A device for collecting, transporting and temporarily raising drosophila melanogaster and a method of use

Abstract

The application discloses a kind of taro fruit fly collection, transport and temporary rearing device and method, it is related to insect collection technical field.The storage box is provided with hand strap on the side wall, the top wall and side wall of storage box are provided with vent, the inner wall of storage box is provided with fixed angle code assembly, fixed angle code assembly is provided with fixed clamping plate, fixed clamping plate is provided with first fixed hole, first fixed hole is clamped with containing tube, the bottom of storage box is paved with water-absorbing resin, containing tube is provided with opening at both ends, the top opening of containing tube is covered with gauze, the bottom of containing tube is above water-absorbing resin, and the bottom opening is provided with gauze.The application can quickly and efficiently complete the collection of taro fruit fly together with host, and can extend the normal state of host within a certain period of time, provide survival conditions for taro fruit fly, achieve the purpose of temporary rearing and prevent the host and taro fruit fly from being squeezed during transportation, avoid the death of taro fruit fly during transportation, and effectively improve the collection efficiency.

Description

Technical Field

[0001] This invention relates to the field of insect collection technology, specifically to a device and method for collecting, transporting, and temporarily holding taro fruit flies. Background Technology

[0002] Taro fruit flies belong to the genus *Drosophila* within the subfamily Drosophilinae of the family Drosophila. Almost all fruit flies in this genus depend on plants of the tribe Araceae (coconut tribe) for survival; the two species have a mutually beneficial symbiotic relationship, which is a crucial factor in ecosystem stability. Taro fruit flies frequently congregate in the inflorescences of their host plants in the Araceae family, where they feed, mate, lay eggs, and pollinate, thus forming a mutually beneficial symbiotic relationship with the Araceae plants. When studying this type of mutualistic system or the taro fruit fly, it is often necessary to collect a large number of live taro fruit fly samples and their host plants for various sequencing and analysis. The commonly used collection method is to use a spirochete to suck the fruit flies from the inflorescence and then blow them into a stoppered collection tube. This method has the following disadvantages: First, it is time-consuming; second, it disturbs the fruit flies, causing them to escape and reducing the sample collection volume; third, it is not conducive to long-distance transportation of fruit flies, as the fruit flies may die during sample transportation, thus greatly reducing the sample collection efficiency; fourth, when using a spirochete to suck the fruit flies from the inflorescence, some pollen is inhaled, which is detrimental to the health of the sample collector. Summary of the Invention

[0003] The main objective of this invention is to provide a device and method for collecting, transporting, and temporarily holding taro fruit flies, in order to solve the problems existing in the prior art.

[0004] To achieve the above objectives, the present invention adopts the following technical solution:

[0005] A device for collecting, transporting, and temporarily holding taro fruit flies includes a storage box. A carrying strap is fixedly installed on the side wall of the storage box. Ventilation holes are provided on the top and side walls of the storage box. A fixing bracket assembly is provided on the inner wall of the storage box. A fixing plate is placed on the fixing bracket assembly. The fixing plate has multiple first fixing holes. A holding tube is inserted into the first fixing holes. Water-absorbing resin is laid flat on the bottom of the storage box. Both ends of the holding tube are open. The top opening of the holding tube is covered with a mesh. The bottom of the holding tube is located above the water-absorbing resin, and a mesh is provided at the bottom opening.

[0006] Furthermore, the fixing plate is provided with a limiting groove, and an ice box is installed in the limiting groove. The limiting groove is arranged along the length direction of the fixing plate and is located in the middle part of the fixing plate. The two rows of first fixing holes are respectively located on both sides of the limiting groove.

[0007] Furthermore, the storage box includes a bottom plate, side plates are fixedly provided at both ends of the bottom plate, a front plate and a rear plate are fixedly provided on both sides of the bottom plate, a top plate is connected to the top of the rear plate, one end of the top plate is connected to the rear plate by a hinge, and the other end is connected to the front plate by a latch.

[0008] Furthermore, each of the two side panels is provided with a handle fixing block on its outer side wall, and the two ends of the handle are fixed in the second fixing hole on the handle fixing block.

[0009] Furthermore, the fixed corner code assembly includes a first fixed corner code assembly and a second fixed corner code assembly. The bottom of the first fixed corner code assembly is 50mm away from the base plate, and the bottom of the second fixed corner code assembly is 150mm away from the base plate. Both the first and second fixed corner code assemblies include four fixed triangular corner codes, which are respectively located at the four corners inside the storage box.

[0010] Furthermore, a support triangle bracket is provided on the side of the side panel near the front panel, which is used to provide support for the top panel when it is closed.

[0011] Furthermore, an annular protrusion is provided on the outer wall of the holding tube at a distance of 160mm from the top, and the bottom surface of the annular protrusion is in contact with the top surface of the fixing plate.

[0012] A method for using a collection, transportation, and temporary rearing device for taro fruit flies includes the following steps:

[0013] Step 1: Place the fully absorbed water-absorbing resin at the bottom of the storage box, spreading it evenly to a thickness of 3cm;

[0014] Step 2: Take out the holding tube from the storage box, put the selected host plant containing taro fruit flies into the holding tube through the opening at the bottom of the holding tube until the top of the host plant touches the mesh, cut the stem of the host plant along the opening at the bottom of the holding tube, and then quickly tie the bottom mesh tightly with wire to ensure that the fruit flies cannot escape. Then put the holding tube containing the fruit flies and their host plant into the storage box to complete the collection of taro fruit flies.

[0015] Step 3: Repeat step 2 to collect data for all eight collection tubes. Place the collected collection tubes into the first fixing hole on the fixing plate. If the temperature is too high, place the ice box into the limiting groove inside the storage box. During the collection, transportation and temporary storage process, check the moisture content of the absorbent resin at the bottom of the storage box regularly and replenish it in time.

[0016] Furthermore, before step one, the device needs to be assembled. Take out the base plate and place it on a horizontal table. Align the end edges of the two side plates with the base plate and make them perpendicular. Then apply glue to the joint. After it is stable, proceed with the next operation. Take out the front plate and the back plate and align them with the edges of the side plates. Apply glue to the joint. Use hinges to connect the back plate and the top plate. Install the latches to connect the front plate and the top plate in appropriate positions. Install the supporting triangular brackets 285.5mm away from the base plate inside the storage box. Install the fixing triangular brackets 50mm and 150mm away from the base plate inside the storage box. Then place the fixing plate on the fixing triangular brackets. Then place the holding tube on the fixing plate. This completes the assembly of the storage box.

[0017] Compared with the prior art, the present invention has the following beneficial effects:

[0018] (1) By setting up the holding tube, the taro fruit fly and the host plant can be collected quickly, which can effectively improve the collection efficiency, prevent the host and taro fruit fly from being squeezed during transportation, and greatly reduce escape.

[0019] (2) The mesh on the top of the container, the ventilation holes in the storage box, and the ice box can ensure air circulation and suitable temperature during transportation, which are all conducive to the survival of the sample.

[0020] (3) By placing water-absorbing resin at the bottom of the box to provide water for the host plant, the normal state of the host can be extended for a certain period of time, providing living conditions for the taro fruit fly and avoiding the death of the taro fruit fly;

[0021] (4) The method of collecting taro fruit flies along with the host plant also avoids the health impact on the collector when directly sucking fruit flies from the inflorescence. Attached Figure Description

[0022] Figure 1 This is a schematic diagram of the overall structure of the present invention.

[0023] Figure 2 This is a schematic diagram of the storage box in the closed state of the present invention.

[0024] Figure 3 This is a schematic diagram of the internal structure of the storage box of the present invention.

[0025] Figure 4 This is a schematic diagram of the holding tube structure of the present invention.

[0026] Figure 5 This is a schematic diagram of the fixed card plate structure of the present invention.

[0027] Among them, 1-storage box, 2-containment tube, 3-fixing plate, 4-handle, 5-ice box, 6-supporting triangular bracket, 11-top plate, 12-front plate, 13-hinge, 14-second fixing hole, 15-side plate, 16-locking movable part, 17-locking fixed part, 18-fixing triangular bracket, 19-bottom plate, 21-mesh, 22-annular protrusion, 31-first fixing hole, 32-limiting groove. Detailed Implementation

[0028] The technical solution of the present invention will be further described below with reference to the accompanying drawings and embodiments.

[0029] Combination Figures 1 to 5 This invention provides a device for collecting, transporting, and temporarily holding taro fruit flies, including a storage box 1. A carrying strap 4 is fixedly installed on the side wall of the storage box 1. Ventilation holes are provided on the top and side walls of the storage box 1. A fixing bracket assembly is provided on the inner wall of the storage box 1. A fixing plate 3 is placed on the fixing bracket assembly. The fixing plate 3 is provided with a plurality of first fixing holes 31. A holding tube 2 is inserted into the first fixing holes 31. Water-absorbing resin is laid flat at the bottom of the storage box 1. The holding tube 2 has openings at both ends. The top opening of the holding tube 2 is covered with a mesh 21. The bottom of the holding tube 2 is located above the water-absorbing resin, and a mesh is provided at the bottom opening.

[0030] Preferably, the fixing plate 3 is made of an acrylic sheet with a length of 315mm, a width of 180mm and a thickness of 4.5mm, and has 8 first fixing holes 31 distributed on it, each with a diameter of 60mm.

[0031] In this embodiment, the fixing plate 3 is provided with a limiting groove 32, and an ice box 5 is installed in the limiting groove 32. The limiting groove 32 is arranged along the length direction of the fixing plate 3 and is located in the middle part of the fixing plate 3. The two rows of first fixing holes 31 are respectively located on both sides of the limiting groove 32. Preferably, the length, width and height of the ice box are 245mm, 15mm and 290mm respectively. Depending on the weather conditions, the ice box 5 can be installed in the limiting groove 32 in the storage box 1 to ensure that the temperature in the storage box 1 is suitable. Before use, water is added to the ice box and it is placed in the refrigerator for freezing and cold storage.

[0032] In this embodiment, the storage box 1 includes a bottom plate 19, with side plates 15 fixedly disposed at both ends of the bottom plate 19. A front plate 12 and a rear plate are fixedly disposed on both sides of the bottom plate 19. A top plate 11 is connected to the top of the rear plate. One end of the top plate 11 is connected to the rear plate via a hinge 13, and the other end is connected to the front plate 12 via a latch. Preferably, the latch includes a latch fixing part 17 and a latch movable part 16. The latch fixing part 17 is glued to the middle of the front plate 12, and the latch movable part 16 is glued to the corresponding position of the top plate. The two cooperate to lock and fix the top plate.

[0033] Preferably, the top plate, front plate, rear plate, bottom plate, and side plates are all made of acrylic sheet material. The top plate is 315mm long, 180mm wide, and 4.5mm thick, and has a 7-row, 15-column ventilation hole matrix with a row and column spacing of 20mm and each ventilation hole diameter of 10mm. The front and rear plates have the same dimensions, 324.2mm long, 290mm wide, and 4.5mm thick, with a 4-row, 14-column ventilation hole matrix at the top, a row and column spacing of 20mm and each ventilation hole diameter of 10mm. The bottom plate is 315mm long, 180mm wide, and 4.5mm thick. The side plates are 290mm long, 180mm wide, and 4.5mm thick, with a 4-row, 7-column ventilation hole matrix at the top, a row and column spacing of 20mm and each ventilation hole diameter of 10mm.

[0034] In this embodiment, each of the two side plates 15 has a handle fixing block on its outer side wall, and both ends of the handle 4 are fixed in the second fixing hole 14 on the handle fixing block. Preferably, the handle is a nylon braided strap that is 2m long and 50mm wide.

[0035] In this embodiment, the fixed corner bracket assembly includes a first fixed corner bracket assembly and a second fixed corner bracket assembly. The bottom of the first fixed corner bracket assembly is 50mm away from the base plate, and the bottom of the second fixed corner bracket assembly is 150mm away from the base plate. Both the first and second fixed corner bracket assemblies include four fixed triangular corner brackets 18, which are respectively located at the four corners inside the storage box 1. The four fixed triangular corner brackets 18 form a group to provide support for the fixed card plate.

[0036] In this embodiment, the side plate 15 is provided with a supporting triangular bracket 6 on the side near the front plate 12. The supporting triangular bracket 6 is used to provide support for the top plate 11 in the closed state. The bottom of the supporting triangular bracket 6 is 285.5mm away from the bottom plate.

[0037] In this embodiment, an annular protrusion 22 is provided on the outer wall of the holding tube 2 at a distance of 160mm from the top, and the bottom surface of the annular protrusion is in contact with the top surface of the fixing plate 3. Preferably, the holding tube is made of 250mm thick acrylic material with an outer diameter of 60mm, a wall thickness of 2mm, and an inner diameter of 56mm. A 100-mesh mesh is glued to the top for ventilation, and the bottom opening is for placing the host plant. The annular protrusion is used to limit the distance between the bottom opening of the holding tube and the bottom plate to prevent the host plant from being squeezed during transportation.

[0038] Example 2

[0039] A method for using a collection, transportation, and temporary rearing device for taro fruit flies includes the following steps:

[0040] Step 1: Place the fully absorbed water-absorbing resin at the bottom of storage box 1, spreading it evenly to a thickness of 3cm;

[0041] Step 2: Remove the holding tube 2 from storage box 1. Place the selected host plant containing taro fruit flies into the holding tube 2 through the opening at the bottom until the top of the host plant touches the mesh 21. Cut the stem of the host plant along the opening at the bottom of the holding tube 2. Then quickly tie the bottom mesh tightly with wire to ensure that the fruit flies cannot escape. Place the holding tube 2 containing the fruit flies and their host plant back into storage box 1 to complete the collection of taro fruit flies. Preferably, the stem of the host plant is in contact with the water-absorbing resin, which facilitates the transfer of water to the stem of the host plant, providing water to the host plant and prolonging the normal state of the host for a certain period of time, providing survival conditions for the taro fruit flies and preventing their death.

[0042] Step 3: Repeat step 2 to collect data and complete the collection of eight holding tubes 2. Place the collected holding tubes 2 into the first fixing hole 31 on the fixing plate 3. If the temperature is too high, place the ice box 5 into the limiting groove 32 in the storage box 1. During the collection, transportation and temporary storage process, check the water content of the water-absorbing resin at the bottom of the storage box 1 regularly and replenish it in time.

[0043] In this embodiment, the device needs to be assembled before step one. Take out the base plate 19 and place it on a horizontal table. Align the end edges of the two side plates 15 with the base plate 19 and make them perpendicular. Then apply glue to the joint. After it is stable, proceed with the next operation. Take out the front plate 12 and the rear plate and align them with the edges of the side plates 15. Apply glue to the joint. Use the hinge 13 to connect the rear plate and the top plate 11. Install the latch to connect the front plate 12 and the top plate 11 at the appropriate position. Install the supporting triangular bracket 6 inside the storage box 1 at a distance of 19285.5mm from the base plate. Install the fixing triangular bracket 18 inside the storage box 1 at a distance of 1950mm and 150mm from the base plate. Then place the fixing plate 3 on the fixing triangular bracket 18. Then place the holding tube 2 on the fixing plate 3. The assembly of the storage box 1 is now complete.

[0044] Test insect source and collection efficiency experiment: The test insect source was randomly selected from the fruit flies on the inflorescence of Taroa alopecuroides in Laoyuhe Wetland Park, and collected using a suction tube and this device.

[0045] Two collection devices were used to conduct collection experiments. Three inflorescences were randomly selected for the experiments, and each method was repeated three times. The collection time for each device and the number of taro fruit flies that escaped during collection were recorded (Table 1). Table 2 shows the comparison of collection efficiency of different collection methods.

[0046] Table 1

[0047]

[0048] Table 2

[0049]

[0050] Note: Data collection time and escape ratio in the table are mean ± standard error. Identical lowercase letters indicate no significant difference, while different lowercase letters indicate a significant difference. Significance was calculated using DPS 7.5.

[0051] The collection time and escape rate of the present invention are significantly different from those of the trematode tube. The collection time of the present invention is significantly shorter than that of the trematode tube (P<0.05), and the escape rate is also significantly lower than that of the trematode tube (P<0.01).

[0052] The above test results show that the device used in this invention is significantly superior to the insect suction tube.

[0053] The above description is merely a preferred embodiment of the present invention and does not constitute any limitation on the technical scope of the present invention. Therefore, any minor modifications, equivalent changes and alterations made to the above embodiments based on the technical essence of the present invention shall still fall within the scope of the technical solution of the present invention.

Claims

1. A device for collecting, transporting, and temporarily holding taro fruit flies, characterized in that, The storage box includes a carrying strap fixedly installed on its side wall, ventilation holes on its top and side walls, a fixing bracket assembly on its inner wall, a fixing plate on the fixing bracket assembly, a plurality of first fixing holes on the fixing plate, a holding tube being inserted into the first fixing holes, absorbent resin being laid flat on the bottom of the storage box, the holding tube having openings at both ends, a mesh covering the top opening of the holding tube, and the bottom of the holding tube being above the absorbent resin, with a mesh covering the bottom opening. The fixing plate is provided with a limiting groove, and an ice box is installed in the limiting groove. The limiting groove is arranged along the length direction of the fixing plate and is located in the middle part of the fixing plate. The two rows of first fixing holes are respectively located on both sides of the limiting groove. The storage box includes a bottom plate, side plates are fixedly provided at both ends of the bottom plate, a front plate and a rear plate are fixedly provided on both sides of the bottom plate, a top plate is connected to the top of the rear plate, one end of the top plate is connected to the rear plate by a hinge, and the other end is connected to the front plate by a latch. Each of the two side panels has a handle fixing block on its outer side wall, and the two ends of the handle are fixed in the second fixing hole on the handle fixing block; The fixed corner code assembly includes a first fixed corner code assembly and a second fixed corner code assembly. The bottom of the first fixed corner code assembly is 50mm away from the bottom plate, and the bottom of the second fixed corner code assembly is 150mm away from the bottom plate. Both the first fixed corner code assembly and the second fixed corner code assembly include four fixed triangular corner codes, which are respectively located at the four corners inside the storage box. The side panel is provided with a support triangle bracket on the side near the front panel, and the support triangle bracket is used to provide support for the top panel when it is closed.

2. The collection, transportation, and temporary rearing device for taro fruit flies as described in claim 1, characterized in that, An annular protrusion is provided on the outer wall of the holding tube at a distance of 160mm from the top, and the bottom surface of the annular protrusion is in contact with the top surface of the fixing plate.

3. The method of using the collection, transportation, and temporary rearing device for taro fruit flies as described in any one of claims 1-2, characterized in that, Includes the following steps: Step 1: Place the fully absorbed water-absorbing resin at the bottom of the storage box, spreading it evenly to a thickness of 3cm; Step 2: Take out the holding tube from the storage box, put the selected host plant containing taro fruit flies into the holding tube through the opening at the bottom of the holding tube until the top of the host plant touches the mesh, cut the stem of the host plant along the opening at the bottom of the holding tube, and then quickly tie the bottom mesh tightly with wire or rope to ensure that the fruit flies cannot escape. Then put the holding tube containing the fruit flies and their host plants into the storage box to complete the collection of taro fruit flies. Step 3: Repeat step 2 to collect data for all eight collection tubes. Place the collected collection tubes into the first fixing hole on the fixing plate. If the temperature is too high, place the ice box into the limiting groove inside the storage box. During the collection, transportation and temporary storage process, check the moisture content of the absorbent resin at the bottom of the storage box regularly and replenish it in time.

4. The method of using the collection, transportation, and temporary rearing device for taro fruit flies as described in claim 3, characterized in that, Before step one, the device needs to be assembled. Take out the base plate and place it on a horizontal table. Align the ends of the two side plates with the base plate and make them perpendicular. Then apply glue to the joint. After it is stable, proceed with the next operation. Take out the front plate and the back plate and align them with the edges of the side plates. Apply glue to the joint. Use hinges to connect the back plate and the top plate. Install the latches to connect the front plate and the top plate in appropriate positions. Install the supporting triangular brackets 285.5mm away from the base plate inside the storage box. Install the fixing triangular brackets 50mm and 150mm away from the base plate inside the storage box. Then place the fixing plate on the fixing triangular brackets. Then place the holding tube on the fixing plate. This completes the assembly of the storage box.

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