Method for improving saline-alkali soil based on microbial inoculants

Abstract

The application belongs to the field of agricultural technology application, and particularly relates to a method for improving saline-alkali soil based on microbial agents. The method is realized through the following steps: firstly, deep ploughing and rotary tillage are performed on the saline-alkali soil, and then a composite microbial agent is applied on the surface of the saline-alkali soil; secondly, rotary tillage is performed on the saline-alkali soil, and after irrigation, salt-tolerant crops are selected for sowing. The improvement method provided by the application has a certain improvement effect on moderate to severe salinity, can effectively improve the physical and chemical properties of the soil in a short period of time, and increase the organic matter content in the soil. Meanwhile, after planting related crops, the survival rate of the crops can be improved, and the growth and development of the crops can be promoted. The composite microbial agent provided by the application uses mushroom residue as a carrier, can improve the adsorption and activation reproduction rate of the microbial agent in the saline-alkali soil, and can improve the improvement effect of the soil under the synergistic action of the strains, and can better adapt to the saline-alkali soil environment and improve the yield of crops.

Description

Technical Field

[0001] This invention belongs to the field of agricultural technology application, specifically relating to a method for improving saline-alkali soil based on microbial agents. Background Technology

[0002] Saline-alkali land, a typical form of soil degradation, has long plagued agricultural production and the ecological environment. Traditional remediation methods, such as physical improvement, water conservancy improvement, and chemical improvement, can alleviate salinization to some extent, but they are often costly, have limited effectiveness, and may cause secondary pollution. In recent years, with the continuous development of microbial technology, its application in saline-alkali land remediation has gradually demonstrated unique advantages.

[0003] Microbial inoculants are an important means of managing saline-alkali land. These inoculants typically contain a variety of beneficial microorganisms that work synergistically to improve the soil environment. By applying appropriate compound microbial inoculants, not only can the number of microorganisms in the soil be increased, enhancing soil biological activity, but nutrient cycling in the soil can also be promoted, improving crop resistance and yield. Therefore, establishing a sustainable ecological improvement technology that can improve soil texture, enhance soil nutrient availability, and simultaneously realize the resource utilization of plants in saline-alkali soil is crucial for the ecological environmental protection of saline-alkali soils. Summary of the Invention

[0004] To address the problems existing in the prior art, the present invention provides a method for improving saline-alkali soil based on microbial agents.

[0005] The technical solution adopted by the present invention to achieve the above objectives is as follows:

[0006] This invention provides a method for improving saline-alkali soil based on microbial inoculants, comprising the following steps:

[0007] (1) Deep plow and rotary till the saline-alkali land, and then spread compound microbial agents on the surface of the saline-alkali land;

[0008] (2) The saline-alkali land was rotary tilled again, and after irrigation, salt-tolerant crops were selected for sowing.

[0009] Furthermore, the compound microbial agent is composed of plant endophytic lysine-containing Bacillus, budding short-stem mold, Rhizobium freundii, and excipients.

[0010] Among the above-mentioned compound microbial agents, the viable counts of *Bacillus lysine-containing*, *Bacillus buddingus*, and *Rhizobium frutescens* were all greater than 10. 8 CFU / g.

[0011] Furthermore, the preservation number of the plant endophytic lysine bacillus is CGMCC NO.1.15291; the preservation number of the budding short-stem fungus is CGMCC NO.3.3984; and the preservation number of the Frederick rhizobium is CGMCC NO.1.4354. All three strains were purchased from the China General Microbiological Culture Collection Center.

[0012] Furthermore, the auxiliary material is composed of mushroom residue and calcium peroxide in a mass ratio of 1:0.15.

[0013] The auxiliary materials used in the preparation of the compound microbial agent of this invention are prepared by the following method: after the mushroom residue is sterilized at high temperature, the moisture content is adjusted to 35-40%, then zinc chloride and propylene glycol are added, stirred evenly, and allowed to stand for 15-18 hours. Then calcium peroxide is added and stirred evenly.

[0014] Furthermore, the amount of zinc chloride added accounts for 0.10-0.15% of the mass of the mushroom residue; the amount of propylene glycol added accounts for 5-6% of the mass of the mushroom residue.

[0015] The beneficial effects of this invention are as follows:

[0016] (1) The improved method provided by the present invention has a certain improvement effect on moderate to severe salinity and alkalinity. It can effectively improve the physical and chemical properties of the soil in a short period of time, increase the organic matter content in the soil, and at the same time, after planting related crops, it can improve the survival rate of crops and promote the growth and development of crops.

[0017] (2) The compound microbial agent provided by the present invention uses mushroom residue as a carrier, which can improve the adsorption and activation reproduction rate of the agent in saline-alkali soil. Under the synergistic effect of each strain, the soil improvement effect is improved, and it can better adapt to the saline-alkali soil environment and increase crop yield. Detailed Implementation

[0018] The technical solution of the present invention will be further explained and described below through specific embodiments.

[0019] The saline-alkali land experimental site used in this invention is located at the Modern Agricultural Comprehensive Experimental Demonstration Base of Shandong Academy of Agricultural Sciences in Dongying. The soil physicochemical properties of this area are shown in Table 1.

[0020] Table 1

[0021]

[0022] The plant endophytic lysine spores used in this invention (CGMCC NO.1.15291), budding short-stem mold (CGMCC NO.3.3984), and Frederick rhizobium (CGMCC NO.1.4354) were all purchased from the China General Microbiological Culture Collection Center.

[0023] Example 1

[0024] (1) After sterilizing the mushroom residue at high temperature, adjust the moisture content to 40%, then add 0.12% zinc chloride and 6% propylene glycol by weight of mushroom residue, stir evenly and let stand for 18 hours, then add 15% calcium peroxide by weight of mushroom residue and stir evenly to obtain the auxiliary material.

[0025] (2) Plant endophytic lysine-containing Bacillus, budding short-branched fungus, and Frederick rhizobium were added to Gao's No. 1 medium for activation, amplification, and reproduction. Then, excipients were added (the ratio of each bacterial solution to the excipients was 1:1:1:20) to obtain a compound microbial agent. In this agent, the viable count of each strain was greater than 10. 8 CFU / g;

[0026] (3) Deep plow and rotary till the saline-alkali land, and then spread 80 kg of compound microbial agent on the surface of each mu of saline-alkali land;

[0027] (4) The saline-alkali land is rotary tilled again, irrigated, and then salt-tolerant crops are selected for sowing.

[0028] Comparative Example 1

[0029] (1) After sterilizing the mushroom residue at high temperature, adjust the moisture content to 40%, then add 0.12% zinc chloride and 6% propylene glycol by weight of mushroom residue, stir evenly and let stand for 18 hours, then add 15% calcium peroxide by weight of mushroom residue and stir evenly to obtain the auxiliary material.

[0030] (2) Plant endophytic lysine-containing Bacillus and Frederick Rhizobium were added to Gao's No. 1 medium for activation, amplification and reproduction, and then excipients were added (the ratio of each bacterial solution to the excipients was 1:1:20) to obtain a compound microbial agent;

[0031] (3) Deep plow and rotary till the saline-alkali land, and then spread 80 kg of compound microbial agent on the surface of each mu of saline-alkali land;

[0032] (4) The saline-alkali land is rotary tilled again, irrigated, and then salt-tolerant crops are selected for sowing.

[0033] Comparative Example 2

[0034] (1) After sterilizing the mushroom residue at high temperature, adjust the moisture content to 40%, then add 0.12% zinc chloride and 6% propylene glycol by weight of mushroom residue, stir evenly and let stand for 18 hours, then add 15% calcium peroxide by weight of mushroom residue and stir evenly to obtain the auxiliary material.

[0035] (2) Bud-forming short-branched spores and Rhizobium frutescens were added to Gao's No. 1 medium for activation and amplification, and then excipients were added (the ratio of each bacterial solution to the excipients was 1:1:20) to obtain a compound microbial agent. In this agent, the viable count of each strain was greater than 10. 8 CFU / g;

[0036] (3) Deep plow and rotary till the saline-alkali land, and then spread 80 kg of compound microbial agent on the surface of each mu of saline-alkali land;

[0037] (4) The saline-alkali land is rotary tilled again, irrigated, and then salt-tolerant crops are selected for sowing.

[0038] Comparative Example 3

[0039] (1) After sterilizing the oyster mushroom residue at high temperature, adjust the moisture content to 40%, then add calcium peroxide accounting for 15% of the mushroom weight and stir evenly to obtain the auxiliary material;

[0040] (2) Plant endophytic lysine-containing Bacillus, budding short-branched fungus, and Frederick rhizobium were added to Gao's No. 1 medium for activation, amplification, and reproduction. Then, excipients were added (the ratio of each bacterial solution to the excipients was 1:1:1:20) to obtain a compound microbial agent. In this agent, the viable count of each strain was greater than 10. 8 CFU / g;

[0041] (3) Deep plow and rotary till the saline-alkali land, and then spread 80 kg of compound microbial agent on the surface of each mu of saline-alkali land;

[0042] (4) The saline-alkali land is rotary tilled again, irrigated, and then salt-tolerant crops are selected for sowing.

[0043] Results and Discussion

[0044] (I) The methods provided in Example 1 and Comparative Examples 1-3 were tested in saline-alkali areas. A salt-tolerant peanut variety (Huayu 25) was planted using a ridge-planting and mulching method: before sowing, the land was prepared into ridges, 15 cm high, 50 cm wide, and with furrows 30 cm wide. Two rows of peanuts were sown on the ridges and covered with mulch, with a row spacing of 30 cm. After peanut harvest, soil samples were taken again for relevant physicochemical property testing. The specific results are shown in Table 2.

[0045] Table 2

[0046]

[0047] (ii) The emergence rate and yield of peanuts at harvest were recorded for each experimental field. The specific results are shown in Table 3.

[0048] Table 3

[0049]

Claims

1. A method for improving saline-alkali soil based on microbial inoculants, characterized in that, Includes the following steps: (1) Deep plow and rotary till the saline-alkali land, and then spread compound microbial agents on the surface of the saline-alkali land; (2) The saline-alkali land was rotary tilled again, irrigated, and then salt-tolerant crops were selected for sowing; The compound microbial agent is composed of plant endophytic lysine-containing Bacillus, budding short-stem mold, Rhizobium frutescens, and excipients. The preservation number of the plant endophytic lysine-containing Bacillus is CGMCC NO.1.15291; the preservation number of the budding short-stem fungus is CGMCC NO.3.3984; and the preservation number of the Frederick Rhizobium is CGMCC NO.1.4354. All three strains were purchased from the China General Microbiological Culture Collection Center. The auxiliary material is composed of mushroom residue and calcium peroxide in a mass ratio of 1:0.15; The auxiliary material is prepared by the following method: after sterilizing the mushroom residue at high temperature, the moisture content is adjusted to 35-40%, then zinc chloride and propylene glycol are added, stirred evenly, and left to stand for 15-18 hours. Then calcium peroxide is added and stirred evenly.

2. The method according to claim 1, characterized in that, In the aforementioned compound microbial agent, the viable counts of *Bacillus lysine-containing*, *Bacillus buddingus*, and *Rhizobium frutescens* are all greater than 10. 8 CFU / g.

3. The method according to claim 1, characterized in that, The amount of zinc chloride added accounts for 0.10-0.15% of the mass of the mushroom residue; the amount of propylene glycol added accounts for 5-6% of the mass of the mushroom residue.

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