一种从蜂花粉中分离得到的低分子多糖及其提取工艺

The extraction and purification of lotus bee pollen polysaccharides using microwave-assisted extraction addressed the lack of research on the bioactivity of lotus bee pollen polysaccharides. The obtained low-molecular-weight polysaccharide LBPP-1 showed significant effects in antioxidation, hypoglycemia, and anti-inflammation.

CN117327206BActive Publication Date: 2026-07-17ZHONGKAI UNIV OF AGRI & ENG

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
ZHONGKAI UNIV OF AGRI & ENG
Filing Date
2023-08-23
Publication Date
2026-07-17

AI Technical Summary

Technical Problem

Current research on the bioactivity of lotus bee pollen polysaccharides is relatively scarce, especially regarding their antioxidant, hypoglycemic, and anti-inflammatory properties, and there is a lack of effective extraction and purification processes.

Method used

Microwave-assisted extraction of lotus bee pollen polysaccharides was performed, combined with Sevag method for protein removal and DEAE-52 cellulose ion column chromatography. The extraction conditions were optimized to obtain low molecular weight polysaccharide LBPP-1, which is composed of arabinose, glucosamine hydrochloride, galactose and glucose, and has a β-configuration and triple helix structure.

Benefits of technology

Lotus bee pollen polysaccharide exhibits significant antioxidant capacity, scavenging DPPH, ABTS and hydroxyl free radicals, inhibiting AAPH-induced erythrocyte hemolysis, and possessing anti-inflammatory effects. In in vitro hypoglycemic experiments, it also showed inhibition of α-amylase and α-glucosidase activity.

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Abstract

本发明属于蜂花粉多糖分离的技术领域,具体涉及一种从蜂花粉中分离得到的低分子多糖及其提取工艺。采用微波辅助法提取粗多糖,再通过对荷花蜂花粉粗多糖进行Sevag法除蛋白后,采用DEAE‑52纤维素离子柱层析,得到纯化的低分子多糖LBPP‑1,其分子量为1625Da,主要由阿拉伯糖、盐酸氨基葡萄糖、半乳糖和葡萄糖组成,其结构中含有β‑构型糖苷键以及具有三螺旋结构,通过体外实验证明,荷花蜂花粉多糖具有清除DPPH、ABTS、羟基自由基的能力,且表现出较好的清除能力,此外,荷花蜂花粉多糖还可以抑制AAPH诱导的红细胞溶血,在细胞水平上表现出潜在的抗氧化能力。
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