A kind of nutritional supplement for pet kittens and preparation method thereof

Abstract

The invention discloses a nutritional supplement for pet kittens, comprising the following raw materials in weight proportions: 500-1000 parts of lactoferrin, 150-400 parts of fructooligosaccharides, 150-400 parts of manno-oligosaccharides, 150-400 parts of inulin, 125 parts of yam extract, 100 parts of vitamin C, 50 parts of freeze-dried bovine spleen polypeptides, and 20-53 parts of probiotics, and discloses its preparation method, which has a simple preparation process and is easy to promote. The invention is natural and safe, mainly for the immune system of pet cats in the weaning period and infancy that has not yet fully matured, cannot produce enough antibodies to resist diseases, and is prone to body inflammation problems specifically manifested as vomiting, diarrhea, psoriasis and other discomfort symptoms, improves the overall immunity of pet kittens and the hair state of kittens, and the product of the invention is applicable to animal groups, animal age groups and composition formulas with obvious characteristics.

Description

Technical Field

[0001] The invention belongs to the technical field of pet nutritional supplements, and in particular relates to a nutritional supplement for mixed food for pet kittens and a preparation method thereof. Background Art

[0002] The overall immune capacity of pet kittens is an important indicator of their health. The 4-12 weeks after a kitten is born is an immune blank period. During this period, the kitten is not protected by maternal antibodies, and its own immune system is not fully developed. It cannot produce enough antibodies to resist diseases, and may experience vomiting, diarrhea, fever and other discomfort symptoms, which may even be life-threatening in severe cases.

[0003] At present, there are various nutritional compositions for pet kittens on the market, which are intended to improve the immunity of pet kittens and beautify their hair. The main food products are added with ingredients including specific nutrients required for the immune system, natural antioxidants, prebiotics, immunoactive substances, etc., and the necessary support and protection are provided for the kittens during the immune blank period through daily feeding of the kittens to promote the healthy immune system development of the kittens. These ingredients can supplement additional nutrients for pet kittens and enhance their resistance and immunity. Hair problems are the characteristics of kittens that pet owners can observe intuitively. Among them, as the scarcity of kitten breeds increases, pet owners pay more attention to the hair of their pets, mainly to improve the quality and gloss of their hair. However, the existing nutritional compositions for mixing grains also have some major problems and shortcomings, mainly focusing on publicity and neglecting product quality; the ingredients are not suitable for the taste or preference of pet kittens; containing artificial additives, preservatives, pigments and other substances that are harmful to pet kittens, which may lead to overnutrition or undernutrition of pet kittens, low nutrient absorption efficiency, obesity, indigestion, lack of vitality and other problems, and will also cause hair problems, liver and kidney damage and other side effects.

[0004] Therefore, it is necessary to develop a natural and safe food-mixing nutritional composition that can improve the overall immunity of pet kittens and improve the hair condition. Summary of the invention

[0005] The purpose of the present invention is to provide a nutritional supplement for mixed food for pet kittens that improves the overall immunity of pet kittens and improves the condition of their hair, so as to solve the problem that pet cats in the weaning and early childhood periods whose immune systems are not yet fully developed cannot produce enough antibodies to resist diseases and are prone to inflammation of the body, which is specifically manifested as vomiting, diarrhea, ringworm and other discomfort symptoms. The nutritional supplement of the present invention has the characteristics of balanced nutrition, immunity enhancement, and natural safety. It can promote the growth and development of kittens in their infancy, increase the content of immune substances in serum, improve the intestinal health of kittens, skin psoriasis and frizzy hair problems, and achieve the effect of strengthening physical fitness and healthy development. At the same time, providing a method for preparing the nutritional supplement for mixed food for pet kittens is the second invention purpose of the present invention.

[0006] In order to achieve the above-mentioned invention object, the present invention adopts the following technical solutions:

[0007] A nutritional supplement for mixed food for pet kittens is composed of the following raw materials in parts by weight: 500-1000 parts of lactoferrin, 150-400 parts of fructooligosaccharides, 150-400 parts of mannooligosaccharides, 150-400 parts of inulin, 125 parts of yam extract, 100 parts of vitamin C, 50 parts of freeze-dried bovine spleen polypeptides, and 20-53 parts of probiotics.

[0008] As a further preferred embodiment of the present invention, the ratio of the total amount of fructooligosaccharide, mannooligosaccharide and inulin to lactoferrin is 1:1.

[0009] As a further preferred embodiment of the present invention, in the nutritional supplement, the probiotics include 0-20 parts of Bacillus subtilis, 0-10 parts of Bacillus licheniformis, 0-10 parts of Bifidobacterium lactis, 0-10 parts of Lactobacillus plantarum, and 0-3 parts of Bacillus coagulans.

[0010] The second invention object of the present invention is to provide a method for preparing the pet kitten food nutritional supplement, comprising the following steps:

[0011] 1) passing lactoferrin, fructooligosaccharide, mannooligosaccharide, inulin, yam extract, vitamin C, and freeze-dried bovine spleen polypeptide through a 100-mesh sieve, weighing each raw material in proportion, and mixing and stirring to obtain a mixture A; in this step, passing through a 100-mesh sieve ensures that the raw materials are finely ground, and the obtained raw materials are more delicate, so that the raw materials are mixed more evenly while ensuring that the powder is not easy to agglomerate.

[0012] 2) Mix the mixture A and the probiotics according to a certain proportion to obtain a nutritional supplement for kitten food.

[0013] As a further preferred embodiment of the present invention, in step 1), the production process of the yam extract is as follows: wash and peel the yam and slice it, freeze-dry it at -80℃ for 24h, dry it at 95℃ for 24h, crush it, add water and microwave extract it for 3h according to the material-liquid ratio of 1:5, collect the concentrate by rotary evaporation, freeze-dry it at -80℃ again, grind it into powder, and obtain the yam extract; the temperature of the microwave extraction is 95℃, and the power of the microwave extraction is 830W. Compared with the existing yam extract, adding the yam extract prepared by the method of the present invention can significantly increase the effective extraction content of the yam extract in the present invention, and the effective components of the present invention are more dense under the condition of the same yam extract addition content; in this step, freeze-drying at -80℃ and then drying at 95℃ can effectively and quickly achieve the purpose of dehydrating the raw materials, and achieve a better dehydration effect than freeze-drying alone. In addition, the energy consumption of freeze-drying is about ten times that of drying. Using this method can effectively reduce energy consumption and production costs.

[0014] The production process of the freeze-dried bovine spleen polypeptide is as follows: fresh bovine spleen is rinsed clean, fascia and fat are removed, and then the spleen is cut into pieces, blood is removed, and then the spleen is broken using a homogenizer, protease is added to enzymolyze the spleen for 2.5 hours, and then high-pressure steam sterilization is performed, freeze-dried for 48 hours using a freeze dryer at -80°C, and ground into powder to obtain freeze-dried bovine spleen polypeptide; the proteases are papain and trypsin, and the dosage of the papain and trypsin is 0.1 g / kg.

[0015] The nutritional supplement provided by the present invention contains lactoferrin, bovine spleen polypeptide, yam extract and a variety of prebiotics and probiotics. The raw materials are used in combination to significantly improve the overall immunity and hair condition of kittens, and are harmless to the health of pets. Among them, the lactoferrin used can improve the active immunity of the body to a certain extent. Lactoferrin was first separated from cow's milk, and its crystals are red. It has the ability to regulate macrophage activity and stimulate lymphocyte synthesis. Moreover, experiments show that lactoferrin has a certain effect on the increase of the proportion of immune cells in the blood of kittens.

[0016] The bovine spleen is rich in a variety of bioactive polypeptides, which are mainly derived from the catabolism of proteins in the spleen. One of the spleen aminopeptides can stimulate the immune system and enhance the body's resistance. It can promote the proliferation and differentiation of lymphocytes, increase the number of white blood cells, and improve the body's ability to resist pathogenic microorganisms, thereby playing a positive role in fighting diseases. Spleen aminopeptides have anti-inflammatory effects, can inhibit the occurrence of inflammatory reactions, and alleviate inflammatory symptoms and tissue damage caused by inflammation. The preparation method of freeze-dried bovine spleen polypeptides of the present invention can produce bovine spleen polypeptides simply and quickly, the method is simple and efficient, and the product yield is high, and the prepared product has high biological activity.

[0017] Prebiotics are a kind of food ingredients that cannot be digested and absorbed by animals. Prebiotics can provide nutrients for probiotics, can be used as food for beneficial bacteria in the intestine, promote its growth and reproduction, thereby can improve the intestinal microecological environment, increase the flora metabolites in the intestine. The present invention adopts the ratio of fructooligosaccharide, mannooligosaccharide, inulin to lactoferrin as 1:1, can significantly increase the number of bifidobacteria and acidophilus in the intestine, inhibit the infection of pathogenic bacteria such as Escherichia coli and Salmonella, increase the short-chain fatty acids in the intestine, improve intestinal barrier function, improve constipation and diarrhea, and improve immunity.

[0018] Yam extract is a natural plant active substance extracted from yam. It contains rich ingredients that can improve the digestion and absorption ability of kittens and prevent the occurrence of digestive tract diseases such as indigestion, diarrhea, and constipation; it can promote growth and development, regulate endocrine, delay aging, and promote hair growth; it can also regulate the mental state of kittens and relieve their anxiety, fear, tension and other negative emotions.

[0019] The probiotics of the present invention are composed of a variety of different probiotics, each of which has a different effect. They can create suitable growth conditions for other beneficial bacteria by consuming oxygen in the intestine, and can also secrete a variety of enzymes, vitamins, coagulants, etc., which can lower the pH value of the intestine, which is beneficial to the colonization and growth of beneficial bacteria in the intestine, enhance the stability of the intestinal flora, inhibit the growth of harmful bacteria, improve immunity, promote the repair and regeneration of intestinal epithelial cells, enhance the intestinal barrier function, prevent intestinal inflammation and infection, and promote nutrient absorption.

[0020] Compared with the prior art, the present invention has the following beneficial effects:

[0021] 1. The present invention is a natural, safe and nutritious supplement product developed according to the taste and preference of pet kittens. It is mainly aimed at weaning and young cats whose immune systems are not fully developed and cannot produce enough antibodies to resist diseases. They are prone to inflammation problems with symptoms such as vomiting, diarrhea, psoriasis, etc.

[0022] 2. The freeze-dried bovine spleen polypeptide, yam extract and vitamin C added in the present invention can reduce the proportion of diarrhea while improving the quality of feces of kittens, achieving a soft, formed and moist feces state without cracks and with a clear shape. At the same time, it can also achieve the effect of increasing the proportion of immune cells in the blood of kittens and improving the overall immunity of kittens;

[0023] 3. Experiments show that the present invention can effectively improve the overall immunity of kittens while improving the hair condition of kittens, and has obvious characteristics in applicable animal groups, animal age groups and composition formulas. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] In order to more clearly illustrate the technical solution of the present invention, the following is a brief introduction to the drawings required for use in the embodiments. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without paying any creative work.

[0025] Figure 1 It is the result of the routine blood test of kittens (a. total white blood cell count, b. white blood cell ratio, c. lymphocytes, d. granulocytes);

[0026] Figure 2 It is the serum biochemical index of kittens (a. aspartate aminotransferase b. alanine aminotransferase c. creatine kinase d. urea nitrogen / creatinine e. total bilirubin);

[0027] Figure 3 Feed intake and weight gain of kittens in the experimental group and the control group (a. feed intake b. daily weight gain);

[0028] Figure 4 These are the scoring results of kittens in the experimental group and the control group (a. mental state score, b. coat color score, c. feces score);

[0029] Figure 5 is the change in test results between the experimental group, the control group and comparative example 1 (a. total white blood cell count);

[0030] Figure 6 is the change of test results between the experimental group, the control group and comparative example 2 (a. lymphocytes);

[0031] Figure 7 The test results of Example 1 and Comparative Example 3 are as follows (a. powder content b. feed intake);

[0032] Figure 8 It is the stool scoring result of Examples 1-3 and Comparative Example 4. DETAILED DESCRIPTION

[0033] In order to make the purpose, technical solutions and advantages of the present invention more clear, the present invention is further described below in conjunction with specific implementation methods.

[0034] Examples 1-5

[0035] To make the description concise and easier to understand, the raw material compositions of Examples 1-5 are given in table form below, as shown in Table 1:

[0036] Table 1 Composition of raw materials in Examples 1-5

[0037]

[0038] The preparation method of the above-mentioned embodiments 1-5 comprises the following steps:

[0039] 1) passing lactoferrin, fructooligosaccharide, mannooligosaccharide, inulin, yam extract, vitamin C, and freeze-dried bovine spleen polypeptide through a 100-grit sieve and weighing each raw material in proportion, mixing and stirring to obtain a mixture A;

[0040] 2) Mix the mixture A and the probiotics according to a certain proportion to obtain a nutritional supplement for kitten food.

[0041] In this embodiment, in step 1), the production process of the Chinese yam extract is: wash and peel the Chinese yam and slice it, freeze-dry it at -80℃ for 24h, dry it at 95℃ for 24h, crush it, add water according to the material-liquid ratio of 1:5 for microwave extraction for 3h, collect the concentrate by rotary evaporation, freeze-dry it at -80℃ again, grind it into powder, and obtain the Chinese yam extract; the temperature of the microwave extraction is 95℃, and the power of the microwave extraction is 830W. The production process of the freeze-dried bovine spleen polypeptide is: rinse the fresh bovine spleen, cut it into pieces after removing the fascia and fat, crush it with a homogenizer after removing the blood, add protease to enzymolyze the bovine spleen for 2.5h, sterilize it with high pressure steam, freeze-dry it at -80℃ for 48h using a freeze dryer, grind it into powder, and obtain freeze-dried bovine spleen polypeptide; in this embodiment, the protease is papain and trypsin, and the amount of papain and trypsin added to each 1kg of bovine spleen is 0.1g.

[0042] Application Example: Effect of the present invention on immunity and hair of pet kittens

[0043] 1. Experimental Animals

[0044] Six kittens from the same batch were bred by the same mother cat. All six kittens had just been weaned, and there were no significant differences in their initial body weight and hair condition.

[0045] 2. Experimental Grouping

[0046] Six kittens were randomly divided into two groups and fed with the same staple food. The control group was fed with only the staple food without nutritional supplements. The experimental group was fed with 500g of the nutritional supplement Example 1 formula 1 package mixed evenly. There were three kittens in each group.

[0047] 3. Feeding and management

[0048] The cats were fed in a single cage and weighed on the 0th, 14th and 28th day of the experiment. Six trained professionals were also recruited to score the coat color, mental state and fecal status. Every morning, cat feces, cat cages and water basins were cleaned regularly, and clean drinking water was replaced. The experimental cats exercised properly outside the cat cages for 1 hour every day, and their food intake was recorded. On the 28th day, blood was collected from the left forelimb vein of each pet cat for routine blood tests and blood biochemical index tests.

[0049] The scoring criteria are shown in Table 2-4 and the test results are shown in Figure 1-4 .

[0050] Scoring rules:

[0051] Table 2 Hair status scoring criteria

[0052]

[0053]

[0054] Table 3 Mental status scoring criteria

[0055]

[0056] 15-18 points: The cat is in a very good mental state, has strong immunity, is healthy, is in a good mood, and is in a good mental state. 10-14 points: The cat is in a relatively good mental state, has strong immunity, is basically healthy, is in a stable mood, and is in a normal mental state.

[0057] 5-9 points: The cat's mental state is average, its immune ability is average, it may have slight physical discomfort, its mood may fluctuate, there are some physical problems, and its mental state is not good.

[0058] 0-4 points: The cat's mental state is poor, its immune system is weak, it may be obviously uncomfortable, its mood may be depressed, its body is severely damaged, and its mental state is extremely poor.

[0059] Table 4 5-point stool scoring scale standard

[0060] grade Rating Basis 1 Hard, dry, small, brittle lumps that crumble when lightly squeezed 1.5 The feces have visible cracks, are extremely dry on the outside, relatively dry on the inside, and leave no residue on the ground when picked up 2 Hard, formed, dry feces, with only a small amount remaining on the ground when picked up, and no deformation 2.5 The stool is moist and slightly deformed when picked up 3 Soft, formed, moist stools without cracks, with a clear shape, and that lose their shape when picked up 3.5 The stool is moist, thin and unformed. 4 Soft, unformed stools that are moist 4.5 Watery stools with low viscosity 5 The stool is completely liquid

[0061] From Table 2-4 and Figure 1-4 It can be concluded from the data that after 28 days of feeding, the experimental group of kittens had no significant increase in feed intake and daily weight gain compared with the control group. The white blood cell ratio in the whole blood increased significantly, indicating that the kittens absorbed nutrients better after adding it. The content of white blood cells, lymphocytes, and granulocytes increased, indicating that the number of white blood cells in the blood of kittens increased, and it had a certain effect on the increase in the proportion of immune cells. The content of aspartate aminotransferase, alanine aminotransferase, creatine kinase, urea nitrogen / creatinine, and total bilirubin in the blood decreased, indicating that after adding it, liver damage and muscle damage can be effectively reduced; kidney damage, jaundice index, and the chance of viral infection and bacterial infection can be effectively reduced. At the same time, after adding nutritional supplements, the kittens' hair condition, mental state, and fecal status such as psoriasis were intuitively improved.

[0062] It can be concluded from the data that the number of white blood cells in the blood of the kittens in the test group increased compared with the control group when the nutritional supplement of the present invention was mixed with grain. The present invention has a certain effect on the increase of the proportion of immune cells. The kittens in the test group can also effectively reduce liver damage and muscle damage compared with the control group; effectively reduce kidney damage, jaundice index, and reduce the probability of viral infection and bacterial infection. The kittens in the test group have improved indigestion, constipation and diarrhea compared with the control group, and can relieve the anxiety, fear, tension and other negative emotions of the kittens, indicating that the feeding of the present invention can achieve the effect of improving the overall immunity of the kittens.

[0063] The hair growth of kittens in the experimental group was significantly improved compared to the control group, and problems such as frizzy hair and psoriasis were effectively alleviated.

[0064] Comparative Example 1

[0065] The nutritional supplement of this comparative example is the same as that of Example 1, except that the yam extract, freeze-dried bovine spleen polypeptide and vitamin C are removed from the nutritional supplement of this comparative example; 500 parts of lactoferrin, 200 parts of fructooligosaccharides, 150 parts of mannooligosaccharides, 150 parts of inulin, 20 parts of Bacillus subtilis, 10 parts of Bacillus licheniformis and 3 parts of Bacillus coagulans are included.

[0066] Figure 5 The test results of Example 1 are changed (a. total white blood cell count). At the same time, no nutritional supplement is added as a control. Compared with the present invention, the total white blood cell count of Example 1 is similar to that of the control group without nutritional supplements, and is significantly lower than that of the test group of Example 1. In addition, the fecal score is also significantly lower than that of Example 1, indicating that after removing the yam extract, freeze-dried bovine spleen polypeptide and vitamin C, pet kittens have varying degrees of constipation and diarrhea.

[0067] Comparative Example 2 Effect of too low lactoferrin dosage on the present invention

[0068] The nutritional supplement of this comparative example is the same as that of Example 1, except that the mass ratio of lactoferrin to the mass ratio of yam extract and prebiotics in the nutritional supplement of this comparative example is different; specifically:

[0069] 100 parts of lactoferrin, 200 parts of fructooligosaccharide, 150 parts of mannooligosaccharide, 150 parts of inulin, 125 parts of yam extract, 50 parts of freeze-dried bovine spleen polypeptide, 100 parts of vitamin C, 20 parts of Bacillus subtilis, 10 parts of Bacillus licheniformis, and 3 parts of Bacillus coagulans.

[0070] Figure 6The test results of Example 2 are changed (a. lymphocytes). At the same time, no supplement is added as a control. Compared with Example 1 of the present invention, the lymphocytes of Example 2 are similar to those of the control group without using nutritional supplements, and are significantly higher than the test group of Example 1, indicating that the anti-inflammatory effect of Example 2 is poor; in addition, aspartate aminotransferase is also significantly higher than that of Example 1, indicating that when the amount of lactoferrin added is too low, it cannot effectively alleviate the damage to liver function.

[0071] Comparative Example 3 Effect of Too High Lactoferrin Dosage on the Present Invention

[0072] The nutritional supplement of this comparative example is the same as that of Example 1, except that the mass ratio of lactoferrin to the mass ratio of yam extract and prebiotics in the nutritional supplement of this comparative example is different; specifically:

[0073] 1500 parts of lactoferrin, 200 parts of fructooligosaccharide, 150 parts of mannooligosaccharide, 150 parts of inulin, 125 parts of yam extract, 50 parts of freeze-dried bovine spleen polypeptide, 100 parts of vitamin C, 20 parts of Bacillus subtilis, 10 parts of Bacillus licheniformis, and 3 parts of Bacillus coagulans.

[0074] The influence of excessive lactoferrin dosage on the present invention is shown in Figure 7 As shown, Figure 7 The test results of powder content and feed intake of Example 1 and Comparative Example 3 are as follows. Figure 7 It can be seen that excessive dosage of lactoferrin will significantly increase the powder content in the staple food, causing the stability of the product to decrease. At the same time, it will affect the taste of pet cats, reduce the palatability of pet cats and the amount of food intake in the same period of time.

[0075] Comparative Example 4 Effect of different amounts of prebiotics on the present invention

[0076] On the basis of Example 1, the dosage of prebiotics was changed to 150 parts of fructooligosaccharide, 150 parts of mannooligosaccharide, and 150 parts of inulin, and compared with Examples 1, 2, and 3.

[0077] Figure 8 is the stool scoring result of Examples 1-3 and Comparative Example 4, from Figure 8 It can be seen that the feces score of Comparative Example 3 is significantly higher than that of Example 1, Example 2, and Example 3, indicating that after feeding the formula of Comparative Example 4, more obvious soft stools and diarrhea occurred, indicating that the dosage of added prebiotics is too low to inhibit the growth of Escherichia coli and Salmonella in the intestine.

[0078] The preferred embodiments of the present invention are specifically described above, but the present invention is not limited to the embodiments. Those skilled in the art may make various equivalent modifications or substitutions without violating the spirit of the present invention, and these equivalent modifications or substitutions are all within the scope defined by the claims of this application. Those skilled in the art should regard the specification as a whole, and the technical solutions in the embodiments may also be appropriately combined to form other implementation methods that can be understood by those skilled in the art.

Claims

1. A nutritional supplement for pet kittens, characterized in that: The invention is composed of the following raw materials in parts by weight: 500-1000 parts of lactoferrin, 150-400 parts of fructooligosaccharide, 150-400 parts of mannooligosaccharide, 150-400 parts of inulin, 125 parts of yam extract, 100 parts of vitamin C, 50 parts of freeze-dried bovine spleen polypeptide, and 20-53 parts of probiotics; The ratio of the total amount of fructooligosaccharide, mannooligosaccharide and inulin to lactoferrin is 1:1; In the nutritional supplement, the probiotics include 20 parts of Bacillus subtilis, 10 parts of Bacillus licheniformis, 0-10 parts of Bifidobacterium lactis, 0-10 parts of Lactobacillus plantarum, and 3 parts of Bacillus coagulans.

2. The method for preparing the nutritional supplement for pet kittens according to claim 1, characterized in that: The following steps are involved: 1) passing lactoferrin, fructooligosaccharide, mannooligosaccharide, inulin, yam extract, vitamin C, and freeze-dried bovine spleen polypeptide through a 100-grit sieve and weighing each raw material in proportion, mixing and stirring to obtain a mixture A; 2) Mixing the mixture A and the probiotics in proportion to obtain a nutritional supplement for kitten food; In step 1), the preparation process of the yam extract is as follows: wash and peel the yam and slice it, freeze-dry it at -80°C for 24 hours, dry it at 95°C for 24 hours, crush it, add water at a material-liquid ratio of 1:5, perform microwave extraction for 3 hours, collect the concentrate by rotary evaporation, freeze-dry it at -80°C again, grind it into powder, and obtain the yam extract; the temperature of the microwave extraction is 95°C, and the power of the microwave extraction is 830W; In step 1), the production process of the freeze-dried bovine spleen polypeptide is as follows: rinse the fresh bovine spleen, remove the fascia and fat, cut into pieces, remove the blood and use a homogenizer to break it, add protease to enzymolyze the bovine spleen for 2.5 hours, then sterilize it with high-pressure steam, use a freeze dryer at -80°C to freeze-dry for 48 hours, grind it into powder, and obtain freeze-dried bovine spleen polypeptide; the protease is papain and trypsin, and the amount of papain and trypsin is 0.1g / kg.

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