Method for detecting soluble dietary fiber in resistant dextrin

The method of directly detecting soluble dietary fiber in resistant dextrin by enzymatic hydrolysis with amylase and high-performance liquid chromatography with external standard chromatography simplifies the operation process, solves the complexity and error problems of existing detection methods, and achieves efficient and accurate detection results.

CN117571875BActive Publication Date: 2026-04-07BY HEALTH CO LTD
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Patent Information

Application Number
CN202311643929.1
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2023-12-01
Publication Date
2026-04-07
Estimated Expiration
2043-12-01

AI Technical Summary

Technical Problem

Existing detection methods for soluble dietary fiber in antagonistic dextrin suffer from problems such as complex procedures, long processing times, multiple sources of error, and unsuitability for large-scale sample testing, especially with large deviations in test results under high purity conditions.

Method used

Amyloglucosidase was used to enzymatically hydrolyze antagonistic dextrin to prepare a test solution. The soluble dietary fiber content was directly calculated using glucose as a standard by high performance liquid chromatography with external standard method, eliminating the alcohol precipitation step and using blank elimination method to eliminate interference, thus simplifying the operation process.

Benefits of technology

It enables rapid and accurate detection of soluble dietary fiber, reduces errors, is suitable for large-scale sample testing, meets quality control standards, and improves testing efficiency and accuracy.

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Abstract

The application belongs to the technical field of food detection, and provides a detection method of soluble dietary fiber in resistant dextrin. The method adopts amylodextrinase to perform enzymatic digestion on the resistant dextrin, adds water to prepare a test solution; glucose is used as a standard, high performance liquid chromatography is used for detection, and the content of the soluble dietary fiber in the resistant dextrin is obtained by an external standard method. The detection method is simpler than the existing standard method, greatly reduces the error sources, can guarantee the accuracy and precision of the detection results, shortens the detection time, saves manpower and economic cost, and can meet the detection needs of inspection supervision departments and enterprises.
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