A method for regulating the flowering period of introducing Ziziphus mauritiana in northern saline-alkali land

Abstract

The present invention belongs to the technical field of jujube tree planting, and particularly relates to a method for regulating the flowering period of big green jujube introduced in northern saline-alkali land. The method comprises the following steps: from March 15th to 20th, place the healthy and well-growing big green jujube trees transplanted into flowerpots in an environment of 4-18 °C for low-temperature treatment for 10-15 days, and keep the temperature difference between day and night at 8-14 °C during this period; spray an aqueous solution of phenylalanine ammonia-lyase on the big green jujube trees during the low-temperature treatment period; on March 30th or 31st, move the treated big green jujube trees in the flowerpots into the greenhouse and bury them in the soil so that the soil in the flowerpots is flush with the ground; in the middle of April, spray an aqueous solution of chalcone synthase on the big green jujube trees in the greenhouse. The method provided by the present invention can systematically advance the flowering period of big green jujube to early June, making it coincide with the flowering period of winter jujube, laying a foundation for the distant hybridization of the two.

Description

Technical Field

[0001] The present invention belongs to the technical field of jujube tree planting, and particularly relates to a method for regulating the flowering period of introducing large green jujubes in northern saline-alkali land. Background Technique

[0002] The large green jujube, also known as the hairy leaf jujube, Indian jujube, Myanmar jujube, etc., is native to tropical regions such as India. The large green jujube has large fruits and a crisp and delicate taste, and its stems, branches, flowers, and leaves all have high utilization values. Since 2021, the Management Service Center of Shandong Binzhou National Agricultural Science and Technology Park and Hebei Agricultural University have cooperated to successfully introduce large green jujube varieties such as Honey Crisp, Milk Jujube, and Taiwan Large Green Jujube from Guangdong. After soil improvement and anti-saline-alkali and cold resistance training, they have all shown good adaptability. The winter jujube is native to Zhanhua District, Binzhou City, Shandong Province. It has a bright ochre-red color, thin skin, crispy flesh, tender and juicy, and is rich in nutrition. It contains 18 kinds of amino acids and mineral elements required by the human body.

[0003] Both the winter jujube and the large green jujube belong to the genus Ziziphus (Rhamnaceae), but at the species level, the winter jujube belongs to the common jujube (Ziziphus jujuba), while the large green jujube is the hairy leaf jujube (Ziziphus mauritiana). Conducting distant hybridization between the two has important practical significance: First, it can combine the excellent genes of the two species to create new genetic variations, thus developing new species. Second, it can cultivate excellent traits such as resistance to pests and diseases, fruit cracking, and saline-alkali, improving the adaptability and stability of jujube trees. In distant hybridization, ensuring that the flowering periods of the male and female parents coincide is the most critical prerequisite. However, the general flowering period of the large green jujube is from July to December, and the full-bloom period of the Zhanhua winter jujube is from April to June. Under natural conditions, the full-bloom periods of the large green jujube and the winter jujube cannot coincide. The biggest obstacle encountered in the cross-breeding work of the large green jujube and the winter jujube by Beijing Forestry University in 2006 was the non-coincidence of the flowering periods. Although the method of collecting winter jujube flower buds in early June, drying and refrigerating them for pollination was adopted, the flowering periods of the winter jujube and the Taiwan green jujube differed too much, and the viability of winter jujube pollen was reduced during storage, ultimately resulting in the failure of hybridization. Therefore, using technical means to make the full-bloom periods of the large green jujube and the winter jujube coincide is the key to determining the success of their distant hybridization. Summary of the Invention

[0004] Aiming at the problems in the prior art that the flowering periods of the winter jujube and the large green jujube do not coincide and fertilization cannot occur after pollination with preserved pollen, the present invention provides a method for regulating the flowering period of introducing large green jujubes in northern saline-alkali land, which can regulate the flowering period of the large green jujube to early June, achieving coincidence with the flowering period of the winter jujube and laying a foundation for their distant hybridization.

[0005] A method for regulating the flowering period of introducing large green jujubes in northern saline-alkali land includes the following steps:

[0006] (1) From March 15th to 20th, place the healthy and well - growing transplanted big green jujube trees in flowerpots in an environment of 4 - 18°C for low - temperature treatment for 10 - 15 days, and keep the day - night temperature difference at 8 - 14°C during this period;

[0007] (2) During the low - temperature treatment, spray an aqueous solution of phenylalanine ammonia - lyase on the big green jujube trees; by mass ratio, the aqueous solution of phenylalanine ammonia - lyase is composed of water and a preparation solution of phenylalanine ammonia - lyase in a ratio of 100:2 - 3;

[0008] (3) On March 30th or 31st, move the big green jujube trees treated in step (2) together with the flowerpots into the greenhouse and bury them in the soil so that the soil in the flowerpots is flush with the ground;

[0009] (4) In mid - April, spray an aqueous solution of chalcone synthase on the big green jujube trees in the greenhouse; by mass ratio, the aqueous solution of chalcone synthase is composed of water and a preparation solution of chalcone synthase in a ratio of 100:1 - 3.

[0010] Preferably, avoid direct sunlight during the low - temperature treatment in step (1).

[0011] Preferably, the water content of the soil in the flowerpots in step (1) is 20 - 30%.

[0012] Preferably, the salt content of the soil in the flowerpots in step (1) is 1.5 - 3.5‰. Moderate drought and increased osmotic pressure and other stress signals in the soil can induce plant flowering. When plants respond to these stress signals, they produce different stress - related plant hormones, such as ethylene, salicylic acid, etc., which ultimately regulate multiple different flowering genes, enabling plants to flower earlier under stress conditions.

[0013] Preferably, the variety of the big green jujube in step (1) is the Taiwan big green jujube. This variety has good quality and broad market prospects.

[0014] Preferably, spray the aqueous solution of phenylalanine ammonia - lyase in step (2) 2 times, with an interval of 5 - 6 days.

[0015] Preferably, from the time when the big green jujube trees are moved into the greenhouse in step (3) until June 1st, control the temperature in the greenhouse at 15 - 30°C, and conduct routine management of the greenhouse after June 1st. Keep sufficient light in the greenhouse. If there are 3 consecutive cloudy days or poor light, supplementary lighting is required. The method of supplementary lighting is to place 1 36w full - spectrum lamp every 5m×5m in the greenhouse.

[0016] Preferably, spray the aqueous solution of chalcone synthase in step (4) 2 times, with an interval of 5 - 6 days.

[0017] The beneficial effects of the present invention are as follows:

[0018] (1) Low-temperature treatment of the large green jujube tree will cause the large green jujube to shed its leaves but not be damaged by freezing, achieving low-temperature induction of the large green jujube to bloom earlier.

[0019] (2) Controlling the soil water content and salt content in the planting flowerpots can regulate plant flowering. When plants respond to salt and drought stress signals, they will produce more plant hormones such as ethylene and salicylic acid. These plant hormones will regulate multiple different flowering genes, thus causing the plants to bloom earlier.

[0020] (3) Phenylalanine ammonia-lyase can promote plants to respond to various biotic and abiotic stresses, and indirectly affect the flowering process of plants; chalcone synthase can improve the stress resistance of plants; the combined use of the two solutions can effectively induce the large green jujube tree to bloom earlier.

[0021] (4) The method provided by the present invention can systematically regulate the flowering period of the large green jujube to be advanced to early June, achieving the encounter with the flowering period of the winter jujube, and laying a foundation for the distant hybridization of the two. Description of the Drawings

[0022] Figure 1 Diagram of the encounter of the flowering periods of the large green jujube and the winter jujube. In the figure, 1 is the flower of the winter jujube, and 2 is the flower of the large green jujube. Detailed Embodiments

[0023] The present invention discloses a method for regulating the flowering period of introducing large green jujubes in northern saline-alkali lands. Those skilled in the art can draw on the content of this article and appropriately improve the process parameters for field operations. It should be particularly noted that all similar substitutions and modifications are obvious to those skilled in the art, and they are all considered to be included in the present invention. The method and application of the present invention have passed relevant tests, and preferred embodiments with better effects have been described. Relevant personnel can obviously make changes or appropriate alterations and combinations to the methods and applications described in this article without departing from the content and scope of the present invention to implement and apply the technology of the present invention.

[0024] Overview of the Test Site

[0025] The test site of this application is located at the Management Service Center of Shandong Binzhou National Agricultural Science and Technology Park, east of Chenjiacun, Botou Town, Zhanhua District, Binzhou City, Shandong Province (37.22°N, 118.02°E). This area belongs to the warm temperate monsoon climate, with obvious continental meteorological characteristics and significant differences in the four seasons. The annual average sunshine hours are 2,690.3 hours. Among the sunshine hours within a year, May is the largest and December is the smallest.

[0026] Varieties of Large Green Jujube Trees

[0027] This application selects the Taiwan large green jujube tree, which is from Meitian Village, Zhongluotan Town, Baiyun District, Guangzhou City, Guangdong Province. It is a 4-year-old tree with a trunk height of 1.7 m and a diameter at breast height of 3 cm.

[0028] Preparation of Phenylalanine Ammonia-Lyase and Chalcone Synthase

[0029] (I) Preparation of Phenylalanine ammonia-lyase (PAL) includes the following steps:

[0030] (1) Select and prepare materials: Wash and chop wheat grains;

[0031] (2) Lyse plant tissues: Mix wheat grain fragments with a phenylalanine ammonia-lyase extraction reagent (brand: Regen Biotech) in a ratio of 10 kg of tissue: 10 - 15 L of extraction reagent, and grind thoroughly under ice bath conditions;

[0032] (3) Separate and purify: Centrifuge the mixture at 4°C for 15 - 20 minutes to separate the supernatant, which is the phenylalanine ammonia-lyase preparation solution;

[0033] (4) Preservation: The extracted and purified PAL enzyme should be stored under appropriate conditions, usually frozen at -20°C or -80°C to maintain its activity.

[0034] (II) Preparation of chalcone synthase (CHS) includes the following steps:

[0035] (1) RNA extraction: Use the ESPY spin plant RNA rapid extraction kit to extract total RNA from Glycyrrhiza uralensis Fisch;

[0036] (2) Reverse transcription: Using the extracted RNA as a template, use reverse transcriptase (M-MLV(Rnase H-)) and Oligo dT as primers to synthesize cDNA;

[0037] (3) Gene cloning: Design specific primers according to the known CHS gene sequence, amplify the target gene by PCR, and purify it using an agarose gel purification and recovery kit;

[0038] (4) Vector construction: ligate the purified CHS gene fragment into a suitable expression vector, such as the pMD-19T vector;

[0039] (5) Transformation: Transform the ligation product into Escherichia coli and screen for clones containing the recombinant plasmid;

[0040] (6) Sequence verification: Sequence the positive clones to verify the correctness of the CHS gene sequence;

[0041] (7) Protein expression: Transform the verified plasmid into an expression host cell to induce protein expression. Cell lysis: Use lysis buffer to lyse the cells and release the CHS protein;

[0042] (8) Protein purification: The preparation solution of chalcone synthase can be obtained by purification through ion exchange chromatography.

[0043] Example 1

[0044] A method for regulating the flowering period of introduced Taiwan big jujube trees in the saline-alkali land in the north, comprising the following steps:

[0045] (1) On March 20th, place the healthy and well-growing transplanted Taiwan big jujube trees in flowerpots in an environment of 4 - 12 °C for low-temperature treatment for 10 days. During this period, keep the temperature difference between day and night at 8 °C and avoid direct sunlight; the water content of the soil in the flowerpots is 20 - 24%, and the salt content is 1.5‰;

[0046] (2) During the low-temperature treatment, spray the aqueous solution of phenylalanine ammonia-lyase on the big jujube trees 2 times at an interval of 5 days. By mass ratio, the aqueous solution of phenylalanine ammonia-lyase is composed of water and the preparation solution of phenylalanine ammonia-lyase according to 100:2;

[0047] (3) On March 30th, move the big jujube trees treated in step (2) together with the flowerpots into the greenhouse and bury them in the soil so that the soil in the flowerpots is flush with the ground. Control the temperature in the greenhouse at 15 - 30 °C from March 30th to June 1st, and conduct routine management of the greenhouse after June 1st; keep sufficient light in the greenhouse. If there are 3 consecutive cloudy days or poor light, supplementary lighting is required. Place 1 36w full-spectrum lamp every 5m × 5m in the greenhouse;

[0048] (4) Starting from April 10th, start spraying the aqueous solution of chalcone synthase on the big jujube trees in the greenhouse 2 times at an interval of 5 days; by mass ratio, the aqueous solution of chalcone synthase is composed of water and the preparation solution of chalcone synthase according to 100:2.

[0049] Example 2

[0050] A method for regulating the flowering period of introduced Taiwan big jujube trees in the saline-alkali land in the north, comprising the following steps:

[0051] (1) On March 16th, place the healthy and well-growing transplanted Taiwan big jujube trees in flowerpots in an environment of 6 - 18 °C for low-temperature treatment for 15 days. During this period, keep the temperature difference between day and night at 8 - 12 °C and avoid direct sunlight; the water content of the soil in the flowerpots is 23 - 28%, and the salt content is 1.7‰;

[0052] (2) During the low-temperature treatment, spray the aqueous solution of phenylalanine ammonia-lyase on the big jujube trees 2 times at an interval of 5 days. By mass ratio, the aqueous solution of phenylalanine ammonia-lyase is composed of water and the preparation solution of phenylalanine ammonia-lyase according to 100:2;

[0053] (3) On March 31, move the big green jujube tree after being processed in step (2) into the greenhouse with the pot and bury it in the soil so that the soil in the flower pot is flush with the ground. Control the temperature in the greenhouse at 15 - 30 °C from March 31 to June 1, and conduct routine management of the greenhouse after June 1; keep sufficient light in the greenhouse. If there are three consecutive cloudy days or poor light, supplementary lighting is required. Place 1 36-watt full-spectrum lamp every 5m × 5m in the greenhouse;

[0054] (4) Starting from April 15, spray the chalcone synthase aqueous solution on the big green jujube trees in the greenhouse twice with a 5-day interval; by mass ratio, the chalcone synthase aqueous solution is prepared from water and a chalcone synthase preparation solution in a ratio of 100:2.

[0055] Example 3

[0056] A method for regulating the flowering period of big green jujube introduced in northern saline-alkali land includes the following steps:

[0057] (1) On March 20, place the healthy and well-growing Taiwan big green jujube tree transplanted into a flower pot in an environment of 8 - 18 °C for low-temperature treatment for 11 days, keeping the temperature difference between day and night at 8 - 10 °C during this period and avoiding direct sunlight; the soil moisture content in the flower pot is 28 - 30%, and the salt content is 2‰;

[0058] (2) During the low-temperature treatment, spray the phenylalanine ammonia-lyase aqueous solution on the big green jujube tree twice with a 5-day interval. By mass ratio, the phenylalanine ammonia-lyase aqueous solution is prepared from water and a phenylalanine ammonia-lyase preparation solution in a ratio of 100:3;

[0059] (3) On March 31, move the big green jujube tree after being processed in step (2) into the greenhouse with the pot and bury it in the soil so that the soil in the flower pot is flush with the ground. Control the temperature in the greenhouse at 15 - 30 °C from March 31 to June 1, and conduct routine management of the greenhouse after June 1; keep sufficient light in the greenhouse. If there are three consecutive cloudy days or poor light, supplementary lighting is required. Place 1 36-watt full-spectrum lamp every 5m × 5m in the greenhouse;

[0060] (4) Starting from April 20, spray the chalcone synthase aqueous solution on the big green jujube trees in the greenhouse twice with a 5-day interval; by mass ratio, the chalcone synthase aqueous solution is prepared from water and a chalcone synthase preparation solution in a ratio of 100:3.

[0061] Comparative Example 1

[0062] A method for regulating the flowering period of big green jujube introduced in northern saline-alkali land includes the following steps:

[0063] (1) On March 16th, transplant healthy and well-growing Taiwan big green jujube trees that have been transplanted into flower pots into an environment of 6°C - 18°C for low-temperature treatment for 15 days. During this period, maintain a temperature difference of 8 - 12°C between day and night, and avoid direct sunlight; the water content of the soil in the flower pot is 40 - 50%, and the salt content is 1.0‰;

[0064] (2) During the low-temperature treatment period, spray the big green jujube trees with phenylalanine ammonia-lyase aqueous solution twice, with an interval of 5 days. By mass ratio, the phenylalanine ammonia-lyase aqueous solution is composed of water and phenylalanine ammonia-lyase preparation solution in a ratio of 100:2;

[0065] (3) On March 31st, move the big green jujube trees treated in step (2) together with the flower pots into the greenhouse and bury them in the soil so that the soil in the flower pot is level with the ground. From March 31st to June 1st, control the temperature in the greenhouse at 15 - 30°C, and conduct regular management of the greenhouse after June 1st; keep sufficient light in the greenhouse. If there are three consecutive cloudy days or poor light, supplementary lighting is required. Place one 36w full-spectrum lamp every 5m×5m in the greenhouse;

[0066] (4) Starting from April 15th, spray the big green jujube trees in the greenhouse with chalcone synthase aqueous solution twice, with an interval of 5 days; by mass ratio, the chalcone synthase aqueous solution is composed of water and chalcone synthase preparation solution in a ratio of 100:2.

[0067] Comparative Example 2

[0068] A method for regulating the flowering period of introducing big green jujube in northern saline-alkali land, comprising the following steps:

[0069] (1) On March 16th, transplant healthy and well-growing Taiwan big green jujube trees that have been transplanted into flower pots into an environment of 6 - 18°C for low-temperature treatment for 15 days. During this period, maintain a temperature difference of 8 - 12°C between day and night, and avoid direct sunlight; the water content of the soil in the flower pot is 23 - 28%, and the salt content is 1.7‰;

[0070] (2) During the low-temperature treatment period, spray the big green jujube trees with phenylalanine ammonia-lyase aqueous solution twice, with an interval of 5 days. By mass ratio, the phenylalanine ammonia-lyase aqueous solution is composed of water and phenylalanine ammonia-lyase preparation solution in a ratio of 100:2.5;

[0071] (3) On March 31st, move the big green jujube trees treated in step (2) together with the flower pots into the greenhouse and bury them in the soil so that the soil in the flower pot is level with the ground. From March 31st to June 1st, control the temperature in the greenhouse at 15 - 30°C, and conduct regular management of the greenhouse after June 1st. Keep sufficient light in the greenhouse. If there are three consecutive cloudy days or poor light, supplementary lighting is required. Place one 36w full-spectrum lamp every 5m×5m in the greenhouse.

[0072] Comparative Example 3

[0073] A method for regulating the flowering period of introducing big green jujube in northern saline-alkali land, comprising the following steps:

[0074] (1) On March 16th, place the healthy and well-growing Taiwan big green jujube trees transplanted into flowerpots in an environment of 6-18°C for 15 days of low-temperature treatment. During this period, maintain a temperature difference of 8-12°C between day and night and avoid direct sunlight; the water content of the soil in the flowerpots is 23-28%, and the salt content is 1.7‰;

[0075] (2) On March 31st, move the big green jujube trees treated in step (2) together with the flowerpots into the greenhouse and bury them in the soil. The soil in the flowerpots is flush with the ground. Control the temperature in the greenhouse at 15-30°C from March 31st to June 1st. After June 1st, conduct regular management of the greenhouse. Keep sufficient light in the greenhouse. If there are 3 consecutive cloudy days or poor light, supplementary lighting is required. The supplementary lighting method is to place 1 36w full-spectrum lamp every 5m×5m in the greenhouse.

[0076] Comparative Example 4

[0077] A method for regulating the flowering period of introducing big green jujube in northern saline-alkali land, comprising the following steps:

[0078] (1) On February 1st, place the healthy and well-growing Taiwan big green jujube trees transplanted into flowerpots in an environment of -4-4°C for 15 days of low-temperature treatment. During this period, maintain a temperature difference of 8°C between day and night and avoid direct sunlight; the water content of the soil in the flowerpots is 23-28%, and the salt content is 1.7‰;

[0079] (2) During the low-temperature treatment period, spray the big green jujube trees with phenylalanine ammonia-lyase aqueous solution 2 times at an interval of 5 days. By mass ratio, the phenylalanine ammonia-lyase aqueous solution is prepared from water and phenylalanine ammonia-lyase preparation solution in a ratio of 100:2;

[0080] (3) On February 16th, move the big green jujube trees treated in step (2) together with the flowerpots into the greenhouse and bury them in the soil. The soil in the flowerpots is flush with the ground. Control the greenhouse temperature at 15-28°C. Control the temperature in the greenhouse at 15-30°C from February 16th to June 1st. After June 1st, conduct regular management of the greenhouse; keep sufficient light in the greenhouse. If there are 3 consecutive cloudy days or poor light, supplementary lighting is required; place 1 36w full-spectrum lamp every 5m×5m in the greenhouse;

[0081] (4) Starting from April 15th, spray the big green jujube trees in the greenhouse with chalcone synthase aqueous solution 2 times at an interval of 5 days; by mass ratio, the chalcone synthase aqueous solution is prepared from water and chalcone synthase preparation solution in a ratio of 100:1-3.

[0082] Comparative Example 5

[0083] A method for regulating the flowering period of introducing big green jujube in northern saline-alkali land, comprising the following steps:

[0084] (1) On March 16th, transplant healthy and well - growing Taiwan big green jujube trees that have been transplanted into flowerpots to a low - temperature environment of 15 - 25°C for 15 days. During this period, maintain a temperature difference between day and night of 8 - 10°C and avoid direct sunlight. The water content of the soil in the flowerpot is 23 - 28%, and the salt content is 1.7‰.

[0085] (2) During the low - temperature treatment, spray the phenylalanine ammonia - lyase aqueous solution on the big green jujube trees 2 times at an interval of 5 days. By mass ratio, the phenylalanine ammonia - lyase aqueous solution is composed of water and phenylalanine ammonia - lyase preparation solution in a ratio of 100:2.

[0086] (3) On March 31st, move the big green jujube trees treated in step (2) together with the flowerpots into the greenhouse and bury them in the soil so that the soil in the flowerpot is level with the ground. Control the temperature in the greenhouse at 15 - 30°C from March 31st to June 1st, and conduct routine management of the greenhouse after June 1st.

[0087] (4) Starting from April 15th, spray the chalcone synthase aqueous solution on the big green jujube trees in the greenhouse 2 times at an interval of 5 days. By mass ratio, the chalcone synthase aqueous solution is composed of water and chalcone synthase preparation solution in a ratio of 100:2.

[0088] Comparative Example 6

[0089] A method for regulating the flowering period of introducing big green jujubes in northern saline - alkali land, comprising the following steps:

[0090] (1) On March 16th, transplant healthy and well - growing Taiwan big green jujube trees that have been transplanted into flowerpots to a low - temperature environment of 6 - 18°C for 15 days. During this period, maintain a temperature difference between day and night of 8 - 12°C and avoid direct sunlight. The water content of the soil in the flowerpot is 23 - 28%, and the salt content is 0.8‰.

[0091] (2) During the low - temperature treatment, spray the phenylalanine ammonia - lyase aqueous solution on the big green jujube trees 2 times at an interval of 5 days. By mass ratio, the phenylalanine ammonia - lyase aqueous solution is composed of water and phenylalanine ammonia - lyase preparation solution in a ratio of 100:2.

[0092] (3) On March 31st, move the big green jujube trees treated in step (2) together with the flowerpots into the greenhouse and bury them in the soil so that the soil in the flowerpot is level with the ground. Control the temperature in the greenhouse at 15 - 30°C from March 31st to June 1st, and conduct routine management of the greenhouse after June 1st. Keep sufficient light in the greenhouse. If there are 3 consecutive cloudy days or poor light, supplementary lighting is required. Place 1 36 - w full - spectrum lamp every 5m×5m in the greenhouse.

[0093] (4) Starting from April 15th, spray the chalcone synthase aqueous solution on the big green jujube trees in the greenhouse 2 times at an interval of 5 days. By mass ratio, the chalcone synthase aqueous solution is composed of water and chalcone synthase preparation solution in a ratio of 100:2.

[0094] Comparative Example 7

[0095] A method for regulating the flowering period of introducing Ziziphus mauritiana in northern saline-alkali land, comprising the following steps:

[0096] (1) On March 16th, place the healthy and well-growing Taiwan Ziziphus mauritiana transplanted into flowerpots in an environment of 6 - 18°C for low-temperature treatment for 6 days. During this period, keep the temperature difference between day and night at 8 - 12°C and avoid direct sunlight; the water content of the soil in the flowerpots is 23 - 28%, and the salt content is 1.7‰;

[0097] (2) During the low-temperature treatment, spray the phenylalanine ammonia-lyase aqueous solution on the Ziziphus mauritiana twice at an interval of 5 days. By mass ratio, the phenylalanine ammonia-lyase aqueous solution is composed of water and phenylalanine ammonia-lyase preparation solution in a ratio of 100:2;

[0098] (3) On March 22nd, move the Ziziphus mauritiana treated in step (2) together with the flowerpot into the greenhouse and bury it in the soil so that the soil in the flowerpot is flush with the ground. Control the temperature in the greenhouse at 15 - 30°C from March 22nd to June 1st, and conduct regular management of the greenhouse after June 1st;

[0099] (4) Starting from April 15th, spray the chalcone synthase aqueous solution on the Ziziphus mauritiana in the greenhouse twice at an interval of 5 days; by mass ratio, the chalcone synthase aqueous solution is composed of water and chalcone synthase preparation solution in a ratio of 100:2.

[0100] Observe the flowering time of Ziziphus mauritiana in Examples 1 - 3 and Comparative Examples 1 - 7 to determine its flowering period.

[0101] Flowering period investigation of Ziziphus mauritiana in this application

[0102]

[0103] Note: The Ziziphus mauritiana in Comparative Example 4 did not survive.

[0104] Figure 1 For the picture of the flowering period encounter between the Ziziphus mauritiana in Example 2 and winter jujube, it was taken on June 8, 2024. From Figure 1 It can be seen from the flowering period investigation form of Ziziphus mauritiana that after regulating the flowering period of Ziziphus mauritiana by the method provided in the present invention, the flowering period of Ziziphus mauritiana was successfully advanced to early June, achieving the encounter with the flowering period of winter jujube, thus laying a foundation for the distant hybridization of the two.

Claims

1. A method for regulating the flowering period of jujube introduced into northern saline-alkali land, characterized in that: The steps include: (1) From March 15 to 20, healthy and well-growing jujube trees that have been transplanted into flower pots are placed in a low temperature treatment environment of 4-18°C for 10-15 days, during which the temperature difference between day and night is maintained at 8-14°C; the soil moisture content in the flower pots is 20-30%, and the soil salt content in the flower pots is 1.5-3.5‰; (2) spraying the jujube trees with an aqueous solution of phenylalanine ammonia lyase during the low temperature treatment; the aqueous solution of phenylalanine ammonia lyase is composed of water and phenylalanine ammonia lyase preparation solution in a ratio of 100:2-3 by mass; (3) On March 30 or 31, move the jujube trees treated in step (2) with their pots into the greenhouse and bury them in the soil so that the soil in the pots is flush with the ground; (4) In mid-April, the jujube trees in the greenhouse were sprayed with a chalcone synthase aqueous solution; the chalcone synthase aqueous solution was composed of water and chalcone synthase preparation solution in a ratio of 100:1-3 by mass.

2. The method for regulating flowering period according to claim 1, characterized in that: During step (1) the low temperature treatment, avoid direct sunlight.

3. The method for regulating flowering period according to claim 1, characterized in that: The variety of the jujube tree in step (1) is Taiwan jujube tree.

4. The method for regulating flowering period according to claim 1, characterized in that: Step (2) spraying the phenylalanine ammonia lyase aqueous solution twice, with an interval of 5-6 days.

5. The method for regulating flowering period according to claim 1, characterized in that: In step (3), the jujube trees are moved into the greenhouse and the temperature in the greenhouse is controlled at 15-30°C until June 1, and routine management of the greenhouse is performed after June 1.

6. The method for regulating flowering period according to claim 5, characterized in that: Maintain sufficient light in the greenhouse. If it is cloudy for three consecutive days or the light is poor, supplementary light is needed.

7. The method for regulating flowering period according to claim 6, characterized in that: The supplementary lighting method is to place a 36w full-spectrum lamp every 5m×5m in the greenhouse.

8. The method for regulating flowering period according to claim 1, characterized in that: Step (4) spraying the chalcone synthase aqueous solution twice, with an interval of 5-6 days.

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