Method for simultaneous removal of polycyclic aromatic hydrocarbons and heavy metals and use thereof

Abstract

The application discloses a method and application for simultaneously removing polycyclic aromatic hydrocarbons and heavy metals, and comprises the following steps: inoculating Pseudomonas stutzeri MSC14 into pollutants containing heavy metals and polycyclic aromatic hydrocarbons, and removing the polycyclic aromatic hydrocarbons and heavy metals after culture. The pollutants containing heavy metals and polycyclic aromatic hydrocarbons are treated by using Pseudomonas stutzeri MSC14, so that the polycyclic aromatic hydrocarbons and heavy metals can be simultaneously removed; meanwhile, the heavy metals can promote the degradation efficiency of Pseudomonas stutzeri MSC14 at a certain concentration, and the repair effect of the pollutants of heavy metals and / or polycyclic aromatic hydrocarbons is obviously improved.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of microbial remediation of pollutants, and particularly relates to a method for simultaneously removing polycyclic aromatic hydrocarbons and heavy metals and application thereof. BACKGROUND

[0002] Polycyclic aromatic hydrocarbons and heavy metals are typical representatives of organic and inorganic substances entering the environment. Investigation and research shows that more than 40% of contaminated sites are polycyclic aromatic hydrocarbon and heavy metal combined pollution (Sandrin T R, Maier R M. Impact of metals on the biodegradation of organic pollutants [J]. ENVIRONMENTAL HEALTH PERSPECTIVES, 2003, 111 (8): 1093-1101.). Due to their high toxicity and high persistence, the remediation of combined pollution sites has attracted much attention. Compared with physical and chemical remediation technologies, biological remediation is considered to be a safer, cleaner and more efficient remediation strategy. However, heavy metal stress can have adverse effects on organisms, thereby affecting the biodegradation of polycyclic aromatic hydrocarbons. Combined pollution of polycyclic aromatic hydrocarbons and heavy metals mainly affects the biodegradation of polycyclic aromatic hydrocarbons and the transmembrane transport of heavy metals (Liu S, Zeng G, Niu Q, et al. Bioremediation mechanisms of combined pollution of PAHs and heavy metals by bacteria and fungi: A mini review [J]. BIORESOURCE TECHNOLOGY, 2017, 224: 25-33.). The permeability of the cell membrane of organisms subjected to double poisoning changes significantly, and the cell membrane is severely damaged, so a large number of studies have reported that combined pollution of polycyclic aromatic hydrocarbons and heavy metals is more toxic than single pollutants (Ali N, Bilal M, Khan A, et al. Effective exploitation of anionic, nonionic, and nanoparticle-stabilized surfactant foams for petroleum hydrocarbon contaminated soil remediation [J]. SCIENCE OF THE TOTAL ENVIRONMENT, 2020, 704.), and the difficulty of biological remediation of combined pollution sites is significantly increased.

[0003] Therefore, providing a method for simultaneously removing polycyclic aromatic hydrocarbons and heavy metal pollutants is of great help to the bioremediation of heavy metal and polycyclic aromatic hydrocarbon composite contaminated areas. SUMMARY

[0004] The present application aims to overcome the shortcomings of the prior art and provide a method for simultaneously removing polycyclic aromatic hydrocarbons and heavy metals and an application.

[0005] In order to achieve the above-mentioned purpose, the technical scheme provided by the present application is as follows:

[0006] A method for simultaneously removing polycyclic aromatic hydrocarbons and heavy metals, comprising the following steps:

[0007] Inoculating Pantoea dispersa MSC14 into a pollutant containing heavy metals and polycyclic aromatic hydrocarbons, and removing polycyclic aromatic hydrocarbons and heavy metals after cultivation.

[0008] Preferably, the Pantoea dispersa MSC14 has a preservation number of GDMCC No:62680. It was preserved on August 3, 2022, at the Guangdong Microbial Culture Collection Center located at No. 100, Xianlie Middle Road, Guangzhou City, Guangdong Province, 5th Floor, Building 59 (Patent Application Publication No: CN 115820494A).

[0009] Preferably, the heavy metal includes at least one of copper, nickel, lead, and cadmium (Cu, Ni, Pb, Cd).

[0010] Preferably, the polycyclic aromatic hydrocarbon includes at least one of benz[a]pyrene, pyrene, fluoranthene, phenanthrene, fluorene, and anthracene.

[0011] Preferably, the pollutant is a water body or soil.

[0012] Preferably, the pollutant is a water body, and the concentration of the heavy metal is (0, 300] mg / L (greater than 0 and less than 300 mg / L). In this concentration range, the heavy metal has a promoting or less inhibiting effect on the degradation of polycyclic aromatic hydrocarbons by Pantoea dispersa MSC14.

[0013] Further preferably, the pollutant is a water body, and the concentration of the heavy metal is (0, 100] mg / L.

[0014] More preferably, the pollutant is a water body, and the heavy metal is copper, and the concentration of the copper is (0, 300] mg / L.

[0015] More preferably, the pollutant is a water body, and the heavy metal is nickel, and the concentration of the nickel is (0, 200] mg / L.

[0016] More preferably, the pollutant is water body, and the heavy metal is lead, wherein the concentration of the lead is (0, 300] mg / L.

[0017] More preferably, the pollutant is water body, and the heavy metal is cadmium, wherein the concentration of the cadmium is (0, 100] mg / L.

[0018] Further preferably, the pollutant is water body, wherein the concentration of the heavy metal is (0, 1] mg / L. In this concentration range, the heavy metal promotes the degradation of polycyclic aromatic hydrocarbons by Pseudomonas sp. MSC14.

[0019] Preferably, the pollutant is water body, wherein the concentration of the polycyclic aromatic hydrocarbon is (0, 50] mg / L.

[0020] Further preferably, the pollutant is water body, wherein the concentration of the polycyclic aromatic hydrocarbon is (0, 20] mg / L.

[0021] More preferably, the pollutant is water body, and the polycyclic aromatic hydrocarbon is benzo[a]pyrene, wherein the concentration of the benzo[a]pyrene is (0, 20] mg / L.

[0022] More preferably, the pollutant is water body, and the polycyclic aromatic hydrocarbon is phenanthrene, wherein the concentration of the phenanthrene is (0, 20] mg / L.

[0023] More preferably, the pollutant is water body, and the polycyclic aromatic hydrocarbon is fluorene, wherein the concentration of the fluorene is (0, 20] mg / L.

[0024] More preferably, the pollutant is water body, and the polycyclic aromatic hydrocarbon is fluoranthene, wherein the concentration of the fluoranthene is (0, 20] mg / L.

[0025] Preferably, the temperature of the culture is 30-35℃, and the time is 4-5 days.

[0026] The above method for simultaneously removing polycyclic aromatic hydrocarbons and heavy metals is applied to the remediation of soil and wastewater contaminated by heavy metals and / or polycyclic aromatic hydrocarbons.

[0027] Compared with the prior art, the present application has the following beneficial effects:

[0028] (1) The Pseudomonas sp. strain Pseudomonas sp. MSC14 of the present application can grow under stress of various heavy metals (copper, nickel, lead, cadmium, etc.), and is resistant to high concentrations of heavy metals; and can simultaneously remove polycyclic aromatic hydrocarbons (benzo[a]pyrene, pyrene, fluoranthene, phenanthrene, fluorene, anthracene, etc.) and heavy metals in pollutants.

[0029] (2) The heavy metal of the present application can significantly promote the removal efficiency of Pseudomonas dispersa MSC14 on polycyclic aromatic hydrocarbons in (0, 1] mg / L, especially the heavy metal lead, and the promotion effect is more optimal. BRIEF DESCRIPTION OF DRAWINGS

[0030] Figure 1 The colony morphology of the MSC14 strain of the present application in LB solid medium;

[0031] Figure 2 The degradation rate of MSC14 strain on benz[a]pyrene under different concentrations of copper stress;

[0032] Figure 3 The degradation rate of MSC14 strain on benz[a]pyrene under different concentrations of heavy metal stress;

[0033] Figure 4 The removal capacity of MSC14 strain on metals under heavy metal and benz[a]pyrene stress;

[0034] Figure 5 The degradation rate of MSC14 strain on different polycyclic aromatic hydrocarbons under copper stress. DETAILED DESCRIPTION

[0035] It should be understood that the specific embodiments described herein are merely illustrative of the present application and do not limit the present application.

[0036] Example 1: Minimum inhibitory concentration of MSC14 strain

[0037] 1.1 Gradient liquid culture method for measuring the minimum inhibitory concentration of MSC14 strain on different heavy metals

[0038] (1) Prepare LB liquid medium with the formula: 10 g / L tryptone, 10 g / L NaCl, 5 g / L yeast extract, sterilize at 121℃ for 20 min;

[0039] (2) Weigh 1.476 g of anhydrous copper nitrate on a balance and dissolve it in a suitable amount of ultrapure water, then make up to 50 mL after complete dissolution to obtain a copper nitrate stock solution;

[0040] (4) Weigh 2.239 g of nickel sulfate hexahydrate on a balance and dissolve it in a suitable amount of ultrapure water, then make up to 50 mL after complete dissolution to obtain a nickel sulfate stock solution;

[0041] (3) Weigh 0.799 g of anhydrous lead nitrate on a balance and dissolve it in a suitable amount of ultrapure water, then make up to 50 mL after complete dissolution to obtain a lead nitrate stock solution;

[0042] (5) Weigh 1.052 g of anhydrous cadmium nitrate on a balance and dissolve it in a suitable amount of ultrapure water, then make up to 50 mL after complete dissolution to obtain a cadmium nitrate stock solution;

[0043] (6) The bacterial seed liquid stored in a glycerol tube in a -20°C refrigerator was inoculated into the LB liquid medium in (1), and cultured at 30°C, 150 rpm for 12-16 h on a shaker to obtain a secondary liquid bacterial seed, OD 600 was 1.2-1.4;

[0044] (7) The heavy metal stock solutions in (2), (3), (4), and (5) were filtered through a 0.22 μm filter membrane, and LB liquid medium containing Cu 2+ , Ni + , Pb 2+ , or Cd 2+ at final concentrations of 50, 100, 300, 500, and 700 mg / L, respectively, was prepared; 2+ , Ni + , Pb 2+ , or Cd 2+ at final concentrations of 50, 100, 300, 500, and 700 mg / L, respectively, was prepared;

[0045] (8) The activated liquid bacterial seed in (6) was inoculated into the LB medium prepared in (7) at an inoculation amount of 10% (V / V) and cultured at 30°C, 150 rpm for 48 h;

[0046] The minimum inhibitory concentrations of MSC14 strain to different heavy metals were roughly determined by liquid culture method.

[0047] 1.2 Gradient plate method for determining the minimum inhibitory concentrations of MSC14 strain to different heavy metals

[0048] (1) LB solid medium was prepared with the following formula: 10 g / L tryptone, 10 g / L NaCl, 5 g / L yeast extract, and 20 g / L agar powder, and sterilized at 121°C for 20 min;

[0049] (2) 1.476 g of anhydrous copper nitrate was weighed on a balance and dissolved in an appropriate amount of ultrapure water, and then made up to 50 mL to obtain a copper nitrate stock solution;

[0050] (3) 2.239 g of nickel sulfate hexahydrate was weighed on a balance and dissolved in an appropriate amount of ultrapure water, and then made up to 50 mL to obtain a nickel sulfate stock solution;

[0051] (4) 0.799 g of anhydrous lead nitrate was weighed on a balance and dissolved in an appropriate amount of ultrapure water, and then made up to 50 mL to obtain a lead nitrate stock solution;

[0052] (5) 1.052 g of anhydrous cadmium nitrate was weighed on a balance and dissolved in an appropriate amount of ultrapure water, and then made up to 50 mL to obtain a cadmium nitrate stock solution;

[0053] (6) The bacterial seed liquid stored in a glycerol tube in a -20°C refrigerator was inoculated into the LB liquid medium in (1), and cultured at 30°C, 150 rpm for 12-16 h on a shaker to obtain a secondary liquid bacterial seed, OD 600 was 1.2-1.4;

[0054] (7) Take the heavy metal stock solution in (2) (3) (4) (5) respectively, filter through 0.22 μm filter membrane, prepare LB solid medium with final concentration of 200, 300, 400 mg / L Cu 2+ , LB solid medium with 100, 200, 300 mg / L Ni + , LB solid medium with 600, 700, 800 mg / L Pb 2+ , LB solid medium with 50, 100, 200 mg / L Cd 2+ , LB solid medium with 50, 100, 200 mg / L Cd 2+ , each take 15 ml while hot pour flat, save for later use;

[0055] (8) The activated liquid strain in (6) is inoculated into the prepared LB solid medium in (7) with 0.1 mL inoculation amount, and incubated in a 37℃ constant temperature incubator for 48 h Figure 1 ).

[0056] Table 1 Minimum inhibitory concentration of different heavy metals

[0057]

[0058] Example 2: Removal of low concentration of benzo[a]pyrene by MSC14 strain in water environment under heavy metal stress

[0059] 2.1 Removal of low concentration of benzo[a]pyrene by MSC14 strain in water environment under copper stress

[0060] (1) Prepare LB liquid medium, formula: 10 g / L tryptone, 10 g / L NaCl, 5 g / L yeast extract, 121℃ sterilization for 20 min;

[0061] (2) Weigh 1.476 g of anhydrous copper nitrate on a balance, dissolve in appropriate ultrapure water, and then make up to 50 mL to obtain a copper nitrate stock solution;

[0062] (3) Prepare inorganic salt medium containing benzo[a]pyrene and copper, formula: 1.00 g / L ammonium sulfate, 0.20 g / L potassium dihydrogen phosphate, 2.02 g / L sodium hydrogen phosphate, 0.20 g / L magnesium sulfate, 0.005 g / L ferric chloride, 0.001 g / L ammonium molybdate, 0.10 g / L calcium chloride, pH = 7.2 ± 0.1, 121℃ sterilization for 20 min, add benzo[a]pyrene filtered by 0.22 μm filter membrane to make the concentration of benzo[a]pyrene in the medium 20 mg / L, add the copper nitrate stock solution filtered by 0.22 μm filter membrane to make the Cu 2+ concentration in the medium 1, 10, 100, 300, 600, 900 mg / L, respectively;

[0063] (4) The bacterial seed liquid stored in a glycerol tube at -20°C was inoculated into the LB liquid culture medium in (1), and cultured on a shaking table at 30°C and 150 rpm for 12-16 hours to obtain the secondary liquid culture. The OD 600 1.2~1.4;

[0064] (5) The activated seed solution in (4) was inoculated into the inorganic salt medium containing benzo[a]pyrene and copper prepared in (3) at a 10% (V / V) inoculum and cultured at 30°C and 150 rpm for 5 days;

[0065] (6) HPLC detection of benzo[a]pyrene removal efficiency: The 0-day and 5-day samples were fully extracted twice with equal volumes of dichloromethane, and the volume was fixed to 25 mL. 1 mL was filtered through a 0.22 μm organic filter membrane and placed in an injection vial. A 4.5 mm × 250 mm Eclipse PAH column was used, the column temperature was 30°C, the mobile phase was 90%:10% acetonitrile:ultrapure water, the flow rate was 1 mL / min, and the UV detector was used for detection at a wavelength of 290 nm.

[0066] Table 2 Degradation rate of benzo[a]pyrene by strains under different concentrations of copper stress

[0067]

[0068]

[0069] From Table 2 and Figure 2 It can be seen that copper at a concentration of 1 mg / L can promote the degradation of benzo[a]pyrene by the MSC14 strain; copper at concentrations below 300 mg / L has little effect on benzo[a]pyrene degradation by the MSC14 strain; and copper at concentrations above 600 mg / L significantly inhibits benzo[a]pyrene degradation by the MSC14 strain. This suggests that the MSC14 strain can be used for the remediation of sites contaminated by heavy metals and polycyclic aromatic hydrocarbons.

[0070] 2.2 Removal of low-concentration benzo[a]pyrene by MSC14 in aquatic environments under nickel, lead, and cadmium stress

[0071] (1) Prepare LB liquid medium with the following formula: 10 g / L tryptone, 10 g / L NaCl, 5 g / L yeast extract, and sterilize at 121°C for 20 min.

[0072] (2) Weigh 2.239 g of nickel sulfate hexahydrate on a balance and dissolve it in an appropriate amount of ultrapure water. After it is fully dissolved, dilute to 50 mL to obtain a nickel sulfate stock solution.

[0073] (3) Weigh 0.799 g of anhydrous lead nitrate on a balance and dissolve it in an appropriate amount of ultrapure water. After sufficient dissolution, dilute to 50 mL to obtain a lead nitrate stock solution.

[0074] (4) Balance to take anhydrous cadmium nitrate 1.052 g dissolved in the appropriate amount of ultrapure water, after fully dissolved, constant volume to 50 mL, to obtain a cadmium nitrate stock solution;

[0075] (5) Configuration containing benzene [a] pyrene and nickel or lead or cadmium inorganic salt medium, formula: 1.00 g / L ammonium sulfate, 0.20 g / L potassium dihydrogen phosphate, 2.02 g / L sodium phosphate dibasic, 0.20 g / L magnesium sulfate, 0.005 g / L ferric chloride, 0.001 g / L ammonium molybdate, 0.10 g / L calcium chloride, pH = 7.2 ± 0.1, 121 ℃ sterilization 20 min, add 0.22 μm filter membrane filtered bacteria-free benzene [a] pyrene, so that the concentration of benzene [a] pyrene in the medium is 20 mg / L. Respectively, the heavy metal stock solution in (2) (3) (4) is filtered by 0.22 μm filter membrane, and then added, so that the concentration of Ni + in the medium is 1, 50, 100, 200 mg / L, Pb 2+ in the medium is 1, 100, 300, 700 mg / L, Cd 2+ in the medium is 1, 10, 50, 100 mg / L;

[0076] (6) The bacterial seed liquid stored in the glycerol tube in the -20 ℃ refrigerator is inoculated into the LB liquid medium in (1), and cultured at 30 ℃, 150 rpm on the shaking table for 12-16 h to obtain a secondary liquid bacterial seed, OD 600 is 1.2-1.4;

[0077] (7) The activated seed liquid in (6) is inoculated into the benzene [a] pyrene and nickel or lead or cadmium containing inorganic salt medium prepared in (5) at a 10% (V / V) inoculation amount, and cultured at 30 ℃, 150 rpm for 5 days;

[0078] (8) High performance liquid chromatography method for detecting the removal efficiency of benzene [a] pyrene: the 0 day and 5 day samples are extracted twice with an equal volume of dichloromethane, constant volume to 25 mL, 1 mL is filtered by 0.22 μm organic filter membrane, and then placed in the sample vial, 4.5 mm x 250 mm Eclipse PAH chromatographic column, column temperature is 30 ℃, mobile phase is 90%:10% acetonitrile: ultrapure water, flow rate is 1 mL / min, ultraviolet detector detects at 290 nm wavelength.

[0079] Table 3 Degradation rate of MSC14 strain to benzene [a] pyrene under different concentrations of heavy metal stress

[0080]

[0081] From Table 3 and Figure 3It can be seen that the method of the present application is also applicable to pollutants containing heavy metals nickel, lead and cadmium; under the condition of low concentration of heavy metals, the promotion of heavy metal lead to the degradation of polycyclic aromatic hydrocarbons by Pseudomonas stutzeri MSC14 is more optimal.

[0082] Example 3: Metal removal capacity of MSC14 strain under heavy metal and benzo[a]pyrene stress

[0083] (1) LB liquid medium was configured, and the formula was: 10 g / L tryptone, 10 g / L NaCl, 5 g / L yeast extract, sterilized at 121 ℃ for 20 min;

[0084] (2) 2.239 g of nickel sulfate hexahydrate was weighed on a balance and dissolved in an appropriate amount of ultrapure water, and after complete dissolution, the volume was made up to 50 mL to obtain a nickel sulfate stock solution;

[0085] (3) 0.799 g of anhydrous lead nitrate was weighed on a balance and dissolved in an appropriate amount of ultrapure water, and after complete dissolution, the volume was made up to 50 mL to obtain a lead nitrate stock solution;

[0086] (4) 1.052 g of anhydrous cadmium nitrate was weighed on a balance and dissolved in an appropriate amount of ultrapure water, and after complete dissolution, the volume was made up to 50 mL to obtain a cadmium nitrate stock solution;

[0087] (5) Inorganic salt medium containing benzo[a]pyrene and nickel or lead or cadmium was configured, and the formula was: 1.00 g / L ammonium sulfate, 0.20 g / L potassium dihydrogen phosphate, 2.02 g / L sodium phosphate dibasic, 0.20 g / L magnesium sulfate, 0.005 g / L ferric chloride, 0.001 g / L ammonium molybdate, 0.10 g / L calcium chloride, pH = 7.2 ± 0.1, sterilized at 121 ℃ for 20 min, and benzo[a]pyrene filtered by a 0.22 μm filter was added to make the concentration of benzo[a]pyrene in the medium 20 mg / L. The heavy metal stock solutions in (2), (3) and (4) were filtered by a 0.22 μm filter to remove bacteria, and then added to make the concentration of Ni + 50, 100, 200 mg / L, Pb 2+ 100, 300, 700 mg / L, Cd 2+ 10, 50, 100 mg / L, respectively;

[0088] (6) The bacterial seed liquid stored in a glycerol tube in a -20 ℃ refrigerator was inoculated into the LB liquid medium in (1), and cultured at 30 ℃, 150 rpm on a shaking table for 12-16 h to obtain a secondary liquid bacterial seed, and the OD 600 was 1.2-1.4;

[0089] (7) the activated seed liquid in (6) is inoculated into the prepared inorganic salt culture medium containing benzene[a]pyrene and nickel or lead or cadmium in (5) at an inoculation amount of 10% (V / V) and cultured at 30°C and 150 rpm for 5 days;

[0090] (8) Atomic absorption spectrophotometer detects the residual heavy metal concentration in the culture medium: 10mL of 0-day and 5-day samples are taken, the supernatant is taken after centrifugation, diluted to an appropriate concentration with 2% dilute nitric acid, and then detected by atomic absorption spectrophotometer (acetylene-air method).

[0091] Table 4 Removal capacity of MSC14 strain to metals under heavy metal and benzene[a]pyrene stress

[0092]

[0093]

[0094] It can be seen from Table 4 and Table 5 that the method of the present application can remove heavy metals while removing polycyclic aromatic hydrocarbons. Figure 4

[0095] Example 4: Removal of low-concentration polycyclic aromatic hydrocarbons by MSC14 strain in water environment under copper stress

[0096] (1) LB liquid medium is configured, and the formula is: 10 g / L of tryptone, 10 g / L of NaCl, and 5 g / L of yeast extract, sterilized at 121°C for 20 min;

[0097] (2) 1.476 g of anhydrous copper nitrate is weighed on a balance and dissolved in an appropriate amount of ultrapure water, and then diluted to 50 mL after complete dissolution to obtain a copper nitrate stock solution;

[0098] (3) Inorganic salt culture medium is configured, and the formula is: 1.00 g / L of ammonium sulfate, 0.20 g / L of potassium dihydrogen phosphate, 2.02 g / L of sodium phosphate dibasic, 0.20 g / L of magnesium sulfate, 0.005 g / L of ferric chloride, 0.001 g / L of ammonium molybdate, and 0.10 g / L of calcium chloride, pH = 7.2 ± 0.1, sterilized at 121°C for 20 min, and then 0.22 μm filter membrane is added to filter out the phenanthrene, fluorene, and fluoranthene, so that the concentrations of phenanthrene, fluorene, and fluoranthene in the culture medium are 20 mg / L, and the copper nitrate stock solution is added to filter out the bacteria through a 0.22 μm filter membrane, so that the Cu 2+ concentration in the culture medium is 100 mg / L;

[0099] (4) The bacterial seed liquid stored in a glycerol tube in a -20°C refrigerator is inoculated into the LB liquid medium in (1), and cultured at 30°C and 150 rpm for 12-16 h on a shaking table to obtain a secondary liquid bacterial seed, and the OD 600 is 1.2-1.4;

[0100] ​(5) The activated seed liquid in (4) is inoculated into the prepared inorganic salt medium in (3) at a 10% (V / V) inoculation amount and cultured at 30°C and 150 rpm for 5 days;

[0101] (6) High-performance liquid chromatography is used to detect the removal efficiency of phenanthrene, fluorene and fluoranthene: 0-day and 5-day samples are extracted twice with an equal volume of dichloromethane, diluted to 25 mL, 1 mL of which is filtered through a 0.22 μm organic filter membrane and placed in a sample vial, an Eclipse PAH chromatographic column with a size of 4.5 mm x 250 mm is used, the column temperature is 30°C, the mobile phase is 90%:10% acetonitrile:ultra-pure water, the flow rate is 1 mL / min, and a UV detector is used to detect at a wavelength of 290 nm.

[0102] Table 5: Polycyclic aromatic hydrocarbon degradation rate of MSC14 strain under different concentrations of copper stress

[0103]

[0104] It can be seen from Table 5 and Figure 5 that the method of the present application has universality for polycyclic aromatic hydrocarbons.

[0105] The above examples are preferred embodiments of the present application, but the embodiments of the present application are not limited by the above examples, and any changes, modifications, substitutions, combinations, simplifications made without departing from the spirit and principles of the present application are equivalent replacement methods and are included in the protection scope of the present application.

Claims

1. A method for simultaneous removal of polycyclic aromatic hydrocarbons and heavy metals, characterized by, The method comprises the following steps: Inoculating the contaminant containing heavy metals and polycyclic aromatic hydrocarbons with Pseudomonas dispersa MSC14, and removing the polycyclic aromatic hydrocarbons and heavy metals after culture; the preservation number of the Pseudomonas dispersa MSC14 is GDMCC No: 62680; the polycyclic aromatic hydrocarbons comprise at least one of benzo[a]pyrene, pyrene, fluoranthene, phenanthrene, fluorene and anthracene; and the heavy metals comprise at least one of copper, nickel, lead and cadmium.

2. The method of simultaneously removing polycyclic aromatic hydrocarbons and heavy metals of claim 1, wherein, The contaminant is a water body or soil.

3. The method of simultaneously removing polycyclic aromatic hydrocarbons and heavy metals of claim 1, wherein, The contaminant is a water body, and the concentration of the heavy metals is (0, 300] mg / L.

4. The method of simultaneously removing polycyclic aromatic hydrocarbons and heavy metals of claim 3, wherein, The contaminant is a water body, and the concentration of the heavy metals is (0, 1] mg / L.

5. The method of simultaneously removing polycyclic aromatic hydrocarbons and heavy metals of claim 1, wherein, The contaminant is a water body, and the concentration of the polycyclic aromatic hydrocarbons is (0, 50] mg / L.

6. The method of simultaneously removing polycyclic aromatic hydrocarbons and heavy metals of claim 5, wherein, The contaminant is a water body, and the concentration of the polycyclic aromatic hydrocarbons is (0, 20] mg / L.

7. Use of the method for simultaneously removing polycyclic aromatic hydrocarbons and heavy metals according to any one of claims 1-6 in the remediation of heavy metal and / or polycyclic aromatic hydrocarbon contaminated soil and wastewater.

Images