A fungicidal composition for controlling fungal diseases

Abstract

The present application belongs to the technical field of pesticides, and particularly relates to a fungicidal composition for preventing and treating fungal diseases. The fungicidal composition comprises effective components of rockenbach lactone and benzene propyl thiazole or pyrazole naphthamide. The mass ratio of the rockenbach lactone and the benzene propyl thiazole is 1-9:19-1, and the mass ratio of the rockenbach lactone and the pyrazole naphthamide is 1-30:25-1. When the rockenbach lactone and the benzene propyl thiazole or the pyrazole naphthamide are compounded in the active fungicide, the rockenbach lactone and the benzene propyl thiazole or the pyrazole naphthamide have a synergistic effect, and compared with single components, the active fungicide can improve the prevention and treatment effect on plant diseases, so that the pesticide dosage can be reduced, and environmental pollution and pesticide residues can be reduced. The active fungicide can delay the development of plant disease pathogen resistance, and compared with single components, the active fungicide can reduce the risk of drug resistance.

Description

Technical Field

[0001] This invention belongs to the field of pesticide technology, specifically relating to a fungicidal composition for the prevention and control of fungal diseases. Background Technology

[0002] Wheat stripe rust is caused by the fungus *Strombus styracifolius* (…). Puccinia striiformis f.sp. wheat Stripe rust, a leaf disease caused by infection, is one of the most devastating plant diseases in wheat production. It primarily affects wheat leaves and can occur throughout the entire growing season. Initially, noticeable chlorotic spots appear on diseased leaves, followed by the rapid formation and expansion of raised yellow or bright yellow uredinia. At first, the uredinia are small, appearing as small, yellow, oval protrusions. Later, the leaf epidermis ruptures, exposing a large amount of yellow powder. Wheat stripe rust is characterized by its high explosiveness, rapid spread, and wide distribution, seriously threatening high and stable wheat yields.

[0003] Chemical control is one of the effective ways to control plant diseases. In agricultural production, wheat stripe rust can be controlled by spraying fungicides such as carbendazim, triadimefon, triazole, tebuconazole, hexaconazole, and propiconazole. However, long-term use of single fungicides leads to problems such as increased pathogen resistance, increased pesticide dosage, and aggravated environmental pollution. Combining pesticides with different mechanisms of action can overcome the shortcomings of using single fungicides to some extent and is a common and convenient method for controlling drug-resistant pathogens. Therefore, screening compound formulations for controlling wheat stripe rust is of great significance.

[0004] Bergenin, also known as bergenin, is a coumarin compound belonging to the isocoumarin class of compounds. It is the active ingredient in plants such as *Gnaphalium affine* and *Astilbe* (family Saxifragaceae), as well as *Gnaphalium affine*, *Aralia elata*, and *Gnaphalium affine* (family Myrsinaceae). Currently, bergenin has been proven to possess pharmacological activities including antibacterial, anticancer, anti-HIV, anti-ulcer, antiarrhythmic, antihypertensive, and analgesic effects. Existing technologies have also reported that bergenin can inhibit the growth of plant pathogenic fungi and kill crop mites, showing potential for development into plant-derived fungicides and insecticides.

[0005] There are currently no reports of combining Bergenin with benzylthiazole or pyraclostrobin.

[0006] The information disclosed in this background section is intended only to enhance the understanding of the overall background of the invention and should not be construed as an admission or in any way implying that the information constitutes prior art known to those skilled in the art. Summary of the Invention

[0007] The purpose of this invention is to provide a fungicidal composition for preventing and controlling fungal diseases, which can improve the control effect on plant diseases and reduce environmental pollution and pesticide residues.

[0008] To achieve the above objectives, the present invention provides the following technical solution:

[0009] A bactericidal composition, wherein the active ingredient of the bactericidal composition is a compound of benzyl lactone and phenylpropionylthiazole or pyraclostrobin; wherein,

[0010] The mass ratio of Bergenin to phenylpropenzylthiazole is 1-9:19-1;

[0011] The mass ratio of Bergenin to pyraclostrobin is 1-30:25-1.

[0012] More specifically, the mass ratio of Bergenin to phenylpropenzylthiazole is 3:1.

[0013] More specifically, the mass ratio of Bergenin to pyraclostrobin is 1:7 or 30:1.

[0014] The present invention also provides an active fungicide for the prevention and control of fungal diseases, which is prepared from the fungicidal composition and pesticide-permissible auxiliary ingredients.

[0015] More specifically, the mass of the bactericidal composition accounts for 0.5-80% of the total mass of the active bactericide.

[0016] Compared with the prior art, the present invention has the following beneficial effects:

[0017] (1) When the active fungicide of the present invention is combined with benzylthiazole or pyraclostrobin, it has a synergistic effect. Compared with the single component, it can improve the control effect on plant diseases, thereby reducing the amount of pesticides used, reducing environmental pollution and pesticide residues.

[0018] (2) The active fungicide of the present invention can delay the development of drug resistance in plant pathogens and reduce the risk of drug resistance compared with single components. Detailed Implementation

[0019] The technical solution of this invention patent will be clearly and completely described below. Obviously, the described embodiments are only some, not all, of the embodiments of this invention. Based on the embodiments of this invention, all other embodiments obtained by those skilled in the art without inventive effort are within the scope of protection of this invention.

[0020] examples Indoor bioactivity test

[0021] 1. Test crop

[0022] The two-leaf-one-heart stage "Mingxian 169" wheat seedlings with basically uniform growth, 10 plants per pot.

[0023] 2. Test strains and spore suspension

[0024] Wheat stripe rust ( Puccinia striiformis f.sp. wheat Wheat leaves infected with wheat stripe rust were collected from wheat fields. Leaf segments with relatively dispersed spore clusters were selected, rinsed with water, and then placed in petri dishes with moistened filter paper overnight. After conidia, spores from individual spore clusters were picked up with a sterilized inoculation needle and inoculated onto the leaves of the test crop for propagation. Fresh urediniospores of stripe rust produced within 24 hours on the leaves of the test crop were washed off with a 0.1% Tween-80 aqueous solution, filtered through double-layer gauze, and then diluted with a 0.1% Tween-80 aqueous solution to a concentration of 1×10⁻⁶. 5 A suspension of 1 spore / mL is prepared for later use.

[0025] 4. Test reagents

[0026] 98% Bergenin lactone technical grade (Sigma-Aldrich (Shanghai) Trading Co., Ltd.), 95% Allylfenthiazole technical grade (Yancheng United Weiye Chemical Co., Ltd.), 92% Pyraclostrobin technical grade (Syngenta Nantong Crop Protection Co., Ltd.)

[0027] The test reagent was first dissolved in dimethyl sulfoxide, then diluted with 0.1% Tween-80 aqueous solution to prepare a single-agent stock solution. Multiple formulations were set up, and five mass concentration gradients were set for each single agent and formulation mixture according to the proportional method.

[0028] 5. Test methods (Refer to NY / T 1156.15-2008 Guidelines for Indoor Bioassay of Pesticides - Fungicides Part 15: Pot Test for Control of Wheat Leaf Rust)

[0029] The spore suspension was sprayed onto the test crops and then incubated in the dark at a controlled temperature of 15-20℃ for 12 hours. The crops were then incubated in a greenhouse at 18-22℃ with 12 hours of light per day. 24 hours after inoculation, the test solution was sprayed evenly onto the leaves of the test crops until completely wet. After the solution was allowed to air dry naturally, the plants were moved to a greenhouse at 18-22℃ for further incubation. Four plants were treated with each concentration of the solution, and a blank control containing only a 0.1% Tween-80 aqueous solution was included.

[0030] 6. Tiered investigation of the patient's condition

[0031] When the incidence rate in the blank control group reaches 80% or higher, the incidence rate of each treatment is investigated at different levels, and the disease index and prevention and control effect of each treatment are calculated based on the investigation data.

[0032] Table 1 Disease Grading Table

[0033]

[0034]

[0035]

[0036] 7. Data Analysis

[0037] Data statistical analysis was performed using DPS software. Regression analysis was conducted on the logarithmic values ​​of drug concentrations and the probability of efficacy to calculate the EC50 of each drug. 50 The synergistic coefficient SR of the mixture was calculated using the Wadley method, and the synergistic effect of the drug mixture was evaluated based on the synergistic coefficient SR. The results are shown in Table 2-3.

[0038] Table 2. Indoor bioactivity of Bergenin and allylphenthiazide in *Berberis vulgaris* var. *wheat* stripe rust.

[0039]

[0040] As shown in Table 2, the synergistic effect of bergapten combined with allylbenzylthiazide against wheat stripe rust was greater than 1.5 within a mass ratio of 1-9:19-1, indicating a synergistic effect.

[0041] Table 3. Indoor bioactivity of Bergenin and Pyrazopyr combined with *Berberis vulgaris* against wheat stripe rust.

[0042]

[0043] As shown in Table 3, the synergistic effect of the combination of Bergenin and Pyrazosulfuron against wheat stripe rust was greater than 1.5 within a mass ratio of 1-30:25-1, indicating a synergistic effect.

[0044] In summary, when Bergenin is combined with benzylthiazole or pyraclostrobin, it has a synergistic effect. Compared with single components, it can improve the control effect on plant diseases, thereby reducing pesticide usage, environmental pollution and pesticide residues.

[0045] The foregoing description of specific exemplary embodiments of the invention is for illustrative and explanatory purposes. These descriptions are not intended to limit the invention to the precise forms disclosed, and it will be apparent that many changes and variations can be made in accordance with the foregoing teachings. The exemplary embodiments were chosen and described in order to explain the specific principles of the invention and its practical application, thereby enabling those skilled in the art to implement and utilize various different exemplary embodiments of the invention, as well as various different choices and variations. The scope of the invention is intended to be defined by the claims and their equivalents.

Claims

1. A bactericidal composition, characterized in that, The active ingredient of the bactericidal composition is a compound of Bergenin and phenylpropenzylthiazole; wherein the mass ratio of Bergenin to phenylpropenzylthiazole is 1-9:19-1.

2. The bactericidal composition according to claim 1, characterized in that, The mass ratio of Bergenin to phenylpropenzylthiazole is 3:

1.

3. An active fungicide for controlling fungal diseases, characterized in that, It is prepared from the bactericidal composition according to any one of claims 1-2 and pesticide-permissible auxiliary ingredients.

4. The active fungicide for controlling fungal diseases according to claim 3, characterized in that, The mass of the bactericidal composition accounts for 0.5-80% of the total mass of the active bactericide.

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