A method for land-based factory cultivation of bangia pterocaula seaweed

Abstract

The application discloses a land-based factory cultivation method of red seaweed of the genus Devalera, and belongs to the technical field of seaweed cultivation. The method comprises the following steps: preparation and construction of a cultivation system; preparation of a seed fixing net; inoculation and planting of the red seaweed of the genus Devalera; red seaweed seedling supplement; daily management and environmental regulation; harvesting of the red seaweed of the genus Devalera; and continued cultivation after harvesting. The red seaweed seedlings of the genus Devalera are planted on the seed fixing net in the cultivation pond, so that the stability of the red seaweed seedlings is enhanced; the red seaweed seedlings left after harvesting can be continuously cultivated, so that the cultivation times are increased; the cultivation water is circulated to cultivate the red seaweed seedlings, so that the stability of the whole cultivation ecological environment is maintained; and the cultivation water can be directly used for animal cultivation after being output. The circulation cultivation method increases the stability of the cultivation water body, reduces the breeding of exogenous microalgae and epiphytic weeds, improves the yield and quality of the cultivated varieties, and is favorable for the popularization of the large-scale cultivation of the seaweed.

Description

Technical Field

[0001] This invention relates to the field of seaweed aquaculture technology, and in particular to a land-based industrialized aquaculture method for seaweed of the genus *Pterocarya*. Background Technology

[0002] Large seaweeds have significant economic and ecological value. However, the development of large-scale land-based aquaculture of large seaweeds is limited by many factors. Currently, land-based factory farming of seaweeds of the genus *Amanita* has not yet been achieved. Summary of the Invention

[0003] The main objective of this invention is to provide a land-based industrialized cultivation method for *Amanita* seaweed, aiming to solve the technical problems of large-scale cultivation of *Amanita* seaweed.

[0004] To achieve the above objectives, the present invention provides a land-based industrialized cultivation method for *Rhododendron* seaweed, based on the following cultivation system apparatus:

[0005] The aquaculture system includes an aquaculture pond, a sedimentation tank, a pump, a disinfection and sterilization device, and a filtration device. The disinfection and sterilization device is used to disinfect and sterilize the water in the sedimentation tank. The pump is placed in the sedimentation tank, and its outlet is connected to the inlet of the filtration device. The outlet of the filtration device is connected to the inlet of the aquaculture pond. The outlet of the aquaculture pond is connected to the first inlet of the sedimentation tank. The second inlet of the sedimentation tank is used to input external seawater, and the outlet of the sedimentation tank is used to output aquaculture water, which can be used for animal husbandry. A seedling net is located in the aquaculture pond.

[0006] Based on the above apparatus, the land-based industrialized cultivation method for *Rhododendron* seaweed includes the following steps:

[0007] S10. Provide seedlings of the genus *Pterocarya* and inject sand-filtered seawater into the aquaculture system;

[0008] S20. Soak the Red-feathered seaweed seedlings in fresh water and inoculate them onto the seedling net of the aquaculture pond in the aquaculture system to obtain the first inoculated Red-feathered seaweed seedlings.

[0009] S30. On the second day after inoculation, the uninoculated red pea seedlings were collected and replanted in the uninoculated seedling net area to obtain inoculated red pea seaweed seedlings.

[0010] S40. Cultivate the inoculated red pea seaweed seedlings, harvest the red pea seaweed, and obtain red pea seaweed seedlings and aquaculture water;

[0011] S50. The aquaculture water is treated and then used to cultivate red pheasant seaweed seedlings. The red pheasant seaweed is harvested to obtain red pheasant seaweed seedlings and aquaculture water.

[0012] S60, Repeat step S50.

[0013] Optionally, the disinfection and sterilization device includes an ultraviolet lamp; and / or,

[0014] The filtration device includes a microfilter.

[0015] Optionally, in step S10, the seawater in the aquaculture system includes nutrients.

[0016] Optionally, the nutrients include nitrates, phosphates, and urea.

[0017] Optionally, in step S10, the seawater is in a dynamic process within the aquaculture cycle system;

[0018] The dynamic process includes the automatic discharge of some seawater from the aquaculture pond into a water storage and sedimentation tank, where it is settled, disinfected, microfiltered, and then pumped back into the aquaculture pond.

[0019] Optionally, in step S20, the soaking time of the red algae is 10-20 seconds.

[0020] Optionally, in steps S20 and S30: the planting density of the *Pterocarya* seaweed is 1~1.5 kg / m². 2 ; and / or,

[0021] The coverage of *Leptochloa* seaweed on the seedling bed is >50%.

[0022] Optionally, in step S40:

[0023] The light intensity for the aquaculture was 90~150 µmol×m. -2 ×s -1 ; and / or,

[0024] The culture temperature is 25~30℃; and / or,

[0025] The salinity of the cultured organisms is 26-32.

[0026] Optionally, in step S40: the red thorn seaweed is harvested after 28-45 days of cultivation;

[0027] When harvesting seaweed of the genus *Pterocarya*, the harvest length should be 4-6 cm.

[0028] Optionally, in step S50, the treatment of the aquaculture water includes the following steps:

[0029] S510. Part of the aquaculture water is discharged for animal breeding, and fresh seawater filtered by sand is added to the remaining aquaculture water to obtain mixed seawater.

[0030] S520. The mixed seawater is precipitated, disinfected, and microfiltered before being introduced into the aquaculture pond as circulating aquaculture water for seaweed of the genus *Pterocarya*.

[0031] This invention proposes a land-based industrialized cultivation method for *Rhododendron* seaweed. The method involves pre-soaking *Rhododendron* seedlings in freshwater and using sand filtration of seawater to reduce the amount of other algae in the cultivation pond. The seedlings are then planted on seedling nets within the cultivation pond, enhancing their stability. Seedlings are harvested and replanted the day after planting, improving seedling utilization. The remaining seedlings after harvest can be used to cultivate the next crop, increasing the number of cultivation cycles and reducing the costs of planting and seedling acquisition. The cultivation water is circulated, maintaining the stability of the entire cultivation ecosystem. The water used for cultivation can be directly used for animal husbandry, further increasing water utilization. This cyclical cultivation method increases the stability of the cultivation water body, reduces the growth of exogenous microalgae and epiphytic algae, and improves the yield and quality of the cultivated species. Attached Figure Description

[0032] Figure 1 This is a diagram of the scaffold-type seedling cultivation device for red-feathered cabbage in Embodiment 1 of the present invention;

[0033] Figure 2 Images of the red-feathered vegetable recirculating aquaculture system in Embodiment 1 of the present invention;

[0034] Figure 3 This is a picture of the red pea flower cultivation pond before inoculation in Embodiment 1 of the present invention;

[0035] Figure 4 This is a diagram of the experimental propagation of red tamarind in cyclic cultivation in Embodiment 1 of the present invention.

[0036] Explanation of icon numbers:

[0037]

[0038] The realization of the objective, functional features and advantages of the present invention will be further explained in conjunction with the embodiments and with reference to the accompanying drawings. Detailed Implementation

[0039] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below. Where specific conditions are not specified in the embodiments, conventional conditions or conditions recommended by the manufacturer shall apply. Where the manufacturers of reagents or instruments are not specified, they are all conventional products that can be purchased commercially. Furthermore, the meaning of "and / or" throughout the text includes three parallel solutions; for example, "A and / or B" includes solution A, or solution B, or a solution where both A and B are satisfied simultaneously. In addition, the technical solutions of the various embodiments can be combined with each other, but this must be based on the ability of those skilled in the art to implement them. When the combination of technical solutions is contradictory or cannot be implemented, it should be considered that such a combination of technical solutions does not exist and is not within the scope of protection claimed by the present invention. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0040] Large seaweeds have significant economic and ecological value. However, the development of large-scale land-based aquaculture of large seaweeds is limited by many factors. Currently, land-based factory farming of seaweeds of the genus *Amanita* has not yet been achieved.

[0041] genus *Callicarpa* ( Solieria Seaweed is a type of economic seaweed with a wide range of applications. In China, the main variety is *Pterocarya stenoptera* (Pacific red seaweed). Solieria pacifica ) and delicate red tamarind ( Solieria tenuis Two species of *Pterocarya* exhibit numerous advantages in terrestrial environments, including rapid growth, high temperature tolerance, and strong pollution resistance, making them suitable for terrestrial industrial aquaculture and possessing significant development value. They can be consumed directly and used medicinally, possessing high nutritional and health benefits, and can be developed into special prepared dishes, functional beverage additives, etc.; they can be used as a main raw material for extracting carrageenan; they can be applied as high-quality feed for farmed organisms; furthermore, they can effectively absorb nitrogen and phosphorus from eutrophic water bodies, playing a role in water purification, and can be used as a tool species for green integrated aquaculture and aquaculture wastewater treatment. These advantages indicate that *Pterocarya* seaweeds are a high-quality species suitable for large-scale industrial aquaculture.

[0042] Currently, the development and utilization of *Rhododendron* seaweed mainly relies on wild resources, lacking an effective cultivation technology and failing to achieve large-scale farming. Therefore, it is necessary to establish a land-based industrialized cultivation method for *Rhododendron* seaweed to ensure the diversification of suitable species for land-based industrialized cultivation of large seaweeds.

[0043] In view of this, the present invention proposes a land-based industrialized cultivation method for *Azolla* seaweed, which is based on the following cultivation system device:

[0044] The aquaculture system includes an aquaculture pond 200, a sedimentation tank 300, a pump 400, a disinfection and sterilization device 500, and a filtration device 600. The disinfection and sterilization device 500 is used to disinfect and sterilize the water in the sedimentation tank 300. The pump 400 is placed in the sedimentation tank 300. The outlet of the pump 400 is connected to the inlet of the filtration device 600 via a pipe 700. The outlet of the filtration device 600 is connected to the inlet of the aquaculture pond 200 via a pipe 700. The outlet of the aquaculture pond 200 is connected to the first inlet of the sedimentation tank 300 via a pipe 700. The second inlet of the sedimentation tank 300 receives external seawater via a pipe 700. The outlet of the sedimentation tank 300 outputs aquaculture water via a pipe 700. The output aquaculture water can be used for animal husbandry. A seedling net 2 is located on the seaweed culture bed 100 in the aquaculture pond 200.

[0045] Based on the above apparatus, the land-based industrialized cultivation method for *Rhododendron* seaweed includes the following steps:

[0046] S10. Provide seedlings of the genus *Pterocarya* and inject sand-filtered seawater into the aquaculture system;

[0047] S20. Soak the Red-feathered seaweed seedlings in fresh water and inoculate them onto the seedling net 2 of the aquaculture pond 200 in the aquaculture system to obtain the first inoculated Red-feathered seaweed seedlings.

[0048] S30. On the second day after inoculation, the uninoculated red pea seedlings were collected and replanted in the uninoculated seedling net area 2 to obtain inoculated red pea seaweed seedlings.

[0049] S40. Cultivate the inoculated red pea seaweed seedlings, harvest the red pea seaweed, and obtain red pea seaweed seedlings and aquaculture water;

[0050] S50. The aquaculture water is treated and then used to cultivate red pheasant seaweed seedlings. The red pheasant seaweed is harvested to obtain red pheasant seaweed seedlings and aquaculture water.

[0051] S60, Repeat step S50.

[0052] Before inoculating and planting seaweed seedlings of the genus *Pterocarya*, disinfect the inoculation materials, aquaculture equipment, and aquaculture ponds with a high concentration of bleaching powder or chlorine tablets.

[0053] The aquaculture system injects sand-filtered seawater. Sand filtration is performed at least three times to remove large particles of debris and unwanted algae from the seawater as much as possible. Before injecting fresh seawater into the aquaculture tank, the seawater is sterilized to reduce the possibility of plankton causing disease to the *Pterocarya* genus algae.

[0054] The selected seaweed seedlings of the genus *Pterocarya* are those with vibrant color, intact rhizomes, and no signs of rot. The *Pterocarya* genus includes either *Pterocarya slendera* or *Pterocarya pacifica*.

[0055] In this invention, the seedlings of *Pterocarya* species are pre-soaked in fresh water and seawater is filtered through sand to reduce the amount of other algae in the aquaculture pond. The seedlings are then planted on seedling nets in the aquaculture pond, enhancing their stability. Seedlings are collected and replanted on the second day after planting, improving seedling utilization. The remaining seedlings after harvest can be used to cultivate the next crop, increasing the number of cultivation cycles and reducing the cost of planting and seedling acquisition. The aquaculture water is circulated to maintain the stability of the entire aquaculture ecosystem. The water can be directly used for animal husbandry after being exported, further increasing water utilization. This cyclical aquaculture method increases the stability of the aquaculture water, reduces the growth of exogenous microalgae and epiphytic algae, and improves the yield and quality of the cultured species.

[0056] The cultivation method of this invention can be used for the cultivation of *Rhododendron* seaweed. The method is simple to operate, and multiple harvests and continuous cultivation increase yields, save on manual inoculation costs, and the circulating system maintains the stability of the entire cultivation ecosystem, effectively inhibiting the growth of exogenous microalgae and epiphytic algae, thus optimizing the quality of the cultivated species. Furthermore, the water used for cultivation, after being purified by *Rhododendron* seaweed, can be directly supplied to animal husbandry, increasing seawater utilization and animal husbandry efficiency, which is conducive to the large-scale cultivation and promotion of this seaweed.

[0057] In some embodiments of the present invention, the disinfection and sterilization device 500 includes an ultraviolet lamp; the filtration device 600 includes a microfilter. In water treatment, ultraviolet disinfection is a chemical-free method used to remove harmful microorganisms and plankton from water. Ultraviolet irradiation is harmful to single-celled or multi-celled microorganisms such as phytoplankton, causing them to die in large numbers. Therefore, in practical applications, the ultraviolet lamp is placed in the sedimentation tank 300 to prevent *Plasmodium* algae from being directly exposed to ultraviolet radiation. Simultaneously, ultraviolet irradiation can reduce the reproduction of phytoplankton spores in the water, minimizing their contamination of *Plasmodium* algae and preventing any impact on its growth rate and appearance.

[0058] The filtration device 600 includes a microfilter. Microfiltration is a technique for liquid separation using a filter medium with micron-sized pores. In microfiltration, the solution passes through a porous membrane, the pore size of which is typically between 0.1 and 10 micrometers. This membrane effectively traps particulate matter, bacteria, microorganisms, and certain macromolecules in the solution, while allowing water and small molecule solutes to pass through.

[0059] By combining ultraviolet light disinfection with microfiltration, the survival rate of miscellaneous algal spores and the possibility of miscellaneous algae growth in seawater are reduced, thereby ensuring water cleanliness and preventing contamination of red algae by miscellaneous algae as much as possible.

[0060] In any embodiment of the present invention, in step S10, the seawater in the aquaculture system includes nutrients; the nutrients include nitrates, phosphates, and urea. During the recirculating aquaculture process, *Pterocarya* seedlings absorb nutrients from the seawater, leading to a decrease in the nutrient content of the seawater. Therefore, it is necessary to replenish nutrients in the aquaculture pond 200 to ensure that the subsequent aquaculture of *Pterocarya* is not limited by nutrients. By mixing and adding nitrates, phosphates, and urea, sufficient nutrition can be ensured for the *Pterocarya* seedlings, resulting in faster growth and a shorter aquaculture time.

[0061] In any embodiment of the present invention, in step S10, the seawater is in a dynamic process within the aquaculture circulation system. This dynamic process includes the automatic discharge of a portion of the seawater from the aquaculture pond 200 into a sedimentation tank 300 for sedimentation, disinfection, microfiltration, and then pumping it back into the aquaculture pond 200. Initially, a certain volume of sand-filtered seawater is injected into the sedimentation tank 300, followed by sedimentation, sterilization, and disinfection. Then, it is pumped into the aquaculture pond 200 through microfiltration. Once the aquaculture pond 200 is full, the seawater is automatically discharged into the sedimentation tank 300. Throughout the aquaculture process, the volume of seawater in the aquaculture system remains constant, and the seawater is in a dynamic flow process. This dynamic seawater circulation increases the fluidity of the seawater, and the resulting water flow promotes the growth of algae while simultaneously improving seawater utilization.

[0062] When the sealing of the circulation pipes in the aquaculture tank deteriorates, such as a rupture in the inlet / outlet pipe leading to seawater leakage, additional sand-filtered fresh seawater should be added to the entire recirculating aquaculture system. Normally, the original amount of seawater in the aquaculture system should be maintained without increase or decrease.

[0063] In any embodiment of the present invention, in step S20, the soaking time of the *Amanita* seaweed is 10-20 seconds. This soaking time range helps to protect the *Amanita* seaweed seedlings from freshwater damage and minimizes the presence of other algae on the seedling surface.

[0064] In any embodiment of the present invention, in steps S20 and S30, the planting density of the *Amanita* seaweed is 1~1.5 kg / m². 2 The inoculation and planting density should be less than 1 kg / m². 2 At times, if the algal strain is too sparse, the cultivation cycle is prolonged, it is easily contaminated by other algae, and it consumes cultivation space; if the inoculation and planting density is higher than 1.5 kg / m², the cultivation space is also reduced. 2 At times, the algae species are too dense, overlapping and squeezing each other, which wastes the algae species.

[0065] In any embodiment of the present invention, in steps S20 and S30, the coverage of *Pterocarya* seaweed on the seedling net is >50%. During inoculation and planting, the inoculation amount should be carefully controlled, ensuring the seedlings are spread evenly across the seedling net 2 and achieve a coverage of over 50%. The seedling coverage of the seedling net 2 is crucial; appropriate seedling coverage affects the later growth of the algae, shortens the harvest time, and reduces the degree of contamination from other algae.

[0066] The day after inoculation and transplanting, any scattered or floating *Pterocarya* seedlings were collected, and areas with low coverage were replanted to maintain seedling density and coverage. Low coverage refers to areas where seedlings were scattered on the seedling net 2, and areas where the inoculation density did not reach 1-1.5 kg / m². 2 The area.

[0067] In any embodiment of the present invention, in step S40: the light intensity for the aquaculture is 90~150 µmol×m -2 ×s -1 Light intensity below 90 µmol × m -2 ×s -1 The thallus of the genus *Pterocarya* grows slowly and requires light intensity above 150 µmol × m². -2 ×s -1 It causes damage to the thallus of the genus *Amanita* and causes it to turn yellow.

[0068] In any embodiment of the present invention, in step S20, the cultivation temperature is 25~30℃. Excessively high or low cultivation temperatures will cause seaweed to rot and be lost, especially temperatures below 18℃ for extended periods, which can easily lead to large-scale algal death.

[0069] In any embodiment of the present invention, in step S20, the aquaculture salinity is 26-32. Both excessively high and low salinity can lead to algal rot, with prolonged salinity below 20 resulting in more severe rot. During periods of continuous rain, seawater replenishment is generally stopped until the weather stabilizes and the salinity recovers before resuming seawater replenishment.

[0070] In some embodiments of the present invention, in step S40: the *Pterocarya* seaweed is harvested after 28-45 days of cultivation; when harvesting the *Pterocarya* seaweed, the harvested length is 4-6 cm. That is, the *Pterocarya* seaweed is harvested when it has sprouted from seedlings and grown to a height of 4-6 cm. The purpose of harvesting the *Pterocarya* seaweed at this height is to maintain a relatively high growth rate. When the height of the *Pterocarya* seaweed exceeds 6 cm, its growth rate slows down. To increase the cultivation yield, a second round of cultivation is carried out after harvesting.

[0071] In addition, during the 28-45 days between inoculation and harvest of *Amanita* seaweed, it is necessary to continuously check for algal contamination, adhering to the principle of early detection and treatment. Diatoms and filamentous algae are the most serious contaminants; depending on the cultivation situation, the algae should be rinsed with fresh water for 5 minutes to remove diatoms. For some filamentous algae, fresh water treatment is ineffective, and manual treatment is required. Clean the contaminated areas in real time, remove rotten algae, and rinse the seaweed. For cultivation ponds with severe algal contamination, clean the pond and re-inoculate as soon as possible to avoid wasting cultivation time.

[0072] In some embodiments of the present invention, step S50, the treatment of the aquaculture water includes the following steps:

[0073] S510. Part of the aquaculture water is discharged for animal breeding, and fresh seawater filtered by sand is added to the remaining aquaculture water to obtain mixed seawater.

[0074] S520. The mixed seawater is precipitated, disinfected, and microfiltered before being introduced into the aquaculture pond 200 as circulating aquaculture water for seaweed of the genus *Pterocarya*.

[0075] Because red algae effectively absorb nitrogen and phosphorus from eutrophic waters during cultivation, thus purifying the water, the water used for cultivating red algae is of good quality and suitable for raising animals or aquatic products.

[0076] A pump body 400 is installed in the water storage sedimentation tank 300. The outlet of the pump body 400 is connected to the inlet of the filter device 600. The pump body 400 is used to continuously pump the seawater that has been sand filtered, settled and ultraviolet disinfected in the water storage sedimentation tank 300 into the filter device 600, and after microfiltration, it is reintroduced into the aquaculture tank 200.

[0077] A second inlet and an outlet are provided in the sedimentation tank 300. The second inlet is used to input externally filtered seawater; the outlet is used to output a portion of the aquaculture water, which can be used for animal or aquatic product farming. The externally filtered seawater mixes with the remaining aquaculture water in the aquaculture system to form mixed seawater. This mixed seawater, after sedimentation, disinfection, and microfiltration, enters the aquaculture tank 200 as recirculating aquaculture water for *Pterocarya* seaweed. The input volume of the externally filtered seawater equals the output volume of the aquaculture water. This process is repeated continuously, with externally filtered seawater constantly inputting and *Pterocarya* seaweed aquaculture water constantly outputting, increasing the uses of aquaculture water and forming a green integrated aquaculture system.

[0078] The technical solution of the present invention will be further described in detail below with reference to specific embodiments. It should be understood that the following embodiments are only used to explain the present invention and are not intended to limit the present invention.

[0079] Example 1

[0080] A method for land-based industrialized cultivation of seaweed of the genus *Pteris* includes the following steps:

[0081] Figure 1 A seaweed cultivation bed 100 includes a support frame 1 and a seedling net 2. The support frame 1 includes a frame 11 and four support pipes 12, which are connected to the bottom of the support frame 1. The frame 11 consists of four 1m long 32mm PVC pipes, which are connected by four three-dimensional four-way connectors to form a rectangular frame. The seedling net 2 consists of two 1×1m rigid plastic mesh sheets with a mesh diameter controlled at 1cm. One mesh sheet is used to lay seaweed seedlings, and the other mesh sheet covers the mesh sheet on which the seaweed seedlings are laid. Two mesh sheets are fixed to the frame 11 with 20cm long cable ties to achieve the effect of inoculating and planting seedlings; the support pipe 12 consists of four 30cm 32PVC pipes, the length of which can be adjusted according to the water level of the aquaculture pond. The support pipe 12 is connected to the frame 11 by a three-dimensional four-way hole; the extra hole in the three-dimensional four-way hole of the entire device can freely enter seawater and sink into the water, so that the support pipe 12 can be stably submerged at the bottom of the water body, thereby allowing the seaweed culture bed 100 to stand stably in the water body.

[0082] Figure 2 This refers to a land-based, factory-scale aquaculture system for seaweed of the genus *Amaranthus*. For example... Figure 2 As shown, the basic facilities of the constructed aquaculture system mainly include six 4×5×1.5m... 3 Aquaculture ponds 200m and 40m 3 The system includes a 300-meter sedimentation tank, a 400-meter 3000W water pump, a 600-meter microfiltration unit, a 500-meter ultraviolet (UV) disinfection device, and a 700-meter piping system. The aquaculture pond 200 is an indoor, factory-produced cement pond that can normally receive and discharge seawater and is exposed to natural light. The sedimentation tank 300 and UV lamp are used for sedimentation and disinfection of aquaculture wastewater. The drain pipe, water pump 400, and inlet pipe are used to pump out and inject circulating seawater into the aquaculture pond, respectively. The water supply pipe and microfiltration unit are used to replenish fresh seawater and filter the circulating seawater. The entire piping system 700 enables seawater circulation.

[0083] S10. First, disinfect the inoculation materials, aquaculture equipment, and aquaculture pond 200 with high-concentration bleaching powder or chlorine tablets. Then, treat fresh seawater with sand filtration, injecting the sand-filtered seawater into the sedimentation tank 300 for sedimentation. Simultaneously, turn on the ultraviolet disinfection device 500 for disinfection, and turn on the water pump 400 to pump the sand-filtered seawater from the sedimentation tank 300 into the microfiltration device 600 for filtration, and then pump it into the aquaculture pond 200. Once the seawater in the aquaculture pond 200 is full, it flows directly into the sedimentation tank 300, thus creating a circulating seawater system throughout the aquaculture process. Furthermore, due to the water purification effect of the Pacific Red-winged Seaweed, the circulating water from the Pacific Red-winged Seaweed aquaculture is directly supplied to the animals.

[0084] S20, 200 culture ponds before inoculation (see image) Figure 3 As shown. Select brightly colored, intact rootstocks and healthy, rot-free Pacific Redfin Sedge seedlings, and quickly soak them in fresh water for 20 seconds. After soaking, transplant the seedlings using cable ties into three 0.3×0.3m plots. 2 On the double-layer seedling net 2 of the seaweed culture bed 100, 100g of seedlings are inoculated onto each seaweed culture bed 100. After inoculating and planting Pacific red pheasant seedlings, the entire seaweed culture bed 100 is neatly placed into the same culture pond to obtain the first inoculated red pheasant seaweed seedlings.

[0085] On the second day after inoculation of S30 and Pacific red tassel seaweed, the scattered floating seaweed seeds were collected, and seedlings were added to areas with low coverage to maintain the seedling inoculation density and coverage, so as to obtain well inoculated red tassel seaweed seedlings.

[0086] S40. Adjust the light intensity of the Pacific red pea plant cultivation environment to 90 µmol × m -2 ×s -1 The growth temperature was adjusted to 25℃ and the growth salinity to 26, and Pacific red tassel grass was cultivated.

[0087] When the Pacific red tamarind protruded 4 cm above the surface of the seedling net, the protruding portion was manually trimmed, while retaining the algae seed between the two layers of netting. The Pacific red tamarind was harvested on the 28th day of cultivation, with a total weight of 1348 g. Specific harvesting details are shown in Table 1.

[0088] Table 1. Harvesting Status of Pacific Red Pea Shoots

[0089]

[0090] After harvesting Pacific red pea shoots ( Figure 4 The 200-cubic-meter aquaculture pond contains Pacific red pea seedlings and aquaculture water.

[0091] 3 x 0.3m 2 The initial weight of *Pterocarya stenoptera* in the seaweed culture beds was 110g, with a total weight of 330g. After 28 days, the *Pterocarya stenoptera* was harvested and weighed. The weights of the *Pterocarya stenoptera* harvested from the three seaweed culture beds were 445g, 478g, and 425g, respectively, with a total weight of 1348g. Its growth rate reached 135g / (D×m). 2 The weight gain rate was 3.08%.

[0092] S50. A portion of the aquaculture water is discharged for animal farming, and fresh seawater filtered by sand is added to the remaining aquaculture water to obtain mixed seawater. The mixed seawater is then precipitated, disinfected, and micro-filtered before being introduced into the aquaculture pond 200 as circulating aquaculture water for the genus *Pterocarya* to continue cultivating *Pterocarya pacifica*, resulting in *Pterocarya pacifica* seedlings and aquaculture water.

[0093] S60, Repeat step S50.

[0094] The above are merely preferred embodiments of the present invention and do not limit the patent scope of the present invention. Various modifications and variations can be made to the present invention by those skilled in the art. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the patent protection scope of the present invention.

Claims

1. A method for land-based industrialized cultivation of *Pterocarya* seaweed, characterized in that, Based on the following aquaculture system: The aquaculture system includes an aquaculture pond, a sedimentation tank, a pump, a disinfection and sterilization device, and a filtration device. The disinfection and sterilization device disinfects the water in the sedimentation tank. The pump is placed in the sedimentation tank, and its outlet is connected to the inlet of the filtration device. The outlet of the filtration device is connected to the inlet of the aquaculture pond. The outlet of the aquaculture pond is connected to the first inlet of the sedimentation tank. The second inlet of the sedimentation tank is used to input external seawater, and the outlet of the sedimentation tank is used to output aquaculture water, which can be used for animal husbandry. Several seaweed cultivation beds are provided. Each seaweed cultivation bed includes a support frame and a seedling net. The support frame includes a frame and four support pipes, which are connected to the bottom of the support frame. The frame consists of four PVC pipes, each connected by four three-dimensional four-way connectors to form a rectangular frame. The seedling net includes two hard plastic mesh sheets, one of which is used to lay seaweed seedlings, and the other mesh sheet covers the mesh sheet on which the seaweed seedlings are laid. The two mesh sheets are fixed to the frame with cable ties. The support pipes are connected to the frame by three-dimensional four-way connectors, and one of the additional holes in the three-dimensional four-way connectors of the seaweed cultivation bed allows seawater to flow freely. Based on the above apparatus, the land-based industrialized cultivation method for *Rhododendron* seaweed includes the following steps: S10. Provide seedlings of the genus *Pterocarya* and inject sand-filtered seawater into the aquaculture system; S20. Soak the *Pterocarya* seaweed seedlings in fresh water and inoculate them onto the seedling net of the aquaculture pond in the aquaculture system to obtain the first-time inoculated *Pterocarya* seaweed seedlings. The soaking time of the *Pterocarya* seaweed seedlings is 10-20 seconds. S30. On the second day after inoculation, the uninoculated red pea seedlings were collected and replanted in the uninoculated seedling net area to obtain inoculated red pea seaweed seedlings. S40. Cultivate the inoculated red pheasant seaweed seedlings, harvest the red pheasant seaweed, and obtain red pheasant seaweed and cultivation water. When harvesting the red pheasant seaweed, manually cut the part of the net that protrudes from the top, and retain the seaweed seedlings between the two layers of net. The harvesting length of the red pheasant seaweed is 4~6cm. S50. The aquaculture water is treated and then used to cultivate red pheasant seedlings. The red pheasant seaweed is harvested to obtain red pheasant seaweed and aquaculture water. S60, Repeat step S50; In steps S20 and S30: the planting density of the *Pterocarya* seaweed is 1~1.5 kg / m². 2 The coverage of *Pterocarya* seaweed on the seedling bed is >50%. In step S10, the seawater in the aquaculture system includes nutrients; In step S10, the seawater is in a dynamic process within the aquaculture cycle system; The dynamic process includes the automatic discharge of some seawater from the aquaculture pond into a water storage and sedimentation tank, followed by sedimentation, disinfection, microfiltration, and then pumping it back into the aquaculture pond. In step S40: The light intensity for the aquaculture was 90~150 μmmol×m. -2 ×s -1 ; The breeding temperature is 25~30℃; The salinity for aquaculture is 26-32. In step S40: The red pheasant seaweed is harvested after 28-45 days of cultivation.

2. The land-based industrialized cultivation method for *Amanita* seaweed as described in claim 1, characterized in that, The disinfection and sterilization device includes an ultraviolet lamp; and / or, The filtration device includes a microfilter.

3. The land-based industrialized cultivation method for *Amanita* seaweed as described in claim 1, characterized in that, The nutrients include nitrates, phosphates, and urea.

4. The land-based industrialized cultivation method for *Amanita* seaweed as described in claim 1, characterized in that, In step S50, the treatment of the aquaculture water includes the following steps: S510. Part of the aquaculture water is discharged for animal breeding, and fresh seawater filtered by sand is added to the remaining aquaculture water to obtain mixed seawater. S520. The mixed seawater is precipitated, disinfected, and microfiltered before being introduced into the aquaculture pond as circulating aquaculture water for seaweed of the genus *Pterocarya*.

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