Fluorescent peptide bond structure water treatment agent, preparation method and application

Abstract

The application discloses a preparation method and application of a fluorescent peptide bond structure water treatment agent, and a chemical structure thereof is shown in the following formula: wherein A represents an unmodified structure unit, a polymerization degree of which is n; B represents a modified structure unit, a polymerization degree of which is m; n+m represents a total polymerization degree; and m / (n+m) * 100% = 40% to 70%. The polyaspartic acid derivative prepared by the application can not only effectively chelate and coordinate with metal ions in circulating water, but also can form a protective film on the surface of carbon steel, so that the corrosion of carbon steel and other metals by atmosphere and harmful substances is reduced, and the efficient promotion of the scale inhibition performance and corrosion inhibition performance of polyaspartic acid is realized. In addition, the water treatment agent can realize online detection of different dosages and concentrations according to the fluorescence intensity, so that the best performance of the agent is exerted, the concentration multiple of the circulating water is improved, water resources are saved, and the purpose of energy saving and emission reduction is achieved.

Description

Technical Field

[0001] This invention belongs to the field of industrial circulating water treatment technology, specifically relating to a fluorescent peptide bond structure water treatment agent, its preparation method, and its application. Background Technology

[0002] In industrial circulating cooling water systems, the concentration of inorganic salts and metal ions continuously increases due to water evaporation and recycling, leading to corrosion of the pipe walls and significant scale buildup. This adverse effect can cause substantial economic losses and catastrophic accidents in industrial production. Therefore, adding water treatment agents is one of the most effective methods to solve this problem. However, traditional carboxylic acid and phosphate water treatment agents are difficult to biodegrade naturally, causing negative environmental impacts. Currently, the research and application of biodegradable water treatment agents is a hot topic and a future development direction in the water treatment field. Polyaspartic acid (PASP) is a recognized green water treatment agent. Its molecular structure contains peptide bonds, allowing it to be degraded by microorganisms into substances such as water and carbon dioxide. However, its chelating and dispersing abilities are currently inferior to traditional water treatment agents, and its scale inhibition and corrosion inhibition capabilities do not yet meet industrial needs. Furthermore, it does not possess fluorescent properties. Many researchers are currently improving its performance through chemical modification; however, simultaneously improving its scale inhibition and corrosion inhibition properties is challenging, and PASP itself does not exhibit fluorescent properties. Therefore, how to simultaneously improve the scale inhibition and corrosion inhibition performance of PASP through modification is a current research focus.

[0003] Based on this, the present invention proposes to graft a semi-EDTA structure and a fluorescent phenyl group onto the side chain of PASP molecules through chemical modification. This not only increases the content of carboxyl functional groups in the PASP molecule structure, but also adds a large number of heteroatoms (O, N) and aromatic ring structures. This not only improves its scale and corrosion inhibition performance, but also enables online detection of dosage concentration based on fluorescence intensity. Therefore, this application is of great significance in exploring a green scale and corrosion inhibitor with fluorescent tracer function and good solubility. Summary of the Invention

[0004] To address the shortcomings of existing technologies, the present invention aims to provide a method for preparing and applying a fluorescent peptide-structured water treatment agent. The polyaspartic acid derivative provided by this invention contains effective functional groups such as carboxyl, amide, and phenyl groups, and can simultaneously possess excellent scale inhibition and corrosion inhibition properties.

[0005] To achieve the above objectives, the present invention adopts the following technical solution:

[0006] In a first aspect, the present invention provides a fluorescent peptide bond structure water treatment agent (PASP-MA), the structural formula of which is shown below:

[0007]

[0008] Where A represents an unmodified structural unit with a degree of aggregation of n, and B represents a modified structural unit with a degree of aggregation of m. n+m represents the total degree of aggregation, and m / (n+m)×100%=40%~70%.

[0009] This invention uses polysuccinimide (PSI), N , N Using dimethylformamide (DMF), m-phenylenediamine (MXDA), bromoacetic acid (BA), and sodium carbonate (Na₂CO₃) as the main raw materials, the PASP intermediate is first obtained by ring-opening grafting of m-phenylenediamine onto polysuccinimide. Then, under alkaline conditions, the PASP intermediate is reacted with bromoacetic acid (BA) to obtain the target product PASP-MA. The specific reaction route is shown in the following equation:

[0010]

[0011] Includes the following steps:

[0012] Dissolve polysuccinimide in N , N Add m-phenylenediamine and distilled water to dimethylformamide, adjust the pH to 9-11, and continue the reaction in a water bath at 40 ℃-70 ℃ for 20-30 h to obtain the PASP intermediate product after purification.

[0013] The PASP intermediate was completely dissolved in distilled water, and bromoacetic acid aqueous solution was added first, followed by sodium carbonate aqueous solution. The reaction was carried out at 40 ℃~70 ℃ for 20~30 h. The target product, fluorescent peptide bond structure water treatment agent PASP-MA, was obtained by separation and purification.

[0014] Specifically, the molar ratio of polysuccinimide to m-phenylenediamine can be 0.1:(0.1~5). Use 1-3 mL of polysuccinimide per 5 mmol. N , N Dissolve dimethylformamide and add 3-10 mL of distilled water for every 5 mmol of polysuccinimide.

[0015] Specifically, the molar ratio of PASP intermediate to bromoacetic acid and sodium carbonate can be 0.1:(0.1~5):(0.5~1.5).

[0016] Furthermore, the pH can be adjusted to 9-11 using a buffer solution of sodium bicarbonate and sodium carbonate. More preferably, the molar ratio of sodium bicarbonate to sodium carbonate is 1:1.

[0017] Thirdly, the present invention also provides the application of the fluorescent peptide bond structure water treatment agent as a scale inhibitor or corrosion inhibitor in circulating cooling water.

[0018] This invention modifies polysuccinimide via a ring-opening reaction to prepare a multifunctional polyaspartic acid derivative with fluorescent properties, which can simultaneously and efficiently improve the scale inhibition and corrosion inhibition performance of industrial circulating water. Compared with the prior art, the beneficial effects of this invention are as follows:

[0019] 1) This invention introduces a fluorescent chromophore, phenyl, and a semi-EDTA structure into the side chain of polyaspartic acid. This structure can effectively chelate and coordinate with metal ions in circulating water, improving the scale inhibition performance of polyaspartic acid. Secondly, the phenyl-containing semi-EDTA structure can adsorb onto the metal surface to form a thin film, protecting carbon steel and other metals from atmospheric and harmful substances corrosion, thus achieving a high-efficiency improvement in the scale inhibition and corrosion inhibition performance of polyaspartic acid. This type of water treatment agent can also achieve online detection of dosage and concentration based on fluorescence intensity, maximizing agent performance, increasing the concentration ratio of circulating water, saving water resources, and achieving energy conservation and emission reduction goals, showing promising application prospects.

[0020] 2) The synthesis steps of this invention are simple and green, and the synthesized product is a new type of green phosphorus-free water treatment agent. It has scale inhibition, corrosion inhibition and fluorescence properties, and is easily degraded by microorganisms, with good biological and environmental compatibility. Attached Figure Description

[0021] Figure 1 The PASP prepared in Example 3 of this invention 1 H NMR spectrum;

[0022] Figure 2 The PASP-MA prepared in Example 3 of this invention 1 H NMR spectrum;

[0023] Figure 3 The excitation and emission spectra of the PASP-MA prepared in Example 4 of this invention are shown.

[0024] Figure 4 The fluorescence spectra of PASP-MA aqueous solutions of different concentrations prepared in Example 4 of this invention are shown.

[0025] Figure 5 This is a linear relationship graph between the fluorescence intensity of PASP-MA prepared in Example 4 of the present invention and its corresponding concentration. Detailed Implementation

[0026] The technical solutions of the present invention will be clearly and completely described below with reference to the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of the present invention.

[0027] In the following embodiments, unless otherwise specified, all raw materials used are ordinary commercially available products that can be directly purchased or can be prepared using conventional methods in the art.

[0028] The polysuccinimide used in the following examples has a molecular weight of 7000-8000 and was purchased from Shanghai Maclean Biochemical Technology Co., Ltd.

[0029] Example 1

[0030] (1) Weigh 5 mmol of polysuccinimide (PSI) into a reaction vessel and add 1 mL of PSI. N , N Dissolve dimethylformamide (DMF), add 5 mmol of m-phenylenediamine (MXDA) and 9 mL of H2O, and add a prepared sodium bicarbonate and sodium carbonate buffer solution (sodium bicarbonate and sodium carbonate molar ratio of 1:1, the same below) to maintain the pH at 9-11. Heat the mixture in a water bath at 50 °C for 24 h. Transfer the resulting solution to a dialysis bag with a molecular weight cutoff of 1000, dialyze it in distilled water for 3 days, and evaporate the retentate to dryness using a rotary evaporator to obtain the PASP intermediate.

[0031] (2) Weigh 1.278 mmol acetic acid (BA), 4.26 mmol sodium carbonate, and 0.426 mmol PASP intermediate, and add 5 mL of distilled water to dissolve them completely. Add the PASP intermediate aqueous solution and bromoacetic acid aqueous solution to the reaction vessel first, then slowly add the sodium carbonate aqueous solution dropwise. Heat the reaction vessel in a 50 °C water bath for 24 h. Transfer the resulting solution to a dialysis bag with a molecular weight cutoff of 1000, dialyze it in distilled water for 2 days, and evaporate the retentate to dryness using a rotary evaporator to obtain the target product PASP-MA.

[0032] Example 2

[0033] (1) Weigh 5 mmol of PSI and place it in a reaction vessel. Add 1 mL of DMF to dissolve it, then add 5 mmol of MXDA and 9 mL of H2O. Add the prepared sodium bicarbonate and sodium carbonate buffer solution to maintain the pH at 9-11. Heat the reaction in a water bath at 60 °C for 24 h. Transfer the resulting solution into a dialysis bag with a molecular weight cutoff of 1000 and dialyze it in distilled water for 3 days. Use a rotary evaporator to evaporate the retentate to dryness, thus obtaining the PASP intermediate.

[0034] (2) Weigh 1.704 mmol of bromoacetic acid, 5.112 mmol of sodium carbonate, and 0.426 mmol of PASP intermediate, and add 5 mL of distilled water to dissolve them completely. Add the aqueous solutions of PASP intermediate and bromoacetic acid to the reaction vessel first, then slowly add the aqueous solution of sodium carbonate. Heat the reaction vessel in a 45 °C water bath for 24 h. Transfer the resulting solution to a dialysis bag with a molecular weight cutoff of 1000, dialyze it in distilled water for 2 days, and evaporate the retentate to dryness using a rotary evaporator to obtain the target product PASP-MA.

[0035] Example 3

[0036] (1) Weigh 10 mmol of PSI and place it in a reaction vessel. Add 2 mL of DMF to dissolve it, then add 50 mmol of MXDA and 8 mL of H2O. Add the prepared sodium bicarbonate and sodium carbonate buffer solution to maintain the pH at 9-11. Heat in a water bath at 50 °C for 24 h. Transfer the resulting solution into a dialysis bag with a molecular weight cutoff of 1000 and dialyze it in distilled water for 3 days. Use a rotary evaporator to evaporate the retentate to dryness, thus obtaining the PASP intermediate.

[0037] (2) Weigh 1.278 mmol of bromoacetic acid, 4.26 mmol of sodium carbonate, and 0.426 mmol of PASP intermediate, and add 5 mL of distilled water to dissolve them completely. Add the aqueous solutions of PASP intermediate and bromoacetic acid to the reaction vessel first, then slowly add the aqueous solution of sodium carbonate. Heat the reaction vessel in a 45 °C water bath for 24 h. Transfer the resulting solution to a dialysis bag with a molecular weight cutoff of 1000, dialyze it in distilled water for 2 days, and evaporate the retentate to dryness using a rotary evaporator to obtain the target product PASP-MA.

[0038] Figure 1 For PASP 1 The H NMR spectrum shows two broad peaks at 4.5 ppm and 2.78 ppm, which correspond to the hydrogen atoms of -CH- and -CH2- in the PASP structure, respectively. Figure 2 For the target product PASP-MA 1 The ¹H NMR spectrum showed two broad peaks at 4.34 ppm and 2.73 ppm, with chemical shifts similar to those of PASP. Simultaneously, two novel peaks at 3.71 ppm and 7.37 ppm corresponded to the -CH₂- peaks on the side chain and the phenyl group in the PASP-MA product. This confirms the successful preparation of PASP-MA. Figure 2Based on the peak area calculation of -CH2- on the phenyl group and -CH2- in the side chain structure of PASP-MA, the m / (n+m)×100% of the target product PASP-MA is 45.5%.

[0039] Based on the same characterization method, the m / (n+m)×100% values ​​of the target product PASP-MA obtained in Example 1 and Example 2 were calculated to be 50.5% and 51.3%, respectively.

[0040] Example 4

[0041] (1) Weigh 10 mmol of PSI and place it in a reaction vessel. Add 3 mL of DMF to dissolve it, then add 50 mmol of MXDA and 8 mL of H2O. Add a buffer solution of sodium bicarbonate and sodium carbonate to maintain the pH at 9-11. Heat in a water bath at 50 °C for 24 h. Transfer the resulting solution into a dialysis bag with a molecular weight cutoff of 1000 and dialyze it in distilled water for 3 days. Use a rotary evaporator to evaporate the retentate to dryness, thus obtaining the PASP intermediate.

[0042] (2) Weigh 1.704 mmol of bromoacetic acid, 5.112 mmol of sodium carbonate, and 0.426 mmol of PASP intermediate, and add 5 mL of distilled water to dissolve them completely. First, add the aqueous solution of PASP intermediate and the aqueous solution of bromoacetic acid to the reaction vessel, then slowly add the aqueous solution of sodium carbonate. The reaction vessel is placed in a 45 ℃ water bath and heated for 24 h. The resulting solution is placed in a dialysis bag with a molecular weight cutoff of 1000, dialyzed in distilled water for 2 days, and the retentate is evaporated to dryness using a rotary evaporator to obtain the target product PASP-MA. The percentage of the target product PASP-MA synthesized is m / (n+m)×100%=46.1%.

[0043] Calcium carbonate scale inhibition performance test: Static scale inhibition method

[0044] The performance of PASP-MA prepared in Examples 1-4 in inhibiting calcium carbonate scale was tested using the following methods:

[0045] According to the national standard GB / T 16632-2019, the performance of the scale inhibitor was determined using the static scale inhibition method. In the calcium carbonate inhibition test: the concentration of calcium chloride (CaCl2) solution was 0.15 mol / L, the concentration of sodium bicarbonate (NaHCO3) solution was 0.3 mol / L, and the concentration of borax solution was 0.01 mol / L.

[0046] Solution preparation:

[0047] 1. Blank sample: Add 220 mL of water, 10 mL of CaCl2 solution, 10 mL of borax solution, and 10 mL of NaHCO3 solution to a 250 mL Erlenmeyer flask with a stopper in sequence. (One set each at room temperature and heating).

[0048] 2. Sample:

[0049] 1) Preparation method for a sample concentration of 10 mg / L: Add 217.5 mL of water, 10 mL of CaCl2 solution, 10 mL of borax solution, 2.5 mg / mL PASP-MA, and 10 mL of NaHCO3 solution to a 250 mL Erlenmeyer flask with a stopper in sequence.

[0050] 2) Preparation method for a sample concentration of 20 mg / L: Add 215 mL of water, 10 mL of CaCl2 solution, 10 mL of borax solution, 5 mg / mL PASP-MA, and 10 mL of NaHCO3 solution to a 250 mL Erlenmeyer flask with a stopper in sequence.

[0051] 3) Preparation method for a sample concentration of 30 mg / L: Add 212.5 mL of water, 10 mL of CaCl2 solution, 10 mL of borax solution, 7.5 mg / mL PASP-MA, and 10 mL of NaHCO3 solution to a 250 mL Erlenmeyer flask with a stopper in sequence.

[0052] The sample was heated in an 80 ℃ water bath for 10 h. After heating, it was cooled to room temperature and filtered. The supernatant of the blank sample and the sample solution was determined three times by EDTA titration. The calcium carbonate scale inhibition performance is shown in Tables 1 and 2. The calcium carbonate scale inhibition efficiency was calculated according to formula (1):

[0053]

[0054] In the formula:

[0055] V2—The volume of supernatant consumed in the EDTA titration of the sample;

[0056] V1 — The volume of supernatant consumed in the heating group during EDTA titration of blank sample;

[0057] V0 — The volume of supernatant consumed in the EDTA titration of the blank sample at room temperature.

[0058] Table 1. Calcium carbonate scale inhibition efficiency in Examples 1 and 2

[0059]

[0060] Table 2. Calcium carbonate scale inhibition efficiency in Examples 3 and 4

[0061]

[0062] As shown in Tables 1 and 2, the PASP-MA provided by the present invention has good inhibition performance on calcium carbonate scale. In Example 4, the scale inhibition efficiency can reach 99.15% when the agent concentration is 30 mg / L.

[0063] Corrosion inhibition performance test: Rotary plate method

[0064] Test Examples 3-4: PASP-MA pairs prepared in 20 # The corrosion inhibition performance of carbon steel is tested using the following methods:

[0065] According to GB / T1875—2014, for the preparation of standard prepared water, weigh 7.35 g of calcium chloride dihydrate, 4.93 g of magnesium sulfate heptahydrate, and 6.58 g of sodium chloride and dissolve them completely in approximately 7 L of water; separately weigh 1.68 g of sodium bicarbonate and dissolve it completely in approximately 1 L of water. Mix the two solutions thoroughly and dilute with water to 10 L. The concentrations of calcium ions, magnesium ions, chloride ions, and bicarbonate ions in this standard prepared water are 200.4 mg / L, 48.62 mg / L, 399.1 mg / L, and 122.0 mg / L.

[0066] Experimental steps:

[0067] 1) Pretreatment of test pieces: Use filter paper to clean 20 mm of the test piece. # Wipe the carbon steel test pieces clean with anti-rust grease, then wipe them separately with degreased cotton in anhydrous ethanol. Use no less than 50 mL of the above reagent for every ten test pieces. After wiping clean, blot dry with filter paper, place in a desiccator for 4 h, weigh (accurate to 0.2 mg), record as m1, and store in a desiccator for later use.

[0068] 2) Prepare a 15 mg / L PASP-MA solution in a 2000 mL test cup using standard prepared water. This is the test solution.

[0069] 3) Hanging the test piece and rotation test: When the test solution reaches 45 ℃, hang the test piece, start the rotation system, and make the test piece rotate at a speed of 95 r / min. The test starts and the duration is 72 h.

[0070] 4) Replenishing the test solution: Add test water as needed according to the test results to keep the liquid level at the graduation mark.

[0071] 5) Stop the rotation test: When the specified time is reached, stop the rotation test, take out the test piece and observe and record its appearance.

[0072] 6) Post-treatment of carbon steel test pieces: Clean the test pieces with a brush, then rinse them in the pickling solution for approximately 30 seconds. Remove them, rinse quickly with tap water, and immediately immerse them in sodium hydroxide solution for about 30 seconds. Remove them, rinse with water, wipe with filter paper and blot dry, soak in anhydrous ethanol for about 3 minutes, place them on clean filter paper, blot dry with filter paper, and place them in a desiccator for at least 4 hours. Weigh the sample (accurate to 0.2 mg) and record the weight as m2. Simultaneously perform a blank pickling test on the test pieces to calibrate the pickling process.

[0073] 7) Alkaline washing solution: 60 g / L sodium hydroxide solution. Acid washing solution: 8 g of hexamethylenetetramine is added to 1000 mL of (1+4) hydrochloric acid solution (i.e., 37% concentrated hydrochloric acid and water are mixed at a volume ratio of 1:4), dissolved, and then mixed well.

[0074] Blank test: Perform a blank test without adding water treatment agent.

[0075] Result calculation.

[0076] Table 3

[0077] m1 — Mass of the sample before pretreatment, in grams (g);

[0078] m2 — the mass of the sample after post-processing, in grams (g);

[0079] m — the numerical value of the mass loss of the test piece, in grams (g).

[0080] The corrosion rate is represented by v, with units of mm / a, and is calculated according to formula (2):

[0081]

[0082] In the formula:

[0083] m — numerical value of mass loss of the test piece, in grams (g);

[0084] m0—The average mass loss of the sample in the blank pickling test, in grams (g);

[0085] s — The numerical value of the surface area of ​​the test piece, in square centimeters (cm²). 2 );

[0086] p – The density of the sample, expressed in grams per cubic centimeter (g / cm³). 3 )

[0087] t — The numerical value of the test time, in hours (h);

[0088] 8760 – Hours equivalent to a year, expressed in hours per year (h / a);

[0089] 10 – The number of millimeters equivalent to 1 cm, expressed in millimeters per centimeter (mm / cm).

[0090] The corrosion inhibition rate η is calculated according to formula (3):

[0091]

[0092] In the formula:

[0093] V0 — The numerical value of the corrosion rate of the blank test specimen, in millimeters per year (mm / a);

[0094] V1 – The corrosion rate of the test piece, expressed in millimeters per year (mm / a).

[0095] Table 3. Determination of corrosion inhibition rate

[0096]

[0097] As shown in Table 3, the PASP-MA provided by this invention is compatible with 20... # Carbon steel exhibits good corrosion inhibition properties. Under optimal conditions, a corrosion inhibitor concentration of 15 mg / L can achieve a corrosion inhibition efficiency of 72%.

[0098] It should be noted that all the above embodiments belong to the same inventive concept, and the descriptions of each embodiment have different focuses. Where the description in a particular embodiment is not detailed, please refer to the description in other embodiments.

[0099] Determination of the fluorescence properties of PASP-MA:

[0100] Figure 3 The figure shows the excitation and emission spectra of the PASP-MA obtained in Example 4. As can be seen from the figure, the excitation wavelength and emission wavelength of PASP-MA are 355 nm and 443 nm, respectively. The emission wavelength is greater than the excitation wavelength. This is because during the transition of excited-state electrons to the ground state, the accompanying non-radiative transition consumes some energy, causing a certain shift in the fluorescence characteristic wavelength. This phenomenon is called Stokes shift.

[0101] Figure 4 The figures show the fluorescence spectra of PASP-MA aqueous solutions at different concentrations. As can be seen from the figures, the fluorescence intensity of PASP-MA gradually increases with increasing concentration (20–100 ppm) within a certain range. For each increase in the same concentration gradient, the increase in PASP-MA fluorescence intensity at the same wavelength peak is basically consistent.

[0102] Figure 5The figure shows the linear relationship between the fluorescence intensity of PASP-MA and its corresponding concentration. As can be seen from the figure, within the concentration range of 20–100 ppm, there is a good linear correlation between the fluorescence intensity of PASP-MA and its corresponding concentration, with a linear correlation coefficient R0. 2 =0.99825, which lays the foundation for realizing online industrial monitoring and automatic dosing.

[0103] The embodiments described above are merely illustrative of implementation methods of the present invention, and while the descriptions are specific and detailed, they should not be construed as limiting the scope of the invention patent. It should be noted that those skilled in the art can make various modifications and improvements without departing from the concept of the present invention, and these all fall within the protection scope of the present invention. Therefore, the protection scope of this invention patent should be determined by the appended claims.

Claims

1. A fluorescent peptide bond structure water treatment agent, characterized in that, The structural formula is as follows: ； Where A represents an unmodified structural unit with a degree of aggregation of n, B represents a modified structural unit with a degree of aggregation of m, n+m represents the total degree of aggregation, and m / (n+m)×100%=40%~70%; When the concentration of the fluorescent peptide bond structure water treatment agent is 30 mg / L, it achieves a scale inhibition efficiency of 99.15%. The concentration of the fluorescent peptide bond structure water treatment agent is 15 mg / L, effective against 20... # Carbon steel achieves a corrosion inhibition efficiency of 72%; The fluorescent peptide bond structure water treatment agent exhibits fluorescence properties, and its fluorescence intensity shows a good linear correlation with the corresponding concentration, with a linear correlation coefficient R. 2 =0.99825, used for industrial online monitoring and automatic dosing.

2. The preparation method of the fluorescent peptide bond structure water treatment agent according to claim 1, characterized in that, Includes the following steps: Dissolve polysuccinimide in N , N Add m-phenylenediamine and distilled water to dimethylformamide, adjust the pH to 9-11, react at 40 ℃-70 ℃ for 20-30 h, and purify to obtain PASP intermediate product; The PASP intermediate was completely dissolved in distilled water, and bromoacetic acid aqueous solution was added first, followed by sodium carbonate aqueous solution. The reaction was carried out at 40℃~70℃ for 20~30 h. The target product, a fluorescent peptide bond structure water treatment agent, was obtained by separation and purification.

3. The preparation method of the fluorescent peptide bond structure water treatment agent as described in claim 2, characterized in that, The molar ratio of polysuccinimide to m-phenylenediamine is 0.1:(0.1~5).

4. The preparation method of the fluorescent peptide bond structure water treatment agent as described in claim 2, characterized in that, The molar ratio of PASP intermediate to bromoacetic acid and sodium carbonate is 0.1:(0.1~5):(0.5~1.5).

5. The preparation method of the fluorescent peptide bond structure water treatment agent as described in claim 2, characterized in that, Adjust the pH to 9-11 using a buffer solution of sodium bicarbonate and sodium carbonate.

6. The preparation method of the fluorescent peptide bond structure water treatment agent as described in claim 5, characterized in that, The molar ratio of sodium bicarbonate to sodium carbonate is 1:

1.

7. The application of the fluorescent peptide bond structure water treatment agent according to claim 1 as a scale inhibitor, corrosion inhibitor, or in online monitoring and automatic dosing of circulating cooling water.

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