Fig meal replacement powder as well as preparation method and application thereof
A fig-based meal replacement powder with specific ingredients addresses oxidative stress and obesity by providing strong antioxidant capacity and restoring blood lipid levels, effectively reducing obesity-related oxidative stress and improving liver health.
Patent Information
- Application Number
- CN202411299195.4
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-09-18
- Publication Date
- 2024-12-06
AI Technical Summary
Current meal replacement powders primarily focus on weight loss through satiety and reduced carbohydrate and fat intake, lacking those that alleviate oxidative stress damage caused by obesity, a common metabolic disorder leading to chronic diseases.
A fig meal replacement powder composed of figs, mulberries, yam, black sesame, red beans, and brown rice, prepared by crushing and sieving, with a specific weight ratio, offering high antioxidant capacity and oxidative stress relief.
The fig meal replacement powder effectively scavenges free radicals, reduces oxidative stress, and improves obesity by restoring blood lipid levels, increasing HDL-C, and enhancing antioxidant enzyme activities in the liver.
Smart Images
Figure CN119073543A_ABST
Abstract
Description
Technical Field
[0001] The invention relates to the field of functional foods, in particular to a fig meal replacement powder and a preparation method and application thereof. Background Art
[0002] Figs are rich in nutrients, containing a variety of nutrients and a large number of functional ingredients. They have multiple functions such as antioxidant, lowering blood sugar, lowering blood lipids and lowering blood pressure. They have high edible and medicinal value. Therefore, developing products using figs as raw materials has great economic value.
[0003] Obesity, as one of the most common metabolic diseases, can lead to a variety of chronic diseases such as type 2 diabetes, non-alcoholic fatty liver disease, cardiovascular disease, etc. The fatty acid metabolism of the liver of obese people will be disordered, and a large number of free radicals will be produced in the liver, which will induce liver oxidative stress and damage the liver. Supplementing with foods with high antioxidant capacity can help the body to scavenge free radicals, relieve oxidative stress, and relieve metabolic diseases such as obesity mediated by oxidative stress. Therefore, anti-oxidative stress is an important way to prevent and relieve obesity and its complications. The development of a functional food that can relieve the oxidative stress damage to the body caused by obesity has certain practical significance for preventing and relieving obesity and its complications.
[0004] Meal replacement powder is one of the most common meal replacement products, with the characteristics of low calories and comprehensive nutrition. Currently, the meal replacement powders on the market mainly achieve weight loss effects by enhancing satiety and reducing carbohydrate and fat intake, while meal replacement powders that can alleviate the oxidative stress damage caused by obesity are relatively rare. Therefore, the development of a meal replacement powder that can improve obesity and alleviate the oxidative stress damage caused by obesity has a good market prospect. Summary of the invention
[0005] Technical problem to be solved: The present invention utilizes fruit and vegetable raw materials such as figs to develop a functional meal replacement powder with strong antioxidant capacity, which can improve obesity and alleviate the body's oxidative stress caused by obesity, as well as its preparation method and application.
[0006] Technical solution: A fig meal replacement powder, the raw materials of which include figs, mulberries, yam, black sesame, red beans and brown rice, the weight proportion of each raw material being 5 to 10 parts of figs, 3 to 6 parts of mulberries, 3 to 6 parts of yam, 1 to 3 parts of black sesame, 2 to 5 parts of red beans and 1 to 3 parts of brown rice.
[0007] Preferably, the weight fractions of the raw materials are 7 parts of figs, 4 parts of mulberries, 3 parts of yam, 1 part of black sesame, 4 parts of red beans, and 1 part of brown rice.
[0008] The preparation method of the fig meal replacement powder comprises the following steps: accurately weighing the raw materials according to the weight ratio, crushing the raw materials by breaking the wall, taking out the coarse powder and passing it through an 80-mesh sieve to obtain the meal replacement powder.
[0009] Application of the fig meal replacement powder in the preparation of food for improving obesity.
[0010] Application of the fig meal replacement powder in preparing foods for lowering blood lipids.
[0011] Application of the fig meal replacement powder in preparing cholesterol-lowering food.
[0012] Application of the fig meal replacement powder in the preparation of antioxidant foods.
[0013] The fig meal replacement powder can be consumed by mixing it with 90°C hot water in a material-to-water ratio of 1:10.
[0014] Beneficial effects: The scheme of the present invention is scientific and reasonable. Through the present invention, a functional meal replacement powder with the function of improving obesity and relieving the oxidative stress damage caused by obesity is disclosed. The present invention has high antioxidant quality, that is, it has strong DPPH free radical scavenging ability, ABTS + Free radical scavenging ability, Fe 2+ Reducing ability. The present invention can also improve obesity and alleviate oxidative stress damage in the body, and can restore blood lipid levels, including reducing the total cholesterol (TC) content, triglyceride (TG) content and low-density lipoprotein cholesterol (LDL-C) in the blood, and increasing high-density lipoprotein cholesterol (HDL-C), thereby improving obesity. In addition, it increases the glutathione (GSH) content, superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px) activity in the liver, reduces the malondialdehyde (MDA) content in the liver, and alleviates liver oxidative stress damage in obese mice. BRIEF DESCRIPTION OF THE DRAWINGS
[0015] Figure 1 Figure 3. Body weight changes of mice.
[0016] Figure 2 Graph showing changes in food intake in mice.
[0017] Figure 3 Changes in blood lipid levels in mice, including (a) serum total cholesterol (TC) level; (b) serum triglyceride (TG) level; (c) serum low-density lipoprotein cholesterol (LDL-C) level; (d) serum high-density lipoprotein cholesterol (HDL-C) level.
[0018] Figure 4 Changes in oxidative stress levels in mouse liver, including (a) superoxide dismutase (SOD) level; (b) malondialdehyde (MDA) level; (c) catalase (CAT) level; (d) glutathione (GSH) level; (e) glutathione peroxidase (GSH-Px) level. DETAILED DESCRIPTION
[0019] Example 1
[0020] Figs, mulberries, yam, brown rice, red beans, wolfberries, lilies, and quinoa were crushed and packed for later use. Black sesame and chia seeds were crushed until the powder particles were uniform and there were no obvious coarse particles. Different meal replacement powder combinations were prepared according to Table 1. 90°C hot water was taken and mixed at a material-water mass ratio of 1:10. After stirring evenly, they were diluted to 2.5 mg / mL and 5 mg / mL respectively. 2.5 mg / mL solutions of different fig meal replacement powder combinations were taken for DPPH free radical scavenging ability determination, and 5 mg / mL solutions of different fig meal replacement powder combinations were taken for ABTS + Free radical scavenging ability and Fe 2+ The results of the reduction capacity determination are shown in Table 1. As shown in Table 1, in the formula of fig meal replacement powder with different ingredients in the same combination ratio and the same ingredients in different combination ratios, when the mass ratio of fig, mulberry, red bean, yam, black sesame and brown rice is 7:4:4:3:1:1, the antioxidant capacity of fig meal replacement powder is the strongest, and the DPPH free radical scavenging capacity reaches 702.61±47.16μg Trolox / mL, and the ABTS + The free radical scavenging capacity reached 9.89±0.26mM TEAC, Fe 2+ The reducing capacity reached 12.54±0.18mM FeSO4 / mL.
[0021] Table 1 Determination results of antioxidant quality of different meal replacement powder combinations
[0022]
[0023] Example 2
[0024] 1. Preparation of Fig Meal Replacement Powder
[0025] Weigh figs, mulberries, adzuki beans, yam, black sesame, and brown rice and mix them in a weight ratio of 7:4:4:3:1:1. After breaking the wall and crushing, pass through an 80-mesh sieve to obtain a uniform meal replacement powder, which is then bagged for later use.
[0026] 2. Preparation of Fig Meal Replacement Powder Solution
[0027] Take an appropriate amount of meal replacement powder, mix it with 90℃ hot water at a material-water mass ratio of 1:10, stir evenly to obtain a fig meal replacement powder solution, and use it immediately after preparation.
[0028] 3. Preparation of commercially available weight loss meal replacement powder solution
[0029] Take commercially available meal replacement powder, add 90°C hot water, and mix it in a material-to-water ratio of 1:10. Stir evenly to obtain a commercially available meal replacement powder solution, which can be prepared and used immediately.
[0030] 4. Determination of antioxidant quality of meal replacement powder
[0031] Take an appropriate amount of meal replacement powder solution and dilute it to 2.5 mg / mL and 5 mg / mL respectively. Take 2.5 mg / mL of fig meal replacement powder solution to test the DPPH free radical scavenging ability. Take 5 mg / mL of fig meal replacement powder solution to test the ABTS + Free radical scavenging ability and Fe 2+ The reducing ability was determined according to the kit instructions. The results are shown in Table 2.
[0032] Table 2 Results of antioxidant quality test of meal replacement powder
[0033]
[0034] 5. Animal Grouping and Obese Mouse Modeling
[0035] Twenty-eight healthy C57BL / 6 female mice aged 5 weeks were selected. The laboratory adopted a 12-hour light-dark rotation lighting, with good ventilation and sanitary conditions, room temperature of 22±2℃, and humidity of 60±5%. First, the experimental mice were fed with ordinary feed for 1 week of environmental adaptation, during which the mice were free to eat ordinary feed and drink water.
[0036] Then, 28 mice were randomly divided into 4 groups. There was no significant difference in the average body weight among the groups (P>0.05). The blank group (NC) continued to be fed with ordinary feed (20% flour, 10% rice flour, 20% corn, 26% drum skin, 20% soybean feed, 2% fish meal, and 2% bone meal). The model group (HFD), positive control group (PC) and experimental group (WHG) were fed with high-fat feed (78.80% high-fat feed, 10% lard, 10% egg yolk powder, 1% cholesterol, and 0.2% bile salt). Four weeks after modeling, the PC group and the WHG group were gavaged with commercial meal replacement powder solution and fig meal replacement powder solution, respectively. The NC group and the HFD group were gavaged with normal saline at a dose of 0.25 mL / 10 g·d -1 The intervention time was 4 weeks. The groups and treatments are shown in Table 2.
[0037] Table 2 Animal experiment groups
[0038]
[0039] 6. Detection of obesity and oxidative stress damage in mice
[0040] (1) Changes in mouse body weight and food intake
[0041] The mice were weighed weekly, and their food intake was recorded daily to draw a trend graph of their weight and food intake.
[0042] (2) Blood lipid levels
[0043] After the intervention, all mice were fasted for 12 h. After ether anesthesia, the eyeballs were immediately removed to collect blood. The collected blood was incubated in a 37°C incubator for 30 min and centrifuged at 3500 rpm and 4°C for 10 min to separate serum. The levels of total cholesterol (TC), triglycerides (TG), low-density lipoprotein cholesterol (LDL-C), and high-density lipoprotein cholesterol (HDL-C) were analyzed using a Hitachi 7020 fully automatic biochemical analyzer.
[0044] (3) Liver oxidative stress level
[0045] After killing the mice, 1.0 g of liver was taken and placed in a homogenizer in an ice bath, 10 mL of PBS solution was added, and the liver tissue was fully pulverized and transferred to a 15 mL centrifuge tube, centrifuged at 3000 r / min for 15 min, and the supernatant of the homogenate after centrifugation was transferred to a 15 mL centrifuge tube and stored at 4 ° C for later use. The supernatant of the liver homogenate was taken, and the activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT) in the liver homogenate, as well as the contents of glutathione (GSH) and malondialdehyde (MDA) were determined by the kit.
[0046] 7. Experimental Results
[0047] (1) Overview of mouse body weight and food intake
[0048] The weight changes of mice Figure 1 As shown in Figure 2, the changes in food intake after oral gavage intervention were as follows: Figure 2 As shown. Figure 1 It can be seen that after 8 weeks of high-fat diet feeding, the weight gain of mice in the HFD group reached 7.01±0.25g, which was significantly higher than that in the NC group (P<0.05). After intervention, the weight gain of the WHG group decreased to 1.58±0.61g, which was significantly lower than that in the HFD group (P<0.05). Figure 2 It can be seen that after 4 weeks of gavage intervention, the daily food intake of mice in the WHG group decreased to 1.31±0.23g / d, which was significantly lower than that in the NC group, HFD group and PC group (P<0.05). In summary, this functional meal replacement powder has a certain weight loss effect and a certain satiety.
[0049] (2) Overview of blood lipid levels in mice
[0050] The lipid levels in mice Figure 3As shown in the results, after being fed with a high-fat diet, the serum TC, TG, and LDL-C levels of mice in the HFD group reached 5.46±0.29mmol / L, 1.06±0.11mmol / L, and 1.01±0.06mmol / L, respectively, which were significantly higher than those in the NC group (P<0.05), and the HDL-C reached 1.58±0.09mmol / L, which was significantly lower than that in the NC group (P<0.05). Compared with the HFD group, the serum TC, TG, and LDL-C levels of the WHG group, which had been gavaged with meal replacement powder, decreased to 4.44±0.24mmol / L, 0.60±0.07mmol / L, and 0.75±0.09mmol / L, respectively, which were significantly lower than those in the HFD group (P<0.05). The HDL-C level increased to 2.14±0.12mmol / L, which was significantly higher than that in the HFD group (P<0.05). The results show that the product has the effect of regulating blood lipid levels.
[0051] (3) Overview of oxidative stress levels in mouse liver
[0052] Antioxidant enzyme activities and malondialdehyde and glutathione contents in mouse liver Figure 4 As shown in the data, the antioxidant activity of the liver of mice in the HFD group was significantly decreased (P<0.05), with the activities of SOD, CAT, and GSH-Px decreased to 150.85±19.13U / mgprot, 0.94±0.72U / mgprot, and 110.95±15.46U / mgprot, respectively, the GSH content decreased to 12.08±1.2μg / mg, and the MDA content increased to 9.73±1.35nmol / mgprot. After intragastric administration of the meal replacement powder solution, the activities of SOD, CAT, GSH-Px and GSH content recovered to 231.99±22.24U / mg prot, 3.43±0.52U / mg prot, 214.67±12.3U / mgprot and 22.88±4.47nmol / mg prot, respectively, which were significantly higher than those in the HFD group (P<0.05), and the MDA content recovered to 1.15±0.22μg / mg, which was significantly lower than that in the HFD group (P<0.05). Among them, the activities of SOD and CAT and the contents of MDA and GSH in the WHG group were not significantly different from those in the NC group (P>0.05), indicating that the functional meal replacement powder has the effect of alleviating oxidative stress damage in the body.
[0053] The above examples are only for illustrating the technical concept and features of the present invention, and their purpose is to enable people familiar with the technology to understand the content of the present invention and implement it accordingly, and they cannot be used to limit the protection scope of the present invention. Any equivalent transformation or modification made according to the spirit of the present invention should be included in the protection scope of the present invention.
Claims
1. A fig meal replacement powder, characterized in that: The raw materials include figs, mulberries, yam, black sesame, red beans and brown rice, and the weight proportion of each raw material is 5-10 parts of figs, 3-6 parts of mulberries, 3-6 parts of yam, 1-3 parts of black sesame, 2-5 parts of red beans and 1-3 parts of brown rice.
2. The fig meal replacement powder according to claim 1, characterized in that: The weight fractions of the raw materials are 7 parts of figs, 4 parts of mulberries, 3 parts of yam, 1 part of black sesame, 4 parts of red beans, and 1 part of brown rice.
3. The method for preparing the fig meal replacement powder according to claim 1 or 2, characterized in that: Accurately weigh each raw material according to the weight ratio, break the wall and grind it, take out the coarse powder and pass it through an 80-mesh sieve to obtain the meal replacement powder.
4. Use of the fig meal replacement powder according to claim 1 or 2 in preparing food for improving obesity.
5. Use of the fig meal replacement powder according to claim 1 or 2 in preparing foods for lowering blood lipids.
6. Use of the fig meal replacement powder according to claim 1 or 2 in preparing cholesterol-lowering food.
7. Use of the fig meal replacement powder according to claim 1 or 2 in the preparation of antioxidant foods.
8. The method for consuming the fig meal replacement powder according to claim 1 or 2, characterized in that: Use 90℃ hot water in a material-to-water ratio of 1:10 for consumption.