A full-information thin-layer quality evaluation method for Sichuan and Lu Codonopsis pilosula with different origins and places of origin
By comparing the component information spots of Codonopsis pilosula and Codonopsis pilosula in polar gradient areas through thin-layer identification method, the problem of quality evaluation of medicinal materials in multiple basic sources and multiple production areas was solved, and a simple and fast multi-region full information quality evaluation method was provided. The internal quality of the two is similar, and the difference in the base source and origin does not affect the product quality.
Patent Information
- Application Number
- CN202410572101.X
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2024-05-10
- Publication Date
- 2024-12-10
- Estimated Expiration
- 2044-05-10
AI Technical Summary
The prior art lacks simple, fast and efficient methods to distinguish and evaluate the quality of Codonopsis medicinal materials from multiple sources and multiple sources, especially to determine their base and origin in compound preparations.
The thin-layer identification method was used to compare the component information spots of Codonopsis pilosula and Codonopsis pilosula in 6 polar gradient areas. Through the combination of different components and polar expanders, methanol ultrasonic treatment and ethanol sulfate color development technology were used to separate and inspect. The ingredients of medicinal materials provide multi-region full information quality evaluation methods.
The internal quality evaluation of Sichuan Codonopsis and Lu Codonopsis was realized. It was found that the two were basically the same in terms of active ingredients, and the base source and origin did not affect the product quality. It provided a basis for selection and a simple multi-region thin layer identification method, which improved the Detect information volume and efficiency.
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Figure CN118465167B_ABST
Abstract
Description
Technical Field
[0001] The present invention relates to a method for evaluating the quality of Sichuan Codonopsis and Lu Codonopsis with full information of different origins and places of origin. That is, by using a thin layer identification method, the information spots of ten components of Sichuan Codonopsis and Lu Codonopsis are compared in the fat-soluble, medium-polar and water-soluble regions, and the difference components of the two Codonopsis are found to achieve the purpose of correctly evaluating the internal quality of the two Codonopsis. Background Art
[0002] Codonopsis pilosula is the dried root of Codonopsis pilosula (Franch. Nannf., Codonopsis pilosula Nannf. var. modesta (Nannf.) LTShen or Codonopsis tangshen Oliv of the Campanulaceae family. It is harvested in autumn, washed and dried in the sun. What are the differences in quality between Lu Codonopsis pilosula and Chuan Codonopsis tangshen, which have different sources and origins? There are no relevant reports so far. In particular, in order to ensure product quality, it is required that the sources and origins of multi-source and multi-origin medicinal materials in compound preparations must be determined. It is urgent to explore a simple, fast, efficient and informative full-information quality evaluation method for multi-source and multi-origin medicinal materials.
[0003] In order to ensure the intrinsic quality of the compound preparation of Codonopsis pilosula and provide a simple, fast, efficient and easy-to-popularize thin-layer quality evaluation method, a special thin-layer identification study was conducted on the fat-soluble, medium-polar and polar components of the two medicinal materials using developing agents of different components and polarities. An evaluation method for examining more than 70 component information spots in 6 polarity gradient regions and multiple inspection conditions was obtained. Summary of the invention
[0004] The supernatant of methanol ultrasonic was used as the test solution of the two medicinal materials. In 6 polarity gradient areas and under various inspection conditions, a total of 74 spots of component information were detected for Sichuan Codonopsis and 73 for Lu Codonopsis. Sichuan Codonopsis only had 2 more spots of components with very low content in the detection area (1) and 1 less fluorescent spot with poor stability in the detection area (4) than Lu Codonopsis. The similarity of the components of Codonopsis from the two origins was 97.3%. Although Sichuan Codonopsis and Lu Codonopsis had different origins and origins, they contained basically the same effective ingredients and the same internal quality, which did not affect the quality of the raw materials. This provides a selection basis and a multi-region thin-layer identification exclusive method for compound preparations containing Codonopsis.
[0005] The solution adopted by the present invention to solve the technical problem is:
[0006] (1) Comparison of the strong fat-soluble components of Sichuan Codonopsis and Lu Codonopsis medicinal materials. Take 0.1 g of Sichuan Codonopsis and Lu Codonopsis medicinal materials, add 2 ml of methanol, and treat them ultrasonically for 20 minutes. The supernatant is used as the test solution for each. Take 5-6 μl of each of the above two solutions and spot them on the same silica gel GF 254 On the thin layer plate, cyclohexane-ethyl acetate-formic acid with a volume ratio of 10:1:0.1 was used as the developing agent, developed, taken out, dried with hot air, sprayed with 10% sulfuric acid ethanol solution, heated at 105°C until the spots were clearly colored, and examined under a 365nm ultraviolet lamp. Sichuan Codonopsis showed 9 fluorescent spots of different colors; Lu Codonopsis showed 8 fluorescent spots of different colors. The only difference between the two Codonopsis was that Sichuan Codonopsis had one more fluorescent spot than Lu Codonopsis, and the remaining 8 fluorescent spots were completely consistent. The thin layer plate was examined under sunlight again. Sichuan Codonopsis showed 8 color spots; Lu Codonopsis showed 7 color spots. The difference between the two origins of Codonopsis was that Sichuan Codonopsis had one more color spot than Lu Codonopsis, and the remaining 7 color spots were completely consistent.
[0007] (2) Thin layer contrast analysis of fat-soluble components of Sichuan Codonopsis pilosula and Lu Codonopsis pilosula. 5 μl of the test solution under (1) was applied to the same silica gel GF 254 On the thin layer plate, cyclohexane-ethyl acetate-formic acid with a volume ratio of 8:1.5:0.1 was used as the developing agent, developed, taken out, dried with hot air, sprayed with 10% sulfuric acid ethanol solution, heated at 105℃ until the spots were clearly colored, and examined under a 365nm ultraviolet lamp. Both Sichuan Codonopsis and Lu Codonopsis showed 8 completely identical fluorescent spots, the only difference was that the fluorescence intensity of some spots of Lu Codonopsis was slightly weaker than that of Sichuan Codonopsis; the thin layer plate was examined under sunlight again, and both Sichuan Codonopsis and Lu Codonopsis showed 7 completely identical color spots, the only difference was that some color spots of Lu Codonopsis were slightly lighter than those of Sichuan Codonopsis;
[0008] (3) Thin layer contrast analysis of fat-soluble components of Sichuan Codonopsis pilosula and Lu Codonopsis pilosula. 5-6 μl of the test solution under (1) were applied to the same silica gel GF 254 On the thin layer plate, cyclohexane-ethyl acetate-formic acid with a volume ratio of 7:3:0.1 was used as the developing agent, developed, taken out, dried with hot air, sprayed with 10% sulfuric acid ethanol solution, heated at 105℃ until the spots were clearly colored, and examined under a 365nm ultraviolet lamp. Both Sichuan Codonopsis and Lu Codonopsis showed 5 clear and identical main fluorescent spots; the thin layer plate was examined under sunlight again, and both Sichuan Codonopsis and Lu Codonopsis showed 7 completely identical color spots; under the two inspection conditions, the spots of the two Codonopsis components were basically the same;
[0009] (4) Thin layer contrast analysis of the polar components of Sichuan Codonopsis pilosula and Lu Codonopsis pilosula. 5-6 μl of the test solution under (1) were applied to the same silica gel GF 254On the thin layer plate, cyclohexane-ethyl acetate-formic acid with a volume ratio of 3:7:0.1 was used as the developing agent, developed, taken out, dried with hot air, sprayed with 10% sulfuric acid ethanol solution, heated at 105°C until the spots were clearly colored, and examined under a 365nm ultraviolet lamp. Codonopsis pilosula showed 6 fluorescent spots of different colors; Codonopsis pilosula in Lu showed 7 fluorescent spots of different colors. The difference between the two Codonopsis pilosula was that Codonopsis pilosula in Lu had one more blue-purple fluorescent spot than Codonopsis pilosula in Sichuan, and the other 6 fluorescent spots were basically the same for the two Codonopsis pilosula. The thin layer plate was examined under sunlight again, and both Codonopsis pilosula in Sichuan and Codonopsis pilosula in Lu showed 7 completely identical color spots.
[0010] (5) Thin layer contrast analysis of water-soluble components of Sichuan Codonopsis pilosula and Lu Codonopsis pilosula. 5-6 μl of the test solution under (1) were applied to the same silica gel GF 254 On the thin layer plate, chloroform-ethyl acetate-methanol-concentrated ammonia solution with a volume ratio of 2:5:3:0.5 was used as the developing agent, developed, taken out, blown dry with hot air, sprayed with 10% sulfuric acid ethanol solution, heated at 105℃ until the spots were clearly colored, and examined under ultraviolet light at 365nm. Both Sichuan Codonopsis and Lu Codonopsis showed 4 clear and identical main fluorescent spots; the thin layer plate was examined under sunlight again, and both Sichuan Codonopsis and Lu Codonopsis showed 5 completely identical color spots; under the two inspection conditions, the spots of the two Codonopsis components were basically the same;
[0011] (6) Thin layer contrast analysis of water-soluble components of Sichuan Codonopsis pilosula and Lu Codonopsis pilosula. 5-6 μl of the test solution under (1) were applied to the same silica gel GF 254 On the thin layer plate, chloroform-ethyl acetate-methanol-concentrated ammonia test solution with a volume ratio of 1.5:4:4:0.8 was used as the developing agent. The plate was taken out, dried with hot air, sprayed with 10% sulfuric acid ethanol solution, heated at 105°C until the spots were clearly colored, and examined under ultraviolet light at 365nm. Both Sichuan Codonopsis and Lu Codonopsis showed 3 clear identical fluorescent main spots. The thin layer plate was examined under sunlight again. Both Sichuan Codonopsis and Lu Codonopsis showed 5 completely identical color spots. Under the two inspection conditions, the spots of the two Codonopsis components were basically the same.
[0012] According to the chemical structure and properties of the active ingredients reported by Codonopsis pilosula, the principle of similar and compatible extraction is followed, and suitable extraction solvents and the same extraction method are used to prepare the test solution simply and quickly. Then, using developing agents of different components and polarities for development, various chemical components will be separated on their respective thin layer plates according to the different adsorption, desorption, re-adsorption, and re-desorption abilities of different developing agents. Then, the active ingredients developed by a variety of developing agents are made to present different color spots with the help of color developers and different inspection conditions. Then, according to the separation of the exclusive spots, by continuously adjusting the developing agent components and ratios, the exclusive spots in different regions can be clearly distinguished, providing an intrinsic quality evaluation method for the full information of multiple regions of Sichuan Codonopsis pilosula and Lu Codonopsis pilosula.
[0013] The innovative features and beneficial effects of the present invention are as follows:
[0014] 1. The strong fat-soluble components of the two Codonopsis medicinal materials were developed with cyclohexane-ethyl acetate-formic acid in a volume ratio of 10:1:0.1. After development, they were examined under ultraviolet light at 365nm and 254nm. There were no information spots in both. After being sprayed with 10% sulfuric acid ethanol solution for color development, they were examined under ultraviolet light at 365nm. Codonopsis pilosula showed 9 fluorescent spots of different colors ( Figure 1 ); Codonopsis pilosula showed 8 fluorescent spots of different colors ( Figure 2 ), the difference between the two origins of Codonopsis pilosula is that Sichuan Codonopsis pilosula has one more fluorescent spot than Lu Codonopsis pilosula at an Rf value of about 0.69, and the remaining 8 fluorescent spots are completely consistent; when the thin layer plate is placed under sunlight for inspection, Sichuan Codonopsis pilosula shows 8 color spots; Lu Codonopsis pilosula shows 7 color spots. The difference between the two origins of Codonopsis pilosula is that Sichuan Codonopsis pilosula has one more color spot than Lu Codonopsis pilosula at an Rf value of about 0.69, and the remaining 7 color spots are completely consistent;
[0015] 2. The fat-soluble components of the two kinds of Codonopsis pilosula were developed with cyclohexane-ethyl acetate-formic acid in a volume ratio of 8:1.5:0.1. After development, they were examined under ultraviolet light at 365nm and 254nm. There were no information spots in both. After being sprayed with 10% sulfuric acid ethanol solution for color development, they were examined under ultraviolet light at 365nm. Both Sichuan Codonopsis pilosula and Lu Codonopsis pilosula showed 8 completely identical fluorescent spots ( Figure 3 ), the only difference is that the fluorescence intensity of some spots of Lu Codonopsis pilosula is slightly weaker than that of Chuan Codonopsis pilosula; when the thin layer plate is placed under sunlight for inspection, Chuan Codonopsis pilosula and Lu Codonopsis pilosula both show 7 spots of exactly the same color ( Figure 4 ), the only difference is that some spots of Lu Codonopsis are slightly lighter than those of Sichuan Codonopsis;
[0016] 3. The fat-soluble components of the two kinds of Codonopsis pilosula were developed with cyclohexane-ethyl acetate-formic acid in a volume ratio of 7:3:0.1. After development, they were examined under ultraviolet light at 365nm and 254nm. There were no information spots in both. After being sprayed with 10% sulfuric acid ethanol solution for color development, they were examined under ultraviolet light at 365nm. Both Sichuan Codonopsis pilosula and Lu Codonopsis pilosula showed 5 clear and identical main fluorescent spots ( Figure 5 ); When the thin layer plate was placed under sunlight for inspection, both Codonopsis pilosula and Codonopsis pilosula showed 7 spots of exactly the same color ( Figure 6 ); Under the two inspection conditions, the spots of the two Codonopsis components were basically the same;
[0017] 4. The polar components of the two kinds of Codonopsis pilosula were developed with cyclohexane-ethyl acetate-formic acid in a volume ratio of 3:7:0.1. After development, they were examined under ultraviolet light at 365nm and 254nm. There were no information spots in both. After being sprayed with 10% sulfuric acid ethanol solution for color development, they were examined under ultraviolet light at 365nm. Sichuan Codonopsis pilosula showed 6 fluorescent spots of different colors; Lu Codonopsis pilosula showed 7 fluorescent spots of different colors. The difference between the two kinds of Codonopsis pilosula is that Lu Codonopsis pilosula has one more blue-purple fluorescent spot at an Rf value of about 0.06 than Sichuan Codonopsis pilosula, and the other 6 fluorescent spots are basically the same ( Figure 7 ); When the thin layer plate was placed under sunlight for inspection, both Codonopsis pilosula and Codonopsis pilosula showed 7 spots of exactly the same color ( Figure 8 );
[0018] 5. The water-soluble components of the two kinds of Codonopsis pilosula were developed with chloroform-ethyl acetate-methanol-concentrated ammonia solution in a volume ratio of 2:5:3:0.5. After development, they were examined under ultraviolet light at 365nm and 254nm. There were no information spots in both. After being sprayed with 10% sulfuric acid ethanol solution for color development, they were examined under ultraviolet light at 365nm. Both Sichuan Codonopsis pilosula and Lu Codonopsis pilosula showed 4 clear and identical main fluorescent spots ( Fig. 9 ); When the thin layer plate was placed under sunlight for inspection, both Sichuan Codonopsis and Lu Codonopsis showed 5 spots of exactly the same color ( Fig.10 ); Under the two inspection conditions, the spots of the two Codonopsis components were basically the same;
[0019] 6. The water-soluble components of the two kinds of Codonopsis pilosula were developed with chloroform-ethyl acetate-methanol-concentrated ammonia solution in a volume ratio of 1.5:4:4:0.8. After development, they were examined under ultraviolet light at 365nm and 254nm. There were no information spots in both. After spraying with 10% sulfuric acid ethanol solution for color development, they were examined under ultraviolet light at 365nm. Both Sichuan Codonopsis pilosula and Lu Codonopsis pilosula showed three clear and identical main fluorescent spots ( Fig.11 ); When the thin layer plate was placed under sunlight for inspection, both Sichuan Codonopsis and Lu Codonopsis showed 5 spots of exactly the same color ( Fig.12 ); Under the two inspection conditions, the spots of the two Codonopsis components were basically the same;
[0020] 7. In 6 polarity gradient areas, under various inspection conditions, a total of 74 spots of component information were detected for Sichuan Codonopsis and 73 for Lu Codonopsis. Sichuan Codonopsis had 2 more spots of low-content components than Lu Codonopsis in the detection area (1). According to the Rf values of the fluorescence and color spots of 0.69, they should be the same components. In the detection area (4), there was 1 less fluorescent spot with poor stability. In fact, there were only 2 low-content components between the two Codonopsis. There were 71 similar spots with a similarity of 97.3% (71 / 73=97.3%). However, some of the same components of Sichuan Codonopsis were slightly higher than those of Lu Codonopsis. Although Sichuan Codonopsis and Lu Codonopsis have different origins and places of origin, they contain basically the same effective ingredients and have the same internal quality, which does not affect the quality of the raw materials. It provides a selection basis and a multi-region thin-layer identification exclusive method for compound preparations containing Codonopsis.
[0021] 8. The present invention found that: within the scope of the developing agent, no matter under sunlight, ultraviolet light 365nm, or 254nm, the components contained in the two kinds of Codonopsis pilosula have no information spots, and must be developed with sulfuric acid to stimulate the fluorescence of the components or present color spots. This discovery has broadened new information channels for the detection of Codonopsis pilosula, greatly increased the amount of detection information, and created an intrinsic quality evaluation method for the full information of Sichuan Codonopsis pilosula and Lu Codonopsis pilosula in multiple regions.
[0022] 9. The method is simple and quick. The medicinal materials only need to be ultrasonicated with methanol, the supernatant is spotted, developed, and inspected under different inspection conditions. A total of 0.1g of each medicinal material, 4ml of pretreatment solvent, 72ml of developing agent, and 3 hours are required to fully evaluate the internal quality of Sichuan Codonopsis and Lu Codonopsis in 6 areas. BRIEF DESCRIPTION OF THE DRAWINGS
[0023] Figure 1 The thin layer chromatograms of the strongly fat-soluble components of two Codonopsis pilosula medicinal materials were developed with 10% sulfuric acid ethanol solution and examined under ultraviolet light at 365nm.
[0024] Figure 2 The thin layer chromatograms of the strongly fat-soluble components of two Codonopsis pilosula medicinal materials were developed with 10% sulfuric acid ethanol solution and examined under sunlight.
[0025] Figure 3 The thin layer chromatograms of the fat-soluble components of two Codonopsis pilosula medicinal materials were developed with 10% sulfuric acid ethanol solution and examined under ultraviolet light at 365nm.
[0026] Figure 4 The thin layer chromatograms of the fat-soluble components of two Codonopsis pilosula medicinal materials were developed with 10% sulfuric acid ethanol solution and examined under sunlight.
[0027] Figure 5The thin layer chromatograms of the fat-soluble components of the two Codonopsis pilosula medicinal materials were developed with 10% sulfuric acid ethanol solution and then examined under ultraviolet light at 365nm.
[0028] Figure 6 This is a thin layer chromatogram of the fat-soluble components of two Codonopsis pilosula medicinal materials after being developed with 10% sulfuric acid ethanol solution and observed under sunlight.
[0029] Figure 7 The intermediate components of two Codonopsis pilosula medicinal materials were developed with 10% sulfuric acid ethanol solution and then examined under ultraviolet light at 365nm.
[0030] Figure 8 This is a thin layer chromatogram of the intermediate components of two Codonopsis pilosula medicinal materials after being developed with 10% sulfuric acid ethanol solution and observed under sunlight.
[0031] Fig. 9 The thin layer chromatograms of the water-soluble components of the two Codonopsis pilosula medicinal materials were developed with 10% sulfuric acid ethanol solution and examined under ultraviolet light at 365nm.
[0032] Fig.10 This is a thin layer chromatogram of the water-soluble components of two Codonopsis pilosula medicinal materials after being developed with 10% sulfuric acid ethanol solution and observed under sunlight.
[0033] Fig.11 The water-soluble components of two Codonopsis pilosula medicinal materials were developed with 10% sulfuric acid ethanol solution and then examined under ultraviolet light at 365nm.
[0034] Fig.12 This is a thin layer chromatogram of the water-soluble components of two Codonopsis pilosula medicinal materials after being developed with 10% sulfuric acid ethanol solution and examined under sunlight.
[0035] Figure 1 , 2 The chromatograms of the same thin layer plate under different inspection conditions are shown in the figure. 1.2.3 Radix Codonopsis pilosulae; 4.5.6 Radix Codonopsis pilosulae;
[0036] Figure 3 , 4 The chromatograms of the same thin layer plate under different inspection conditions are shown in the figure. 1.2.3 Radix Codonopsis pilosulae; 4.5.6 Radix Codonopsis pilosulae;
[0037] Figure 5 , 6 The chromatograms of the same thin layer plate under different inspection conditions are shown in the figure. 1.2.3 Radix Codonopsis pilosulae; 4.5.6 Radix Codonopsis pilosulae;
[0038] Figure 7 , 8 The chromatograms of the same thin layer plate under different inspection conditions are shown in the figure. 1.2.3 Radix Codonopsis pilosulae; 4.5.6 Radix Codonopsis pilosulae;
[0039] Fig. 9 , 10 The chromatograms of the same thin layer plate under different inspection conditions are shown in the figure. 1.2.3 Radix Codonopsis pilosulae; 4.5.6 Radix Codonopsis pilosulae;
[0040] Fig.11 , 12 These are chromatograms of the same thin layer plate under different inspection conditions. In the figure, 1.2.3 is Codonopsis pilosula; 4.5.6 is Codonopsis pilosula. DETAILED DESCRIPTION
[0041] (1) Comparison of the strong fat-soluble components of Sichuan Codonopsis and Lu Codonopsis medicinal materials. Take 0.1 g of Sichuan Codonopsis and Lu Codonopsis medicinal materials, add 2 ml of methanol, and treat them ultrasonically for 20 minutes. The supernatant is used as the test solution for each. Take 5-6 μl of each of the above two solutions and spot them on the same silica gel GF 254 On the thin layer plate, cyclohexane-ethyl acetate-formic acid with a volume ratio of 10:1:0.1 was used as the developing agent, developed, taken out, dried with hot air, sprayed with 10% sulfuric acid ethanol solution, heated at 105°C until the spots were clearly colored, and examined under a 365nm ultraviolet lamp. Sichuan Codonopsis showed 9 fluorescent spots of different colors; Lu Codonopsis showed 8 fluorescent spots of different colors. The only difference between the two Codonopsis was that Sichuan Codonopsis had one more fluorescent spot than Lu Codonopsis, and the remaining 8 fluorescent spots were completely consistent. The thin layer plate was examined under sunlight again. Sichuan Codonopsis showed 8 color spots; Lu Codonopsis showed 7 color spots. The difference between the two origins of Codonopsis was that Sichuan Codonopsis had one more color spot than Lu Codonopsis, and the remaining 7 color spots were completely consistent.
[0042] (2) Thin layer contrast analysis of fat-soluble components of Sichuan Codonopsis pilosula and Lu Codonopsis pilosula. 5 μl of the test solution under (1) was applied to the same silica gel GF 254 On the thin layer plate, cyclohexane-ethyl acetate-formic acid with a volume ratio of 8:1.5:0.1 was used as the developing agent, developed, taken out, dried with hot air, sprayed with 10% sulfuric acid ethanol solution, heated at 105℃ until the spots were clearly colored, and examined under a 365nm ultraviolet lamp. Both Sichuan Codonopsis and Lu Codonopsis showed 8 completely identical fluorescent spots, the only difference was that the fluorescence intensity of some spots of Lu Codonopsis was slightly weaker than that of Sichuan Codonopsis; the thin layer plate was examined under sunlight again, and both Sichuan Codonopsis and Lu Codonopsis showed 7 completely identical color spots, the only difference was that some color spots of Lu Codonopsis were slightly lighter than those of Sichuan Codonopsis;
[0043] (3) Thin layer contrast analysis of fat-soluble components of Sichuan Codonopsis pilosula and Lu Codonopsis pilosula. 5-6 μl of the test sample solution under (1) were applied to the same silica gel GF 254On the thin layer plate, cyclohexane-ethyl acetate-formic acid with a volume ratio of 7:3:0.1 was used as the developing agent, developed, taken out, dried with hot air, sprayed with 10% sulfuric acid ethanol solution, heated at 105℃ until the spots were clearly colored, and examined under a 365nm ultraviolet lamp. Both Sichuan Codonopsis and Lu Codonopsis showed 5 clear and identical main fluorescent spots; the thin layer plate was examined under sunlight again, and both Sichuan Codonopsis and Lu Codonopsis showed 7 completely identical color spots; under the two inspection conditions, the spots of the two Codonopsis components were basically the same;
[0044] (4) Thin layer contrast analysis of the polar components of Sichuan Codonopsis pilosula and Lu Codonopsis pilosula. 5-6 μl of the test solution under (1) were applied to the same silica gel GF 254 On the thin layer plate, cyclohexane-ethyl acetate-formic acid with a volume ratio of 3:7:0.1 was used as the developing agent, developed, taken out, dried with hot air, sprayed with 10% sulfuric acid ethanol solution, heated at 105°C until the spots were clearly colored, and examined under a 365nm ultraviolet lamp. Codonopsis pilosula showed 6 fluorescent spots of different colors; Codonopsis pilosula in Lu showed 7 fluorescent spots of different colors. The difference between the two Codonopsis pilosula was that Codonopsis pilosula in Lu had one more blue-purple fluorescent spot than Codonopsis pilosula in Sichuan, and the other 6 fluorescent spots were basically the same for the two Codonopsis pilosula. The thin layer plate was examined under sunlight again, and both Codonopsis pilosula in Sichuan and Codonopsis pilosula in Lu showed 7 completely identical color spots.
[0045] (5) Thin layer contrast analysis of water-soluble components of Sichuan Codonopsis pilosula and Lu Codonopsis pilosula. 5-6 μl of the test solution under (1) were applied to the same silica gel GF 254 On the thin layer plate, chloroform-ethyl acetate-methanol-concentrated ammonia solution with a volume ratio of 2:5:3:0.5 was used as the developing agent, developed, taken out, blown dry with hot air, sprayed with 10% sulfuric acid ethanol solution, heated at 105℃ until the spots were clearly colored, and examined under ultraviolet light at 365nm. Both Sichuan Codonopsis and Lu Codonopsis showed 4 clear and identical main fluorescent spots; the thin layer plate was examined under sunlight again, and both Sichuan Codonopsis and Lu Codonopsis showed 5 completely identical color spots; under the two inspection conditions, the spots of the two Codonopsis components were basically the same;
[0046] (6) Thin layer contrast analysis of water-soluble components of Sichuan Codonopsis pilosula and Lu Codonopsis pilosula. 5-6 μl of the test solution under (1) were applied to the same silica gel GF 254 On the thin layer plate, chloroform-ethyl acetate-methanol-concentrated ammonia test solution with a volume ratio of 1.5:4:4:0.8 was used as the developing agent. The plate was taken out, dried with hot air, sprayed with 10% sulfuric acid ethanol solution, heated at 105°C until the spots were clearly colored, and examined under ultraviolet light at 365nm. Both Sichuan Codonopsis and Lu Codonopsis showed 3 clear identical fluorescent main spots. The thin layer plate was examined under sunlight again. Both Sichuan Codonopsis and Lu Codonopsis showed 5 completely identical color spots. Under the two inspection conditions, the spots of the two Codonopsis components were basically the same.
Claims
1. A method for evaluating the quality of Sichuan Codonopsis pilosula and Lu Codonopsis pilosula with full information thin-layer chromatography from different sources and origins, characterized in that: (1) Comparison of the strong fat-soluble components of Sichuan Codonopsis and Lu Codonopsis medicinal materials. Take 0.1 g of Sichuan Codonopsis and Lu Codonopsis medicinal materials, add 2 ml of methanol, and treat them ultrasonically for 20 minutes. The supernatant is used as the test solution for each. Take 5-6 μl of each of the two test solutions and spot them on the same silica gel GF 254 On the thin layer plate, cyclohexane-ethyl acetate-formic acid with a volume ratio of 10:1:0.1 was used as the developing agent, developed, taken out, dried with hot air, sprayed with 10% sulfuric acid ethanol solution, heated at 105°C until the spots were clearly colored, and examined under a 365nm ultraviolet lamp. Sichuan Codonopsis showed 9 fluorescent spots of different colors; Lu Codonopsis showed 8 fluorescent spots of different colors. The only difference between the two Codonopsis was that Sichuan Codonopsis had one more fluorescent spot than Lu Codonopsis, and the remaining 8 fluorescent spots were completely consistent. The thin layer plate was examined under sunlight again. Sichuan Codonopsis showed 8 color spots; Lu Codonopsis showed 7 color spots. The difference between the two origins of Codonopsis was that Sichuan Codonopsis had one more color spot than Lu Codonopsis, and the remaining 7 color spots were completely consistent. (2) Thin layer contrast analysis of fat-soluble components of Sichuan Codonopsis pilosula and Lu Codonopsis pilosula. 5 μl of the test solution under (1) was applied to the same silica gel GF 254 On the thin layer plate, cyclohexane-ethyl acetate-formic acid with a volume ratio of 8:1.5:0.1 was used as the developing agent, developed, taken out, dried with hot air, sprayed with 10% sulfuric acid ethanol solution, heated at 105℃ until the spots were clearly colored, and examined under a 365nm ultraviolet lamp. Both Sichuan Codonopsis and Lu Codonopsis showed 8 completely identical fluorescent spots, the only difference was that the fluorescence intensity of some spots of Lu Codonopsis was slightly weaker than that of Sichuan Codonopsis; the thin layer plate was examined under sunlight again, and both Sichuan Codonopsis and Lu Codonopsis showed 7 completely identical color spots, the only difference was that some color spots of Lu Codonopsis were slightly lighter than those of Sichuan Codonopsis; (3) Thin layer contrast analysis of fat-soluble components of Sichuan Codonopsis pilosula and Lu Codonopsis pilosula. 5-6 μl of the test solution under (1) were applied to the same silica gel GF 254 On the thin layer plate, cyclohexane-ethyl acetate-formic acid with a volume ratio of 7:3:0.1 was used as the developing agent, developed, taken out, dried with hot air, sprayed with 10% sulfuric acid ethanol solution, heated at 105℃ until the spots were clearly colored, and examined under a 365nm ultraviolet lamp. Both Sichuan Codonopsis and Lu Codonopsis showed 5 clear and identical main fluorescent spots; the thin layer plate was examined under sunlight again, and both Sichuan Codonopsis and Lu Codonopsis showed 7 completely identical color spots; under the two inspection conditions, the spots of the two Codonopsis components were basically the same; (4) Thin layer contrast analysis of the polar components of Sichuan Codonopsis pilosula and Lu Codonopsis pilosula. 5-6 μl of the test solution under (1) were applied to the same silica gel GF 254 On the thin layer plate, cyclohexane-ethyl acetate-formic acid with a volume ratio of 3:7:0.1 was used as the developing agent, developed, taken out, dried with hot air, sprayed with 10% sulfuric acid ethanol solution, heated at 105°C until the spots were clearly colored, and examined under a 365nm ultraviolet lamp. Codonopsis pilosula showed 6 fluorescent spots of different colors; Codonopsis pilosula in Lu showed 7 fluorescent spots of different colors. The difference between the two Codonopsis pilosula was that Codonopsis pilosula in Lu had one more blue-purple fluorescent spot than Codonopsis pilosula in Sichuan, and the other 6 fluorescent spots were basically the same for the two Codonopsis pilosula. The thin layer plate was examined under sunlight again, and both Codonopsis pilosula in Sichuan and Codonopsis pilosula in Lu showed 7 completely identical color spots. (5) Thin layer contrast analysis of water-soluble components of Sichuan Codonopsis pilosula and Lu Codonopsis pilosula. 5-6 μl of the test solution under (1) were applied to the same silica gel GF 254 On the thin layer plate, chloroform-ethyl acetate-methanol-concentrated ammonia solution with a volume ratio of 2:5:3:0.5 was used as the developing agent, developed, taken out, blown dry with hot air, sprayed with 10% sulfuric acid ethanol solution, heated at 105℃ until the spots were clearly colored, and examined under ultraviolet light at 365nm. Both Sichuan Codonopsis and Lu Codonopsis showed 4 clear and identical main fluorescent spots; the thin layer plate was examined under sunlight again, and both Sichuan Codonopsis and Lu Codonopsis showed 5 completely identical color spots; under the two inspection conditions, the spots of the two Codonopsis components were basically the same; (6) Thin layer contrast analysis of water-soluble components of Sichuan Codonopsis pilosula and Lu Codonopsis pilosula. 5-6 μl of the test solution under (1) were applied to the same silica gel GF 254 On the thin layer plate, chloroform-ethyl acetate-methanol-concentrated ammonia solution with a volume ratio of 1.5:4:4:0.8 was used as the developing agent, and the plate was taken out, dried with hot air, sprayed with 10% sulfuric acid ethanol solution, heated at 105°C until the spots were clearly colored, and examined under a 365nm ultraviolet lamp. Both Sichuan Codonopsis and Lu Codonopsis showed 3 clear and identical main fluorescent spots; the thin layer plate was examined under sunlight again, and both Sichuan Codonopsis and Lu Codonopsis showed 5 completely identical color spots; under the two inspection conditions, the spots of the two Codonopsis components were basically the same; In the above 6 areas, ingredient information spots were detected under different inspection conditions: 74 spots from Sichuan Codonopsis pilosula and 73 spots from Lu Codonopsis pilosula. Sichuan Codonopsis pilosula had 2 more spots of low-content ingredients in the detection area (1) and 1 less fluorescent spot with poor stability in the detection area (4) than Lu Codonopsis pilosula. The similarity of the ingredients of the two Codonopsis pilosula was greater than 98.6%.
2. The method for evaluating the quality of Sichuan Codonopsis pilosula and Lu Codonopsis pilosula with full information of different origins and places of origin according to claim 1, characterized in that: The medicinal materials need to be ultrasonicated with methanol, the supernatant is spotted, developed, and inspected under different inspection conditions; a total of 0.1g of each medicinal material, 4ml of pretreatment solvent, 72ml of developing agent, and 3 hours are required to fully evaluate the intrinsic quality of Sichuan Codonopsis and Lu Codonopsis in 6 areas.
Citation Information
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