A polygonum multiflorum thumb extract, its preparation method and use

Abstract

The application discloses a preparation method of a polygonatum officinale extract. The method uses high-pressure homogenization extraction, macroporous adsorption resin purification and three-phase extraction technology to obtain total flavones and total polysaccharides with high purity. The extract is rich in two categories of chemical components and has the advantages of high extraction efficiency. The application further discloses the use of the polygonatum officinale extract in the preparation of an alcoholism-relieving medicine or health-care food. The polygonatum officinale extract prepared by the application can effectively inhibit the damage of alcohol to the gastric mucosa and the liver, and shorten the drunk maintaining time, and therefore can be used for the preparation of an alcoholism-relieving medicine or health-care food.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of medicine, and particularly relates to a Polygonatum sibiricum extract, a preparation method thereof and an application thereof in preparing an alcoholism-relieving medicine. BACKGROUND

[0002] The history of wine is long and far-reaching. China is a big country in the production and consumption of wine beverages. With the gradual improvement of people's material living standards, the consumption of alcoholic beverages is also increasing, and the incidence of adult alcoholism is also increasing. In addition, long-term drinking can cause damage to multiple systems of the body, especially the gastrointestinal tract, and liver damage is more obvious. Alcohol can directly or indirectly cause chronic gastritis, peptic ulcer and alcoholic cirrhosis. Therefore, for a long time, people have been using various methods to relieve and reduce the physical harm caused by alcohol.

[0003] In order to prevent and alleviate the harm caused by alcohol to the body, people have developed various foods or medicines to reduce damage to the gastrointestinal tract and liver, and to wake people up from a drunken state more quickly and effectively, restore consciousness or restore self-control. Polygonatum sibiricum is the dried rhizome of Polygonatum odoratum (Mill.) Druce of the plant of the genus Polygonatum in the Liliaceae family, which was first recorded in Shennong's Herbal Classic and was listed as a superior product. It is a commonly used traditional Chinese medicinal material in China. Polygonatum sibiricum mainly contains flavonoids, steroidal saponins, sterols, polysaccharides, amino acids, volatile oils, trace elements and other components, and has the effects of nourishing yin and moistening dryness, generating fluid to relieve thirst, and anti-aging. Modern research shows that Polygonatum sibiricum has various pharmacological effects such as expanding coronary arteries, lowering blood lipids, lowering blood sugar and enhancing immunity. In addition, Polygonatum sibiricum also has antioxidant and hepatocyte protection activities, and is therefore often added to alcoholism-relieving medicines or food and beverages.

[0004] However, the active ingredients and pharmacological effects of Polygonatum sibiricum for relieving alcoholism are not completely clear at present, resulting in that the existing alcoholism-relieving medicine preparation process is backward, and the alcoholism-relieving effect cannot be fully exerted. Therefore, the applicant selects two large categories of active ingredients, total flavonoids and total polysaccharides, and conducts in-depth research on the extraction method and pharmacological effects, and finally realizes the present application. SUMMARY

[0005] The first object of the present application is to provide a preparation method of a Polygonatum sibiricum extract. The method combines high-pressure homogenization extraction, macroporous adsorption resin purification and three-phase extraction technology, and high-purity total flavonoids and total polysaccharides are obtained. Compared with the existing extraction method, the extract prepared by the present application is rich in two categories of chemical components, i.e. total flavonoids including quercetin and dihydrogen high isoflavones and total polysaccharides, and the extraction method has the advantages of mild extraction conditions, high extraction efficiency and high biological activity of the extracted components.

[0006] To achieve the first object, the technical scheme used by the present application is as follows:

[0007] A preparation method of a polygonatum extract, comprising the following steps:

[0008] S1, crushing polygonatum officinale herb, then adding 60-90% ethanol for high-pressure homogenization extraction, after the extraction is completed, first concentrating and drying the extract, then dissolving with water and preparing into a 10-50 mg / ml crude extract, loading the crude extract onto a macroporous resin column, first eluting with water until colorless, then eluting with 60-90% ethanol as eluent, collecting the ethanol eluate, concentrating and drying to obtain a polygonatum officinale alcohol extract;

[0009] S2, crushing polygonatum officinale herb, then adding water for high-pressure homogenization extraction, after the extraction is completed, first concentrating the extract, then adding tert-butyl alcohol and ammonium sulfate to the concentrated solution, stirring and then standing for layer separation, collecting the lower aqueous phase containing polysaccharides, removing ammonium sulfate and then freeze-drying to obtain a polygonatum officinale water extract;

[0010] S3, mixing the alcohol extract and the water extract in a proportion to obtain a polygonatum officinale extract rich in total flavonoids and total polysaccharides, wherein the total flavonoids include quercetin and dihydrogen high isoflavones.

[0011] Preferably, the pressure of the high-pressure homogenization extraction is 200-700 bar.

[0012] Preferably, the filler of the macroporous resin column is HPD-100 type macroporous resin.

[0013] Preferably, the loading amount of the crude extract onto the column is 3-8 BV, and the flow rate of the eluent is 1-3 BV / h.

[0014] Preferably, the ratio of the concentrated solution to ammonium sulfate to tert-butyl alcohol is (3-8):(0.5-1.5):(5-10) ml / g / ml.

[0015] Preferably, the temperature for standing for layer separation is 2-8°C.

[0016] According to one embodiment of the present application, a better preparation method is as follows:

[0017] S1, crushing polygonatum officinale herb through a 60-mesh sieve, then adding 70% ethanol at 10 times the weight of the herb, and performing high-pressure homogenization extraction under a pressure of 400 bar, a total of 2 times, 4 times of homogenization for each time, after the extraction is completed, combining the extract and concentrating and drying to obtain a crude extract, dissolving the crude extract with water and preparing into a 20 mg / ml crude extract, then loading onto a HPD-100 type macroporous resin column, the loading amount is 5 BV, after adsorption equilibrium, first eluting with water until colorless, then eluting with 90% ethanol, the flow rate of the eluent is 1 BV / h, collecting the ethanol eluate, concentrating and drying to obtain a polygonatum officinale alcohol extract;

[0018] S2, the polygonatum officinale medicinal material is crushed through a 60 mesh sieve, then water is added in 10 times of the medicinal material weight, high pressure homogenization extraction is carried out under the condition of pressure 400 bar, a total of 2 times of extraction, the homogenization times of each extraction is 5 times, after the extraction is finished, the extraction liquid is combined and concentrated to 1 / 50 of the original volume, then tert-butyl alcohol and ammonium sulfate are added to the concentrated liquid, the ratio of the concentrated liquid, ammonium sulfate and tert-butyl alcohol is 5:1:7.5 (ml / g / ml), after stirring for 2 hours, static layering is carried out at 2-8 DEG C, the lower water phase containing polysaccharide is collected, after removing ammonium sulfate, freeze-drying is carried out, the polygonatum officinale water extract is obtained;

[0019] S3, the polygonatum officinale alcohol extract and the polygonatum officinale water extract are mixed in a weight ratio of 3:1, the polygonatum officinale extract rich in total flavonoids and total polysaccharide is obtained, wherein the total flavonoids include quercetin and dihydrogen high isoflavones.

[0020] Using the above-mentioned optimized process parameters, the content of flavonoid components such as quercetin and dihydrogen high isoflavones in the polygonatum officinale alcohol extract and the content of polysaccharide components in the water extract can be further improved, and the mixture of the two has higher alcoholism relieving activity.

[0021] The polygonatum officinale extract prepared in the application is mixed by the water extract and the alcohol extract in a suitable ratio, the chemical components contained in the two extracts are different, wherein the alcohol extract mainly contains various flavonoid chemical components, and the water extract mainly contains various polysaccharide chemical components, so that the final product is rich in two categories of chemical components, i.e. total flavonoids and total polysaccharide. Flavonoids are a kind of components with relatively clear activity in polygonatum officinale, mainly including quercetin and dihydrogen high isoflavones, which have various pharmacological effects such as antioxidant and anti-inflammatory. Total polysaccharide has been proved to be a key active component in polygonatum officinale, and researches show that it has important pharmacological activities such as immune regulation and anti-tumor. The extract prepared in the application is rich in various components, so it is more beneficial to the exertion of curative effect.

[0022] In the preparation process of the Polygonatum multiflorum Thunb extract, we first use high-pressure homogenization extraction technology, which is a non-thermal processing technology, mainly using shearing, high-speed impact and other comprehensive effects to make the traditional Chinese medicinal materials achieve the effect of broken wall, greatly break the plant cell tissue structure, and accelerate the diffusion and penetration of soluble substances. This method has the advantages of high extraction efficiency, short extraction time, high biological activity of extracted components, etc. In addition, in the purification process of the Polygonatum multiflorum Thunb water extract, we first use a three-phase extraction method. The use of this method to purify polysaccharides can avoid the shortcomings of the conventional alcohol precipitation process (such as incomplete separation, low utilization rate of raw materials, large amount of ethanol, etc.), and the principle is to add inorganic salts and organic solvents to the water extract to form three phases. The upper phase is the organic layer, mainly containing small polar substances such as pigments and lipids; the middle phase is the protein layer; and the lower phase is the water layer, mainly containing water-soluble polysaccharides. By separating the lower water phase, the total polysaccharides can be obtained.

[0023] The second object of the present application is to provide a use of the Polygonatum multiflorum Thunb extract in the preparation of an alcoholism-relieving drug or health food.

[0024] To achieve the above-mentioned objects, the applicant administers the corresponding drug by gavage, then establishes an alcoholism mouse animal model and observes the drunken state of the mice in each group, and detects the gastric mucosa and liver damage degree of the mice. The results show that the Polygonatum multiflorum Thunb extract prepared by the present application can reduce the gastric mucosa damage index of the mice to a certain extent, reduce the serum glutamic-pyruvic transaminase and glutamic-oxalacetic transaminase, and shorten the maintenance time of the drunken state, indicating that the extract can effectively inhibit the damage of alcohol to the gastric mucosa and liver and has an alcoholism-relieving effect, and thus is expected to be used in the preparation of an alcoholism-relieving drug or health food.

[0025] In the preparation of the above-mentioned alcoholism-relieving drug or health food, the Polygonatum multiflorum Thunb alcohol extract and the Polygonatum multiflorum Thunb water extract are mixed at a weight ratio of 1-5:1. Since the effect of the mixture of the two extracts in alcoholism-relieving is obviously better than that of a single extract, it is proved that the total flavonoids and the total polysaccharides of Polygonatum multiflorum Thunb have a synergistic effect, and the mixing effect is better at a weight ratio of 3:1.

[0026] In the preparation of the above-mentioned alcoholism-relieving drug or health food, the Polygonatum multiflorum Thunb extract can be compounded with food raw materials or food additives or pharmaceutical excipients, and various preparations suitable for eating or clinical use can be prepared. Other active ingredients can also be added to the Polygonatum multiflorum Thunb extract to further enhance the drug efficacy. The food raw materials, food additives and pharmaceutical excipients include but are not limited to oat, bitter buckwheat, quinoa, chickpea, gordon euryale seed, yam, konjac powder, sesame, barley, wheat germ, wheat, coconut powder, wheat starch, corn starch, maltose, maltitol, gelatin, sodium caseinate, orange flavor, lemon flavor, lactose, mannitol, microcrystalline cellulose, Chinese plum flavor, white granulated sugar, glucose, fructose, inulin, maltose, xylitol, rebaudioside, chitosan, sorbitol, aspartame, neotame, mogroside, erythritol, etc.

[0027] The other active ingredients include, but are not limited to, Hericium erinaceus or its extract, bergamot or its extract, Lablab or its extract, Euryale or its extract, lily or its extract, Phyllanthus emblica or its extract, Tremella fuciformis or its extract, loquat flower or its extract, Marsdenia tenacissima or its extract, green tea or its extract, cysteine, dried tangerine or orange peel or its extract, Inula racemosa or its extract, taurine, collagen peptide, corn oligopeptide powder, Pueraria or its extract, Ampelopsis grossedentata or its extract, chrysanthemum or its extract, Maca or its extract, Moringa oleifera leaf or its extract, Spirulina, turmeric or its extract, tea flower or its extract, Acai berry or its extract, Tribulus terrestris or its extract, Ampelopsis grossedentata or its extract, loquat leaf or its extract, Eucommia ulmoides male flower or its extract, Herba Rubus suavissimus or its extract, grape seed or its extract, etc.

[0028] Finally, the present application also provides a specific alcoholism health care food formula which is mixed by Polygonatum odoratum extract, oat flour, tartary buckwheat flour, coconut powder, Hericium erinaceus powder, Tremella fuciformis powder, Lablab powder, malt powder, white granulated sugar, konjac powder, Pueraria powder, Phyllanthus emblica powder, Marsdenia tenacissima powder, chrysanthemum powder, bergamot powder.

[0029] Term explanation:

[0030] The ethanol concentration involved in the present application is all volume concentration, for example, 70% ethanol means that 70ml of ethanol is contained in 100ml of ethanol aqueous solution.

[0031] BV: refers to column volume, for example, 5BV refers to 5 times column volume. DETAILED DESCRIPTION

[0032] The technical solutions of the present application will be described clearly and completely in combination with specific embodiments. Obviously, the described embodiments are only part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor are within the protection scope of the present application.

[0033] Materials and instruments:

[0034] Polygonatum odoratum medicinal materials were purchased from Bozhou Yuanshengtang Pharmaceutical Co., Ltd. The medicinal materials used in the following examples were all the same batch products purchased from the company; the macroporous resins of various types were purchased from Tianjin Yunkai Resin Technology Co., Ltd.; the quercetin reference substance (batch number 100081-202010, content 99.8%) was purchased from China Institute for Drug Control; the 5,7,6'-trihydroxy-6,8-dimethyl-4'-methoxy-dihydrogen homoiso-flavone reference substance (batch number 20210601, content 99.4%) was a self-made reference substance; tert-butyl alcohol and ammonium sulfate were analytical pure; ethanol was pharmaceutical pure; and water was purified water.

[0035] ICR mice were purchased from Chengdu Dashuo Experimental Animal Co., Ltd.; Alanine aminotransferase and aspartate aminotransferase detection kits were purchased from Shanghai Zhiyan Biotechnology Co., Ltd.

[0036] High-pressure homogenizer: Model AH-MF1, AnTuoSi Nanotechnology (Suzhou) Co., Ltd.; HPLC liquid chromatograph: Shimadzu LC-20AT.

[0037] Example 1 Preparation and process screening of polygonatum odoratum alcohol extract

[0038] 1. High-pressure homogenization extraction

[0039] Polygonatum odoratum was mechanically pulverized and passed through a 60-mesh sieve, then extracted by high-pressure homogenization with ethanol. The extracted liquid was concentrated and dried, and the content of the index component quercetin and 5,7,6'-trihydroxy-6,8-dimethyl-4'-methoxy-dihydrogen high isoflavone (hereinafter referred to as dihydrogen high isoflavone) in the extract was determined by HPLC. Orthogonal test design was used to screen the ethanol concentration, material ratio and homogenization pressure. The factors and levels are shown in Table 1, and the orthogonal test results are shown in Table 2.

[0040] Table 1 Factor level table

[0041]

[0042] Table 2 Orthogonal test results

[0043]

[0044]

[0045] The results show that the preferred parameters for high-pressure homogenization extraction are: the extraction solvent is 70% ethanol, the material weight ratio of medicinal materials to ethanol is 1:10, and the high-pressure homogenization pressure is 400 bar.

[0046] 2. Purification process research

[0047] 2.1. Macroporous resin type screening

[0048] (1) The high-pressure homogenization extract of polygonatum odoratum was redispersed and dissolved with pure water to prepare a crude extract solution with a concentration of 8 mg / mL.

[0049] (2) Respectively take 7 different types of macroporous resin (AB-8, XAD-2, D101, D201, ADS-8, HPD-100, HP-10) 25g, add to 250mL conical flask, then add 100mL crude extract, constant temperature oscillation adsorption at room temperature for 12h, after adsorption, take supernatant to detect flavonoid index component quercetin and dihydrogen high isoflavone content and calculate the adsorption rate.

[0050] Adsorption rate = [(C0-C1) / C0]x100%, C0 is the quercetin and dihydrogen high isoflavone content in crude extract, C1 is the quercetin and dihydrogen high isoflavone content in supernatant.

[0051] (3) Results analysis: see table 3.

[0052] Table 3 adsorption rate of different types of macroporous resin

[0053] Type of macroporous resin Adsorption rate of quercetin (%) Adsorption rate of dihydrogen high isoflavone (%) AB-8 69.2 59.6 HP-10 71.5 47.3 D101 51.4 62.8 HPD-100 83.1 73.5 D201 45.3 65.4 XAD-2 65.2 43.2 ADS-8 77.4 68.1

[0054] According to the above results, considering the macroporous resin to quercetin and dihydrogen high isoflavone have high adsorption rate at the same time, HPD-100 type macroporous resin is the best type, the follow-up test uses HPD-100 type macroporous resin to further study the purification process.

[0055] 2.2 Purification process parameter screening

[0056] After determining the optimal macroporous resin type as HPD-100, on this basis, the concentration of crude extract and the column loading, the concentration and flow rate of eluent were screened. The crude extract was tested according to the parameters in table 6, after the liquid medicine was loaded, water was eluted to colorless, then ethanol was eluted, the eluate was collected, concentrated, dried, and the quercetin and dihydrogen high isoflavone content in the dry extract was detected, and the results were shown in table 4.

[0057] Table 4 purification process parameter screening results of macroporous resin

[0058]

[0059] According to the test results, the optimal purification process parameter condition was test 5, that is, the liquid concentration was 20mg / mL, the column loading was 5BV, the eluent concentration was 90% ethanol, and the eluent flow rate was 1BV / h.

[0060] Example 2 purification process screening of polygonatum odoratum water extract

[0061] We used purified water to extract the water extract from Polygonatum, and then used a three-phase extraction method to purify the water extract of Polygonatum, and the amount of tert-butyl alcohol and ammonium sulfate added in the purification process was screened. First, the Polygonatum was mechanically pulverized, passed through a 60 mesh sieve, 10 times the weight of the medicinal material (w / w) of purified water was added, and high pressure homogenization extraction was carried out (high pressure homogenization pressure was 400 bar), a total of 2 times, and the homogenization number of each extraction was 5 times. Then the medicinal liquid extracted twice was combined, vacuum reduced pressure concentrated to 1 / 50 of the original volume, different amounts of tert-butyl alcohol and ammonium sulfate were added according to the table below, stirred for 2 hours, and then static layering was carried out at 2-8°C, the lower water phase containing polysaccharide and ammonium sulfate was collected, and the water phase was ultrafiltered to remove ammonium sulfate. Then the water phase was freeze-dried, and the total polysaccharide content in the extract was detected.

[0062] 1) Ammonium sulfate addition amount screening: The volume of the concentrated extract of Polygonatum was fixed at 200ml, the amount of tert-butyl alcohol added was 200ml, and the total polysaccharide content was investigated when the amount of ammonium sulfate added was 10g, 20g, 30g, 40g, 50g.

[0063] Table 5 Ammonium sulfate addition amount screening

[0064] Test No. Amount of ammonium sulfate added (g) Total polysaccharide content (%) 1 10 55.4 2 20 58.9 3 30 65.7 4 40 71.8 5 50 68.6 6 60 62.4

[0065] As shown in the above table, when the amount of ammonium sulfate added increased from 10g to 40g, the total polysaccharide content in the extract was significantly improved. When the amount of ammonium sulfate added was further increased to 50g and 60g, the total polysaccharide content began to decrease. In summary, the optimal amount of ammonium sulfate added was 40g.

[0066] 2) Tert-butyl alcohol addition amount screening: The volume of the concentrated extract of Polygonatum was fixed at 200ml, the amount of ammonium sulfate added was 40g, and the total polysaccharide content was investigated when the amount of tert-butyl alcohol added was 100ml, 200ml, 300ml, 400ml, 500ml, 600ml.

[0067] Table 6 Tert-butyl alcohol addition amount screening

[0068] Test No. Amount of tert-butyl alcohol added (ml) Total polysaccharide content (%) 1 100 65.4 2 200 70.7 3 300 76.8 4 400 71.4 5 500 67.5 6 600 63.1

[0069] As shown in the results, when the amount of tert-butyl alcohol added increased from 100ml to 300ml, the total polysaccharide content in the extract was significantly improved. When the amount of tert-butyl alcohol added was further increased to more than 400ml, the total polysaccharide content began to decrease. In summary, the optimal amount of tert-butyl alcohol added was 300ml.

[0070] In summary, the preferred process parameters of the three-phase extraction method are: concentrated extract: ammonium sulfate: tert-butyl alcohol = 5:1:7.5 (ml / g / ml).

[0071] Comparative example

[0072] The Polygonatum multiflorum alcohol extract and water extract are prepared according to a conventional method known in the art, wherein the Polygonatum multiflorum is extracted by water decoction or reflux extraction, and the total polysaccharide is purified by alcohol precipitation, and the specific steps are as follows:

[0073] Preparation of the Polygonatum multiflorum alcohol extract: the Polygonatum multiflorum is mechanically pulverized and sieved through a 60-mesh sieve, then 70% ethanol is added at 10 times the weight of the Polygonatum multiflorum as an extraction solvent, and high-pressure homogenization extraction is performed under a pressure of 400 bar, for a total of 2 times, and the homogenization number is 4 times for each extraction, the extraction liquid is combined and concentrated and dried to obtain a crude extract. The crude extract is dissolved in purified water to prepare a 20 mg / ml crude extract solution, then an HPD-100 type macroporous resin column is used, and the column loading amount is 5 BV, after adsorption equilibrium, purified water is used for elution until colorless, then 90% ethanol is used for elution, the eluent flow rate is 1 BV / h, the 90% ethanol eluate is collected, concentrated and dried to obtain the purified Polygonatum multiflorum alcohol extract. The content of quercetin in the alcohol extract is 14.27 mg / g, and the content of dihydrogenated high isoflavone is 9.75 mg / g.

[0074] Preparation of the Polygonatum multiflorum water extract: the Polygonatum multiflorum is mechanically pulverized and sieved through a 60-mesh sieve, then water is added at 10 times the weight of the Polygonatum multiflorum for decoction extraction, for a total of 3 times, and each time is 2 hours, after the extraction is completed, the extraction liquid is first concentrated under reduced pressure, then ethanol is added to the concentrated extraction liquid to make the alcohol content of the liquid reach 50%, after stirring uniformly, it is left overnight, the supernatant is discarded, the precipitate is centrifuged and then freeze-dried to obtain the Polygonatum multiflorum water extract, and the total polysaccharide content is 47.6%.

[0075] Through the above comparative test, compared with the existing conventional method, the high-pressure homogenization extraction and three-phase extraction used in the present application can significantly improve the extraction efficiency and product purity of flavonoids and total polysaccharides.

[0076] Example 3 Preparation of Polygonatum multiflorum extract

[0077] According to the processes screened in Example 1 and Example 2, the alcohol extract and water extract of Polygonatum multiflorum are prepared respectively, and then mixed according to different proportions (as shown in Table 9) to obtain Polygonatum multiflorum extracts with different proportions, and the specific steps are as follows:

[0078] Preparation of the Polygonatum multiflorum alcohol extract: the Polygonatum multiflorum is mechanically pulverized and sieved through a 60-mesh sieve, then 70% ethanol is added at 10 times the weight of the Polygonatum multiflorum as an extraction solvent, and high-pressure homogenization extraction is performed under a pressure of 400 bar, for a total of 2 times, and the homogenization number is 4 times for each extraction, the extraction liquid is combined and concentrated and dried to obtain a crude extract. The crude extract is dissolved in purified water to prepare a 20 mg / ml crude extract solution, then an HPD-100 type macroporous resin column is used, and the column loading amount is 5 BV, after adsorption equilibrium, purified water is used for elution until colorless, then 90% ethanol is used for elution, the eluent flow rate is 1 BV / h, the 90% ethanol eluate is collected, concentrated and dried to obtain the purified Polygonatum multiflorum alcohol extract. The content of quercetin in the alcohol extract is 14.27 mg / g, and the content of dihydrogenated high isoflavone is 9.75 mg / g.

[0079] Rehmannia glutinosa water extract: First, Rehmannia glutinosa was mechanically pulverized and passed through a 60-mesh sieve. Purified water was added at 10 times the weight of the medicinal material, and high-pressure homogenization extraction was performed under a pressure of 400 bar. The extraction was performed twice, with 5 homogenization times for each extraction. The extract was combined and vacuum reduced pressure concentrated to 1 / 50 of the original volume. Then, according to the ratio of concentrated extract: ammonium sulfate: tert-butyl alcohol = 5: 1: 7.5 (ml / g / ml), tert-butyl alcohol and ammonium sulfate were added, stirred for 2 hours, and then allowed to stand at 2-8°C to separate the layers. The lower aqueous phase containing polysaccharides was collected, filtered to remove ammonium sulfate using an ultrafiltration membrane with a molecular weight cutoff of 10000, and freeze-dried to obtain the purified Rehmannia glutinosa water extract. The total polysaccharide content of the water extract was 78.5% by detection.

[0080] Different proportions of Rehmannia glutinosa extract were prepared according to the following table.

[0081] Table 7 Proportions of Rehmannia glutinosa extract

[0082] Sample No. Polygona japonica alcohol extract (g) Polygona japonica water extract (g) A 5 1 B 3 1 C 1 1 D 1 0 E 0 1

[0083] Example 4: Test study on the protective effect of Rehmannia glutinosa extract on acute gastric mucosal injury in mice induced by liquor and the effect of relieving alcoholism and protecting liver

[0084] After one week of adaptive feeding, ICR mice were randomly divided into 7 groups, namely normal group, model group, and Rehmannia glutinosa extract A (20 mg / kg), Rehmannia glutinosa extract B (20 mg / kg), and Rehmannia glutinosa extract C (20 mg / kg), Rehmannia glutinosa extract D (20 mg / kg), Rehmannia glutinosa extract E (20 mg / kg) groups, 10 in each group. Rehmannia glutinosa extract A / B / C / D / E was prepared according to the method in Example 3. Before the experiment, the mice in each group were weighed, labeled, and recorded. During the gavage, the body weight changes, food intake, water intake, mental state, and other general conditions of the mice were recorded. The normal group and the model group were gavaged with normal saline, and the Rehmannia glutinosa extract groups were gavaged with the corresponding Rehmannia glutinosa extract, for 7 consecutive days.

[0085] Modeling began on the 7th day, and each dosing group was normally gavaged with the corresponding Rehmannia glutinosa extract at the corresponding dose. The normal group and the model group were gavaged with normal saline at the corresponding dose. Two hours later, 52-degree liquor was administered at a dose of 10 ml / kg to all groups except the normal group.

[0086] After administration, the activity of the mice in each group was observed, and the number of drunk mice in each group and the recovery time from the loss of righting reflex to the recovery of righting reflex (i.e., the maintenance time of the drunk state) were recorded. The animals were sacrificed after 12 hours, and blood was taken for detection of glutamic-pyruvic transaminase and glutamic-oxalacetic transaminase. After washing the residual contents on the stomach with normal saline, the stomach was spread flat on the coordinate paper and fixed after being dried with filter paper. The gastric mucosal bleeding area and the corresponding total gastric tissue area were calculated by image processing software after photographing and recording. The gastric mucosal injury index was calculated according to the following formula.

[0087] Gastric mucosal injury index (%) = gastric mucosal bleeding area / total area of corresponding gastric tissue x 100

[0088] Table 8 Gastric mucosal injury index of mice in each group

[0089]

[0090]

[0091] Table 9 The drunken state maintenance time of mice in each group

[0092] Group Number of drunk in each group Drunk time (hours) Normal group 0 N / A Model group 10 7.23±1.61 Polygona japonica extract A 10 4.51±0.84 Polygona japonica extract B 7 2.18±0.72 Polygona japonica extract C 8 3.64±1.03 Polygona japonica extract D 9 5.35±0.92 Polygona japonica extract E 10 6.17±1.15

[0093] Table 10 The detection results of serum glutathione and glutathione transaminase in mice in each group

[0094] Group Alanine aminotransferase (U / L) Aspartate aminotransferase (U / L) Normal group 37±5 108±16 Model group 278±87 347±94 Polygona japonica extract A 157±74 204±65 Polygona japonica extract B 116±65 132±69 Polygona japonica extract C 101±92 183±76 Polygona japonica extract D 193±58 226±86 Polygona japonica extract E 224±83 285±71

[0095] Ethanol in wine and alcoholic beverages is an organic solvent, which has strong corrosive effect on gastric mucosa, can destroy the gastric mucosa surface mucus layer and mucus cells, and cause acute inflammation, resulting in phenomena such as congestion, edema, bleeding, erosion and ulcer. In this experiment, it was observed that ethanol caused rough bleeding strips and a large number of bleeding points in the gastric mucosa; at the same time, the results of gastric histopathology showed that 52-degree liquor could change the epithelial layer of the gastric mucosa, leading to a large number of epithelial cell shedding, and a large number of inflammatory cell infiltration. As shown in Table 8, compared with the normal group, the gastric mucosal injury index of the model group was significantly increased, and the polygonatum extract A / B / C / D / E group could reduce the gastric mucosal injury index to a certain extent, and the effect of polygonatum extract B was the best. As shown in Table 9, compared with the model group, the polygonatum extract A / B / C / D / E group could significantly shorten the maintenance time of the drunken state, and achieve the effect of alcoholism, and the effect of polygonatum extract B was the best. In addition, as shown in Table 10, compared with the model group, the polygonatum extract A / B / C / D / E group could reduce the serum glutathione and glutathione transaminase, indicating that it could effectively protect the liver.

[0096] In summary, the polygonatum extract prepared by the present application can shorten the drunken maintenance time of the drunken model mice, reduce the damage of alcohol to the gastric mucosa and liver of mice, and thus play a certain effect of alcoholism.

Claims

1. Use of a Polygonatum multiflorum extract in the preparation of an anti-alcoholic medicament or health food, characterized in that, The preparation method of the polygonatum extract includes the following steps: S1, crushing polygonatum officinale, then adding 60-90% ethanol for high-pressure homogenization extraction, after the extraction is completed, first concentrate and dry the extract, then dissolve with water and prepare 10-50 mg / ml of the crude extract, put the crude extract on a macroporous resin column, first elute with water until colorless, then elute with 60-90% ethanol as eluent, collect the ethanol eluate, concentrate and dry to obtain the polygonatum alcohol extract; S2, crushing polygonatum officinale, then adding water for high-pressure homogenization extraction, after the extraction is completed, first concentrate the extract, then add tert-butyl alcohol and ammonium sulfate to the concentrated solution, stir and then stand for layering, collect the lower aqueous phase containing polysaccharides, remove the ammonium sulfate and then freeze-dry to obtain the polygonatum water extract; S3, mix the polygonatum alcohol extract and the water extract according to a weight ratio of 1-5:1 to obtain a polygonatum extract rich in total flavonoids and total polysaccharides, wherein the total flavonoids include quercetin and dihydrogen high isoflavones.

2. Use according to claim 1, characterized in that: The pressure of the high-pressure homogenization extraction is 200-700 bar.

3. Use according to claim 1, characterized in that: The filler of the macroporous resin column is HPD-100 type macroporous resin.

4. The use according to claim 1, characterized in that: The column loading amount of the crude extract is 3-8 BV, and the eluent flow rate is 1-3 BV / h.

5. The use according to claim 1, characterized in that: The ratio of the concentrated solution to ammonium sulfate to tert-butyl alcohol is (3-8):(0.5-1.5):(5-10) ml / g / ml.

6. The use according to claim 1, characterized in that: The temperature of the standing and layering is 2-8℃.

7. The use according to claim 1, characterized in that: The polygonatum extract plays a role of alcoholism relief by inhibiting the damage of alcohol to the gastric mucosa and liver and shortening the maintenance time of drunken state.

8. The use according to claim 1, characterized in that: The mixing ratio of the polygonatum alcohol extract to the polygonatum water extract is 3:1.

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