Preparation method of baidoupi pill

Abstract

The application relates to a preparation method of Bile Aralia cordata, wherein the filtrate of Coptis chinensis, Gentiana scabra and Lily is added according to a proportion, the mutual action between drugs and the synergistic effect in the human body are utilized, the bile Aralia cordata with good drug efficacy is prepared by using a low amount of bile, compared with the prior art, the amount of bile used is reduced, the cost of traditional Chinese medicine is reduced, and the harm to animals is reduced.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of traditional Chinese medicine processing and preparation, in particular to a preparation method of Bannao. BACKGROUND

[0002] Bannao is made of fine powder of Arisaema cum biles of ox, sheep or pig, or made of fine powder of raw Arisaema cum biles of ox, sheep or pig. Bannao is in the shape of square or cylinder; brown yellow, gray brown or black brown; hard in quality; slightly smelly and bitter in taste. Bannao is made of biles of ox, sheep or pig, so it contains bile acid components such as cholic acid, deoxycholic acid, hyodeoxycholic acid and sodium taurocholate. It has the effects of clearing heat and reducing phlegm, calming wind and relieving convulsion. It is mainly used for treating phlegm-heat cough, yellow and thick phlegm, wind-phlegm coma and epilepsy. The biles of ox, sheep or pig are best for processing, and the ratio of bile to Arisaema is about 4-8:1. However, the biles of ox, sheep or pig are not only expensive, but also cause great harm to animals in the process of bile extraction, which is inhumane. It is the focus of the field to study how to reduce the amount of bile while maintaining the efficacy of Bannao or to develop a new preparation method with better efficacy. However, the prior art does not have related achievements. The Chinese patent document with publication number CN109316539A discloses a Bannao and its preparation process, which adds sweet potato powder, lotus root powder, orange peel powder, hawthorn powder, turmeric powder, panax notoginseng powder and celery seed powder, but cannot solve the aforementioned problems. Therefore, in view of the problems in the prior art, it is urgent to provide a technology for preparing Bannao with good efficacy by compounding other medicinal materials with low bile content. SUMMARY

[0003] The purpose of the present application is to avoid the shortcomings of the prior art and provide a preparation method of Bannao with good efficacy by compounding other medicinal materials with low bile content.

[0004] The purpose of the present application is achieved by the following technical solutions:

[0005] A preparation method of Bannao is provided, which comprises the following steps:

[0006] (1) crushing Arisaema into powder;

[0007] (2) decocting Coptis chinensis, Gentiana scabra and Lilium brownii in water according to a weight ratio of 3:3:1, and filtering to obtain medicinal liquid;

[0008] (3) stirring Arisaema powder, bile and medicinal liquid uniformly according to a weight ratio of 1:2-3:1;

[0009] (4) placing the mixture of step (3) in a fermentation barrel for fermentation;

[0010] (5) drying or baking the fermented Bannao into shape and then slicing.

[0011] Preferably, in step (1), the Arisaema cum Bile is crushed into powder, and the fine Arisaema cum Bile powder is obtained by screening through an 80-100 mesh sieve.

[0012] Preferably, in step (2), the total weight of the Coptis chinensis, the Gentiana scabra and the Lily is 1:25-50 by weight of the water.

[0013] Preferably, in step (2), the decocting time is 25-60 minutes.

[0014] Preferably, in step (2), the decocting time is 30 minutes.

[0015] Preferably, in step (4), the fermentation time is 5-9 days.

[0016] Preferably, in step (4), the fermentation time is 7 days.

[0017] Preferably, in step (4), the fermentation barrel is kept at a constant temperature of 28-38℃ for fermentation.

[0018] Preferably, in step (4), the fermentation barrel is kept at a constant temperature of 30℃ for fermentation.

[0019] Advantages of the present application:

[0020] The present application adds the Coptis chinensis, the Gentiana scabra and the Lily filtrate according to the proportion, utilizes the mutual action between the drugs and the synergistic effect in the human body, realizes the preparation of the Arisaema cum Bile with good drug efficacy by using a small amount of bile, reduces the cost of traditional Chinese medicine under the condition of ensuring the same or better drug efficacy of the Arisaema cum Bile, and reduces the harm to animals. DETAILED DESCRIPTION

[0021] The present application is further described in combination with the following examples. Example 1

[0022] The preparation method of the Arisaema cum Bile in the present embodiment includes the following main steps:

[0023] (1) crushing Arisaema cum Bile into powder; and obtaining fine Arisaema cum Bile powder by screening through a 100 mesh sieve after crushing;

[0024] (2) decocting Coptis chinensis, Gentiana scabra and Lily according to a weight ratio of 3:3:1, and filtering to obtain medicinal liquid; wherein the total weight of the Coptis chinensis, the Gentiana scabra and the Lily is 1:35 by weight of the water, and the decocting time is 30 minutes.

[0025] (3) stirring the Arisaema cum Bile powder, the bile and the medicinal liquid uniformly according to a weight ratio of 1:2.5:1;

[0026] (4) placing the mixture of step (3) in a fermentation barrel for fermentation, wherein the fermentation time is 7 days, and the fermentation barrel is kept at a constant temperature of 30℃ for fermentation.

[0027] (5) The fermented Danna Xing is dried or baked into shape and then sliced. Example 2

[0028] The difference between this example and Example 1 is:

[0029] Step (1), the Arisaema consanguineum is crushed and then 80-mesh sieved to obtain fine-particle Arisaema consanguineum powder;

[0030] Step (2), the total weight of Coptis chinensis, Gentiana scabra and lily is 1:50 of the weight of water, and the decocting time is 60 minutes.

[0031] Step (3), the Arisaema consanguineum powder, bile and medicinal liquid are stirred uniformly according to a weight ratio of 1:3:1;

[0032] Step (4) the fermentation time is 9 days, and the fermentation barrel is kept at a constant temperature of 38℃ for fermentation. Example 3

[0033] The difference between this example and Example 1 is:

[0034] Step (1), the Arisaema consanguineum is crushed and then 90-mesh sieved to obtain fine-particle Arisaema consanguineum powder;

[0035] Step (2), the total weight of Coptis chinensis, Gentiana scabra and lily is 1:50 of the weight of water, and the decocting time is 60 minutes.

[0036] Step (3), the Arisaema consanguineum powder, bile and medicinal liquid are stirred uniformly according to a weight ratio of 1:3:1;

[0037] Step (4) the fermentation time is 9 days, and the fermentation barrel is kept at a constant temperature of 38℃ for fermentation.

[0038] 1. 300g of Danna Xing prepared in Example 1, 2 and 3 of the present application are taken as Sample No. 1, Sample No. 2 and Sample No. 3 respectively;

[0039] 2. Danna Xing is prepared according to the following method, and 300g of the prepared Danna Xing is taken as Sample No. 4:

[0040] (1) Arisaema consanguineum is crushed into powder; the Arisaema consanguineum powder is 100-mesh sieved to obtain fine-particle Arisaema consanguineum powder;

[0041] (2) the Arisaema consanguineum powder and bile are stirred uniformly according to a weight ratio of 1:2.5;

[0042] (3) the mixture of step (2) is placed in a fermentation barrel for fermentation, the fermentation time is 7 days, and the fermentation barrel is kept at a constant temperature of 30℃ for fermentation;

[0043] (4) the fermented Danna Xing is dried or baked into shape and then sliced.

[0044] 3. Prepare the Arisaema cum Bile according to the following method, and take 300g of the prepared Arisaema cum Bile as Sample No. 5:

[0045] (1) Crush the Arisaema into powder; and take the fine-particle Arisaema powder obtained after 80-mesh screening;

[0046] (2) Stir the Arisaema powder and the bile uniformly according to the weight ratio of 1:2;

[0047] (3) Place the mixture of step (2) in a fermentation vat for fermentation, and the fermentation time is 5 days, and the fermentation vat is kept at a constant temperature of 28°C for fermentation;

[0048] (4) Dry the fermented Arisaema cum Bile, and slice the dried Arisaema cum Bile.

[0049] 4. Prepare the Arisaema cum Bile according to the following method, and take 300g of the prepared Arisaema cum Bile as Sample No. 6:

[0050] (1) Crush the Arisaema into powder; and take the fine-particle Arisaema powder obtained after 90-mesh screening;

[0051] (2) Stir the Arisaema powder and the bile uniformly according to the weight ratio of 1:3;

[0052] (3) The fermentation time is 9 days, and the fermentation vat is kept at a constant temperature of 38°C for fermentation.

[0053] (4) Dry the fermented Arisaema cum Bile, and slice the dried Arisaema cum Bile.

[0054] 5. Prepare the Arisaema cum Bile according to the following method, and take 300g of the prepared Arisaema cum Bile as Sample No. 7:

[0055] (1) Crush the Arisaema into powder; and take the fine-particle Arisaema powder obtained after 100-mesh screening;

[0056] (2) Stir the Arisaema powder and the bile uniformly according to the weight ratio of 1:4;

[0057] (3) Place the mixture of step (2) in a fermentation vat for fermentation, and the fermentation time is 7 days, and the fermentation vat is kept at a constant temperature of 30°C for fermentation;

[0058] (4) Dry the fermented Arisaema cum Bile, and slice the dried Arisaema cum Bile.

[0059] 6. Prepare the Arisaema cum Bile according to the following method, and take 300g of the prepared Arisaema cum Bile as Sample No. 8:

[0060] (1) Crush the Arisaema into powder; and take the fine-particle Arisaema powder obtained after 100-mesh screening;

[0061] (2) Stir the Arisaema powder and the bile uniformly according to the weight ratio of 1:5;

[0062] (3) The mixture of step (2) is placed in a fermentation barrel for fermentation, and the fermentation time is 7 days, and the fermentation barrel is kept at a constant temperature of 30 DEG C for fermentation;

[0063] (4) The dried or dried and shaped arisaema cum bile is sliced after fermentation.

[0064] The above-mentioned 8 kinds of samples are respectively added with 6 times of 80 DEG C water for extraction for 3 times, 1 hour each time, and the filtrates are combined and concentrated to 0.14 g / mL.

[0065] 90 14-day-old male rats (50 ± 5 g) are raised at a temperature of 22-25 DEG C, a relative humidity of 55% ± 10% in the feeding environment, and light and dark alternation every 12 hours, and after adaptive feeding for 7 days, they are divided into 9 groups, 10 rats in each group, namely, the blank group, the 1st group, the 2nd group, the 3rd group, the 4th group, the 5th group, the 6th group, the 7th group and the 8th group. The hot water bath method (45 ± 2 DEG C) is used to establish the febrile convulsion model, and the blank group is placed in a water bath at 37 DEG C, and the other groups of rats are excited every 1 day for 7 times. After modeling for 2 hours, the 1st to 8th groups of rats are respectively given 10 mL / kg of the liquid medicine prepared by the 1st to 8th samples by gavage, and the blank group is given the same volume of normal saline, for a total of 7 days.

[0066] After the last administration and modeling, the convulsion latency, convulsion duration, convulsion level and mortality rate of each group of rats are observed. The convulsion latency refers to the time from the rat being put into the hot water to the occurrence of convulsion, and the recording unit is second (s). The convulsion time refers to the time from the occurrence of convulsion of the rat to the stop, and the recording unit is second (s). The convulsion level is as follows: 0 level, no convulsion; 1 level, appearance of nodding, blinking and other facial convulsion behaviors; 2 level, nodding and whisker movement with rhythm; 3 level, forelimb clonic convulsion; 4 level, forelimb clonic convulsion and general rigidity; 5 level, general rigidity and fall.

[0067]

[0068] It can be known from the comparison between the 1st group and the 4th group that the arisaema cum bile prepared by arisaema powder and bile according to a weight ratio of 1:2.5 has better effect after the addition of the liquid medicine prepared by the present application, prolongs the convulsion latency, shortens the convulsion time, and has lower convulsion level.

[0069] It can be known from the comparison between the 1st group and the 7th group that the arisaema powder and bile in the 1st group are in a ratio of 1:2.5, and the arisaema powder and bile in the 7th group are in a ratio of 1:4. After the addition of the liquid medicine prepared by the present application, the prevention and treatment of febrile convulsion of the present application are slightly better than those of the 7th group, prolong the convulsion latency, and shorten the convulsion time.

[0070] Comparing group 1 and group 8, the proportion of arisaema erubescens powder and bile in group 1 is 1:2.5, and the proportion of arisaema erubescens powder and bile in group 7 is 1:5, and the effect of the present application on the prevention and treatment of heat convulsion is almost the same as that of group 7 after adding the compatible medicinal liquid.

[0071] Comparing group 2 and group 5, arisaema erubescens powder and bile are prepared according to the weight ratio of 1:2, and the effect is better after adding the compatible medicinal liquid of the present application, the latent period of convulsion is prolonged, the convulsion time is shortened, and the convulsion grade is lower, in addition, the effect of group 5 is not good in treating heat convulsion in the experiment due to the small amount of bile used, while the present application still has good effect.

[0072] Comparing group 3 and group 6, arisaema erubescens powder and bile are prepared according to the weight ratio of 1:3, and the effect is better after adding the compatible medicinal liquid of the present application, the latent period of convulsion is prolonged, the convulsion time is shortened, and the convulsion grade is lower, in addition, the effect of group 6 is not ideal in treating heat convulsion in the experiment due to the low amount of bile used, while the present application still has good effect.

[0073] From the above control test, it can be known that the present application increases the filter liquid of coptis chinensis, gentiana scabra and lily by the ratio, utilizes the mutual action and the synergistic effect of drugs in the body, and compared with the prior art, the amount of bile used is less under the condition of ensuring the drug effect of arisaema erubescens unchanged or better, which has significant progress.

[0074] Finally, it should be noted that the above examples are only used to illustrate the technical solutions of the present application and not to limit the protection scope of the claims. Those skilled in the art should understand that the technical solutions of the present application can be modified or replaced by equivalents, but belong to the same and the protection scope of the present application.

Claims

1. A method for preparing Arisaema cum Bile, characterized in that, It includes the following main steps: (1) Crush Arisaema into powder; (2) Boil Coptis chinensis, Gentiana scabra and Lilium brownii in water in a weight ratio of 3:3:1, filter and take the decoction. (3) Mix the Arisaema powder, bile and medicinal liquid in a weight ratio of 1:2 to 3:1 until homogeneous; (4) Place the mixture from step (3) into a fermentation tank for fermentation; (5) After fermentation, Arisaema cum Bile is dried or baked and then sliced.

2. The method for preparing Arisaema cum Bile according to claim 1, characterized in that, Step (1) After crushing Arisaema, sieve it through an 80-100 mesh screen to obtain fine Arisaema powder.

3. The method for preparing Arisaema cum Bile according to claim 1, characterized in that, In step (2), the total weight of Coptis chinensis, Gentiana scabra and Lilium brownii is in a weight ratio of 1:25~50 to water.

4. The method for preparing Arisaema cum Bile according to claim 1, characterized in that, Step (2), the simmering time is 25~60 minutes.

5. The method for preparing Arisaema cum Bile according to claim 4, characterized in that, Step (2), the simmering time is 30 minutes.

6. The method for preparing Arisaema cum Bile according to claim 1, characterized in that, Step (4) fermentation time is 5 to 9 days.

7. The method for preparing Arisaema cum Bile according to claim 6, characterized in that, Step (4) fermentation time is 7 days.

8. The method for preparing Arisaema cum Bile according to claim 1, characterized in that, Step (4) Ferment at a constant temperature of 28~38℃ in the fermentation tank.

9. The method for preparing Arisaema cum Bile according to claim 8, characterized in that, Step (4) Ferment at a constant temperature of 30℃ in the fermentation tank.

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