Method for high branch layering propagation of malmea fruit

Abstract

The application belongs to the technical field of propagation of Malpighia emarginata, and particularly relates to a Malpighia emarginata cutting propagation method. The Malpighia emarginata cutting propagation method comprises the following steps: S1. selecting a branch of Malpighia emarginata plant crown periphery which is developed and healthy, and cutting the branch into a 30-40cm branch segment; S2. immersing the lower end of the branch segment into rooting liquid for 10s, and then taking out the branch segment and inserting the branch segment into a translucent non-woven fabric bag filled with growth substrate; S3. placing the non-woven fabric bag into a transparent box, covering the box cover, and then transferring the non-woven fabric bag to a shady place without direct sunlight; S4. after 1 month, the branch segment sprouts new shoots, and fungicide is sprayed on the leaf surface once, and then the spraying is performed once every month; S5. when the roots are fully grown and the new shoot branches and leaves are mature, the box cover is gradually opened, the cuttings are gradually exposed to the external environment, and transplanting is performed. The propagation method can effectively prevent the influence of Botrytis cinerea on the survival rate of the branch segments, is beneficial to guaranteeing the survival rate and propagation efficiency of the branch segments, and is further beneficial to the smooth development of Malpighia emarginata seedling raising work.

Description

TECHNICAL FIELD

[0001] The application belongs to the technical field of propagation of Euterpe precatoria, and particularly relates to a high-branch layering propagation method of Euterpe precatoria. BACKGROUND

[0002] Euterpe precatoria, also known as Mommy Fruit, is a new type of fruit originally from a South American country. Euterpe precatoria is a evergreen large tree which can be used for greening and economic fruit trees. The fresh pulp of the fruit can be eaten, and can also be used for ice cream and fruit juice. Euterpe precatoria can be propagated by high-branch layering and cutting. The cutting propagation can retain the excellent properties of the female parent. The cutting is also called a cutting, which is a common propagation method for cultivating plants. The stems, roots, leaves and buds of plants can be cut and inserted into the soil or soaked in water. After rooting, the cutting can be planted to become an independent new plant.

[0003] In the cutting seedling process of Euterpe precatoria, the Euterpe precatoria is also affected by diseases and pests. The gray mold caused by Botrytis cinerea is a common one. The gray mold on the cutting seedlings of Euterpe precatoria seriously affects the survival rate of the eucalyptus seedlings. In production, chemical agents are commonly used to prevent and control the gray mold. However, long-term use of a single chemical pesticide can easily cause the pathogenic bacteria to be resistant. In order to improve the prevention and control effect, the dosage of the pesticide is usually increased, which can cause a series of problems such as resistance, pesticide residues and environmental pollution.

[0004] Chinese patent CN201410205194.9 discloses the application of a-antrodin in the prevention and treatment of plant diseases. The chemical structural formula of a-antrodin is disclosed in the patent. It is also disclosed that a-antrodin has a good inhibitory effect on a variety of plant pathogenic bacteria such as mango collar rot fungus, mango anthracnose fungus, tomato gray mold fungus and corn large spot fungus.

[0005] The compounding of pesticide active ingredients with different mechanisms is an effective and quick way to develop new pesticide products and prevent and control resistant pests. The compounding of different pesticide active ingredients can improve the prevention and control effect, reduce the amount of pesticide used, and delay the generation of pest resistance. There is no related report on the compounding of a-antrodin and pyraclostrobin.

[0006] The information disclosed in the background section of this document is only intended to increase the understanding of the overall background of the application and should not be considered as acknowledging or implying in any form that the information constitutes prior art known to those skilled in the art. SUMMARY

[0007] The purpose of this invention is to provide a method for propagating Mammillaria rubra by high-branch layering, which can effectively prevent gray mold from occurring during the branch segment cutting process and facilitate the smooth progress of Mammillaria rubra seedling cultivation.

[0008] To achieve the above objectives, the present invention provides the following technical solution:

[0009] The first objective of this invention is to provide a bactericidal composition comprising a binary compound of α-dextrin and azoxystrobin, wherein the mass ratio of α-dextrin to azoxystrobin is 1-10:7.5-1.

[0010] Preferably, the mass ratio of α-dextrin to azoxystrobin is 1:7.5.

[0011] Secondly, the present invention also provides a bactericide, wherein the bactericide is prepared by using the bactericidal composition of claim 1 as the active ingredient, supplemented with a dispersant, a wetting agent and a filler.

[0012] Preferably, the bactericide contains the following components by mass percentage: 15-30% active ingredient, 6-8% dispersant, 3-10% wetting agent, and the remainder is filler.

[0013] Preferably, the dispersant is sodium lignosulfonate, the wetting agent is sodium dodecyl sulfate, and the filler is attapulgite.

[0014] Finally, the present invention also provides a method for propagating Mami fruit by cuttings, comprising the following steps:

[0015] S1. Select healthy branches from the outer canopy of the Ma Meiguo plant, cut them into 30-40cm branches, trim the leaves of the branches, leaving only the petioles and 3-4 leaves, and cut the lower end of the branches into a 45° cut.

[0016] S2. Immerse the lower end of the branch segment in the rooting solution for 10 seconds, then remove it and insert it into a semi-transparent non-woven bag filled with growth substrate to a depth of 5-6 cm.

[0017] S3. Place the non-woven bag into a transparent box, close the lid, and move it to a cool, shady place away from direct sunlight.

[0018] S4.1 months later, new shoots emerge from the branch segments. The foliar surface is sprayed with the fungicide described in claim 4 once, and then once a month thereafter.

[0019] S5. Once the roots have fully developed and the new shoots and leaves have matured, gradually open the box lid at night or on rainy days to allow the cuttings to gradually enter the external environment and then transplant them.

[0020] Compared with the prior art, the present invention has the following beneficial effects:

[0021] The fungicide of this invention contains α-dextrin and azoxystrobin, which have a synergistic effect on the pathogen of gray mold, improving the control effect against gray mold. This can largely prevent the occurrence of gray mold during the branch cutting process, effectively preventing the impact of gray mold pathogens on the survival rate of branch cuttings, and helping to ensure the survival rate and propagation efficiency of branch cuttings, thus facilitating the smooth development of Ma Meiguo seedling cultivation. Detailed Implementation

[0022] The present invention can be better understood from the following embodiments. However, those skilled in the art will readily understand that the descriptions in the embodiments are for illustrative purposes only and should not, and will not, limit the invention as detailed in the claims.

[0023] Example 1 Activity test of α-dextrin and azoxystrobin combined with botrytis cinerea as the pathogen of gray mold.

[0024] 1. Test strain: Botrytis cinerea, collected from the leaves of infected jujube trees and isolated in the laboratory.

[0025] 2. Culture medium: Potato dextrose agar (PDA) medium (200g potato, 20g glucose, 18g agar, 1000mL water) is used to culture the pathogen.

[0026] 3. Test reagents: 98% α-dextrin technical grade (CAS: 6147-11-1), 95% azoxystrobin technical grade, both of which are commercially available.

[0027] 4. Preparation of drug-containing plates: The test reagent was first dissolved in dimethyl sulfoxide, then diluted with 0.1% Tween-80 aqueous solution to prepare single-agent stock solutions. Multiple formulations were prepared, with five mass concentration gradients for each single agent and formulation mixture, using a proportional method. 9 mL of pre-melted PDA medium was added to a sterile Erlenmeyer flask. 1 mL of the drug solution was then quantitatively pipetted from low to high concentration and added to the respective Erlenmeyer flasks. After thorough mixing, the solutions were poured into 9 cm diameter petri dishes to prepare drug-containing plates of the corresponding concentrations.

[0028] 5. Toxicity Assay: Following the guidelines for indoor bioassay testing of pesticides, the mycelial growth rate method was used to determine toxicity. The test strains were propagated on PDA plates and cultured at 28℃ for 3 days. Mycelial discs with a diameter of 5 mm were aseptically punched along the edge of the colony and inoculated into the center of the drug-containing plate. The plates were then incubated at 28℃. Each treatment was repeated three times, with a control treatment (without pesticide) as a blank control. After 3 days of culture, the colony diameter for each treatment was measured using the cross-sectional method. The average colony diameter was calculated, and the inhibition rate of mycelial growth by different treatments was calculated.

[0029]

[0030] 6. Data Processing: Using the logarithm of the fungicide concentration as the toxicity regression equation x, and the corresponding mycelial growth inhibition rate probability value as the toxicity regression equation y, a linear regression was performed to obtain the toxicity regression equation and the EC50 of the fungicide. 50 The values ​​were calculated, and the co-toxicity coefficient (CTC) was calculated according to Sun Yunpei's method. The synergistic effect of the agent was evaluated based on the calculated CTC. CTC≤80 indicates antagonistic effect, 80<CTC<120 indicates additive effect, and CTC≥120 indicates synergistic effect. The results are shown in Table 1.

[0031] Table 1. Indoor bioactivity assays of α-dextrin and azoxystrobin combined with gray mold pathogens.

[0032]

[0033]

[0034] Table 1 shows that α-dextrin and azoxystrobin have a synergistic effect on Botrytis cinerea, the pathogen of gray mold, within a mass ratio of 1-10:7.5-1. The synergistic effect is most pronounced at a mass ratio of 1:7.5, where the co-toxicity coefficient reaches a certain level.

[0035] In summary, the combination of α-dextrin and azoxystrobin in the fungicide of this invention has a synergistic effect on the pathogen of gray mold, which can improve the control effect of gray mold and thus largely avoid the occurrence of gray mold during the branch segment cutting process. It effectively prevents the impact of gray mold pathogen on the survival rate of branch segment cuttings, which is conducive to ensuring the survival rate and propagation efficiency of branch segment cuttings, and thus facilitates the smooth development of Ma Meiguo seedling cultivation.

[0036] Example 2

[0037] A method for propagating Mammillaria elegans by cuttings includes the following steps:

[0038] S1. Select healthy branches from the outer canopy of the Ma Meiguo plant, cut them into 30-40cm branches, trim the leaves of the branches, leaving only the petioles and 3-4 leaves, and cut the lower end of the branches into a 45° cut.

[0039] S2. Immerse the lower end of the branch segment in the rooting solution for 10 seconds, then remove it and insert it into a semi-transparent non-woven bag filled with growth substrate to a depth of 5-6 cm.

[0040] S3. Place the non-woven bag into a transparent box, close the lid, and move it to a cool, shady place away from direct sunlight.

[0041] S4.1 months later, new shoots will emerge from the branch segments. Spray the leaves with fungicide once, and then spray once a month thereafter.

[0042] S5. Once the roots have fully developed and the new shoots and leaves have matured, gradually open the box lid at night or on rainy days to allow the cuttings to gradually enter the external environment and then transplant them.

[0043] In step S4, the fungicide is prepared using a binary compound of α-dextrin and azoxystrobin as active ingredients, supplemented with dispersant sodium lignosulfonate, wetting agent sodium dodecyl sulfate, and filler attapulgite. The mass ratio of α-dextrin to azoxystrobin is 1:7.5. More specifically, the mass percentage of each component in the fungicide is: active ingredient 25%, dispersant 6%, wetting agent 8%, and the remainder is filler.

[0044] The foregoing description of specific exemplary embodiments of the invention is for illustrative and explanatory purposes. These descriptions are not intended to limit the invention to the precise forms disclosed, and it will be apparent that many changes and variations can be made in accordance with the foregoing teachings. The exemplary embodiments were chosen and described in order to explain the specific principles of the invention and its practical application, thereby enabling those skilled in the art to implement and utilize various different exemplary embodiments of the invention, as well as various different choices and variations. The scope of the invention is intended to be defined by the claims and their equivalents.

Claims

1. A bactericidal composition, characterized in that, The bactericidal composition is a binary compound of α-dextrin and azoxystrobin, wherein the mass ratio of α-dextrin to azoxystrobin is 1-10:7.5-1.

2. The bactericidal composition according to claim 1, characterized in that, The mass ratio of α-dextrin to azoxystrobin is 1:7.

5.

3. A bactericide, characterized in that, The bactericide is prepared using the bactericidal composition of claim 1 as the active ingredient, supplemented with dispersants, wetting agents and fillers.

4. The bactericide according to claim 3, characterized in that, The mass percentage of each component in the bactericide is as follows: 15-30% active ingredient, 6-8% dispersant, 3-10% wetting agent, and the remainder is filler.

5. The bactericide according to claim 4, characterized in that, The dispersant is sodium lignosulfonate, the wetting agent is sodium dodecyl sulfate, and the filler is attapulgite.

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