A pair of antigen-binding proteins that specifically bind to Bacillus cereus Nhe A
By developing recombinant antigen-binding proteins 5C1 and 8G7 that specifically bind to Bacillus cereus NheA, the stability and consistency issues of traditional monoclonal antibodies in Bacillus cereus detection have been resolved, achieving high affinity and specificity for NheA detection, reducing storage costs and improving detection reliability.
CN118459579BActive Publication Date: 2026-06-05JIANGNAN UNIV
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- JIANGNAN UNIV
- Filing Date
- 2024-04-30
- Publication Date
- 2026-06-05
Smart Images
Figure CN118459579B_ABST
Abstract
The application relates to a pair of antigen binding proteins specifically combined with Bacillus cereus NheA, and relates to the field of biological immunization technology. The application obtains a pair of antigen binding proteins specifically combined with Bacillus cereus NheA through screening, and the pair of antigen binding proteins are named as 5C1 and 8G7 and have unique variable region sequences. The non-hemolytic enterotoxin Nhe A of Bacillus cereus is detected by using a double-antibody sandwich detection method with 5C1 as a capture antibody and 8G7 as a detection antibody, and the minimum detection limit is 0.8 ng / mL, which indicates that the recombinant antibody has good sensitivity to Nhe A. In addition, the recombinant antibody has no cross reaction to other microbial toxins and has good specificity.
Need to check novelty before this filing date? Find Prior Art