Dipeptidyl peptidase 4 biosensors, methods of making and using the same

By synthesizing a bioluminescent probe, high-sensitivity detection of dipeptidyl peptidase 4 is achieved using luciferase and ATP, solving the problems of low accuracy and complexity in existing detection methods. This method achieves high selectivity and sensitivity for the detection of dipeptidyl peptidase 4, and is suitable for labeling and imaging of various tumor cells.

CN117229276BActive Publication Date: 2026-06-12INST OF CHEM CHINESE ACAD OF SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
INST OF CHEM CHINESE ACAD OF SCI
Filing Date
2022-06-06
Publication Date
2026-06-12

AI Technical Summary

⚠Technical Problem

Existing dipeptidyl peptidase-4 detection methods suffer from low accuracy, long detection time, and complex operation, making it difficult to achieve simple, rapid, and efficient quantitative detection.

⚗Method used

A class of bioluminescent probes was designed and synthesized. By introducing glycine-L-proline groups specific to dipeptidyl peptidase 4 into D-luciferin-like substances, bioluminescence is generated under the action of luciferase, ATP and Mg2+, achieving highly sensitive detection of dipeptidyl peptidase 4.

🎯Benefits of technology

It achieves highly selective and sensitive detection of dipeptidyl peptidase 4, and is suitable for labeling and imaging tumor cells such as prostate cancer, gastric cancer, colorectal cancer, malignant mesothelioma, and liver cancer, with specificity and high efficiency.

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Abstract

The application discloses a kind of formula (I) shown for dipeptidyl peptidase 4 detection bioluminescence probe and its preparation method and application.The probe has good selectivity and sensitivity to dipeptidyl peptidase 4, and can be used as dipeptidyl peptidase 4 bioluminescence probe for marking prostate cancer, gastric cancer, colorectal cancer, malignant mesothelioma, liver cancer and a variety of tumor cells.
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