Centrifugal separation device for plasma protein extraction

Abstract

The application belongs to the field of centrifugal separation devices, and provides a centrifugal separation device for extracting plasma protein, which comprises a centrifugal cylinder and further comprises a mechanism box, a fixing seat arranged at the bottom of the mechanism box, a rotating sleeve rotatably connected to the mechanism box, the centrifugal cylinder fixedly connected to the rotating sleeve, a plurality of suction cups connected to the fixing seat, and a driving mechanism arranged in the mechanism box; a plurality of positioning sleeves are annularly arranged around the rotating center of the rotating sleeve in the centrifugal cylinder, a supporting mechanism is arranged in the positioning sleeve, and the centrifugal cylinder is slidably connected to a limiting mechanism. When the air pressure in the positioning sleeve containing test tubes decreases, the rubber sleeve expands towards the inside of the positioning sleeve under the action of air pressure, so that the rubber sleeve is attached to the side wall of the test tube, thereby increasing the horizontal stability of the test tube, and the problem of poor stability when detecting raw plasma of blood products is solved.

Description

TECHNICAL FIELD

[0001] The present application belongs to the field of centrifugal separation devices, and particularly relates to a centrifugal separation device for extracting plasma protein. BACKGROUND

[0002] Plasma protein refers to the protein part in plasma, and is a general term for various proteins. Plasma protein can be divided into several components such as albumin, globulin and fibrinogen. Albumin has the smallest molecular weight among the three types of proteins, but its content in plasma is the highest. Blood products are biological products prepared by separating and extracting plasma protein from human plasma. In order to develop new blood product and improve the comprehensive utilization rate of plasma, centrifugal separation of human plasma protein is often required for component analysis and research. At present, more than 20 plasma protein components can be separated and purified, and divided into three categories: albumin, globulin and coagulation factor, to prepare blood products with good safety, high effectiveness and definite curative effect.

[0003] The centrifugal separation device is used for centrifugal separation of plasma protein. The existing centrifugal separation device usually has an elastic device built-in to increase the stability of the test tube during centrifugation of plasma protein. However, when the existing centrifugal separation device is centrifuged, the vibration force on the test tube increases with the increase of centrifugal force away from the rotation center, which leads to certain errors in the contrast results and affects the development and research efficiency of new blood products.

[0004] In order to avoid the above technical problems, it is necessary to provide a centrifugal separation device for extracting plasma protein to overcome the defects in the prior art. SUMMARY

[0005] The present application aims to provide a centrifugal separation device for extracting plasma protein, which aims to increase the stability of the test tube during centrifugation.

[0006] The present application is realized as follows: the centrifugal separation device for extracting plasma protein comprises a centrifuge cylinder, and further comprises:

[0007] The mechanism box is provided with a fixing seat at the bottom, the mechanism box is rotationally connected with a rotating sleeve, the rotating sleeve is fixedly connected with the centrifuge cylinder, the fixing seat is connected with a plurality of suction cups, and the mechanism box is provided with a driving mechanism, which can drive the rotating sleeve to rotate;

[0008] The centrifuge cylinder is annularly provided with a plurality of positioning sleeves around the rotation center of the rotating sleeve, the inside of the positioning sleeve is provided with a supporting mechanism, and the elastic supporting force of the supporting mechanism increases with the increase of centrifugal force;

[0009] The centrifuge cylinder is slidingly connected with a limiting mechanism, the limiting mechanism can position the test tube in the positioning sleeve, and the limiting mechanism can seal the positioning sleeve.

[0010] The side wall of the centrifugal cylinder is communicated with the rotating sleeve through an air passage, and the rotating sleeve is connected with a gas pressure mechanism, which can generate a negative pressure environment in the positioning sleeve when the rotating sleeve rotates;

[0011] The limiting mechanism is connected with an air bag assembly, which can limit the test tube under the action of the gas pressure mechanism.

[0012] Further technical solutions, the driving mechanism includes motor, driving wheel and driven wheel;

[0013] The motor is fixedly connected with the fixed seat, the driving wheel is fixedly connected with the output shaft of the motor, the driven wheel is fixedly connected with the rotating sleeve, and the driving wheel is engaged with the driven wheel.

[0014] Further technical solutions, the supporting mechanism includes support seat, spring, guide seat, push seat, fixed rod and eccentric wheel;

[0015] The support seat and the guide seat are vertically slidably connected with the inner wall of the positioning sleeve, and the support seat seals the port of the air passage, the spring is arranged between the support seat and the guide seat, the push seat is slidably and sealingly connected with the inner end surface of the positioning sleeve, and the push seat and the inclined surface of the guide seat abut each other, one end of the fixed rod is fixedly connected with the push seat, the other end of the fixed rod is fixedly connected with the eccentric wheel, and the centers of the eccentric wheels are away from the center of the centrifugal cylinder.

[0016] Further technical solutions, the limiting mechanism includes a limiting frame, a sealing sleeve, a limiting hole and a positioning assembly;

[0017] The limiting frame can be slidably connected with the inner wall of the centrifugal cylinder, a plurality of limiting holes are arranged on the limiting frame, one side of each limiting hole is communicated with a sealing sleeve, and the sealing sleeve can be vertically slidably and sealingly connected with the positioning sleeve;

[0018] The positioning assembly is arranged in the sealing sleeve, and the positioning assembly can elastically limit the test tube;

[0019] The limiting frame is rotatably connected with a threaded sleeve, a vertical rod is fixedly connected with the center of the centrifugal cylinder, and the threaded sleeve and the vertical rod can be threadedly connected.

[0020] Further technical solutions, the positioning assembly includes a spring and a ring sleeve, the ring sleeve is slidably and sealingly connected with the inner wall of the sealing sleeve, the spring is arranged between the limiting frame and the ring sleeve, and the ring sleeve can abut against the boss of the test tube.

[0021] Further technical solutions, the gas pressure mechanism includes a piston cylinder, a piston, a rotating tube, a thrust assembly and an elastic assembly;

[0022] The piston cylinder is fixedly connected with the fixed seat, the piston is slidably connected with the inner end surface of the piston cylinder, and the elastic assembly is connected between the piston and the piston cylinder;

[0023] The rotating pipe is communicated with the rotating sleeve, and the thrust assembly is fixedly connected with the rotating sleeve; when the rotating pipe rotates, the thrust assembly pushes the piston to vertically slide along the piston cylinder under the action of centrifugal force.

[0024] The technical scheme in one step, the thrust assembly includes a crossbar, a No. 3 spring and a counterweight;

[0025] The crossbar is fixedly connected with the rotating pipe, the counterweight is slidably connected with the crossbar through a bearing, the No. 3 spring is sleeved outside the crossbar, the piston is provided with a conical surface, and the counterweight abuts against the conical surface of the piston.

[0026] The technical scheme further, the elastic assembly includes a guide rod, a No. 4 spring, a spring baffle and an adjusting rod;

[0027] The adjusting rod is rotationally connected with the connecting seat of the outer end surface of the piston cylinder, the spring baffle is slidably connected with the side wall of the piston cylinder, and the spring baffle is threadedly connected with the adjusting rod;

[0028] The guide rod is fixedly connected with the piston, and the guide rod penetrates through the spring baffle, and the No. 4 spring is sleeved outside the guide rod.

[0029] The technical scheme further, the air bag assembly includes two arc-shaped sleeves, a rubber sleeve and air holes;

[0030] The two arc-shaped sleeves can be sleeved outside the sealing sleeve, and the two arc-shaped sleeves can be clamped between the two arc-shaped sleeves, the sealing sleeve is annularly provided with an arc-shaped end hole, the arc-shaped sleeve is connected with the rubber sleeve, the rubber sleeve passes through the arc-shaped end hole, and the arc-shaped sleeve is provided with a plurality of air holes.

[0031] Compared with the prior art, the beneficial effects of the present application are as follows:

[0032] 1、The centrifugal separation device for extracting plasma provided by the present application, when the rotating sleeve rotates, the pusher slides relative to the inner end surface of the positioning sleeve under the action of centrifugal force, the pusher pushes the guide seat, the guide seat drives the support seat to compress the No. 1 spring, so as to exert a vertical elastic thrust on the test tube, thereby increasing the vertical stability of the test tube; the elastic support force of the support seat increases with the increase of the centrifugal force, so as to further limit the test tube away from the rotation center, thereby increasing the stability of the test tube;

[0033] 2. The present invention provides a centrifugal separation device for extracting plasma proteins. The rotating sleeve drives the rotating tube to rotate, and the rotating tube drives the thrust assembly to push the piston. The piston moves vertically downward along the piston cylinder, thereby reducing the air pressure in the upper part of the piston cylinder. When the positioning sleeve contains the test tube, under the thrust of the test tube, the support seat moves downward and releases the seal on the air channel. The piston cylinder is connected to the positioning sleeve containing the test tube through the rotating tube, the rotating sleeve and the air channel, thereby reducing the air pressure in the positioning sleeve containing the test tube, and causing the ring in the sealing sleeve to move downward along the sealing sleeve, increasing the vertical positioning effect of the test tube.

[0034] 3. The centrifugal separation device for plasma protein extraction provided by the present invention, in the thrust assembly, when the rotating tube rotates, the counterweight moves horizontally along the crossbar, and the counterweight pushes the piston at the same time. When the rotation speed increases, the centrifugal force increases, thereby increasing the pushing distance of the counterweight on the piston, which in turn increases the vertical positioning force of the test tube and enhances the vertical positioning effect of the test tube.

[0035] 4. The centrifugal separation device for plasma protein extraction provided by the present invention has a fourth spring providing elastic support for the piston in the elastic component. When the adjusting rod is rotated, the adjusting rod drives the spring baffle to move vertically, and the spring baffle pushes the piston to move vertically. The contact force between the piston and the counterweight changes, thereby changing the piston movement distance at the same centrifugation rate, and thus changing the test tube positioning effect during centrifugation.

[0036] 5. The present invention provides a centrifugal separation device for extracting plasma proteins. When the air pressure in the positioning sleeve of the test tube decreases, the rubber sleeve expands into the positioning sleeve under the action of air pressure, thereby making the rubber sleeve fit against the side wall of the test tube, thereby increasing the horizontal stability of the test tube. Attached Figure Description

[0037] Figure 1 This is a schematic diagram of the structure of the present invention;

[0038] Figure 2 for Figure 1 Cross-sectional view of the centrifuge tube;

[0039] Figure 3 This is a sectional view of the mechanism box;

[0040] Figure 4 This is a cross-sectional view of the piston cylinder;

[0041] Figure 5 for Figure 2 Enlarged structural diagram of region A in the middle;

[0042] Figure 6 for Figure 2 Enlarged structural cross-sectional view of region B in the middle;

[0043] Figure 7 This is a schematic diagram of the limiting mechanism.

[0044] In the attached diagram: 1. Fixed base; 2. Mechanism box; 3. Rotating sleeve;

[0045] Drive mechanism; 41. Motor; 42. Driving wheel; 43. Driven wheel;

[0046] Support mechanism; 51. Support base; 52. No. 1 spring; 53. Guide seat; 54. Push seat; 55. Fixed rod; 56. Eccentric wheel;

[0047] Limiting mechanism; 61. Limiting frame; 62. Sealing sleeve; 63. Limiting hole; 64. Elastic component; 641. No. 2 spring; 642. Ring sleeve; 65. Threaded sleeve; 66. Vertical rod;

[0048] Pneumatic mechanism; 71. Piston cylinder; 72. Piston; 73. Rotary tube; 74. Thrust assembly; 741. Crossbar; 742. Spring No. 3; 743. Counterweight; 75. Elastic assembly; 751. Guide rod; 752. Spring No. 4; 753. Spring baffle; 754. Adjusting rod;

[0049] 8. Airbag assembly; 81. Arc-shaped sleeve; 82. Rubber sleeve; 83. Air vent;

[0050] 9. Test tube; 10. Air channel; 11. Arc-shaped end hole; 12. Centrifuge tube; 13. Suction cup; 14. Positioning sleeve. Detailed Implementation

[0051] To make the objectives, technical solutions, and advantages of this invention clearer, the invention will be further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative and not intended to limit the invention.

[0052] The specific implementation of the present invention will be described in detail below with reference to specific embodiments.

[0053] like Figures 1-7 As shown, a centrifugal separation device for plasma protein extraction provided by the present invention includes a centrifuge cylinder 12, and further includes:

[0054] The mechanism box 2 has a fixed base 1 at its bottom, a rotating sleeve 3 rotatably connected to the mechanism box 2, a centrifuge cylinder 12 fixedly connected to the rotating sleeve 3, a plurality of suction cups 13 connected to the fixed base 1, and a driving mechanism 4 provided in the mechanism box 2, which can drive the rotating sleeve 3 to rotate.

[0055] The centrifuge cylinder 12 has a plurality of positioning sleeves 14 arranged in a ring around the rotation center of the rotating sleeve 3. The positioning sleeve 14 has a support mechanism 5 inside, and the elastic support force of the support mechanism 5 increases with the increase of centrifugal force.

[0056] The centrifugal cylinder 12 is slidingly connected with a limiting mechanism 6, the limiting mechanism 6 can position the test tube 9 in the positioning sleeve 14, and the limiting mechanism 6 can seal the positioning sleeve 14;

[0057] The side wall of the centrifugal cylinder 12 is communicated with the air passage 10 through the rotating sleeve 3, the rotating sleeve 3 is connected with the air pressure mechanism 7, when the rotating sleeve 3 rotates, the air pressure mechanism 7 can generate a negative pressure environment in the positioning sleeve 14;

[0058] The limiting mechanism 6 is connected with the air bag assembly 8, under the action of the air pressure mechanism 7, the air bag assembly 8 can limit the test tube 9.

[0059] In use, open the centrifugal cylinder 12, take out the limiting mechanism 6, then put the test tube 9 containing plasma into the positioning sleeve 14 in sequence; then put the limiting mechanism 6 into the centrifugal cylinder 12, the limiting mechanism 6 can position the test tube 9 in the positioning sleeve 14, and the limiting mechanism 6 can seal the positioning sleeve 14;

[0060] Close the centrifugal cylinder 12, start the driving mechanism 4, the driving mechanism 4 can drive the rotating sleeve 3 to rotate, so as to centrifuge the plasma in the test tube 9, when the rotating sleeve 3 rotates, the air pressure mechanism 7 can generate a negative pressure environment in the positioning sleeve 14, so as to suck the limiting mechanism 6 and the positioning sleeve 14 tightly, thereby increasing the stability of the test tube 9, and under the action of the air pressure mechanism 7, the air bag assembly 8 can limit the test tube 9;

[0061] The elastic supporting force of the supporting mechanism 5 increases with the increase of the centrifugal force, so as to further limit the test tube 9 away from the rotation center, thereby increasing the stability of the test tube 9.

[0062] In the embodiment of the application, as a preferred embodiment of the application, the driving mechanism 4 comprises a motor 41, a driving wheel 42 and a driven wheel 43;

[0063] The motor 41 is fixedly connected with the fixed seat 1, the driving wheel 42 is fixedly connected with the output shaft of the motor 41, the driven wheel 43 is fixedly connected with the rotating sleeve 3, and the driving wheel 42 is engaged with the driven wheel 43.

[0064] In the driving mechanism 4, the motor 41 is started, the motor 41 drives the driving wheel 42 to rotate, and the driving wheel 42 drives the rotating sleeve 3 to rotate through the engagement with the driven wheel 43.

[0065] In the embodiment of the application, as a preferred embodiment of the application, the supporting mechanism 5 comprises a supporting seat 51, a first spring 52, a guide seat 53, a push seat 54, a fixed rod 55 and an eccentric wheel 56;

[0066] The support seat 51 and the guide seat 53 are vertically slidably connected with the inner wall of the positioning sleeve 14, the support seat 51 seals the port of the air passage 10, the first spring 52 is arranged between the support seat 51 and the guide seat 53, the push seat 54 is slidably and sealingly connected with the inner end surface of the positioning sleeve 14, the push seat 54 abuts against the inclined surface of the guide seat 53, one end of the fixing rod 55 is fixedly connected with the push seat 54, the other end of the fixing rod 55 is fixedly connected with the eccentric wheel 56, and the centers of the eccentric wheels 56 are away from the center of the centrifugal cylinder 12.

[0067] In the supporting mechanism 5, the first spring 52 pushes the support seat 51 to elastically support the test tube 9, when the centrifugal cylinder 12 rotates, under the action of the centrifugal force, the push seat 54 slides relative to the inner end surface of the positioning sleeve 14, the push seat 54 pushes the guide seat 53, the guide seat 53 drives the support seat 51 to compress the first spring 52, so that a vertical elastic pushing force is applied to the test tube 9, and the vertical stability of the test tube 9 is increased.

[0068] In the embodiment of the application, as a preferred embodiment of the application, the limiting mechanism 6 comprises a limiting frame 61, a sealing sleeve 62, a limiting hole 63 and a positioning assembly 64.

[0069] The limiting frame 61 can be slidably connected with the inner wall of the centrifugal cylinder 12, a plurality of limiting holes 63 are arranged on the limiting frame 61, the limiting holes 63 are communicated with the sealing sleeves 62 on one side, and the sealing sleeves 62 can be vertically and sealingly connected with the positioning sleeve 14.

[0070] The positioning assembly 64 is arranged in the sealing sleeve 62, and the positioning assembly 64 can elastically limit the test tube 9.

[0071] The limiting frame 61 is rotationally connected with a threaded sleeve 65, a vertical rod 66 is fixedly connected with the center position of the centrifugal cylinder 12, and the threaded sleeve 65 can be threadedly connected with the vertical rod 66.

[0072] In the limiting mechanism 6, the limiting frame 61 is placed into the centrifugal cylinder 12, the threaded sleeve 65 is rotated, the threaded sleeve 65 moves downward along the vertical rod 66, the sealing sleeve 62 is arranged outside the positioning sleeve 14 and is sealingly connected with the positioning sleeve 14, and the positioning assembly 64 can elastically limit the test tube 9.

[0073] In the embodiment of the application, as a preferred embodiment of the application, the positioning assembly 64 comprises a second spring 641 and a ring sleeve 642, the ring sleeve 642 is slidably and sealingly connected with the inner wall of the sealing sleeve 62, the second spring 641 is arranged between the limiting frame 61 and the ring sleeve 642, and the ring sleeve 642 can abut against the boss of the test tube 9.

[0074] In the positioning assembly 64, the second spring 641 pushes the ring 642 to abut against the boss of the test tube 9, so as to vertically position the test tube 9.

[0075] In the embodiment of the present application, as a preferred embodiment of the present application, the air pressure mechanism 7 comprises a piston cylinder 71, a piston 72, a rotating tube 73, a thrust assembly 74 and an elastic assembly 75.

[0076] The piston cylinder 71 is fixedly connected with the fixed seat 1, the piston 72 is in sliding connection with the inner end face of the piston cylinder 71, and the elastic assembly 75 is connected between the piston 72 and the piston cylinder 71.

[0077] The rotating tube 73 is in communication with the rotating sleeve 3, and the thrust assembly 74 is fixedly connected with the rotating sleeve 3; when the rotating tube 73 rotates, under the action of centrifugal force, the thrust assembly 74 pushes the piston 72 to vertically slide along the piston cylinder 71.

[0078] In the air pressure mechanism 7, the rotating sleeve 3 drives the rotating tube 73 to rotate, the rotating tube 73 drives the thrust assembly 74 to push the piston 72, and the piston 72 vertically moves downward along the piston cylinder 71, so that the air pressure in the upper part of the piston cylinder 71 is reduced; when the positioning sleeve 14 contains the test tube 9, under the pushing force of the test tube 9, the support seat 51 moves downward and simultaneously releases the sealing of the air passage 10, the piston cylinder 71 is in communication with the positioning sleeve 14 containing the test tube 9 through the rotating tube 73, the rotating sleeve 3 and the air passage 10, so that the air pressure in the positioning sleeve 14 containing the test tube 9 is reduced, and then the ring 642 in the sealing sleeve 62 moves downward along the sealing sleeve 62, so as to increase the vertical positioning of the test tube 9.

[0079] In the embodiment of the present application, as a preferred embodiment of the present application, the thrust assembly 74 comprises a cross rod 741, a third spring 742 and a counterweight 743.

[0080] The cross rod 741 is fixedly connected with the rotating tube 73, the counterweight 743 is in sliding connection with the cross rod 741 through a bearing, the third spring 742 is sleeved outside the cross rod 741, the piston 72 is provided with a conical surface, and the counterweight 743 abuts against the conical surface of the piston 72.

[0081] In the thrust assembly 74, when the rotating tube 73 rotates, the counterweight 743 moves horizontally along the cross rod 741, and the counterweight 743 simultaneously pushes the piston 72; when the rotating speed increases, the centrifugal force increases, so that the pushing distance of the counterweight 743 to the piston 72 increases, and then the vertical positioning force of the test tube 9 increases, so as to increase the vertical positioning effect of the test tube 9.

[0082] In the embodiment of the present application, as a preferred embodiment of the present application, the elastic assembly 75 comprises a guide rod 751, a fourth spring 752, a spring baffle 753 and an adjusting rod 754.

[0083] The adjusting rod 754 is rotationally connected with the connecting base of the outer end surface of the piston cylinder 71, the spring baffle 753 is slidingly connected with the side wall of the piston cylinder 71, and the spring baffle 753 is threadedly connected with the adjusting rod 754;

[0084] The guide rod 751 is fixedly connected with the piston 72, and the guide rod 751 penetrates through the spring baffle 753, and the fourth spring 752 is sleeved outside the guide rod 751.

[0085] In the elastic assembly 75, the fourth spring 752 elastically supports the piston 72, the adjusting rod 754 is rotated, the adjusting rod 754 drives the spring baffle 753 to move vertically, the spring baffle 753 drives the piston 72 to move vertically, the abutting force between the piston 72 and the counterweight 743 is changed, so that the moving distance of the piston 72 is changed at the same centrifugal rate, and then the positioning effect of the test tube 9 during centrifugation is changed.

[0086] In the embodiment of the application, as a preferred embodiment of the application, the air bag assembly 8 comprises two arc-shaped sleeves 81, a rubber sleeve 82 and air holes 83.

[0087] The two arc-shaped sleeves 81 can be sleeved outside the sealing sleeve 62, and the two arc-shaped sleeves 81 can be clamped between the two arc-shaped sleeves 81, the sealing sleeve 62 is annularly provided with an arc-shaped end hole 11, the arc-shaped sleeve 81 is connected with the rubber sleeve 82, the rubber sleeve 82 penetrates through the arc-shaped end hole 11, and the arc-shaped sleeve 81 is provided with a plurality of air holes 83.

[0088] When the air pressure in the positioning sleeve 14 containing the test tube 9 decreases, the rubber sleeve 82 expands towards the inside of the positioning sleeve 14 under the action of the air pressure, so that the rubber sleeve 82 is attached to the side wall of the test tube 9, and then the horizontal stability of the test tube 9 is increased.

[0089] Working principle:

[0090] In use, the centrifugal cylinder 12 is opened, the limiting mechanism 6 is taken out, then the test tube 9 containing plasma protein is sequentially placed into the positioning sleeve 14, then the limiting mechanism 6 is placed into the centrifugal cylinder 12, the threaded sleeve 65 is rotated and moves downwards along the vertical rod 66, the sealing sleeve 62 is sleeved outside the positioning sleeve 14, and the sealing sleeve 62 is sealingly connected with the positioning sleeve 14, the second spring 641 drives the ring sleeve 642 to abut against the boss of the test tube 9, so that the test tube 9 is vertically limited.

[0091] The centrifugal cylinder 12 is closed, the motor 41 is started, the motor 41 drives the driving wheel 42 to rotate, the driving wheel 42 drives the rotating sleeve 3 to rotate through meshing with the driven wheel 43, so that the plasma protein in the test tube 9 is centrifuged.

[0092] When the rotating sleeve 3 rotates, the push seat 54 slides relative to the inner end surface of the positioning sleeve 14 under the action of centrifugal force, the push seat 54 pushes the guide seat 53, the guide seat 53 drives the support seat 51 to compress the first spring 52, so as to exert a vertical elastic pushing force on the test tube 9, thereby increasing the vertical stability of the test tube 9; the elastic supporting force of the support seat 51 increases with the increase of the centrifugal force, thereby further limiting the test tube 9 away from the rotation center, thereby increasing the stability of the test tube 9;

[0093] The rotating sleeve 3 drives the rotating tube 73 to rotate, the rotating tube 73 drives the pushing assembly 74 to push the piston 72, the piston 72 moves vertically downward along the piston cylinder 71, so that the air pressure in the upper part of the piston cylinder 71 decreases; when the positioning sleeve 14 contains the test tube 9, the support seat 51 moves downward while releasing the sealing of the air passage 10 under the pushing force of the test tube 9, the piston cylinder 71 communicates with the positioning sleeve 14 containing the test tube 9 through the rotating tube 73, the rotating sleeve 3 and the air passage 10, so that the air pressure in the positioning sleeve 14 containing the test tube 9 decreases, thereby making the ring sleeve 642 in the sealing sleeve 62 move downward along the sealing sleeve 62, increasing the vertical positioning of the test tube 9;

[0094] In the pushing assembly 74, when the rotating tube 73 rotates, the counterweight 743 moves horizontally along the cross rod 741, and the counterweight 743 simultaneously pushes the piston 72; when the rotating speed increases, the centrifugal force increases, thereby increasing the pushing distance of the counterweight 743 to the piston 72, and further increasing the vertical positioning force of the test tube 9, thereby increasing the vertical positioning effect of the test tube 9;

[0095] In the elastic assembly 75, the fourth spring 752 elastically supports the piston 72, the rotating adjusting rod 754 drives the spring baffle 753 to move vertically, the spring baffle 753 pushes the piston 72 to move vertically, the abutting force between the piston 72 and the counterweight 743 changes, so that the moving distance of the piston 72 changes at the same centrifugal rate, thereby changing the positioning effect of the test tube 9 during centrifugation;

[0096] When the air pressure in the positioning sleeve 14 containing the test tube 9 decreases, the rubber sleeve 82 expands towards the inside of the positioning sleeve 14 under the action of air pressure, so as to make the rubber sleeve 82 fit the side wall of the test tube 9, thereby increasing the horizontal stability of the test tube 9.

[0097] The above only describes the preferred embodiments of the present application, and is not intended to limit the present application, any modification, equivalent replacement and improvement made within the spirit and principle of the present application shall be included in the protection scope of the present application.

[0098] Furthermore, it should be understood that although the specification is described in terms of embodiments, not every embodiment includes every feature or implementation described herein. The specification can include implicit combinations of explicitly mentioned features and / or implicit combinations of implicitly mentioned features. Such combinations are also expressly included within the scope of the specification and an embodiment.

Claims

1. Centrifugal separation device for the extraction of plasma proteins, comprising a centrifugation cartridge (12), characterized in that, Also include: Mechanism box (2), the mechanism box (2) bottom is provided with fixed seat (1), the mechanism box (2) rotationally connected with rotating sleeve (3), the rotating sleeve (3) is fixedly connected with centrifugal cylinder (12), the fixed seat (1) is connected with a plurality of suction cups (13), the mechanism box (2) is provided with drive mechanism (4), the drive mechanism (4) can drive rotating sleeve (3) rotation; The rotating center of the rotating sleeve (3) is annularly provided with a plurality of positioning sleeves (14) in the centrifugal cylinder (12), the inside of the positioning sleeve (14) is provided with a supporting mechanism (5), and the elastic supporting force of the supporting mechanism (5) increases with the increase of centrifugal force; The centrifugal cylinder (12) is slidably connected with a limiting mechanism (6), the limiting mechanism (6) can position the test tube (9) in the positioning sleeve (14), and the limiting mechanism (6) can seal the positioning sleeve (14); The side wall of the centrifugal cylinder (12) is communicated with the rotating sleeve (3) with an air passage (10), the rotating sleeve (3) is connected with a gas pressure mechanism (7), when the rotating sleeve (3) rotates, the gas pressure mechanism (7) can generate a negative pressure environment in the positioning sleeve (14); The limiting mechanism is connected with an air bag assembly (8), under the action of the gas pressure mechanism (7), the air bag assembly (8) can limit the test tube (9); The supporting mechanism (5) comprises a supporting seat (51), a first spring (52), a guide seat (53), a push seat (54), a fixed rod (55) and an eccentric wheel (56); The supporting seat (51) and the guide seat (53) are vertically slidably connected with the inner wall of the positioning sleeve (14), and the supporting seat (51) seals the port of the air passage (10), the first spring (52) is arranged between the supporting seat (51) and the guide seat (53), the push seat (54) is slidably and sealingly connected with the inner end surface of the positioning sleeve (14), and the push seat (54) and the inclined surface of the guide seat (53) abut each other, one end of the fixed rod (55) is fixedly connected with the push seat (54), the other end of the fixed rod (55) is fixedly connected with the eccentric wheel (56), and the centers of the eccentric wheels (56) are away from the center of the centrifugal cylinder (12); The limiting mechanism (6) comprises a limiting frame (61), a sealing sleeve (62), a limiting hole (63) and a positioning assembly (64); The limiting frame (61) can be slidably connected with the inner wall of the centrifugal cylinder (12), a plurality of limiting holes (63) are arranged on the limiting frame (61), one side of the limiting hole (63) is communicated with the sealing sleeve (62), and the sealing sleeve (62) can be vertically slidably and sealingly connected with the positioning sleeve (14); The sealing sleeve (62) is provided with a positioning assembly (64), and the positioning assembly (64) can elastically limit the test tube (9); The limiting frame (61) is rotationally connected with a threaded sleeve (65), the center of the centrifugal cylinder (12) is fixedly connected with a vertical rod (66), and the threaded sleeve (65) and the vertical rod (66) can be threadedly connected. The positioning assembly (64) comprises a second spring (641) and a ring sleeve (642) which is in sliding sealing connection with the inner wall of the sealing sleeve (62), and the second spring (641) is arranged between the limiting frame (61) and the ring sleeve (642), and the ring sleeve (642) can abut against the boss of the test tube (9).

2. The centrifugal separation device for plasma protein extraction according to claim 1, characterized in that The driving mechanism (4) comprises a motor (41), a driving wheel (42) and a driven wheel (43); The motor (41) is fixedly connected with the fixed seat (1), the driving wheel (42) is fixedly connected with the output shaft of the motor (41), the driven wheel (43) is fixedly connected with the rotating sleeve (3), and the driving wheel (42) is engaged with the driven wheel (43).

3. The centrifugal separation device for plasma protein extraction according to claim 2, characterized in that The air pressure mechanism (7) comprises a piston cylinder (71), a piston (72), a rotating pipe (73), a thrust assembly (74) and an elastic assembly (75); The piston cylinder (71) is fixedly connected with the fixed seat (1), the piston (72) is in sliding connection with the inner end surface of the piston cylinder (71), and the elastic assembly (75) is connected between the piston (72) and the piston cylinder (71); The rotating pipe (73) is in communication with the rotating sleeve (3), the thrust assembly (74) is fixedly connected with the rotating sleeve (3), and when the rotating pipe (73) rotates, under the action of centrifugal force, the thrust assembly (74) pushes the piston (72) to vertically slide along the piston cylinder (71).

4. The plasma protein extraction centrifugation separation device of claim 3, wherein, The thrust assembly (74) comprises a cross rod (741), a third spring (742) and a counterweight (743); The cross rod (741) is fixedly connected with the rotating pipe (73), the counterweight (743) is in sliding connection with the cross rod (741) through a bearing, the third spring (742) is sleeved outside the cross rod (741), the piston (72) is provided with a conical surface, and the counterweight (743) abuts against the conical surface of the piston (72).

5. The plasma protein extraction centrifugation separation device of claim 4, wherein, The elastic assembly (75) comprises a guide rod (751), a fourth spring (752), a spring baffle (753) and an adjusting rod (754); The adjusting rod (754) is rotationally connected with the connecting seat of the outer end surface of the piston cylinder (71), the spring baffle (753) is in sliding connection with the side wall of the piston cylinder (71), and the spring baffle (753) is in threaded connection with the adjusting rod (754); The guide rod (751) is fixedly connected with the piston (72), and the guide rod (751) penetrates through the spring baffle (753), and the fourth spring (752) is sleeved outside the guide rod (751).

6. The plasma protein extraction centrifugation separation device of claim 1, wherein, The air bag assembly (8) comprises two arc sleeves (81), a rubber sleeve (82) and air holes (83); The two arc sleeves (81) can be sleeved outside the sealing sleeve (62), and the two arc sleeves (81) can be clamped between them, the sealing sleeve (62) is annularly provided with an arc-shaped end hole (11), the arc sleeve (81) is connected with the rubber sleeve (82), the rubber sleeve (82) passes through the arc-shaped end hole (11), and a plurality of air holes (83) are arranged on the arc sleeve (81).

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