

[0045] A composition with soothing sensitive skin function, consisting of the following components in mass percentage: plant extract 11%, β-glucan 0.3%, nicotinamide 2.2%, ectoine 0.10%, squalane 0.5%, sodium hyaluronate 0.04%, allantoin 0.08%, jasmine hydrolate 10%, glycerin 5.5%, 1,2-hexanediol 0.24%, capryloyl hydroxamic acid 0.04%, capryloyl glycine 0.032% and 1,3-propanediol 0.488%, the rest being water; the plant extract includes Sophora flavescens extract, Dictamnus dasycarpus extract and Semen Vaccariae extract, the weight ratio of Sophora flavescens extract, Dictamnus dasycarpus extract and Semen Vaccariae extract is 3:3:2.

[0046] The composition with soothing sensitive skin function of the present example is prepared by the same method as example 1.

[0047] Comparative Example 1

[0048] A composition with soothing sensitive skin function, consisting of the following components in mass percentage: plant extract 10.5%, β-glucan 0.3%, nicotinamide 2%, ectoine 0.1%, squalane 0.5%, sodium hyaluronate 0.05%, allantoin 0.1%, rose hydrolate (100% low-temperature extracted white rose hydrolate) 10%, glycerin 6%, 1,2-hexanediol 0.24%, capryloyl hydroxamic acid 0.04%, capryloyl glycine 0.032% and 1,3-propanediol 0.488%, the rest being water, water is 69.65%; the plant extract includes Sophora flavescens extract, Dictamnus dasycarpus extract and Semen Vaccariae extract, the weight ratio of Sophora flavescens extract, Dictamnus dasycarpus extract and Semen Vaccariae extract is 4:4:2.5. The preparation method is the same as example 1.

[0049] Comparative Example 2

[0050] A composition with soothing sensitive skin function, consisting of the following components in mass percentage: plant extract 10.5%, β-glucan 2.3%, ectoine 0.1%, squalane 0.5%, sodium hyaluronate 0.05%, allantoin 0.1%, rose hydrolate 10%, glycerin 6%, 1,2-hexanediol 0.24%, capryloyl hydroxamic acid 0.04%, capryloyl glycine 0.032% and 1,3-propanediol 0.488%, the rest being water, water is 69.65%; the plant extract includes Sophora flavescens extract, Dictamnus dasycarpus extract and Semen Vaccariae extract, the weight ratio of Sophora flavescens extract, Dictamnus dasycarpus extract and Semen Vaccariae extract is 4:4:2.5. The preparation method is the same as example 1.

[0051] The composition with soothing sensitive skin function of the present comparative example was used to prepare soothing essence water, and the preparation method was the same as that of Example 1.

[0052] Test Example 1 Zebrafish Soothing Function Test

[0053] The soothing essence water prepared in Example 1 was compared with the steroid hydrocortisone cream 1% (Hubei Kedian Pharmaceutical Co., Ltd.) for testing, and the zebrafish larva neutrophil inhibition rate method was used for testing.

[0054] Test object: transgenic neutrophil green fluorescent zebrafish (MPX), fish age: 2 days after fertilization (2dpf), experimental sample amount of each group: 15;

[0055] Test method:

[0056] Randomly selected zebrafish in a 6-well plate, 15 in each well; SLS was given in water to establish a zebrafish skin inflammation model. The sample was given in water, and a normal control group and a model control group were set up, and the volume of each well was 3 mL. Incubate at 28°C in the dark for 18h. Randomly select 10 zebrafish from each experimental group and place them under a fluorescence microscope for photography. Use advanced image processing software to analyze and collect data, analyze the number of neutrophils in the skin of zebrafish (N), and calculate the soothing effect of the sample according to the following formula to determine whether it has soothing effect. The test results are shown in Table 1 and Table 2. Figure 1 and Table 1.

[0057]

[0058] As shown in Table 1 and Table 2, Figure 1 The number of zebrafish neutrophils of the soothing essence water of the present application was significantly reduced compared with the model control group, indicating that the composition with soothing sensitive skin function of the present application and the soothing essence water had significant soothing effect and could inhibit allergic reactions, and the efficacy was comparable to that of the steroid cream hydrocortisone cream 1%.

[0059] Table 1 Soothing effect of the soothing essence water of the present application

[0060]

[0061] Test Example 2 Anti-inflammatory Efficacy Test

[0062] Test sample: soothing essence water of Example 1

[0063] Test object: human immortalized keratinocytes (HaCaT), cell passage number: at least 2 passages, experimental cell amount of each group: 6x10 5 cells / well;

[0064] The test method includes the following steps:

[0065] (1) Cell inoculation into 6-well plates (6 x 10 5 cells / well), 1 mL of liquid per well, 37°C, 5% CO2 incubation for 24 h.

[0066] (2) Modeling: after incubation, expose the sample group and the model control group to an ultraviolet lamp (80 mJ / cm 2 ).

[0067] (3) Drug administration: after modeling, replace the normal control group and the model control group with fresh complete culture medium, and add complete culture medium containing the sample to the sample group wells, 1 mL per well, 37°C, 5% CO2 incubation for 24 h.

[0068] (4) Extract total RNA from each experimental group, synthesize cDNA, and use q-PCR to detect the gene expression of β-actin and the target gene.

[0069] (5) Use β-actin as the internal reference for gene expression to calculate the RNA relative expression amount of the target gene.

[0070] RNA relative expression amount = 2 ΔΔC(t)

[0071] ΔΔC(t) = ΔC(t) 模型对照组 - ΔC(t) 样品组

[0072] ΔC(T) = C(t) 目的基因 - C(t) β-actin

[0073] The test results are shown in Table 2 and Figure 2 According to the results, the IL-6 gene relative expression amount of the sample group treated with the soothing serum of the present application is significantly reduced compared with the model control group. IL-6 is a main inflammatory factor for regulating inflammatory response, indicating that the soothing serum has anti-inflammatory efficacy.

[0074] Table 2 Anti-inflammatory efficacy of the soothing serum of the present application

[0075]

[0076] Test Example 3 Repair Efficacy Test

[0077] Test sample: the soothing serum of Example 1, the soothing serum of Comparative Example 1, and the soothing serum of Comparative Example 2;

[0078] Test object: human immortalized keratinocytes (HaCaT), cell passage number: at least 2 passages, and experimental cell amount per group: 6 x 10 5 cells / well;

[0079] The scratch test model is established by using human immortalized keratinocytes HaCaT to detect whether the tested substance has the effects of promoting proliferation and repairing scratch on the cells, so as to evaluate whether the sample has the repairing effect.

[0080] The test method comprises the following steps:

[0081] (1) HaCaT cells are inoculated in a 6-well plate at 6x10 5 per well, and are cultured at 37 DEG C in 5% CO2 for 24±2h.

[0082] (2) Scratch, PBS (gentle) washing twice, normal control well adding fresh culture medium, sample well adding fresh culture medium containing corresponding concentration of sample, 37 DEG C, 5% CO2 culture for 24±2h, respectively at 0h and 24h of administration, taking pictures, and recording the scratch area.

[0083] (3) Image J analyzes the scratch area of each group, and calculates the healing area (Y).

[0084]

[0085] The test results are shown in Table 3, and the treatment effect diagram of the soothing essence water of Example 1 is shown in Figure 3 According to the results, the scratch area after the soothing essence water of the application is treated for 24h is obviously smaller than that of the normal control group, which shows that the soothing essence water of the application has the repairing effect. Compared with rose hydrolate (Comparative Example 1), the combination of jasmine hydrolate of the application has better repairing effect; compared with Comparative Example 2, the addition of nicotinamide and the interaction of β-glucan, ectoine, squalane, sodium hyaluronate, allantoin and other components in the application can play a better repairing effect.

[0086] Table 3 Repairing effect of the soothing essence water of the application

[0087]

[0088] Test Example 4 Zebrafish Anti-allergy Efficacy Test

[0089] Test sample: soothing essence water of Example 1

[0090] Test object: wild type AB strain zebrafish, fish age: 2 days after fertilization (2dpf), and the number of experimental samples in each group: 10;

[0091] Tryptase is a pre-synthesized neutral protease in mast cells, and is an important index for testing allergic reactions in clinic. Zebrafish also induces mast cells to secrete tryptase when allergic reactions occur, and the latter is complexed with BAPNA substrate, which has a maximum absorption peak at 405 nm. The relative absorbance value (OD405 value) of the whole zebrafish tryptase expression level is detected by an enzyme marker, and the expression level of tryptase is used to evaluate whether the sample has anti-allergic effect.

[0092] The test method comprises the following steps:

[0093] (1) Randomly select zebrafish in a 12-well plate, 10 zebrafish per well;

[0094] (2) Water-soluble administration of 2, 4-dinitrochlorobenzene (DNCB) to establish an allergic model of zebrafish;

[0095] (3) Water-soluble administration of the sample to the sample group, and setting up a normal control group and a model control group, with a volume of 1 mL per well; three biological repeats;

[0096] (4) Incubate each experimental group and BAPNA at 28℃ under dark conditions for 24 hours;

[0097] (5) Detect the OD value (tryptase content) of each experimental group of zebrafish at 405 nm by an enzyme marker, calculate the tryptase expression level (T) of zebrafish, and calculate and judge whether the sample has anti-allergic effect according to the formula.

[0098]

[0099] The test results are shown in Table 4 and Figure 4 According to the results, the tryptase expression level of the soothing essence water sample group of the present application is compared with that of the model control group, which shows that the soothing essence water of the present application has anti-allergic effect.

[0100] Table 4 Anti-allergic effect of the soothing essence water of the present application

[0101] Sample name Detection concentration (%) Anti-allergic efficacy (%) P value Detection result Shu Min essence water 0.5 93 <0.05 Significant

[0102] Test Example 5 Moisturizing Effect Test

[0103] Test sample: soothing essence water of Example 1

[0104] Test object: 25 volunteers aged 37 to 52 years old, who have not used hormone drugs and immunosuppressants in the past month;

[0105] Test instrument: CM825 (CM825, Courage + Khazaka, Germany)

[0106] The test method comprises the following steps:

[0107] (1) Before starting the test, the subject needs to clean the inner side of the forearm of both hands uniformly, and the cleaning method is to wipe clean with dry facial tissues;

[0108] (2) The inner side of the forearm of the subject should be marked with a measurement area, and the test area should be at least 3cm*3cm, and multiple areas can be marked on the same arm at the same time, and each area is separated by at least 1cm;

[0109] (3) Before the formal test, at least 20 minutes should be spent in a standard room, and water and beverages should not be drunk. The forearm is exposed and placed in a test state, and remains relaxed;

[0110] (4) The product application area and the blank control area should be randomly distributed in the marked area of the left and right arms, and it is ensured that the positions of all products and blank areas are balanced in statistics. The test sample is applied in a single application with a dosage of (2.0±0.1) mg / cm 2 , and the sample is evenly applied to the test area using a latex finger cot, and the actual application amount is recorded.

[0111] (5) After adjusting the instrument according to the use instruction of the capacitance method skin moisture tester, the product area and the control area are measured, and each area is measured at least 3 times in parallel. The initial value of each test area (D0 before sample use) is measured, and then the skin moisture content of the test area and the control area is measured at the set time (D0T4h, D0T8h). The skin keratin layer moisture content is measured by CM825; the larger the value, the higher the skin keratin layer moisture content, that is, the better the moisturizing effect of the product. Based on the comparison of the values of each parameter reference value (D0) and the revisit time point (D0T4h and D0T8h).

[0112] The test results are shown in Figure 5 , and the results show that compared with the reference value (D0), the skin keratin layer moisture content increases by 45.87% and 48.92% at D0T4h and D0T8h, respectively. According to the analysis of variance, there is a significant difference in the skin keratin layer moisture content at the revisit time points of D0T4h and D0T8h compared with D0 (p<0.001); compared with the blank group: (D0T4h-D0) and (D0T8h-D0), the skin keratin layer moisture content of the sample group has a significant difference (p<0.001), which shows that the soothing essence water has a moisturizing effect.

[0113] Zebrafish baby and child cosmetic safety test

[0114] Test sample: soothing essence water of Example 1​

[0115] Test object: wild type AB strain zebrafish, fish age: 2 days post fertilization (2dpf), number of experimental samples in each group: 30;

[0116] The skin structure and function of infants are quite different from those of adults, mainly manifested in that the infant skin is relatively smooth, has a faster renewal rate, and is easily damaged due to a weak barrier function. Therefore, the focus of infant skin care products is the safety of the products. Zebrafish has a high similarity of 87% to the human genome, has similar toxicity characteristics and signal transduction pathways to humans, and is a relatively strong comparable model organism, which can be used for safety evaluation of infant and child cosmetics of various types of samples.

[0117] The test method comprises the following steps:

[0118] (1) Randomly select zebrafish in a 6-well plate, 30 per well;

[0119] (2) Water-soluble administration of samples, while setting up a normal control group, 3mL per well.

[0120] (3) Incubate at 28℃ in the dark for 72h, and count and timely remove dead zebrafish every day during the period.

[0121] (4) After the end of the experiment, observe and record the reaction of the heart, circulatory system, hemorrhage and thrombus, brain, mandible, eye, liver, kidney, intestine, trunk / tail / notochord, muscle / segment, body color, body length, etc. of zebrafish in each experimental group under a dissecting microscope, and collect photos of typical toxic target organs. According to the death of zebrafish and the toxic reaction of each organ, the safety of the sample is evaluated.

[0122] The test results are shown in Table 5 and Figure 6 According to the results, the mortality rate of the Shu Min serum sample group is 0, and the tissue and organ morphology of the Shu Min serum sample group is similar to that of the normal control group, indicating that the Shu Min serum sample has no toxic risk and good safety.

[0123] Table 5 Safety test results of the Shu Min serum of the application

[0124] Sample name Detection concentration (%) Mortality rate (%) Phenotype Detection result Shu Min essence water 0.5 0 No obvious abnormality Safe

[0125] Test Example 7 Human skin patch test

[0126] Test sample: Shu Min serum of Example 1

[0127] Subjects: 30 volunteers aged 21 to 53 years old, 6 men and 24 women, who have not used hormone drugs and immunosuppressants in the past month;

[0128] Test method: refer to the method in the "Cosmetic Safety Technology Standard 2015 Edition" for testing, select qualified spot tester, with closed spot test method, about 0.020ml-0.025ml of the test object is placed in the spot tester, the outer low sensitization adhesive tape is pasted on the back of the test subject, 24 hours later, remove the test object, respectively at 0.5, 24, 48 hours after removal, observe the skin reaction, according to the method in the "Cosmetic Safety Technology Standard 2015 Edition" for skin reaction grading standard record its results. The results are shown in Table 6.

[0129] From Table 6, 0 cases of skin adverse reactions occurred in 30 people, indicating that the soothing essence water of the present application has no adverse effect on the skin, and has good safety.

[0130] Table 6: Results of cosmetic human skin patch test

[0131]

[0132] In summary, the composition with soothing sensitive skin effect of the present application has soothing effect, anti-inflammatory effect and moisturizing and repairing effect similar to steroid drugs, is green and safe, is easily accepted by allergic people and has high safety.

[0133] The foregoing description of specific exemplary embodiments of the present application is intended for purposes of illustration and example only. These descriptions are not intended to limit the application to the precise form disclosed, and obviously many changes and modifications can be suggested to one skilled in the art without departing from the spirit or scope of the application. It is chosen and described exemplary embodiments in order to explain the particular principles of the application and its practical application, so that those skilled in the art can realize and utilize various different exemplary embodiments of the present application and various different choices and changes. The scope of the present application is intended to be limited by the claims and their equivalents.

Claims

1. A composition having soothing sensitive skin efficacy, characterized in that, The composition comprises the following components in percentage by mass: 10-12% of plant extract, 2-4% of skin repair factor, 10% of jasmine hydrolat, 6-7% of moisturizing agent, and the balance of water; the plant extract comprises Sophora flavescens extract, Semen Vaccariae extract, and Dictamnus dasycarpus extract, and the weight ratio of the Sophora flavescens extract, Semen Vaccariae extract, and Dictamnus dasycarpus extract is 3-5:3-5:2-2.8, and the Sophora flavescens extract and the Semen Vaccariae extract are used in the same amount; the skin repair factor comprises the following components: β-glucan, nicotinamide, ectoine, squalane, sodium hyaluronate, and allantoin, and the percentage by mass of each component in the composition is as follows: 0.2-0.4% of β-glucan, 1.3-2.5% of nicotinamide, 0.08-0.12% of ectoine, 0.3-0.8% of squalane, 0.04-0.06% of sodium hyaluronate, and 0.08-0.12% of allantoin.

2. The composition for soothing sensitive skin according to claim 1, wherein The weight ratio of the Sophora flavescens extract, Semen Vaccariae extract, and Dictamnus dasycarpus extract is 4:4:2.

5.

3. The composition for soothing sensitive skin according to claim 1, wherein The percentage by mass of each component of the skin repair factor is as follows: 0.3% of β-glucan, 2% of nicotinamide, 0.1% of ectoine, 0.5% of squalane, 0.05% of sodium hyaluronate, and 0.1% of allantoin.

4. The composition for soothing sensitive skin according to claim 1, wherein The moisturizing agent comprises the following components: glycerol, 1,2-hexanediol, capryloyl hydroxamic acid, capryloyl glycine, and 1,3-propanediol.

5. A method for preparing a composition having soothing sensitive skin efficacy according to any one of claims 1 to 4, characterized in that, The method comprises the following steps: (1) mixing allantoin, ectoine, sodium hyaluronate, β-glucan, glycerol, and water to obtain phase A; (2) uniformly mixing 1,2-hexanediol, capryloyl hydroxamic acid, capryloyl glycine, 1,3-propanediol, nicotinamide, and squalane, and then mixing the mixture with plant extract and jasmine hydrolat to obtain phase B; (3) weighing phase A and adding it into a stirring pot, heating to 80-85°C, setting the stirring speed to 1200-1800 RPM, stirring for 20-40 minutes, homogenizing at 1500-2100 RPM for 5-7 minutes, and completely dissolving; (4) cooling to below 45°C, weighing phase B, adding it into the stirring pot, stirring at 1200-1800 RPM for 10-15 minutes, and uniformly mixing to obtain a composition having the effect of soothing sensitive skin.

6. Use of the composition having the effect of soothing sensitive skin according to any one of claims 1-4 in the preparation of a skin care product.

7. Use according to claim 6, wherein The skin care product is essence, cream, emulsion, or mask. The skin care product is essence, cream, emulsion, or mask.

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