A pesticide composition for preventing and treating asparagus root rot and a preparation method and application thereof

Abstract

The present application belongs to the technical field of root rot prevention, and relates to a pesticide composition for preventing and treating asparagus root rot, and a preparation method and application thereof. The pesticide composition for preventing and treating asparagus root rot is prepared from the following raw materials: 5-10% of artemisia extract, 5-10% of marigold extract, 5-10% of azoxystrobin, 10-20% of difenoconazole, 5-10% of copper sulfate, 5-10% of benzoic acid, 1-2% of plant growth regulator, 1-5% of non-ionic surfactant, and the balance of water. The pesticide composition contains plant extracts and fungicides with different mechanisms, and the combination of the two achieves excellent efficacy in preventing and treating root rot. In addition, all components in the pesticide composition are low-toxic or micro-toxic substances, and are relatively safe to the environment and human bodies, and do not cause great harm.

Description

Technical Field

[0001] This invention belongs to the field of root rot control technology, and more specifically relates to a pesticide composition for controlling asparagus root rot, its preparation method and application. Background Technology

[0002] Asparagus is a nutritious vegetable, and root rot severely affects its yield and quality. Currently, pesticides used to control asparagus root rot mainly include organophosphates, carbamates, and benzoylurea. However, these pesticides have certain drawbacks in their use, such as environmental pollution, harm to human health, and impact on asparagus quality. Therefore, developing a safe and efficient pesticide composition for controlling asparagus root rot is an urgent problem to be solved. Summary of the Invention

[0003] The purpose of this invention is to provide a pesticide composition for preventing and controlling asparagus root rot, its preparation method, and its application, so as to solve the problems existing in the prior art and achieve safe and efficient prevention and control of asparagus root rot.

[0004] To achieve the above objectives, the present invention provides the following solution:

[0005] One of the technical solutions of this invention: provides a pesticide composition for preventing and controlling asparagus root rot, wherein the raw materials are, by mass percentage:

[0006] The ingredients include 5%–10% Artemisia argyi extract, 5%–10% Marigold extract, 5%–10% azoxystrobin, 10%–20% difenoconazole, 5%–10% copper sulfate, 5%–10% benzoic acid, 1%–2% plant growth regulator, 1%–5% nonionic surfactant, and the remainder is water.

[0007] Furthermore, the plant growth regulator is a cytokinin (CTK) or a gibberellin (GA).

[0008] Furthermore, the nonionic surfactant is Tween 80, NP-10, or emulsifier EL-40.

[0009] The pesticide composition of this invention includes plant extracts and fungicides with different mechanisms of action. Among them, Artemisia argyi extract and marigold extract, as natural plant extracts, have antibacterial, antiviral, and antioxidant effects, which can enhance the disease resistance of plants. Generally, plant extracts with antibacterial effects will lose their antibacterial effect after dilution due to excessive dilution. However, the Artemisia argyi extract and marigold extract selected in this invention still have excellent antibacterial effects after dilution, and work synergistically with fungicides to prevent and control root rot.

[0010] The second technical solution of the present invention provides a method for preparing the above-mentioned pesticide composition for controlling asparagus root rot, comprising the following steps:

[0011] A mixture of azoxystrobin, difenoconazole and copper sulfate was added to half the volume of water and then ball-milled to disperse the mixture, thus obtaining solution A.

[0012] After mixing the Artemisia argyi extract, marigold extract, benzoic acid and plant growth regulator, add the remaining water and stir until well mixed to obtain solution B.

[0013] After mixing liquid A and liquid B evenly, a nonionic surfactant is added, and the mixture is homogenized and dispersed to obtain the pesticide composition.

[0014] Furthermore, the ball milling dispersion time is 30–60 min.

[0015] Furthermore, the homogenization dispersion time is 20–30 min.

[0016] The third technical solution of the present invention provides an application of the above-mentioned pesticide composition in the prevention and control of asparagus root rot.

[0017] Furthermore, the application method is spraying or root irrigation.

[0018] Preferably, the spraying is performed after dilution by 500 to 1000 times.

[0019] Preferably, the root irrigation is performed after dilution by 50 to 100 times.

[0020] The present invention discloses the following technical effects:

[0021] This invention provides a pesticide composition prepared by mixing various fungicides with different mechanisms of action, such as difenoconazole, azoxystrobin, and copper sulfate, with plant extracts, which can effectively improve the control effect.

[0022] The pesticide composition of the present invention contains only low-toxicity or slightly toxic substances, which are relatively safe for the environment and human body and will not cause significant harm.

[0023] All components in the pesticide composition of the present invention are biodegradable substances, which will not cause significant pollution to soil and water sources, and meet the requirements of green environmental protection.

[0024] The pesticide composition of the present invention incorporates plant growth regulators, which can promote the growth of asparagus, improve the disease resistance of asparagus, and further enhance the control effect. Detailed Implementation

[0025] Various exemplary embodiments of the present invention will now be described in detail. This detailed description should not be considered as a limitation of the present invention, but rather as a more detailed description of certain aspects, features, and embodiments of the present invention.

[0026] It should be understood that the terminology used in this invention is merely for describing particular embodiments and is not intended to limit the invention. Furthermore, with respect to numerical ranges in this invention, it should be understood that each intermediate value between the upper and lower limits of the range is also specifically disclosed. Any stated value or intermediate value within a stated range, as well as each smaller range between any other stated value or intermediate value within said range, is also included in this invention. The upper and lower limits of these smaller ranges may be independently included or excluded from the range.

[0027] Unless otherwise stated, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art. While only preferred methods and materials have been described herein, any methods and materials similar or equivalent to those described herein may be used in the implementation or testing of this invention. All references to this specification are incorporated by way of citation to disclose and describe methods and / or materials associated with those references. In the event of any conflict with any incorporated reference, the content of this specification shall prevail.

[0028] Various modifications and variations can be made to the specific embodiments described in this specification without departing from the scope or spirit of the invention, as will be apparent to those skilled in the art. Other embodiments derived from this specification will also be readily apparent to those skilled in the art. This specification and embodiments are merely exemplary.

[0029] The terms “include,” “including,” “have,” “contain,” etc., used in this article are all open-ended terms, meaning that they include but are not limited to.

[0030] The Artemisia argyi extract and Marigold extract used in the specific embodiments of the present invention were provided by Xi'an An'ao Biotechnology Co., Ltd., and both were obtained by alcohol extraction with a material-to-liquid ratio of 1:10.

[0031] Example 1

[0032] The preparation steps for the pesticide composition for controlling asparagus root rot are as follows:

[0033] The raw materials, by weight percentage, are: 10% Artemisia argyi extract, 10% Marigold extract, 10% azoxystrobin, 20% difenoconazole, 10% copper sulfate, 10% benzoic acid, 1% plant growth regulator (cytokinin (CTK)) and 5% nonionic surfactant (Tween 80), with the remainder being water.

[0034] The preparation steps are as follows: Azoxystrobin, difenoconazole and copper sulfate are mixed and then 1 / 2 volume of water is added for ball milling dispersion (60 min) to obtain solution A; Artemisia argyi extract, marigold extract, benzoic acid and plant growth regulator are mixed and then the remaining water is added and stirred to obtain solution B; solution A and solution B are mixed evenly, and then a nonionic surfactant is added and homogenized and dispersed (30 min) to obtain the pesticide composition.

[0035] Example 2

[0036] The preparation steps for the pesticide composition for controlling asparagus root rot are as follows:

[0037] The raw materials, by mass percentage, are: 5% Artemisia argyi extract, 10% Marigold extract, 5% azoxystrobin, 20% difenoconazole, 10% copper sulfate, 5% benzoic acid, 1.5% plant growth regulator (gibberellin (GA)) and 3% nonionic surfactant (NP-10), with the remainder being water.

[0038] The preparation steps are as follows: Azoxystrobin, difenoconazole and copper sulfate are mixed and then 1 / 2 volume of water is added for ball milling dispersion (50 min) to obtain solution A; Artemisia argyi extract, marigold extract, benzoic acid and plant growth regulator are mixed and then the remaining water is added and stirred to obtain solution B; solution A and solution B are mixed evenly, and then a nonionic surfactant is added and homogenized and dispersed (25 min) to obtain the pesticide composition.

[0039] Example 3

[0040] The preparation steps for the pesticide composition for controlling asparagus root rot are as follows:

[0041] The raw materials, by mass percentage, are: 8% Artemisia argyi extract, 8% Marigold extract, 8% azoxystrobin, 15% difenoconazole, 8% copper sulfate, 8% benzoic acid, 2% plant growth regulator (gibberellin (GA)) and 1% nonionic surfactant (emulsifier EL-40), with the remainder being water.

[0042] The preparation steps are as follows: Azoxystrobin, difenoconazole and copper sulfate are mixed and then 1 / 2 of the amount of water is added for ball milling dispersion (30 min) to obtain solution A; Artemisia argyi extract, marigold extract, benzoic acid and plant growth regulator are mixed and then the remaining water is added and stirred to obtain solution B; solution A and solution B are mixed evenly, and then a nonionic surfactant is added and homogenized and dispersed (20 min) to obtain the pesticide composition.

[0043] Comparative Example 1

[0044] The preparation steps for the pesticide composition for controlling asparagus root rot are as follows:

[0045] The raw materials, by mass percentage, are: marigold extract 10%, azoxystrobin 10%, difenoconazole 20%, copper sulfate 10%, benzoic acid 10%, plant growth regulator (cytokinin (CTK)) 1%, and nonionic surfactant (Tween 80) 5%, with the remainder being water.

[0046] The preparation steps are as follows: Azoxystrobin, difenoconazole and copper sulfate are mixed and then 1 / 2 volume of water is added for ball milling dispersion (60 min) to obtain solution A; marigold extract, benzoic acid and plant growth regulator are mixed and then the remaining water is added and stirred to obtain solution B; solution A and solution B are mixed evenly, and then a nonionic surfactant is added and homogenized and dispersed (30 min) to obtain the pesticide composition.

[0047] Comparative Example 2

[0048] The preparation steps for the pesticide composition for controlling asparagus root rot are as follows:

[0049] The raw materials, by mass percentage, are: 10% Artemisia argyi extract, 10% azoxystrobin, 20% difenoconazole, 10% copper sulfate, 10% benzoic acid, 1% plant growth regulator (cytokinin (CTK)) and 5% nonionic surfactant (Tween 80), with the remainder being water.

[0050] The preparation steps are as follows: Azoxystrobin, difenoconazole and copper sulfate are mixed and then 1 / 2 of the amount of water is added for ball milling dispersion (60 min) to obtain solution A; Artemisia argyi extract, benzoic acid and plant growth regulator are mixed and then the remaining water is added and stirred to obtain solution B; solution A and solution B are mixed evenly, and then a nonionic surfactant is added and homogenized and dispersed (30 min) to obtain the pesticide composition.

[0051] Comparative Example 3

[0052] The preparation steps for the pesticide composition for controlling asparagus root rot are as follows:

[0053] The raw materials, by mass percentage, are: 10% azoxystrobin, 20% difenoconazole, 5%–10% copper sulfate, 10% benzoic acid, 1% plant growth regulator (cytokinin (CTK)) and 5% nonionic surfactant (Tween 80), with the remainder being water.

[0054] The preparation steps are as follows: Azoxystrobin, difenoconazole and copper sulfate are mixed and then 1 / 2 of the amount of water is added for ball milling dispersion (60 min) to obtain solution A; benzoic acid and plant growth regulator are mixed and then the remaining water is added and stirred to obtain solution B; solution A and solution B are mixed evenly, and then a nonionic surfactant is added and homogenized and dispersed (30 min) to obtain the pesticide composition.

[0055] Comparative Example 4

[0056] The preparation steps for the pesticide composition for controlling asparagus root rot are as follows:

[0057] The raw materials, by mass percentage, are: 10% Artemisia argyi extract, 10% Marigold extract, 10% Benzoic acid, 1% Plant growth regulator (cytokinin (CTK)) and 5% Nonionic surfactant (Tween 80), with the remainder being water.

[0058] The preparation steps are as follows: Artemisia argyi extract, marigold extract, benzoic acid and plant growth regulator are mixed and then added to water and stirred until evenly mixed. Then, a nonionic surfactant is added and the mixture is homogenized and dispersed (30 min) to obtain the pesticide composition.

[0059] Test case

[0060] A sandy loam experimental field was selected and divided into seven non-interfering treatment groups. The pesticide compositions prepared in Examples 1-3 and Comparative Examples 1-4 were applied to each group, respectively. A blank control group (CK) without pesticide application was also included. Each treatment was replicated three times, and each replicate covered an area of ​​10 m². 2 Each plant was repeated with 50 asparagus plants, and the same field management practices were adopted.

[0061] The specific method for applying the pesticide composition to the above treatment group was as follows: 30 days after planting, the pesticide composition was sprayed. Specifically, the pesticide composition was diluted 1000 times, and the amount of each repeated spray was 4L. On the 30th and 60th days after the pesticide was applied, the growth of asparagus was observed, the occurrence of root rot was recorded, and the incidence rate was calculated. The results are shown in Table 1.

[0062] Incidence rate = Number of infected plants / Number of planted plants

[0063] Table 1

[0064] CK Example 1 Example 2 Example 3 Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 30d 11.3％ 0.0％ 0.7％ 0.0％ 2.0％ 2.7％ 4.0％ 7.3％ 60d 25.3％ 0.7％ 3.3％ 1.3％ 7.3％ 6.0％ 9.3％ 19.3％

[0065] Table 1 shows the incidence rate of root rot in asparagus 30 to 60 days after spraying. The comparison shows that the pesticide compositions prepared in Examples 1-3 of this invention have good control effects on root rot, with the incidence rate remaining below 4% even after 60 days. Specifically, the incidence rates of Comparative Examples 1 and 2 were 6%–7.3% after 60 days, Comparative Example 3 was 9.3%, and Comparative Example 4 was 19.3%. Compared with the blank control group, it can be seen that the combined use of the plant extract and fungicide in this invention has a synergistic effect in controlling root rot.

[0066] This experimental example uses spraying to apply the pesticide composition. In practical applications, if the root rot disease is severe, root irrigation can be selected. The pesticide composition prepared in this invention can achieve excellent control of root rot disease through both spraying and root irrigation.

[0067] The various embodiments in this specification are described in a progressive manner, with each embodiment focusing on the differences from other embodiments. The same or similar parts between the various embodiments can be referred to each other.

[0068] The above description of the disclosed embodiments enables those skilled in the art to make or use the invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the invention. Therefore, the invention is not to be limited to the embodiments shown herein, but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A pesticide composition for controlling asparagus root rot, characterized in that, By weight percentage, the raw materials are: Artemisia argyi extract 5%~10%, marigold extract 5%~10%, azoxystrobin 5%~10%, difenoconazole 10%~20%, copper sulfate 5%~10%, benzoic acid 5%~10%, plant growth regulator 1%~2%, nonionic surfactant 1%~5%, balance is water; The Artemisia argyi extract and marigold extract are extracts prepared by alcohol extraction with a material-to-liquid ratio of 1:

10. The preparation steps of the pesticide composition for controlling asparagus root rot include: A mixture of azoxystrobin, difenoconazole and copper sulfate was added to half the volume of water and then ball-milled to disperse the mixture, thus obtaining solution A. After mixing the Artemisia argyi extract, marigold extract, benzoic acid and plant growth regulator, add the remaining water and stir until well mixed to obtain solution B. After mixing liquid A and liquid B evenly, a nonionic surfactant is added, and the mixture is homogenized and dispersed to obtain the pesticide composition. The plant growth regulators mentioned are cytokinins or gibberellins.

2. The pesticide composition according to claim 1, characterized in that, The nonionic surfactant is Tween 80, NP-10, or emulsifier EL-40.

3. A method for preparing a pesticide composition for controlling asparagus root rot as described in any one of claims 1 to 2, characterized in that the step... include: A mixture of azoxystrobin, difenoconazole and copper sulfate was added to half the volume of water and then ball-milled to disperse the mixture, thus obtaining solution A. After mixing the Artemisia argyi extract, marigold extract, benzoic acid and plant growth regulator, add the remaining water and stir until well mixed to obtain solution B. After mixing liquid A and liquid B evenly, a nonionic surfactant is added, and the mixture is homogenized and dispersed to obtain the pesticide composition.

4. The preparation method according to claim 3, characterized in that, The ball milling dispersion time is 30~60 min.

5. The preparation method according to claim 3, characterized in that, The homogenization dispersion time is 20-30 min.

6. The use of a pesticide composition as described in any one of claims 1 to 2 in the control of asparagus root rot.

7. The application according to claim 6, characterized in that, The application method is spraying or root irrigation.

8. The application according to claim 7, characterized in that, The spraying is performed after dilution of 500 to 1000 times.

9. The application according to claim 7, characterized in that, The root irrigation is performed after dilution by 50 to 100 times.