A traditional Chinese medicine composition with hypoglycemic effect and a preparation method thereof

Abstract

The present application provides a traditional Chinese medicine composition with blood sugar lowering efficacy and a preparation method thereof, and belongs to the technical field of traditional Chinese medicines; the traditional Chinese medicine composition is prepared from the following raw materials in parts by mass: birch juice 140-200 parts, jujube fruit 20-40 parts, rehmannia 20-40 parts, adenosma 18-26 parts, periploca 18-26 parts, ophiopogon 18-26 parts, bamboo shavings 12-19 parts, leech 12-19 parts, psyllium 12-19 parts, dogwood 10-14 parts, gordon euryale seed 10-14 parts. In the traditional Chinese medicine composition, the blood sugar lowering effect is mainly achieved by repairing the function of islet beta cells, promoting the regeneration of islet beta cells, improving sugar metabolism, increasing the sensitivity of insulin or improving insulin resistance, so that the overall prescription achieves the effects of invigorating the spleen and lung, nourishing yin and reducing fire, benefiting the stomach and producing saliva, astringing the spleen essence, promoting transportation and transformation, tonifying the kidney and strengthening the body, and relieving polydipsia and restlessness.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of traditional Chinese medicine, in particular to a traditional Chinese medicine composition with blood sugar lowering effect and a preparation method thereof. BACKGROUND

[0002] With the acceleration of people's life pace, people's living and diet are more and more irregular, and various diseases are also entangling people. Especially the middle-aged and elderly population, the number of people suffering from high blood pressure, high blood lipids and high blood sugar is increasing year by year, and these diseases seriously endanger people's physical and mental health. High blood sugar can induce diabetes, which is one of the diseases that people are difficult to overcome, and brings great pain to the daily life of patients. Diabetes is a chronic systemic endocrine and metabolic disease, and sustained high blood sugar not only leads to abnormal metabolism of some tissues and organs, but also causes dysfunction and morphological changes. The symptoms caused by these changes include microvascular complications such as microcirculation disorder, microaneurysm formation and thickening of microvascular basement membrane, and the most terrible thing for diabetic patients is complications. Common complications of diabetes are diabetic nephropathy and diabetic retinopathy, which can lead to uremia and blindness in severe cases. At present, most of the drugs for treating high blood sugar adopt western medicine treatment, and these drugs have single treatment effect, and have poor western medical effect, large side effects and high treatment cost; some traditional Chinese medicines treat symptoms but not the root cause, and do not treat the root cause. Therefore, there is an urgent need in the market for a traditional Chinese medicine composition widely applicable to blood sugar lowering. SUMMARY

[0003] Therefore, the present application aims to provide a traditional Chinese medicine composition with blood sugar lowering effect and a preparation method thereof.

[0004] To achieve the above-mentioned purpose, the present application provides the following technical solutions:

[0005] The present application provides a traditional Chinese medicine composition with blood sugar lowering effect, which is prepared from the following raw materials in parts by mass:

[0006] Birch juice 140-200 parts, jujube fruit 20-40 parts, rehmannia 20-40 parts, radix adnateglabrae 18-26 parts, radix pseudostellariae 18-26 parts, ophiopogon 18-26 parts, bamboo shavings 12-19 parts, leeches 12-19 parts, plantago seeds 12-19 parts, and dogwood 10-14 parts.

[0007] Preferably, the dosage form of the traditional Chinese medicine composition is at least one selected from tablets, granules, capsules, traditional Chinese medicine pills, drop pill preparations, powders, syrups and mixtures.

[0008] Preferably, it further comprises a pharmaceutically acceptable excipient.

[0009] The present application also provides a preparation method of the traditional Chinese medicine composition, comprising the following steps:

[0010] 1) washing, drying and crushing all raw materials except birch juice to obtain mixed raw materials;

[0011] 2) extracting the mixed raw materials by hot refluxing with ethanol solution twice, combining the extract, filtering and recovering ethanol, and collecting the first extract and residue;

[0012] 3) mixing the residue with birch juice, and then soaking, decocting and filtering to obtain the second extract;

[0013] 4) combining the second extract with the first extract to obtain the traditional Chinese medicine composition.

[0014] Preferably, the particle size of the crushed material in step 1) is 60-100 mesh.

[0015] Preferably, the mass ratio of the mixed raw materials to the ethanol solution in step 2) is 1:6-10.

[0016] Preferably, the refluxing temperature of the ethanol solution in step 2) is 55-75℃, and the extraction time is 1-2h.

[0017] Preferably, the soaking time in step 3) is 20-30min.

[0018] Preferably, the decocting time in step 3) is 40-60min.

[0019] Preferably, the decocting temperature in step 3) is 55-70℃.

[0020] The present application has the following beneficial technical effects:

[0021] Birch juice is the fresh juice flowing out from the incision of the bark or the bore of the trunk of birch, which has the effects of invigorating the stomach and protecting the spleen, clearing heat and detoxifying according to Bencao Gangmu; it is called in Bencaohuiyan that "birch juice is bitter and cold, non-toxic, and has the effects of clearing heat and dampness, expelling phlegm and relieving cough, and detoxifying"; hovenia dulcis has the effect of diuresis and detumescence, and it contains rich hovenia dulcis polysaccharides, which can improve insulin sensitivity, help control blood sugar rise, promote insulin secretion, and assist in reducing blood sugar, etc. Rehmannia glutinosa has the functions of clearing heat and cooling blood, and nourishing yin and generating fluid, and the polysaccharides contained therein can promote the secretion of insulin and improve insulin sensitivity; flavonoids can inhibit the absorption and digestion of sugar and reduce the rise of blood sugar; proteins can increase the release of insulin and reduce blood sugar level. The above three are the monarch drug, which can reduce blood sugar level while clearing heat and detoxifying, nourishing yin and generating fluid.

[0022] Adopting the traditional Chinese medicine composition, the traditional Chinese medicine composition can lower blood sugar while tonifying liver and kidney and protecting the spleen and stomach, and the lowering of blood sugar is mainly through repairing the function of pancreatic beta cells, promoting the regeneration of pancreatic beta cells, improving sugar metabolism, increasing the sensitivity of insulin or improving insulin resistance, so that the whole prescription can achieve the effects of invigorating the spleen and lung, nourishing yin and reducing fire, benefiting the stomach and producing saliva, condensing the essence of the spleen, promoting transportation and transformation, tonifying the kidney and strengthening the body, and relieving polydipsia and polyuria, and is widely applicable to diabetes.

[0023] The bamboo shoot has the effects of clearing heat and reducing phlegm, relieving restlessness and vomiting, and preventing miscarriage. Research proves that the bamboo shoot has the effects of enhancing the body's immunity, resisting tumors, reducing blood sugar and blood lipids, protecting the liver, reducing weight, promoting blood circulation, and delaying aging. The plant protein and polysaccharide substances contained in the bamboo shoot have the effect of reducing blood sugar, can promote the secretion and utilization of insulin, and adjust the blood sugar level. At the same time, the trace elements and vitamins in the bamboo shoot can reduce the synthesis of cholesterol and triglycerides, reduce the fat content in the blood, thereby playing a role in reducing blood pressure. The leech has the effects of breaking blood, unblocking channels, and removing stasis. The leech contains various alkaloids, such as hirudin and astragaloside, which have the effect of assisting in reducing blood sugar. The plantain seed has the effects of clearing heat, diuresis, unblocking the urinary tract, stopping diarrhea, improving eyesight, and removing phlegm. The plantain seed contains some active ingredients, such as flavonoids and polysaccharides, which have the effect of assisting in reducing blood sugar. The above three ingredients are used as auxiliary medicines, which can assist in reducing blood sugar while protecting the liver.

[0024] The cornus officinalis has the effects of tonifying liver and kidney and astringing and consolidating. At the same time, the cornus officinalis contains ursolic acid, which has the effect of reducing blood sugar and urine sugar. The euryale ferox has the effects of invigorating the spleen, stopping diarrhea, tonifying the kidney, astringing, moistening the intestines, unblocking the urinary tract, and tonifying the kidney and heart. The above two ingredients are used as ministerial medicines, which can invigorate the spleen and tonify the kidney, astringe and consolidate while assisting in reducing blood sugar.

[0025] The traditional Chinese medicine composition can lower blood sugar while tonifying liver and kidney and protecting the spleen and stomach, and the lowering of blood sugar is mainly through repairing the function of pancreatic beta cells, promoting the regeneration of pancreatic beta cells, improving sugar metabolism, increasing the sensitivity of insulin or improving insulin resistance, so that the whole prescription can achieve the effects of invigorating the spleen and lung, nourishing yin and reducing fire, benefiting the stomach and producing saliva, condensing the essence of the spleen, promoting transportation and transformation, tonifying the kidney and strengthening the body, and relieving polydipsia and polyuria, and is widely applicable to diabetes.

[0026] The traditional Chinese medicine composition adopts the hot reflux low-temperature extraction technology, simple operation, less solvent, low extraction temperature, effectively prevents the destruction of bioactive components in pressure or high temperature extraction, ensures the stability of quality, and the medicine residues are boiled again with birch juice, the active ingredients of the medicine are extracted to the maximum, and the product hypoglycemic effect is improved. DETAILED DESCRIPTION

[0027] In order to better understand the present application, the content of the present application is further illustrated below in combination with examples, but the content of the present application is not limited to the following examples.

[0028] Example 1

[0029] A traditional Chinese medicine composition with blood sugar lowering effect, the traditional Chinese medicine composition is prepared from the following mass fraction of raw materials:

[0030] Birch juice 140 parts, jujube 20 parts, rehmannia 20 parts, saussurea 18 parts, psoralea 18 parts, maimendong 18 parts, bamboo 12 parts, leech 12 parts, plantain seed 12 parts, dogwood 10 parts, gordon euryale 10 parts;

[0031] The preparation method of the traditional Chinese medicine composition, the steps are as follows:

[0032] 1) All the raw materials except birch juice are washed, dried and crushed to 60 mesh to prepare mixed raw materials;

[0033] 2) The mixed raw materials are extracted twice by hot refluxing with ethanol solution, and then the extract is combined and filtered in turn, and the ethanol is recovered, and the first medicine liquid and the residue are collected; the mass ratio of mixed raw materials to ethanol solution is 1:6; the temperature of each ethanol refluxing is 55℃, and the extraction time is 1h;

[0034] 3) The residue is mixed with birch juice, soaked for 20min, then 55℃ decocting for 40min, filtering to prepare the second medicine liquid;

[0035] 4) The second medicine liquid and the first medicine liquid are combined to prepare the traditional Chinese medicine composition of the present application.

[0036] Example 2

[0037] A traditional Chinese medicine composition with blood sugar lowering effect, the traditional Chinese medicine composition is prepared from the following mass fraction of raw materials:

[0038] Birch juice 200 parts, jujube 40 parts, rehmannia 40 parts, saussurea 26 parts, psoralea 26 parts, maimendong 26 parts, bamboo 19 parts, leech 19 parts, plantain seed 19 parts, dogwood 14 parts, gordon euryale 14 parts;

[0039] The preparation method of the traditional Chinese medicine composition, the steps are as follows:

[0040] 1) wash, dry and grind all the raw materials except birch juice to 100 mesh to prepare mixed raw materials;

[0041] 2) extract the mixed raw materials by hot refluxing with ethanol solution twice, combine the extract and filter and recover ethanol successively, collect the first extract and residue; the mass ratio of mixed raw materials to ethanol solution is 1:10; the temperature of each time of ethanol refluxing is 75℃, and the extraction time is 2h;

[0042] 3) mix the residue with birch juice, soak for 30min, then decoct at 70℃ for 60min, and filter to prepare the second extract;

[0043] 4) combine the second extract with the first extract to prepare the traditional Chinese medicine composition.

[0044] Example 3

[0045] A traditional Chinese medicine composition with blood sugar lowering effect, which is prepared from the following raw materials in mass parts:

[0046] 180 parts of birch juice, 30 parts of jujube fruit, 30 parts of rehmannia, 22 parts of adiantum capillus-veneris, 22 parts of periploca, 22 parts of ophiopogon, 15 parts of bamboo, 15 parts of leech, 15 parts of plantago seed, 12 parts of cornus, and 12 parts of gordon euryale;

[0047] The preparation method of the traditional Chinese medicine composition, the steps are as follows:

[0048] 1) wash, dry and grind all the raw materials except birch juice to 80 mesh to prepare mixed raw materials;

[0049] 2) extract the mixed raw materials by hot refluxing with ethanol solution twice, combine the extract and filter and recover ethanol successively, collect the first extract and residue; the mass ratio of mixed raw materials to ethanol solution is 1:8; the temperature of each time of ethanol refluxing is 65℃, and the extraction time is 1.5h;

[0050] 3) mix the residue with birch juice, soak for 25min, then decoct at 60℃ for 50min, and filter to prepare the second extract;

[0051] 4) combine the second extract with the first extract to prepare the traditional Chinese medicine composition.

[0052] Experimental Example 1

[0053] 1. Test materials

[0054] Male mice, weighing 21±3g, were selected. The test instrument included a blood glucose meter and a syringe. The test reagent was alloxan. The product obtained in the method of Example 3 was used as the experimental group (the use method was 120mg / kg / d by gavage); the prior art group was metformin (the use dose was 80mg / kg / d).

[0055] 2. Test method

[0056] 2.1 Establishment of alloxan diabetic mouse model

[0057] After the mice were adaptively fed for 7 days, they were fasted (without water restriction) for 12h, and venous blood was taken to detect the blood glucose concentration. The high blood glucose model was established by injecting alloxan at a concentration of 1% in two doses, with the first dose being higher than the second dose, and 5% glucose was supplemented 2h after injection.

[0058] 2.2 Grouping

[0059] The test mice were randomly divided into 3 groups, 10 mice in each group, namely the model group, the experimental group, and the prior art group.

[0060] 2.3 Treatment method

[0061] The model group, the experimental group, and the prior art group were gavaged with the same volume of the corresponding drugs, and the normal group was gavaged with the same volume of normal saline. The observation lasted for 4 weeks, and the fasting blood glucose was detected regularly.

[0062] 2.4 Blood glucose detection in normal mice

[0063] Thirty mice were taken and randomly divided into 3 groups, namely the model group, the experimental group, and the prior art group. Each group was gavaged with the same volume of the corresponding drugs every day, and the normal group was gavaged with the same volume of normal saline, once a day for 28 consecutive days. Venous blood was taken regularly to detect the blood glucose.

[0064] 2.5 Blood glucose detection in high blood glucose mice

[0065] Thirty mice were taken and randomly divided into 3 groups, namely the model group, the experimental group, and the prior art group. Alloxan (260mg / kg) was injected intraperitoneally to cause a diabetic model. The mice were fasted for 12h before injection, and venous blood was taken 72h after injection to determine the blood glucose. Animals that did not cause the model (fasted for 12h, blood glucose less than 11.1mmol / L) were removed. Each group was gavaged in the same way as 2.4, once a day for 28 consecutive days, and venous blood was taken regularly to detect the blood glucose.

[0066] 3. Test results

[0067] The results are shown in Table 1. There was no significant difference in the blood glucose values of the mice in each group after administration compared with the normal group; there was no significant difference in the blood glucose values of the mice in each group after administration compared with the blood glucose values before administration.

[0068] Table 1 Influence on blood sugar of normal mice

[0069] 0d 7d 14d 21d 28d model group 5.32 5.48 5.70 5.64 5.83 experimental group 5.37 5.71 5.69 5.38 5.10 prior art group 5.33 5.56 5.37 5.34 5.02

[0070] As shown in Table 2, before treatment, there was no significant difference in blood sugar value among groups; on day 0, the fasting blood sugar of each diabetes group was very high; on day 7, the blood sugar of the experimental group and the prior art group decreased significantly (p<0.05); on day 14 and thereafter, the blood sugar of the two groups decreased more significantly. Therefore, the product prepared by the present application can reduce the blood sugar of the alloxan-induced hyperglycemic mice to a certain extent, thereby improving the symptoms of the alloxan-induced diabetic mice.

[0071] Table 2 Influence on blood sugar of diabetic mice

[0072]

[0073]

[0074] The above only describes the preferred embodiments of the present application, and it should be noted that, for those skilled in the art, without departing from the principles of the present application, a number of improvements and refinements can be made, and these improvements and refinements should also be considered as the protection scope of the present application.

Claims

1. A traditional Chinese medicine composition having hypoglycemic efficacy, characterized in that, The traditional Chinese medicine composition is prepared from raw materials in the following mass fractions: Birch juice 180 parts, Hovenia acerba 30 parts, Rehmannia glutinosa 30 parts, Adenophora stricta 22 parts, Cortex Lycii 22 parts, Ophiopogon japonicus 22 parts, Bamboo shavings 15 parts, Leech 15 parts, Plantago seed 15 parts, Cornus officinalis 12 parts, Euryale ferox 12 parts; The preparation method of the traditional Chinese medicine composition is as follows: 1) All raw materials except birch juice are sequentially washed, dried, and crushed to 80 meshes to obtain mixed raw materials; 2) The mixed raw materials are extracted twice by hot refluxing with an ethanol solution, the extract is combined, and sequentially filtered and ethanol is recovered, and the first medicinal liquid and medicinal residue are collected; the mass ratio of the mixed raw materials to the ethanol solution is 1:8; the temperature of each time of ethanol refluxing is 65 DEG C, and the extraction time is 1.5 h; 3) The medicinal residue is mixed with the birch juice, soaked for 25 min, then decocted at 60 DEG C for 50 min, and filtered to obtain the second medicinal liquid; 4) The second medicinal liquid is combined with the first medicinal liquid to obtain the traditional Chinese medicine composition.

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