Toothpaste with efficacy of preventing and treating oral ulcers and preparation method thereof

Abstract

The present invention belongs to the technical field of oral care. More specifically, it relates to a toothpaste having the efficacy of preventing and treating oral ulcers and a preparation method thereof. The toothpaste comprises the following components by mass fraction: 0.1-3% of royal jelly enzymatic hydrolysate, 20-25% of abrasive, 40-55% of moisturizer, 0.1-3% of thickener, 2.0-3.0% of foaming agent, 0.1-0.3% of preservative, 0.1-0.5% of colorant, and 10-45% of deionized water. The toothpaste of the present invention has a good therapeutic effect on recurrent oral ulcers.

Description

Technical Field

[0001] The present invention belongs to the technical field of oral care, and more specifically, relates to a toothpaste having the efficacy of preventing and treating oral ulcers and a preparation method thereof. Background Art

[0002] Recurrent aphthous ulcer (RAU), also known as recurrent oral ulcer, is the most common oral mucosal ulcer disease. In a specific population, the prevalence of RAU can be as high as 50%, and the incidence in women is generally higher than that in men. The characteristics of the disease are obvious pain during the onset period, and it is cyclical, recurrent, and self-limiting. The lesions appear as isolated, round or oval superficial ulcers, showing "yellow, red, concave, and painful", that is, the surface is covered with a yellow pseudomembrane, surrounded by a red halo, with a central depression, and obvious pain. Treatment is mainly local, and severe cases require systemic treatment. [1] .

[0003] Royal jelly is a milky white colloid secreted by the pharyngeal glands of worker bees. Studies have shown that royal jelly has anti-aging, anti-tumor, antibacterial and immune-regulating effects, but the protein in royal jelly is very unstable. The enzymatic polypeptide obtained after moderate hydrolysis is highly stable, and its solubility, hygroscopicity and other properties are improved. For example, Qi Xiangyang et al. used 4 different proteases (protease P, protease N, protease M, composite protease) to hydrolyze royal jelly. The results showed that different proteases had different hydrolysis effects, among which the composite plant protein enzyme had a better hydrolysis effect, and its hydrolysate was better in water solubility and heat and acid stability than fresh royal jelly. [2] . Moreover, the royal jelly products after enzymatic hydrolysis show many new characteristics compared with royal jelly: For example, Toshiro Matsui et al. used pepsin, chymotrypsin and trypsin to hydrolyze royal jelly, and the prepared enzymatic hydrolysate had an IC50 of 0.099 mg / mL for ACE, showing a high blood pressure lowering activity, while fresh royal jelly and royal jelly protein components did not show ACE inhibitory activity. Feeding the enzymatic hydrolysate to spontaneously hypertensive rats found that their cardiac systolic pressure was significantly reduced. [3] ; Ji Wenjing's research found that royal jelly enzymatic hydrolysate has certain anti-aging and immunity-enhancing effects. Under optimized trypsin hydrolysis conditions, low doses of royal jelly enzymatic hydrolysate can intervene in the weight loss of mice induced by D-galactose, and high doses can improve the decreased activity of anti-aging mice. At the same time, compared with aging mice, low doses of royal jelly enzymatic hydrolysate showed a significant effect on promoting the transformation of splenic B lymphocytes in mice stimulated by lipopolysaccharide, and the high dose effect was more significant, and the difference was significant compared with the original royal jelly. [4] .

[0004] However, the use of royal jelly enzymatic hydrolysate in promoting the proliferation of oral mucosal epithelial cells, repairing oral mucosa and treating oral ulcers has not been found so far.

[0005] [1] Oral Mucosal Diseases Committee of the Chinese Stomatological Association, Chinese Stomatological Association Traditional Chinese and Western Medicine Integration Committee. Guidelines for the diagnosis and treatment of recurrent aphthous ulcers (trial version) [J]. Chinese Journal of Stomatology, 2012, 47(7): 402-404.

[0006] [2] Qi Xiangyang, Optimization of conditions for enzymatic preparation of water-soluble royal jelly and study of its characteristics [J]. Journal of Chinese Institute of Food Science and Technology, 2009, 9(4): 155-159.

[0007] [3] Matsui T, YuyoshiA, DOI S, et al. Gastrointestinal enzyme production of bioactive peptides from royal jelly protein and their antihypertensiveability in SHR[J]. The Journal of Nutritional Biochemistry, 2002, 13(2): 80-86.

[0008] [4] Ji Wenjing. Study on the physicochemical properties, biological activity and mechanism of action of royal jelly enzymatic hydrolysate[D]. Hangzhou: Zhejiang University, 2013. Summary of the invention

[0009] The present invention first discovered that the product with a molecular weight between 3 and 5 kDa separated from the royal jelly hydrolyzate under specific conditions showed a significant effect of promoting the proliferation and migration of oral mucosal epithelial cells within a certain concentration range. This suggests that royal jelly hydrolyzate may have the effect of repairing oral mucosa, and this part of the discovery has been applied for on the same day. The inventor confirmed through further experiments that toothpaste containing the royal jelly hydrolyzate has the effect of preventing and treating recurrent oral ulcers, and thus made this application.

[0010] Therefore, the object of the present invention is to provide a toothpaste having the efficacy of preventing and treating oral ulcers.

[0011] Another object of the present invention is to provide a method for preparing the toothpaste having the efficacy of preventing and treating oral ulcers.

[0012] The above-mentioned purpose of the present invention is achieved through the following technical solutions:

[0013] The invention provides a toothpaste with the efficacy of preventing and treating oral ulcers. The toothpaste comprises the following components in mass fractions: 0.1-3% of royal jelly enzymatic hydrolysate, 20-25% of abrasive, 40-55% of moisturizer, 0.1-3% of thickener, 2.0-3.0% of foaming agent, 0.1-0.3% of preservative, 0.1-0.5% of colorant and 10-45% of deionized water.

[0014] Furthermore, the royal jelly enzymatic hydrolysate is a product obtained by hydrolysis of bromelain and proteinase K. The enzymatic hydrolysates obtained by different enzyme types show great differences in physiological activity. The inventors compared the effects of three complex enzyme combinations on the proliferation and migration of human oral mucosal epithelial cells and found that papain + proteinase K enzymatic hydrolysate did not show the same proliferative activity in each molecular weight segment. Secondly, the proliferative activity of bromelain + pepsin enzymatic hydrolysate was significantly lower than that of bromelain + proteinase K combination.

[0015] Furthermore, the molecular weight of the royal jelly enzymatic hydrolysis product is less than 5kDa.

[0016] Furthermore, the molecular weight of the royal jelly enzymatic hydrolysis product is 3 to 5 kDa.

[0017] Secondly, the molecular weight of the enzymatic hydrolysis product also has a non-negligible effect on its activity. Bromelain + proteinase K hydrolysis products with different molecular weights show different promotion degrees. Among them, the proliferation-promoting activity of 3-5kDa bromelain + proteinase K royal jelly hydrolysis products is significantly greater than that of molecular weight <3kDa, and extremely significantly greater than that of molecular weight >5kDa, and the differences are statistically significant.

[0018] Furthermore, the royal jelly enzymatic hydrolysate is prepared by the following steps:

[0019] S1. Add royal jelly to deionized water at a ratio of 1:9-15, stir well, let stand at 4°C for 30-60 min, centrifuge at 3000-6000 g for 10-25 min, and take the supernatant;

[0020] S2. Add bromelain to the supernatant, mix thoroughly, and then enzymolyze for 2 to 6 hours; then add proteinase K, mix thoroughly, and then enzymolyze for 1 to 3 hours;

[0021] S3. After the enzymatic hydrolysis is completed, the product is inactivated and centrifuged. The supernatant is filtered using 5kDa and 3kDa ultrafiltration membranes in turn, and freeze-dried to obtain the royal jelly enzymatic hydrolysis product.

[0022] Furthermore, in step S2, the conditions for enzymatic hydrolysis of bromelain are: enzyme addition amount is 300-500 U / g, reaction temperature is 45-65° C., and pH is 6.0-8.0.

[0023] Furthermore, in step S2, the conditions for the enzymatic hydrolysis with proteinase K are: the enzyme addition amount is 300-500 U / g, the reaction temperature is 50-60° C., and the pH is 7.5-11.0.

[0024] Furthermore, the abrasive agent is silicon dioxide; the moisturizer is selected from one or more of PEG-400, sorbitol and glycerin; and the thickener is selected from one or more of sodium carboxymethyl cellulose, xanthan gum, guar gum and carrageenan.

[0025] Furthermore, the foaming agent is selected from one or more of sodium lauryl sulfate, sodium lauryl sulfate and cocoyl propyl betaine; the preservative is selected from one or more of benzoic acid and its salts, sorbic acid and its salts and parabens; and the colorant is titanium dioxide.

[0026] On the other hand, the present invention provides a method for preparing the toothpaste, comprising the following steps: accurately weighing each material, first dissolving the royal jelly hydrolysate uniformly with 1 / 3 of deionized water and setting aside; then adding a preservative and the remaining water into a stirring kettle, stirring evenly, adding a moisturizer, and stirring for 1 to 5 minutes; adding an abrasive, a colorant, a thickener, and a foaming agent into the stirring kettle, stirring for 10 to 30 minutes, adding the pre-dissolved royal jelly hydrolysate, continuing to stir for 5 to 10 minutes, and maintaining bubbling for 1 to 5 minutes when the vacuum degree reaches above -0.094 MPa, stopping stirring, discharging, and filling.

[0027] Application tests show that the ulcer area and pain value of patients who used toothpaste with royal jelly hydrolysate were significantly different from those who used toothpaste without royal jelly hydrolysate. The ulcer area and pain value were significantly reduced, and the healing rates on the 3rd day and 5+2 days increased by 20% and 63.34% respectively. This shows that toothpaste with royal jelly hydrolysate has a significant effect on recurrent oral ulcers.

[0028] The present invention has the following beneficial effects:

[0029] (1) The present invention first discovered that a product with a molecular weight between 3 and 5 kDa separated from royal jelly hydrolyzed under specific conditions has a significant effect on promoting the proliferation and migration of oral mucosal epithelial cells, which suggests that the product has a repairing effect on oral mucosal damage and can be used in the treatment of oral mucosal diseases.

[0030] (2) The present invention prepares a toothpaste containing the above royal jelly enzymatic hydrolysate, and application tests have shown that the toothpaste has a good therapeutic effect on recurrent oral ulcers. BRIEF DESCRIPTION OF THE DRAWINGS

[0031] Figure 1The results of Transwell migration experiment after treatment with royal jelly hydrolysate A2 at different concentrations;

[0032] in, Figure 1 ① is the control group, Figure 1 ②~④ are the treatment groups of 5mg / mL, 10mg / mL and 15mg / mL royal jelly enzymatic hydrolysate A2 respectively.

[0033] Figure 2 The statistical diagram of the number of cell migration of human oral mucosal epithelial cells after being treated with different concentrations of royal jelly enzymatic hydrolysate A2;

[0034] Compared with the control group, * P<0.05, ** P<0.01, *** P<0.001. DETAILED DESCRIPTION

[0035] The present invention can be further described by the following examples, however, the scope of the present invention is not limited to the following examples. It will be appreciated by those skilled in the art that various changes and modifications may be made to the present invention without departing from the spirit and scope of the present invention.

[0036] Preparation Example 1: Preparation of Royal Jelly Enzyme Hydrolysate

[0037] S1. Add royal jelly to deionized water at a ratio of 1:10, stir well, let stand at 4°C for 45 min, centrifuge at 4000 g for 10 min, and take the supernatant;

[0038] S2, adding 400 U / g of bromelain to the supernatant, mixing thoroughly, and performing enzymolysis at 50°C, pH 7.0 for 4 h; then adding 300 U / g of proteinase K, mixing thoroughly, and performing enzymolysis at 55°C, pH 7.5 for 2.5 h;

[0039] S3. After the enzymatic hydrolysis is completed, the enzyme is inactivated at 100°C for 5 minutes, the mixture is centrifuged, and the supernatant is filtered using 5kDa and 3kDa ultrafiltration membranes respectively. After freeze-drying, royal jelly enzymatic hydrolysis product A1 with a molecular weight of less than 3kDa, royal jelly enzymatic hydrolysis product A2 with a molecular weight of 3kDa to 5kDa, and royal jelly enzymatic hydrolysis product A3 with a molecular weight of more than 5kDa are obtained respectively.

[0040] Preparation Example 2 is different from Preparation Example 1 in that papain is used instead of bromelain, and the enzymolysis conditions are: enzyme addition amount is 400 U / g, temperature is 55° C., pH 7.5, and the other parameters are the same as those in Example 1, and royal jelly enzymolysis product B1 with a molecular weight of <3 kDa, royal jelly enzymolysis product B2 with a molecular weight of 3 kDa to 5 kDa, and royal jelly enzymolysis product B3 with a molecular weight of >5 kDa are obtained respectively.

[0041] Preparation Example 3 differs from Preparation Example 1 in that pepsin is used instead of proteinase K, and the enzymatic hydrolysis conditions are: enzyme addition amount is 300 U / g, temperature is 37°C, pH is 2.0, and the other parameters are the same as those in Example 1, and royal jelly enzymatic hydrolysis product C1 with a molecular weight of <3 kDa, royal jelly enzymatic hydrolysis product C2 with a molecular weight of 3 kDa to 5 kDa, and royal jelly enzymatic hydrolysis product C3 with a molecular weight >5 kDa are obtained respectively.

[0042] Test Example 1: Human oral mucosal epithelial cell proliferation test

[0043] Human oral mucosal epithelial cells in the third generation logarithmic growth phase were prepared with complete culture medium for human oral mucosal epithelial cells (purchased from Wuhan Pronocell Life Science Co., Ltd., catalog number CM-H203) to obtain a density of 1×10 6 100 μL of the cell suspension of 100 μg / mL was inoculated into a 96-well plate, with 8 wells in each group. After culturing for 24 h, the royal jelly hydrolysates A1-A3, B1-B3 and C1-C3 prepared above were respectively prepared into a test solution with a concentration of 10 mg / mL using complete culture medium, and 100 μL of the test solution was added into a 96-well plate at 37°C and 5% CO. 2 After culturing for 24 h under the conditions of 5% CO, the culture medium was discarded and CCK-8 solution was added to each well according to the instructions of the CCK-8 kit. 2 The cells were incubated for 4 h under the conditions of 490 nm and the absorbance (A) value was measured at a wavelength of 490 nm on an ELISA reader. This was repeated 3 times and the average value was taken to calculate the cell proliferation rate. The results are shown in Table 1 below.

[0044] Cell proliferation rate = (absorption value of experimental group - absorption value of blank control) / (absorption value of control group + absorption value of blank control) × 100%

[0045] Among them, the experimental group was cells treated with royal jelly enzymatic hydrolysate, the control group was untreated cells, and the blank control group was culture medium without cells.

[0046] Table 1. Effects of different royal jelly hydrolysates on the proliferation of human oral mucosal epithelial cells

[0047]

[0048]

[0049] Note: Compared with royal jelly enzymatic hydrolysate A2 group, * P<0.05, ** P<0.01, *** P<0.001.

[0050] When the cell proliferation rate is close to 100%, it means that the number of cells in the experimental group and the control group is equivalent; when the cell proliferation rate is less than 100%, it means that the number of cells in the experimental group is less than that in the control group; and when the cell proliferation rate is greater than 100%, it means that the number of cells in the experimental group is more than that in the control group.

[0051] As shown in Table 1 above, the royal jelly hydrolysates obtained by different complex enzyme combinations showed different effects on human oral mucosal epithelial cells, some of which showed a promoting effect, and some showed an inhibitory effect. This suggests that the type of enzymes and the size of the product molecular weight during the hydrolysis process play a decisive role in the physiological activity of the royal jelly hydrolysates. Among them, the products with a molecular weight of less than 5kDa obtained by hydrolysis with bromelain + proteinase K have a more obvious effect on the proliferation of human oral mucosal epithelial cells, and the royal jelly hydrolysate A2 with a molecular weight of 3-5kDa has the strongest effect on promoting the proliferation of human oral mucosal epithelial cells, with a proliferation rate of 175.94%, followed by the royal jelly hydrolysate A1 with a molecular weight of less than 3kDa, with a proliferation rate of 148.62%.

[0052] Experimental Example 2: Effects of different concentrations of royal jelly hydrolysate A2 on the proliferation of human oral mucosal epithelial cells

[0053] The royal jelly enzymatic hydrolysate A2 was prepared into test solutions of different concentrations using complete culture medium, and its effect on the proliferation activity of human oral mucosal epithelial cells was investigated according to the method of Experimental Example 1. The results are shown in Table 2 below.

[0054] Table 2 Effects of different concentrations of royal jelly hydrolysate A2 on the proliferation of human oral mucosal epithelial cells

[0055] Grouping Proliferation rate (%) 1mg / mL Royal Jelly Enzyme Hydrolysate A2 <![CDATA[118.46±3.81 ** ]]> 5mg / mL Royal Jelly Enzyme Hydrolysate A2 <![CDATA[156.33±10.54 * ]]> 10mg / mL Royal Jelly Enzyme Hydrolysate A2 182.31±12.06 15mg / mL Royal Jelly Enzyme Hydrolysate A2 <![CDATA[127.06±9.42 ** ]]> 20mg / mL Royal Jelly Enzyme Hydrolysate A2 <![CDATA[109.17±8.53 ** ]]>

[0056] Note: Compared with 10mg / mL royal jelly enzymatic hydrolysate A2, * P<0.05, ** P<0.01.

[0057] As can be seen from Table 2, royal jelly enzymatic hydrolysate A2 has a proliferation-promoting effect on human oral mucosal epithelial cells in the range of 1 to 20 mg / mL, and promotes the proliferation of oral mucosal epithelial cells in a dose-dependent manner in the range of 1 to 10 mg / mL, with the peak value appearing at 10 mg / mL, at which time the proliferation rate of human oral mucosal epithelial cells reaches 182.31%.

[0058] Experimental Example 3: Transwell Chamber Detection of Cell Migration

[0059] Human oral epithelial cells were resuspended in serum-free complete medium for human oral epithelial cells for 24 h, digested, washed twice with PBS, and resuspended in complete medium for oral epithelial cells to prepare a cell suspension. The cell density was adjusted to 5 × 10 5 Place the Transwell chamber in a 24-well plate, take 100 μL of cell suspension and add it to the Transwell chamber, add royal jelly enzymatic hydrolysate A2 to the chamber at a ratio of 1:1 between the test solution and the cell suspension, and incubate at 37°C and 5% CO 2 After culturing in a cell culture incubator for 24 hours, the Transwell chamber was taken out, the culture medium was removed, and the matrix gel and cells in the chamber were gently wiped with a cotton swab or cotton soaked in PBS; 600 μL of 4% paraformaldehyde fixative was added to a 24-well plate, the chamber was placed in the 24-well plate for 30 minutes, and the fixative was discarded. The plate was washed twice with PBS, and crystal violet working solution was added to the 24-well plate. The chamber was placed in the staining for 10 minutes, the chamber was taken out, and the inside and outside of the chamber were washed with PBS for 3 times. After air drying, the Transwell chamber was placed under a microscope for photography, and 3 to 5 fields of view were selected to count the number of migrated cells. The results are as follows Figure 1 shown.

[0060] from Figure 1 and Figure 2 It can be seen that compared with the control group, the number of cell migration was significantly increased after culture with the addition of royal jelly hydrolysate A2 at a concentration of 5-15 mg / mL, among which the promoting effect at concentrations of 5 mg / mL and 10 mg / mL was the most obvious, and there was a very significant difference compared with the control group.

[0061] Examples 1 to 3: A toothpaste for preventing and treating recurrent oral ulcers (mass fraction, %)

[0062]

[0063]

[0064] Preparation process:

[0065] Accurately weigh each material, first use 1 / 3 of deionized water to evenly dissolve the royal jelly hydrolysate, then add sodium benzoate and the remaining water into the stirring kettle, stir evenly, add PEG-400 and sorbitol, and stir for 3 minutes; add silicon dioxide, titanium dioxide, sodium carboxymethyl cellulose and sodium dodecyl sulfate into the stirring kettle, stir for 15 minutes, then add the pre-dissolved royal jelly hydrolysate, continue stirring for 5 minutes, keep bubbling for 3 minutes when the vacuum degree reaches above -0.094MPa, stop stirring, discharge the material, and fill it.

[0066] Test Example 1: The toothpaste prepared in Example 1 was tested for the following indicators according to conventional methods. The test results are shown in Table 3 below.

[0067] Table 3. Test results

[0068]

[0069] Test Case 2: Evaluation of the therapeutic effect on recurrent aphthous ulcer

[0070] 60 subjects were selected, aged 18 to 55 years old, regardless of gender, and without a history of allergies; they met the diagnostic criteria for recurrent aphthous ulcers; the ulcer occurred within 48 hours, and the pain VAS score was ≥3 points without treatment. The above subjects were randomly divided into 2 groups, 30 cases in each group, named as Example 1 and Control Group (the difference from Example 1 is that royal jelly enzymatic hydrolysate A2 is not added). Each group of subjects used the corresponding toothpaste for oral care every day, and took about 1 cm long toothpaste to brush their teeth every morning and evening, once in the morning and evening, and brushed their teeth for at least 3 minutes each time. The subjects were revisited on the 3rd day and 5+2 days of use, and the ulcer healing rates of the two groups of subjects were counted, and the changes in the ulcer status (ulcer pain value and ulcer area) before and after the test were recorded. The data were statistically analyzed using SPSS statistical software, and the results are shown in Tables 4 to 5 below.

[0071] The ulcer pain value is expressed by a digital pain scale (Visual Analog Scale, VAS), and the meaning of VAS is a 10 cm straight line. The 0 end of the straight line represents "no pain", and the 10 cm end represents "the most severe pain". Patients record the corresponding scale of the straight line according to the degree of pain they feel. The ulcer area refers to the product of the long diameter (mm) and the vertical diameter (mm) of the ulcer.

[0072] Table 4. Statistical results of ulcer healing rate

[0073] Trial Groups Healing rate on day 3 Healing rate on day 5+2 Embodiment 1 26.67％ 96.67％ Control group 6.67％ 33.33％

[0074] Table 5. Statistical results of ulcer area and pain value

[0075]

[0076] Note: Compared with the control group, ** P<0.01.

[0077] According to statistics, the ulcer areas of the patients in the embodiment group and the control group before the test were 9.31±1.26 mm 2 and 8.57±1.06mm 2Before the test, the pain values ​​of the two groups were 4.82±0.67 and 4.73±0.81, respectively. There was no significant difference between the two groups. As shown in Tables 4 and 5, there were significant differences in the ulcer area and pain values ​​of the two groups of patients in the same period. Compared with the control group, the ulcer area of ​​the first group of patients in the embodiment was significantly reduced, the pain value was significantly reduced, and the healing rates on the 3rd day and 5+2 days were increased by 20% and 63.34%, respectively. It shows that the toothpaste added with royal jelly enzymatic hydrolysate has a significant therapeutic effect on recurrent aphthous oral ulcers.

[0078] The above embodiments are preferred implementation modes of the present invention, but the implementation modes of the present invention are not limited to the above embodiments. Any other changes, modifications, substitutions, combinations, and simplifications that do not deviate from the spirit and principles of the present invention should be equivalent replacement methods and are included in the protection scope of the present invention.

Claims

1. A toothpaste having the efficacy of preventing and treating oral ulcers, characterized in that: The invention comprises the following components by mass fraction: 0.1-3% of royal jelly enzymatic hydrolysate, 20-25% of abrasive, 40-55% of moisturizing agent, 0.1-3% of thickener, 2.0-3.0% of foaming agent, 0.1-0.3% of preservative, 0.1-0.5% of colorant and 10-45% of deionized water; the molecular weight of the royal jelly enzymatic hydrolysate is 3-5 kDa, and the royal jelly enzymatic hydrolysate is prepared by the following steps: S1. Add royal jelly to deionized water at a ratio of 1:9-15, stir well, let stand at 4°C for 30-60 min, centrifuge at 3000-6000 g for 10-25 min, and take the supernatant; S2. Add bromelain to the supernatant, mix thoroughly, and then enzymolyze for 2 to 6 hours; then add proteinase K, mix thoroughly, and then enzymolyze for 1 to 3 hours; S3. After the enzymatic hydrolysis is completed, the product is inactivated and centrifuged. The supernatant is filtered using 5kDa and 3kDa ultrafiltration membranes in turn, and freeze-dried to obtain the royal jelly enzymatic hydrolysis product.

2. The toothpaste according to claim 1, characterized in that In step S2, the conditions for enzymatic hydrolysis of bromelain are: enzyme addition amount is 300-500 U / g, reaction temperature is 45-65° C., and pH is 6.0-8.

0.

3. The toothpaste according to claim 1, characterized in that In step S2, the conditions for the proteinase K enzymolysis are: the enzyme addition amount is 300-500 U / g, the reaction temperature is 50-60° C., and the pH is 7.5-11.

0.

4. The toothpaste according to claim 1, characterized in that The abrasive agent is silicon dioxide; the moisturizing agent is selected from one or more of PEG-400, sorbitol and glycerin; and the thickener is selected from one or more of sodium carboxymethyl cellulose, xanthan gum, guar gum and carrageenan.

5. The toothpaste according to claim 1 or 4, characterized in that: The foaming agent is selected from one or more of sodium lauryl sulfate, sodium lauryl sulfate and cocoyl propyl betaine; the preservative is selected from one or more of benzoic acid and its salts, sorbic acid and its salts and parabens; and the colorant is titanium dioxide.

6. A method for preparing the toothpaste according to claim 1, characterized in that: The method comprises the following steps: accurately weighing various materials, firstly using 1 / 3 of deionized water to uniformly dissolve royal jelly hydrolysate and setting aside; then adding preservative and remaining water into a stirring kettle, stirring evenly, adding moisturizer and stirring for 1-5 minutes; adding abrasive, colorant, thickener and foaming agent into the stirring kettle, stirring for 10-30 minutes, adding pre-dissolved royal jelly hydrolysate, continuing stirring for 5-10 minutes, maintaining bubbling for 1-5 minutes when the vacuum degree reaches above -0.094MPa, stopping stirring, discharging materials and filling to obtain the product.

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