Plant tissue culture sterile operation box

Abstract

The utility model relates to aseptic operation technical field, specifically disclose plant tissue culture aseptic operation box, including box, the box is transparent, the both sides of the end of box all are provided with operation hole, the inside fixed connection sealing ring of two operation holes, the inboard fixed connection sterile gloves of sealing ring, the inside fixed connection support base of box, the top fixed mounting of support base has the culture plate, the middle fixed mounting of culture plate several groups of culture dish, the both sides of culture plate swing set the batten, two battens set up at the bottom of sterile gloves, the inside preset heat conduction structure of batten. Through setting rotatable batten at the top both sides of culture plate, for the sterile gloves in the box is realized to hold, avoid the free falling form of sterile gloves, the tail end of sterile gloves is in the flush posture with operation hole at this time, in order to facilitate the later wearing of operating personnel.

Description

Technical Field

[0001] This utility model relates to the field of aseptic operation technology, and in particular to an aseptic operation box for plant tissue culture. Background Technology

[0002] Plant tissue culture refers to the technique of culturing isolated plant tissues, organs, cells, or protoplasts on a culture medium containing various nutrients and plant hormones using aseptic techniques, allowing them to grow, differentiate, and develop into complete plants.

[0003] Currently, all existing aseptic operating boxes on the market have a pair of sterile gloves built in. These sterile gloves hang freely inside the operating box, which means that when operators put them on, they need to insert their hands into the box through the operating hole and lift the gloves to complete the putting-on process. In addition, if there are sharp parts inside the incubator (such as the edge of the culture plate), the gloves may be damaged. Utility Model Content

[0004] The purpose of this section is to outline some aspects of embodiments of the present invention and to briefly describe some preferred embodiments. Simplifications or omissions may be made in this section, as well as in the abstract and title of this application, to avoid obscuring the purpose of these documents; however, such simplifications or omissions should not be construed as limiting the scope of the present invention.

[0005] This utility model provides a sterile operating box for plant tissue culture, which can solve the problem of gloves easily being damaged inside the box. The specific solution is as follows:

[0006] A sterile operating box for plant tissue culture includes a transparent box body with operating holes on both sides of its ends. A sealing ring is fixedly connected inside each of the two operating holes, and a sterile glove is fixedly connected to the inner side of the sealing ring. A support base is fixedly connected inside the box body, with a culture plate fixedly mounted on the top of the support base. Several sets of culture dishes are fixedly mounted on the middle of the culture plate. Two mounting plates are movably arranged on both sides of the culture plate, with two mounting plates positioned at the bottom of the sterile glove. The mounting plates have a pre-designed heat-conducting structure inside.

[0007] As a preferred embodiment of this utility model, rotating rods are connected to both sides of the culture plate. The middle part of the rotating rod is fixed to the tail end of the plate through a sleeve. A gear structure is fixedly connected to the top of the rotating rod. The gear structure consists of two meshing bevel gears.

[0008] As a preferred embodiment of this utility model, a vertical rod is fixedly connected to the top side of the support base, and a motor is fixedly installed at the top of the vertical rod. The output end of the motor corresponds to the central axis of one of the bevel gears, and the output end of the motor is fixedly connected to the bevel gear.

[0009] As a preferred embodiment of this utility model, the heat-conducting structure includes a convex plate disposed on the side wall of the mounting plate. A cavity is formed inside the convex plate. A heat-conducting plate is fixedly connected to the top wall of the cavity. Multiple heating wires are fixedly installed inside the cavity. The surface of the heat-conducting plate is in contact with the bottom wall of the sterile glove.

[0010] As a preferred technical solution of this utility model, a controller is fixedly connected to the outside of the box, a docking plate is fixedly connected to the outside of the box, an ultraviolet lamp is fixedly installed at one end of the docking plate, the ultraviolet lamp is located inside the box and is located at the top of the culture plate, and the other end of the ultraviolet lamp is electrically connected to the controller through a wire.

[0011] As a preferred technical solution of this utility model, side plates are fixedly connected to the bottom of both sides of the box body, and movable wheels are fixedly connected to the bottom sides of both side plates, and locking structures are preset at the tails of all four movable wheels.

[0012] As a preferred embodiment of this utility model, an opening and closing door is provided on one side of the front of the box.

[0013] Compared with the prior art, the present invention can achieve at least one of the following beneficial effects:

[0014] By setting rotatable support plates on both sides of the top of the culture plate, the sterile gloves inside the box can be supported to prevent them from hanging freely. At this time, the cuff of the sterile glove is flush with the operation hole, so that the operator can wear it later.

[0015] Because the sterile gloves are lifted up, they avoid contact with the edges of the culture plate or plate, thus improving the protective properties of the sterile gloves and preventing damage to the gloves.

[0016] Other features and advantages of this invention will be set forth in the description which follows, and will be apparent in part from the description, or may be learned by practicing the invention. The objects and other advantages of this invention can be realized and obtained by means of the structures particularly pointed out in the written description and the accompanying drawings. Attached Figure Description

[0017] To more clearly illustrate the technical solutions of the embodiments of this utility model, the drawings used in the description of the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort. Among them:

[0018] Figure 1 This is a schematic diagram of the overall structure of this utility model;

[0019] Figure 2 This is a schematic diagram of the culture plate of this utility model;

[0020] Figure 3 This is a schematic diagram of the mounting plate structure of this utility model;

[0021] Figure 4 This is a schematic diagram of the internal structure of the mounting plate of this utility model;

[0022] The accompanying figure is labeled as follows:

[0023] 1. Box body; 2. Side panel; 3. Casters; 4. Operating hole; 5. Sealing ring; 6. Sterile gloves; 7. Controller; 8. Support base; 9. Docking plate; 10. Ultraviolet lamp; 11. Mounting plate; 12. Culture plate; 13. Petri dish; 14. Gear structure; 15. Motor; 16. Rotating rod; 17. Vertical rod; 18. Heat-conducting plate; 19. Heating wire; 20. Cavity. Detailed Implementation

[0024] The preferred embodiments of the present invention will now be described in detail with reference to the accompanying drawings, which constitute a part of the present invention and, together with the embodiments of the present invention, serve to illustrate the principles of the present invention.

[0025] See Figures 1-4 This utility model provides a sterile operating box for plant tissue culture, including a box body 1, which is transparent. The box body 1 has operating holes 4 on both sides of its ends. A sealing ring 5 is fixedly connected inside the two operating holes 4, and a sterile glove 6 is fixedly connected inside the sealing ring 5. A support base 8 is fixedly connected inside the box body 1. A culture plate 12 is fixedly installed at the top of the support base 8. Several sets of culture dishes 13 are fixedly installed in the middle of the culture plate 12. The two sides of the culture plate 12 are movably provided with mounting plates 11. The two mounting plates 11 are set at the bottom of the sterile glove 6. The mounting plates 11 have a pre-set heat-conducting structure inside.

[0026] Rotating rods 16 are connected to both sides of the culture plate 12. The middle part of the rotating rod 16 is fixed to the tail end of the mounting plate 11 through a sleeve. The top end of the rotating rod 16 is fixedly connected to a gear structure 14, which consists of two meshing bevel gears.

[0027] A vertical rod 17 is fixedly connected to the top side of the support base 8. A motor 15 is fixedly installed at the top of the vertical rod 17. The output end of the motor 15 corresponds to the central axis of one of the bevel gears, and the output end of the motor 15 is fixedly connected to the bevel gear. When in use, the motor 15 can be started, and the motor 15 drives the gear structure 14 to rotate. Therefore, the connecting plate 11 connected to it rotates accordingly, so that the connecting plate 11 is at the bottom of the sterile glove 6 and lifts the sterile glove 6.

[0028] After the sterile gloves 6 are put on, the motor 15 can be restarted to retract the plate 11, so that it is parallel to the culture plate 12, thereby saving space and not affecting the operation and processing on the culture plate 12.

[0029] The heat-conducting structure includes a convex plate, which is disposed on the side wall of the mounting plate 11. A cavity 20 is opened inside the convex plate. A heat-conducting plate 18 is fixedly connected to the top wall of the cavity 20. Multiple heating wires 19 are fixedly installed inside the cavity 20. The surface of the heat-conducting plate 18 is in contact with the bottom wall of the sterile glove 6 to provide a preheating effect for the sterile glove 6, making it easier for the user to wear it later. It should be noted that the maximum temperature of the heating wires 19 does not exceed 40°. This is designed to avoid direct damage to the surface of the sterile glove 6 by high temperature.

[0030] The controller 7 is fixedly connected to the outside of the box 1, and the docking plate 9 is fixedly connected to the outside of the box 1. One end of the docking plate 9 is fixedly installed with an ultraviolet lamp 10. The ultraviolet lamp 10 is located inside the box 1 and is located on the top of the culture plate 12. The other end of the ultraviolet lamp 10 is electrically connected to the controller 7 through a wire.

[0031] By installing ultraviolet lamps 10 inside the chamber 1, the ultraviolet light emitted by the lamps 10 can directly act on the genetic material of microorganisms, namely DNA (deoxyribonucleic acid) or RNA (ribonucleic acid). The ultraviolet lamps 10 operate continuously, killing microorganisms that may fall onto the surfaces of these items and preventing contamination. For example, in cell culture experiments, the cell culture medium, culture flasks, and other items in the sterile chamber are disinfected with ultraviolet light, reducing the chance of microbial contamination of cells and ensuring the smooth progress of the experiment.

[0032] Side plates 2 are fixedly connected to the bottom of both sides of the box 1. Movable wheels 3 are fixedly connected to the bottom of both sides of the two side plates 2. The tail of each of the four movable wheels 3 has a pre-set locking structure. When the entire box 1 needs to be moved, the locking structure at the tail of the movable wheel 3 is unlocked, allowing the movable wheel 3 to move freely. Therefore, the four movable wheels 3 at the bottom can drive the box 1 to complete the transportation movement.

[0033] The movement of the container 1 itself has been improved, enabling it to move quickly according to the location where it needs to be operated.

[0034] A door is provided on one side of the front of the box 1 for placing plant tissues or inspecting the internal structure later.

[0035] When in use, the user can insert both hands into the two operating holes 4 and forcefully pass them through the sealing ring 5 to put on a pair of sterile gloves 6 inside the box 1. At this time, the sterile gloves 6 are on top of the plate 11, so the cuffs of the sterile gloves 6 are flush with the operating holes 4, which makes it easy for operators to put on and reduces the occurrence of damage to the sterile gloves 6. Since the entire box 1 is a transparent structure, after wearing the sterile gloves 6, the internal structure of the box 1 can be directly observed and corresponding operations can be performed.

[0036] In the description of this specification, references to terms such as "an embodiment," "example," "specific example," etc., indicate that a specific feature, structure, material, or characteristic described in connection with that embodiment or example is included in at least one embodiment or example of the present invention. In this specification, the illustrative expressions of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples.

[0037] The terms "first," "second," "third," "fourth," etc. (if present) in the specification, claims, and accompanying drawings of this application are used to distinguish similar objects and are not necessarily used to describe a specific order or sequence. It should be understood that such data can be interchanged where appropriate so that embodiments of the present application described herein can be implemented, for example, in orders other than those illustrated or described herein. Furthermore, the terms "comprising" and "having," and any variations thereof, are intended to cover non-exclusive inclusion; for example, a process, method, system, product, or apparatus that comprises a series of steps or units is not necessarily limited to those explicitly listed, but may include other steps or units not explicitly listed or inherent to such processes, methods, products, or apparatus.

[0038] The devices or elements referred to in the embodiments of this application or implied herein must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be construed as limiting the embodiments of this application. In the description of the embodiments of this application, "a plurality of" means two or more, unless otherwise precisely specified.

[0039] The preferred embodiments of this utility model disclosed above are merely illustrative of the present utility model. These preferred embodiments do not exhaustively describe all details, nor do they limit the utility model to the specific implementations described. Clearly, many modifications and variations can be made based on the content of this specification. This specification selects and specifically describes these embodiments to better explain the principles and practical applications of this utility model, thereby enabling those skilled in the art to better understand and utilize it. This utility model is limited only by the claims and their full scope and equivalents.

Claims

1. A plant tissue culture sterile operating box comprising a box body (1), characterized in that: The box (1) is transparent, both sides of the end of the box (1) are provided with operation holes (4), the inside of the two operation holes (4) is fixedly connected with sealing rings (5), the inside of the sealing ring (5) is fixedly connected with sterile gloves (6); the inside of the box (1) is fixedly connected with a support seat (8), the top end of the support seat (8) is fixedly installed with a culture plate (12), the middle part of the culture plate (12) is fixedly installed with a plurality of groups of culture dishes (13), both sides of the culture plate (12) are movably provided with clamping plates (11), the two clamping plates (11) are arranged at the bottom of the sterile gloves (6), and the inside of the clamping plate (11) is provided with a heat conduction structure.

2. The plant tissue culture sterile operating box according to claim 1, wherein: Both sides of the culture plate (12) are connected with rotating rods (16), the middle part of the rotating rod (16) is fixedly connected with the tail end of the clamping plate (11) through a sleeve, the top end of the rotating rod (16) is fixedly connected with a gear structure (14), and the gear structure (14) is composed of two intermeshing bevel gears.

3. The plant tissue culture sterile operating box according to claim 2, wherein: The top side of the support seat (8) is fixedly connected with a vertical rod (17), the top end of the vertical rod (17) is fixedly installed with a motor (15), the output end of the motor (15) corresponds to the middle axis of one of the bevel gears, and the output end of the motor (15) is fixedly connected with the bevel gear.

4. The plant tissue culture sterile operating box according to claim 1, wherein: The heat conduction structure comprises a protruding plate arranged on the side wall of the clamping plate (11), a cavity (20) is formed in the inside of the protruding plate, a heat conduction plate (18) is fixedly connected to the top wall of the cavity (20), a plurality of heating wires (19) are fixedly installed in the inside of the cavity (20), and the surface of the heat conduction plate (18) is attached to the bottom wall of the sterile gloves (6).

5. The plant tissue culture sterile operating box according to claim 1, wherein: The outside of the box (1) is fixedly connected with a controller (7), the outside of the box (1) is fixedly connected with a butt plate (9), one end of the butt plate (9) is fixedly installed with an ultraviolet lamp (10), the ultraviolet lamp (10) is arranged in the inside of the box (1), and the ultraviolet lamp (10) is located at the top of the culture plate (12); the other end of the ultraviolet lamp (10) is electrically connected with the controller (7) through a wire.

6. The plant tissue culture sterile operating box according to claim 1, wherein: The bottom of the box (1) is fixedly connected with side plates (2), the bottom of the two side plates (2) is fixedly connected with movable wheels (3), and the tail of the four movable wheels (3) is provided with a locking structure.

7. The plant tissue culture sterile operating box according to claim 1, wherein: The front side of the box (1) is provided with a hinged door. The box (1) is transparent, both sides of the end of the box (1) are provided with operation holes (4), the inside of the two operation holes (4) is fixedly connected with sealing rings (5), the inside of the sealing ring (5) is fixedly connected with sterile gloves (6); the inside of the box (1) is fixedly connected with a support seat (8), the top end of the support seat (8) is fixedly installed with a culture plate (12), the middle part of the culture plate (12) is fixedly installed with a plurality of groups of culture dishes (13), both sides of the culture plate (12) are movably provided with clamping plates (11), the two clamping plates (11) are arranged at the bottom of the sterile gloves (6), and the inside of the clamping plate (11) is provided with a heat conduction structure.

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