Application of a sugarcane SsJMJ11 gene in regulating plant drought resistance

Abstract

The present invention relates to the field of biotechnology, and in particular to an application of a sugarcane SsJMJ11 gene in regulating plant drought resistance, wherein the SsJMJ11 gene sequence is shown in SEQ ID NO.1. The protein sequence encoded by the SsJMJ11 gene is shown in SEQ ID NO.2. The present invention analyzes the wild sugarcane species S. serrata with strong stress resistance and finds that multiple histone modifications of sugarcane change significantly under drought stress, wherein the expression level of the H3K4me1 / 2 / 3 demethylase gene SsJMJ11 changes significantly under drought stress, indicating that it responds to drought stress and has the potential to regulate plant drought resistance. Through plant genetic engineering technology, a CDS fragment of the complete coding region of SsJMJ11 was cloned from the S. serrata SES208 material, and the function of the gene was verified, and it was found that heterologous overexpression of SsJMJ11 resulted in reduced drought resistance of Arabidopsis transgenic materials, and significantly increased stomata sensitivity to ABA, indicating that it plays a negative regulatory role in drought. It can be seen that SsJMJ11 has important application value in the directional genetic improvement of plant drought resistance and the breeding of new drought-resistant varieties.

Description

Technical Field

[0001] The invention relates to the field of biotechnology, and in particular to an application of a sugarcane SsJMJ11 gene in regulating plant drought resistance. Background Art

[0002] Terrestrial plants may be affected by a variety of external stresses during their growth and development, including abiotic stresses such as drought, salinity, and low temperature. Plants cannot escape adverse environments by moving like animals, so they have evolved a series of strategies to resist adverse stresses. Among them, plant drought resistance has always been a research hotspot in the field of botany, and traditional breeding techniques have certain difficulties in improving stress tolerance traits. With the development of molecular biology technology and in-depth research on the molecular mechanisms of plant drought resistance, molecular-level stress resistance genetic engineering has made significant progress. Introducing drought-resistant exogenous genes into plants through genetic engineering methods such as transgenics has become one of the new ways to improve plant drought resistance. Therefore, it is particularly important to explore and identify genetic resources that improve plant drought resistance.

[0003] After a long process of adaptive evolution in the natural environment, plants have evolved a variety of strategies to resist or avoid drought stress and reduce the damage caused by water shortage. Studies have found that the expression of drought stress response genes is closely related to their histone modification and the degree of relaxation of nucleosomes. After drought treatment, the methylation modification of histone H3K4 changes dramatically, and the expression of a large number of genes changes, indicating that the dynamic regulation of histone methylation modification plays an important role in plant drought response. Among them, a histone demethylase family gene encoding a JmjC domain protein, the enzyme it encodes can specifically eliminate the histone methylation modification of drought response genes to resist drought stress. For example, overexpression of H3K4 demethylase OsJMJ703 in rice will weaken drought resistance, while mutation of OsJMJ703 will enhance the drought resistance of rice. Arabidopsis thaliana H3K9 demethylase JMJ27 positively regulates the plant's response to drought stress by regulating drought response genes RD20 and GOLS2. In addition, H3K36 demethylase OsJMJ710 negatively regulated drought stress by inhibiting the expression of MYB48-1 in rice. It can be seen that histone demethylase genes can be used as potential gene resources to improve plant drought resistance. Summary of the invention

[0004] The purpose of the present invention is to solve the shortcomings of the prior art and propose an application of the sugarcane SsJMJ11 gene in regulating plant drought resistance, to achieve artificial regulation and improvement of plant drought resistance through genetic engineering methods, and to discover the SsJMJ11 gene with the ability to regulate plant drought resistance from sugarcane.

[0005] In order to achieve the above object, the present invention adopts the following technical solutions:

[0006] An application of a sugarcane SsJMJ11 gene in regulating plant drought resistance, wherein overexpression of the SsJMJ11 gene can reduce the drought resistance of Arabidopsis thaliana;

[0007] The SsJMJ11 gene is the SsJMJ11 gene of the cut hand secret SES208.

[0008] Preferably, the nucleotide sequence of the SsJMJ11 gene is as shown in SEQ ID NO.1.

[0009] Preferably, the protein sequence encoded by the SsJMJ11 gene is shown in SEQ ID NO.2.

[0010] Preferably, the overexpression specifically comprises: introducing a recombinant expression vector containing the SsJMJ11 gene into Arabidopsis thaliana.

[0011] Preferably, the recombinant expression vector is obtained by inserting the CDS sequence of the SsJMJ11 gene into the cloning site of pSUPER:eGFP.

[0012] Among them, the above-mentioned SsJMJ11 gene or the protein encoded by the SsJMJ11 gene is used as a negative regulatory factor in regulating plant drought resistance and / or reducing plant sensitivity to ABA, and the plant is a transgenic Arabidopsis plant overexpressing the SsJMJ11 gene.

[0013] Among them, negative regulation is manifested as follows: under drought stress, compared with the control plants, the drought resistance of SsJMJ11 transgenic plants is significantly lower than that of the control plants, and their sensitivity to ABA is lower than that of the control plants.

[0014] By adopting the above technical scheme: the present invention analyzes wild sugarcane species with strong drought resistance and finds that multiple histone modifications of sugarcane change significantly under drought stress, and the expression level of histone demethylase gene SsJMJ11 changes significantly under drought stress. Through phenotypic experiments, it is found that reducing the expression of SsJMJ11 gene can enhance plant drought resistance. It can be seen that the SsJMJ11 gene isolated from sugarcane materials with strong drought resistance can be used as an important gene resource to improve plant drought resistance, and has the potential for application in crop drought resistance breeding.

[0015] Compared with the prior art, the present invention has the following beneficial effects:

[0016] The present invention provides a novel gene showing adjustable plant drought resistance and exhibiting negative regulation drought response characteristics; the purpose of improving plant drought resistance can be achieved by knocking out the gene, namely the sugarcane SsJMJ11 gene. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 This is a schematic diagram of the expression of the SsJMJ11 gene in response to drought stress (RT-qPCR);

[0018] (a) Phenotype of S. sutchuenensis seedlings in response to drought stress under RSWC. RSWC, relative soil water content. Scale bar, 5 cm. (b) RT-qPCR analysis of SsJMJ11 expression in leaves, roots, and stems in response to drought. (c) RT-qPCR analysis of SsJMJ11 expression in response to various abiotic stresses. SseEF is an internal reference gene, all data are mean ± SE (n = 3), and the number of * indicates a significant difference in one-way ANOVA performed with T test (*P < 0.05, **P < 0.01).

[0019] Figure 2 The schematic diagram of the protein domain of SsJMJ11 and SsJMJ11m genes of the present invention and the schematic diagram of the homology analysis of the KDM5 / JARID1 histone sequence in the cut hand secretion;

[0020] (a) Schematic diagram of the protein domains of SsJMJ11 and SsJMJ11m, H308A and E310A indicate that the predicted Fe(II) binding amino acids (His and Glu) are replaced by Ala. (b) The conserved amino acids associated with the demethylation activity of the α-KG binding site are highlighted in arrows, and the conserved amino acids in the Fe(II) binding site are highlighted in stars. The protein encoded by SsJMJ11 has 586 amino acids. JmjC is the histone demethylation protein domain.

[0021] Figure 3 It is the subcellular localization map of the SsJMJ11 gene of the present invention;

[0022] (a) Subcellular localization of pSUPER:GFP and pSUPER:SsJMJ11-GFP 48h after Agrobacterium infection of Nicotiana benthamiana leaves. From left to right, pSUPER:SsJMJ11-GFP green fluorescence, DAPI nuclear localization signal, bright field image, and the fusion effect of the first three. The marked yellow arrow indicates the cell nucleus. (b) Subcellular localization of pSUPER:GFP and pSUPER:SsJMJ11-GFP in sugarcane protoplasts. From left to right, GFP fluorescence signal, nuclear localization marker, bright field, and mixed image. mCherry-ARF191V is a nuclear localization marker with RFP signal. The image scale is 10μm.

[0023] Figure 4 Schematic diagram of the detection of H3K4 demethylase activity of SsJMJ11 in vivo of the present invention;

[0024] (a) SsJMJ11 exhibits H3K4me1 / 2 / 3 demethylase activity in vivo. (b) Mutations in the conserved Fe(II)-binding amino acids (SsJMJ11m, H308A, and E310A) abolish its H3K4me1 / 2 / 3 demethylase activity in vivo. Blue, green, and red fluorescence indicate DAPI-stained nuclei, SsJMJ11 / SsJMJ11m-GFP, and H3K4me1 / 2 / 3 modifications, respectively. White and yellow arrows indicate nuclei expressing and not expressing SsJMJ11 / SsJMJ11m-GFP, respectively. Image scale is 10 μm. (c) Quantification of methylation marks shown in (a) and (b). More than 60 pairs of SsJMJ11 / SsJMJ11m-GFP expressing nuclei were counted. The number of * indicates that there is a significant difference in one-way analysis of variance using T test (*P<0.05, **P<0.01).

[0025] Figure 5 Schematic diagram of the selection of the SsJMJ11 transgenic stable strain and the detection of its histone modification level in the present invention;

[0026] (a) RT-qPCR detection of SsJMJ11 gene expression in Arabidopsis overexpression lines. UBC was used as an internal reference gene, and all data are mean ± SE (n = 3). (b) Anti-GFP was used to detect the protein expression levels of Col-0, EV (pSUPER: GFP), overexpression lines SsJMJ11 OE (pSUPER: SsJMJ11-GFP) and SsJMJ11mOE (pSUPER: SsJMJ11m-GFP). Anti-H3K4me1, anti-H3K4me2, and anti-H3K4me3 were used to detect the H3K4me1 / 2 / 3 modification levels of Col-0, EV, overexpression lines SsJMJ11 OE and SsJMJ11m OE, and H3 histone was used as an internal reference protein.

[0027] Figure 6 This is a schematic diagram of the drought stress response of Arabidopsis thaliana negatively regulated by SsJMJ11 of the present invention;

[0028] (a) Phenotype of 14-day-old seedlings subjected to continuous soil drought treatment and three days of drought rewatering. Data represent mean ± SE (n = 6). (b) Plant survival rate after three days of drought rewatering. (c) 4-week-old seedlings subjected to continuous in vitro water loss for 10 hours, and the hourly water loss rate of plants was calculated. Data represent mean ± SE (n = 6). (de) Stomatal density and stomatal index of the abaxial epidermis of leaves of 4-week-old EV, SsJMJ11 OE and SsJMJ26 OE plants. Data are shown as mean ± SD (n = 10). (f) Stomatal aperture in response to ABA. Scale bar, 10 μm. (g) Quantification of stomatal closure response to ABA in EV, SsJMJ11 OE and SsJMJ26 OE transgenic plants. Data represent mean ± SD (n = 60). All different letters indicate that there is a significant difference (P < 0.05) in one-way analysis of variance performed by T test. DETAILED DESCRIPTION

[0029] The technical solutions in the embodiments of the present invention will be described clearly and completely below in conjunction with the accompanying drawings, so that those skilled in the art can better understand the advantages and features of the present invention, thereby making a clearer definition of the protection scope of the present invention. The embodiments described in the present invention are only a part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments in the present invention, all other embodiments obtained by ordinary technicians in the field without making creative work are within the scope of protection of the present invention.

[0030] Unless otherwise specified, the experimental methods used in the following examples are conventional methods.

[0031] Unless otherwise specified, the materials and reagents used in the following examples can be obtained from commercial sources.

[0032] The pSUPER:eGFP vector, Arabidopsis thaliana, Columbia ecotype, and Nicotiana benthamiana wild type have been disclosed in the literature "Guangrun Yu, Daoqian Chen, Meiling Ye, et al. (2024). H3K27 demethylase SsJMJ4 negatively regulates drought-stress responses in sugarcane. Journal of Experimental Botany." and are available to the public from the Straits Joint Research Institute of Fujian Agriculture and Forestry University.

[0033] Saccharum spontaneum L SES208 has been published in the document "Qiongli Wang, Guangrun Yu, Zhiyong Chen, et al (2021). Optimization of protoplast isolation, transformation and its application in sugarcane (Saccharum spontaneum L). Crop Journal." and is available to the public from the Straits Joint Research Institute of Fujian Agriculture and Forestry University.

[0034] The brand of EZ-Flex Seamless Assembly and Cloning Kit is GenStar, and the product number is T195-20.

[0035] Example 1: Inhibition of SsJMJ11 gene expression under drought stress

[0036] 1. Primer Design and Synthesis

[0037] Real-time quantitative fluorescence PCR primers were designed according to the CDS sequence of the SsJMJ11 gene. The primer sequences are:

[0038] Forward Primer:

[0039] 5'-TCGAGTGAGGACTCCGAAGA-3'

[0040] Reverse Primer:

[0041] 5'-CGCCCTCAATTTCCTCCACT-3'

[0042] 2. Drought stress treatment of SES208 plants

[0043] Take SES208 growing in the field, cut it into stems of about 6-8cm with one internode and buds, wash it clean and place it in a large container, add carbendazim and soak it overnight, wash the carbendazim, place it in a glass bottle with water, put two to three stems in each glass bottle, place it in a 30℃ vernalization box for about ten days, and wait for the buds to grow to about 15cm.

[0044] Drought material treatment with different soil moisture contents. Select seedlings with consistent growth and move them to pots with a diameter of 26 cm, cultivate them with the same weight of nutrient soil, and place them in a greenhouse. When the seedlings grow to about ten days old, select seedlings with consistent growth for drought treatment. One group is watered normally as a control, and the other three groups are subjected to drought treatment. Samples are taken at three soil moisture contents of 20%, 15%, and 10%.

[0045] Total RNA was extracted from the above materials, and cDNA was obtained by reverse transcription. Real-time quantitative fluorescence PCR was performed using the above DNA molecules as primers. The results are shown in Figure 1 shown.

[0046] Figure 1 The results showed that different drought conditions could inhibit the expression of SsJMJ11. The transcription level of SsJMJ11 was inhibited in all tested tissues, and the inhibition level in leaves and stems was stronger than that in roots ( Figure 1 b).

[0047] Example 2: Cloning of SsJMJ11 and SsJMJ11m genes, schematic diagram of protein domains, and homology analysis of the conserved JmjC domain sequences of the KDM5 / JARID1 subfamily in S. sutchuenensis.

[0048] 1. Primer Design and Synthesis

[0049] Primers with Gateway adapters were designed based on the CDS sequence of the SsJMJ11 gene. The primer sequences are:

[0050] Forward Primer:

[0051] pSUPER:SsJMJ11-HindIII-F:

[0052] 5'-aaatcgactctagaaagcttATGCTTTCCACATCCGCCGAGG-3'

[0053] Reverse Primer:

[0054] pSUPER:SsJMJ11-KpnI-R:

[0055] 5'-tgctcaccatggtaccGGTGGTCCTGTGATCCTGTCCC-3'

[0056] Primers with Gateway adapters were designed based on the CDS sequence of the SsJMJ11m gene. The primer sequences are:

[0057] Forward Primer:

[0058] pSUPER:SsJMJ11m-HindIII-F:

[0059] 5'-aaatcgactctagaaagcttATGCTTTCCACATCCGCCGAGG-3'

[0060] SsJMJ11m-F(Overlap):5'-TTTGCTGGGCTGTTGCAGAC-3'

[0061] Reverse Primer:

[0062] pSUPER:SsJMJ11-KpnI-R:

[0063] 5'-tgctcaccatggtaccGGTGGTCCTGTGATCCTGTCCC-3'

[0064] SsJMJ11m-R(Overlap):5'-GTCTGCAACAGCCCAGCAAA-3'

[0065] 2. The total RNA of S. japonica SES208 was extracted, and cDNA was obtained by reverse transcription. PCR amplification was performed using the cDNA as a template and the DNA molecules shown above as primers to obtain a PCR amplification product (as shown in SEQ ID No.1). The CDS sequence of the SsJMJ11 gene is shown in SEQ ID No.1 from the 1st to the 1758th base from the 5' end, and the SsJMJ11 protein sequence is shown in SEQ ID No.2 from the 1st to the 586th nucleotide from the 5' end.

[0066] 3. After gel recovery of the PCR amplification product containing the SsJMJ11 fragment, the Gateway cloning expression kit (EZ-Flex Seamless Assembly and Cloning Kit) was used to connect the pSUPER:eGFP restriction vector large fragment to obtain a recombinant plasmid, which was named pSUPER:SsJMJ11-GFP. pSUPER:SsJMJ11-GFP was subjected to Sanger sequencing, and the sequence alignment result was correct, and the amino acid sequence containing the JmjC domain was obtained ( Figure 2 a).

[0067] Fourth, the sequence homology analysis of the conserved JmjC domain of the KDM5 / JARID1 subfamily in the cut hand secretion showed that the JmjC protein domain of SsJMJ11 has the conserved amino acid sequence for binding enzyme activity auxiliary factors Fe(II) and α-KG, just like AtJMJ14 / 15 / 16 / 18 and OsJMJ703 / 704, indicating that SsJMJ11 may have H3K4 demethylase activity ( Figure 2 b).

[0068] According to the protein domain diagram of SsJMJ11 and SsJMJ11m genes, the same vector construction method was used to mutate the 308th His amino acid and the 310th Glu conservative amino acid of the Fe(II) binding site that produces demethylation activity to Ala amino acid to construct the SsJMJ11 loss-of-function mutation vector pSUPER:SsJMJ11m-GFP( Figure 2 ).

[0069] Example 3: Subcellular localization of the SsJMJ11 gene

[0070] 1. Subcellular localization and protein expression of SsJMJ11 in tobacco

[0071] The pSUPER:SsJMJ11-GFP plasmid obtained in Example 2 was transformed into GV3101 Agrobacterium, and the pSUPER:SsJMJ11-GFP plasmid was transferred into Nicotiana benthamiana by Agrobacterium infection. The pSUPER:GFP empty plasmid was used as a control. The leaves of the overexpressing tobacco were selected for DAPI staining after 48 hours of culture and observed under a laser confocal microscope. The results are as follows: Figure 3 As shown in a, from left to right are pSUPER:SsJMJ1-GFP green fluorescence, nuclear localization signal, bright field image, and the fusion effect of the first three. The marked yellow arrow indicates the cell nucleus.

[0072] 2. Subcellular localization of SsJMJ11 in sugarcane protoplasts

[0073] The pSUPER:SsJMJ11-GFP plasmid and the pSUPER:GFP empty vector were transformed into the protoplasts of the young leaf tissue of SES208 respectively, and the results were observed under a laser confocal microscope. Figure 3 b. From left to right are GFP fluorescence signal, nuclear localization marker, bright field, and mixed image. mCherry-ARF191V is a nuclear localization marker with RFP signal. The image scale is 10 μm. Figure 3 The results showed that SsJMJ11 was mainly expressed in the cell nucleus. Histone methylation modification occurs in the cell nucleus, which lays the foundation for SsJMJ11 to function as a possible H3K4 demethylase in the cell nucleus.

[0074] Example 4: Detection of H3K4 demethylase activity of SsJMJ11

[0075] The tobacco leaves after SsJMJ26 overexpression for 48 h were obtained in the same manner as in Example 3. The cell nuclei were extracted and then immunofluorescence experiments were performed. The results were observed under a laser confocal microscope. Figure 4As shown. Blue, green, and red fluorescence indicate DAPI-stained cell nuclei and SsJMJ11 / SsJMJ11m-GFP and H3K4 modification, respectively. White and yellow arrows indicate cell nuclei expressing and not expressing SsJMJ11 / SsJMJ11m-GFP, respectively. The image scale is 10 μm. Figure 4 The results showed that SsJMJ11 has H3K4me1 / 2 / 3 demethylase activity in vivo, and mutations in the conserved Fe(II)-binding amino acids (SsJMJ11m, H308A, and E310A) abolished its H3K4me1 / 2 / 3 demethylase activity in vivo, which laid the foundation for subsequent functional studies.

[0076] Example 5: Effect of SsJMJ11 on plant drought resistance

[0077] 1. Obtaining SsJMJ11 overexpressing plants

[0078] The transformed strain obtained in Example 1 was transformed into GV3101 Agrobacterium, and the pSUPER:SsJMJ11-GFP plasmid was transferred into Col-0 by Agrobacterium infection, and the pSUPER:GFP empty plasmid was transferred as a control. The transfected plants were screened on a 1 / 2MS medium containing 30 mg / L hygromycin to obtain homozygous positive plants with hygromycin resistance. The obtained transgenic plants were subjected to DNA detection, quantitative detection, protein expression detection and H3K4 modification detection.

[0079] The expression levels of SsJMJ11 detected in the transgenic lines transformed with empty Arabidopsis and pSUPER:SsJMJ11-GFP vectors are shown in Figure 2. Figure 5 As shown in a, SsJMJ11 OE#9 and #10 had the highest expression levels compared with the empty Arabidopsis, and the complete SsJMJ11 protein was detected using anti-GFP. The corresponding histone modifications of transgenic plants were detected by anti-H3K4me1, anti-H3K4me2, and anti-H3K4me3 antibodies, and H3 histone was used as the internal reference protein. The results showed that SsJMJ11 has the ability to remove H3K4me1 / 2 / 3 modifications. Therefore, the T3 generation SsJMJ11 OE#9-3 and #10-2 homozygous strains of SsJMJ11 OE#9 and #10 were selected for subsequent drought resistance phenotype analysis, and the UBC gene was used as the internal reference to ensure that the starting amount of cDNA was consistent.

[0080] 2. Analysis of drought phenotypes in SsJMJ11 overexpressing plants

[0081] Transgenic lines SsJMJ11 OE#9-3 and #10-2 and Arabidopsis thaliana (EV) with empty plasmid were cultured on 1 / 2MS solid medium. After one week, the seedlings were transplanted to soil for growth and continuous soil drought was performed to compare drought resistance. The results are shown in the figure. Figure 6 As shown in a.

[0082] The results showed that the overexpression of SsJMJ11 gene made the transgenic lines SsJMJ11OE#9-3 and #10-2 more sensitive to drought than those of Arabidopsis thaliana with no transgenic lineage, and the survival rate after rehydration was lower ( Figure 6 b), indicating that the SsJMJ11 gene negatively regulates plant drought resistance.

[0083] Effect of SsJMJ11 overexpression on water loss in detached leaves of transgenic lines

[0084] After the transgenic Arabidopsis thaliana SsJMJ11 OE#9-3 and #10-2 and the untransfected Arabidopsis thaliana were grown in soil for 2 weeks, rosette leaves of the same weight were cut and subjected to an in vitro leaf water loss experiment. The water loss rate results are shown in Figure 6 As shown in c. Figure 6 These results indicate that overexpression of SsJMJ11 can negatively regulate the drought resistance of plants and increase the water loss rate of detached leaves.

[0085] Effect of SsJMJ11 overexpression on stomatal aperture of transgenic lines

[0086] After transgenic Arabidopsis SsJMJ11 OE#9-3 and #10-2 and empty-load Arabidopsis were grown in soil for 2 weeks, rosette leaves of the same weight were cut and incubated in stomatal opening solution (10 mM MES, pH 6.15, 50 mM CaCl2, 10 mM KCl) at 22 °C for 3 h, and then incubated in 5 μM ABA solution for 3 hours. For stomatal density analysis, the epidermis was obtained from the distal 1 / 3 of leaves of comparable size. The epidermis was imaged using an Olympus BX63 microscope. Stomatal density and stomatal index are shown in Figure 2. Figure 6 d and e. Subsequently, the epidermis was peeled off and placed on a glass slide. Stomatal aperture was imaged using an Olympus BX63 microscope and measured using Image J software. Figure 6 f and g. Figure 6 The results showed that SsJMJ11 was insensitive to ABA-induced stomatal closure, indicating that under drought stress conditions, SsJMJ11 may reduce the plant's adaptability to drought stress by inhibiting stomatal closure.

[0087] In summary, the present invention analyzes the wild sugarcane species S. japonica with strong stress resistance and finds that various histone modifications of sugarcane change significantly under drought stress, among which the expression level of H3K4me1 / 2 / 3 demethylase gene SsJMJ11 changes significantly under drought stress and other abiotic stresses. Through plant genetic engineering technology, the CDS fragment of the complete coding segment of SsJMJ11 was cloned from the S. japonica SES208 material, and the function of the gene was verified. It was found that heterologous overexpression of SsJMJ11 led to reduced drought resistance of Arabidopsis transgenic materials, and the sensitivity of stomata to ABA was significantly increased, indicating that it plays a negative regulatory role in drought. It can be seen that SsJMJ11 has the potential to be used in gene editing to cultivate new drought-resistant varieties, and is of great significance to the directional genetic improvement of plant drought resistance and the breeding of new drought-resistant varieties.

[0088] The description and practice disclosed in the present invention are easy to think and understand for ordinary technicians in the technical field, and several improvements and modifications can be made without departing from the principles of the present invention. Therefore, modifications or improvements made without departing from the spirit of the present invention should also be regarded as the protection scope of the present invention.

Claims

1. A kind of sugarcane SsJMJ11 The application of a gene in regulating plant drought resistance is characterized in that: Overexpression of the SsJMJ11 Genes can reduce the drought resistance of plants, SsJMJ11 Gene is SES208 SsJMJ11 Gene; The plant is Arabidopsis thaliana; Said SsJMJ11 The nucleotide sequence of the gene is shown in SEQ ID NO.

1.

2. The use according to claim 1, characterized in that: Said SsJMJ11 The amino acid sequence of the protein encoded by the gene is shown in SEQ ID NO.2.

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