A set of large leaf white hemp hypocotyl regeneration medium and regeneration method

By scientifically formulating the regeneration culture medium for large-leaf white hemp and adding organic additives, the problems of habitat destruction and long seedling time in the propagation of large-leaf white hemp have been solved, realizing a rapid and effective regeneration method, promoting the proliferation of callus tissue and adventitious buds, and improving seedling efficiency.

CN118633520BActive Publication Date: 2026-06-19TARIM UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
TARIM UNIV
Filing Date
2024-06-12
Publication Date
2026-06-19

AI Technical Summary

Technical Problem

Existing propagation methods for large-leaf white hemp destroy the natural habitat and take a long time to develop seedlings. There is a lack of methods that can produce seedlings quickly without destroying the habitat.

Method used

The following regeneration media for large-leaved white hemp, using hypocotyls as explants, include callus induction medium, somatic embryo formation and seedling growth medium, solid-liquid bilayer adventitious bud proliferation medium, and seedling rooting medium. Through scientific formulation and the addition of organic additives, the induction rate of callus tissue and the proliferation coefficient of adventitious buds are improved, the browning rate is reduced, and somatic embryo formation and rooting are promoted.

Benefits of technology

This method significantly shortens seedling time, improves callus induction rate and adventitious bud proliferation efficiency without damaging the natural habitat, promotes somatic embryo formation and rooting, and provides a rapid regeneration method.

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Abstract

This invention relates to a set of regeneration media and methods for large-leaved white hemp using hypocotyls as explants, belonging to the field of plant tissue culture technology. This invention provides a set of regeneration media for large-leaved white hemp using hypocotyls as explants. These media include callus induction medium, somatic embryogenesis and seedling formation medium, a solid-liquid bilayer adventitious bud proliferation medium, and a seedling rooting medium. The scientifically and effectively proportioned media of this invention significantly improve the callus induction rate and adventitious bud proliferation coefficient, reduce the browning rate of callus and somatic embryos, and shorten the seedling emergence time by inducing callus to form somatic embryos. This provides a new approach for subsequent genetic transformation, tissue culture propagation, and virus-free germplasm research in large-leaved white hemp.
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