A traditional Chinese medicine composition for treating viral pneumonia of calves, a preparation method and application thereof

Abstract

This invention provides a traditional Chinese medicine composition for treating viral pneumonia in calves, its preparation method, and its application, belonging to the field of veterinary drug technology. The traditional Chinese medicine composition provided by this invention comprises the following raw materials in parts by weight: 15-20 parts of Gentiana scabra, 15-20 parts of Gardenia jasminoides, 15-20 parts of Scutellaria baicalensis, 10-15 parts of Bupleurum chinense, 25-35 parts of Rehmannia glutinosa, 25-35 parts of Plantago asiatica, 15-20 parts of Alisma plantago-aquatica, 25-35 parts of Akebia trifoliata, 10-15 parts of Angelica sinensis, 25-35 parts of Smilax glabra, 15-20 parts of Dioscorea hypoglauca, 25-35 parts of Dictamnus dasycarpus, and 10-15 parts of Glycyrrhiza uralensis (processed). The traditional Chinese medicine composition of this invention can effectively treat viral pneumonia in calves, and also has advantages that antibiotics cannot possess, such as being green and safe, residue-free, having few toxic side effects, low cost, convenient production, and being less likely to produce drug-resistant bacteria.

Description

Technical Field

[0001] This invention belongs to the field of veterinary drug technology, and in particular relates to a traditional Chinese medicine composition for treating viral pneumonia in calves, its preparation method, and its application. Background Technology

[0002] Viral pneumonia in calves is a group of diseases caused by viral infection. Calves become infected with viral pneumonia after birth when exposed to viruses (such as parainfluenza virus, bovine infectious rhinotracheitis virus, bovine adenovirus, bovine respiratory syncytial virus, etc.). This leads to pneumonia, and once inflammation occurs in the lungs, calves experience difficulty breathing, lethargy, loss of appetite or even complete anorexia, gradual weight loss, and decreased immunity. Sometimes, secondary bacterial infections can occur, causing digestive tract diseases such as diarrhea, further aggravating the condition. If left untreated, it can significantly impact the calf's health and growth, and in severe cases, lead to death. Currently, antibiotics are commonly used to treat viral pneumonia in calves. However, long-term, high-dose use of antibiotics can lead to problems such as drug resistance and drug residues. Summary of the Invention

[0003] In view of this, the purpose of this invention is to provide a traditional Chinese medicine composition for treating calf viral pneumonia, its preparation method and application, which has a good therapeutic effect on calf viral pneumonia and does not have problems such as drug resistance or drug residues.

[0004] To achieve the above-mentioned objectives, the present invention provides the following technical solution:

[0005] This invention provides a traditional Chinese medicine composition for treating viral pneumonia in calves, comprising the following raw materials in parts by weight: 15-20 parts of Gentiana scabra, 15-20 parts of Gardenia jasminoides, 15-20 parts of Scutellaria baicalensis, 10-15 parts of Bupleurum chinense, 25-35 parts of Rehmannia glutinosa, 25-35 parts of Plantago asiatica, 15-20 parts of Alisma plantago-aquatica, 25-35 parts of Akebia trifoliata, 10-15 parts of Angelica sinensis, 25-35 parts of Smilax glabra, 15-20 parts of Dioscorea hypoglauca, 25-35 parts of Dictamnus dasycarpus, and 10-15 parts of Glycyrrhiza uralensis (processed).

[0006] Preferred ingredients include the following parts by weight: 16-19 parts gentian root, 16-19 parts gardenia fruit, 16-19 parts scutellaria root, 11-14 parts bupleurum root, 28-32 parts rehmannia root, 28-32 parts plantain seed, 16-19 parts alisma rhizome, 28-32 parts akebia stem, 11-14 parts angelica root, 28-32 parts smilax rhizome, 16-19 parts dioscorea rhizome, 28-32 parts dictamnus root bark, and 11-14 parts prepared licorice root.

[0007] Preferred ingredients include the following parts by weight: 18 parts gentian root, 18 parts gardenia fruit, 18 parts scutellaria root, 12 parts bupleurum root, 30 parts rehmannia root, 30 parts plantain seed, 18 parts alisma rhizome, 30 parts akebia stem, 12 parts angelica root, 30 parts smilax rhizome, 18 parts dioscorea rhizome, 30 parts dictamnus root bark, and 12 parts prepared licorice root.

[0008] The present invention also provides a method for preparing the traditional Chinese medicine composition, comprising the following steps: mixing and decocting the traditional Chinese medicine with water twice, and combining the filtrates to obtain the traditional Chinese medicine composition.

[0009] Preferably, the amount of water used is 2-5 cm above the Chinese medicine, the decoction temperature is 80-120℃, and the decoction time is 10-25 min / time.

[0010] The present invention also provides the application of the traditional Chinese medicine composition described herein or the traditional Chinese medicine composition prepared by the described preparation method in the preparation of a drug for treating viral pneumonia in calves.

[0011] Compared with the prior art, the present invention has the following beneficial effects:

[0012] The traditional Chinese medicine composition of this invention can effectively treat viral pneumonia in calves, with a cure rate of 93.33% and a total effective rate of 96.67%. It also possesses advantages that antibiotics cannot match, such as being green and safe, residue-free, having few toxic side effects, low cost, convenient production, and being less prone to producing drug-resistant bacteria. Detailed Implementation

[0013] This invention provides a traditional Chinese medicine composition for treating viral pneumonia in calves, comprising the following raw materials in parts by weight: 15-20 parts of Gentiana scabra, 15-20 parts of Gardenia jasminoides, 15-20 parts of Scutellaria baicalensis, 10-15 parts of Bupleurum chinense, 25-35 parts of Rehmannia glutinosa, 25-35 parts of Plantago asiatica, 15-20 parts of Alisma plantago-aquatica, 25-35 parts of Akebia trifoliata, 10-15 parts of Angelica sinensis, 25-35 parts of Smilax glabra, 15-20 parts of Dioscorea hypoglauca, 25-35 parts of Dictamnus dasycarpus, and 10-15 parts of Glycyrrhiza uralensis (processed).

[0014] In this invention, the preferred dosage of gentian is 16-19 parts, more preferably 18 parts; gentian is bitter and cold; it enters the liver and gallbladder meridians; it has the effects of clearing heat and drying dampness, and purging liver and gallbladder fire.

[0015] The preferred dosage of gardenia is 16-19 parts, more preferably 18 parts; gardenia, also known as yellow gardenia, mountain gardenia, and white toad, is bitter and cold in nature; it enters the heart, lung, and triple burner meridians. It has the effects of purging fire and relieving irritability, clearing heat and promoting diuresis, cooling blood and detoxifying, and externally reducing swelling and relieving pain.

[0016] The preferred dosage of Scutellaria baicalensis is 16-19 parts, more preferably 18 parts. Scutellaria baicalensis is bitter and cold in nature. It enters the lung, gallbladder, spleen, large intestine, and small intestine meridians. Scutellaria baicalensis is bitter, cold, and drying, primarily entering the lung and large intestine meridians, and also the gallbladder, spleen, and stomach meridians. It clears heat and purges fire while cooling the blood and stopping bleeding, calming the fetus, and relieving heat toxicity. It also dries dampness and removes moisture to relieve damp-heat toxicity. It is an essential medicine for treating damp-heat and fire toxicity and is widely used for symptoms of damp-heat and fire toxicity.

[0017] The preferred dosage of Bupleurum is 11-14 parts, more preferably 12 parts; Bupleurum is pungent and bitter in taste, and slightly cold in nature. It has the effects of harmonizing the exterior and interior, soothing the liver and relieving depression, raising yang and lifting prolapse, and reducing fever and stopping malaria.

[0018] The preferred dosage of Rehmannia glutinosa is 28-32 parts, more preferably 30 parts; Rehmannia glutinosa is sweet in taste and cold in nature, and enters the heart, liver, and kidney meridians. It has the effects of clearing heat and cooling blood, nourishing yin, and promoting body fluid production.

[0019] The preferred dosage of Plantago asiatica seeds is 28-32 parts, more preferably 30 parts. Plantago asiatica seeds are sweet in taste and cold in nature, and enter the liver, kidney, lung, and small intestine meridians. They have the effects of clearing heat and promoting diuresis, relieving strangury, eliminating dampness and stopping diarrhea, improving eyesight, and eliminating phlegm.

[0020] The preferred dosage of Alisma plantago-aquatica is 16-19 parts, more preferably 18 parts; Alisma plantago-aquatica is sweet and bland in taste, cold in nature, and enters the kidney and bladder meridians. It has the effects of promoting diuresis and eliminating dampness, clearing heat, resolving turbidity and lowering lipids.

[0021] The preferred dosage of Aristolochia debilis is 28-32 parts, more preferably 30 parts. Aristolochia debilis is bitter and cold in nature, and enters the heart, small intestine, and bladder meridians. It has the effects of promoting diuresis and relieving strangury, clearing the heart and relieving irritability, and promoting menstruation and lactation.

[0022] The preferred dosage of Angelica sinensis is 11-14 parts, more preferably 12 parts; Angelica sinensis is sweet, pungent, and warm in nature, and enters the liver, heart, and spleen meridians. It has the effects of nourishing blood and promoting blood circulation, regulating menstruation and relieving pain, and moistening the intestines and promoting bowel movements.

[0023] The preferred dosage of Smilax glabra is 28-32 parts, more preferably 30 parts; Smilax glabra is sweet, bland, and neutral in nature, and enters the liver and stomach meridians. It has the effects of detoxification, dampness removal, and joint mobility promotion.

[0024] The preferred dosage of Dioscorea hypoglauca is 16-19 parts, and more preferably 18 parts. Dioscorea hypoglauca is bitter and neutral in nature, and enters the kidney and stomach meridians. It has the effects of promoting diuresis and removing turbidity, dispelling wind and relieving numbness.

[0025] The preferred dosage of Dictamnus dasycarpus root bark is 28-32 parts, more preferably 30 parts; Dictamnus dasycarpus root bark is bitter and cold in nature. It enters the spleen, stomach, and bladder meridians. It has the effects of clearing heat and drying dampness, dispelling wind and detoxifying.

[0026] The preferred dosage of the prepared licorice root is 11-14 parts, more preferably 12 parts. Prepared licorice root is neutral in nature and has the effects of invigorating qi and restoring pulse, tonifying the spleen and stomach, and harmonizing the properties of other herbs.

[0027] The present invention also provides a method for preparing the traditional Chinese medicine composition, comprising the following steps: mixing and decocting the traditional Chinese medicine with water twice, and combining the filtrates to obtain the traditional Chinese medicine composition.

[0028] In this invention, the amount of water used is preferably 2-5 cm above the Chinese herbal medicine, more preferably 3-4 cm above the Chinese herbal medicine; the decoction temperature is preferably 80-120℃, more preferably 90-110℃; and the decoction time is preferably 10-25 min / time, more preferably 12-22 min / time.

[0029] The present invention also provides the application of the traditional Chinese medicine composition described herein or the traditional Chinese medicine composition prepared by the described preparation method in the preparation of a drug for treating viral pneumonia in calves.

[0030] The technical solutions provided by the present invention will be described in detail below with reference to the embodiments, but they should not be construed as limiting the scope of protection of the present invention.

[0031] Example 1

[0032] A traditional Chinese medicine composition for treating viral pneumonia in calves, comprising the following raw materials in parts by weight: 18 parts gentian root, 18 parts gardenia fruit, 18 parts scutellaria root, 12 parts bupleurum root, 30 parts rehmannia root, 30 parts plantain seed, 18 parts alisma rhizome, 30 parts akebia stem, 12 parts angelica root, 30 parts smilax rhizome, 18 parts dioscorea rhizome, 30 parts dictamnus root bark, and 12 parts prepared licorice root.

[0033] The preparation method of the traditional Chinese medicine composition is as follows: mix the traditional Chinese medicine and water that covers the traditional Chinese medicine by 3 cm, decoct at 100°C for 15 min, decoct the filtered residue again according to the above operation, and combine the filtrates after the two decoctions to obtain the traditional Chinese medicine composition.

[0034] Example 2

[0035] A traditional Chinese medicine composition for treating viral pneumonia in calves, comprising the following raw materials in parts by weight: 15 parts gentian root, 15 parts gardenia fruit, 15 parts scutellaria root, 10 parts bupleurum root, 25 parts rehmannia root, 25 parts plantain seed, 15 parts alisma rhizome, 25 parts akebia stem, 10 parts angelica root, 25 parts smilax rhizome, 15 parts dioscorea rhizome, 25 parts dictamnus root bark, and 10 parts prepared licorice root.

[0036] The preparation method of the traditional Chinese medicine composition is as follows: mix the traditional Chinese medicine with water that covers the traditional Chinese medicine by 2 cm and decoct at 80°C for 10 minutes. Then, decoct the filtered residue again according to the above operation. Combine the filtrates from the two decoctions to obtain the traditional Chinese medicine composition.

[0037] Example 3

[0038] A traditional Chinese medicine composition for treating viral pneumonia in calves, comprising the following raw materials in parts by weight: 20 parts gentian root, 20 parts gardenia fruit, 20 parts scutellaria root, 15 parts bupleurum root, 35 parts rehmannia root, 35 parts plantain seed, 20 parts alisma rhizome, 35 parts akebia stem, 15 parts angelica root, 35 parts smilax rhizome, 20 parts dioscorea rhizome, 35 parts dictamnus root bark, and 15 parts prepared licorice root.

[0039] The preparation method of the traditional Chinese medicine composition is as follows: the traditional Chinese medicine and water covering the traditional Chinese medicine by 5 cm are mixed and decocted at 120°C for 25 minutes. The dregs obtained by filtration are decocted again according to the above operation. The filtrates after the two decoctions are combined to obtain the traditional Chinese medicine composition.

[0040] Experimental Example 1

[0041] 1. Epidemiological survey of calves at a certain farm:

[0042] Sick calves: 3 months old

[0043] The calf coughed, had abnormal sounds in its lungs, and was accompanied by symptoms such as fever, diarrhea, and loss of appetite.

[0044] The temperature varies greatly.

[0045] 2. Blood routine examination of the sick calf:

[0046] Table 1. Results of routine blood tests in diseased calves

[0047]

[0048] Note: The data in bold is higher than the reference range, the data in underline is lower than the reference range, and "-" indicates no data was detected.

[0049] 3. Blood biochemical indicators of diseased calves:

[0050] Table 2. Results of Blood Biochemical Indicators in Diseased Calves

[0051]

[0052] Note: The data in bold is higher than the reference range, and the data in underline is lower than the reference range.

[0053] 4. Feeding method:

[0054] For a calf weighing 120kg, take one dose of the traditional Chinese medicine composition prepared in Example 1 per day, and take two doses as one course of treatment, for a total of two courses of treatment.

[0055] 5. Prognostic effect: As shown in Tables 3 and 4, Table 3 shows that after taking the traditional Chinese medicine composition of the present invention, the lymphocytes, hemoglobin content and platelet mean volume of the blood routine test results that were high returned to the normal range. Although hemoglobin, hemoglobin concentration and total platelet count did not return to the normal range, they also decreased significantly. The total red blood cell count and red blood cell distribution width that were low also returned to the normal range.

[0056] As shown in Table 4, after taking the traditional Chinese medicine composition of the present invention, the values ​​of total protein and aspartate transferase, which were previously high in blood biochemical indicators, have returned to the normal range, and the values ​​of triglycerides, which were previously low, have also reached the normal range.

[0057] Table 3. Results of routine blood tests in sick calves after medication.

[0058]

[0059] Table 4. Results of blood biochemical indicators in sick calves after medication.

[0060]

[0061] Experiment Example 2

[0062] Clinical efficacy trial

[0063] Clinical observation data: A total of 60 calves, aged 60-90 days, were tested. They were randomly divided into two groups: an experimental group and a control group, with 30 calves in each group. There were no significant differences between the groups.

[0064] Experimental Methods: The experimental group received the traditional Chinese medicine composition prepared in Example 1, one dose per day, with two doses constituting one course of treatment, for a total of two courses. The control group received florfenicol. The therapeutic effect of the traditional Chinese medicine composition of this invention on viral pneumonia in calves was observed.

[0065] Diagnostic criteria: Clinical features include depression, decreased appetite, and elevated body temperature; lying prone on the ground, coughing, increased respiratory rate, abdominal breathing, dry or wet rales on auscultation of the lungs, and in severe cases, mouth breathing, cough of varying degrees, dyspnea, or even respiratory failure.

[0066] During the clinical trial, namely before administration, during administration, and for 3 days after discontinuation, the number of coughs, respiratory rate, whether panting was obvious, decrease or increase in appetite, whether body temperature was elevated, and other systemic symptoms such as mental state of the test calves were observed and recorded once a day, and whether any deaths occurred.

[0067] Judgment criteria:

[0068] Recovery: Symptoms disappeared after medication; mental state and appetite returned to normal; body temperature was normal; there was no cough; and respiratory rate was normal. Auscultation revealed normal vesicle sounds, with no dry or wet rales heard.

[0069] Significant effect: After medication, symptoms are significantly relieved, mental state improves, appetite returns to normal, body temperature decreases, cough frequency is significantly reduced, respiratory rate is basically normal, and occasional dry or wet rales can be heard on auscultation of the lungs.

[0070] Effective: Symptoms are relieved after medication, mental state and appetite improve, body temperature decreases, cough frequency decreases, respiratory rate decreases compared to when medication was not administered, and auscultation reveals a small number of dry or moist rales in the lungs.

[0071] Invalid criteria: No change in symptoms after medication, or worsening of symptoms, or death.

[0072] The dosage was calculated based on the weight of the calf and administered orally. The medication was given twice daily, once before morning and once before afternoon feeding, for 5 consecutive days. After discontinuing the medication, the calves were observed for another 3 days. Florfenicol was used as a positive control group. During the treatment period, the calves were allowed free access to food and water.

[0073] Treatment Results: In the treatment of naturally occurring viral pneumonia in calves, the number of calves that recovered, showed significant improvement, showed improvement, and showed no improvement were 28, 0, 1, and 1 respectively, with a cure rate of 93.33% and a total effective rate of 96.67%. In the control group, the number of calves that recovered, showed significant improvement, showed improvement, and showed no improvement were 28, 1, 0, and 1 respectively, with a cure rate of 93.33% and a total effective rate of 96.67%. It can be seen that the traditional Chinese medicine composition of this invention has a therapeutic effect on calf pneumonia, can alleviate symptoms, and reduce mortality. The traditional Chinese medicine composition of this invention can achieve the effects of treating the disease and improving survival rate, with no significant difference in clinical cure rate and total effective rate (P>0.05). No adverse reactions were found during the clinical trial, and the therapeutic effect was comparable to that of florfenicol.

[0074] As can be seen from the above embodiments and experimental examples, the traditional Chinese medicine composition of the present invention has the functions of clearing heat and detoxifying, clearing the lungs and relieving cough, and relieving phlegm and asthma, and can effectively treat viral pneumonia in calves.

[0075] The above description is only a preferred embodiment of the present invention. It should be noted that for those skilled in the art, several improvements and modifications can be made without departing from the principle of the present invention, and these improvements and modifications should also be considered within the scope of protection of the present invention.

Claims

1. A traditional Chinese medicine composition for treating viral pneumonia in calves, characterized in that, It is made from the following raw materials in parts by weight: 15-20 parts gentian root, 15-20 parts gardenia fruit, 15-20 parts scutellaria root, 10-15 parts bupleurum root, 25-35 parts rehmannia root, 25-35 parts plantain seed, 15-20 parts alisma rhizome, 25-35 parts akebia stem, 10-15 parts angelica root, 25-35 parts smilax rhizome, 15-20 parts dioscorea rhizome, 25-35 parts dictamnus root bark, and 10-15 parts prepared licorice root.

2. The traditional Chinese medicine composition according to claim 1, characterized in that, It is made from the following raw materials in parts by weight: 16-19 parts gentian root, 16-19 parts gardenia fruit, 16-19 parts scutellaria root, 11-14 parts bupleurum root, 28-32 parts rehmannia root, 28-32 parts plantain seed, 16-19 parts alisma rhizome, 28-32 parts akebia stem, 11-14 parts angelica root, 28-32 parts smilax rhizome, 16-19 parts dioscorea rhizome, 28-32 parts dictamnus root bark, and 11-14 parts prepared licorice root.

3. The traditional Chinese medicine composition according to claim 1, characterized in that, It is made from the following ingredients in parts by weight: 18 parts gentian root, 18 parts gardenia fruit, 18 parts scutellaria root, 12 parts bupleurum root, 30 parts rehmannia root, 30 parts plantain seed, 18 parts alisma rhizome, 30 parts akebia stem, 12 parts angelica root, 30 parts smilax rhizome, 18 parts dioscorea rhizome, 30 parts dictamnus root bark, and 12 parts prepared licorice root.

4. The method for preparing the traditional Chinese medicine composition according to claim 1, characterized in that, Includes the following steps: The Chinese herbal medicine was decocted twice with water, and the filtrates were combined to obtain the Chinese herbal medicine composition.

5. The preparation method according to claim 4, characterized in that, The amount of water used is 2-5 cm above the Chinese medicine, the decoction temperature is 80-120℃, and the decoction time is 10-25 min per batch.

6. The use of the traditional Chinese medicine composition according to any one of claims 1 to 3 or the traditional Chinese medicine composition prepared by the preparation method according to claim 4 or 5 in the preparation of a drug for treating viral pneumonia in calves.

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