A method for artificially raising the entire life cycle of the spotted bug.

Abstract

This invention belongs to the field of insect rearing technology, specifically relating to a method for artificially rearing the entire life cycle of the spotted bug (Aegilops spp.), comprising the following steps: S1, placing spotted bug eggs in a culture dish and transferring them to an intelligent artificial climate chamber until the nymphs hatch; S2, transferring the spotted bug nymphs from the intelligent artificial climate chamber to a rearing cage, and feeding them a full-life-cycle feed daily; S3, after the adults emerge, pairing the male and female adults, and feeding them a full-life-cycle feed daily, transferring the eggs to a rearing tube after the female lays eggs. The full-life-cycle feed for spotted bugs is composed of the following components by mass fraction: 90%-95% legume feed and 5%-10% egg yolk protein-loaded enzyme preparation. This method avoids contamination from spotted bug excrement and other impurities, reduces the probability of mutation and mortality, and improves the efficiency of nutrient absorption by spotted bugs.

Description

Technical Field

[0001] This invention belongs to the field of insect breeding technology, specifically relating to a method for artificially breeding the entire life cycle of the spotted bug. Background Technology

[0002] The spotted stink bug (Periplaneta americana) belongs to the family Periplanetacidae in the order Hemiptera. With a wide host range, it has become a significant pest of soybean production in my country in recent years, being one of the main factors causing "greening" in soybeans in the Huang-Huai-Hai region, severely impacting soybean yield and quality. Adults are approximately 14-17 mm in length, yellowish-brown to dark brown in color, with a triangular head, spiny sides of the pronotum, and a narrower anterior abdomen. The first antennal segment is longer than the second, and the fourth segment is longer than the sum of the second and third segments. In northern regions such as Beijing and Hebei, the spotted stink bug has three generations per year, overwintering as adults. Adults emerge in April of the following year when the host plant turns green. Mating and egg-laying occur from May to July, with eggs mostly laid on the undersides of leaves, tender shoots, and young stems. Mature nymphs emerge as adults in July and August, continuing to mate and lay eggs. The second generation of adults emerges around August to September, and the third generation appears in October and November, entering the overwintering period. Artificial breeding sites for the bee-like bug can help us better understand its biological characteristics and provide a stable and consistent standard source of experimental insects for related research, thus supporting its green prevention and control to ensure safe soybean production.

[0003] In the current technology, the artificial rearing of the Spotted Beetle still lacks a relatively systematic method for the entire life cycle. Most artificial rearing of the Spotted Beetle only focuses on the adult stage, and the feed is mostly fresh bean pods, which are not easy to obtain and preserve for a long time, resulting in poor rearing effect. Summary of the Invention

[0004] The purpose of this invention is to provide a method for artificially raising the entire life cycle of the spotted bug, from egg to nymph hatching, nymph development into adult, adult emergence, and female egg laying.

[0005] The technical problem to be solved by this invention is: a method for raising animals without a systematic approach throughout their entire reproductive life.

[0006] The objective of this invention can be achieved through the following technical solutions:

[0007] A method for artificially raising the entire life cycle of the Spotted Bug, comprising the following steps:

[0008] S1. Place 200 eggs of the spotted bee bug into a petri dish, and then transfer the petri dish to an intelligent artificial climate chamber, controlling the temperature at 25℃-26℃, the relative humidity at 75%-80%, and the light time at 16h-17h until the nymphs hatch.

[0009] S2. Transfer the nymphs of the spotted bug from the intelligent artificial climate chamber to the insect rearing tank. Adjust the temperature of the intelligent artificial climate chamber to 25℃-26℃, the relative humidity to 75%-80%, and the light time to 16h-17h. Feed the spotted bugs with feed for the entire life cycle at regular intervals every day, and clean up the spotted bugs' excrement, molts, and old feed in a timely manner.

[0010] S3. After 16-20 days of rearing, the adults will emerge. Pair the male and female adults and feed them with the full life cycle feed of the bee-like bugs at regular intervals every day. Clean up the debris in time. After 3-5 days, the female bugs will lay eggs and transfer the eggs to a culture dish.

[0011] In step S1, the petri dish is made of glass, with a diameter of 9cm and a height of 20mm.

[0012] In step S2, the insect tank is made of glass, with a diameter of 15cm and a height of 20cm.

[0013] In step S2, the feed for the entire life cycle of the bee-like bug is composed of the following components by mass fraction: 90%-95% legume feed and 5%-10% egg yolk protein loaded with enzyme preparation.

[0014] Furthermore, the legume feed is prepared by the following steps:

[0015] Select 50g-60g of soybeans or mung beans, remove bad beans, and use them as legume feed.

[0016] The enzyme preparation refers to one or more of amylase, lipase, and protease.

[0017] Furthermore, the feed for the entire life cycle of the spotted bug is prepared by the following steps:

[0018] A1. Place the egg yolk in a centrifuge, add phosphate buffer, and centrifuge at 500 rpm-600 rpm to obtain egg yolk protein precipitate. Wash with distilled water, concentrate, mix the enzyme preparation with the concentrated egg yolk protein, stir, and control the temperature at 33℃-35℃ to obtain egg yolk protein loaded with enzyme preparation.

[0019] A2. Add egg yolk protein loaded with enzyme preparation and legume feed into a mixer and mix thoroughly to obtain feed for the entire growth period of the spotted bug.

[0020] In step A1, the ratio of egg yolk, phosphate buffer, and enzyme preparation is (10-15) g : (20-30) mL : (1-3) g.

[0021] The beneficial effects of this invention are:

[0022] In this invention, the egg yolk protein-loaded enzyme preparation enhances enzyme stability and activity. The egg yolk protein, acting as a carrier, protects the enzyme from adverse environmental factors while providing a suitable environment for enzyme catalysis. The egg yolk protein strengthens enzyme stability, allowing it to maintain activity over a wide temperature and pH range. It also prevents contamination from the excrement of the spotted bug, reducing the likelihood of mutation and death in the bug.

[0023] In the technical solution of this invention, the yolk protein can be completely broken down to produce amino acids, supplementing the essential amino acid intake of the bee bug. Detailed Implementation

[0024] The technical solutions of the present invention will be clearly and completely described below with reference to the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of the present invention. Example

[0025] The feed for the entire life cycle of the Spotted Bee Bug is prepared through the following steps:

[0026] A1. Place 10g of egg yolk in a centrifuge, add 20mL of phosphate buffer, centrifuge at 500rpm-600rpm to obtain egg yolk protein precipitate, wash with distilled water, concentrate, mix 1g of enzyme preparation with the concentrated egg yolk protein, stir, and control the temperature at 33℃-35℃ to obtain egg yolk protein loaded with enzyme preparation.

[0027] A2. Add egg yolk protein loaded with enzyme preparation and legume feed into a mixer and mix thoroughly to obtain feed for the entire growth period of the spotted bug. Example

[0028] The feed for the entire life cycle of the Spotted Bee Bug is prepared through the following steps:

[0029] A1. Place 12.5g of egg yolk into a centrifuge, add 25mL of phosphate buffer, centrifuge at 500rpm-600rpm to obtain egg yolk protein precipitate, wash with distilled water, concentrate, mix 2g of enzyme preparation with the concentrated egg yolk protein, stir, control the temperature at 33℃-35℃, and obtain egg yolk protein loaded with enzyme preparation.

[0030] A2. Add egg yolk protein loaded with enzyme preparation and legume feed into a mixer and mix thoroughly to obtain feed for the entire growth period of the spotted bug. Example

[0031] The feed for the entire life cycle of the Spotted Bee Bug is prepared through the following steps:

[0032] A1. Place 15g of egg yolk in a centrifuge, add 30mL of phosphate buffer, centrifuge at 500rpm-600rpm to obtain egg yolk protein precipitate, wash with distilled water, concentrate, mix 3g of enzyme preparation with the concentrated egg yolk protein, stir, and control the temperature at 33℃-35℃ to obtain egg yolk protein loaded with enzyme preparation.

[0033] A2. Add egg yolk protein loaded with enzyme preparation and legume feed into a mixer and mix thoroughly to obtain feed for the entire growth period of the spotted bug. Example

[0034] A method for artificially raising the entire life cycle of the Spotted Bug, comprising the following steps:

[0035] S1. Place 200 eggs of the spotted bug in a petri dish, then transfer the petri dish to an intelligent artificial climate chamber, and control the temperature at 27℃-28℃, the relative humidity at 75%-85%, and the light time at 16h-17h until the nymphs hatch.

[0036] S2. Transfer the nymphs of the spotted bug to the rearing tank in the intelligent artificial climate chamber. Adjust the temperature of the intelligent artificial climate chamber to 25℃-26℃, the relative humidity to 75%-80%, and the light time to 16h-17h. Feed the spotted bug with the full life cycle feed and water prepared in Example 1 at regular intervals every day, and clean up the spotted bug excrement, molts and old feed in a timely manner.

[0037] S3. After 16-20 days of rearing, the adults emerge. The male and female adults are paired up, and 20 pairs are raised in single pairs. The adult beetles are fed the full-life-cycle feed and water prepared in Example 1 at regular intervals every day. Debris is cleaned up in time. After 3-5 days, the female lays eggs and the eggs are transferred to a culture dish. Example

[0038] A method for artificially raising the entire life cycle of the Spotted Bug, comprising the following steps:

[0039] S1. Place 200 eggs of the spotted bee bug into a petri dish, and then transfer the petri dish to an intelligent artificial climate chamber, controlling the temperature at 25℃-26℃, the relative humidity at 75%-80%, and the light time at 16h-17h until the nymphs hatch.

[0040] S2. Transfer the nymphs of the spotted bug to the rearing tank in the intelligent artificial climate chamber. Adjust the temperature of the intelligent artificial climate chamber to 25℃-26℃, the relative humidity to 75%-80%, and the light time to 16h-17h. Feed the spotted bug with the full life cycle feed and water prepared in Example 2 at regular intervals every day, and clean up the spotted bug excrement, molts and old feed in a timely manner.

[0041] S3. After 16-20 days of rearing, the adults emerge. The male and female adults are paired up, and 20 pairs are raised individually. The adult beetles are fed the full-life-cycle feed and water prepared in Example 2 at regular intervals every day. Debris is cleaned up in time. After 3-5 days, the female lays eggs and the eggs are transferred to a culture dish. Example

[0042] A method for artificially raising the entire life cycle of the Spotted Bug, comprising the following steps:

[0043] S1. Place 200 eggs of the spotted bee bug into a petri dish, and then transfer the petri dish to an intelligent artificial climate chamber, controlling the temperature at 25℃-26℃, the relative humidity at 75%-80%, and the light time at 16h-17h until the nymphs hatch.

[0044] S2. Transfer the nymphs of the spotted bug to the rearing tank in the intelligent artificial climate chamber. Adjust the temperature of the intelligent artificial climate chamber to 25℃-26℃, the relative humidity to 75%-80%, and the light time to 16h-17h. Feed the spotted bug with the full life cycle feed and water prepared in Example 3 at regular intervals every day, and clean up the spotted bug excrement, molts and old feed in a timely manner.

[0045] S3. After 16-20 days of rearing, the adults emerge. The male and female adults are paired up, and 20 pairs are raised in single pairs. The adult beetles are fed the full-life-cycle feed and water prepared in Example 3 at regular intervals every day. Debris is cleaned up in time. After 3-5 days, the female lays eggs and the eggs are transferred to a culture dish.

[0046] S1. Place 200 eggs of the spotted bee bug into a petri dish, and then transfer the petri dish to an intelligent artificial climate chamber, controlling the temperature at 25℃-26℃, the relative humidity at 75%-80% and the light time at 16h-17h until the nymphs hatch.

[0047] S2. Transfer the nymphs of the Spotted Bee Bug to the rearing tank in the intelligent artificial climate chamber. Adjust the temperature of the intelligent artificial climate chamber to 25℃-26℃, the relative humidity to 75%-80%, and the light time to 16h-17h. Feed them fresh bean pods at regular intervals every day and clean up the excrement, molts and old feed of the Spotted Bee Bug in a timely manner.

[0048] S3. After 18-28 days of rearing, the adults will emerge. Pair the male and female adults and rear 20 pairs at a time. Feed them fresh pods regularly every day and clean up debris in time. After 5-9 days, the female will lay eggs and transfer the eggs to a petri dish.

[0049] Following the rearing methods of Examples 4-6 and Comparative Example 1, 200 eggs of *Aegilops spp.* were reared until 30 days after emergence. Detailed information about the entire life cycle of the 200 eggs was recorded, and the hatching rate, adult emergence rate, and female oviposition rate were calculated. The results are shown in Tables 1 and 2.

[0050]

[0051] As shown in Tables 1 and 2, the hatching rate of eggs and the emergence rate of adults in Examples 4-6 were higher than those in Comparative Example 1. This indicates that feeding the bug with feed for the entire life cycle of the bug can provide sufficient nutrition for the bug while avoiding contamination from excrement and other impurities, thus effectively reducing the mortality rate of the bug.

[0052] In the description of this specification, the references to terms such as "an embodiment," "example," "specific example," etc., indicate that a specific feature, structure, material, or characteristic described in connection with that embodiment or example is included in at least one embodiment or example of the present invention. In this specification, the illustrative expressions of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples.

[0053] The above description is merely an example and illustration of the present invention. Those skilled in the art can make various modifications or additions to the specific embodiments described, or use similar methods to replace them, as long as they do not deviate from the invention or exceed the scope defined in the claims, all of which should fall within the protection scope of the present invention.

Claims

1. A method for artificially raising the entire life cycle of the spotted bug, characterized in that, Includes the following steps: S1. Place 200 eggs of the spotted bee bug into a petri dish, and then transfer the petri dish to an intelligent artificial climate chamber, controlling the temperature at 25℃-26℃, the relative humidity at 75%-80%, and the light time at 16h-17h until the nymphs hatch. S2. Transfer the nymphs of the spotted bug from the intelligent artificial climate chamber to the insect rearing tank. Adjust the temperature of the intelligent artificial climate chamber to 25℃-26℃, the relative humidity to 75%-80%, and the light time to 16h-17h. Feed the spotted bugs with feed for the entire life cycle at regular intervals every day, and clean up the spotted bugs' excrement, molts, and old feed in a timely manner. The feed for the entire life cycle of the horned bug in step S2 is composed of the following components by mass fraction: 90%-95% legume feed and 5%-10% egg yolk protein loaded with enzyme preparation; S3. After 16-20 days of rearing, the adults will emerge. Pair the male and female adults and feed them with the full life cycle feed of the bee-margined bug at regular intervals every day. Clean up the debris in time. After 3-5 days, the female will lay eggs and transfer the eggs to a petri dish. The feed for the entire life cycle of the Spotted Bee Bug is prepared through the following steps: A1. Place the egg yolk in a centrifuge, add phosphate buffer, and centrifuge at 500 rpm-600 rpm to obtain egg yolk protein precipitate. Wash with distilled water, concentrate, mix the enzyme preparation with the concentrated egg yolk protein, stir, and control the temperature at 33℃-35℃ to obtain egg yolk protein loaded with enzyme preparation. A2. Add egg yolk protein loaded with enzyme preparation and legume feed into a mixer and mix thoroughly to obtain feed for the entire growth period of the spotted bug.

2. The method for artificially raising the entire life cycle of the spotted bug according to claim 1, characterized in that, In step S1, the petri dish is made of glass, with a diameter of 9cm and a height of 20mm.

3. The method for artificially raising the entire life cycle of the spotted bug according to claim 2, characterized in that, In step S2, the insect tank is made of glass, with a diameter of 15cm and a height of 20cm.

4. The method for artificially raising the entire life cycle of the spotted bug according to claim 3, characterized in that, Legume feed is prepared by the following steps: Select 50g-60g of soybeans or mung beans, remove bad beans, and use them as legume feed.

5. A method for artificially raising the entire life cycle of the spotted bug according to claim 4, characterized in that, Enzyme preparations refer to one or more of amylase, lipase, and protease.

6. A method for artificially raising the entire life cycle of the spotted bug according to claim 5, characterized in that, In step A1, the ratio of egg yolk, phosphate buffer, and enzyme preparation is (10-15) g : (20-30) mL : (1-3) g.

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