A fully automated PCR capillary assembly device and its assembly method

Abstract

The application discloses a kind of PCR capillary full-automatic assembly equipment and its assembly method;The equipment includes rack, work position switching mechanism, and with the different work position of work position switching mechanism respectively position corresponding pipe body feeding mechanism, sample addition connector feeding mechanism, hot-press docking mechanism, leak detection mechanism, cover body feeding mechanism and unloading mechanism.The work position switching mechanism is provided with a plurality of capillary fixtures;Each capillary fixture can cyclically move and sequentially pass through eight work positions.Capillary fixture is used to provide positioning and support for capillary body and sample addition connector.The application sequentially passes through pipe body feeding work position, sample addition connector feeding work position, hot-press docking work position, leak detection work position, defective piece rejection work position, replenishment work position, cover body feeding work position and unloading work position by capillary fixture, can automatically complete the assembly of PCR capillary, leak detection, capping operation, realizes the full-automatic assembly of PCR capillary with quality inspection process.

Description

Technical Field

[0001] This invention belongs to the field of capillary assembly technology, specifically relating to a fully automatic glass capillary assembly device and its assembly method. Background Technology

[0002] A PCR capillary is a small tube used for micro-PCR (Polymerase Chain Reaction) reactions. It is suitable for amplification reactions of small samples, saving reagents and sample volume and reducing waste generation.

[0003] PCR capillary tubes consist of the capillary body, sample delivery connector, and cap. Current assembly methods mostly involve manual heating with a hot air gun followed by compression, which is time-consuming, slow, results in uneven compression pressure, poor overall height stability, and poses a risk of leakage. Therefore, developing a fully automated glass capillary tube assembly device to improve assembly efficiency and quality is of great significance for meeting market demands and promoting the development of related industries. Summary of the Invention

[0004] The purpose of this invention is to provide a fully automated PCR capillary assembly device and its assembly method.

[0005] This invention provides a fully automated PCR capillary assembly device, comprising a frame, a station switching mechanism, and tube loading mechanisms, sample connector loading mechanisms, thermocompression mechanisms, leak detection mechanisms, cap loading mechanisms, and unloading mechanisms corresponding to different stations of the station switching mechanism. The station switching mechanism is equipped with multiple capillary fixtures; each capillary fixture can move cyclically and sequentially through eight stations. The capillary fixtures provide positioning and support for the capillary body and sample connector.

[0006] The tube feeding mechanism is used to place the capillary body onto the capillary fixture;

[0007] The sample feeding mechanism is used to place the sample feeding connector onto the capillary fixture, and to align and contact the sample feeding connector with the opening of the capillary body.

[0008] The aforementioned thermo-pressing docking mechanism is used to heat the connection between the capillary body and the sample dispensing connector, and to apply axial compressive force to the capillary body and the sample dispensing connector, so that the capillary body and the sample dispensing connector are inserted together to form a PCR capillary.

[0009] The leak detection mechanism determines whether there is a leak in the PCR capillary by pressurizing the opening of the sample loading connector in the PCR capillary obtained by hot pressing and observing whether there is gas leakage at the connection between the capillary body and the sample loading connector.

[0010] The aforementioned cap feeding mechanism is used to install the cap onto the sample loading connector of the PCR capillary.

[0011] The feeding mechanism is used to remove the PCR capillary tube with the cover attached from the capillary fixture.

[0012] Preferably, the capillary fixture includes a capillary mounting block, a lifting support plate, and a limiting block. The lifting support plate is slidably connected below the capillary mounting block. The capillary mounting block is provided with a hot-press mounting position and a cap mounting position; each hot-press mounting position is located directly above the lifting support plate. Both the hot-press mounting position and the cap mounting position include an upper connector positioning hole section and a lower tube body positioning hole section. A hot air channel groove is provided on the side of the capillary mounting block to cut off the hot-press mounting position. During the hot-press connection process, the connection between the sample feeding connector and the capillary body is exposed in the hot air channel groove; the cap mounting position is provided with a stepped surface to support the sample feeding connector.

[0013] Preferably, the workstation switching mechanism includes a circular track, a capillary fixture, a fixture positioning assembly, and a cyclic drive mechanism. The circular track is fixed to the frame; multiple sliding plates are arranged sequentially on the circular track and can circulate along the circular track under the drive of the cyclic drive mechanism. The capillary fixture is mounted on the sliding plates.

[0014] Preferably, a spring is provided between the lifting support plate and the capillary mounting block.

[0015] Preferably, each station has a corresponding fixture positioning assembly. The fixture positioning assembly is used to position the capillary fixture. The fixture positioning assembly includes a positioning fork and a telescopic drive assembly. Positioning rollers are mounted on the capillary fixture. The positioning fork is slidably connected to the frame in the vertical direction, and the telescopic drive assembly slides by a drive. A positioning groove is formed at the top of the positioning fork. The top opening of the positioning groove has a chamfer. When a capillary fixture is in a station, the positioning roller on the capillary fixture is aligned with the positioning fork on the fixture positioning assembly corresponding to that station. By extending the positioning fork, the positioning roller is engaged in the positioning groove.

[0016] Preferably, the tube feeding mechanism includes a tube feeding base, and a tube arrangement output assembly, a rotation and orientation assembly, and a tube transfer assembly mounted on the tube feeding base. The tube feeding base is fixed to the frame. The tube arrangement output assembly arranges and outputs the stacked capillary bodies one by one through a tube vibrating plate and a tube straight vibrating machine.

[0017] The aforementioned rotary orientation assembly is used to adjust the capillary body output by the tube arrangement output assembly to an upright, vertically facing position. It includes a rotating disk, a rotary drive mechanism, and a tube detection sensor. The rotating disk is rotatably connected to the tube loading base via a rotating shaft and is driven by the rotary drive mechanism. Multiple capillary insertion holes are sequentially arranged along the outer circumference of the rotating disk. These capillary insertion holes can rotate to align with the output port of the tube arrangement output assembly. Multiple capillary detection holes, corresponding one-to-one with each capillary insertion hole, are provided on the end face of the rotating disk. The capillary detection holes communicate with their corresponding capillary insertion holes. The tube detection sensor is an infrared sensor, fixed to the tube loading base, and facing the corresponding rotating disk. When a capillary insertion hole is aligned with the output port of the tube arrangement output assembly, the corresponding capillary detection hole aligns with the detection section of the tube detection sensor.

[0018] The tube transfer assembly is used to clamp the capillary body on the rotary orientation assembly and clamp it onto the capillary fixture.

[0019] Preferably, the hot-pressing docking mechanism includes a hot-pressing bracket, an upper pressing assembly, a lower pressing assembly, and a heating assembly. The hot-pressing bracket is fixed to the frame. The upper pressing assembly and the lower pressing assembly are respectively positioned directly above and below the corresponding workstations. The upper pressing assembly includes an upper pressing plate and an upper pressing cylinder. The lower pressing assembly includes a lower pressing plate and a lower pressing cylinder. The upper pressing cylinder and the lower pressing cylinder are fixed to the hot-pressing bracket. The push rod of the upper pressing cylinder is positioned downwards and is fixed to the upper pressing plate. The push rod of the lower pressing cylinder is positioned upwards and is fixed to the lower pressing plate. The heating assembly includes hot air guns. The air outlet of each hot air gun faces the hot air channel groove of the capillary fixture at the corresponding workstation. During the hot-pressing process, the hot air blown out by each hot air gun heats the connection between the capillary body and the sample dispensing connector. The upper pressing plate presses down on the sample dispensing connector, and the lower pressing plate presses up on the bottom lifting support plate of the capillary fixture. The lifting support plate pushes the capillary body up, causing the capillary body to be inserted into the sample dispensing connector.

[0020] Preferably, the leak detection mechanism includes a leak detection bracket, a sealing block, a leak detection air tube, a leak detection transfer assembly, a leak detection mounting block, a lifting and pressurizing assembly, a water tank, and a camera. The leak detection bracket is fixed to the frame. The leak detection mounting block, water tank, and camera are all fixed to the leak detection bracket. The top of the water tank is open; the camera is located directly above the water tank and facing downwards. The camera is used to capture images of the water tank and transmit them to a host computer to determine whether air bubbles appear in the water tank.

[0021] A sealing block is embedded and fixed in the leak detection mounting block; a leak detection hole is provided on the sealing block. The leak detection hole is a through hole with a diameter larger than that of the capillary tube; the PCR capillary tube formed by heat pressing can be inserted into the leak detection hole and supported by the top surface of the sealing block. The bottom end of the leak detection hole is connected to the water tank through a leak detection gas tube. The lifting and pressurizing assembly includes a lifting and pressurizing cylinder, a pressurizing mounting plate, and a pressurizing connector. The lifting and pressurizing cylinder is fixed on the leak detection bracket. The push rod of the lifting and pressurizing cylinder faces directly downward and is fixed with the pressurizing mounting plate; a pressurizing connector is fixed on the pressurizing mounting plate. The pressurizing connector is located directly above the leak detection hole. The pressurizing connector is connected to a gas source through a gas supply pipe. The leak detection transfer assembly is used to transfer the PCR capillary tube on the corresponding capillary fixture to the leak detection hole, and to put the PCR capillary tube back into the capillary fixture after the leak detection is completed.

[0022] Preferably, the fully automated PCR capillary assembly equipment also includes a leak removal mechanism and a replenishment mechanism. Along the movement direction of the capillary fixture, the leak removal mechanism and the replenishment mechanism are sequentially arranged between the leak detection mechanism and the cover feeding mechanism. The leak removal mechanism is used to remove PCR capillaries that are detected as leaking by the leak detection mechanism. The replenishment mechanism is used to place non-leaking PCR capillaries into the vacated positions on the capillary fixture created by the leak removal mechanism. The leak removal mechanism includes a removal support, a waste container, and a removal transfer assembly. The removal support is fixed to the frame; the waste container is fixed to the removal support and has an open top. The removal transfer assembly is used to transfer PCR capillaries identified as leaking on the capillary fixture at the leak removal station to the waste container.

[0023] The feeding mechanism includes a feeding support, a feeding assembly, and a feeding transfer assembly. The feeding support is fixed to the frame; the feeding assembly includes a feeding traverse assembly and a feeding seat. The feeding traverse assembly is a single-degree-of-freedom linear module; the feeding seat is mounted on the feeding traverse assembly. The feeding seat is used to hold a tray containing matrix-arranged PCR capillary tubes. The feeding transfer assembly is used to transfer the PCR capillary tubes from the feeding seat to the capillary fixture, and to transfer the PCR capillary tubes from the thermo-press mounting position of the capillary fixture to the cap mounting position.

[0024] Preferably, the cover feeding mechanism includes a cover support, and a cover arrangement output assembly and a cover transfer and installation assembly mounted on the cover support. The cover support is fixed to the frame. The cover arrangement output assembly outputs covers one by one through a cover vibrating plate and a cover direct vibrator; the cover transfer and installation assembly is used to pick up the covers output by the cover direct vibrator and clamp them onto the capillary fixture at the cover feeding station, pressing them into the top of the PCR capillary.

[0025] Preferably, the feeding mechanism includes a feeding bracket, and a receiving assembly, a first feeding transfer assembly, a second feeding transfer assembly, and a tray in / out assembly mounted on the feeding bracket. The receiving assembly includes a receiving seat and a receiving transverse movement assembly. The receiving transverse movement assembly is a linear module; the receiving seat is mounted on the receiving transverse movement assembly. The receiving seat is used to hold an empty tray. The first feeding transfer assembly is used to transfer PCR capillaries from the capillary fixture at the corresponding station to the receiving assembly.

[0026] The tray loading / unloading assembly is used to provide empty trays to the receiving assembly and to receive trays filled with PCR capillaries. The tray loading / unloading assembly includes a tray storage seat, a tray traversing assembly, an empty tray supply assembly, and a full tray output assembly. The tray storage seat is slidably connected to the unloading support and is driven by the tray traversing assembly.

[0027] An empty tray supply assembly and a full tray output assembly are mounted side-by-side on a tray storage base. The empty tray supply assembly includes a tray fixture and an empty tray ejection assembly. The tray fixture is fixed to the tray storage base and can stack multiple empty trays. The empty tray ejection assembly ejects the bottom empty tray from the tray fixture. The full tray output assembly includes a full tray output plate and a tray transfer assembly. The full tray output plate is slidably connected to the tray storage base and is driven to move by the tray transfer assembly. A second unloading and transfer assembly works in conjunction with the tray traversing assembly to transfer empty trays from the empty tray supply assembly to the receiving seat of the receiving assembly, and to transfer full trays from the receiving seat of the receiving assembly to the full tray output plate of the full tray output assembly.

[0028] The assembly method of this fully automated PCR capillary assembly device includes the following steps:

[0029] Step 1: The capillary fixtures on the station switching mechanism circulate in sequence, passing through the tube loading station, sample connector loading station, hot pressing and docking station, leak detection station, leaking part rejection station, replenishment station, cover loading station, and unloading station.

[0030] Step 2: When a capillary fixture arrives at the tube loading station, the tube loading mechanism outputs the capillary bodies one by one and places them vertically on the hot-press mounting position on the capillary fixture.

[0031] Step 3: When a capillary fixture arrives at the sample feeding station, the sample feeding mechanism outputs the sample feeding connectors one by one and places them vertically on the hot-press mounting position on the capillary fixture.

[0032] Step 4: When a capillary fixture arrives at the thermocompression docking station, the thermocompression docking mechanism applies pressure and squeezes the sample dispensing connector, so that the bottom opening of the sample dispensing connector is inserted and fixed together with the top opening of the capillary body to form a PCR capillary.

[0033] Step 4: When a capillary fixture arrives at the leak detection station, the leak detection mechanism pressurizes the inner lumen of the PCR capillary to determine whether the PCR capillary is leaking.

[0034] Step 5: When a capillary fixture arrives at the leak removal station, the leak removal mechanism removes the leaking PCR capillary into the waste bin based on the detection results of the PCR capillary at the leak detection station.

[0035] Step 6: When a capillary fixture arrives at the replenishment station, the replenishment mechanism places a pre-prepared uncapped PCR capillary into the gap created by the removal of missing parts on the capillary fixture.

[0036] Step 7: When a capillary fixture arrives at the cap loading station, the cap loading mechanism outputs the sample loading caps one by one and transfers them to the top of the PCR capillary for pressing in.

[0037] Step 8: When a capillary fixture arrives at the cover loading station, the unloading mechanism places the assembled PCR capillary on the tray for output.

[0038] The beneficial effects of this invention are:

[0039] 1. This invention enables the automatic assembly, leak testing, and capping of PCR capillary tubes by sequentially passing the capillary fixture through the tube body loading station, sample connector loading station, hot pressing docking station, leak detection station, leak component rejection station, material replenishment station, cap loading station, and unloading station, thus realizing fully automated assembly of PCR capillary tubes with quality inspection procedures.

[0040] 2. The capillary fixture in this invention is provided with independent thermocompression mounting positions and capping mounting positions; the two sets of mounting positions can respectively expose the connection between the capillary body and the sample loading connector, and provide support for the sample loading connector of the PCR capillary obtained by thermocompression. In conjunction with the PCR capillary transfer operation of the feeding mechanism, the thermocompression docking and capping operation of the PCR capillary are completed on the same fixture.

[0041] 3. The leak detection mechanism in this invention converts the invisible gas leak into a visible bubble by inserting the PCR capillary into the leak detection hole and pressurizing it, and by connecting the bottom of the leak detection hole to a water tank. Combined with existing image recognition algorithms, it realizes fully automated leak detection of the PCR capillary. Attached Figure Description

[0042] Figure 1 This is a schematic diagram of the cross-sectional structure of a PCR capillary.

[0043] Figure 2 This is a schematic diagram of the overall structure of the present invention;

[0044] Figure 3This is a schematic diagram of the workstation switching mechanism in this invention;

[0045] Figure 4a This is the first structural schematic diagram of the capillary fixture in this invention;

[0046] Figure 4b This is the second structural schematic diagram of the capillary fixture in this invention;

[0047] Figure 5 This is a cross-sectional view of the capillary fixture in this invention;

[0048] Figure 6 This is a schematic diagram of the tube feeding mechanism in this invention;

[0049] Figure 7 This is a schematic diagram of the rotary orientation assembly in this invention. Figure 6 (Enlarged view of part A in the middle)

[0050] Figure 8 This is a schematic diagram showing the relative positions of the tube body direct vibration machine and the rotary orientation assembly in this invention;

[0051] Figure 9 This is a schematic diagram of the feeding mechanism in this invention;

[0052] Figure 10 This is a schematic diagram of the hot-pressing docking mechanism in this invention;

[0053] Figure 11 This is a schematic diagram of the leak detection mechanism in this invention;

[0054] Figure 12 This is a schematic diagram showing the relative positions of the leak detection transfer component and the leak detection mounting block in this invention;

[0055] Figure 13 This is a schematic diagram of the missing part rejection mechanism in this invention;

[0056] Figure 14 This is a schematic diagram of the feeding mechanism in this invention;

[0057] Figure 15 This is a schematic diagram of the cover feeding mechanism in this invention;

[0058] Figure 16 This is a schematic diagram of the feeding mechanism in this invention;

[0059] Figure 17 This is a schematic diagram showing the relative positions of the empty disk supply component and the full disk output component in this invention.

[0060] Reference numerals: 100, Sample filling cap; 200, Capillary body; 300, Sample filling connector; 1, Station switching mechanism; 2, Tube body feeding mechanism; 3, Sample filling connector feeding mechanism; 4, Hot pressing docking mechanism; 5, Leak detection mechanism; 6, Leaking component rejection mechanism; 7, Material replenishment mechanism; 8, Cap filling mechanism; 9, Unloading mechanism; 1-1, Circular track; 1-2, Capillary fixture; 1-3, Fixture positioning assembly; 1-4, Circulation drive mechanism; 1-5, Sliding plate; 1-2-1, Connecting plate; 1-2-2, Capillary mounting block; 1 -2-3, Guide rod; 1-2-4, Lifting support plate; 1-2-5, Spring; 1-2-6, Limiting block; 1-2-7, Hot press mounting position; 1-2-8, Cover mounting position; 1-2-9, Hot air channel slot; 2-1, Tube feeding base; 2-2, Tube arrangement output assembly; 2-2-1, Tube vibratory plate; 2-2-2, Tube direct vibrator; 2-3, Rotary adjustment assembly; 2-4, Tube transfer assembly; 2-3-1, Rotary plate; 2-3-2, Rotary drive mechanism; 2-3-3, Capillary insertion hole; 2-3- 4. Capillary detection port; 3-1. Connector feeding base; 3-2. Connector arrangement output assembly; 3-3. Connector transfer assembly; 4-1. Hot press bracket; 4-2. Upper pressure assembly; 4-3. Lower pressure assembly; 4-4. Heating assembly; 5-1. Leak detection bracket; 5-2. Sealing block; 5-3. Leak detection air tube; 5-4. Leak detection transfer assembly; 5-5. Leak detection mounting block; 5-6. Lifting and pressurizing assembly; 5-7. Water tank; 5-8. Camera; 5-9. Pressurizing connector; 6-1. Rejection bracket; 6-2. Waste box; 6-3. 7-1. Removal and transfer assembly; 7-2. Feeding assembly; 7-3. Feeding and transfer assembly; Cover loading mechanism: 8-1. Cover support; 8-2. Cover arrangement and output assembly; 8-3. Cover transfer and installation assembly; 9-1. Unloading support; 9-2. Receiving assembly; 9-3. First unloading and transfer assembly; 9-4. Second unloading and transfer assembly; 9-5. Tray in / out assembly; 9-5-1. Tray storage seat; 9-5-2. Tray lateral movement assembly; 9-5-3. Empty tray supply assembly; 9-5-4. Full tray output assembly. Detailed Implementation

[0061] The present invention will be further described below with reference to the accompanying drawings.

[0062] like Figure 2 As shown, a fully automated PCR capillary assembly device includes a frame, and a station switching mechanism 1, a tube feeding mechanism 2, a sample connector feeding mechanism 3, a thermocompression docking mechanism 4, a leak detection mechanism 5, a leak component rejection mechanism 6, a replenishment mechanism 7, a cap feeding mechanism 8, and a discharge mechanism 9, all mounted on the frame.

[0063] like Figure 3As shown, the workstation switching mechanism 1 includes a circular track 1-1, sliding plates 1-5, fixture positioning components 1-3, and a cyclic drive mechanism 1-4. The horizontally arranged circular track 1-1 is fixed to the frame; the circular track 1-1 includes two alternating adjacent straight segments and two circular arc segments; multiple sliding plates 1-5 are sequentially and alternately slidably connected to the circular track 1-1 and can cyclically move along the circular track 1-1. Each sliding plate 1-5 is equipped with a capillary fixture 1-2.

[0064] like Figure 4a , 4b As shown, the capillary fixture 1-2 includes a connecting plate 1-2-1 and a fixture body. The connecting plate 1-2-1 is fixed to the sliding plate. The fixture body includes a capillary mounting block 1-2-2, a guide rod 1-2-3, a lifting support plate 1-2-4, a spring 1-2-5, and a limiting block 1-2-6. The side of the capillary mounting block 1-2-2 is fixed to the edge of the connecting plate 1-2-1. The bottom of the capillary mounting block 1-2-2 is suspended.

[0065] Two vertically arranged guide rods 1-2-3 are fixed to the capillary mounting block 1-2-2. The bottom ends of the guide rods 1-2-3 extend beyond the bottom surface of the capillary mounting block 1-2-2. A lifting support plate 1-2-4 is slidably connected to the guide rods 1-2-3 and is located directly below the capillary mounting block 1-2-2. A limit part is provided at the bottom end of the guide rods 1-2-3 to constrain the lower limit position of the lifting support plate 1-2-4. A limit block 1-2-6 is fixed to the top surface of the lifting support plate 1-2-4 to constrain the upper limit position of the lifting support plate 1-2-4. A spring 1-2-5 is sleeved on the guide rods 1-2-3. The two ends of the spring 1-2-5 abut against the bottom surface of the capillary mounting block 1-2-2 and the top surface of the lifting support plate 1-2-4, respectively.

[0066] like Figure 5 As shown, the capillary mounting block 1-2-2 has two sets of mounting positions: a hot-press mounting position 1-2-7 and a cap mounting position 1-2-8. Both the hot-press mounting position 1-2-7 and the cap mounting position 1-2-8 include a connector positioning hole section located above and a tube body positioning hole section located below. The tube body positioning hole section and the connector positioning hole section can provide radial positioning for the capillary body 200 and the sample dispensing connector 300, respectively. A hot air channel groove 1-2-9 is provided on the side of the capillary mounting block 1-2-2. The hot air channel groove 1-2-9 cuts off the hot-press mounting position 1-2-7, and the connection between the sequentially inserted capillary body and the sample dispensing connector is located in the hot air channel groove, so that during processing, hot air can be directly blown at the connection between the sample dispensing connector 300 and the capillary body 200.

[0067] There are n hot-press mounting positions 1-2-7 and n covered mounting positions 1-2-8; each hot-press mounting position 1-2-7 is located directly above the lifting support plate 1-2-4. In this embodiment, n = 2; in other embodiments, n can also be other numbers.

[0068] In the hot-press installation position 1-2-7, the connector positioning hole section is only above the hot air channel groove 1-2-9; the tube positioning hole section is only below the hot air channel groove 1-2-9; the lifting support plate 1-2-4 provides axial restraint for the bottom end of the capillary body 200. The sample feeding connector 300 is supported by the capillary body 200.

[0069] In the capping installation position 1-2-8, the tube positioning hole section extends above the hot air channel groove 1-2-9, so that a stepped surface is formed between the connector positioning hole section and the tube positioning hole section; the stepped surface provides a limit for the sample dispensing connector 300; while the capillary body 200 has been fixed to the bottom of the sample dispensing connector 300 during the hot pressing stage.

[0070] The station switching mechanism 1 has eight stations, namely: pipe body loading station, sample connector loading station, hot pressing and joining station, leak detection station, leak component rejection station, replenishment station, cover loading station, and unloading station. Pipe body loading mechanism 2, sample connector loading mechanism 3, hot pressing and joining mechanism 4, leak detection mechanism 5, leak component rejection mechanism 6, replenishment mechanism 7, cover loading mechanism 8, and unloading mechanism 9 correspond to the eight stations mentioned above.

[0071] The cyclic drive mechanism 1-4 drives each capillary fixture 1-2 sequentially through eight stations. It includes pulleys, a drive belt, and a station switching motor. Two pulleys, spaced apart, are rotatably connected to the frame and connected by the drive belt. The axes of the two pulleys are collinear with the centers of the two arc segments of the circular track 1-1. The connecting plates 1-2-1 on each capillary fixture 1-2 are fixed to the drive belt. One of the pulleys is driven to rotate by the station switching motor.

[0072] Each workstation corresponds to a fixture positioning assembly 1-3. The fixture positioning assembly 1-3 is used to position the capillary fixture 1-2, ensuring that the capillary fixture 1-2 stops precisely at the corresponding workstation. The fixture positioning assembly 1-3 includes a positioning fork and a telescopic drive assembly. Positioning rollers are rotatably connected to the outer edges of each sliding plate 1-5. The positioning fork is slidably connected to the frame in the vertical direction, and the telescopic drive assembly drives its sliding motion. A positioning groove is formed at the top of the positioning fork. The width of the positioning groove is equal to the diameter of the positioning roller. A chamfer is provided at the top opening of the positioning groove. When a capillary fixture 1-2 is in a workstation, the positioning roller on the capillary fixture 1-2 is aligned with the positioning fork on the fixture positioning assembly 1-3 corresponding to that workstation. By extending the positioning fork (in the state where the motor in the cyclic drive mechanism 1-4 is released from self-locking), the positioning roller is engaged in the positioning groove, thereby adjusting the position of the capillary fixture 1-2 and achieving precise positioning of the fixture.

[0073] like Figure 6 As shown, the tube feeding mechanism 2 includes a tube feeding base 2-1, and a tube arrangement output assembly 2-2, a rotation and orientation assembly 2-3, and a tube transfer assembly 2-4 mounted on the tube feeding base 2-1. The tube feeding base 2-1 is fixed to the frame. The tube arrangement output assembly 2-2 includes a tube vibratory plate 2-2-1 and a tube straight vibrator 2-2-2. The output end of the tube vibratory plate 2-2-1 is connected to the input end of the tube straight vibrator 2-2-2 via a connecting rail. The tube vibratory plate 2-2-1 and the tube straight vibrator 2-2-2 can arrange and output the stacked capillary bodies 200 one by one. The output end of the tube straight vibrator 2-2-2 is provided with a material-pushing mechanism, which can push away the capillary bodies with open ends facing forward, so that all the capillary bodies 200 output by the tube straight vibrator 2-2-2 have closed sections facing forward. The material-pushing mechanism is prior art and will not be described in detail here.

[0074] like Figure 7 and 8 As shown, the rotary orientation assembly 2-3 includes a rotary disk 2-3-1, a rotary drive mechanism 2-3-2, and a tube detection sensor. The rotary disk 2-3-1 is rotatably connected to the tube loading base 2-1 via a rotating shaft. The rotary drive mechanism 2-3-2 drives the rotary disk 2-3-1 to rotate via a motor and a synchronous belt drive structure. The rotary disk 2-3-1 is aligned with the output end of the tube vibrating machine 2-2-2. The axis of the rotary disk 2-3-1 is horizontally set and at the same height as the output port of the corresponding tube vibrating machine 2-2-2.

[0075] Four capillary insertion holes 2-3-3 are evenly distributed circumferentially along their own axis on the outer circumferential surface of the rotating disk 2-3-1. The axis of the capillary insertion holes 2-3-3 is arranged radially along the rotating disk 2-3-1. The capillary insertion holes 2-3-3 facing the tube body vibrator 2-2-2 can receive the capillary body 200 with its closed end facing forward, output from the tube body vibrator 2-2-2. By rotating the rotating disk 2-3-1 by 90°, the horizontal capillary can be adjusted to a vertical state with its opening facing upward.

[0076] Four capillary detection holes 2-3-4 are provided on the end face of the rotating disk 2-3-1. Each of the four capillary detection holes 2-3-4 corresponds to one of the four capillary insertion holes 2-3-3. The capillary detection holes 2-3-4 are through holes and communicate with their corresponding capillary insertion holes 2-3-3. The tube detection sensor is an infrared sensor, fixed on the tube loading base 2-1 and facing the corresponding rotating disk 2-3-1. When a capillary insertion hole 2-3-3 is aligned with the output port of the tube vibrator 2-2-2, the corresponding capillary detection hole 2-3-4 is aligned with the detection part of the tube detection sensor, enabling the tube detection sensor to detect whether a capillary body 200 is inserted into the capillary insertion hole 2-3-3, thus facilitating the rotation of the rotating disk 2-3-1 to adjust the posture of the capillary body 200.

[0077] The tube transfer assembly 2-4 is used to grip the capillary body 200 with its vertically upward-facing opening on the rotary orienting assembly 2-3 and clamp it onto the hot-press mounting position 1-2-7 of the capillary fixture 1-2 at the tube loading station. The tube transfer assembly 2-4 includes a two-axis slide and tube gripping claws. The end mounting plate of the two-axis slide is capable of two degrees of freedom of movement in both vertical and horizontal directions. The tube gripping claws are fixed to the end mounting plate of the two-axis slide via gripper brackets and are used to grip the capillary body 200. The number and relative position of the tube gripping claws correspond to the rotary disk 2-3-1. In this embodiment, the two-axis slide is driven by two cylinders; the tube gripping claws are pneumatic grippers. In other embodiments, the two-axis slide can also be driven by a motor; the tube gripping claws can also be motor-driven robotic grippers.

[0078] like Figure 9As shown, the sample feeding mechanism 3 includes a sample feeding base 3-1, and a sample arrangement output assembly 3-2 and a sample transfer assembly 3-3 mounted on the sample feeding base 3-1. The sample feeding base 3-1 is fixed to the frame. The sample arrangement output assembly 3-2 includes a sample vibrating plate and a sample direct vibrator. The output end of the sample vibrating plate is connected to the input end of the sample direct vibrator via a connecting rail. The sample vibrating plate and the sample direct vibrator can arrange and output the stacked sample feeding connectors 300 one by one. The output sample feeding connectors 300 are positioned with the insertion part connected to the capillary body 200 facing downwards.

[0079] The connector transfer assembly 3-3 is used to pick up the sample connector 300 output from the connector arrangement output assembly 3-2 and clamp it onto the hot-press mounting position 1-2-7 of the capillary fixture 1-2 at the sample connector loading station. The connector transfer assembly 3-3 includes a two-axis connector slide and a connector suction cup. The end mounting plate of the two-axis connector slide is capable of two degrees of freedom of movement in both vertical and horizontal directions. The connector suction cup is mounted on the end mounting plate of the two-axis connector slide and is used to pick up the sample connector 300. The number and relative position of the connector suction cups correspond to the connector linear vibrator. In this embodiment, the two-axis connector slide is driven by two cylinders. In some other embodiments, the two-axis connector slide may also be driven by a motor.

[0080] like Figure 10 As shown, the hot-press docking mechanism 4 includes a hot-press support 4-1, an upper pressing assembly 4-2, a lower pressing assembly 4-3, and a heating assembly 4-4. The hot-press support 4-1 is fixed to the machine frame. The upper pressing assembly 4-2 and the lower pressing assembly 4-3 are respectively positioned directly above and below the hot-press docking station. The upper pressing assembly 4-2 includes an upper pressing plate and an upper pressing cylinder. The lower pressing assembly 4-3 includes a lower pressing plate and a lower pressing cylinder. The upper pressing cylinder and the lower pressing cylinder are fixed to the hot-press support 4-1. The push rod of the upper pressing cylinder faces downward and is fixed to the upper pressing plate. The push rod of the lower pressing cylinder faces upward and is fixed to the lower pressing plate. The heating assembly 4-4 includes a hot air gun. The hot air guns are horizontally positioned with their outlets facing the hot air channel groove 1-2-9 of the capillary mounting block 1-2-2 of the capillary fixture 1-2 at the hot-pressing docking station. Furthermore, each hot air gun is aligned with each hot-pressing mounting position 1-2-7 of the capillary mounting block 1-2-2 at the hot-pressing docking station. During the hot-pressing process, the hot air blown from each hot air gun heats the connection between the capillary body 200 and the sample dispensing connector 300. The upper pressure plate presses downwards against the sample dispensing connector 300 on the capillary fixture 1-2, while the lower pressure plate presses upwards against the bottom lifting support plate 1-2-4 of the capillary fixture 1-2. The lifting support plate 1-2-4 pushes the capillary body 200 upwards, causing the capillary body 200 to be inserted into the sample dispensing connector 300.

[0081] like Figure 11 and 12As shown, the leak detection mechanism 5 includes a leak detection bracket 5-1, a sealing block 5-2, a leak detection air pipe 5-3, a leak detection transfer assembly 5-4, a leak detection mounting block 5-5, a lifting and pressurizing assembly 5-6, a water tank 5-7, and a camera 5-8. The leak detection bracket 5-1 is fixed to the frame. The leak detection mounting block 5-5, the water tank 5-7, and the camera 5-8 are all fixed to the leak detection bracket 5-1. The top of the water tank 5-7 is open; the camera 5-8 is located directly above the water tank 5-7 and faces downwards. The camera 5-8 is used to capture images of the water tank 5-7 and transmit them to the host computer to determine whether air bubbles appear in the water tank 5-7.

[0082] A sealing block 5-2 is embedded and fixed in the leak detection mounting block 5-5; a leak detection hole is provided on the sealing block 5-2. The leak detection hole is a through hole, and its diameter is larger than the diameter of the capillary tube but smaller than the bottom diameter of the sampling tip; this allows the capillary tube, formed by heat pressing, to be inserted into the leak detection hole. Each leak detection hole corresponds to a leak detection air tube 5-3. One end of the leak detection air tube 5-3 is connected to the bottom end of the leak detection hole; the other end of the leak detection air tube 5-3 extends into the water tank 5-7.

[0083] The lifting and pressurizing assembly 5-6 includes a lifting and pressurizing cylinder, a pressurizing mounting plate, and a pressurizing connector 5-9. The lifting and pressurizing cylinder is fixed to the leak detection bracket 5-1. The push rod of the lifting and pressurizing cylinder faces directly downwards and is fixed to the pressurizing mounting plate; the pressurizing connector 5-9 is fixed to the pressurizing mounting plate. The pressurizing connector 5-9 is located directly above the leak detection hole. Each pressurizing connector 5-9 is connected to a gas source via a gas supply pipe.

[0084] The leak detection transfer assembly 5-4 is used to transfer the capillary tube, which has been thermo-pressed and is located on the capillary fixture 1-2 at the leak detection station, to the leak detection mounting block 5-5, and to return the capillary tube after leak detection to the thermo-pressed mounting position 1-2-7 of the capillary fixture 1-2. The leak detection transfer assembly 5-4 includes a two-axis leak detection slide and a leak detection suction cup. The end mounting plate of the two-axis leak detection slide is capable of two degrees of freedom of movement in both vertical and horizontal directions. The leak detection suction cup is mounted on the end mounting plate of the two-axis leak detection slide and is used to aspirate the PCR capillary tube. In this embodiment, the two-axis leak detection slide is driven by two cylinders. In some other embodiments, the two-axis leak detection slide may also be driven by a motor.

[0085] During leak testing, water tank 5-7 is filled with water, and the end of leak detection tube 5-3 is submerged below the liquid surface. Leak transfer assembly 5-4 transfers the PCR capillary to the leak detection port. The pressurizing connector 5-9 of the lifting and pressurizing assembly 5-6 connects to the sample loading connector 300 on the PCR capillary and pressurizes the PCR capillary. If there is a sealing defect at the connection between the capillary body 200 and the sample loading connector 300, the gas input to the PCR capillary will leak out and be transmitted through the leak detection port and leak detection tube 5-3 to the water tank 5-7, generating bubbles. Therefore, whether the PCR capillary is leaking can be determined by whether bubbles are generated in the water tank 5-7. The host computer uses an image recognition algorithm to automatically identify whether bubbles are present in the image captured by camera 5-8.

[0086] like Figure 13 As shown, the defective component rejection mechanism 6 includes a rejection bracket 6-1, a waste container 6-2, and a rejection transfer assembly 6-3. The rejection bracket 6-1 is fixed to the frame; the waste container 6-2 is fixed to the rejection bracket 6-1 and has an open top. The rejection transfer assembly 6-3 is used to transfer PCR capillaries identified as leaking from the capillary fixture 1-2 at the defective component rejection station to the waste container 6-2; the rejection transfer assembly 6-3 includes a two-axis rejection slide and rejection suction cups. The end mounting plate of the two-axis rejection slide can move with two degrees of freedom in both vertical and horizontal directions. n rejection suction cups are mounted side by side on the end mounting plate of the two-axis rejection slide for aspirating PCR capillaries. The n rejection suction cups are connected to a negative pressure source through independent solenoid valves, allowing independent control of whether to aspirate. The two-axis rejection slide can drive the rejection suction cup waste box 6-2 to reciprocate between the area above the defective part rejection station, transferring the PCR capillary tubes on the capillary fixture 1-2 at the defective part rejection station to the suction cup waste box 6-2. In this embodiment, the two-axis rejection slide is driven by two cylinders. In some other embodiments, the two-axis rejection slide may also be driven by a motor.

[0087] like Figure 14 As shown, the feeding mechanism 7 includes a feeding support 7-1, a feeding assembly 7-2, and a feeding transfer assembly 7-3. The feeding support 7-1 is fixed to the frame; the feeding assembly 7-2 includes a feeding transverse assembly and a feeding seat. The feeding transverse assembly adopts a single-degree-of-freedom linear module; the feeding seat is installed on the feeding transverse assembly. The feeding seat is used to place a tray containing a matrix of PCR capillary tubes (without caps). The PCR capillary tubes on the tray are manually replaced after being removed; since the probability of PCR capillary tube leakage requiring refilling is low, and multiple PCR capillary tubes can be stored on one tray, the frequency of manual tray replacement is low, and the impact on automated production is minimal.

[0088] The feed transfer assembly 7-3 is used to transfer PCR capillaries from the feed holder to the capillary fixture 1-2 (at the feed station), filling vacant thermocompression mounting positions 1-2-7, and transferring PCR capillaries from thermocompression mounting positions 1-2-7 of the capillary fixture 1-2 to capping mounting positions 1-2-8. The feed transfer assembly 7-3 includes a two-axis feed slide and feed suction cups. The end mounting plate of the two-axis feed slide is capable of two degrees of freedom of movement in both vertical and horizontal directions. n feed suction cups are mounted side-by-side on the end mounting plate of the two-axis feed slide for aspirating PCR capillaries. Each feed suction cup is connected to a negative pressure source via an independent solenoid valve, allowing independent control of suction. The two-axis feed slide can drive the feed assembly 7-2 to reciprocate between the feed station and the top of the feed station, transferring PCR capillaries placed on the feed holder to the capillary fixture 1-2 at the feed station. In this embodiment, the horizontal movement of the two-axis feeding slide is driven by a motor and can stop at any position, while the vertical movement is driven by a cylinder. In some other embodiments, the vertical movement of the two-axis feeding slide can also be driven by a motor. The horizontal movement direction of the two-axis feeding slide is perpendicular to the movement direction of the feeding transverse movement assembly.

[0089] like Figure 1 and 15 As shown, the cover feeding mechanism 8 includes a cover support 8-1, and a cover arrangement output assembly 8-2 and a cover transfer mounting assembly 8-3 mounted on the cover support 8-1. The cover support 8-1 is fixed to the frame. The cover arrangement output assembly 8-2 includes a cover vibratory feeder and a cover direct vibrator. The output end of the cover vibratory feeder is connected to the input end of the cover direct vibrator via a connecting rail. The cover vibratory feeder and the cover direct vibrator are capable of arranging and outputting stacked sample covers 100 one by one. The number of cover direct vibrators is n; two cover direct vibrators are arranged side by side.

[0090] The cap transfer and mounting assembly 8-3 is used to grip the cap output from the cap vibratory extruder and clamp it onto the cap mounting position 1-2-8 of the capillary fixture 1-2 at the cap loading station, pressing it into the top of the PCR capillary. The cap transfer and mounting assembly 8-3 includes a two-axis slide and a cap gripper. The end mounting plate of the two-axis slide is capable of two degrees of freedom of movement in both vertical and horizontal directions. The cap gripper is fixed to the end mounting plate of the two-axis slide via a gripper bracket and is used to grip the sample loading cap 100. In this embodiment, the two-axis slide is driven by two cylinders; the cap gripper is a pneumatic gripper. In other embodiments, the two-axis slide may also be driven by a motor; the cap gripper may also be a motor-driven robotic gripper.

[0091] like Figure 16 and 17As shown, the unloading mechanism 9 includes an unloading bracket 9-1, and a receiving assembly 9-2, a first unloading transfer assembly 9-3, a second unloading transfer assembly 9-4, and a tray in / out assembly 9-5 mounted on the unloading bracket 9-1. The receiving assembly 9-2 includes a receiving seat and a receiving transverse movement assembly. The receiving transverse movement assembly adopts a single-degree-of-freedom linear module; the receiving seat is mounted on the receiving transverse movement assembly. The receiving seat is used to hold empty trays.

[0092] The first feeding and transfer component 9-3 is used to pick up PCR capillary tubes (with caps) from the capillary fixture 1-2 at the feeding station and transfer them to the receiving component 9-2. The first feeding and transfer component 9-3 includes a first feeding two-axis slide and a feeding suction cup. The end mounting plate of the first feeding two-axis slide is capable of two degrees of freedom of movement in both vertical and horizontal directions. The feeding suction cup is mounted on the end mounting plate of the first feeding two-axis slide and is used to pick up PCR capillary tubes. In this embodiment, the horizontal movement of the first feeding two-axis slide is driven by a motor and can stop at any position, while the vertical movement is driven by a cylinder. In some other embodiments, the vertical movement of the first feeding two-axis slide can also be driven by a motor. The horizontal movement direction of the first feeding two-axis slide is perpendicular to the movement direction of the receiving transverse component. The first feeding and transfer component 9-3 cooperates with the receiving transverse component to transfer the PCR capillary tubes from the capillary fixture 1-2 to the receiving seat.

[0093] The tray loading / unloading assembly 9-5 is used to provide empty trays to the receiving assembly 9-2 and to receive trays filled with PCR capillaries. The tray loading / unloading assembly 9-5 includes a tray storage seat 9-5-1, a tray traversing assembly 9-5-2, an empty tray supply assembly 9-5-3, and a full tray output assembly 9-5-4. The tray storage seat 9-5-1 is slidably connected to the unloading bracket 9-1 and is driven by the tray traversing assembly 9-5-2.

[0094] An empty tray supply assembly 9-5-3 and a full tray output assembly are mounted side-by-side on a tray storage base 9-5-1. The empty tray supply assembly 9-5-3 includes a tray fixture and an empty tray ejection assembly. The tray fixture is fixed to the tray storage base 9-5-1 and can stack multiple empty trays. The empty tray ejection assembly is used to eject the bottom empty tray from the tray fixture.

[0095] The full-tray output assembly includes a full-tray output plate and a tray transfer assembly. The full-tray output plate is slidably connected to the tray storage base 9-5-1 and is moved by the tray transfer assembly, which uses a cylinder. The trays filled with PCR capillaries, placed on the full-tray output plate, are either manually removed and boxed or packaged using a rear-mounted automatic tray boxing mechanism.

[0096] The second unloading transfer assembly 9-4 cooperates with the tray traversing assembly 9-5-2 to transfer empty trays from the empty tray supply assembly 9-5-3 to the receiving seat of the receiving assembly 9-2, and to transfer full trays from the receiving seat of the receiving assembly 9-2 to the full tray output plate of the full tray output assembly. The second unloading transfer assembly 9-4 includes a second unloading two-axis slide and unloading clamping claws. The end mounting plate of the second unloading two-axis slide is capable of two degrees of freedom of movement in both vertical and horizontal directions. The unloading clamping claws are mounted on the end mounting plate of the second unloading two-axis slide and are used to clamp the tray. In this embodiment, both the horizontal and vertical movements of the second unloading two-axis slide are driven by cylinders. In other embodiments, the horizontal and vertical movements of the second unloading two-axis slide may also be driven by motors. The horizontal movement direction of the second unloading two-axis slide is perpendicular to the movement direction of the tray traversing assembly 9-5-2.

[0097] The number of hot-press mounting positions 1-2-7, cap mounting positions 1-2-8, the channel of the tube body direct vibrator 2-2-2, the rotating disk 2-3-1, the tube body clamping claw, the channel of the connector direct vibrator, the connector suction cup, the hot air gun, the leak test hole, the pressure connector 5-9, the leak test suction cup, the rejection suction cup, the feeding suction cup, the cap body clamping claw, and the first feeding suction cup are all equal, n, and their positions correspond, which can realize the synchronous assembly of n PCR capillaries on the same fixture.

[0098] The assembly method of this fully automated PCR capillary assembly device is as follows:

[0099] Step 1: The station switching mechanism 1 drives each capillary fixture 1-2 to perform cyclical movement, passing through the tube body loading station, sample connector loading station, hot pressing docking station, leak detection station, leaking part rejection station, replenishment station, cover loading station and unloading station in sequence.

[0100] Step 2: When a capillary fixture 1-2 arrives at the tube loading station, the tube loading mechanism 2 outputs the capillary body 200 one by one and places it vertically on the hot-press mounting position 1-2-7 on the capillary fixture 1-2.

[0101] Step 3: When a capillary fixture 1-2 arrives at the sample feeding station, the sample feeding mechanism 3 outputs the sample feeding connectors 300 one by one and places them vertically on the hot-press mounting positions 1-2-7 on the capillary fixture 1-2. At this time, the top opening of the capillary body 200 is aligned with the bottom end of the sample feeding connector 300.

[0102] Step 4: When a capillary fixture 1-2 arrives at the thermocompression docking station, the hot air gun in the thermocompression docking mechanism 4 blows out hot air, and the upper pressure component 4-2 and the lower pressure component 4-3 are pushed out simultaneously, applying pressure to the sample dispensing connector 300 and the capillary body 200, so that the bottom opening of the sample dispensing connector 300 is inserted and fixed together with the top opening of the capillary body 200, forming an uncapped PCR capillary.

[0103] Step 4: When a capillary fixture 1-2 reaches the leak detection station, the leak detection transfer component 5-4 in the leak detection mechanism 5 transfers the PCR capillary to the leak detection hole in the sealing block 5-2. The lifting and pressurizing component 5-6 pressurizes the inner cavity of the PCR capillary; in the event of a PCR capillary leak, bubbles will appear in the water tank 5-7. The camera 5-8 captures an image of the liquid level in the water tank 5-7; the host computer uses this image to determine whether the PCR capillary is leaking.

[0104] Step 5: When a capillary fixture 1-2 arrives at the leak removal station, the leak removal mechanism 6 removes the leaking PCR capillary into the waste bin based on the detection results of the PCR capillary at the leak detection station.

[0105] Step 6: When a capillary fixture 1-2 arrives at the feeding station, the feeding mechanism 7 places a pre-prepared uncapped PCR capillary into the thermo-press mounting position 1-2-7 on the empty capillary fixture 1-2, and transfers the PCR capillary in the thermo-press mounting position 1-2-7 to the capping mounting position 1-2-8.

[0106] Step 7: When a capillary fixture 1-2 arrives at the cap loading station, the cap loading mechanism 8 outputs the sample loading cap 100 one by one and transfers it to the top of the PCR capillary for pressing in, thus completing the PCR capillary assembly process.

[0107] Step 8: When a capillary fixture 1-2 arrives at the cap loading station, the unloading mechanism 9 places the assembled PCR capillary onto the tray of the receiving seat. When the tray on the receiving seat is full, the unloading mechanism 9 outputs the full tray and places an empty tray on the receiving seat.

Claims

1. A PCR capillary full-automatic assembly equipment, comprising a rack, a station switching mechanism (1); characterized in that: Also include the corresponding different station position of the station switching mechanism (1) respectively position cap body feeding mechanism (8) and unloading mechanism (9); The station switching mechanism (1) is provided with a plurality of capillary fixtures (1-2);Each capillary fixture (1-2) can be circulated and move through each station in turn;The capillary fixture (1-2) is used for providing positioning and support for the capillary body and the sample adding connector; The cap body feeding mechanism (8) is used for installing the cap on the sample adding connector of the PCR capillary; The unloading mechanism (9) is used for moving the PCR capillary with the cap to the capillary fixture (1-2). The capillary fixture (1-2) includes a capillary mounting block (1-2-2), a lifting support plate (1-2-4) and a limiting block (1-2-6);The lifting support plate (1-2-4) is slidably connected below the capillary mounting block (1-2-2);The capillary mounting block (1-2-2) is provided with a hot pressing mounting position (1-2-7) and a capping mounting position (1-2-8);Each hot pressing mounting position (1-2-7) is located directly above the lifting support plate (1-2-4);The hot pressing mounting position (1-2-7) and the capping mounting position (1-2-8) each include a connector positioning hole section located above and a capillary body positioning hole section located below;The side of the capillary mounting block (1-2-2) is provided with a hot air channel groove (1-2-9) for cutting off the hot pressing mounting position (1-2-7);During the hot pressing connection process, the connecting part of the sample adding connector and the capillary body is exposed in the hot air channel groove (1-2-9);The capping mounting position (1-2-8) is provided with a stepped surface for supporting the sample adding connector. ​ ​ ​ 2. The PCR capillary full-automatic assembling equipment according to claim 1, characterized in that: ​ 3. The PCR capillary full-automatic assembling equipment according to claim 1, characterized in that: The station switching mechanism (1) comprises a ring track (1-1), a capillary tube jig (1-2), a circulating driving mechanism (1-4) and a sliding plate (1-5); the ring track (1-1) is fixed on a rack; a plurality of sliding plates (1-5) are arranged in sequence on the ring track (1-1) and can move in a circulating manner along the ring track (1-1) under the driving of the circulating driving mechanism (1-4); the capillary tube jig (1-2) is installed on the sliding plate (1-5).

4. The PCR capillary full-automatic assembling equipment according to claim 1, characterized in that: Each station corresponds to a jig positioning assembly (1-3); the jig positioning assembly (1-3) is used for positioning the capillary tube jig (1-2); the jig positioning assembly (1-3) comprises a positioning fork and a telescopic driving assembly; each capillary tube jig (1-2) is provided with a positioning roller correspondingly; the positioning fork is slidably connected to the rack in the vertical direction and is slid by the telescopic driving assembly driven by a drive; a positioning recess is formed at the top end of the positioning fork; a chamfer is arranged at the top opening of the positioning recess; when one capillary tube jig (1-2) is in one station, the positioning roller corresponding to the capillary tube jig (1-2) is aligned with the positioning fork on the jig positioning assembly (1-3) corresponding to the station; the positioning roller is clamped into the positioning recess by the pushing of the positioning fork.

5. The PCR capillary full-automatic assembling equipment according to claim 1, characterized in that: The pipe body loading mechanism (2) comprises a pipe body loading base (2-1), a pipe body arrangement output assembly (2-2), a rotary direction adjusting assembly (2-3) and a pipe body transfer assembly (2-4) installed on the pipe body loading base (2-1); the pipe body loading base (2-1) is fixed with the rack; the pipe body arrangement output assembly (2-2) arranges and outputs the stacked capillary tube body (200) one by one through a pipe body vibrating disc (2-2-1) and a pipe body direct vibration machine (2-2-2); the rotary direction adjusting assembly (2-3) is used for adjusting the capillary tube body (200) output by the pipe body arrangement output assembly (2-2) to an open posture with the vertical upward direction; the pipe body transfer assembly (2-4) is used for clamping the capillary tube body (200) on the rotary direction adjusting assembly (2-3) and clamping it onto the capillary tube jig (1-2).

6. The PCR capillary full-automatic assembling equipment according to claim 2, characterized in that: The air outlets of the hot air guns are directed towards the hot air channel grooves (1-2-9) of the capillary tube jigs (1-2) on the corresponding stations.

7. The PCR capillary full-automatic assembling equipment according to claim 1, characterized in that: The leak detection mechanism (5) comprises a leak detection support (5-1), a sealing block (5-2), a leak detection air pipe (5-3), a leak detection transfer assembly (5-4), a lifting and pressurizing assembly (5-6), a water tank (5-7) and a camera (5-8); the sealing block (5-2) is provided with a leak detection hole; the leak detection hole is in the form of a through hole, and the hole diameter is greater than the diameter of the capillary tube; the PCR capillary tube formed by hot pressing can be inserted into the leak detection hole and supported by the top surface of the sealing block (5-2); the bottom end of the leak detection hole is connected to the water tank (5-7) through the leak detection air pipe (5-3); the lifting and pressurizing assembly (5-6) is used for pressurizing the inner cavity of the PCR capillary tube inserted into the leak detection hole; the leak detection transfer assembly (5-4) is used for transferring the PCR capillary tube on the corresponding capillary tube jig (1-2) to the leak detection hole, and returning the PCR capillary tube after leak detection to the capillary tube jig (1-2); the camera (5-8) is used for shooting the image of the water tank (5-7).

8. The PCR capillary full-automatic assembling equipment according to claim 1, characterized in that: Further comprising a defective piece removing mechanism (6) and a material supplementing mechanism (7); along the moving direction of the capillary tube jig (1-2), the defective piece removing mechanism (6) and the material supplementing mechanism (7) are arranged in sequence between the leak detection mechanism (5) and the cover body feeding mechanism (8); the defective piece removing mechanism (6) is used for removing the PCR capillary tube with leakage detected by the leak detection mechanism (5); the material supplementing mechanism (7) is used for placing a PCR capillary tube without leakage into the position of the capillary tube jig (1-2) vacated due to the removal by the defective piece removing mechanism (6).

9. The PCR capillary full-automatic assembling equipment according to claim 1, characterized in that: The cover body feeding mechanism (8) comprises a cover body support (8-1), and a cover body arrangement and output assembly (8-2) and a cover body transfer and installation assembly (8-3) installed on the cover body support (8-1); the cover body support (8-1) is fixed on the rack; the cover body arrangement and output assembly (8-2) outputs the cover bodies one by one through a cover body vibrating disc and a cover body direct vibration machine; the cover body transfer and installation assembly (8-3) is used for clamping the cover bodies output by the cover body direct vibration machine, and clamping the cover bodies onto the cover installation position (1-2-8) of the capillary tube jig (1-2) at the cover body feeding station, and pressing into the top of the PCR capillary tube.

10. The method of claim 8, wherein the method further comprises: The method comprises the following steps: ​ Step one, each capillary tube jig (1-2) on the station switching mechanism (1) performs cyclic motion, and sequentially passes through the tube body feeding station, the sample joint feeding station, the hot pressing docking station, the leak detection station, the defective piece removing station, the material supplementing station, the cover body feeding station and the discharging station; Step two, when one capillary tube jig (1-2) reaches the tube body feeding station, the tube body feeding mechanism (2) outputs the capillary tube bodies one by one, and vertically places them on the hot pressing installation position (1-2-7) of the capillary tube jig (1-2); Step three, when one capillary tube jig (1-2) reaches the sample joint feeding station, the sample joint feeding mechanism (3) outputs the sample joints one by one, and vertically places them on the hot pressing installation position (1-2-7) of the capillary tube jig (1-2); Step four, when a capillary tool (1-2) reaches the hot pressing butt joint station, the hot pressing butt joint mechanism (4) presses and extrudes the sample adding joint (300), so that the bottom end opening of the sample adding joint (300) is inserted and fixed with the top end opening of the capillary body (200) together to form a PCR capillary; Step four, when a capillary tool (1-2) reaches the leak detection station, the leak detection mechanism (5) pressurizes the inner cavity of the PCR capillary to determine whether the PCR capillary leaks; Step five, when a capillary tool (1-2) reaches the leak removal station, the leak removal mechanism (6) removes the leaked PCR capillary to the waste box according to the detection result of the PCR capillary at the leak detection station; Step six, when a capillary tool (1-2) reaches the material supplement station, the material supplement mechanism (7) puts the pre-prepared uncapped PCR capillary into the vacancy of the capillary tool (1-2) caused by the removal of the leak; Step seven, when a capillary tool (1-2) reaches the cap body loading station, the cap body loading mechanism (8) outputs the sample adding cap body (100) one by one and transfers it to the top end of the PCR capillary for pressing; Step eight, when a capillary tool (1-2) reaches the cap body loading station, the unloading mechanism (9) places the assembled PCR capillary on the tray for output.

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