A synergistic composition containing pqq and a method for preparing the same

Abstract

The present application relates to the technical field of agricultural technology, and provides a PQQ-containing synergistic composition and a preparation method thereof, the composition comprising the following components: PQQ, yeast extract, a cosolvent, a surfactant and water; the present application has the beneficial effects of effectively reducing the use amount of chemical fertilizers and pesticides, improving the use rate of chemical fertilizers and pesticides, prolonging the action time of the drugs, effectively increasing the yield and economic value of crops, and being a composition which is convenient to apply, safe and environmentally friendly, and low in price.

Description

Technical Field

[0001] This invention relates to the field of agricultural technology, specifically to a synergistic composition containing PQQ and its preparation method. Background Technology

[0002] With rapid economic development, the overuse of pesticides and fertilizers has seriously impacted the ecological environment, becoming a major obstacle to sustainable agricultural development. Reducing pesticide and fertilizer use has become an urgent need for agricultural production in my country. Currently, my country's pesticide and fertilizer usage is characterized by large volumes but extremely low utilization rates. This not only significantly reduces economic benefits but also increases pesticide resistance in insects and pathogens, as well as fertilizer tolerance in crops, making them highly susceptible to environmental pollution.

[0003] Focusing on crops that require large amounts of fertilizer and pesticides, such as fruits, vegetables, and tea, with particular emphasis on advantageous areas, core production areas, and brand bases, we will promote key technologies such as replacing chemical fertilizers with organic fertilizers and replacing chemical control with green pest control to reduce the amount of chemical fertilizers and pesticides used and improve their utilization rate.

[0004] Therefore, from the perspective of effectively reducing the amount of fertilizer and pesticide used and effectively improving the utilization rate of fertilizer and pesticide, a synergistic composition containing PQQ and its preparation method are proposed. Summary of the Invention

[0005] To address the aforementioned technical problems, this invention provides a synergistic composition containing PQQ and its preparation method. While ensuring increased crop yield and efficiency, it effectively reduces the amount of fertilizers and pesticides used, improves the utilization rate of fertilizers and pesticides, prolongs the action time of the drugs, and effectively increases crop yield and economic value. Moreover, it is a composition that is convenient to apply, safe, environmentally friendly, and inexpensive.

[0006] The objective of this invention is achieved through the following technical solution:

[0007] One aspect of the present invention provides a synergistic composition containing PQQ, comprising the following components: PQQ, yeast extract, cosolvent, surfactant, and water.

[0008] Furthermore, in one specific embodiment of this solution, a synergistic composition containing PQQ comprises the following components by weight percentage: 0.05-5% PQQ, 1-15% yeast extract, 0.005-2% cosolvent, 1-10% surfactant, and water as the balance.

[0009] PQQ, scientifically known as pyrroloquinoline quinone, is referred to by the world medical community as the fourteenth vitamin. First discovered in 1964, it possesses strong redox activity and is a complex that can bind to metals to exert its biological activity in the body. Furthermore, PQQ participates in various redox reactions, mediating a series of cellular and molecular biochemical reactions as a coenzyme, thereby influencing physiological and metabolic processes.

[0010] PQQ is widely found in nature, including in many animals, plants, and microorganisms. It is also present in some common foods, such as beans, wood ear mushrooms, broccoli, and grapefruit. Currently, there are two main sources: chemical synthesis and extraction through fermentation using filamentous microorganisms.

[0011] In one specific embodiment of this solution, the PQQ is obtained through fermentation by raw silk microorganisms.

[0012] In one specific embodiment of this solution, the yeast extract is bread yeast extract.

[0013] Yeast extract is a brownish-yellow soluble paste (yeast extract) made from fresh yeast emulsion using modern high-tech methods such as autolysis, enzymatic hydrolysis, separation, and concentration, in accordance with the provisions of the Chinese Pharmacopoeia. Yeast extracts can be categorized by their raw material source into baker's yeast extract, brewer's yeast (a byproduct of beer brewing), and Candida albicans. Among these, yeast extracts produced from pure cultured baker's yeast are the cleanest and safest, meeting food and drug certification standards.

[0014] In one specific embodiment of this solution, the co-solvent is one or both of ethanolamine and potassium hydroxide.

[0015] In one specific embodiment of this solution, the surfactant is one or both of sorbitan monolaurate and polyoxyethylene sorbitan monooleate.

[0016] In one specific embodiment of this solution, a synergistic composition containing PQQ is provided, wherein the composition is a liquid formulation.

[0017] On the other hand, the present invention also provides a preparation method for preparing a synergistic composition containing PQQ, the method comprising the following steps:

[0018] S1. Add the yeast extract and the first portion of water to the mixing vessel, heat to 60-80°C, stir until completely dissolved, and the solution becomes a transparent solution, then cool to room temperature;

[0019] S2. Dissolve PQQ and co-solvent in the second batch of water, then slowly add the mixture to the mixing vessel and stir.

[0020] S3. Slowly add the remaining water into the mixing vessel and continue stirring for 30-60 minutes to obtain the synergistic composition containing PQQ.

[0021] In one specific embodiment of this scheme, the stirring and mixing time in step S1 is 30 to 60 minutes.

[0022] The present invention also provides a method of using a synergistic composition containing PQQ, the method comprising mixing the composition with a fertilizer or pesticide and applying it throughout the entire growth cycle of the crop.

[0023] In this invention, it should be noted that PQQ, acting as a coenzyme, mediates a series of cellular and molecular biochemical reactions, inducing root growth in plants, regulating the ratio of male to female flowers, and promoting the absorption and utilization of nutrients. Simultaneously, through its superior antioxidant capacity, it can effectively scavenge H2O. - and O 2- This avoids the rapid oxidation and decomposition of pesticides and fertilizers, prolongs the inactivation time of pesticides and fertilizers on target crops, and achieves the reduction and sustained effect of pesticides and fertilizers, thereby enhancing their efficacy.

[0024] Yeast extract is rich in complete protein, balanced essential amino acids, B complex vitamins, nucleotides, trace elements, growth factors, etc. It can rapidly promote plant cell division and differentiation, promote the rapid absorption and utilization of nutrients by plants, effectively improve the utilization rate of drugs caused by the gradual decay caused by factors such as light and oxidation, and at the same time provide the necessary energy for the biochemical reaction of PQQ, thus synergistically achieving the reduction and sustained effect of pesticides and fertilizers.

[0025] The beneficial effects of this invention are:

[0026] (1) Through the synergistic effect of PQQ and yeast extract, plant cells are induced to divide and differentiate, promoting the growth of plant roots and plants. As pesticides and fertilizers gradually decay over time due to factors such as light, oxidation, and leaching, the absorption and utilization of pesticides and fertilizers by plants are rapidly promoted, thereby increasing the absorption rate of pesticides and fertilizers and playing a role in reducing dosage and increasing efficiency.

[0027] (2) PQQ's super antioxidant capacity can effectively remove HO from plants. - and O 2- This slows down the decay rate of pesticides and fertilizers, and prolongs their effect on target crops, thereby reducing the amount used and increasing the efficiency.

[0028] (3) The composition prepared in this scheme is a liquid preparation, which is convenient to use, and the raw materials are safe, environmentally friendly and inexpensive. Attached Figure Description

[0029] Figure 1 The effects of different seed soaking treatments on soybeans;

[0030] Figure 2 The effects of different seed soaking treatments on wheat. Detailed Implementation

[0031] The technical solution of the present invention will be further described in detail below with reference to the accompanying drawings, but the scope of protection of the present invention is not limited to the following description. Unless otherwise specified in the embodiments, conventional conditions or conditions recommended by the manufacturer shall apply. Reagents or instruments used that do not specify the manufacturer are all commercially available conventional products.

[0032] Example 1

[0033] Example 1 provides a synergistic composition containing PQQ, comprising, by weight percentage: 0.05% PQQ, 1% yeast extract, 0.005% ethanolamine, 1% sorbitol monolaurate, and the balance being water.

[0034] A method for preparing a synergistic composition containing PQQ includes the following steps:

[0035] Step 1: Add the yeast extract and the first portion of water to the mixing vessel, heat to 60-80℃, stir for 30-60 minutes until completely dissolved and transparent, then cool to room temperature;

[0036] Step 2: Dissolve PQQ and co-solvent in the second batch of water, then slowly add the mixture to the mixing vessel and stir.

[0037] Step 3: Slowly add the remaining water into the mixing vessel and continue stirring for 30-60 minutes to obtain the synergistic composition containing PQQ.

[0038] Example 2

[0039] Example 2 provides a synergistic composition containing PQQ, comprising, by weight percentage: 0.1% PQQ, 2% yeast extract, 0.005% ethanolamine, 0.001% potassium hydroxide, 1% dehydrated sorbitan monolaurate, 0.5% polyoxyethylene dehydrated sorbitan monooleate, and water as the balance.

[0040] The preparation method in this embodiment is the same as that in Example 1.

[0041] Example 3

[0042] Example 3 provides a synergistic composition containing PQQ, comprising, by weight percentage: 0.5% PQQ, 3% yeast extract, 0.01% ethanolamine, 0.001% potassium hydroxide, 1.5% dehydrated sorbitan monolaurate, 0.5% polyoxyethylene dehydrated sorbitan monooleate, and water as the balance.

[0043] The preparation method in this embodiment is the same as that in Example 1.

[0044] Example 4

[0045] Example 4 provides a synergistic composition containing PQQ, comprising, by weight percentage: 1% PQQ, 5% yeast extract, 0.02% ethanolamine, 2.5% sorbitan monolaurate, 0.5% polyoxyethylene sorbitan monooleate, and the balance being water.

[0046] The preparation method in this embodiment is the same as that in Example 1.

[0047] Example 5

[0048] Example 5 provides a synergistic composition containing PQQ, comprising, by weight percentage: 1.5% PQQ, 5.5% yeast extract, 0.022% potassium hydroxide, 2.5% sorbitan monolaurate, 1.5% polyoxyethylene sorbitan monooleate, and the balance being water.

[0049] The preparation method in this embodiment is the same as that in Example 1.

[0050] Example 6

[0051] Example 6 provides a synergistic composition containing PQQ, comprising, by weight percentage: 2% PQQ, 7% yeast extract, 0.05% ethanolamine, 4.5% polyoxyethylene sorbitan monooleate, and the balance being water.

[0052] The preparation method in this embodiment is the same as that in Example 1.

[0053] Example 7

[0054] Example 7 provides a synergistic composition containing PQQ, comprising, by weight percentage: 2.5% PQQ, 8% yeast extract, 0.15% ethanolamine, 5% polyoxyethylene sorbitan monooleate, and the balance being water.

[0055] The preparation method in this embodiment is the same as that in Example 1.

[0056] Example 8

[0057] Example 8 provides a synergistic composition containing PQQ, comprising, by weight percentage: 3% PQQ, 10% yeast extract, 0.5% ethanolamine, 5% polyoxyethylene dehydrated sorbitan monooleate, 0.5% dehydrated sorbitan monolaurate, and water as the balance.

[0058] The preparation method in this embodiment is the same as that in Example 1.

[0059] Example 9

[0060] Example 9 provides a synergistic composition containing PQQ, comprising, by weight percentage: 4% PQQ, 11% yeast extract, 1% ethanolamine, 5% polyoxyethylene dehydrated sorbitan monooleate, 1.5% dehydrated sorbitan monolaurate, and the balance being water.

[0061] The preparation method in this embodiment is the same as that in Example 1.

[0062] Example 10

[0063] Example 10 provides a synergistic composition containing PQQ, comprising, by weight percentage: 4.5% PQQ, 12% yeast extract, 1.5% ethanolamine, 6% polyoxyethylene dehydrated sorbitan monooleate, 1.5% dehydrated sorbitan monolaurate, and water as the balance.

[0064] The preparation method in this embodiment is the same as that in Example 1.

[0065] Example 11

[0066] Example 11 provides a synergistic composition containing PQQ, comprising, by weight percentage: 4.8% PQQ, 14% yeast extract, 1.6% ethanolamine, 6% polyoxyethylene dehydrated sorbitan monooleate, 3% dehydrated sorbitan monolaurate, and water as the balance.

[0067] The preparation method in this embodiment is the same as that in Example 1.

[0068] Example 12

[0069] Example 12 provides a synergistic composition containing PQQ, comprising, by weight percentage: 5% PQQ, 15% yeast extract, 1.6% ethanolamine, 6% polyoxyethylene dehydrated sorbitan monooleate, 3% dehydrated sorbitan monolaurate, and the balance being water.

[0070] The preparation method in this embodiment is the same as that in Example 1.

[0071] Test Example 1

[0072] The experiment was conducted indoors on soybeans and wheat, and a reduced-dosage pesticide soaking test was performed on each.

[0073] In the indoor experiment, 20 soybean seeds and 50 wheat seeds of uniform size were selected for each treatment and soaked in diluted solution for 12 hours. After rinsing, they were placed in germination boxes and then in a light incubator at a controlled temperature of 25±2℃ for 5 days. Germinated and rooted plants were then photographed for comparison, and root length, stem length, and root quantity were compared. A total of 5 treatments (AE) were selected in the experiment. The control group (CK) was 0.2 ppm indolebutyric acid diluted solution, and the other treatments were compared with the CK control by adding other agents.

[0074] The five comparative examples are as follows: A: CK control; B: Example 2 diluted 2000 times (PQQ 0.5 ppm + yeast extract 10 ppm); C: 0.1% PQQ solution diluted 2000 times (PQQ 0.5 ppm); D: 2% yeast extract solution diluted 2000 times (yeast extract 10 ppm); E: CK dosage reduced to 50% of the original concentration + Example 2 diluted 2000 times (PQQ 0.5 ppm + yeast extract 10 ppm). The preparation methods for comparative examples C and D are the same as in Example 2, except that one of the active ingredients is removed.

[0075] Experimental results:

[0076] The effects of different seed soaking treatments on soybeans are shown in the figure. Figure 1 The effects of different seed soaking treatments on wheat are shown in [reference needed]. Figure 2 .

[0077] from Figure 1 , Figure 2 It can be seen that the root and stem lengths of soybeans treated with treatment B are significantly better than those of other soybean treatment groups, and the root and stem lengths of wheat treated with treatment B are also significantly better than those of other wheat treatment groups. Therefore, the composition prepared in this invention (treatment B) can significantly increase the root and stem lengths of soybeans and wheat; and from Figure 1 As can be seen, the amount of lateral roots of soybeans after treatment B is significantly greater than that after treatments in other groups. Therefore, the composition prepared in this invention can increase the amount of lateral roots of soybeans.

[0078] Therefore, through experimental comparison of soybeans and wheat, both wheat and soybeans treated in treatment group B showed significant differences compared to other treatment groups.

[0079] Furthermore, the growth of soybeans after treatment A and treatment E, as well as the growth of wheat after treatment A and treatment E, show that even with a 50% reduction in the dosage, the composition prepared in this invention (treatment E) can achieve the same effect as the control group (CK), thus achieving the effect of reducing the dosage while increasing the efficacy.

[0080] Test Example 2

[0081] The experiment was conducted on grapes and leafy vegetables in the field, and the reduction of pesticide and fertilizer dosages was tested separately.

[0082] Field trials were conducted in grape industrial parks in Dayi County and Qionglai City, Sichuan Province, respectively, on the Early Summer and Sunshine Rose varieties. Fifty-four grapevines of each variety with consistent growth were selected from the industrial parks, resulting in five treatments (FJ). The control (CK) was compared to the standard treatment plus chlorpyrifos and gibberellic acid. During the fruit setting stage, the chlorpyrifos treatment was 2 ppm + gibberellic acid 10 ppm; during the fruit expansion stage, the chlorpyrifos treatment was 5 ppm + gibberellic acid 25 ppm. Other treatments were compared to the CK control with additional pesticides.

[0083] The comparative experiment was repeated three times, randomly arranged, with five comparative examples as follows: F: CK control; G: Example 2 diluted 200 times (PQQ 5ppm + yeast extract 100ppm); H: 0.1% PQQ solution diluted 200 times (PQQ 5ppm); I: 2% yeast extract solution diluted 200 times (yeast extract 100ppm); J: CK dosage reduced to 80% of the original concentration + Example 2 diluted 200 times (PQQ 5ppm + yeast extract 100ppm). The preparation methods for comparative examples H and I were the same as in Example 2, except that one of the active ingredients was removed.

[0084] The above comparative experiment was repeated three times, with the following treatment periods for the three varieties:

[0085] Sunshine Rose: Fruit settling occurs on April 26th, and fruit expansion occurs on May 6th.

[0086] Early summer: Fruit setting is on April 3rd, and fruit expansion is on April 15th.

[0087] When applying the pesticide, the fruit load on each tree was recorded, and the pesticide was applied by soaking the fruit in water for each treatment. At harvest, the weight of the single fruit and the yield increase were examined.

[0088] Experimental results:

[0089] 1. Effects of different pesticide treatments on different grape varieties in Dayi County, Sichuan Province (see Table 1)

[0090] Table 1. Effects of different pesticide treatments on different grape varieties (2023)

[0091]

[0092]

[0093] 2. Effects of different pesticide treatments on different grape varieties in Qionglai City, Sichuan Province (see Table 2)

[0094] Table 2 Effects of different pesticide treatments on different grape varieties (2023)

[0095]

[0096] As can be seen from Tables 1 and 2, the composition prepared by this invention (treatment G) can significantly increase the average single fruit weight of grapes and increase grape yield. Through experimental comparison in two locations, grapes treated with treatment group G in both locations showed significant differences compared with grapes treated with other treatment groups. Furthermore, the corresponding comparison between treatment F and treatment J shows that even with a 20% reduction in pesticide dosage, the composition prepared by this invention (treatment J) can still achieve the desired effect, achieving the effect of reducing pesticide use while increasing efficacy. Moreover, it is convenient to use, ensuring the economic benefits for users by reducing pesticide dosage, and is safe and environmentally friendly.

[0097] The second field experiment was conducted on Chinese cabbage in greenhouses within the Xinjin District Vegetable Industrial Park in Sichuan Province. Three greenhouses (each with an area of ​​approximately 260 m²) were selected within the industrial park. 2 Each greenhouse is divided into 6 areas, and 20m² of bok choy with consistent growth is selected from each area. 2 Six treatments were included in the study. Farmers in all six treatments routinely applied calcium-magnesium fertilizer (product technical indicators: calcium ≥65g / L, magnesium ≥75g / L, nitrogen ≥80g / L, boron ≥3g / L, zinc ≥10g / L), applied twice during the growing season with a 14-day interval. Data were converted to average yield per acre for comparison. Two experiments were conducted in December 2022 and June 2023.

[0098] Three greenhouses were used in a repeated comparative experiment three times, randomly arranged. The six comparative examples were: K: CK (clear water control); L: User-prescribed calcium-magnesium fertilizer control (product technical indicators: calcium ≥65g / L, magnesium ≥75g / L, nitrogen ≥80g / L, boron ≥3g / L, zinc ≥10g / L) diluted 500 times; M: User-prescribed calcium-magnesium fertilizer (product technical indicators: calcium ≥65g / L, magnesium ≥75g / L, nitrogen ≥80g / L, boron ≥3g / L, zinc ≥10g / L) diluted 500 times + the above Example 2 diluted 500 times (PQQ 2ppm + yeast extract 40ppm) mixed in a tank; N: User-prescribed calcium-magnesium fertilizer (product technical indicators: calcium ≥65g / L, magnesium ≥75g / L, nitrogen ≥80g / L, boron ≥3g / L, zinc ≥10g / L) diluted 500 times + 0.1% PQQ solution diluted 500 times (PQQ... 2ppm) mixed in a tank; O: User's conventional calcium-magnesium fertilizer (product technical indicators: calcium ≥65g / L, magnesium ≥75g / L, nitrogen ≥80g / L, boron ≥3g / L, zinc ≥10g / L) diluted 500 times + 2% yeast extract solution diluted 500 times (yeast extract 40ppm) mixed in a tank; P: User's conventional calcium-magnesium fertilizer (product technical indicators: calcium ≥65g / L, magnesium ≥75g / L, nitrogen ≥80g / L, boron ≥3g / L, zinc ≥10g / L) diluted 750 times + the above Example 2 diluted 500 times (PQQ 2ppm + yeast extract 40ppm) mixed in a tank. The preparation methods of Comparative Example M and Comparative Example N are the same as in Example 2, except that one of the active ingredients is removed.

[0099] 3. Effects of different pesticide treatments on the yield of Chinese cabbage in Xinjin District, Sichuan Province (see Table 2)

[0100] Table 3 Effects of different pesticide treatments on Chinese cabbage

[0101]

[0102] As can be seen from Table 3, the composition prepared by this invention (treatment M) can significantly increase the average yield of Chinese cabbage per mu (unit of land area). Through the comparison of two experiments in winter and summer, the Chinese cabbage treated with treatment group M showed significant differences compared with the Chinese cabbage treated with other treatment groups. Moreover, when reducing the amount of fertilizer used, the composition prepared by this invention (treatment P) can also achieve the ideal effect of treatment L, thus achieving the effect of reducing fertilizer use and increasing efficiency. Furthermore, it is convenient to use, can reduce the amount of fertilizer used, ensure the economic benefits of users, and is safe and environmentally friendly.

[0103] The above description is merely a preferred embodiment of the present invention. It should be understood that the present invention is not limited to the forms disclosed herein and should not be construed as excluding other embodiments. It can be used in various other combinations, modifications, and environments, and can be altered within the scope of the concept described herein through the above teachings or related technologies or knowledge. Modifications and variations made by those skilled in the art that do not depart from the spirit and scope of the present invention should be within the protection scope of the appended claims.

Claims

1. A synergistic composition containing PQQ, characterized in that, Included by weight percentage: PQQ 0.1%, yeast extract 2%, ethanolamine 0.005%, potassium hydroxide 0.001%, sorbitan monolaurate 1%, polyoxyethylene sorbitan monooleate 0.5%, water balance.

2. The synergistic composition containing PQQ according to claim 1, wherein the PQQ is obtained by fermentation with raw silk microorganisms.

3. The synergistic composition containing PQQ according to claim 1, characterized in that: the yeast extract is baker's yeast extract.

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